Chy5G104450 (gene) Cucumber (hystrix) v1

Overview


Name	Chy5G104450
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	cytochrome B5-like protein
Location	chrH05: 18045184 .. 18046741 (+)
RNA-Seq Expression	Chy5G104450
Synteny	Chy5G104450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGTAAGTTTGAAGAAGTATATTTGTTTCATTTCTTCAGTTCTCCAATGCATAGATTCACATCGATTTGAACAGACAATGCTATTTTCTAATGCAGGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGGTACTTTGGATATGGAGGTTTTATTTTTAGTACATTTACTGGAAATTTGAAGACTTAGTTGTCTTTTAGATTCAATTTTATAAATGAATTGATCAATTCTTTGCCAGCTGAGAAGCTGGGACGTGAATGTTTTTAGCTTCAGTAGCATAGGTAAATATTTTGGCTTGTGCAATCTTCTATTTCCAGAATCCAGCTAAAGATAATGTATTGCTTTACTATTGAATCCTGTTAAAAATATGTTGGAGTTTTCAAATGTATAATGTCCAATCTATTCAACCTTTTCTCAAAGATGAATAAGTGGGAAAAAAAGTTGTTGAGTAAATTGATTGATTTTCTAGTCCAAATGTTTTAAATGATTGTGCTGTTGTACTAACCAACTGCTTATAACTAGGAGTGCTAATGCTTTGTGGCTTTGAGTCTTTGACTATGGATATTGACTAGGATTATAAATACTGAACTTGTACAATGCACAGTTGAGAAACAGAAAAGAATCTGTAGTTTACAAGAGATTTAGTGAAACAGTTTGTGCCTTCATTCACCATAGAAACAAAGTTGTACGTCCAAATTTAATTCTCAAATGTTTGAAGCGCGTTTTAAGTTATTAACTCTCTGTGGGATTCCTTTCCGTTATTGCTTGGATTGCTGAACTTTAGTATTGTGATTTCAAATCTCTTGTTTCTTGCTATTAGTCATCTAAAGTCTACTACAGTAGAGATGAAGTCTCTGTTCATAACGAGAGAACTGATTGTTGGGTCATTATCAAGAACAAGGTATAATATTTCTATCTTCTGGTTTGATATCCAAGCTCTTAGCAAAAGTTAGAGTTTGGTCGTATATTATAGTTGTACTTATAACCTTTTTTCGATTATTCTTGAGCATAGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGTAAACCAGGCTGACATCACCCATCTGTTATTGTCATGTTAAATTGATATGTCCAAACAATCTTGATTGATTGAATGCTCTAGAATTGTTTTGGCATAAATAGACAATAATTAAGATCACCATGCTTGATTCACTAAACAAAGCCCATCGAGGGAGAGAATATTGCCTATTAAGTTTCACTCGCAACTAGACAGCATTCACTAAGAAAGACACCGAACCTTTTCCCTTCATTTGAACTTCAAACTATAATATTAGATTCCATCATTGCAACAATCTTCTAAACATGTTAATTTCGACCTCGATACTTATTATGCAGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAA

mRNA sequence

ATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGTCATCTAAAGTCTACTACAGTAGAGATGAAGTCTCTGTTCATAACGAGAGAACTGATTGTTGGGTCATTATCAAGAACAAGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAA

Coding sequence (CDS)

ATGGTGATCGTGATAGCTGCATTGCTTCTCAGTGTTTTGTTGGGAGCTCTATTTTTAATCCCACGCGACCGAAATGCACAAAAGGTCCAACTAAATCCTATCAACAAGTCATCTAAAGTCTACTACAGTAGAGATGAAGTCTCTGTTCATAACGAGAGAACTGATTGTTGGGTCATTATCAAGAACAAGGTGTATGATGTTACCTCCTATGTTGAAGAACATCCTGGAGGGGATGCCATCCTAACACATGCTGGGGATGATTCTACAGAAGGGTTTTATGGGCCACAACACGCAACTCGAGTCTTTGACATGATTGAAGACTTCTATATTGGAGATCTGAAGCTGTAA

Protein sequence

MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL*

Homology

[Close]

BLAST of Chy5G104450 vs. ExPASy Swiss-Prot
Match: O22704 (Cytochrome B5-like protein OS=Arabidopsis thaliana OX=3702 GN=CB5LP PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 7.8e-34
Identity = 73/120 (60.83%), Postives = 86/120 (71.67%), Query Frame = 0


Query: 2   VIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVY--------YSRDEVSVHNER 61
           +I +  LLL  L+ ALFLI   R            SS+          YS+ EV+VHN+R
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWVIIKNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 114
            DCW+IIK+KVYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMIEDFYIG+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of Chy5G104450 vs. ExPASy Swiss-Prot
Match: Q9ZNW2 (Acyl-lipid (9-3)-desaturase OS=Physcomitrium patens OX=3218 GN=DES6 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 9.6e-16
Identity = 34/69 (49.28%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 46  EVSVHNERTDCWVIIKNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMI 105
           EV+VHN+ +DCW+++KNKVYDV+++ +EHPGG  I T+ G D T+ F    HA   + ++
Sbjct: 109 EVAVHNKPSDCWIVVKNKVYDVSNFADEHPGGSVISTYFGRDGTDVF-SSFHAASTWKIL 168

Query: 106 EDFYIGDLK 115
           +DFYIGD++
Sbjct: 169 QDFYIGDVE 176

[Close]

BLAST of Chy5G104450 vs. ExPASy Swiss-Prot
Match: Q9FDW8 (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.1e-14
Identity = 34/73 (46.58%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 42  YSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           YS +E + HN++ DCWV+I  KVYDV+SY++EHPGG D +L  AG D+T+ F    H+  
Sbjct: 8   YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67

Query: 102 VFDMIEDFYIGDL 114
             +++E ++IG+L
Sbjct: 68  ARELMEKYFIGEL 80

[Close]

BLAST of Chy5G104450 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 3.1e-14
Identity = 32/81 (39.51%), Postives = 56/81 (69.14%), Query Frame = 0


Query: 34  INKSSKVYYSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGG-DAILTHAGDDSTEGF 93
           ++  +K  Y+ +EV+ HN + DCW+II  KVY+V+ ++E+HPGG D +L+  G D+T+ F
Sbjct: 1   MSNDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDF 60

Query: 94  YGPQHATRVFDMIEDFYIGDL 114
               H+T    M++++Y+GD+
Sbjct: 61  EDVGHSTTARAMMDEYYVGDI 81

[Close]

BLAST of Chy5G104450 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 6.9e-14
Identity = 35/78 (44.87%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 37  SSKVYYSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGP 96
           S K     +EVS HN+  DCW+II  KVYDVT ++++HPGGD  +L+  G D+T  F   
Sbjct: 3   SEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 97  QHATRVFDMIEDFYIGDL 114
            H+    DM+E +YIG++
Sbjct: 63  GHSDTARDMMEKYYIGEI 80

[Close]

BLAST of Chy5G104450 vs. ExPASy TrEMBL
Match: A0A1S3C893 (cytochrome B5-like protein OS=Cucumis melo OX=3656 GN=LOC103498106 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-56
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYS+DEVSVHN+RTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of Chy5G104450 vs. ExPASy TrEMBL
Match: A0A0A0LFU2 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000900 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.4e-56
Identity = 112/115 (97.39%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYS+DEVSVHN+RTDCWVII
Sbjct: 121 MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 180

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KN+VYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 181 KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 235

[Close]

BLAST of Chy5G104450 vs. ExPASy TrEMBL
Match: A0A6J1FBC0 (cytochrome B5-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443883 PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.6e-48
Identity = 97/115 (84.35%), Postives = 108/115 (93.91%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKV YS+DEVSVHN+RTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNTNKASKV-YSKDEVSVHNKRTDCWIII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Chy5G104450 vs. ExPASy TrEMBL
Match: A0A6J1HN83 (cytochrome B5-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465731 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.8e-48
Identity = 96/115 (83.48%), Postives = 107/115 (93.04%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKV Y +DEVSVHN+RTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNSTNKASKV-YGKDEVSVHNKRTDCWIII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           KNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Chy5G104450 vs. ExPASy TrEMBL
Match: A0A6J1FAG5 (cytochrome B5-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443883 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.1e-46
Identity = 97/118 (82.20%), Postives = 108/118 (91.53%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDR---NAQKVQLNPINKSSKVYYSRDEVSVHNERTDCW 60
           M+I++AAL+L VL GAL LIPRDR   NAQKVQLN  NK+SKV YS+DEVSVHN+RTDCW
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRKFGNAQKVQLNNTNKASKV-YSKDEVSVHNKRTDCW 60

Query: 61  VIIKNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 116
           +IIKNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  IIIKNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 117

[Close]

BLAST of Chy5G104450 vs. NCBI nr
Match: XP_008458811.1 (PREDICTED: cytochrome B5-like protein [Cucumis melo])

HSP 1 Score: 228 bits (581), Expect = 3.29e-75
Identity = 113/115 (98.26%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYS+DEVSVHN+RTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115
           KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of Chy5G104450 vs. NCBI nr
Match: XP_004141019.1 (cytochrome B5-like protein [Cucumis sativus] >KAE8651507.1 hypothetical protein Csa_019513 [Cucumis sativus])

HSP 1 Score: 227 bits (578), Expect = 9.44e-75
Identity = 112/115 (97.39%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYS+DEVSVHN+RTDCWVII
Sbjct: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSKDEVSVHNKRTDCWVII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115
           KN+VYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL
Sbjct: 61  KNRVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115

[Close]

BLAST of Chy5G104450 vs. NCBI nr
Match: XP_038890467.1 (cytochrome B5-like protein [Benincasa hispida] >XP_038890468.1 cytochrome B5-like protein [Benincasa hispida] >XP_038890469.1 cytochrome B5-like protein [Benincasa hispida])

HSP 1 Score: 204 bits (518), Expect = 1.30e-65
Identity = 102/115 (88.70%), Postives = 110/115 (95.65%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           MVIV+AAL+LSVLLGAL LIPR+RN QKVQLN INK SKVY S+DEVSVHN+RTDCWVII
Sbjct: 1   MVIVVAALVLSVLLGALILIPRNRNVQKVQLNAINKPSKVY-SKDEVSVHNKRTDCWVII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115
           KNKVY+VTSYVEEHPGGDAILTHAGDDSTEGFYGPQHA+RVFDMIEDFYIGDL+L
Sbjct: 61  KNKVYNVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHASRVFDMIEDFYIGDLRL 114

[Close]

BLAST of Chy5G104450 vs. NCBI nr
Match: KAG7021304.1 (Cytochrome B5-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 202 bits (515), Expect = 3.72e-65
Identity = 98/115 (85.22%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN INK+SKVY S+DEVSVHN+RTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNINKASKVY-SKDEVSVHNKRTDCWIII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115
           KNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Chy5G104450 vs. NCBI nr
Match: XP_022937485.1 (cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_022937486.1 cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_022937487.1 cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_023536891.1 cytochrome B5-like protein [Cucurbita pepo subsp. pepo] >XP_023536892.1 cytochrome B5-like protein [Cucurbita pepo subsp. pepo] >XP_023536893.1 cytochrome B5-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 201 bits (510), Expect = 2.15e-64
Identity = 97/115 (84.35%), Postives = 108/115 (93.91%), Query Frame = 0


Query: 1   MVIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVYYSRDEVSVHNERTDCWVII 60
           M+I++AAL+L VL GAL LIPRDRNAQKVQLN  NK+SKVY S+DEVSVHN+RTDCW+II
Sbjct: 1   MMIIVAALVLGVLFGALILIPRDRNAQKVQLNNTNKASKVY-SKDEVSVHNKRTDCWIII 60

Query: 61  KNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDLKL 115
           KNKVYDVTSYVEEHPGGDAIL HAGDDSTEGFYGPQHATRVFDMI+DFY+GDL+L
Sbjct: 61  KNKVYDVTSYVEEHPGGDAILAHAGDDSTEGFYGPQHATRVFDMIDDFYVGDLRL 114

[Close]

BLAST of Chy5G104450 vs. TAIR 10
Match: AT1G60660.1 (cytochrome B5-like protein )

HSP 1 Score: 144.4 bits (363), Expect = 5.6e-35
Identity = 73/120 (60.83%), Postives = 86/120 (71.67%), Query Frame = 0


Query: 2   VIVIAALLLSVLLGALFLIPRDRNAQKVQLNPINKSSKVY--------YSRDEVSVHNER 61
           +I +  LLL  L+ ALFLI   R            SS+          YS+ EV+VHN+R
Sbjct: 1   MIAVIGLLLGFLVSALFLIQGKRRRTNDNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKR 60

Query: 62  TDCWVIIKNKVYDVTSYVEEHPGGDAILTHAGDDSTEGFYGPQHATRVFDMIEDFYIGDL 114
            DCW+IIK+KVYD+TSYVEEHPGGDAIL HAGDDST+GF+GPQHATRVFDMIEDFYIG+L
Sbjct: 61  NDCWIIIKDKVYDITSYVEEHPGGDAILDHAGDDSTDGFFGPQHATRVFDMIEDFYIGEL 120

[Close]

BLAST of Chy5G104450 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 80.9 bits (198), Expect = 7.6e-16
Identity = 34/73 (46.58%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 42  YSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           YS +E + HN++ DCWV+I  KVYDV+SY++EHPGG D +L  AG D+T+ F    H+  
Sbjct: 8   YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67

Query: 102 VFDMIEDFYIGDL 114
             +++E ++IG+L
Sbjct: 68  ARELMEKYFIGEL 80

[Close]

BLAST of Chy5G104450 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 77.4 bits (189), Expect = 8.4e-15
Identity = 31/73 (42.47%), Postives = 50/73 (68.49%), Query Frame = 0


Query: 42  YSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGPQHATR 101
           ++  EVS H+   DCW++I  KVYDVT ++++HPGGD  ILT  G D+T+ F    H++ 
Sbjct: 8   FTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSST 67

Query: 102 VFDMIEDFYIGDL 114
              M++++Y+GD+
Sbjct: 68  AKAMLDEYYVGDI 80

[Close]

BLAST of Chy5G104450 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 76.3 bits (186), Expect = 1.9e-14
Identity = 30/73 (41.10%), Postives = 50/73 (68.49%), Query Frame = 0


Query: 42  YSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGG-DAILTHAGDDSTEGFYGPQHATR 101
           ++  EVS HN+  DCW++I  KVY+VT ++E+HPGG D +L+  G D+T+ F    H+  
Sbjct: 8   FTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSES 67

Query: 102 VFDMIEDFYIGDL 114
             +M+E +Y+G++
Sbjct: 68  AREMMEQYYVGEI 80

[Close]

BLAST of Chy5G104450 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 75.9 bits (185), Expect = 2.4e-14
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 37  SSKVYYSRDEVSVHNERTDCWVIIKNKVYDVTSYVEEHPGGD-AILTHAGDDSTEGFYGP 96
           S +   S +EVS HN+  DCW+II  KVYDVT ++++HPGGD  +L+  G D+T  F   
Sbjct: 3   SDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDV 62

Query: 97  QHATRVFDMIEDFYIGDL 114
            H+    DM++ ++IG++
Sbjct: 63  GHSDTARDMMDKYFIGEI 80

The following BLAST results are available for this feature:

BLAST of Chy5G104450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22704	7.8e-34	60.83	Cytochrome B5-like protein OS=Arabidopsis thaliana OX=3702 GN=CB5LP PE=2 SV=1	[more]
Q9ZNW2	9.6e-16	49.28	Acyl-lipid (9-3)-desaturase OS=Physcomitrium patens OX=3218 GN=DES6 PE=1 SV=1	[more]
Q9FDW8	1.1e-14	46.58	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
P49100	3.1e-14	39.51	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
P40934	6.9e-14	44.87	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]

BLAST of Chy5G104450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C893	1.5e-56	98.26	cytochrome B5-like protein OS=Cucumis melo OX=3656 GN=LOC103498106 PE=3 SV=1	[more]
A0A0A0LFU2	3.4e-56	97.39	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1FBC0	2.6e-48	84.35	cytochrome B5-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A6J1HN83	5.8e-48	83.48	cytochrome B5-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146573...	[more]
A0A6J1FAG5	1.1e-46	82.20	cytochrome B5-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]

BLAST of Chy5G104450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458811.1	3.29e-75	98.26	PREDICTED: cytochrome B5-like protein [Cucumis melo]	[more]
XP_004141019.1	9.44e-75	97.39	cytochrome B5-like protein [Cucumis sativus] >KAE8651507.1 hypothetical protein ...	[more]
XP_038890467.1	1.30e-65	88.70	cytochrome B5-like protein [Benincasa hispida] >XP_038890468.1 cytochrome B5-lik...	[more]
KAG7021304.1	3.72e-65	85.22	Cytochrome B5-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022937485.1	2.15e-64	84.35	cytochrome B5-like protein isoform X2 [Cucurbita moschata] >XP_022937486.1 cytoc...	[more]

BLAST of Chy5G104450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60660.1	5.6e-35	60.83	cytochrome B5-like protein	[more]
AT1G26340.1	7.6e-16	46.58	cytochrome B5 isoform A	[more]
AT5G48810.1	8.4e-15	42.47	cytochrome B5 isoform D	[more]
AT2G32720.1	1.9e-14	41.10	cytochrome B5 isoform B	[more]
AT5G53560.1	2.4e-14	42.31	cytochrome B5 isoform E	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 101..113 score: 33.8 coord: 74..88 score: 32.12 coord: 64..74 score: 49.45
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 42..114 e-value: 1.9E-24 score: 97.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 44..113 e-value: 4.8E-21 score: 74.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 39..114 score: 24.990038
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 34..115 e-value: 1.2E-28 score: 100.9
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 38..114
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 2..114
None	No IPR available	PANTHER	PTHR19359:SF95	CYTOCHROME B5-LIKE PROTEIN	coord: 2..114
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 70..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy5G104450.1	Chy5G104450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding