Cla97C10G199520 (gene) Watermelon (97103) v2.5

BLAST of Cla97C10G199520 vs. NCBI nr
Match: XP_038905927.1 (cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida])

HSP 1 Score: 848.2 bits (2190), Expect = 3.7e-242
Identity = 444/516 (86.05%), Postives = 456/516 (88.37%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDE+TIEQ  PPSSRSDTAAAVE   VDTNDDSS+T+LCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQVSPPSSRSDTAAAVESCQVDTNDDSSHTHLCFKCKS 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KER+SSSGVGDDGR CSDCFRSNLF KFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERISSSGVGDDGRLCSDCFRSNLFGKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAHKNFEAS+DRSLQVFGVGVAYINETAA SIP+HEID+TI+TIQSVVSSLCPP
Sbjct: 121 VHEMQVKAHKNFEASKDRSLQVFGVGVAYINETAAYSIPSHEIDQTIQTIQSVVSSLCPP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKDLHIVQIE+VYSSN SDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVQIENVYSSNLSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           LLVGTCTSRIACHVISSTVK                                       G
Sbjct: 241 LLVGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPADIQYIDARWE+PVVLPLLDCLAQELN+LCCVD    LKTVELTK+SSSGINDL
Sbjct: 301 QGYSLPADIQYIDARWEIPVVLPLLDCLAQELNLLCCVD---GLKTVELTKQSSSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDL ES NVPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLTESSNVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           SISSESFC VCNGPLNTSDVQTLR FEAGQ SSSILGCTCCSSCQFQILPKDSLSQELFL
Sbjct: 421 SISSESFCAVCNGPLNTSDVQTLRRFEAGQTSSSILGCTCCSSCQFQILPKDSLSQELFL 474

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEA 517
           SLLPP MVARA HGS GNSNSLREQIQEFLLSDDEA
Sbjct: 481 SLLPPSMVARAMHGSFGNSNSLREQIQEFLLSDDEA 474

BLAST of Cla97C10G199520 vs. NCBI nr
Match: XP_004136614.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_031737791.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >KGN59393.1 hypothetical protein Csa_001562 [Cucumis sativus])

HSP 1 Score: 807.4 bits (2084), Expect = 7.3e-230
Identity = 428/518 (82.63%), Postives = 445/518 (85.91%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDE+TIEQD PPSSR DT AA+E R V T DD+SNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYINETAACSIP+ EID+T++TIQSVVSSLC P
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIVQIESVYSSN +DGREKLKKLLD VNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           LLVGTCTSRIACHVISSTVK                                       G
Sbjct: 241 LLVGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLP D+QYIDARWEVPVVLPLLDCLAQELNMLCCVD    LKTVELTKKSSSGINDL
Sbjct: 301 QGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD---GLKTVELTKKSSSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNES+NVPLATKRRQ+RNNFKHVN
Sbjct: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSESFC VCNGPLNTSDV T  +       SSILG  CCSSCQFQILPKDS SQELFL
Sbjct: 421 SLSSESFCAVCNGPLNTSDVLTRHA------GSSILG-ACCSSCQFQILPKDSSSQELFL 469

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARATHGSLGNSNSLR++IQEFLLSDDEAES
Sbjct: 481 SLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

BLAST of Cla97C10G199520 vs. NCBI nr
Match: XP_008443191.1 (PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >KAA0053926.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa] >TYK25478.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-226
Identity = 427/518 (82.43%), Postives = 443/518 (85.52%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDE+TIEQD PPSSR  TAA  E R   TNDD+SNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYINETAA SIP+ EID+TI+TIQSVVSSL  P
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKDLHIV IE+VYSSN SDGREKLKKLLD V+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           LLVGTCTSRIACHVISSTVK                                       G
Sbjct: 241 LLVGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPAD+QYIDARWEVPVVLPLLDCLAQELNMLCC+D    LKTVELTKKSSSGINDL
Sbjct: 301 QGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID---GLKTVELTKKSSSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNES+NVPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSESFC VCNGPLNTSDVQT  +      SSSILG  CCSSCQFQILPKDSLS ELFL
Sbjct: 421 SLSSESFCAVCNGPLNTSDVQTRHA------SSSILG-ACCSSCQFQILPKDSLSHELFL 467

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARATHGSLGNSNSLR +IQEFLLSDD+AES
Sbjct: 481 SLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

BLAST of Cla97C10G199520 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 766.9 bits (1979), Expect = 1.1e-217
Identity = 411/518 (79.34%), Postives = 431/518 (83.20%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQD   ++++D  AA       TN DS +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSSS  GDDGR CSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAHKNFEASRDRSLQVFGVGVAYI+ET+AC IP HEIDRTI+TIQSVVSSL PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            KDLHIVQIESVYSSN SDGREKLKKLLDTV+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           L++GTCTSRIACHVISSTVK                                       G
Sbjct: 241 LVLGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPADIQY+DARWEVPV LPL DCLAQELNMLCC D    LKT ELTK S SGINDL
Sbjct: 301 QGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD---GLKTEELTKTSFSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNES+ VPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSE+FC VCNGPLN SDVQTLRSFEAGQ  SSIL  TCCSSCQFQILPKDS+SQEL L
Sbjct: 421 SLSSETFCAVCNGPLNMSDVQTLRSFEAGQ-VSSILAGTCCSSCQFQILPKDSISQELLL 468

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARA HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 481 SLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of Cla97C10G199520 vs. NCBI nr
Match: XP_023539180.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 765.8 bits (1976), Expect = 2.4e-217
Identity = 410/518 (79.15%), Postives = 430/518 (83.01%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSS CQSNCYRNQ DE T+EQD   ++++D  AA       TN DS +T+LCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSSS  GDDGR CSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAHKNFEASRDRSLQVFGVGVAYI+ET+AC IP HEIDRTI+ IQSVVSSL PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQSVVSSLSPP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            KDLHIVQIESVYSSN SDGREKLKKLLDTV+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           L++GTCTSRIACHVISSTVK                                       G
Sbjct: 241 LVLGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPADIQY+DARWEVPV LPL DCLAQELNMLCC D    LKTVELTK S SGINDL
Sbjct: 301 QGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD---GLKTVELTKTSFSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNES+ VPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSE+FC VCNGPLN SDVQTLRSFEAGQ  SSIL  TCCSSCQFQILPKDS+SQEL L
Sbjct: 421 SLSSETFCAVCNGPLNMSDVQTLRSFEAGQ-VSSILAGTCCSSCQFQILPKDSISQELLL 468

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARA HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 481 SLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of Cla97C10G199520 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 482.3 bits (1240), Expect = 7.1e-135
Identity = 269/516 (52.13%), Postives = 346/516 (67.05%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSS C+S CY  + D          S   D A          +   ++ ++C KCK 
Sbjct: 1   MACNSSGCESGCYDREKDNG--------SKIVDDA---------VSGGGNHESVCVKCKC 60

Query: 61  KERMS-SSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQ 120
              M+   G  DDGR C+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG  SRV+LQ
Sbjct: 61  NAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQ 120

Query: 121 FVHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCP 180
           FVHE+Q+KA KN+EASRDRSL VFGVGVA+++ETAA    + E+   IE ++  VS L P
Sbjct: 121 FVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSP 180

Query: 181 PRKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYN 240
           P KDLH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ +L+MLSLQK+AAENGYN
Sbjct: 181 PAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYN 240

Query: 241 RLLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAG 300
           RL++G+CTSRIA HV+++TVK                                       
Sbjct: 241 RLVLGSCTSRIASHVLTATVK--------------------------------------- 300

Query: 301 GQGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGIND 360
           G+GYSL ADIQ++DARW+VP+VLPL DC+  E+  LC +D    LKTVEL  +S  GIND
Sbjct: 301 GRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLD---GLKTVELACRSQCGIND 360

Query: 361 LVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHV 420
           LVSSFVALLQ ENPSRE TIVRTA KLTPF F++IP+ ++S NVP+AT+RR KR N K+ 
Sbjct: 361 LVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS-NVPMATQRRLKRFNLKYD 420

Query: 421 NSISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELF 480
            S+++E+FC +CNGPLN SD   L +FE GQE S +L   CCSSC+FQILP+D  S E F
Sbjct: 421 GSMTTEAFCPICNGPLNRSDSSELDTFEEGQE-SDVLYAACCSSCRFQILPQDGSSLEQF 455

Query: 481 LSLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDE 516
            S LP +M+++  H  + +   LRE+I++ LL DDE
Sbjct: 481 SSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

BLAST of Cla97C10G199520 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.7e-99
Identity = 213/492 (43.29%), Postives = 294/492 (59.76%), Query Frame = 0

Query: 27  PPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKC-KSKERMSSSGVGDDGRCCSDCFRSNLF 86
           P  S S +AAAVE       +     +L  +C K     ++  V      C +CFR+NL 
Sbjct: 16  PHCSSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLL 75

Query: 87  AKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHKNFEASRDRSLQVFGV 146
            KF+ AV SNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA ++++ S  ++L VFGV
Sbjct: 76  GKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVSNSQALPVFGV 135

Query: 147 GVAYINETAACSIPAHEIDRTIETIQSVVSSLCPPRKDLHIVQIESVYSSNFSDGREKLK 206
           GVA+++E+  CS P  EI+  IE I+S+VSSL    K +HI ++E V+S+   DG  +L+
Sbjct: 136 GVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIARLEDVFSTESEDGERRLR 195

Query: 207 KLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKAKAML 266
           + +D + D TG+ED +  LRMLSLQKIA ENGY ++++G+C S IACHV+S+TVK     
Sbjct: 196 EAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCASAIACHVLSATVK----- 255

Query: 267 ALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGGQGYSLPADIQYIDARWEVPVVLPLL 326
                                             GQGYSLPAD+QY+D RWE+PVVLPL 
Sbjct: 256 ----------------------------------GQGYSLPADVQYVDTRWEIPVVLPLR 315

Query: 327 DCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGK 386
           DCLAQEL +LC +D   SLKT +   + S+GIN LV+SF+  L+ ENPSRE TIVRTA K
Sbjct: 316 DCLAQELTLLCELD---SLKTQQHLDRPSNGINSLVASFIKRLREENPSREHTIVRTAQK 375

Query: 387 LTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVNSISSESFCTVCNGPLNTSDVQTLRS 446
           L PF+F++       D +P  ++ R K   F    S  SE  C +C  P + S++Q L S
Sbjct: 376 LKPFSFNKFSADGYHDFLP--SRLRPKFQKFDSDESTFSEILCLMCGSPFSESELQNLES 435

Query: 447 FE-AGQESSSILGCTCCSSCQFQILPKDSLSQELFLSLLPPYMVARATHGSLGNSNS-LR 506
            +   Q+   +    CC SC FQILP      E F SLLP     +    ++ +S+S LR
Sbjct: 436 TKHKAQKKIDLYTAHCCQSCYFQILPAGENLNEHFFSLLPKLWTGKM--DTISDSHSLLR 461

Query: 507 EQIQEFLLSDDE 516
           +QI+E+LL +++
Sbjct: 496 DQIEEYLLEEND 461

BLAST of Cla97C10G199520 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 8.2e-99
Identity = 213/492 (43.29%), Postives = 294/492 (59.76%), Query Frame = 0

Query: 27  PPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKC-KSKERMSSSGVGDDGRCCSDCFRSNLF 86
           P  S S +AAAVE       +     +L  +C K     ++  V      C +CFR+NL 
Sbjct: 16  PHCSSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLL 75

Query: 87  AKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHKNFEASRDRSLQVFGV 146
            KF+ AV SNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA ++++ S  ++L VFGV
Sbjct: 76  GKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVSNSQALPVFGV 135

Query: 147 GVAYINETAACSIPAHEIDRTIETIQSVVSSLCPPRKDLHIVQIESVYSSNFSDGREKLK 206
           GVA+++E+  CS P  EI+  IE I+S+VSSL    K +HIV++E V+S+   DG  +L+
Sbjct: 136 GVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIVRLEDVFSTESEDGERRLR 195

Query: 207 KLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKAKAML 266
           + +D + D TG+ED +  LRMLSLQKIA ENGY ++++G+C S IACHV+S+TVK     
Sbjct: 196 EAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCASAIACHVLSATVK----- 255

Query: 267 ALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGGQGYSLPADIQYIDARWEVPVVLPLL 326
                                             GQGYSLPAD+QY+D RWE+PVVLPL 
Sbjct: 256 ----------------------------------GQGYSLPADVQYVDTRWEIPVVLPLR 315

Query: 327 DCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGK 386
           DCLAQEL +LC +D   SLKT +   + S+GIN LV+SF+  L+ ENPSRE TIVRTA K
Sbjct: 316 DCLAQELTLLCELD---SLKTQQHLDRPSNGINSLVASFIKRLREENPSREHTIVRTAQK 375

Query: 387 LTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVNSISSESFCTVCNGPLNTSDVQTLRS 446
           L PF+F++       D +P  ++ R K        S  SE  C +C  P + S++Q L S
Sbjct: 376 LKPFSFNKFSADGYHDFLP--SRLRPKFQKVDSDESTFSEILCLMCGSPFSESELQNLES 435

Query: 447 FE-AGQESSSILGCTCCSSCQFQILPKDSLSQELFLSLLPPYMVARATHGSLGNSNS-LR 506
            +   Q+   +    CC SC FQILP      E F SLLP     +    ++ +S+S LR
Sbjct: 436 TKHKAQKKIDLYTAHCCQSCYFQILPAGENLNEHFFSLLPKLWTGKM--DTISDSHSLLR 461

Query: 507 EQIQEFLLSDDE 516
           +QI+E+LL +++
Sbjct: 496 DQIEEYLLEEND 461

BLAST of Cla97C10G199520 vs. ExPASy Swiss-Prot
Match: Q28ES8 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Xenopus tropicalis OX=8364 GN=ctu2 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.6e-20
Identity = 139/545 (25.50%), Postives = 213/545 (39.08%), Query Frame = 0

Query: 40  PRPVDTNDDSSNTNLCFKCKSKERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMIS 99
           P   DT    S    C KCK    +     GD    C  CF+     KFR  +  N +I 
Sbjct: 10  PELQDTTQGKSLGKTCMKCKESSAVLLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIY 69

Query: 100 PSDKVLVAFSGGPCSRVALQFVHEMQVKAHKNFEASRDRSLQV-FGVGVAYINETAACSI 159
           P +KVL+AFSGGP S   +Q V E           SRD   ++ F  G+ +I+E   C +
Sbjct: 70  PGEKVLLAFSGGPSSSAMVQQVQE---------GMSRDAPKKLRFVPGILFIDEGTTCGM 129

Query: 160 PAHEIDRTIETIQSVVSSLCPPRKDLHIVQIESVYS-------SNFSDGREKLKKLLD-- 219
              E  + +  +Q+V+     P    +IV +E V+S           + R   K+ +D  
Sbjct: 130 SHEERQQNMSEVQNVLQQTGFP---FYIVSLEQVFSLPGSILQRGVPEQRANYKQEVDRF 189

Query: 220 ----TVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACH---VISSTVKAK 279
                    TG  D++  L  L +    +    +++   TC      H   +I     AK
Sbjct: 190 MVQQQAQGETGCSDILENLAKLGVNVSESSRESDKMFQSTCRHPPDTHTQKLIQLFASAK 249

Query: 280 AMLA---LLSAGKTGM---LEGEKGGHHYLSGDTPTK-----IEKIAGGQGYSLPADIQY 339
            + A   LL   ++ +   +    G    +SG++ T+     +  I+ G+G  LP D  +
Sbjct: 250 TLTAKHQLLHTLRSHLILHIARTCGYSKVMSGESCTRLSVSLLSNISLGRGAFLPLDTGF 309

Query: 340 IDARW-EVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKK--SSSGINDLVSSFVALL 399
            D+R+ +V ++ P+ +   +E+     +    S+    L  K   +S I  L   F+  L
Sbjct: 310 CDSRYGDVDIIRPMREYSLKEIAFYNRLFHVSSVFIPALNTKVLENSSIQQLSEVFINRL 369

Query: 400 QVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVNSISSESFC 459
           Q + PS  ST+ RT+ KL   N S+I D N+        K R                 C
Sbjct: 370 QADFPSTVSTVYRTSEKL---NVSKI-DANQG----TCAKER-----------------C 429

Query: 460 TVCNGPLNTSDVQTLRSFEAGQESSSI--------------LGCTCCSS----------- 516
            +C  PL+T  V    +F A Q S  I               G +CC             
Sbjct: 430 LLCLSPLDT-QVGEASAFHATQISHYISQKILMKFNDPANSSGKSCCQEGKCCKGPGYGD 489

BLAST of Cla97C10G199520 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.4e-18
Identity = 129/527 (24.48%), Postives = 211/527 (40.04%), Query Frame = 0

Query: 40  PRPVDTNDDSSNTNLCFKCKSKERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMIS 99
           P   D     S   +C KCK          GD    C  CF+     KFR  +  N +I 
Sbjct: 10  PEVKDAKQGKSLGKICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIY 69

Query: 100 PSDKVLVAFSGGPCSRVALQFVHEMQVKAHKNFEASRDRSLQV-FGVGVAYINETAACSI 159
           P +KVL+A+SGGP S   ++ V E           SRD   ++ F  G+ +I+E  AC +
Sbjct: 70  PGEKVLLAYSGGPSSSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGM 129

Query: 160 PAHEIDRTIETIQSVVSSLCPPRKDLHIVQIESVYSSNFS-------DGREKLKKLLD-- 219
              E  + +  I SV+     P    HIV +E V+S   S       + R   K+ +D  
Sbjct: 130 SWEERQQILSEICSVLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRF 189

Query: 220 -TVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACH--------VISSTVK 279
                  G       L  L +    +    +++   TC+     H          + T+ 
Sbjct: 190 LVQEREQGDAGCSEMLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLT 249

Query: 280 AK-AMLALLSAGKTGMLEGEKGGHHYLSGDTPTK-----IEKIAGGQGYSLPADIQYIDA 339
           AK  +L  L +     +    G    ++G++ T+     +  ++ G+G  LP D  + D+
Sbjct: 250 AKQQLLHTLRSHLILHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDS 309

Query: 340 RW-EVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSS--SGINDLVSSFVALLQVE 399
           R+ +V ++ P+ +  ++E+           +    L  K+S  S I  L   FV  LQ +
Sbjct: 310 RYGDVDIIRPMREYSSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQAD 369

Query: 400 NPSRESTIVRTAGKLTPFNFSRIPDLNESDNV-----PLATKR-RQKRNNFKHVNSISSE 459
            PS  ST+ RT+ KL         +    D       PL T+  +    +   ++   S+
Sbjct: 370 FPSTVSTLYRTSEKLNVSKIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQ 429

Query: 460 SFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTC--------------CSSCQFQILPK 519
                 N   N SD    +  +  +E+    G TC              C SC+  +  K
Sbjct: 430 KIPMKSNDLANNSDKSCCQGGQGCKEAG--YGDTCQSRALQTPSFVHMLCYSCRLTV--K 489

BLAST of Cla97C10G199520 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.5e-230
Identity = 428/518 (82.63%), Postives = 445/518 (85.91%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDE+TIEQD PPSSR DT AA+E R V T DD+SNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYINETAACSIP+ EID+T++TIQSVVSSLC P
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIVQIESVYSSN +DGREKLKKLLD VNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           LLVGTCTSRIACHVISSTVK                                       G
Sbjct: 241 LLVGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLP D+QYIDARWEVPVVLPLLDCLAQELNMLCCVD    LKTVELTKKSSSGINDL
Sbjct: 301 QGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD---GLKTVELTKKSSSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNES+NVPLATKRRQ+RNNFKHVN
Sbjct: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSESFC VCNGPLNTSDV T  +       SSILG  CCSSCQFQILPKDS SQELFL
Sbjct: 421 SLSSESFCAVCNGPLNTSDVLTRHA------GSSILG-ACCSSCQFQILPKDSSSQELFL 469

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARATHGSLGNSNSLR++IQEFLLSDDEAES
Sbjct: 481 SLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

BLAST of Cla97C10G199520 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 8.2e-227
Identity = 427/518 (82.43%), Postives = 443/518 (85.52%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDE+TIEQD PPSSR  TAA  E R   TNDD+SNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYINETAA SIP+ EID+TI+TIQSVVSSL  P
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKDLHIV IE+VYSSN SDGREKLKKLLD V+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           LLVGTCTSRIACHVISSTVK                                       G
Sbjct: 241 LLVGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPAD+QYIDARWEVPVVLPLLDCLAQELNMLCC+D    LKTVELTKKSSSGINDL
Sbjct: 301 QGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID---GLKTVELTKKSSSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNES+NVPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSESFC VCNGPLNTSDVQT  +      SSSILG  CCSSCQFQILPKDSLS ELFL
Sbjct: 421 SLSSESFCAVCNGPLNTSDVQTRHA------SSSILG-ACCSSCQFQILPKDSLSHELFL 467

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARATHGSLGNSNSLR +IQEFLLSDD+AES
Sbjct: 481 SLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

BLAST of Cla97C10G199520 vs. ExPASy TrEMBL
Match: A0A5D3DPK4 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006240 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 8.2e-227
Identity = 427/518 (82.43%), Postives = 443/518 (85.52%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDE+TIEQD PPSSR  TAA  E R   TNDD+SNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+SGVGDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYINETAA SIP+ EID+TI+TIQSVVSSL  P
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKDLHIV IE+VYSSN SDGREKLKKLLD V+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           LLVGTCTSRIACHVISSTVK                                       G
Sbjct: 241 LLVGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPAD+QYIDARWEVPVVLPLLDCLAQELNMLCC+D    LKTVELTKKSSSGINDL
Sbjct: 301 QGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID---GLKTVELTKKSSSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNES+NVPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSESFC VCNGPLNTSDVQT  +      SSSILG  CCSSCQFQILPKDSLS ELFL
Sbjct: 421 SLSSESFCAVCNGPLNTSDVQTRHA------SSSILG-ACCSSCQFQILPKDSLSHELFL 467

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARATHGSLGNSNSLR +IQEFLLSDD+AES
Sbjct: 481 SLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

BLAST of Cla97C10G199520 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 5.3e-218
Identity = 411/518 (79.34%), Postives = 431/518 (83.20%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQD   ++++D  AA       TN DS +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSSS  GDDGR CSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAHKNFEASRDRSLQVFGVGVAYI+ET+AC IP HEIDRTI+TIQSVVSSL PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            KDLHIVQIESVYSSN SDGREKLKKLLDTV+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           L++GTCTSRIACHVISSTVK                                       G
Sbjct: 241 LVLGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPADIQY+DARWEVPV LPL DCLAQELNMLCC D    LKT ELTK S SGINDL
Sbjct: 301 QGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD---GLKTEELTKTSFSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNES+ VPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSE+FC VCNGPLN SDVQTLRSFEAGQ  SSIL  TCCSSCQFQILPKDS+SQEL L
Sbjct: 421 SLSSETFCAVCNGPLNMSDVQTLRSFEAGQ-VSSILAGTCCSSCQFQILPKDSISQELLL 468

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARA HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 481 SLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of Cla97C10G199520 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 1.0e-216
Identity = 409/518 (78.96%), Postives = 430/518 (83.01%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+EQD   ++++D  AA       T  DS +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  KERMSSSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSSS  GDDGR CSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCPP 180
           VHEMQVKAHKNFEASRDRSL VFGVGVAYI+ET+AC IP HEIDRTI+TIQSVVSSL PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            KDLHIVQIESVYSSN SDGREKLKKLLDTV+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAGG 300
           L++GTCTSRIACHVISSTVK                                       G
Sbjct: 241 LVLGTCTSRIACHVISSTVK---------------------------------------G 300

Query: 301 QGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGINDL 360
           QGYSLPADIQY+DARWEVPV LPL DCLAQELNMLCC D    LKTVELTK S SGINDL
Sbjct: 301 QGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD---GLKTVELTKTSFSGINDL 360

Query: 361 VSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHVN 420
           VSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNES+ VPLATKRRQKRNNFKHVN
Sbjct: 361 VSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVN 420

Query: 421 SISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELFL 480
           S+SSE+FC VCNGPLN SDVQTLRSFEAGQ  SSIL  TCCSSCQFQILPKDS+SQEL L
Sbjct: 421 SLSSETFCAVCNGPLNMSDVQTLRSFEAGQ-VSSILAGTCCSSCQFQILPKDSISQELLL 468

Query: 481 SLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDEAES 519
           SLLPP MVARA HGS  NSNSLRE+IQEFLLSDDE ES
Sbjct: 481 SLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

BLAST of Cla97C10G199520 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 482.3 bits (1240), Expect = 5.0e-136
Identity = 269/516 (52.13%), Postives = 346/516 (67.05%), Query Frame = 0

Query: 1   MACNSSACQSNCYRNQHDENTIEQDLPPSSRSDTAAAVEPRPVDTNDDSSNTNLCFKCKS 60
           MACNSS C+S CY  + D          S   D A          +   ++ ++C KCK 
Sbjct: 1   MACNSSGCESGCYDREKDNG--------SKIVDDA---------VSGGGNHESVCVKCKC 60

Query: 61  KERMS-SSGVGDDGRCCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQ 120
              M+   G  DDGR C+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG  SRV+LQ
Sbjct: 61  NAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQ 120

Query: 121 FVHEMQVKAHKNFEASRDRSLQVFGVGVAYINETAACSIPAHEIDRTIETIQSVVSSLCP 180
           FVHE+Q+KA KN+EASRDRSL VFGVGVA+++ETAA    + E+   IE ++  VS L P
Sbjct: 121 FVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSP 180

Query: 181 PRKDLHIVQIESVYSSNFSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYN 240
           P KDLH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ +L+MLSLQK+AAENGYN
Sbjct: 181 PAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYN 240

Query: 241 RLLVGTCTSRIACHVISSTVKAKAMLALLSAGKTGMLEGEKGGHHYLSGDTPTKIEKIAG 300
           RL++G+CTSRIA HV+++TVK                                       
Sbjct: 241 RLVLGSCTSRIASHVLTATVK--------------------------------------- 300

Query: 301 GQGYSLPADIQYIDARWEVPVVLPLLDCLAQELNMLCCVDRSGSLKTVELTKKSSSGIND 360
           G+GYSL ADIQ++DARW+VP+VLPL DC+  E+  LC +D    LKTVEL  +S  GIND
Sbjct: 301 GRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLD---GLKTVELACRSQCGIND 360

Query: 361 LVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESDNVPLATKRRQKRNNFKHV 420
           LVSSFVALLQ ENPSRE TIVRTA KLTPF F++IP+ ++S NVP+AT+RR KR N K+ 
Sbjct: 361 LVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS-NVPMATQRRLKRFNLKYD 420

Query: 421 NSISSESFCTVCNGPLNTSDVQTLRSFEAGQESSSILGCTCCSSCQFQILPKDSLSQELF 480
            S+++E+FC +CNGPLN SD   L +FE GQE S +L   CCSSC+FQILP+D  S E F
Sbjct: 421 GSMTTEAFCPICNGPLNRSDSSELDTFEEGQE-SDVLYAACCSSCRFQILPQDGSSLEQF 455

Query: 481 LSLLPPYMVARATHGSLGNSNSLREQIQEFLLSDDE 516
            S LP +M+++  H  + +   LRE+I++ LL DDE
Sbjct: 481 SSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455