Clc10G05890 (gene) Watermelon (cordophanus) v2

BLAST of Clc10G05890 vs. NCBI nr
Match: XP_038905752.1 (probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida])

HSP 1 Score: 995.3 bits (2572), Expect = 2.0e-286
Identity = 491/534 (91.95%), Postives = 514/534 (96.25%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGNNTINKWCKTTPHPKVCISFMSPIKPPPLNRPEFRTM 60
           MASH KILFFSITLFSILSN+S +N+INKWCKTTPHPK+CI FMSPIKPPP NRPEFR M
Sbjct: 1   MASHNKILFFSITLFSILSNVS-SNSINKWCKTTPHPKICIYFMSPIKPPPQNRPEFRKM 60

Query: 61  AIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQ 120
           AIRTA +KA EAQ YATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCV+SDGTI+
Sbjct: 61  AIRTALQKATEAQTYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVISDGTIR 120

Query: 121 RRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAINGVFL- 180
            RRCTDFDAQTWLSSALTDIDLC+SGAADLNVTDFIAPIKCQNVSKMISNCLA+NG FL 
Sbjct: 121 HRRCTDFDAQTWLSSALTDIDLCVSGAADLNVTDFIAPIKCQNVSKMISNCLAVNGRFLE 180

Query: 181 KEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTVQAAID 240
           +E  KS  +IGVE D++G FPMWVSDGDRKLLQSSA+VRANLVVAKDGSGKFRTVQAAID
Sbjct: 181 EEAKKSETKIGVEDDRDGPFPMWVSDGDRKLLQSSARVRANLVVAKDGSGKFRTVQAAID 240

Query: 241 AAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSS 300
           AAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSS
Sbjct: 241 AAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSS 300

Query: 301 ATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360
           ATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF
Sbjct: 301 ATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQF 360

Query: 361 YKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420
           YKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI
Sbjct: 361 YKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQI 420

Query: 421 RAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQATLYYGE 480
           RAAADLRPM+GSVKTY+GRPWKKYSRTVIMRSYIDSLVSPAGWLAWQ SKFAQATLYYGE
Sbjct: 421 RAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYYGE 480

Query: 481 YRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           YRNIGPRA TR+RV+WPGFHVI+SPNVASKFTVQRLIAGQTWLPATGVPFKLG+
Sbjct: 481 YRNIGPRAFTRYRVRWPGFHVIKSPNVASKFTVQRLIAGQTWLPATGVPFKLGV 533

BLAST of Clc10G05890 vs. NCBI nr
Match: XP_008452338.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo] >XP_016901218.1 PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo])

HSP 1 Score: 935.3 bits (2416), Expect = 2.4e-268
Identity = 475/540 (87.96%), Postives = 498/540 (92.22%), Query Frame = 0

Query: 1   MASHYK-ILFFSIT-LFSILS-NISGNNTINKWCKTTPHPKVCISFMSPIKPPPLNRPEF 60
           MASHYK ILF SIT LF+ILS N+S NN I KWCKTTPHPKVCISFMSPIKPPP NR EF
Sbjct: 1   MASHYKIILFISITLLFTILSNNVSANNPIIKWCKTTPHPKVCISFMSPIKPPPQNREEF 60

Query: 61  RTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDG 120
           R +AI+T  EKA EA+ Y  RFGP+CKTTRQR AWTDC KLYNDVVFQLNRTLQC+++D 
Sbjct: 61  RQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDE 120

Query: 121 TIQRR-RCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAING 180
           TI RR  CTDFDAQTWLSSALTDIDLC SGAADLNVTDFIAPIKC NVSKMISNCLAING
Sbjct: 121 TIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAING 180

Query: 181 VFLKEGVKSVNRIGVEFDQ--NGSFPMWVSDGDRKLLQS-SAKVRANLVVAKDGSGKFRT 240
            FL+E        GV++D   NGSFPMWVS+GDRKLL+S   +VRANLVVAKDGSGKFR 
Sbjct: 181 GFLEEE-------GVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRR 240

Query: 241 VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAG 300
           VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGN+MLVGDGMRFTVITSGRSVAAG
Sbjct: 241 VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAG 300

Query: 301 FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV 360
           FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV
Sbjct: 301 FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV 360

Query: 361 LSQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGIS 420
           LSQRQFYKQCYIYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGIS
Sbjct: 361 LSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGIS 420

Query: 421 IHNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQA 480
           IHNSQIRAAADLRPM+GSVKTY+GRPWKKYSRTVIMRSYIDSLVSPAGWLAWQ SKFAQA
Sbjct: 421 IHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQA 480

Query: 481 TLYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           TLYYGEY NIGPRASTRFRVKWPGFHVI+SPNVASKF+V+RLIAGQTWLPATGVPFKLG+
Sbjct: 481 TLYYGEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 533

BLAST of Clc10G05890 vs. NCBI nr
Match: XP_011650542.1 (probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus] >KGN56178.1 hypothetical protein Csa_011446 [Cucumis sativus])

HSP 1 Score: 933.7 bits (2412), Expect = 7.0e-268
Identity = 464/536 (86.57%), Postives = 489/536 (91.23%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGNNTINKWCKTTPHPKVCISFMSPIKPPPLNRPEFRTM 60
           MASHYKILF SIT F++LSN    N I KWCKTTPHP+VC+S MSPIKPPP NR EFR M
Sbjct: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60

Query: 61  AIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQ 120
           AI+T  EKA EA+ Y  RFGP+CKT+RQR AWTDC KLYNDVV QLNRTL CV++D  I 
Sbjct: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120

Query: 121 RRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAINGVFLK 180
           RR CTDFDAQTWLSSALTDIDLC SGAADLNVTDFI PIKC NVSKMISNCLAING FL+
Sbjct: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180

Query: 181 EGVKSVNRIGVEFD--QNGSFPMWVSDGDRKLLQS-SAKVRANLVVAKDGSGKFRTVQAA 240
           E        GV++D  +NGSFPMWVS+GDRKLL+S   +VRANLVVAKDGSG FR VQAA
Sbjct: 181 EE-------GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAA 240

Query: 241 IDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 300
           IDAAARRRGRGRF+IYVKRGVYRENIEVGNDNGN+MLVGDGMRFTVITSGRSVAAGFTTF
Sbjct: 241 IDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTF 300

Query: 301 SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 360
           SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR
Sbjct: 301 SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 360

Query: 361 QFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 420
           QFYKQCY+YGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS
Sbjct: 361 QFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 420

Query: 421 QIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQATLYY 480
           QIRAAADLRPM+GSVKTY+GRPWKKYSRTVIMRSYID LVSPAGWLAWQ SKFAQATLYY
Sbjct: 421 QIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYY 480

Query: 481 GEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           GEYRNIGPRASTRFRVKWPGFHVI+SPNVASKF+VQRLIAGQTWLPATGVPFKLG+
Sbjct: 481 GEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

BLAST of Clc10G05890 vs. NCBI nr
Match: KAG7015521.1 (putative pectinesterase/pectinesterase inhibitor 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887.5 bits (2292), Expect = 5.7e-254
Identity = 445/539 (82.56%), Postives = 479/539 (88.87%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGN------NTINKWCKTTPHPKVCISFMSPIKPPPLNR 60
           MA H +I FFSI LF++LSN S +      NTINKWC TTPHPKVCI FMSP+K PP NR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  PEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVL 120
            EFR MAI+T  E A +AQNY +RFGP+CKTTRQRAAWTDC  LYND VFQLNRT+QC+ 
Sbjct: 61  LEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 120

Query: 121 SDGTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAI 180
            DG  + R CTDFDAQTWLSSALT+ID+C SGAADLNVTDFI PIKCQNVSKMISNCLAI
Sbjct: 121 -DGGNRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 180

Query: 181 NGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTV 240
           NG FL+E  KS   +G E  QNG FP WVS GDRKLLQ SA+VRA+LVVAKDGSGKFRTV
Sbjct: 181 NGRFLQEETKS-RTMGTEEYQNG-FPTWVSSGDRKLLQ-SARVRAHLVVAKDGSGKFRTV 240

Query: 241 QAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RFVIY+KRGVY+ENIEVGNDN N+MLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQAT 480
           HNSQIRAAADL+PMIGSVKTY+GRPWKKYSRTVIMRSYIDSLVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKFTV+RLIAG TWLPATGVPFKL L
Sbjct: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

BLAST of Clc10G05890 vs. NCBI nr
Match: XP_022932129.1 (probable pectinesterase/pectinesterase inhibitor 33 [Cucurbita moschata])

HSP 1 Score: 884.8 bits (2285), Expect = 3.7e-253
Identity = 444/539 (82.37%), Postives = 477/539 (88.50%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGN------NTINKWCKTTPHPKVCISFMSPIKPPPLNR 60
           MA H +I FFSI LF++LSN S +      NTINKWC TTPHPKVCI FMSP+K PP NR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  PEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVL 120
            EFR MAI+T  E A +AQNY + FGP+CKTTRQRAAWTDC  LYND VFQLNRT+QC+ 
Sbjct: 61  LEFRKMAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 120

Query: 121 SDGTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAI 180
            DG  + R CTDFDAQTWLSSALT+ID+C SGAADLNVTDFI PIKCQNVSKMISNCLAI
Sbjct: 121 -DGGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 180

Query: 181 NGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTV 240
           NG FL+E  KS    G E  QNG FP WVS GDRKLLQ SA+VRA+LVVAKDGSGKFRTV
Sbjct: 181 NGRFLQEETKS-RTTGTEEYQNG-FPTWVSSGDRKLLQ-SARVRAHLVVAKDGSGKFRTV 240

Query: 241 QAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RFVIY+KRGVY+ENIEVGNDN N+MLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQAT 480
           HNSQIRAAADL+PMIGSVKTY+GRPWKKYSRTVIMRSYIDSLVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKFTV+RLIAG TWLPATGVPFKL L
Sbjct: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

BLAST of Clc10G05890 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 6.8e-165
Identity = 296/528 (56.06%), Postives = 383/528 (72.54%), Query Frame = 0

Query: 16  SILSNISGNNTINKWCKTTPHPKVCISFMSPIKP------PPLNRPEFRTMAIRTAFEKA 75
           S ++N S +  +  WC  TPH + C  +            PP  R EF  M ++ A ++A
Sbjct: 73  SPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQA 132

Query: 76  VEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQRRRCTDFDA 135
           V   +   +FGPSC   +++AAW+DC+ L+ + V QLNRTL+  L+       +CTDFDA
Sbjct: 133 VITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLK-GLNPAASSDVKCTDFDA 192

Query: 136 QTWLSSALTDIDLCLSGAADLNVTDFIAP-IKCQNVSKMISNCLAINGVFLKEGVKSVNR 195
           QTWLS+A T+I+ C SG+ DLNV+DF+ P I  +N+S +I NCLA+NGV +K+   +   
Sbjct: 193 QTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTA 252

Query: 196 IGVEFDQNGSFPMWVSDGDRKLLQSS--AKVRANLVVAKDGSGKFRTVQAAIDAAARRRG 255
              E+     FP WVS  +R+LL S+  AK   +LVVA+D SG FR++QAAI+ AARRR 
Sbjct: 253 NHKEY-----FPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRF 312

Query: 256 RGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQG 315
           + RFVIYVK+GVYRENI+VGNDN N+MLVGDG R T+ITSGRSV  G+TT++SAT G  G
Sbjct: 313 KSRFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGG 372

Query: 316 PGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYIY 375
             F+A+D+ F+NTAGP  GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CYI 
Sbjct: 373 QRFVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYIS 432

Query: 376 GTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLR 435
           GTIDFIFGNAAVV QNCMI VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+
Sbjct: 433 GTIDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLK 492

Query: 436 PMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQK-SKFAQATLYYGEYRNIGP 495
           P+I + KTY+GRPW+ YSR  IM++YID+ +SP GW  W + S FA  T++YGEY+N GP
Sbjct: 493 PVIRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGP 552

Query: 496 RASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
            +STR+RV+W GFH I S  VAS+FTV  LIAG +WLPATGVPFK GL
Sbjct: 553 GSSTRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Clc10G05890 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.6e-164
Identity = 302/543 (55.62%), Postives = 381/543 (70.17%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSI-----LSNISGNNTINKWCKTTPHPKVCISFMSPIK--PPPLN 60
           M     ILF  + L  +     L+ ++  N+ + WC  TP+P  C  +         P  
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  RPEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCV 120
             EFR M +  A ++A+ A+   T  G +C  ++++A   DC+ LY D + QLNRTL  V
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 LSDGTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQ-NVSKMISNCL 180
            S      + CTDFDAQTWLS+ALT+ + C  G++DLNVTDFI PI     +S +ISNCL
Sbjct: 124 -SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCL 183

Query: 181 AINGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFR 240
           A+NG  L  G    N+     +Q G FP W+S  D++LL++   VRANLVVAKDGSG F 
Sbjct: 184 AVNGALLTAG----NKGNTTANQKG-FPTWLSRKDKRLLRA---VRANLVVAKDGSGHFN 243

Query: 241 TVQAAIDAAARRR-GRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVA 300
           TVQAAID A RR+   GRFVIYVKRG+Y+ENI V  +N ++MLVGDGMR T+IT GRSV 
Sbjct: 244 TVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQ 303

Query: 301 AGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTL 360
            G+TT++SATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTL
Sbjct: 304 GGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTL 363

Query: 361 MVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSG 420
           MV SQRQFY++CYIYGT+DFIFGNAA V QNC+I  RRPLKGQ NVITAQGR DPFQN+G
Sbjct: 364 MVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTG 423

Query: 421 ISIHNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAW-QKSKF 480
           ISIHNS+I  A DL+P++G+VKTYMGRPW K+SRTV++++Y+D++VSP GW  W + S F
Sbjct: 424 ISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVF 483

Query: 481 AQATLYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFK 534
              TL+Y EY+N GP +STR+RV W GFHV+   + AS FTV + IAG  WLP TG+PF 
Sbjct: 484 GLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFT 536

BLAST of Clc10G05890 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 7.5e-164
Identity = 308/535 (57.57%), Postives = 381/535 (71.21%), Query Frame = 0

Query: 3   SHYKILFFSITLFSILSNISGNNT-INKWCKTTPHPKVCISFMSPIKPPPLNRP----EF 62
           S + +L   + L  + +   GN+T I+ WC  TP+P  C  +   IK      P    EF
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF--IKHSGFRLPTQISEF 72

Query: 63  RTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDG 122
           R + +  A ++AV A +  T    +C   +++A   DC+ LY D V QLNRTLQ V S  
Sbjct: 73  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSK- 132

Query: 123 TIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQ-NVSKMISNCLAING 182
               RRCTDFDAQTWLS+ALT+ + C  G++DLNV+DF  PI     +S +ISNCLA+NG
Sbjct: 133 --TGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNG 192

Query: 183 VFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTVQA 242
             L  G K+ +  G   D  G FP WVS  +R+LLQ  + VRANLVVAKDGSG F+TVQA
Sbjct: 193 ALLTAG-KNDSTTG---DSKG-FPTWVSRKERRLLQLQS-VRANLVVAKDGSGHFKTVQA 252

Query: 243 AIDAAARRR-GRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFT 302
           AID A RR+   GRFVIYVKRG+Y+EN+ V  +N N+MLVGDGMR+T+IT GRSV  G+T
Sbjct: 253 AIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYT 312

Query: 303 TFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLS 362
           T+SSATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV S
Sbjct: 313 TYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHS 372

Query: 363 QRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIH 422
           QRQFY++CYIYGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIH
Sbjct: 373 QRQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIH 432

Query: 423 NSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQK-SKFAQAT 482
           NS I  A DL+P++ SVKTYMGRPW  YSRTV++++YIDS+VSP GW  W K S +   T
Sbjct: 433 NSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDT 492

Query: 483 LYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPF 530
           L+Y EY+NIGP +STR+RV+W GFHV+   + AS F+V + IAG  WLP +G+PF
Sbjct: 493 LFYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

BLAST of Clc10G05890 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 509.2 bits (1310), Expect = 5.6e-143
Identity = 274/537 (51.02%), Postives = 354/537 (65.92%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGNN--TINKWCKTTPHPKVCISFM--SPIKPPPLNRPE 60
           MA    I+ F I    + S +SG N   +  WC  TP+PK C  F+  +    P  +  E
Sbjct: 2   MAFRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESE 61

Query: 61  FRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSD 120
           F  ++++   ++A+ A+ +A   GP C+ TR++AAW DC+KLY+  V ++N T+      
Sbjct: 62  FLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD----- 121

Query: 121 GTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAING 180
                 +C+  DAQTWLS+ALT++D C +G  +L VTD + P+   NVS ++ N LAIN 
Sbjct: 122 ---PNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINK 181

Query: 181 VFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTVQA 240
           V         N    E D    FP WV  GDRKLLQSS   + N VVAKDGSG F+T++ 
Sbjct: 182 V-------PFNYTPPEKD---GFPSWVKPGDRKLLQSSTP-KDNAVVAKDGSGNFKTIKE 241

Query: 241 AIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTT 300
           AIDAA+   G GRFVIYVK+GVY EN+E+     N+ML GDG+  T+IT  +SV  G TT
Sbjct: 242 AIDAAS---GSGRFVIYVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTT 301

Query: 301 FSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQ 360
           F+SAT    G GFIAR I F NTAG    QAVALRS SDLSVF++CSFE YQDTL V S 
Sbjct: 302 FNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSN 361

Query: 361 RQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHN 420
           RQFY+ C +YGT+DFIFGNAA VLQNC I+ RRP + + N ITAQGR DP QN+GI IHN
Sbjct: 362 RQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHN 421

Query: 421 SQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQATLY 480
           S++ AA+DLRP++GS KTY+GRPW++YSRTV M++ +DSL+ P GWL W    FA  TL+
Sbjct: 422 SRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWD-GNFALKTLF 481

Query: 481 YGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           Y E++N GP AST  RV WPGF V+ S + ASKFTV   +AG +W+P++ VPF  GL
Sbjct: 482 YAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Clc10G05890 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.2e-138
Identity = 264/531 (49.72%), Postives = 347/531 (65.35%), Query Frame = 0

Query: 7   ILFFSITLFSILSNISGNN--TINKWCKTTPHPKVCISFMSPIKPPPLNR--PEFRTMAI 66
           ++  S+ LFS+     G +   +  WC  TP+P+ C  F++        +   +F  +++
Sbjct: 6   LITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 65

Query: 67  RTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQRR 126
           + A E+A  AQ+     G  C+  R++AAW DC +LY   V +LN+T     S G     
Sbjct: 66  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNS--SPG----- 125

Query: 127 RCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAINGVFLKEG 186
            CT  D QTWLS+ALT+++ C +   DL V +++ P+   NV+K+ISN L++N V   E 
Sbjct: 126 -CTKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVPYNEP 185

Query: 187 VKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTVQAAIDAAA 246
                           FP WV  GDRKLLQ++   RAN+VVA+DGSG  +T+Q A+ AAA
Sbjct: 186 -----------SYKDGFPTWVKPGDRKLLQTTP--RANIVVAQDGSGNVKTIQEAV-AAA 245

Query: 247 RRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATA 306
            R G  R+VIY+K G Y ENIEV     N+M VGDG+  T+IT  +SV  G TTF SAT 
Sbjct: 246 SRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATV 305

Query: 307 GIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQ 366
            + G  FIARDI   NTAGP   QAVALRS SDLSVF+RCSFEGYQDTL V SQRQFY++
Sbjct: 306 AVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRE 365

Query: 367 CYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAA 426
           C IYGT+DFIFGNAAVVLQNC I+ R+P   + N +TAQGR DP Q++GI IHN ++ AA
Sbjct: 366 CDIYGTVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAA 425

Query: 427 ADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQATLYYGEYRN 486
           +DL+P+  SVKT++GRPWK+YSRTV +++++DSL++PAGW+ W    FA  TLYY EY N
Sbjct: 426 SDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEW-SGDFALNTLYYAEYMN 485

Query: 487 IGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
            GP +ST  RVKW G+HV+ SP+  S+FTV   IAG +WLPAT VPF  GL
Sbjct: 486 TGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Clc10G05890 vs. ExPASy TrEMBL
Match: A0A1S3BUR4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.2e-268
Identity = 475/540 (87.96%), Postives = 498/540 (92.22%), Query Frame = 0

Query: 1   MASHYK-ILFFSIT-LFSILS-NISGNNTINKWCKTTPHPKVCISFMSPIKPPPLNRPEF 60
           MASHYK ILF SIT LF+ILS N+S NN I KWCKTTPHPKVCISFMSPIKPPP NR EF
Sbjct: 1   MASHYKIILFISITLLFTILSNNVSANNPIIKWCKTTPHPKVCISFMSPIKPPPQNREEF 60

Query: 61  RTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDG 120
           R +AI+T  EKA EA+ Y  RFGP+CKTTRQR AWTDC KLYNDVVFQLNRTLQC+++D 
Sbjct: 61  RQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDE 120

Query: 121 TIQRR-RCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAING 180
           TI RR  CTDFDAQTWLSSALTDIDLC SGAADLNVTDFIAPIKC NVSKMISNCLAING
Sbjct: 121 TIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAING 180

Query: 181 VFLKEGVKSVNRIGVEFDQ--NGSFPMWVSDGDRKLLQS-SAKVRANLVVAKDGSGKFRT 240
            FL+E        GV++D   NGSFPMWVS+GDRKLL+S   +VRANLVVAKDGSGKFR 
Sbjct: 181 GFLEEE-------GVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRR 240

Query: 241 VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAG 300
           VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGN+MLVGDGMRFTVITSGRSVAAG
Sbjct: 241 VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAG 300

Query: 301 FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV 360
           FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV
Sbjct: 301 FTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMV 360

Query: 361 LSQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGIS 420
           LSQRQFYKQCYIYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGIS
Sbjct: 361 LSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGIS 420

Query: 421 IHNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQA 480
           IHNSQIRAAADLRPM+GSVKTY+GRPWKKYSRTVIMRSYIDSLVSPAGWLAWQ SKFAQA
Sbjct: 421 IHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQA 480

Query: 481 TLYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           TLYYGEY NIGPRASTRFRVKWPGFHVI+SPNVASKF+V+RLIAGQTWLPATGVPFKLG+
Sbjct: 481 TLYYGEYHNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 533

BLAST of Clc10G05890 vs. ExPASy TrEMBL
Match: A0A0A0L330 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.4e-268
Identity = 464/536 (86.57%), Postives = 489/536 (91.23%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGNNTINKWCKTTPHPKVCISFMSPIKPPPLNRPEFRTM 60
           MASHYKILF SIT F++LSN    N I KWCKTTPHP+VC+S MSPIKPPP NR EFR M
Sbjct: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60

Query: 61  AIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQ 120
           AI+T  EKA EA+ Y  RFGP+CKT+RQR AWTDC KLYNDVV QLNRTL CV++D  I 
Sbjct: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120

Query: 121 RRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAINGVFLK 180
           RR CTDFDAQTWLSSALTDIDLC SGAADLNVTDFI PIKC NVSKMISNCLAING FL+
Sbjct: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180

Query: 181 EGVKSVNRIGVEFD--QNGSFPMWVSDGDRKLLQS-SAKVRANLVVAKDGSGKFRTVQAA 240
           E        GV++D  +NGSFPMWVS+GDRKLL+S   +VRANLVVAKDGSG FR VQAA
Sbjct: 181 EE-------GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAA 240

Query: 241 IDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTF 300
           IDAAARRRGRGRF+IYVKRGVYRENIEVGNDNGN+MLVGDGMRFTVITSGRSVAAGFTTF
Sbjct: 241 IDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTF 300

Query: 301 SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 360
           SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR
Sbjct: 301 SSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQR 360

Query: 361 QFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 420
           QFYKQCY+YGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS
Sbjct: 361 QFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNS 420

Query: 421 QIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQATLYY 480
           QIRAAADLRPM+GSVKTY+GRPWKKYSRTVIMRSYID LVSPAGWLAWQ SKFAQATLYY
Sbjct: 421 QIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYY 480

Query: 481 GEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           GEYRNIGPRASTRFRVKWPGFHVI+SPNVASKF+VQRLIAGQTWLPATGVPFKLG+
Sbjct: 481 GEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

BLAST of Clc10G05890 vs. ExPASy TrEMBL
Match: A0A6J1EW59 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.8e-253
Identity = 444/539 (82.37%), Postives = 477/539 (88.50%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGN------NTINKWCKTTPHPKVCISFMSPIKPPPLNR 60
           MA H +I FFSI LF++LSN S +      NTINKWC TTPHPKVCI FMSP+K PP NR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  PEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVL 120
            EFR MAI+T  E A +AQNY + FGP+CKTTRQRAAWTDC  LYND VFQLNRT+QC+ 
Sbjct: 61  LEFRKMAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 120

Query: 121 SDGTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAI 180
            DG  + R CTDFDAQTWLSSALT+ID+C SGAADLNVTDFI PIKCQNVSKMISNCLAI
Sbjct: 121 -DGGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 180

Query: 181 NGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTV 240
           NG FL+E  KS    G E  QNG FP WVS GDRKLLQ SA+VRA+LVVAKDGSGKFRTV
Sbjct: 181 NGRFLQEETKS-RTTGTEEYQNG-FPTWVSSGDRKLLQ-SARVRAHLVVAKDGSGKFRTV 240

Query: 241 QAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RFVIY+KRGVY+ENIEVGNDN N+MLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQAT 480
           HNSQIRAAADL+PMIGSVKTY+GRPWKKYSRTVIMRSYIDSLVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKFTV+RLIAG TWLPATGVPFKL L
Sbjct: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

BLAST of Clc10G05890 vs. ExPASy TrEMBL
Match: A0A6J1JCM7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.6e-252
Identity = 443/539 (82.19%), Postives = 476/539 (88.31%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGN------NTINKWCKTTPHPKVCISFMSPIKPPPLNR 60
           MA H +I FFSI LF++LSN S +      NTINKWC TTPHPKVCI FMSP+K PP NR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  PEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVL 120
            EFR MAI+T  E A +AQNY +RFGP+CKTTRQRAAWTDC  LYND VFQLNRT++C L
Sbjct: 61  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRC-L 120

Query: 121 SDGTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAI 180
             GT + R CTDFDAQTWLSSALT+ID+C SGAADLNVTDFI PIKCQNVS+MISNCLAI
Sbjct: 121 HAGT-RLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAI 180

Query: 181 NGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTV 240
           NG FL EG       G E  QNG FP WVS GDRKLLQ SA+VRA+LVVAKDGSGKFRTV
Sbjct: 181 NGRFL-EGETKSRTTGAEEYQNG-FPRWVSSGDRKLLQ-SARVRAHLVVAKDGSGKFRTV 240

Query: 241 QAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RFVIY+KRGVYRENIE+GNDN N+MLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGF 300

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQAT 480
           HNSQIRAAADL+PMIGSVKTY+GRPWKKYSRTVIMRSYIDSLVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKFTV+RLIAG TWLPATGVPFKL L
Sbjct: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKLDL 534

BLAST of Clc10G05890 vs. ExPASy TrEMBL
Match: A0A5A7TVK4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 8.1e-246
Identity = 433/494 (87.65%), Postives = 455/494 (92.11%), Query Frame = 0

Query: 44  MSPIKPPPLNRPEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVV 103
           MSPIKPPP NR EFR +AI+T  EKA EA+ Y  RFGP+CKTTRQR AWTDC KLYNDVV
Sbjct: 1   MSPIKPPPQNREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVV 60

Query: 104 FQLNRTLQCVLSDGTIQRR-RCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQ 163
           FQLNRTLQC+++D TI RR  CTDFDAQTWLSSALTDIDLC SGAADLNVTDFIAPIKC 
Sbjct: 61  FQLNRTLQCLVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCL 120

Query: 164 NVSKMISNCLAINGVFLKEGVKSVNRIGVEFDQ--NGSFPMWVSDGDRKLLQS-SAKVRA 223
           NVSKMISNCLAING F++E        GV++D   NGSFPMWVS+GDRKLL+S   +VRA
Sbjct: 121 NVSKMISNCLAINGGFIEEE-------GVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRA 180

Query: 224 NLVVAKDGSGKFRTVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGM 283
           NLVVAKDGSGKFR VQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGN+MLVGDGM
Sbjct: 181 NLVVAKDGSGKFRRVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGM 240

Query: 284 RFTVITSGRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVF 343
           RFTVITSGRSVAAGFTTFS     IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVF
Sbjct: 241 RFTVITSGRSVAAGFTTFS-----IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVF 300

Query: 344 HRCSFEGYQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVIT 403
           HRCSFEGYQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVIT
Sbjct: 301 HRCSFEGYQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVIT 360

Query: 404 AQGREDPFQNSGISIHNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSP 463
           AQGREDPFQNSGISIHNSQIRAAADLRPM+GSVKTY+GRPWKKYSRTVIMRSYIDSLVSP
Sbjct: 361 AQGREDPFQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSP 420

Query: 464 AGWLAWQKSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQ 523
           AGWLAWQ SKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVI+SPNVASKF+V+RLIAGQ
Sbjct: 421 AGWLAWQSSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQ 480

Query: 524 TWLPATGVPFKLGL 534
           TWLPATGVPFKLG+
Sbjct: 481 TWLPATGVPFKLGV 482

BLAST of Clc10G05890 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 582.0 bits (1499), Expect = 4.8e-166
Identity = 296/528 (56.06%), Postives = 383/528 (72.54%), Query Frame = 0

Query: 16  SILSNISGNNTINKWCKTTPHPKVCISFMSPIKP------PPLNRPEFRTMAIRTAFEKA 75
           S ++N S +  +  WC  TPH + C  +            PP  R EF  M ++ A ++A
Sbjct: 73  SPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQA 132

Query: 76  VEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQRRRCTDFDA 135
           V   +   +FGPSC   +++AAW+DC+ L+ + V QLNRTL+  L+       +CTDFDA
Sbjct: 133 VITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLK-GLNPAASSDVKCTDFDA 192

Query: 136 QTWLSSALTDIDLCLSGAADLNVTDFIAP-IKCQNVSKMISNCLAINGVFLKEGVKSVNR 195
           QTWLS+A T+I+ C SG+ DLNV+DF+ P I  +N+S +I NCLA+NGV +K+   +   
Sbjct: 193 QTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTA 252

Query: 196 IGVEFDQNGSFPMWVSDGDRKLLQSS--AKVRANLVVAKDGSGKFRTVQAAIDAAARRRG 255
              E+     FP WVS  +R+LL S+  AK   +LVVA+D SG FR++QAAI+ AARRR 
Sbjct: 253 NHKEY-----FPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRF 312

Query: 256 RGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQG 315
           + RFVIYVK+GVYRENI+VGNDN N+MLVGDG R T+ITSGRSV  G+TT++SAT G  G
Sbjct: 313 KSRFVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGG 372

Query: 316 PGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYIY 375
             F+A+D+ F+NTAGP  GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CYI 
Sbjct: 373 QRFVAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYIS 432

Query: 376 GTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLR 435
           GTIDFIFGNAAVV QNCMI VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+
Sbjct: 433 GTIDFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLK 492

Query: 436 PMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQK-SKFAQATLYYGEYRNIGP 495
           P+I + KTY+GRPW+ YSR  IM++YID+ +SP GW  W + S FA  T++YGEY+N GP
Sbjct: 493 PVIRAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGP 552

Query: 496 RASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
            +STR+RV+W GFH I S  VAS+FTV  LIAG +WLPATGVPFK GL
Sbjct: 553 GSSTRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Clc10G05890 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 580.1 bits (1494), Expect = 1.8e-165
Identity = 302/543 (55.62%), Postives = 381/543 (70.17%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSI-----LSNISGNNTINKWCKTTPHPKVCISFMSPIK--PPPLN 60
           M     ILF  + L  +     L+ ++  N+ + WC  TP+P  C  +         P  
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  RPEFRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCV 120
             EFR M +  A ++A+ A+   T  G +C  ++++A   DC+ LY D + QLNRTL  V
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 LSDGTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQ-NVSKMISNCL 180
            S      + CTDFDAQTWLS+ALT+ + C  G++DLNVTDFI PI     +S +ISNCL
Sbjct: 124 -SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCL 183

Query: 181 AINGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFR 240
           A+NG  L  G    N+     +Q G FP W+S  D++LL++   VRANLVVAKDGSG F 
Sbjct: 184 AVNGALLTAG----NKGNTTANQKG-FPTWLSRKDKRLLRA---VRANLVVAKDGSGHFN 243

Query: 241 TVQAAIDAAARRR-GRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVA 300
           TVQAAID A RR+   GRFVIYVKRG+Y+ENI V  +N ++MLVGDGMR T+IT GRSV 
Sbjct: 244 TVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQ 303

Query: 301 AGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTL 360
            G+TT++SATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTL
Sbjct: 304 GGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTL 363

Query: 361 MVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSG 420
           MV SQRQFY++CYIYGT+DFIFGNAA V QNC+I  RRPLKGQ NVITAQGR DPFQN+G
Sbjct: 364 MVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTG 423

Query: 421 ISIHNSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAW-QKSKF 480
           ISIHNS+I  A DL+P++G+VKTYMGRPW K+SRTV++++Y+D++VSP GW  W + S F
Sbjct: 424 ISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVF 483

Query: 481 AQATLYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFK 534
              TL+Y EY+N GP +STR+RV W GFHV+   + AS FTV + IAG  WLP TG+PF 
Sbjct: 484 GLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFT 536

BLAST of Clc10G05890 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 578.6 bits (1490), Expect = 5.3e-165
Identity = 308/535 (57.57%), Postives = 381/535 (71.21%), Query Frame = 0

Query: 3   SHYKILFFSITLFSILSNISGNNT-INKWCKTTPHPKVCISFMSPIKPPPLNRP----EF 62
           S + +L   + L  + +   GN+T I+ WC  TP+P  C  +   IK      P    EF
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF--IKHSGFRLPTQISEF 72

Query: 63  RTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSDG 122
           R + +  A ++AV A +  T    +C   +++A   DC+ LY D V QLNRTLQ V S  
Sbjct: 73  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSK- 132

Query: 123 TIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQ-NVSKMISNCLAING 182
               RRCTDFDAQTWLS+ALT+ + C  G++DLNV+DF  PI     +S +ISNCLA+NG
Sbjct: 133 --TGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNG 192

Query: 183 VFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTVQA 242
             L  G K+ +  G   D  G FP WVS  +R+LLQ  + VRANLVVAKDGSG F+TVQA
Sbjct: 193 ALLTAG-KNDSTTG---DSKG-FPTWVSRKERRLLQLQS-VRANLVVAKDGSGHFKTVQA 252

Query: 243 AIDAAARRR-GRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFT 302
           AID A RR+   GRFVIYVKRG+Y+EN+ V  +N N+MLVGDGMR+T+IT GRSV  G+T
Sbjct: 253 AIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYT 312

Query: 303 TFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLS 362
           T+SSATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV S
Sbjct: 313 TYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHS 372

Query: 363 QRQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIH 422
           QRQFY++CYIYGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIH
Sbjct: 373 QRQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIH 432

Query: 423 NSQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQK-SKFAQAT 482
           NS I  A DL+P++ SVKTYMGRPW  YSRTV++++YIDS+VSP GW  W K S +   T
Sbjct: 433 NSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDT 492

Query: 483 LYYGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPF 530
           L+Y EY+NIGP +STR+RV+W GFHV+   + AS F+V + IAG  WLP +G+PF
Sbjct: 493 LFYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

BLAST of Clc10G05890 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 509.2 bits (1310), Expect = 4.0e-144
Identity = 274/537 (51.02%), Postives = 354/537 (65.92%), Query Frame = 0

Query: 1   MASHYKILFFSITLFSILSNISGNN--TINKWCKTTPHPKVCISFM--SPIKPPPLNRPE 60
           MA    I+ F I    + S +SG N   +  WC  TP+PK C  F+  +    P  +  E
Sbjct: 2   MAFRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESE 61

Query: 61  FRTMAIRTAFEKAVEAQNYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVLSD 120
           F  ++++   ++A+ A+ +A   GP C+ TR++AAW DC+KLY+  V ++N T+      
Sbjct: 62  FLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD----- 121

Query: 121 GTIQRRRCTDFDAQTWLSSALTDIDLCLSGAADLNVTDFIAPIKCQNVSKMISNCLAING 180
                 +C+  DAQTWLS+ALT++D C +G  +L VTD + P+   NVS ++ N LAIN 
Sbjct: 122 ---PNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINK 181

Query: 181 VFLKEGVKSVNRIGVEFDQNGSFPMWVSDGDRKLLQSSAKVRANLVVAKDGSGKFRTVQA 240
           V         N    E D    FP WV  GDRKLLQSS   + N VVAKDGSG F+T++ 
Sbjct: 182 V-------PFNYTPPEKD---GFPSWVKPGDRKLLQSSTP-KDNAVVAKDGSGNFKTIKE 241

Query: 241 AIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTT 300
           AIDAA+   G GRFVIYVK+GVY EN+E+     N+ML GDG+  T+IT  +SV  G TT
Sbjct: 242 AIDAAS---GSGRFVIYVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTT 301

Query: 301 FSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQ 360
           F+SAT    G GFIAR I F NTAG    QAVALRS SDLSVF++CSFE YQDTL V S 
Sbjct: 302 FNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSN 361

Query: 361 RQFYKQCYIYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHN 420
           RQFY+ C +YGT+DFIFGNAA VLQNC I+ RRP + + N ITAQGR DP QN+GI IHN
Sbjct: 362 RQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHN 421

Query: 421 SQIRAAADLRPMIGSVKTYMGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQKSKFAQATLY 480
           S++ AA+DLRP++GS KTY+GRPW++YSRTV M++ +DSL+ P GWL W    FA  TL+
Sbjct: 422 SRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWD-GNFALKTLF 481

Query: 481 YGEYRNIGPRASTRFRVKWPGFHVIRSPNVASKFTVQRLIAGQTWLPATGVPFKLGL 534
           Y E++N GP AST  RV WPGF V+ S + ASKFTV   +AG +W+P++ VPF  GL
Sbjct: 482 YAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Clc10G05890 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 391.7 bits (1005), Expect = 9.2e-109
Identity = 217/508 (42.72%), Postives = 311/508 (61.22%), Query Frame = 0

Query: 28  NKWCKTTPHPKVCISFMSPIKPPPL-NRPEFRTMAIRTAFEKAVEAQNYATRFGPSCKTT 87
           N+ C  TPHP  C + +  IK  P+ +  +F  +++    + AV+A++      P   + 
Sbjct: 5   NEMCDETPHPGECKTLL--IKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS- 64

Query: 88  RQRAAWTDCLKLYNDVVFQLNRTLQCVLSDGTIQRRRCTDFDAQTWLSSALTDIDLCLSG 147
             + AW +C+ LY   + +LN ++ C        +  C+  D Q WLS+ALT++D C   
Sbjct: 65  --KQAWEECMDLYEQTIHRLNESVLC-------PKNVCSRSDVQAWLSTALTNLDTCQEE 124

Query: 148 AADLNVTDFIAPIKCQNVSKMISNCLAINGVFLKEGVKSVNRIGVEFDQNGSFPMWVSDG 207
            ++L V+        ++++  + N LAIN        K + + G EF         +S  
Sbjct: 125 MSELGVSSH----SLESITIDVINTLAIN--------KRMEQNGKEFG--------ISKI 184

Query: 208 DRKLLQSSAKVRANLVVAKDGSGKFRTVQAAIDAAARR-RGRGRFVIYVKRGVYRENIEV 267
             K L    KV  ++VVA+DGSG ++T+Q A++ A  R +G  R+VI+VK+GVY E + V
Sbjct: 185 TMKTLSIGEKV--DVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNV 244

Query: 268 GNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMG 327
           G  + N+M+ GDG+  T+IT  +S   GF+T+ SAT   +G GF+ RDI   NTAGP   
Sbjct: 245 GIKSNNIMITGDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENH 304

Query: 328 QAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCMI 387
           QAVALRS+SD+SVF+RCS EGYQDTL V S RQF+++C IYGT+DFIFGNAA VLQNC I
Sbjct: 305 QAVALRSNSDMSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRI 364

Query: 388 YVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMIGSVKTYMGRPWKKYSR 447
           + R P  G VN ITAQ R +P Q +GI IHNS ++ A  ++  +G VKTY+GRPW+ Y+R
Sbjct: 365 FARNPPNG-VNTITAQSRFNPNQTTGIVIHNSVVKGAPGVQ--LGGVKTYLGRPWRSYAR 424

Query: 448 TVIMRSYIDSLVSPAGWLAWQKSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIRSPN 507
           TV++ +Y+D+L+ P GW+ W  +  A +TLYYGEY+N GP + T  RV W GFHVI    
Sbjct: 425 TVVIGTYLDTLIEPNGWIDWD-NVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQ 474

Query: 508 VASKFTVQRLIAGQTWLPATGVPFKLGL 534
            A +FT+ + I   +WLP T VPF + L
Sbjct: 485 EAREFTLPKFIDSASWLPPTKVPFTINL 474