ClCG02G006340 (gene) Watermelon (Charleston Gray) v2.5

BLAST of ClCG02G006340 vs. NCBI nr
Match: XP_038888757.1 (RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 867.5 bits (2240), Expect = 6.0e-248
Identity = 426/470 (90.64%), Postives = 438/470 (93.19%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL AL+RRSVILR   SSAAP G SFHFSIFHELKSTLALSNTTE VS +KNEKGSNRW
Sbjct: 1   MVLPALIRRSVILRIPLSSAAPVGESFHFSIFHELKSTLALSNTTETVSHRKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPPYT TVNGS+LGNRIL RG QAEAE ST + TALKWVIRCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSLLGNRILKRGDQAEAEISTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS I NSECNLEA DQLKRVAAKDILN+GDQIFLPISVQVFP EKAR YCNKEEE
Sbjct: 121 KVRRGSSMIGNSECNLEARDQLKRVAAKDILNIGDQIFLPISVQVFPTEKARDYCNKEEE 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
           KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCL YDY EPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTS+LHSVFREKTFEAS HAN VDSEKK L+KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILQKRYWALVIGSPRRSKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPLGK V+DDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCP+TGRKHQL
Sbjct: 301 ITAPLGKFVMDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPVTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAHRN K FHSNFHKN+GKKQS PFGLDFESGSIAENNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSFPFGLDFESGSIAENNPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIIN 524
           LHLHCKQL+LPDVSFALHNDS+   CSRLECLELDAPLP YMQRSWDIIN
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSIDQKCSRLECLELDAPLPLYMQRSWDIIN 470

BLAST of ClCG02G006340 vs. NCBI nr
Match: XP_011658889.1 (RNA pseudouridine synthase 4, mitochondrial [Cucumis sativus] >KGN43951.2 hypothetical protein Csa_017092 [Cucumis sativus])

HSP 1 Score: 837.0 bits (2161), Expect = 8.7e-239
Identity = 414/471 (87.90%), Postives = 429/471 (91.08%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           M LRAL R SVILRTSPS   P G   +FSIFH LKSTLALSN +E VS+QKNEKGSNRW
Sbjct: 1   MGLRALFRPSVILRTSPSCTDPSGEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPPYT TVNGS+LGNRI+N G QAE  AST + TALKWVIRCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSILGNRIMNGGGQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSE NLEA DQLKRVAAKDILNVGDQIFLP+SVQVF  EK R YCNKEEE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
            F+HSLVL+KDPAILVINKPPGLPVQGGIGVKRSLDELAASCL Y Y EPPRLVHRLDRD
Sbjct: 181 TFMHSLVLHKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTS+LHSVFREKTFEAS HAN VDSEKK L KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPLGKIVLDDGRSEQIMVIDNAE MPFQQAIT+YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSEQIMVIDNAEPMPFQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAH+N K F SNFHKNQGKKQ LPFGLDFESGSIAENNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFCSNFHKNQGKKQFLPFGLDFESGSIAENNPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCKQL+LPDVSFALHNDSL  DCSRLECLELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDRDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of ClCG02G006340 vs. NCBI nr
Match: XP_008454996.1 (PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA0031338.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa] >TYK06791.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 833.6 bits (2152), Expect = 9.7e-238
Identity = 411/471 (87.26%), Postives = 425/471 (90.23%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL  L R SVILRTSPS   P     +FSIFH LKSTLALSN +E VS+QKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPPYT TVN S+LGNRILNRGAQAE E ST + TALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSE NLEA DQLKRVAAKDILNVGDQIFLPIS QVF  EK R YCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
            F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCL Y Y EPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTS+LHSVFREKTF+AS HAN VDSEKK L KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPLGKIVLDDGRSE+IMVIDNAE MPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAH+N K FH NFHKNQGKKQ LPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCKQL+LPDVSFALHNDSL  DCSRLECLELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of ClCG02G006340 vs. NCBI nr
Match: XP_023553811.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828.2 bits (2138), Expect = 4.1e-236
Identity = 408/471 (86.62%), Postives = 425/471 (90.23%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL A+LRRSVI R+ PSSAAPGG S HFSIF E KST ALSN TE VSEQKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPP+T TVNGS LGNRI+N GAQAEAE ST + TALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSECNLEA DQLKRVAA DILN+GD++ LPISVQVFP E+A   CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
           KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCL YDY EPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTSVLHS+FREKT  AS HAN VD EKK L+KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKKILQKRYWALVIGSPRRTKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPL K+VLDDGRSEQIMV+DNAESMPFQ AIT YKVIK+HHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVVDNAESMPFQHAITTYKVIKTHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAHRN K F  N HKNQGKKQSLPFGLDFESGSIAENNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCK L+LPDVS ALHNDSL HDCSRLE LELDAPLPSYMQRSWDIINN
Sbjct: 421 LHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

BLAST of ClCG02G006340 vs. NCBI nr
Match: KAG7011636.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 825.5 bits (2131), Expect = 2.6e-235
Identity = 407/471 (86.41%), Postives = 425/471 (90.23%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MV  A+LRRSVI R+ PSSAAPGG S HFSIF E KST ALS+ TE VSEQKNEKGSNRW
Sbjct: 1   MVFPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPP+T T NGS LGNRI+N GAQAEAE ST + TALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSECNLEA DQLKRVAA DILN+GD++ LPISVQVFP E+A   CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
           KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCL YDY EPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTSVLHS+FREKT  AS HAN VDSEKK L+KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPL K+VLDDGRSEQIMVIDNAESMPFQ AIT YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAHRN K F  N HKNQGKKQSLPFGLDFESGSIAEN+PR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCKQL+LPDVS ALHNDSL HDCSRLE LELDAPLPSYMQRSWDIINN
Sbjct: 421 LHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

BLAST of ClCG02G006340 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 8.5e-128
Identity = 235/441 (53.29%), Postives = 309/441 (70.07%), Query Frame = 0

Query: 93  ALSNTTENVSEQKNEKGSNRWFTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALK 152
           A   +T + S  + +    +W TLPP++ T++G+ +G  +L+ G   ++       TAL+
Sbjct: 35  AAHQSTRSYSTTQTDDSRGKWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALR 94

Query: 153 WVIRCCPELPRSLVQKLFRLRKVRRG-SSTIENSECNLEAGDQLKRVAAKDILNVGDQIF 212
           W++RC P+LPR+LVQKLFRLR+VRR  S +++  E       QLKRVAAK+ LNVGD+I+
Sbjct: 95  WILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIY 154

Query: 213 LPISV-----QVFPREKARGYCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKR 272
           LP+SV     Q  P +K    C+ EE KF+ SLVLYKDPAI+V+NKP GL VQGG G+K 
Sbjct: 155 LPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKT 214

Query: 273 SLDELAASCLRYDYLEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASTHAN 332
           S+DELAASCL++D  E PRLVHRLDRD SG+L++ RT T+ +VLHS+FREKT  AS +  
Sbjct: 215 SIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYG- 274

Query: 333 RVDSEKKFLEKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAI 392
            V    K L+++Y ALVIG P R +G I+APL K+V+DDG+SE+I V DN E +  Q AI
Sbjct: 275 -VKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAI 334

Query: 393 TRYKVIKSH-HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSN 452
           T Y+VI+S  HGYTWLEL PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAH+  + F S+
Sbjct: 335 TEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS 394

Query: 453 FHKNQGKKQSLPFGLDFESGSIAENNPRLHLHCKQLSLPDVSFALH--NDSLGHDCSRLE 512
              N  K+ S PFGLD + G ++   P LHLH KQ+ LP++S  L     S   D S L+
Sbjct: 395 -ENNPTKQSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLD 454

Query: 513 CLELDAPLPSYMQRSWDIINN 525
            L+ DAPLPS+MQ S++++ +
Sbjct: 455 SLKFDAPLPSHMQLSFNLLKS 469

BLAST of ClCG02G006340 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.7e-75
Identity = 164/428 (38.32%), Postives = 252/428 (58.88%), Query Frame = 0

Query: 115 TLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLRK 174
           +LPP+    +G  L  R           A+    TA+ WV     ++P+ +VQ  F  R 
Sbjct: 55  SLPPFPGGGDGDPLPGR---------KAAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRL 114

Query: 175 VRRGSSTIENS-ECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREK-------ARG 234
           V    S  E S E        LK++   D++  G +I LP+SV     +K       A  
Sbjct: 115 VYSECSDHEVSVETIKSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATL 174

Query: 235 YCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRL 294
           + NK+E +++  LV++KD AILV+NKPP +P++G + V  S+D LAA+ L Y   E P+L
Sbjct: 175 HPNKDEIEYLRRLVIHKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKL 234

Query: 295 VHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGS 354
           VHRLDR+SSG+L+ GRT  S + LH +F      A T++   ++  +   +RYWALVIG+
Sbjct: 235 VHRLDRESSGLLLFGRTKESFTRLHWLFTSVNL-AKTNSQVWNAACEAYMQRYWALVIGT 294

Query: 355 PRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVI-KSHHGYTWLELCP 414
           P+  +G+I+APL K++LDDG++E++++   +     Q+A+T Y+V+  + HG +W+EL P
Sbjct: 295 PKEREGIISAPLSKVLLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRP 354

Query: 415 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNLKAF-HSNFHKNQGKKQSL--PFGLDF 474
           LTGRKHQLRVHCAE LGTPIVGDYKYGW  H+  K     +F    G+   L  P GL+ 
Sbjct: 355 LTGRKHQLRVHCAEALGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEI 414

Query: 475 ESGSIAENNPRLHLHCKQLSLPDVSFALHNDSLGHD------CSRLECLELDAPLPSYMQ 525
           + GS+    P LHLHC+++ +P+++  L ++   H+        +   L   AP+P++M+
Sbjct: 415 QKGSVLSKVPLLHLHCREMVIPNIAKFLSSNGEWHENGAPWSKEKPNLLRFIAPMPAHMK 472

BLAST of ClCG02G006340 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.9e-74
Identity = 161/362 (44.48%), Postives = 222/362 (61.33%), Query Frame = 0

Query: 104 QKNEKGSNRWFTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPR 163
           +  E+G + W  LPP+      +    R ++RG   E        TA+KWV RCCP+LP 
Sbjct: 43  ETGERGKSPWLQLPPFAPL--DAAAAARAISRGG-GEGGDGEQGATAIKWVRRCCPDLPT 102

Query: 164 SLVQKLFRLRKVRRGSSTIENSECNLEAGD-QLKRVAAKDILNVGDQIFLPISV-QVFPR 223
           SLVQKLFRLRKV++   T E S  +  A   +L+RV+AKD L  GD +FLP+++ +    
Sbjct: 103 SLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVA 162

Query: 224 EKARGYCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYL 283
           EK + + N+ E  F+  L +YKD AI+V+NKPPG+PVQGG+G+K S+D L AS    +  
Sbjct: 163 EKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSS 222

Query: 284 EPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWA 343
           E PRLVHRLDRD SGIL++GR   STS+LH++FREKT +A       D  +  L+++Y A
Sbjct: 223 EAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTADA-----LADGTQHVLQRKYVA 282

Query: 344 LVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKS-HHGYTW 403
           LVIG+PR  KGL++APL KI+L DG+SE++ +  ++ +   Q A+T Y+VI+S   GYTW
Sbjct: 283 LVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTW 342

Query: 404 LELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRN--LKAFHSNFHKNQGKKQSLPF 461
           LEL P TGRKHQ        L   ++G      +  R+  L   H    +N   K S P 
Sbjct: 343 LELFPRTGRKHQ------RFLEPQLLGITSMDGKRIRSGCLFPCHGQLMRNCSGKGSFPL 389

BLAST of ClCG02G006340 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 7.1e-74
Identity = 165/455 (36.26%), Postives = 253/455 (55.60%), Query Frame = 0

Query: 90  STLALSNTTENVSEQKNEKGSNRWFTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNT 149
           S + ++N   ++   K      +  +LPP+     G  L  +  N GA  +   S    T
Sbjct: 29  SVIRVTNNVAHLGPPKQGPLPRQLISLPPFP----GHPLPGK--NAGADGDDGDSGGHVT 88

Query: 150 ALKWVIRCCPELPRSLVQKLFRLRKVRRGSSTIENSECNLEAGD-QLKRVAAKDILNVGD 209
           A+ WV     E+    +Q  F    V+       ++    E G   ++++   +++ +GD
Sbjct: 89  AISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGAIPMRKIKHNEVMQIGD 148

Query: 210 QIFLPISVQVFPREKARG-------YCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGG 269
           +I+LP+S+      K          Y N +E  ++  LV +KD AI+V+NKPP LPV+G 
Sbjct: 149 KIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDSAIIVLNKPPKLPVKGN 208

Query: 270 IGVKRSLDELAASCLRYDYLEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFRE-KTFE 329
           + +  S+D LAA+ L +   E PRLVHRLDR++SG+L+MGRT  S   LHSVF + K   
Sbjct: 209 VPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKESIDYLHSVFSDYKGRN 268

Query: 330 ASTHANRVDSEKKFLEKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESM 389
           +S  A     E  +  ++YWALVIGSP+  +GLI+APL K++LDDG+++++++   +   
Sbjct: 269 SSCKAWNKACEAMY--QQYWALVIGSPKEKEGLISAPLSKVLLDDGKTDRVVLAQGSGFE 328

Query: 390 PFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNL 449
             Q AIT YKV+    +G +W+EL P+T RKHQLRVHCAE LGTPIVGDYKYGW  H+  
Sbjct: 329 ASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPIVGDYKYGWFVHKRW 388

Query: 450 KAF-HSNFHKNQGKKQSL--PFGLDFESGSIAENNPRLHLHCKQLSLPDVSFALHNDSLG 509
           K     +     GK   L  P GLD + GS+    P LHLHC+++ LP+++  LH  +  
Sbjct: 389 KQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMVLPNIAKFLHVMNQQ 448

Query: 510 HD-------CSRLECLELDAPLPSYMQRSWDIINN 525
                      + + L   A +PS+M+ SW+++++
Sbjct: 449 ETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

BLAST of ClCG02G006340 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.6e-28
Identity = 91/285 (31.93%), Postives = 149/285 (52.28%), Query Frame = 0

Query: 152 KWVIRCCPELPRSLVQKLFRLRKVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIF 211
           K++ R  P L + +++K  R ++V   +   + +E NL       RV   D + + D   
Sbjct: 15  KYLKRLYPSLTQGVIEKALRQKQV---TVNFQKAEANL-------RVKEGDTIFINDYFN 74

Query: 212 LPIS---VQVFPREKARGYCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSL 271
           LP++     VF   +      K  +K +   ++Y+D  ++ INKP  L  QGG  +  S+
Sbjct: 75  LPVTQHETLVFADAEI-----KLAKKILTDYLIYEDDHLIAINKPASLATQGGSKINLSI 134

Query: 272 DELAASCLRYDYLEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASTHANRV 331
           D  A   L Y   +  +LVHRLD+++SG+L++ +   S+  LH  F+E            
Sbjct: 135 DS-ALKYLNYQGAD-FKLVHRLDKETSGLLLIAKNYLSSVKLHDAFKE------------ 194

Query: 332 DSEKKFLEKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITR 391
               K + K+Y+A+  G P ++ G++ + +GK     GR  +I  ID+      + AIT 
Sbjct: 195 ----KLVIKKYFAITYGRPVKNVGIVKSNIGK---SKGRMFKITDIDSDNG---KLAITY 254

Query: 392 YKVIKSHHGYTWL-ELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 433
           YK++KS +   +L E  P+TGR HQLR+H A++LG PI+GD KYG
Sbjct: 255 YKLLKSLNNNLFLIEFMPVTGRMHQLRLH-AQLLGCPILGDDKYG 259

BLAST of ClCG02G006340 vs. ExPASy TrEMBL
Match: A0A5D3C4F5 (RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00930 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.7e-238
Identity = 411/471 (87.26%), Postives = 425/471 (90.23%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL  L R SVILRTSPS   P     +FSIFH LKSTLALSN +E VS+QKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPPYT TVN S+LGNRILNRGAQAE E ST + TALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSE NLEA DQLKRVAAKDILNVGDQIFLPIS QVF  EK R YCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
            F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCL Y Y EPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTS+LHSVFREKTF+AS HAN VDSEKK L KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPLGKIVLDDGRSE+IMVIDNAE MPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAH+N K FH NFHKNQGKKQ LPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCKQL+LPDVSFALHNDSL  DCSRLECLELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of ClCG02G006340 vs. ExPASy TrEMBL
Match: A0A1S3BZE7 (RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495275 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.7e-238
Identity = 411/471 (87.26%), Postives = 425/471 (90.23%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL  L R SVILRTSPS   P     +FSIFH LKSTLALSN +E VS+QKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPPYT TVN S+LGNRILNRGAQAE E ST + TALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSE NLEA DQLKRVAAKDILNVGDQIFLPIS QVF  EK R YCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
            F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCL Y Y EPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTS+LHSVFREKTF+AS HAN VDSEKK L KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPLGKIVLDDGRSE+IMVIDNAE MPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAH+N K FH NFHKNQGKKQ LPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCKQL+LPDVSFALHNDSL  DCSRLECLELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of ClCG02G006340 vs. ExPASy TrEMBL
Match: A0A6J1GMP9 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455375 PE=4 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.7e-235
Identity = 408/471 (86.62%), Postives = 425/471 (90.23%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL A+LRRSVI R+ PSSAAPGG S HFSIF E KST ALS  TE VSEQKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPP+T T NGS LGNRI+N GAQAEAE ST + TALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSECNLEA DQLKRVAA DILN+GD++ LPISVQVFP E+A   CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
           KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCL YDY EPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTSVLHS+FREKT  AS HAN VDSEKK L+KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 413
           ITAPL K+VLDDGRSEQIMVIDNAESMPFQ AIT YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 414 RVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNPR 473
           RVHCAEVLGTPIVGDYKYGWQAHRN K F  N HKNQGKKQSLPFGLDFESGSIAEN+PR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSPR 420

Query: 474 LHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           LHLHCKQL+LPDVS ALHNDSL HDCSRLE LELDAPLPSYMQRSWDIINN
Sbjct: 421 LHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

BLAST of ClCG02G006340 vs. ExPASy TrEMBL
Match: A0A6J1IBC5 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470950 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.7e-233
Identity = 405/472 (85.81%), Postives = 422/472 (89.41%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNEKGSNRW 113
           MVL ALLRRSVI R+ PSSAAPGG S HFSIF E KST ALSN TE VSEQKNEKGSNRW
Sbjct: 1   MVLPALLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 114 FTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRLR 173
           FTLPP+T TVNGS LGNRI+N GAQ+EAE ST + TALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQSEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 174 KVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEE 233
           KVRRGSS IENSECNLEA DQLKRVAA DILN+GD++ LPISVQVFP E+A   CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 234 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDRD 293
           KFIHSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCL YDY EPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 294 SSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKGL 353
           SSGILIMGRTSTSTSVLHS+FREKT  AS HAN VD EK  L+KRYWALVIGSP  +KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKMILQKRYWALVIGSPSHTKGL 300

Query: 354 ITAPLGKIVLDDGRSEQIMVIDNAES-MPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 413
           ITAPL K+VLDDGRSEQIMV+DNAES MPFQ AIT YKVIKSHHGYTW+ELCPLTGRKHQ
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVVDNAESIMPFQHAITTYKVIKSHHGYTWVELCPLTGRKHQ 360

Query: 414 LRVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNP 473
           LRVHCAE LGTPIVGDYKYGWQAHRNLK F  N HKNQGKKQSLPFGLDFESGSIAENNP
Sbjct: 361 LRVHCAEALGTPIVGDYKYGWQAHRNLKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNP 420

Query: 474 RLHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           RLHLHCK L+LPDVS ALHNDSL HDCSRLE LELDAPLPSYMQRSWDIINN
Sbjct: 421 RLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 472

BLAST of ClCG02G006340 vs. ExPASy TrEMBL
Match: A0A6J1D432 (RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017405 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.9e-233
Identity = 400/472 (84.75%), Postives = 432/472 (91.53%), Query Frame = 0

Query: 54  MVLRALLRRSVILRTSPSSAAPGGGSFHFSIFHELKSTLALSNTTENVSEQKNE-KGSNR 113
           M+L ALLRRS+IL  +PSS A GG S HFS+FHELKST ALSNT+E +SE+KNE KG NR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 114 WFTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALKWVIRCCPELPRSLVQKLFRL 173
           WFTLPPYT TV+GSVLGNRILN+G+QAE E ST + TALKWV+RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 174 RKVRRGSSTIENSECNLEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEE 233
           RK+RRGSS IE+SECNLEA +QLKRVAAKDILNVGDQIFLPISVQVFPRE+AR YCNKEE
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 234 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLRYDYLEPPRLVHRLDR 293
           EKF+HSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCL YDY EPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 294 DSSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKKFLEKRYWALVIGSPRRSKG 353
           DSSGIL+MGRTSTSTSVLHS+FREKTF AS H   VDSEKK L+K+YWALVIGSPRR  G
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRRPNG 300

Query: 354 LITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 413
           LITAPLGKIVLDDGRSE+IMVIDNAESM FQQAIT Y+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360

Query: 414 LRVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSNFHKNQGKKQSLPFGLDFESGSIAENNP 473
           LRVHCAEVLGTPIVGDYKYGWQAHRN + F SNFHKNQGKKQSLPFGLDFE+GS+AEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSP 420

Query: 474 RLHLHCKQLSLPDVSFALHNDSLGHDCSRLECLELDAPLPSYMQRSWDIINN 525
           RLHLHCKQL+LP+VS AL NDSL HD S LECLELDAPLPSYM+RSWDIINN
Sbjct: 421 RLHLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

BLAST of ClCG02G006340 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 458.8 bits (1179), Expect = 6.0e-129
Identity = 235/441 (53.29%), Postives = 309/441 (70.07%), Query Frame = 0

Query: 93  ALSNTTENVSEQKNEKGSNRWFTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNTALK 152
           A   +T + S  + +    +W TLPP++ T++G+ +G  +L+ G   ++       TAL+
Sbjct: 35  AAHQSTRSYSTTQTDDSRGKWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALR 94

Query: 153 WVIRCCPELPRSLVQKLFRLRKVRRG-SSTIENSECNLEAGDQLKRVAAKDILNVGDQIF 212
           W++RC P+LPR+LVQKLFRLR+VRR  S +++  E       QLKRVAAK+ LNVGD+I+
Sbjct: 95  WILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIY 154

Query: 213 LPISV-----QVFPREKARGYCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKR 272
           LP+SV     Q  P +K    C+ EE KF+ SLVLYKDPAI+V+NKP GL VQGG G+K 
Sbjct: 155 LPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKT 214

Query: 273 SLDELAASCLRYDYLEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASTHAN 332
           S+DELAASCL++D  E PRLVHRLDRD SG+L++ RT T+ +VLHS+FREKT  AS +  
Sbjct: 215 SIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYG- 274

Query: 333 RVDSEKKFLEKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAI 392
            V    K L+++Y ALVIG P R +G I+APL K+V+DDG+SE+I V DN E +  Q AI
Sbjct: 275 -VKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAI 334

Query: 393 TRYKVIKSH-HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNLKAFHSN 452
           T Y+VI+S  HGYTWLEL PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAH+  + F S+
Sbjct: 335 TEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS 394

Query: 453 FHKNQGKKQSLPFGLDFESGSIAENNPRLHLHCKQLSLPDVSFALH--NDSLGHDCSRLE 512
              N  K+ S PFGLD + G ++   P LHLH KQ+ LP++S  L     S   D S L+
Sbjct: 395 -ENNPTKQSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLD 454

Query: 513 CLELDAPLPSYMQRSWDIINN 525
            L+ DAPLPS+MQ S++++ +
Sbjct: 455 SLKFDAPLPSHMQLSFNLLKS 469

BLAST of ClCG02G006340 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 279.6 bits (714), Expect = 5.0e-75
Identity = 165/455 (36.26%), Postives = 253/455 (55.60%), Query Frame = 0

Query: 90  STLALSNTTENVSEQKNEKGSNRWFTLPPYTTTVNGSVLGNRILNRGAQAEAEASTVSNT 149
           S + ++N   ++   K      +  +LPP+     G  L  +  N GA  +   S    T
Sbjct: 29  SVIRVTNNVAHLGPPKQGPLPRQLISLPPFP----GHPLPGK--NAGADGDDGDSGGHVT 88

Query: 150 ALKWVIRCCPELPRSLVQKLFRLRKVRRGSSTIENSECNLEAGD-QLKRVAAKDILNVGD 209
           A+ WV     E+    +Q  F    V+       ++    E G   ++++   +++ +GD
Sbjct: 89  AISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGAIPMRKIKHNEVMQIGD 148

Query: 210 QIFLPISVQVFPREKARG-------YCNKEEEKFIHSLVLYKDPAILVINKPPGLPVQGG 269
           +I+LP+S+      K          Y N +E  ++  LV +KD AI+V+NKPP LPV+G 
Sbjct: 149 KIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDSAIIVLNKPPKLPVKGN 208

Query: 270 IGVKRSLDELAASCLRYDYLEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFRE-KTFE 329
           + +  S+D LAA+ L +   E PRLVHRLDR++SG+L+MGRT  S   LHSVF + K   
Sbjct: 209 VPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKESIDYLHSVFSDYKGRN 268

Query: 330 ASTHANRVDSEKKFLEKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESM 389
           +S  A     E  +  ++YWALVIGSP+  +GLI+APL K++LDDG+++++++   +   
Sbjct: 269 SSCKAWNKACEAMY--QQYWALVIGSPKEKEGLISAPLSKVLLDDGKTDRVVLAQGSGFE 328

Query: 390 PFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNL 449
             Q AIT YKV+    +G +W+EL P+T RKHQLRVHCAE LGTPIVGDYKYGW  H+  
Sbjct: 329 ASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPIVGDYKYGWFVHKRW 388

Query: 450 KAF-HSNFHKNQGKKQSL--PFGLDFESGSIAENNPRLHLHCKQLSLPDVSFALHNDSLG 509
           K     +     GK   L  P GLD + GS+    P LHLHC+++ LP+++  LH  +  
Sbjct: 389 KQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMVLPNIAKFLHVMNQQ 448

Query: 510 HD-------CSRLECLELDAPLPSYMQRSWDIINN 525
                      + + L   A +PS+M+ SW+++++
Sbjct: 449 ETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

BLAST of ClCG02G006340 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 2.7e-12
Identity = 79/341 (23.17%), Postives = 131/341 (38.42%), Query Frame = 0

Query: 131 RILNRGAQAE--AEASTVSNTALKWVIRCCPELPRSLVQKLFRLRKVRRGSSTIENSECN 190
           R+ N G + E   +A+        W+      + R+ VQ   RL  V      ++    N
Sbjct: 63  RVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 122

Query: 191 LEAGDQLKRVAAKDILNVGDQIFLPISVQVFPREKARGYCNKEEEKFIHSLVLYKDPAIL 250
           +++GD++    ++           P+               K E + I   ++Y+D  +L
Sbjct: 123 VKSGDEVNCTISE---------LQPL---------------KAEAEDIPLDIVYEDKHVL 182

Query: 251 VINKP--------PGLPVQGGIGVKRSLDELAASCLRYDYLEP----------------- 310
           V+NKP        PG P   G  V   L   +  C+ Y   E                  
Sbjct: 183 VVNKPAHMVVHPAPGNPT--GTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTT 242

Query: 311 ----------PRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASTHANRVDSEKK 370
                     P +VHRLD+ ++G+L++ +   S + L   F+  T               
Sbjct: 243 SPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHT--------------- 302

Query: 371 FLEKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIK 430
            +E+ Y +L  G P   +G I  P+G+      R     +         + A +RYKVI+
Sbjct: 303 -IERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAASRYKVIE 358

Query: 431 SH--HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 433
           +    G   +E    TGR HQ+R H A+ +G P++GD  YG
Sbjct: 363 TFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYG 358

BLAST of ClCG02G006340 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 66.2 bits (160), Expect = 8.7e-11
Identity = 58/201 (28.86%), Postives = 95/201 (47.26%), Query Frame = 0

Query: 240 VLYKDPAILVINKPPGLPV-QGGIGVKRSLDELAASCL--RYDYL-----EPPRLVHRLD 299
           VLY+D  ++ +NKP GL V  GG+  +R++      C      Y+       P  VHRL 
Sbjct: 99  VLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLG 158

Query: 300 RDSSGILIMGRTSTSTSVLHSVFREKT-FEASTHANRVDSEKKFLEKRYWALVIGSPRRS 359
           R +SGIL+  +T  + + L + F E T    S + ++     + L K Y AL  G     
Sbjct: 159 RGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEED 218

Query: 360 KGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRK 419
           + +I  P+G +V   G ++ + V        F +     +  + +     +E+   +GR 
Sbjct: 219 EVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEI--QSGRP 278

Query: 420 HQLRVHCAEVLGTPIVGDYKY 432
           HQ+R+H A  +G P+VGD  Y
Sbjct: 279 HQIRIHLA-YMGHPLVGDPLY 295

BLAST of ClCG02G006340 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 3.7e-09
Identity = 55/196 (28.06%), Postives = 92/196 (46.94%), Query Frame = 0

Query: 240 VLYKDPAILVINKPPGLPV-QGGIGVKRSLDELAASCL--RYDYL-----EPPRLVHRLD 299
           VLY+D  ++ +NKP GL V  GG+  +R++      C      Y+       P  VHRL 
Sbjct: 129 VLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLG 188

Query: 300 RDSSGILIMGRTSTSTSVLHSVFREKT-FEASTHANRVDSEKKFLEKRYWALVIGSPRRS 359
           R +SGIL+  +T  + + L + F E T    S + ++     + L K Y AL  G     
Sbjct: 189 RGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEED 248

Query: 360 KGLITAPLGKIVLDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRK 419
           + +I  P+G +V   G ++ + V        F +     +  + +     +E+   +GR 
Sbjct: 249 EVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEI--QSGRP 308

Query: 420 HQLRVHCAEVLGTPIV 427
           HQ+R+H A  +G P+V
Sbjct: 309 HQIRIHLA-YMGHPLV 320