Cla97C11G221577 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C11G221577
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	EF hand calcium-binding family protein
Location	Cla97Chr11: 27558065 .. 27558541 (+)
RNA-Seq Expression	Cla97C11G221577
Synteny	Cla97C11G221577

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAAGCCGGATCTCTCTCGGCCGACACCGAGACTCTGTGCCACATCTTTAGCCTAATAGAAGCGTTTCGGGCATTCGATGCAGACAACGACGGGTTGATCTCATCAGCCGAGGTCGAGGGAATCATGGGGTCGCTCGGGTACAATATGAGTGAGCAAGACGTGAACATGATGATGGAGGAAGGCGACGTCGACAAAGACGGGTTGCTAAGCATTGGAGAGTTTTTGGAAATGAATGCAAAGAATATGGAGATTGGGGAGATGTTAGGGAGCTACTTGAAGATTGCTTTTGAGGCTCTAAAGGCAGATGGAGATGATCTTGTGAGTGGGGAGGAGCTTTATGATGTGTTTGTGAATTTAGGGTTGGAAGTTCCACTTGAGGATTGCATGGCTATTGTGGCTTCTATGGATGGAGATGGAGATGGAGCTATGTTTCTTCATGATTTTAAACTTATTGTTAATTCCCTTCACTAA

mRNA sequence

ATGGCTCAAGCCGGATCTCTCTCGGCCGACACCGAGACTCTGTGCCACATCTTTAGCCTAATAGAAGCGTTTCGGGCATTCGATGCAGACAACGACGGGTTGATCTCATCAGCCGAGGTCGAGGGAATCATGGGGTCGCTCGGGTACAATATGAGTGAGCAAGACGTGAACATGATGATGGAGGAAGGCGACGTCGACAAAGACGGGTTGCTAAGCATTGGAGAGTTTTTGGAAATGAATGCAAAGAATATGGAGATTGGGGAGATGTTAGGGAGCTACTTGAAGATTGCTTTTGAGGCTCTAAAGGCAGATGGAGATGATCTTGTGAGTGGGGAGGAGCTTTATGATGTGTTTGTGAATTTAGGGTTGGAAGTTCCACTTGAGGATTGCATGGCTATTGTGGCTTCTATGGATGGAGATGGAGATGGAGCTATGTTTCTTCATGATTTTAAACTTATTGTTAATTCCCTTCACTAA

Coding sequence (CDS)

ATGGCTCAAGCCGGATCTCTCTCGGCCGACACCGAGACTCTGTGCCACATCTTTAGCCTAATAGAAGCGTTTCGGGCATTCGATGCAGACAACGACGGGTTGATCTCATCAGCCGAGGTCGAGGGAATCATGGGGTCGCTCGGGTACAATATGAGTGAGCAAGACGTGAACATGATGATGGAGGAAGGCGACGTCGACAAAGACGGGTTGCTAAGCATTGGAGAGTTTTTGGAAATGAATGCAAAGAATATGGAGATTGGGGAGATGTTAGGGAGCTACTTGAAGATTGCTTTTGAGGCTCTAAAGGCAGATGGAGATGATCTTGTGAGTGGGGAGGAGCTTTATGATGTGTTTGTGAATTTAGGGTTGGAAGTTCCACTTGAGGATTGCATGGCTATTGTGGCTTCTATGGATGGAGATGGAGATGGAGCTATGTTTCTTCATGATTTTAAACTTATTGTTAATTCCCTTCACTAA

Protein sequence

MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH

Homology

[Close]

BLAST of Cla97C11G221577 vs. NCBI nr
Match: XP_038897482.1 (probable calcium-binding protein CML29 [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 1.0e-73
Identity = 147/158 (93.04%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSLSA+TETL HIF+LIEAFRAFDADNDGLISSAEV GIMGSLGY+M+EQDVNMMM
Sbjct: 1   MAQAGSLSAETETLSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYDMNEQDVNMMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGDVD+DGLLSIGEFLEMNAKNME+GE LGSYLKIAFEALKADGDDLVSGEELYDVFVN
Sbjct: 61  EEGDVDRDGLLSIGEFLEMNAKNMEVGE-LGSYLKIAFEALKADGDDLVSGEELYDVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           LGLEV LEDCMAIVASMDGDGDGA+FLHDFKLIVNSLH
Sbjct: 121 LGLEVSLEDCMAIVASMDGDGDGAVFLHDFKLIVNSLH 157

[Close]

BLAST of Cla97C11G221577 vs. NCBI nr
Match: XP_008444124.2 (PREDICTED: probable calcium-binding protein CML29 [Cucumis melo] >XP_016899854.1 PREDICTED: probable calcium-binding protein CML29 [Cucumis melo] >KAA0052396.1 putative calcium-binding protein CML29 [Cucumis melo var. makuwa] >TYK19222.1 putative calcium-binding protein CML29 [Cucumis melo var. makuwa])

HSP 1 Score: 271.2 bits (692), Expect = 5.8e-69
Identity = 139/158 (87.97%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSL  DTE L HIF+LIEAFRAFDADNDGLISSAEV GI+GSLGYN+SE+DV MMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGD DKDGLLS+GEFLEMNAKNME+GE LGSYLKIA EALKADGDDLVSGEELYDVFVN
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGE-LGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           LGL+V LED MAIVAS+DGDGDGAMFLHDFKLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Cla97C11G221577 vs. NCBI nr
Match: XP_004142616.1 (probable calcium-binding protein CML29 [Cucumis sativus] >KGN54599.1 hypothetical protein Csa_012056 [Cucumis sativus])

HSP 1 Score: 270.4 bits (690), Expect = 9.8e-69
Identity = 139/158 (87.97%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSL  DTE L HIF+LIEAFRAFDADNDGLISSAEV GIMGSLGYN+SE+DVNMMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYNLSEEDVNMMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGD DKDGLLS+GEFLEMNAKNM++GE LGSYLKIA EALKAD DDLVSGEELYDVFVN
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMDVGE-LGSYLKIALEALKADEDDLVSGEELYDVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           LGL+V LED MAIVAS+DGDGDGAMFLHDFKLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASIDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Cla97C11G221577 vs. NCBI nr
Match: XP_022142920.1 (probable calcium-binding protein CML29 [Momordica charantia])

HSP 1 Score: 262.3 bits (669), Expect = 2.7e-66
Identity = 129/158 (81.65%), Postives = 150/158 (94.94%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSLSA+TE+L H+FSLIEAFRAFDADNDGLI++ EV GIMGSLGYN+SE+DVN+MM
Sbjct: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGDVDKDGLLS+GEFLEMNA+++E+GE++GSYLKIA+EALKADGDDLVSGEELYDVF+N
Sbjct: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           +GLEV +EDC AIVASMDGD DGA+FL D KLIV+SLH
Sbjct: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 158

[Close]

BLAST of Cla97C11G221577 vs. NCBI nr
Match: XP_022981068.1 (probable calcium-binding protein CML29 [Cucurbita maxima])

HSP 1 Score: 256.1 bits (653), Expect = 1.9e-64
Identity = 129/157 (82.17%), Postives = 147/157 (93.63%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQ  SLSA+T++L HIFSLIEAFRAFDADNDGLIS+AEV GIMGSLGYN+SEQDVN+MM
Sbjct: 1   MAQEASLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGDVD+DGLLS+GEFLEM+A+++E+GE +G YLK+AFEAL ADGDDLVSGEELY+VFVN
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGE-VGRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSL 158
           LGL+V LEDCMAIVASMDGDGDGA+FL D KLIVNSL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Cla97C11G221577 vs. ExPASy Swiss-Prot
Match: Q5Z676 (Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 GN=CML29 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.2e-26
Identity = 69/158 (43.67%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 3   QAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEE 62
           QA  LS D E L +I SL+EAF+AFD+DNDGL+++ E+ G++ SLG +  E +V  M+  
Sbjct: 15  QAQQLSVDFEALSYISSLVEAFQAFDSDNDGLVTAPELRGLLASLGLDKPEHEVRDMLAR 74

Query: 63  GDVDKDGLLSIGEFLE-MNAKNMEIGEMLGSYLKIAFEALK--ADGDDLVSGEELYDVFV 122
            D D+DG LS+ E L+ MNA  + +G  LG+ L+ A  AL+  A  D ++  +EL  +  
Sbjct: 75  ADADRDGKLSVEELLDVMNAGQLGLG-ALGALLQSAVPALESAAGPDGVLGADELARLLS 134

Query: 123 NLGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSL 158
            +G    +EDCM I+A MDGDGDGA+ + +F+L+   L
Sbjct: 135 VMG-TASVEDCMEIIACMDGDGDGAISVEEFRLMAQLL 170

[Close]

BLAST of Cla97C11G221577 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 50/138 (36.23%), Postives = 82/138 (59.42%), Query Frame = 0


Query: 17  IFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEF 76
           I  L   F+ FD + DG ISS E+  IM SLG+ + E+++   + E D   DG ++  EF
Sbjct: 35  IRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEF 94

Query: 77  LEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVAS 136
           +E+N K M+  ++L + LK AF     DG+  +S EEL++V  +LG E  + +C  ++  
Sbjct: 95  VELNTKGMDQNDVLEN-LKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 154

Query: 137 MDGDGDGAMFLHDFKLIV 155
           +D DGDG +   +FK+++
Sbjct: 155 VDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Cla97C11G221577 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-16
Identity = 47/135 (34.81%), Postives = 75/135 (55.56%), Query Frame = 0


Query: 24  FRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEFLEMNAKN 83
           F  FDA+ DG IS  E+ G++ +LG N S++++  +MEE D DKDG +++ EF       
Sbjct: 25  FNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFAAFVKAE 84

Query: 84  MEIGEMLG--SYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVASMDGDG 143
            +     G  + LK AFE    D + L+S  EL+ +   LG      DC+ ++ S+D DG
Sbjct: 85  TDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIKSVDSDG 144

Query: 144 DGAMFLHDFKLIVNS 157
           DG +   +FK ++ +
Sbjct: 145 DGYVSFEEFKKMMTN 159

[Close]

BLAST of Cla97C11G221577 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-16
Identity = 47/140 (33.57%), Postives = 80/140 (57.14%), Query Frame = 0


Query: 17  IFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEF 76
           +  L E F+ FDA+ DG IS +E+  I+ SLG ++ E +V  MMEE D D DG +S+ EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 77  LEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVAS 136
           +++N K   + +     LK AF+    D +  +S  EL     ++G    +E+   I+ +
Sbjct: 84  VDLNNKGASVKD-----LKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHN 143

Query: 137 MDGDGDGAMFLHDFKLIVNS 157
           +D +GDG + + +F+ ++ S
Sbjct: 144 VDKNGDGLISVEEFQTMMTS 158

[Close]

BLAST of Cla97C11G221577 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.5e-16
Identity = 46/140 (32.86%), Postives = 80/140 (57.14%), Query Frame = 0


Query: 17  IFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEF 76
           +  L E F+ FDA+ DG IS +E+  I+ S+G  + E +V  MMEE D D DG +S+ EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEF 83

Query: 77  LEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVAS 136
           +++N K   + +     LK AF+    D +  +S  EL +   ++G    +E+   I+ +
Sbjct: 84  VDLNIKGATVKD-----LKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHN 143

Query: 137 MDGDGDGAMFLHDFKLIVNS 157
           +D +GDG + + +F+ ++ S
Sbjct: 144 VDKNGDGLINVEEFQTMMTS 158

[Close]

BLAST of Cla97C11G221577 vs. ExPASy TrEMBL
Match: A0A1S3B9P7 (probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.8e-69
Identity = 139/158 (87.97%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSL  DTE L HIF+LIEAFRAFDADNDGLISSAEV GI+GSLGYN+SE+DV MMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGD DKDGLLS+GEFLEMNAKNME+GE LGSYLKIA EALKADGDDLVSGEELYDVFVN
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGE-LGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           LGL+V LED MAIVAS+DGDGDGAMFLHDFKLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Cla97C11G221577 vs. ExPASy TrEMBL
Match: A0A5D3D6S0 (Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00180 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.8e-69
Identity = 139/158 (87.97%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSL  DTE L HIF+LIEAFRAFDADNDGLISSAEV GI+GSLGYN+SE+DV MMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGD DKDGLLS+GEFLEMNAKNME+GE LGSYLKIA EALKADGDDLVSGEELYDVFVN
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGE-LGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           LGL+V LED MAIVAS+DGDGDGAMFLHDFKLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Cla97C11G221577 vs. ExPASy TrEMBL
Match: A0A0A0L0M7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G376140 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.8e-69
Identity = 139/158 (87.97%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSL  DTE L HIF+LIEAFRAFDADNDGLISSAEV GIMGSLGYN+SE+DVNMMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYNLSEEDVNMMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGD DKDGLLS+GEFLEMNAKNM++GE LGSYLKIA EALKAD DDLVSGEELYDVFVN
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMDVGE-LGSYLKIALEALKADEDDLVSGEELYDVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           LGL+V LED MAIVAS+DGDGDGAMFLHDFKLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASIDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Cla97C11G221577 vs. ExPASy TrEMBL
Match: A0A6J1CMA3 (probable calcium-binding protein CML29 OS=Momordica charantia OX=3673 GN=LOC111012911 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-66
Identity = 129/158 (81.65%), Postives = 150/158 (94.94%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQAGSLSA+TE+L H+FSLIEAFRAFDADNDGLI++ EV GIMGSLGYN+SE+DVN+MM
Sbjct: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGDVDKDGLLS+GEFLEMNA+++E+GE++GSYLKIA+EALKADGDDLVSGEELYDVF+N
Sbjct: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSLH 159
           +GLEV +EDC AIVASMDGD DGA+FL D KLIV+SLH
Sbjct: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 158

[Close]

BLAST of Cla97C11G221577 vs. ExPASy TrEMBL
Match: A0A6J1J135 (probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC111480326 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 9.3e-65
Identity = 129/157 (82.17%), Postives = 147/157 (93.63%), Query Frame = 0


Query: 1   MAQAGSLSADTETLCHIFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMM 60
           MAQ  SLSA+T++L HIFSLIEAFRAFDADNDGLIS+AEV GIMGSLGYN+SEQDVN+MM
Sbjct: 1   MAQEASLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDVDKDGLLSIGEFLEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVN 120
           EEGDVD+DGLLS+GEFLEM+A+++E+GE +G YLK+AFEAL ADGDDLVSGEELY+VFVN
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGE-VGRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 LGLEVPLEDCMAIVASMDGDGDGAMFLHDFKLIVNSL 158
           LGL+V LEDCMAIVASMDGDGDGA+FL D KLIVNSL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Cla97C11G221577 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 50/138 (36.23%), Postives = 82/138 (59.42%), Query Frame = 0


Query: 17  IFSLIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEF 76
           I  L   F+ FD + DG ISS E+  IM SLG+ + E+++   + E D   DG ++  EF
Sbjct: 35  IRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEF 94

Query: 77  LEMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVAS 136
           +E+N K M+  ++L + LK AF     DG+  +S EEL++V  +LG E  + +C  ++  
Sbjct: 95  VELNTKGMDQNDVLEN-LKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 154

Query: 137 MDGDGDGAMFLHDFKLIV 155
           +D DGDG +   +FK+++
Sbjct: 155 VDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Cla97C11G221577 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-16
Identity = 42/137 (30.66%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 20  LIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEFLEM 79
           L + F  FD++ DG IS  E+ G+  ++G + +E ++N ++EE D D+DG +++ EF  +
Sbjct: 24  LKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTL 83

Query: 80  NAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVASMDG 139
              +    E     ++ AF+    D + L+S  EL+ V   LG+   +EDC  ++  +D 
Sbjct: 84  CRSSSSAAE-----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 143

Query: 140 DGDGAMFLHDFKLIVNS 157
           DGDG +   +F+ ++ S
Sbjct: 144 DGDGNVNFEEFQKMMTS 155

[Close]

BLAST of Cla97C11G221577 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-16
Identity = 44/136 (32.35%), Postives = 77/136 (56.62%), Query Frame = 0


Query: 22  EAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEFL---- 81
           + F+ FD +NDG IS  E++ ++G+L  N S+++   MM+E D+D +G + + EF+    
Sbjct: 18  KVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQ 77

Query: 82  --EMNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVA 141
             + ++ N  I +     LK AF+    D +  +S  EL+ V  NLG +  ++DC  ++ 
Sbjct: 78  ISDQSSNNSAIRD-----LKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMIN 137

Query: 142 SMDGDGDGAMFLHDFK 152
            +D DGDG +   +FK
Sbjct: 138 KVDSDGDGCVDFEEFK 148

[Close]

BLAST of Cla97C11G221577 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-16
Identity = 42/137 (30.66%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 20  LIEAFRAFDADNDGLISSAEVEGIMGSLGYNMSEQDVNMMMEEGDVDKDGLLSIGEFLEM 79
           L + F  FD++ DG IS  E+ G+  ++G + +E ++N ++EE D D+DG +++ EF  +
Sbjct: 24  LKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTL 83

Query: 80  NAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLEVPLEDCMAIVASMDG 139
              +    E     ++ AF+    D + L+S  EL+ V   LG+   +EDC  ++  +D 
Sbjct: 84  CRSSSSAAE-----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 143

Query: 140 DGDGAMFLHDFKLIVNS 157
           DGDG +   +F+ ++ S
Sbjct: 144 DGDGNVNFEEFQKMMTS 155

[Close]

BLAST of Cla97C11G221577 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 79.7 bits (195), Expect = 2.3e-15
Identity = 46/138 (33.33%), Postives = 82/138 (59.42%), Query Frame = 0


Query: 20  LIEAFRAFDADNDGLISSAEVEGIMGSLGYN-MSEQDVNMMMEEGDVDKDGLLSIGEFLE 79
           +++AF+  D DNDG +S  ++E ++  LG + ++E+++N+M++E D D DG + + E L 
Sbjct: 71  ILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDGDGTIRLEE-LA 130

Query: 80  MNAKNMEIGEMLGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLE-VPLEDCMAIVASM 139
               +++      + LK  FE   AD D L+S +EL  VF  +G E   L+DC  ++A +
Sbjct: 131 SRVVSLDPARD-STELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRMIADV 190

Query: 140 DGDGDGAMFLHDFKLIVN 156
           D DGDG +   +F  +++
Sbjct: 191 DEDGDGFVCFTEFSRMMD 206

The following BLAST results are available for this feature:

BLAST of Cla97C11G221577 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897482.1	1.0e-73	93.04	probable calcium-binding protein CML29 [Benincasa hispida]	[more]
XP_008444124.2	5.8e-69	87.97	PREDICTED: probable calcium-binding protein CML29 [Cucumis melo] >XP_016899854.1...	[more]
XP_004142616.1	9.8e-69	87.97	probable calcium-binding protein CML29 [Cucumis sativus] >KGN54599.1 hypothetica...	[more]
XP_022142920.1	2.7e-66	81.65	probable calcium-binding protein CML29 [Momordica charantia]	[more]
XP_022981068.1	1.9e-64	82.17	probable calcium-binding protein CML29 [Cucurbita maxima]	[more]

BLAST of Cla97C11G221577 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z676	2.2e-26	43.67	Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FYK2	1.7e-18	36.23	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q9M7R0	1.2e-16	34.81	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
O64943	2.6e-16	33.57	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
A0T2M3	4.5e-16	32.86	Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1	[more]

BLAST of Cla97C11G221577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B9P7	2.8e-69	87.97	probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 P...	[more]
A0A5D3D6S0	2.8e-69	87.97	Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0L0M7	4.8e-69	87.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G376140 PE=4 SV=1	[more]
A0A6J1CMA3	1.3e-66	81.65	probable calcium-binding protein CML29 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1J135	9.3e-65	82.17	probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC1114803...	[more]

BLAST of Cla97C11G221577 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	1.2e-19	36.23	EF hand calcium-binding protein family	[more]
AT1G18210.1	2.1e-16	30.66	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	2.1e-16	32.35	calmodulin like 23	[more]
AT1G18210.2	2.1e-16	30.66	Calcium-binding EF-hand family protein	[more]
AT3G10190.1	2.3e-15	33.33	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 93..121 e-value: 70.0 score: 2.9 coord: 19..47 e-value: 0.0062 score: 25.8 coord: 55..83 e-value: 2.8 score: 14.1 coord: 129..157 e-value: 80.0 score: 2.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 20..79 e-value: 5.6E-9 score: 36.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 15..50 score: 12.002819
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 125..158 score: 8.432208
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 51..86 score: 9.018011
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 20..79 e-value: 2.50627E-15 score: 64.4913
None	No IPR available	GENE3D	1.10.238.10		coord: 3..90 e-value: 6.9E-18 score: 67.0
None	No IPR available	GENE3D	1.10.238.10		coord: 92..156 e-value: 6.0E-8 score: 34.7
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 21..154
None	No IPR available	PANTHER	PTHR10891:SF635	CALCIUM-BINDING PROTEIN CML15-RELATED	coord: 21..154
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 28..40
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 17..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C11G221577.1	Cla97C11G221577.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding