ClCG11G005640 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG11G005640
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionUbiquinol-cytochrome c reductase complex 6.7 kDa
LocationCG_Chr11: 6135489 .. 6139551 (-)
RNA-Seq ExpressionClCG11G005640
SyntenyClCG11G005640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGTTGATATGGTGGCTGGAACCGAATCCATTGTATAAAAACTTCCTAATTTTTGTATTAGGTTTGTCATTGGATTTGGAAGACGAATTGAGAGAAAAAAAATGGCAGGTGAATCGACTTTGTTCAAGTTCTTGAGCCCAGGACGCCGTTTCCAATCTACGGACATCCAAGCCGCTGCCGGCTGGGGTATCGCCGCCGCCACTACTGCTCTGTGGGTCGTTCAGGTTTTCTCCTTTCCTAAACTCATCGATCTATACTATTATTTATGGTTTTCGGAGATTTATACAGTGAATCTTTTGACTCTCTATGTTAGATTAAAGGATGCATCGATCTTCTTGAAACTTGATTCCTGATTCTGCTGTCTTACGTATACCGTCTTTATGATTTCTGTTTCTTGTCTTGGACGATTTACCTTGGCGTATTCGGATTTCTATTTTTACTCCAGCGTTAATATAGTGATTACGGGATTAGCTTTCATTTTCAGTTTGTCATTCGCAATAGAGATCTGAAAGAAATAAATTCCTATGGATTATGGTGTCTATGGATATACGTATTCCTCGTTGATTAGGAGCAGAGAGATTTATTTTTATCTGTTTTCTTCTTTTTGGTATGTCATCGAGCACAAGATGATACTCTTGCCCAGTTTTGTATGATAACTAGTAGAATTGTAGAAATATTAAGAGATGTAGTGCACTAAGATTTTTTACACTCTACATATTTCTAGAACTCAAGGATACAACCTTTTGAACAAAGGTAAGGCTATTTAGACATTGAATCTCACAAGTTTCTAAAAATTTGTAGTAGTTCATTATATATGGCTCCACTTCCTGCCGTCCTCATGATCACACTCTGGTTCCACATTGTAAGTTTGTTTTACTTCCAATTCCAATCCAAGTTCAGTTTCAGTATGGAGCTTTTAGCCCCTCACAGAGACCTAAGGCCTTACCTGAGCGAAAGTTTTGATCCTCTACCCTTTCTGTTTATACAGTCTTTTCCAAATTCCATAATTGTATCACTATAGTTGGCTTCATTTGTTAGTTTTAAACCATTAGTTAACTTTCTACCGGTACCTGCTGCAAAATTTCCATCGACGCCACCAACCAAAAGCCTAATCCTAACTGCCATCAATACTCTCAAACAGTCCTGAGATAATGTCAAAAACTTTGTTGTTTGCACTTTTTACCTGAGGTTCCGTCCATCAGGCTTTCCATCAAAACTCACAACTGATATCTTGGAACTCCTAGGATCTGGCATTCATTTTGCAGTGAAGTATTGTTATGCTCAAAACATATCCTCCTCTCATTGCAACTATTTATGGAAAGACCTCTCACAATAGTCAATGTTTCTTTCTAGTACAAGCCACTCCATAGAGTTACGACCACATTGCAATTCTACTATTACCAAATTCGGATTTGATCTCCGTTCTATCAAACACGTTCTTTGCACTTCCTTCAAGGAATCCCCAAAATTGGAGTGCTCTAATGCACAAGCTAATGGATCCCATGTAATTTTATTTAACAAATTTGATTGATGGTATGGGGTTTTATTAGCTCTATTCCTCTTTCTCTGCTCCTCATGTTCTTTTTCTATATGCTTAGTAAACGTTCTCAACTCCCCCTCCTTAACTAACAATAACTACATCGTTGCCTCTCCTTTTCTACTGATTAGGTTCACTGCTGTTTTTTGTTCCTTTCCCAACTAAACCATAATGGACTTGTTTTCTTTGCTGTGTGTTTTTTCCGATTTATTGCTTGAGAAAAACTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATCTACAAATTTTTGGGTTCCAGATGGGCTCCATCACCTTTGATAGGGAGAATGCATTATCCTATCTTGGTTGGGTTTTTATTAATTCTTATTTTGTGAAGTTTTCTGTATTGCATTGCAAATAAAGGGATATAGATTTGATTATGTTCATTTTCCCTGATTGTTTCGCGAGGCTGCAAACCAACGTAAATTGGTTTGAGTTTTCAAATATTCATTTCTTTGCCTAAACAGTTTGTTCCAATTAATTTTGTACTATATGTTATTTTCTTGGTTAACTAAACGGTGGCCTTTTGTGTTATACTTGTAGAAAATTAACTCTATACAATCTTTCCTCTTTTTGAGGCTCCTCTGTGGTTCACATTAATTATAATGTTTAAACGAATCTAACCATTCCTTTTTCTCTTGTGAACTCTGTGCAGCCATTTGACTGGATCAAGAAGACGTTCTTCGAGAAGCCAGAAGAGGCATGAATTGTTATTTGTGAAGGCTCACCCCAGTTTTTGTTAATAAGTATCAGTTGATTGCCGCAAATGGGTAAACTGCAGAGTTCATTGGAATAACATGTTTTGTAATAGACTCTGTTTTGATTAAATTGAGTTCTTCAACTCTCTCTAACTTTGGAAATTTTATTGTCGAAATTTAAGGTACGTTTGATAAACATTTGATTTTGTATTTTTGGTTTTTGAAAACTAAACTTATAC

mRNA sequence

CGGTTGATATGGTGGCTGGAACCGAATCCATTGTATAAAAACTTCCTAATTTTTGTATTAGGTTTGTCATTGGATTTGGAAGACGAATTGAGAGAAAAAAAATGGCAGGTGAATCGACTTTGTTCAAGTTCTTGAGCCCAGGACGCCGTTTCCAATCTACGGACATCCAAGCCGCTGCCGGCTGGGGTATCGCCGCCGCCACTACTGCTCTGTGGGTCGTTCAGTTCATTATATATGGCTCCACTTCCTGCCGTCCTCATGATCACACTCTGGTTCCACATTGCTTTCCATCAAAACTCACAACTGATATCTTGGAACTCCTAGGATCTGGCATTCATTTTGCAGTGAACCATTTGACTGGATCAAGAAGACGTTCTTCGAGAAGCCAGAAGAGGCATGAATTGTTATTTGTGAAGGCTCACCCCAGTTTTTGTTAATAAGTATCAGTTGATTGCCGCAAATGGGTAAACTGCAGAGTTCATTGGAATAACATGTTTTGTAATAGACTCTGTTTTGATTAAATTGAGTTCTTCAACTCTCTCTAACTTTGGAAATTTTATTGTCGAAATTTAAGGTACGTTTGATAAACATTTGATTTTGTATTTTTGGTTTTTGAAAACTAAACTTATAC

Coding sequence (CDS)

ATGGCAGGTGAATCGACTTTGTTCAAGTTCTTGAGCCCAGGACGCCGTTTCCAATCTACGGACATCCAAGCCGCTGCCGGCTGGGGTATCGCCGCCGCCACTACTGCTCTGTGGGTCGTTCAGTTCATTATATATGGCTCCACTTCCTGCCGTCCTCATGATCACACTCTGGTTCCACATTGCTTTCCATCAAAACTCACAACTGATATCTTGGAACTCCTAGGATCTGGCATTCATTTTGCAGTGAACCATTTGACTGGATCAAGAAGACGTTCTTCGAGAAGCCAGAAGAGGCATGAATTGTTATTTGTGAAGGCTCACCCCAGTTTTTGTTAA

Protein sequence

MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQFIIYGSTSCRPHDHTLVPHCFPSKLTTDILELLGSGIHFAVNHLTGSRRRSSRSQKRHELLFVKAHPSFC
Homology
[Close]
BLAST of ClCG11G005640 vs. NCBI nr
Match: KAG7016049.1 (hypothetical protein SDJN02_21153 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 84.7 bits (208), Expect = 5.4e-13
Identity = 40/41 (97.56%), Postives = 41/41 (100.00%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAGESTLFKFLSPGRRFQSTDIQAAAGWG+AAATTALWVVQ
Sbjct: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGVAAATTALWVVQ 41

[Close]
BLAST of ClCG11G005640 vs. NCBI nr
Match: KAG6578485.1 (hypothetical protein SDJN03_22933, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 84.7 bits (208), Expect = 5.4e-13
Identity = 40/41 (97.56%), Postives = 41/41 (100.00%), Query Frame = 0 

Query: 1   MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
           MAGESTLFKFLSPGRRFQSTDIQAAAGWG+AAATTALWVVQ
Sbjct: 117 MAGESTLFKFLSPGRRFQSTDIQAAAGWGVAAATTALWVVQ 157

[Close]
BLAST of ClCG11G005640 vs. NCBI nr
Match: KGN45840.1 (hypothetical protein Csa_005761 [Cucumis sativus])

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-12
Identity = 38/48 (79.17%), Postives = 43/48 (89.58%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQFIIYGST 49
          MAGES+LFKFLSPGRRFQSTDIQAAAGWG+AAATTALWV+Q   +  +
Sbjct: 1  MAGESSLFKFLSPGRRFQSTDIQAAAGWGVAAATTALWVIQVFTFSKS 48

[Close]
BLAST of ClCG11G005640 vs. NCBI nr
Match: KAA0051979.1 (Ubiquinol-cytochrome c reductase complex 6.7 kDa [Cucumis melo var. makuwa] >TYK04578.1 Ubiquinol-cytochrome c reductase complex 6.7 kDa [Cucumis melo var. makuwa])

HSP 1 Score: 82.8 bits (203), Expect = 2.0e-12
Identity = 38/41 (92.68%), Postives = 41/41 (100.00%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAGES+LFKFLSPGRRFQSTDIQAAAGWG+AAATTALWV+Q
Sbjct: 1  MAGESSLFKFLSPGRRFQSTDIQAAAGWGVAAATTALWVIQ 41

[Close]
BLAST of ClCG11G005640 vs. NCBI nr
Match: KAF3433259.1 (hypothetical protein FNV43_RR24361 [Rhamnella rubrinervis])

HSP 1 Score: 65.9 bits (159), Expect = 2.6e-07
Identity = 31/41 (75.61%), Postives = 35/41 (85.37%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAGE+ LFKFL+P RR QSTDIQAA  WG+AA TTALW+VQ
Sbjct: 23 MAGEAGLFKFLNPRRRPQSTDIQAAGMWGVAATTTALWIVQ 63

[Close]
BLAST of ClCG11G005640 vs. ExPASy Swiss-Prot
Match: P48505 (Ubiquinol-cytochrome c reductase complex 6.7 kDa protein OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 9.2e-08
Identity = 28/40 (70.00%), Postives = 31/40 (77.50%), Query Frame = 0 

Query: 2  AGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          A  + LFKFL P  R QSTDIQAAAGWG+AA T ALWV+Q
Sbjct: 6  AAGNGLFKFLRPKLRPQSTDIQAAAGWGVAAVTGALWVIQ 45

[Close]
BLAST of ClCG11G005640 vs. ExPASy Swiss-Prot
Match: Q94K78 (Cytochrome b-c1 complex subunit 10, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=UCRY PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 5.6e-05
Identity = 25/41 (60.98%), Postives = 29/41 (70.73%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAG S L   + P  + Q+ DIQAAAGWGIAAA  A+WVVQ
Sbjct: 1  MAGTSGLLNAVKP--KIQTIDIQAAAGWGIAAAAGAIWVVQ 39

[Close]
BLAST of ClCG11G005640 vs. ExPASy TrEMBL
Match: A0A0A0K7V0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014600 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 5.8e-13
Identity = 38/48 (79.17%), Postives = 43/48 (89.58%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQFIIYGST 49
          MAGES+LFKFLSPGRRFQSTDIQAAAGWG+AAATTALWV+Q   +  +
Sbjct: 1  MAGESSLFKFLSPGRRFQSTDIQAAAGWGVAAATTALWVIQVFTFSKS 48

[Close]
BLAST of ClCG11G005640 vs. ExPASy TrEMBL
Match: A0A5D3BZX3 (Ubiquinol-cytochrome c reductase complex 6.7 kDa OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002150 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 9.9e-13
Identity = 38/41 (92.68%), Postives = 41/41 (100.00%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAGES+LFKFLSPGRRFQSTDIQAAAGWG+AAATTALWV+Q
Sbjct: 1  MAGESSLFKFLSPGRRFQSTDIQAAAGWGVAAATTALWVIQ 41

[Close]
BLAST of ClCG11G005640 vs. ExPASy TrEMBL
Match: A9PAG4 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_019G059500 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.1e-07
Identity = 30/41 (73.17%), Postives = 34/41 (82.93%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAGE+ +FKFLSP  R QSTDIQ AA WG+AA TTALW+VQ
Sbjct: 1  MAGENAMFKFLSPRLRLQSTDIQTAAFWGVAAGTTALWLVQ 41

[Close]
BLAST of ClCG11G005640 vs. ExPASy TrEMBL
Match: A0A3Q7FPE4 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-06
Identity = 30/40 (75.00%), Postives = 33/40 (82.50%), Query Frame = 0 

Query: 2  AGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          A  + LFKFLSP RR QSTDIQAAAGWG+AA T ALWV+Q
Sbjct: 19 AAGNGLFKFLSPKRRPQSTDIQAAAGWGVAAVTGALWVIQ 58

[Close]
BLAST of ClCG11G005640 vs. ExPASy TrEMBL
Match: A0A2P2J2E2 (Uncharacterized protein OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-06
Identity = 30/41 (73.17%), Postives = 34/41 (82.93%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAGE+ L KFLSP  R QSTDIQAAA WG+AA TTALW++Q
Sbjct: 1  MAGEAALSKFLSPRLRPQSTDIQAAAFWGVAATTTALWLIQ 41

[Close]
BLAST of ClCG11G005640 vs. TAIR 10
Match: AT2G40765.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion, mitochondrial respiratory chain complex III; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 32 Blast hits to 32 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 48.5 bits (114), Expect = 4.0e-06
Identity = 25/41 (60.98%), Postives = 29/41 (70.73%), Query Frame = 0 

Query: 1  MAGESTLFKFLSPGRRFQSTDIQAAAGWGIAAATTALWVVQ 42
          MAG S L   + P  + Q+ DIQAAAGWGIAAA  A+WVVQ
Sbjct: 1  MAGTSGLLNAVKP--KIQTIDIQAAAGWGIAAAAGAIWVVQ 39

The following BLAST results are available for this feature:
BLAST of ClCG11G005640 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7016049.15.4e-1397.56hypothetical protein SDJN02_21153 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6578485.15.4e-1397.56hypothetical protein SDJN03_22933, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN45840.11.2e-1279.17hypothetical protein Csa_005761 [Cucumis sativus][more]
KAA0051979.12.0e-1292.68Ubiquinol-cytochrome c reductase complex 6.7 kDa [Cucumis melo var. makuwa] >TYK... [more]
KAF3433259.12.6e-0775.61hypothetical protein FNV43_RR24361 [Rhamnella rubrinervis][more]
BLAST of ClCG11G005640 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P485059.2e-0870.00Ubiquinol-cytochrome c reductase complex 6.7 kDa protein OS=Solanum tuberosum OX... [more]
Q94K785.6e-0560.98Cytochrome b-c1 complex subunit 10, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
BLAST of ClCG11G005640 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K7V05.8e-1379.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014600 PE=4 SV=1[more]
A0A5D3BZX39.9e-1392.68Ubiquinol-cytochrome c reductase complex 6.7 kDa OS=Cucumis melo var. makuwa OX=... [more]
A9PAG42.1e-0773.17Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_019G059500 PE=2 ... [more]
A0A3Q7FPE41.4e-0675.00Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=4 SV=1[more]
A0A2P2J2E21.4e-0673.17Uncharacterized protein OS=Rhizophora mucronata OX=61149 PE=4 SV=1[more]
BLAST of ClCG11G005640 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G40765.14.0e-0660.98unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG11G005640.1ClCG11G005640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane