Cla97C03G054280 (gene) Watermelon (97103) v2.5

Overview
NameCla97C03G054280
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionCytochrome P450 71A1-like
LocationCla97Chr03: 3500167 .. 3501425 (+)
RNA-Seq ExpressionCla97C03G054280
SyntenyCla97C03G054280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTTTCCCCTTACGGTTCTCACTGGCGCCAAATCAGAAAAATATGCATTATCGAACTCCTTAGTGCCAAAAGGGTCCAATCCTTTGCCCATATTCGACAACAAGAAGCTGCTCGTTTGGTTCACAGAATCTCCCAGTCTAACAATCCGGTGGATTTGTCGAGCCTCGTGGCGTTGTATGCCAATGAAGTGCTCTGTCGAATGGCGCTCGGGAGGGAATTTTCCGCCGGTGGTGAGTACCATCTTCATGGGATTCAAGCACTGCTTGAAGAGTATCAGATTCTGGTTGGTGGGTTCTTTTTTAGCGGATTTGTTCCCTTCTTTGTCATTTCTCAGTACGCTAACAGGGACGAAATCGAGGCTTGTGAGGACGTTTAAAGCGTTCGATAAGTTGTTTGATCAAGTAATAGCTGAACATCTTAGTCCTCAGCGAGAGAAACTTGGAGAATCTAAGGATCTTGTTGATGTTTTGCTTGATATACAGAGGAATGGATCTGATGATAAATTTCCTCTTACTATGGATAATGTTAAAGGCATTATTTTGGTATAACTCCATAAATTCCTCCGTTTTCCTCTGTTTTTATTGAAACTGACTAACCTATGTGGGAAATTTTTGTAGGACATGTTTGTTGCAGGGACAGACACGACTTTTATAGTTCTTGATTGGGGGATGACTGAGCTCCTAACACACCCAAAAGCCATGAAAAGAGCACAAGACGAAATTCGAAGCATTGTTGGTGAGAGAAGAATTGTATCAGAGAGTGATCTTTCAGAAATGCTTTATTTGAAAGCTGTGGTTAAAGAGGTGCTCCGATTGCATCCACCAGCGCCGGTTTCAGTTCCGAGAGAAACTATGGAAGATGTGAGCATTGAAGGCTTTGACATTCCAGCAAAAACGAGAGTTTTTGTGAATCTTTGGGCAATTGGGAGAGACCCAGAATCGTGGAAAGACCCAGAAAGTTTCGAGCCAGAGATTTATGGAGATTGAAGTTGATTACAGAGGGTTGGATTTTGAGTTCATTCCGCTCGGGGCTGGAAGAAGGATCTGCCCAGGTATCTCTATGGGGATTGTCACGATTGAGCTTGCTTTAGCTCAGATTTTGCATAGCTTTGATTGGGAACTTCCCAGTGGGATTGAAGCTAAAGATTTGGATATGACTGAAGTTTTTGGTATCACAATGCATAGAAAAGCTCACTTGGAGGTTGTGGTACTTCAGCTCATCCATCTCAAATTAATTAGTTTTCCTTTGGGTTAA

mRNA sequence

ATGGTTTTTTCCCCTTACGGTTCTCACTGGCGCCAAATCAGAAAAATATGCATTATCGAACTCCTTAGTGCCAAAAGGGTCCAATCCTTTGCCCATATTCGACAACAAGAAGCTGCTCGTTTGGTTCACAGAATCTCCCAGTCTAACAATCCGGTGGATTTGTCGAGCCTCGTGGCGTTGTATGCCAATGAAGTGCTCTGTCGAATGGCGCTCGGGAGGGAATTTTCCGCCGGTGGTGAGTACCATCTTCATGGGATTCAAGCACTGCTTGAAGAGTATCAGATTCTGGTTGGGACGAAATCGAGGCTTGTGAGGACGTTTAAAGCGTTCGATAAGTTGTTTGATCAAGTAATAGCTGAACATCTTAGTCCTCAGCGAGAGAAACTTGGAGAATCTAAGGATCTTGTTGATGTTTTGCTTGATATACAGAGGAATGGATCTGATGATAAATTTCCTCTTACTATGGATAATGTTAAAGGCATTATTTTGGACATGTTTGTTGCAGGGACAGACACGACTTTTATAGTTCTTGATTGGGGGATGACTGAGCTCCTAACACACCCAAAAGCCATGAAAAGAGCACAAGACGAAATTCGAAGCATTGTTGGTGAGAGAAGAATTGTATCAGAGAGTGATCTTTCAGAAATGCTTTATTTGAAAGCTGTGGTTAAAGAGGTGCTCCGATTGCATCCACCAGCGCCGGTTTCAGTTCCGAGAGAAACTATGGAAGATAATCGTGGAAAGACCCAGAAAGTTTCGAGCCAGAGATTTATGGAGATTGAAGTTGATTACAGAGGGTTGGATTTTGAGTTCATTCCGCTCGGGGCTGGAAGAAGGATCTGCCCAGGTATCTCTATGGGGATTGTCACGATTGAGCTTGCTTTAGCTCAGATTTTGCATAGCTTTGATTGGGAACTTCCCAGTGGGATTGAAGCTAAAGATTTGGATATGACTGAAGTTTTTGGTATCACAATGCATAGAAAAGCTCACTTGGAGGTTGTGGTACTTCAGCTCATCCATCTCAAATTAATTAGTTTTCCTTTGGGTTAA

Coding sequence (CDS)

ATGGTTTTTTCCCCTTACGGTTCTCACTGGCGCCAAATCAGAAAAATATGCATTATCGAACTCCTTAGTGCCAAAAGGGTCCAATCCTTTGCCCATATTCGACAACAAGAAGCTGCTCGTTTGGTTCACAGAATCTCCCAGTCTAACAATCCGGTGGATTTGTCGAGCCTCGTGGCGTTGTATGCCAATGAAGTGCTCTGTCGAATGGCGCTCGGGAGGGAATTTTCCGCCGGTGGTGAGTACCATCTTCATGGGATTCAAGCACTGCTTGAAGAGTATCAGATTCTGGTTGGGACGAAATCGAGGCTTGTGAGGACGTTTAAAGCGTTCGATAAGTTGTTTGATCAAGTAATAGCTGAACATCTTAGTCCTCAGCGAGAGAAACTTGGAGAATCTAAGGATCTTGTTGATGTTTTGCTTGATATACAGAGGAATGGATCTGATGATAAATTTCCTCTTACTATGGATAATGTTAAAGGCATTATTTTGGACATGTTTGTTGCAGGGACAGACACGACTTTTATAGTTCTTGATTGGGGGATGACTGAGCTCCTAACACACCCAAAAGCCATGAAAAGAGCACAAGACGAAATTCGAAGCATTGTTGGTGAGAGAAGAATTGTATCAGAGAGTGATCTTTCAGAAATGCTTTATTTGAAAGCTGTGGTTAAAGAGGTGCTCCGATTGCATCCACCAGCGCCGGTTTCAGTTCCGAGAGAAACTATGGAAGATAATCGTGGAAAGACCCAGAAAGTTTCGAGCCAGAGATTTATGGAGATTGAAGTTGATTACAGAGGGTTGGATTTTGAGTTCATTCCGCTCGGGGCTGGAAGAAGGATCTGCCCAGGTATCTCTATGGGGATTGTCACGATTGAGCTTGCTTTAGCTCAGATTTTGCATAGCTTTGATTGGGAACTTCCCAGTGGGATTGAAGCTAAAGATTTGGATATGACTGAAGTTTTTGGTATCACAATGCATAGAAAAGCTCACTTGGAGGTTGTGGTACTTCAGCTCATCCATCTCAAATTAATTAGTTTTCCTTTGGGTTAA

Protein sequence

MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVALYANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILVGTKSRLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGIILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKAVVKEVLRLHPPAPVSVPRETMEDNRGKTQKVSSQRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDLDMTEVFGITMHRKAHLEVVVLQLIHLKLISFPLG
Homology
BLAST of Cla97C03G054280 vs. NCBI nr
Match: TYJ99205.1 (cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 523.9 bits (1348), Expect = 1.1e-144
Identity = 272/379 (71.77%), Postives = 302/379 (79.68%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WRQ+RKICI++LL+AKRVQSFA IRQQE +RLV+RIS S NPVDL++L+A+
Sbjct: 122 IAFSPYGSYWRQVRKICILQLLNAKRVQSFAFIRQQEVSRLVNRISHSTNPVDLTNLLAM 181

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCRMALGREFS GGEYHLHGIQ LLEEYQIL+                   GTKS
Sbjct: 182 YANDVLCRMALGREFSTGGEYHLHGIQELLEEYQILLGGFFFADLFPSLSFISTLTGTKS 241

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLV+TFKAFDKL DQVIAEH S  REKLGESKDLVDVLLDIQ+NG +DKF LTMDNVKGI
Sbjct: 242 RLVKTFKAFDKLVDQVIAEHQSHDREKLGESKDLVDVLLDIQKNGFEDKFLLTMDNVKGI 301

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMFVAGTDTTFI LDWGMTEL+THP  MKRAQ EIR +VG+RR V+ESDL EM YLKA
Sbjct: 302 ILDMFVAGTDTTFIALDWGMTELITHPNVMKRAQSEIRRVVGDRRNVTESDLLEMPYLKA 361

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPP PVSVPRET ED +        KT                   +    +
Sbjct: 362 VVKEVLRLHPPVPVSVPRETTEDVKIEGYDIPAKTRVFVNIWAIGRDPESWKDPETFEPE 421

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDY+GL+FEFIP GAGRRICPGI+MGIVTIELALAQILHSFDWELP G+EAKDL
Sbjct: 422 RFLESEVDYKGLNFEFIPFGAGRRICPGIAMGIVTIELALAQILHSFDWELPKGMEAKDL 481

BLAST of Cla97C03G054280 vs. NCBI nr
Match: XP_031738204.1 (cytochrome P450 71A1-like [Cucumis sativus] >KAE8652612.1 hypothetical protein Csa_013068 [Cucumis sativus])

HSP 1 Score: 519.2 bits (1336), Expect = 2.7e-143
Identity = 269/379 (70.98%), Postives = 305/379 (80.47%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WRQ+RK CI++LLSAKRVQSFA IRQQE ARLV+RIS S N VDL+SL+A+
Sbjct: 124 IAFSPYGSYWRQLRKFCILQLLSAKRVQSFAFIRQQEVARLVNRISHSINHVDLTSLLAM 183

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCR+ALGREFS GGEYHLHGI+ LLEE+Q+L+                   GTKS
Sbjct: 184 YANDVLCRIALGREFSTGGEYHLHGIKELLEEFQMLLGGFFFADLFPSLSFISTLTGTKS 243

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLV+TFK FDKLFDQVIAEH SP REKLGESKDLVDVLLDIQ+NG +DKF LTMDNVKGI
Sbjct: 244 RLVKTFKGFDKLFDQVIAEHQSPNREKLGESKDLVDVLLDIQKNGFEDKFLLTMDNVKGI 303

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMFVAG+DTT+I LDWGMTEL+THP AM+RAQ EIR +VG+RR V+ESD+ EM YLKA
Sbjct: 304 ILDMFVAGSDTTYIALDWGMTELITHPNAMERAQSEIRRVVGDRRNVTESDVLEMPYLKA 363

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPPAPVSVPRET+ED R        KT                   +    +
Sbjct: 364 VVKEVLRLHPPAPVSVPRETLEDVRIEGYDIPAKTRVFVNVWAIGRDPESWKDPENFEPE 423

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDY+GL+FEFIP GAGRRICPGI+MGIVTIELALAQILHS+DWELP+GIEAKDL
Sbjct: 424 RFLESEVDYKGLNFEFIPFGAGRRICPGITMGIVTIELALAQILHSYDWELPTGIEAKDL 483

BLAST of Cla97C03G054280 vs. NCBI nr
Match: XP_031743079.1 (cytochrome P450 71A1-like [Cucumis sativus] >KAE8652614.1 hypothetical protein Csa_013681 [Cucumis sativus])

HSP 1 Score: 518.8 bits (1335), Expect = 3.5e-143
Identity = 268/379 (70.71%), Postives = 302/379 (79.68%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WRQ+RKICI++LL+AKRVQSFA IRQQE ARLV+RIS S + VDL+SL+A+
Sbjct: 124 IAFSPYGSYWRQVRKICILQLLNAKRVQSFAFIRQQEVARLVNRISHSTDHVDLTSLLAI 183

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCRMALGREFS GGEYHLHGIQ LLEEYQ+L+                   GTKS
Sbjct: 184 YANDVLCRMALGREFSTGGEYHLHGIQELLEEYQVLLGGFSFADLFPSLSFISTLTGTKS 243

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLV+TFKAFDKL D+VIAEH SP REKLG+SKDLVDVLLDIQ+NG +DKF LTMDNVKGI
Sbjct: 244 RLVKTFKAFDKLVDKVIAEHQSPDREKLGQSKDLVDVLLDIQKNGFEDKFFLTMDNVKGI 303

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMFVAGTDTTFI LDWGMTEL+THP AMKRAQ EIR +VG+RR V++SD+ EM YLKA
Sbjct: 304 ILDMFVAGTDTTFIALDWGMTELITHPNAMKRAQSEIRRVVGDRRNVTDSDVLEMPYLKA 363

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPP PVS PRET+ED R        KT                   +    +
Sbjct: 364 VVKEVLRLHPPVPVSTPRETIEDVRIEGYDIPAKTRVFVNVWAIGRDPESWKDPETFEPE 423

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDY+GL+FEFIP GAGRRICPGI+MGI TIEL LAQILHSFDWELP+G+EAKDL
Sbjct: 424 RFLESEVDYKGLNFEFIPFGAGRRICPGITMGIATIELGLAQILHSFDWELPNGVEAKDL 483

BLAST of Cla97C03G054280 vs. NCBI nr
Match: XP_038895182.1 (cytochrome P450 71AP13-like [Benincasa hispida])

HSP 1 Score: 515.8 bits (1327), Expect = 3.0e-142
Identity = 270/379 (71.24%), Postives = 297/379 (78.36%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WR +RKICI+ELLSAKRV+SFA IRQQE  RLV RISQS + VDLSS +AL
Sbjct: 126 IAFSPYGSYWRHVRKICILELLSAKRVESFALIRQQEVTRLVDRISQSKSRVDLSSFLAL 185

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCR ALGREFSA GEYHLHGIQ LLEEYQIL+                   G KS
Sbjct: 186 YANDVLCRAALGREFSASGEYHLHGIQELLEEYQILLGGFCIGDLFPSLAFLSKFTGMKS 245

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RL+RT KAFDKLFDQVIAEHL+P+RE + ESKDLVDVLLDIQ+NGS DK  LTMDNVK I
Sbjct: 246 RLMRTAKAFDKLFDQVIAEHLNPERENMDESKDLVDVLLDIQKNGSYDKISLTMDNVKAI 305

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMF AGTDTTFI LDWGMTEL+THP+ MKRAQ+EIRSIVGERRIVSESD+ EM YLKA
Sbjct: 306 ILDMFAAGTDTTFITLDWGMTELITHPETMKRAQNEIRSIVGERRIVSESDVLEMPYLKA 365

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPP PVSVPRET ED R        KT                   +    +
Sbjct: 366 VVKEVLRLHPPVPVSVPRETREDVRIEGYDIPAKTRVFVNLWAIGRDPESWKNPESFEPE 425

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF++ +VDY+GLDFEFIP G GRRICPGI+MGI T+ELALAQILHSFDWELPSG+EAKDL
Sbjct: 426 RFLDSDVDYKGLDFEFIPFGTGRRICPGIAMGIATVELALAQILHSFDWELPSGVEAKDL 485

BLAST of Cla97C03G054280 vs. NCBI nr
Match: XP_011651857.2 (cytochrome P450 71A1 [Cucumis sativus] >KAE8652610.1 hypothetical protein Csa_013309 [Cucumis sativus])

HSP 1 Score: 513.1 bits (1320), Expect = 1.9e-141
Identity = 270/379 (71.24%), Postives = 296/379 (78.10%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           +VFSPYGS+WRQ+RKICI++LLSAKRVQSF+ IRQQE ARLV RIS SNN VDLS+L+ L
Sbjct: 132 IVFSPYGSYWRQVRKICILQLLSAKRVQSFSLIRQQEVARLVDRISHSNNRVDLSNLLGL 191

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCR ALGREFSAGGEYHLHGIQ LLEEYQIL+                   G +S
Sbjct: 192 YANDVLCRSALGREFSAGGEYHLHGIQKLLEEYQILLGGFCIGDLFPSLAFLSNFTGMRS 251

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RL RT   FDKLFDQVIAEHL+P+REKL ESKDLVDVLL+IQ+NGSDDK PLTMDNVK I
Sbjct: 252 RLARTANGFDKLFDQVIAEHLNPEREKLEESKDLVDVLLEIQKNGSDDKVPLTMDNVKAI 311

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMF AGTDTTFI LDWGMTEL+THPKAMKRAQ EIR +VG+RR V+ESD+ EM YLKA
Sbjct: 312 ILDMFAAGTDTTFIALDWGMTELITHPKAMKRAQSEIRRVVGDRRNVTESDVLEMPYLKA 371

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPPAPV VPRETMED R        KT                   +    +
Sbjct: 372 VVKEVLRLHPPAPVLVPRETMEDVRIEGYDIPAKTRVFVNVWGIGRDPESWKDPESFEPE 431

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+   VDY GLDFEF+P G GRRICPGI+MGIVTIELALAQILHSFDWELP+GIEAKDL
Sbjct: 432 RFLGSGVDYGGLDFEFLPFGGGRRICPGITMGIVTIELALAQILHSFDWELPNGIEAKDL 491

BLAST of Cla97C03G054280 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 7.4e-112
Identity = 215/380 (56.58%), Postives = 265/380 (69.74%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQ-SNNPVDLSSLVA 60
           +VFSPYG++WR IRKICI+ELLSAKRVQSF+H+R++E ARLV R+++      +L+ ++ 
Sbjct: 131 VVFSPYGAYWRHIRKICILELLSAKRVQSFSHVREEEVARLVRRVAEFYPGTTNLTKMLG 190

Query: 61  LYANEVLCRMALGREFSAGGEYHLHGIQALLEEYQ-------------------ILVGTK 120
           LYAN+VLCR+A GR FS GG+Y  HG Q +LEEYQ                    L G K
Sbjct: 191 LYANDVLCRVAFGRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMK 250

Query: 121 SRLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKG 180
           SRL  TF+ FD+LFDQ++ +HLSP+REK  E KDLVDVLLDIQ+  S +  PLTMDNVK 
Sbjct: 251 SRLQETFRRFDELFDQMVTDHLSPKREK-EEHKDLVDVLLDIQKKESTE-MPLTMDNVKA 310

Query: 181 IILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLK 240
           IILDMF AGTDTTFI LDWGMTELL + K ++RAQ E+R +VGERR+V ESDL ++ Y+K
Sbjct: 311 IILDMFAAGTDTTFITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMK 370

Query: 241 AVVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSS 300
           AV+KE+ RLHPPAPV VPRE+MED          KT                   +    
Sbjct: 371 AVIKEIFRLHPPAPVLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEP 430

Query: 301 QRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKD 335
           +RF+   +D++G DFE IP GAGRR CP ++ G  TIELALAQ+LH+FDWELP    AKD
Sbjct: 431 ERFIGSTIDFKGQDFELIPFGAGRRGCPAVTFGTATIELALAQLLHTFDWELPLDTAAKD 490

BLAST of Cla97C03G054280 vs. ExPASy Swiss-Prot
Match: O81970 (Cytochrome P450 71A9 OS=Glycine max OX=3847 GN=CYP71A9 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-76
Identity = 155/376 (41.22%), Postives = 231/376 (61.44%), Query Frame = 0

Query: 3   FSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVALYA 62
           F+PYG +WR++RKI I+ELLS KRVQSF  +R +E   L+  I+ S+ PV+LS L     
Sbjct: 117 FAPYGEYWREMRKIMILELLSPKRVQSFEAVRFEEVKLLLQTIALSHGPVNLSELTLSLT 176

Query: 63  NEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKSRL 122
           N ++CR+ALG+   +G +   + +  +L+E Q ++                   G ++RL
Sbjct: 177 NNIVCRIALGKRNRSGAD-DANKVSEMLKETQAMLGGFFPVDFFPRLGWLNKFSGLENRL 236

Query: 123 VRTFKAFDKLFDQVIAEHLSP-QREKLG-ESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 182
            + F+  D  +DQVI EH++    E+ G E +D+VDVLL +Q++  +    +T D +KG+
Sbjct: 237 EKIFREMDNFYDQVIKEHIADNSSERSGAEHEDVVDVLLRVQKD-PNQAIAITDDQIKGV 296

Query: 183 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 242
           ++D+FVAGTDT    + W M+EL+ +PKAMKRAQ+E+R +V  + +V E DLS++LY+K+
Sbjct: 297 LVDIFVAGTDTASATIIWIMSELIRNPKAMKRAQEEVRDLVTGKEMVEEIDLSKLLYIKS 356

Query: 243 VVKEVLRLHPPAPVSVPRETMEDNRGKTQKVSS--------------------------Q 302
           VVKEVLRLHPPAP+ VPRE  E+   K  ++ +                          +
Sbjct: 357 VVKEVLRLHPPAPLLVPREITENCTIKGFEIPAKTRVLVNAKSIAMDPCCWENPNEFLPE 416

Query: 303 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 332
           RF+   +D++G  FE +P G GRR CPG++  +  +ELALA +L  FDWELP G+  +DL
Sbjct: 417 RFLVSPIDFKGQHFEMLPFGVGRRGCPGVNFAMPVVELALANLLFRFDWELPLGLGIQDL 476

BLAST of Cla97C03G054280 vs. ExPASy Swiss-Prot
Match: A0A0N9HT29 (Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71BE54 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 6.1e-74
Identity = 144/375 (38.40%), Postives = 223/375 (59.47%), Query Frame = 0

Query: 4   SPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVALYAN 63
           +PYG +WRQIRKIC++ELLSAKRVQSF  +R++E + L+  IS  ++ V+ S ++    N
Sbjct: 120 APYGDYWRQIRKICVLELLSAKRVQSFQTVREEEISNLIRSISAESSKVNFSEMITSLTN 179

Query: 64  EVLCRMALGRE--------------FSAGGEYHLHGIQALLEEYQILVGTKSRLVRTFKA 123
           +++ R A G +                  G + + G+   L+   ++ G K +L R    
Sbjct: 180 DIIARAAFGEKCKDKHAFLSLIEEGIQLAGGFDIAGLFPSLKLLHVITGKKRQLERVHNE 239

Query: 124 FDKLFDQVIAEHLSPQR-EKLGESK---DLVDVLLDIQRNGSDDKFPLTMDNVKGIILDM 183
            D++   +I E+   +R  KL E K   +LVDVL+ +Q N +  + PL  DN+K +ILD+
Sbjct: 240 LDRILVDIIKENKEKRRASKLNEDKYEENLVDVLVRVQEN-TQLEVPLANDNLKAVILDI 299

Query: 184 FVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKAVVKE 243
           FVAG++T+   ++W M+E+L +PK MK+AQ E+R +    + ++E+++ E+ YLK V+KE
Sbjct: 300 FVAGSETSSTTIEWAMSEMLKNPKVMKKAQAEVRRVFSGNKKINETEIQELSYLKLVLKE 359

Query: 244 VLRLHPPAPVSVPRETME--------------------------DNRGKTQKVSSQRFME 303
            LRLH PAP+ +PRE  E                          +N     +   +RF  
Sbjct: 360 TLRLHAPAPLLIPRECRESCEIDGYEIPKKTMVMVNAWAIGRDPENWSNGDRFEPERFDG 419

Query: 304 IEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDLDMTE 335
           I VDY+G +FE+IP GAGRRICPG+  G+  +E+ LA++L+ FDWELP+GI+ ++LDM E
Sbjct: 420 ISVDYKGTNFEYIPFGAGRRICPGMLFGMANVEVPLARLLYHFDWELPNGIKPEELDMDE 479

BLAST of Cla97C03G054280 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-72
Identity = 153/380 (40.26%), Postives = 216/380 (56.84%), Query Frame = 0

Query: 4   SPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPV-DLSSLVALYA 63
           +PYG +WRQ+R IC++ LLS +RV+SF  +R++E   ++  I  S++ V +LS +     
Sbjct: 135 APYGEYWRQVRSICVLNLLSNRRVRSFRSVREEETKSMIRNIKGSSSSVLNLSEMFVRLT 194

Query: 64  NEVLCRMALGREFSAG-GEYHLHGIQALLEEYQILVGT-------------------KSR 123
           N+V+C++ALGR++S G G       + +L E+  L+GT                    ++
Sbjct: 195 NDVVCKVALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAK 254

Query: 124 LVRTFKAFDKLFDQVIAEHLS-PQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 183
           L +  K  D   D V+ EH++   R    + KD +D+LL IQ+  S    P+   +VKGI
Sbjct: 255 LDKVAKQLDDFIDTVVQEHMNHSSRSGDDDQKDFLDILLAIQKETSAG-IPIDGVSVKGI 314

Query: 184 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERR-IVSESDLSEMLYLK 243
           ILDMF AGTDTT+  L+W MTELL HP+ M + Q+E+R IVG R  +++E DL EM YLK
Sbjct: 315 ILDMFAAGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVEMHYLK 374

Query: 244 AVVKEVLRLHPPAPVSVPRETMEDNR--------------------------GKTQKVSS 303
           AV KE LRLHPP P+ VPR +  D                             K ++   
Sbjct: 375 AVTKETLRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEP 434

Query: 304 QRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKD 335
           +RF+   +DY+G DFE IP GAGRR+CPGI   +   E+ALA I+H FDW LP     +D
Sbjct: 435 ERFLNNGIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALPDEASGED 494

BLAST of Cla97C03G054280 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.7e-72
Identity = 154/383 (40.21%), Postives = 223/383 (58.22%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRI-----SQSNNPVDLS 60
           + F+PYG +WRQ+R+IC++ELLS KRV  F + R +E A LV +I     S +  P++L 
Sbjct: 139 IAFAPYGEYWRQVRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKASASANGAPINLG 198

Query: 61  SLVALYANEVLCRMALGREFSAG-----GE-----------YHLHGIQALLEEYQILVGT 120
            L+   +N ++CR  LG++F        GE           +        L+      G 
Sbjct: 199 ELLVSTSNNIICRCILGQKFEDKEDNWFGETTKELMTQVMSFSFGDFFPSLKWIDRARGY 258

Query: 121 KSRLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVK 180
            + L   +  FDK FD++I EH + Q+E     KD+VD+LLD+Q +GS D F LT  NVK
Sbjct: 259 LAYLKSIWLEFDKFFDKLIDEHKAAQKEGKPRKKDIVDILLDVQNDGSLD-FELTTSNVK 318

Query: 181 GIILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYL 240
            I+ DMFV G+DT++    W M+EL  +P+ MK+ Q+E+R + G+R  V ESD++EM YL
Sbjct: 319 AILQDMFVGGSDTSWTAAIWLMSELSQNPRVMKKVQEEVRRVAGKRGYVEESDINEMKYL 378

Query: 241 KAVVKEVLRLHPPAPVSVPRETMEDNR-------GKTQ-------------------KVS 300
             V+KE LRLHPPAP+ +PRE M D +        KTQ                   +  
Sbjct: 379 TCVIKENLRLHPPAPLLLPREAMSDVKLGGFDIPAKTQVFVNAYAVQRDPKVWDKPDEFM 438

Query: 301 SQRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIE-- 335
            +RF E  V + G DFE IP GAGRR+CPG++ G+ + +  LA +L+ FDW+LPSG    
Sbjct: 439 PERFEENNVGFVGQDFELIPFGAGRRVCPGLAFGVASAQYVLANMLYWFDWKLPSGGSKL 498

BLAST of Cla97C03G054280 vs. ExPASy TrEMBL
Match: A0A5D3BLJ1 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004150 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 5.3e-145
Identity = 272/379 (71.77%), Postives = 302/379 (79.68%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WRQ+RKICI++LL+AKRVQSFA IRQQE +RLV+RIS S NPVDL++L+A+
Sbjct: 122 IAFSPYGSYWRQVRKICILQLLNAKRVQSFAFIRQQEVSRLVNRISHSTNPVDLTNLLAM 181

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCRMALGREFS GGEYHLHGIQ LLEEYQIL+                   GTKS
Sbjct: 182 YANDVLCRMALGREFSTGGEYHLHGIQELLEEYQILLGGFFFADLFPSLSFISTLTGTKS 241

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLV+TFKAFDKL DQVIAEH S  REKLGESKDLVDVLLDIQ+NG +DKF LTMDNVKGI
Sbjct: 242 RLVKTFKAFDKLVDQVIAEHQSHDREKLGESKDLVDVLLDIQKNGFEDKFLLTMDNVKGI 301

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMFVAGTDTTFI LDWGMTEL+THP  MKRAQ EIR +VG+RR V+ESDL EM YLKA
Sbjct: 302 ILDMFVAGTDTTFIALDWGMTELITHPNVMKRAQSEIRRVVGDRRNVTESDLLEMPYLKA 361

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPP PVSVPRET ED +        KT                   +    +
Sbjct: 362 VVKEVLRLHPPVPVSVPRETTEDVKIEGYDIPAKTRVFVNIWAIGRDPESWKDPETFEPE 421

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDY+GL+FEFIP GAGRRICPGI+MGIVTIELALAQILHSFDWELP G+EAKDL
Sbjct: 422 RFLESEVDYKGLNFEFIPFGAGRRICPGIAMGIVTIELALAQILHSFDWELPKGMEAKDL 481

BLAST of Cla97C03G054280 vs. ExPASy TrEMBL
Match: A0A5D3CT71 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00600 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 6.0e-141
Identity = 261/379 (68.87%), Postives = 297/379 (78.36%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WR++RKICI++LLS KRVQSFA IRQQE ARLV+RIS SNNPVDLS+L+AL
Sbjct: 123 IAFSPYGSYWRRVRKICILQLLSTKRVQSFAFIRQQEVARLVNRISHSNNPVDLSNLLAL 182

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           Y N+V+CRMALGREFS GG YHLHGIQ LLEEYQ+L+                   G KS
Sbjct: 183 YGNDVICRMALGREFSVGGAYHLHGIQELLEEYQLLLRGFCFGDLFPSLSFISKLTGMKS 242

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLVRTFK FDKL DQVI EH SP+ EKLGESKDLVDVLLDIQ+NGSDD FPLTMDNVK I
Sbjct: 243 RLVRTFKRFDKLLDQVIVEHQSPEGEKLGESKDLVDVLLDIQKNGSDDGFPLTMDNVKAI 302

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           +LDMF+AGT+TTF  LDWGMTEL+THPKAM+RAQ+EIRSIVGER+IV+E D+ EM YLKA
Sbjct: 303 LLDMFIAGTETTFTALDWGMTELITHPKAMERAQNEIRSIVGERKIVTEGDVLEMHYLKA 362

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPPAP+++PRET +D R        KT                   +    +
Sbjct: 363 VVKEVLRLHPPAPLALPRETTDDVRIEGYDIPAKTRVFVNVWGIGRDPESWKNPESFEPE 422

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDYRGLDFEFIP G GRRICPGI+MGI  IE+A AQILHSFDWELPSGIE +DL
Sbjct: 423 RFIENEVDYRGLDFEFIPFGGGRRICPGITMGIAMIEIAFAQILHSFDWELPSGIEVEDL 482

BLAST of Cla97C03G054280 vs. ExPASy TrEMBL
Match: A0A5A7VFB1 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold538G00670 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 6.0e-141
Identity = 261/379 (68.87%), Postives = 297/379 (78.36%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WR++RKICI++LLS KRVQSFA IRQQE ARLV+RIS SNNPVDLS+L+AL
Sbjct: 123 IAFSPYGSYWRRVRKICILQLLSTKRVQSFAFIRQQEVARLVNRISHSNNPVDLSNLLAL 182

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           Y N+V+CRMALGREFS GG YHLHGIQ LLEEYQ+L+                   G KS
Sbjct: 183 YGNDVICRMALGREFSVGGAYHLHGIQELLEEYQLLLRGFCFGDLFPSLSFISKLTGMKS 242

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLVRTFK FDKL DQVI EH SP+ EKLGESKDLVDVLLDIQ+NGSDD FPLTMDNVK I
Sbjct: 243 RLVRTFKRFDKLLDQVIVEHQSPEGEKLGESKDLVDVLLDIQKNGSDDGFPLTMDNVKAI 302

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           +LDMF+AGT+TTF  LDWGMTEL+THPKAM+RAQ+EIRSIVGER+IV+E D+ EM YLKA
Sbjct: 303 LLDMFIAGTETTFTALDWGMTELITHPKAMERAQNEIRSIVGERKIVTEGDVLEMHYLKA 362

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPPAP+++PRET +D R        KT                   +    +
Sbjct: 363 VVKEVLRLHPPAPLALPRETTDDVRIEGYDIPAKTRVFVNVWGIGRDPESWKNPESFEPE 422

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDYRGLDFEFIP G GRRICPGI+MGI  IE+A AQILHSFDWELPSGIE +DL
Sbjct: 423 RFIENEVDYRGLDFEFIPFGGGRRICPGITMGIAMIEIAFAQILHSFDWELPSGIEVEDL 482

BLAST of Cla97C03G054280 vs. ExPASy TrEMBL
Match: A0A1S3CHD8 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103500475 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 6.0e-141
Identity = 261/379 (68.87%), Postives = 297/379 (78.36%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           + FSPYGS+WR++RKICI++LLS KRVQSFA IRQQE ARLV+RIS SNNPVDLS+L+AL
Sbjct: 123 IAFSPYGSYWRRVRKICILQLLSTKRVQSFAFIRQQEVARLVNRISHSNNPVDLSNLLAL 182

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           Y N+V+CRMALGREFS GG YHLHGIQ LLEEYQ+L+                   G KS
Sbjct: 183 YGNDVICRMALGREFSVGGAYHLHGIQELLEEYQLLLRGFCFGDLFPSLSFISKLTGMKS 242

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLVRTFK FDKL DQVI EH SP+ EKLGESKDLVDVLLDIQ+NGSDD FPLTMDNVK I
Sbjct: 243 RLVRTFKRFDKLLDQVIVEHQSPEGEKLGESKDLVDVLLDIQKNGSDDGFPLTMDNVKAI 302

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           +LDMF+AGT+TTF  LDWGMTEL+THPKAM+RAQ+EIRSIVGER+IV+E D+ EM YLKA
Sbjct: 303 LLDMFIAGTETTFTALDWGMTELITHPKAMERAQNEIRSIVGERKIVTEGDVLEMHYLKA 362

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPPAP+++PRET +D R        KT                   +    +
Sbjct: 363 VVKEVLRLHPPAPLALPRETTDDVRIEGYDIPAKTRVFVNVWGIGRDPESWKNPESFEPE 422

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+E EVDYRGLDFEFIP G GRRICPGI+MGI  IE+A AQILHSFDWELPSGIE +DL
Sbjct: 423 RFIENEVDYRGLDFEFIPFGGGRRICPGITMGIAMIEIAFAQILHSFDWELPSGIEVEDL 482

BLAST of Cla97C03G054280 vs. ExPASy TrEMBL
Match: A0A5D3BIU1 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004130 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.3e-140
Identity = 268/379 (70.71%), Postives = 294/379 (77.57%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNNPVDLSSLVAL 60
           +VFSPYGS+WRQ+RKICI++LLSA+RVQSF+ IRQQE ARLV RIS S + V+LSSL+ L
Sbjct: 123 IVFSPYGSYWRQVRKICILQLLSARRVQSFSLIRQQEVARLVDRISHSKDRVNLSSLLGL 182

Query: 61  YANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV-------------------GTKS 120
           YAN+VLCR ALGREFSAGGEYHLHG Q LLEEYQIL+                   G KS
Sbjct: 183 YANDVLCRAALGREFSAGGEYHLHGFQKLLEEYQILLGGFCIGDLFPSLAFLSNFTGMKS 242

Query: 121 RLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGI 180
           RLVRT   FDKLFDQVIAEHL P+REKL ES+DLVD+LL+IQ+NGSDDK PLTMDNVK I
Sbjct: 243 RLVRTANGFDKLFDQVIAEHLDPEREKLEESQDLVDILLEIQKNGSDDKIPLTMDNVKAI 302

Query: 181 ILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKA 240
           ILDMF AGTDTTFI LDWGMTEL+THP AMKRAQ EIR +VG+RR V+ESDL EM YLKA
Sbjct: 303 ILDMFAAGTDTTFIALDWGMTELITHPNAMKRAQSEIRRVVGDRRNVTESDLLEMPYLKA 362

Query: 241 VVKEVLRLHPPAPVSVPRETMEDNR-------GKT-------------------QKVSSQ 300
           VVKEVLRLHPPAPV VPRET ED R        KT                   +    +
Sbjct: 363 VVKEVLRLHPPAPVLVPRETTEDVRIEGYDIPAKTRVFVNAWGIGRDPESWKDPETFEPE 422

Query: 301 RFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDL 335
           RF+  EVDY GLDFEFIP GAGRRICPGI+MGI TIELALAQILHSFDWELP+GIEAKDL
Sbjct: 423 RFLGSEVDYGGLDFEFIPFGAGRRICPGITMGIATIELALAQILHSFDWELPNGIEAKDL 482

BLAST of Cla97C03G054280 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 256.5 bits (654), Expect = 3.0e-68
Identity = 143/382 (37.43%), Postives = 219/382 (57.33%), Query Frame = 0

Query: 3   FSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSN---NPVDLSSLVA 62
           F+PYG  WR +RK+ +IEL S K+  SF +IR++E   LV ++S+++   +PV+L   + 
Sbjct: 116 FAPYGEEWRALRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALF 175

Query: 63  LYANEVLCRMALGREFSAG---GEYHLHGIQALLEEYQI----------------LVGTK 122
             +  ++CR+A G+         E  +  + +  E+ Q                 + G  
Sbjct: 176 TLSASIVCRLAFGQNLHESEFIDEDSMEDLASRSEKIQAKFAFSNFFPGGWILDKITGQS 235

Query: 123 SRLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDD-KFPLTMDNVK 182
             L   F   D  F+QV+ +HL P R+ L E+ D+VDV++D+    S D  F LT D++K
Sbjct: 236 KSLNEIFADLDGFFNQVLDDHLKPGRKVL-ETPDVVDVMIDMMNKQSQDGSFKLTTDHIK 295

Query: 183 GIILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERR-IVSESDLSEMLY 242
           GII D+F+AG +T+   + W MTEL+ +P+ MK+ QDE+R+++GE+R  ++E DL+++ Y
Sbjct: 296 GIISDIFLAGVNTSATTILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNY 355

Query: 243 LKAVVKEVLRLHPPAPVSVPRETME-------DNRGKTQ-------------------KV 302
            K V+KE  RLHP AP+ +PRE M        D   KTQ                   + 
Sbjct: 356 FKLVIKETFRLHPAAPLLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEF 415

Query: 303 SSQRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEA 335
             +RF++  VDYRGL+FE +P G+GRRICPG++MGI T+EL L  +L+ FDW LP G   
Sbjct: 416 KPERFVDSSVDYRGLNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRTV 475

BLAST of Cla97C03G054280 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 253.4 bits (646), Expect = 2.6e-67
Identity = 140/390 (35.90%), Postives = 222/390 (56.92%), Query Frame = 0

Query: 1   MVFSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQS---NNPVDLSSL 60
           + F+PYG +WR++RK+ +IEL S+K+VQSF +IR++E   +V ++S+S    +PVDLS  
Sbjct: 114 ITFAPYGEYWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKT 173

Query: 61  VALYANEVLCRMALGREFSAGG-EYHLHGIQALLEEYQILVGT----------------- 120
                  ++CR+ALG+ F+  G       I+ L+ E    +GT                 
Sbjct: 174 FFSLTASIICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDW 233

Query: 121 ----KSRLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLD-IQRNGSDDKFPLT 180
                 ++ + FK  D  +  VI +HL P+  K   ++D+V ++LD I +    D F L 
Sbjct: 234 LFQRHKKINKVFKELDAFYQHVIDDHLKPEGRK---NQDIVTLILDMIDKQEDSDSFKLN 293

Query: 181 MDNVKGIILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVG-ERRIVSESDL 240
           MDN+K I++D+F+AG DT+ + + W MTEL+ +P+ MK+AQ+ IR+ +G ++  ++E DL
Sbjct: 294 MDNLKAIVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDL 353

Query: 241 SEMLYLKAVVKEVLRLHPPAPVSVPRETME-------DNRGKTQ---------------- 300
            ++ YL  ++KE  RLHP  P  VPRETM        D   KTQ                
Sbjct: 354 GKVEYLNHILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWN 413

Query: 301 ---KVSSQRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELP 338
              + + +RF    VD+RG  F+ +P G+GRRICPG+ M I ++ELAL  +L+ FDW +P
Sbjct: 414 DPEEFNPERFANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMP 473

BLAST of Cla97C03G054280 vs. TAIR 10
Match: AT1G13110.1 (cytochrome P450, family 71 subfamily B, polypeptide 7 )

HSP 1 Score: 252.7 bits (644), Expect = 4.4e-67
Identity = 137/386 (35.49%), Postives = 220/386 (56.99%), Query Frame = 0

Query: 3   FSPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQS---NNPVDLSSLVA 62
           F+PYG  W+ +RK+ ++ELL+ K+ QSF +IR++E   L+ ++++S    +PV+L   + 
Sbjct: 117 FAPYGEEWKALRKLVVMELLNTKKFQSFRYIREEENDLLIKKLTESALKKSPVNLKKTLF 176

Query: 63  LYANEVLCRMALGREFSAGGEYHLHGIQALLEEYQILV--------------------GT 122
                ++CR+A G             +  L+ ++++LV                    G 
Sbjct: 177 TLVASIVCRLAFGVNIHKCEFVDEDNVADLVNKFEMLVAGVAFTDFFPGVGWLVDRISGQ 236

Query: 123 KSRLVRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDD--KFPLTMDN 182
              L   F   D  F  V+ +H+ P R+ + E+ D+VDV+LD+ +    D   F LT D+
Sbjct: 237 NKTLNNVFSELDTFFQNVLDDHIKPGRQ-VSENPDVVDVMLDLMKKQEKDGESFKLTTDH 296

Query: 183 VKGIILDMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERR-IVSESDLSEM 242
           +KGII D+F+AG +T+ + L+W M EL+ +P+ MK+ QDEIR+ +G+++  ++E DLS++
Sbjct: 297 LKGIISDIFLAGVNTSAVTLNWAMAELIRNPRVMKKVQDEIRTTLGDKKQRITEQDLSQV 356

Query: 243 LYLKAVVKEVLRLHPPAPVSVPRETME-------DNRGKTQ------------------- 302
            Y K VVKE+ RLHP AP+ +PRETM        D   KTQ                   
Sbjct: 357 HYFKLVVKEIFRLHPAAPLLLPRETMSHVKIQGYDIPVKTQMMINIYSIARDPKLWTNPD 416

Query: 303 KVSSQRFMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGI 337
           + +  RF++  +DYRGL+FE +P G+GRRICPG+++GI T+EL L  +L+ FDW +P G 
Sbjct: 417 EFNPDRFLDSSIDYRGLNFELLPFGSGRRICPGMTLGITTVELGLLNLLYFFDWVVPVGK 476

BLAST of Cla97C03G054280 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 241.9 bits (616), Expect = 7.7e-64
Identity = 134/371 (36.12%), Postives = 209/371 (56.33%), Query Frame = 0

Query: 4   SPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQS-NNPVDLSSLVALYA 63
           +PYG +WRQ++ +C++ L S K V+SF  +R++E + ++ +I +S + PV+LS ++    
Sbjct: 117 APYGEYWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSLT 176

Query: 64  NEVLCRMALGREFSAGGEYHLHGIQALLEEYQILVGTKS-------------------RL 123
           N+V+C++ALGR++  GGE      + L+E    L+GT S                   +L
Sbjct: 177 NDVICKVALGRKY--GGE---TDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQL 236

Query: 124 VRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGIIL 183
            +T    DK F++V+ +H+   R    +  D VDVLL IQR+ +   F +   ++K I++
Sbjct: 237 EKTANDVDKFFERVVQDHVDGNR----DMTDFVDVLLAIQRDKTVG-FEINRVSIKAIVM 296

Query: 184 DMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKAVV 243
           ++FV GTDT+  +++W MTELL HPK +KR Q+E+R+I  ++  VSE ++  M YLKAV+
Sbjct: 297 NVFVGGTDTSSTLMEWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVI 356

Query: 244 KEVLRLHPPAPVSVPRETMEDNR---------------------------GKTQKVSSQR 303
           KE LRLHPP P+ VP E+ +D R                              ++   +R
Sbjct: 357 KEALRLHPPLPLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPER 416

Query: 304 FMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDLD 328
            ++  VDYRG  FE IP G+GRRICP IS  +V  E+ LA ++H FDW L         +
Sbjct: 417 HLDSSVDYRGQAFELIPFGSGRRICPAISFAVVLNEVVLANLVHRFDWRLSVESTEDQTE 476

BLAST of Cla97C03G054280 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 135/378 (35.71%), Postives = 211/378 (55.82%), Query Frame = 0

Query: 4   SPYGSHWRQIRKICIIELLSAKRVQSFAHIRQQEAARLVHRISQSNN-PVDLSSLVALYA 63
           +PYG +WRQ++ +C+I LLS K V+SF  +R++E   ++ +I +S++ P ++S ++    
Sbjct: 116 APYGEYWRQMKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLT 175

Query: 64  NEVLCRMALGREFSAGGEYHLHGIQALLEEYQILVGTKS-------------------RL 123
           N+V+CR+ALGR++  GGE     +   L E   L+GT S                   +L
Sbjct: 176 NDVICRVALGRKY--GGETDFKKLTDRLSE---LLGTFSIGSFVPWLAWVDWIRGWDAQL 235

Query: 124 VRTFKAFDKLFDQVIAEHLSPQREKLGESKDLVDVLLDIQRNGSDDKFPLTMDNVKGIIL 183
            +  K  D  F++V+ +H    R    +  DL+D LL ++R  S   F +   ++K I L
Sbjct: 236 DKMGKDLDDFFEKVVQDHEDGDRR---DGTDLIDALLRVKREKSPG-FEIERVSIKAITL 295

Query: 184 DMFVAGTDTTFIVLDWGMTELLTHPKAMKRAQDEIRSIVGERRIVSESDLSEMLYLKAVV 243
           D+FV G+DT+F +L+W MTELL HPK++ R Q+E+R+I   +  VSE D+  M YLKAV+
Sbjct: 296 DVFVGGSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVI 355

Query: 244 KEVLRLHPPAPVSVPRETMEDNRGK---------------------------TQKVSSQR 303
           KE LRLHPP P+  P E+ ED + +                            ++   +R
Sbjct: 356 KEALRLHPPFPMMAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPER 415

Query: 304 FMEIEVDYRGLDFEFIPLGAGRRICPGISMGIVTIELALAQILHSFDWELPSGIEAKDLD 335
            ++  VD+RG +FE +P GAGRRICP +S  +V  E+ LA ++H FDW+LP   +    D
Sbjct: 416 HLDTSVDFRGQNFELLPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDKTD 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYJ99205.11.1e-14471.77cytochrome P450 71A1-like [Cucumis melo var. makuwa][more]
XP_031738204.12.7e-14370.98cytochrome P450 71A1-like [Cucumis sativus] >KAE8652612.1 hypothetical protein C... [more]
XP_031743079.13.5e-14370.71cytochrome P450 71A1-like [Cucumis sativus] >KAE8652614.1 hypothetical protein C... [more]
XP_038895182.13.0e-14271.24cytochrome P450 71AP13-like [Benincasa hispida][more]
XP_011651857.21.9e-14171.24cytochrome P450 71A1 [Cucumis sativus] >KAE8652610.1 hypothetical protein Csa_01... [more]
Match NameE-valueIdentityDescription
A0A068Q7217.4e-11256.58Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
O819701.7e-7641.22Cytochrome P450 71A9 OS=Glycine max OX=3847 GN=CYP71A9 PE=2 SV=1[more]
A0A0N9HT296.1e-7438.40Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 G... [more]
A0A068Q6L22.0e-7240.26Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
A0A068Q6095.7e-7240.21Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP... [more]
Match NameE-valueIdentityDescription
A0A5D3BLJ15.3e-14571.77Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5D3CT716.0e-14168.87Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7VFB16.0e-14168.87Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S3CHD86.0e-14168.87cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103500475 PE=3 SV=1[more]
A0A5D3BIU11.3e-14070.71Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT3G26210.13.0e-6837.43cytochrome P450, family 71, subfamily B, polypeptide 23 [more]
AT1G13080.12.6e-6735.90cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
AT1G13110.14.4e-6735.49cytochrome P450, family 71 subfamily B, polypeptide 7 [more]
AT3G48270.17.7e-6436.12cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT3G48280.11.3e-6335.71cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 272..281
score: 50.93
coord: 221..232
score: 48.01
coord: 168..185
score: 38.4
IPR001128Cytochrome P450PFAMPF00067p450coord: 254..328
e-value: 9.7E-11
score: 41.1
coord: 3..244
e-value: 1.4E-43
score: 149.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 281..304
score: 27.7
coord: 271..281
score: 51.47
coord: 157..174
score: 39.99
coord: 177..203
score: 32.84
coord: 61..79
score: 16.66
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 254..334
e-value: 1.0E-13
score: 53.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..252
e-value: 3.8E-56
score: 192.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..335
NoneNo IPR availablePANTHERPTHR24298:SF701CYTOCHROME P450 FAMILY 71 PROTEINcoord: 2..244
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 251..335
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 2..244
NoneNo IPR availablePANTHERPTHR24298:SF701CYTOCHROME P450 FAMILY 71 PROTEINcoord: 251..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C03G054280.1Cla97C03G054280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen