Chy4G078830 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G078830
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectinesterase
Location	chrH04: 17860892 .. 17861752 (-)
RNA-Seq Expression	Chy4G078830
Synteny	Chy4G078830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTATATTTTAACAGGGAAAAAGTGACGATACCCTCAGACAAATCATGCATATTTCTAGACGGAAGTGGGCTTAAAGTGACTGAAGTTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTTCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATATACTTATAACTGTTTAATTTCCCTATCTTTCAAATAATGTATATATTTTCTTTTTGTTTTATGTCTATTATCTGTGATTCAAAATTGGCAGAATACTTATAATGCACGTGGAAGTGTGATGAGACGTGAAGATATAAAACCAGCACTTGCAGCTCTAATCCAAGGAAAAAAGTGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGTCAGTCTATCTATAAGGTTAACTTCTAAATTATAAGCTTACATGTGTGTTTGTATATATGTGTGAGAGCTTGGAAGTCAAATTGAAGTGTTGAGTTTTTCTTTTTTGTTTGTTTGTTTGTTGTGTTTAACGCAGGAATGTGTGATAAACGTACCCGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAGGGGCTTATAGACCTTTCTCCACCGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAAGTTGGGCATGAGTAA

mRNA sequence

ATGTTATATTTTAACAGGGAAAAAGTGACGATACCCTCAGACAAATCATGCATATTTCTAGACGGAAGTGGGCTTAAAGTGACTGAAGTTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTTCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGAATGTGTGATAAACGTACCCGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAGGGGCTTATAGACCTTTCTCCACCGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAAGTTGGGCATGAGTAA

Coding sequence (CDS)

ATGTTATATTTTAACAGGGAAAAAGTGACGATACCCTCAGACAAATCATGCATATTTCTAGACGGAAGTGGGCTTAAAGTGACTGAAGTTCATTGGAATGACCATGAAACAACAGCTGCTAGTCCCACCTTTACTTCTTCTGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACCCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTTGGAATGTGTGATAAACGTACCCGTCAATGCATATGCTCCATTACTAAATGAAGGTTTCATAACAGCTCAAGGAAAGGAGAATCCAAATGAAAGCAGTGGATTTGTATTCCTTAGATGCATAGTGCAGGGAAGTGGGAATGTTTTTCTTGGAGGGGCTTATAGACCTTTCTCCACCGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTAACCCTGCTGGTTGGAATTCTTGGCTACAAGTTGGGCATGAGTAA

Protein sequence

MLYFNREKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRILCGMVLAVIYSPSATLKVSLMSFLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFSTVIFHLCFLPSCINPAGWNSWLQVGHE*

Homology

[Close]

BLAST of Chy4G078830 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 8.3e-24
Identity = 72/211 (34.12%), Postives = 102/211 (48.34%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITF------- 65
           REK+ IP +K  I L G+G ++T V W+DH + A SPTF++ A N VV+ ITF       
Sbjct: 72  REKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFP 131

Query: 66  --------------RILCGMVLAVIYSPS-ATLKVSLMSF-------------------- 125
                           L G   +  YS   A ++ +L  F                    
Sbjct: 132 SKGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFG 191

Query: 126 ----LVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAY 171
               +   CVI V      P L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLG  +
Sbjct: 192 TGQSIYQSCVIQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPW 251

[Close]

BLAST of Chy4G078830 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.9e-22
Identity = 70/211 (33.18%), Postives = 100/211 (47.39%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITF------- 65
           REK+TIP  K  I + G+G + T V W+DH + A SPTF + A N VV+ ITF       
Sbjct: 67  REKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFP 126

Query: 66  --------------RILCGMVLAVIYSPS-ATLKVSL-----------------MSFLV- 125
                             G   +  YS   A ++ +L                 + F++ 
Sbjct: 127 SNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILG 186

Query: 126 ------LECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAY 171
                   CVI V      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LG A+
Sbjct: 187 SGQSIYQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAW 246

[Close]

BLAST of Chy4G078830 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.0e-21
Identity = 65/206 (31.55%), Postives = 96/206 (46.60%), Query Frame = 0


Query: 7   EKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFR------- 66
           EKVTIP  K  I++ G G++ T + + DH+ T  S TFTS   N+++ GITF+       
Sbjct: 80  EKVTIPRGKGYIYMQGGGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIAS 139

Query: 67  -----------ILCGMVLAVIYSPSATLKVSL-----------------MSFL------V 126
                      ++ G   A+I S     + +L                 + F+      +
Sbjct: 140 SSSPTKPAVAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSI 199

Query: 127 LE-CVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFST 171
            E C + + V  Y P    G ITAQG+++P +  GFVF  C V GSG   LG A++ +S 
Sbjct: 200 FEGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSR 259

[Close]

BLAST of Chy4G078830 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 5.6e-20
Identity = 64/208 (30.77%), Postives = 93/208 (44.71%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRILCGMV 65
           REKVTIP +K  I+L G G++ T + ++DH+ T  S TFT+ A ++V+ GITF+    +V
Sbjct: 73  REKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIV 132

Query: 66  -------------------LAVIYSPSATLKVSLM------------------------S 125
                                V  S    L+ +L                          
Sbjct: 133 PNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQ 192

Query: 126 FLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPF 171
            L  EC +N+ +  YAP    G ITA  + +P++  GFVF  C V G G   LG A+   
Sbjct: 193 SLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSN 252

[Close]

BLAST of Chy4G078830 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 8.3e-16
Identity = 41/84 (48.81%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 87  ECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFSTVI 146
           +C IN    A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LG AY PFS VI
Sbjct: 156 DCHINATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVI 215

Query: 147 FHLCFLPSCINPAGWNSWLQVGHE 171
           F    L S ++P GW SW   G+E
Sbjct: 216 FIQTDLSSVVHPEGWYSWHYGGYE 239

[Close]

BLAST of Chy4G078830 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.2e-64
Identity = 134/209 (64.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 7   EKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRIL----- 66
           EKVTIPS+KSCIFLDGSGL+VTE+HWNDHETTAASPTFT+SAQNLVVQGITFR       
Sbjct: 85  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 144

Query: 67  -------------------------CGMV--------------LAVIYSPSATLKVS-LM 126
                                    CG +                  Y       +S   
Sbjct: 145 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 204

Query: 127 SFLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRP 171
             +  ECVIN+PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLG AYRP
Sbjct: 205 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 264

[Close]

BLAST of Chy4G078830 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 6.8e-53
Identity = 115/198 (58.08%), Postives = 132/198 (66.67%), Query Frame = 0


Query: 11  IPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLV-----VQGITFR-ILCGM 70
           IP DKSCIFLDGSG +VTE+ WNDH TTA SPTFTS AQNLV     +  +T R  +   
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVNTYNALGSVTRREDIKPA 61

Query: 71  VLAVIYSPSATLK----------------------------VSLMS----FLVLECVINV 130
           + A+I    A                               + ++S     +  ECVIN+
Sbjct: 62  IAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 121

Query: 131 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFSTVIFHLCFL 171
           P+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLG AYRPFSTVIFHLCFL
Sbjct: 122 PINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFL 181

[Close]

BLAST of Chy4G078830 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 4.1e-50
Identity = 114/211 (54.03%), Postives = 126/211 (59.72%), Query Frame = 0


Query: 21  DGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRIL------------------- 80
           DGSG +VTE+ WNDH TTA SPTFTS AQNLVVQGITFR                     
Sbjct: 85  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAA 144

Query: 81  -----------CGM-----------------------VLAVIYSPSATLKVSLMSFLVL- 140
                      CG                        V+ VI      ++V +    V  
Sbjct: 145 LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCL 204

Query: 141 -------ECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAY 171
                  ECVIN+P+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLG AY
Sbjct: 205 FVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 264

[Close]

BLAST of Chy4G078830 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.7e-43
Identity = 101/209 (48.33%), Postives = 125/209 (59.81%), Query Frame = 0


Query: 7   EKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFR------- 66
           EKVTIP  KSCIF+DG+G +VTE+ WNDH+TTA S TFTS A+NLVV+GI FR       
Sbjct: 82  EKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSATFTSFAENLVVRGIAFRNTYNAPG 141

Query: 67  ------ILCGMVLAVIYSPSATL----------------------------KVSLMS--- 126
                  +   V A+I    A+                              + ++S   
Sbjct: 142 SVRRQEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVIDVISGSG 201

Query: 127 -FLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRP 171
             +  +C+INVP++ YAP+L+ G+ITAQGKENPNE SGFVF  C V G+G V+LG AYRP
Sbjct: 202 QSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRP 261

[Close]

BLAST of Chy4G078830 vs. ExPASy TrEMBL
Match: B9RNC0 (Pectinesterase OS=Ricinus communis OX=3988 GN=RCOM_1346520 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 3.7e-27
Identity = 80/214 (37.38%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRILCGMV 65
           REKVTIP  K CIFL+G+G+++T + W DHE T+ S TFTS   N+V +GITF+    + 
Sbjct: 76  REKVTIPVKKPCIFLEGAGIRLTSIEWGDHEATSTSATFTSYPDNIVAKGITFKNTYNLD 135

Query: 66  LAVI----------YSPSATLKVSLMSF-------------------------------- 125
           +  I           + SA +K    +F                                
Sbjct: 136 ITKINWWGEKIIWRQAVSARIKGEQCAFYKCAFLGTQDTLWDEKGRHYFSNCYIEGAIDF 195

Query: 126 -------LVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLG 171
                  +   CVI+V +  Y P L +G ITAQ KE P  SSGFVF  C+V G+G  FLG
Sbjct: 196 IFGKAQSIYEGCVISVNIGKYPPGL-QGCITAQKKEWPQHSSGFVFKNCVVSGTGKAFLG 255

[Close]

BLAST of Chy4G078830 vs. NCBI nr
Match: XP_011652807.1 (probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 251 bits (641), Expect = 8.60e-81
Identity = 134/209 (64.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 7   EKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRIL----- 66
           EKVTIPS+KSCIFLDGSGL+VTE+HWNDHETTAASPTFT+SAQNLVVQGITFR       
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 67  -------------------------CGMV--------------LAVIYSPSATLKVS-LM 126
                                    CG +                  Y       +S   
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 127 SFLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRP 170
             +  ECVIN+PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLG AYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

[Close]

BLAST of Chy4G078830 vs. NCBI nr
Match: KAE8650757.1 (hypothetical protein Csa_017626 [Cucumis sativus])

HSP 1 Score: 251 bits (641), Expect = 9.88e-80
Identity = 134/209 (64.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 7   EKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRIL----- 66
           EKVTIPS+KSCIFLDGSGL+VTE+HWNDHETTAASPTFT+SAQNLVVQGITFR       
Sbjct: 85  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 144

Query: 67  -------------------------CGMV--------------LAVIYSPSATLKVS-LM 126
                                    CG +                  Y       +S   
Sbjct: 145 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 204

Query: 127 SFLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRP 170
             +  ECVIN+PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLG AYRP
Sbjct: 205 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 264

[Close]

BLAST of Chy4G078830 vs. NCBI nr
Match: KAA0038126.1 (putative pectinesterase 55 [Cucumis melo var. makuwa] >TYK20481.1 putative pectinesterase 55 [Cucumis melo var. makuwa])

HSP 1 Score: 213 bits (542), Expect = 2.36e-66
Identity = 116/198 (58.59%), Postives = 130/198 (65.66%), Query Frame = 0


Query: 11  IPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLV-----VQGITFRI-LCGM 70
           IP DKSCIFLDGSG +VTE+ WNDH TTA SPTFTS AQNLV     +  +T R  +   
Sbjct: 2   IPPDKSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVNTYNALGSVTRREDIKPA 61

Query: 71  VLAVIYSPSATLK----VSLMSFL----------------------------VLECVINV 130
           + A+I    A       + L   L                              ECVIN+
Sbjct: 62  IAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINI 121

Query: 131 PVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFSTVIFHLCFL 170
           P+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLG AYRPFSTVIFHLCFL
Sbjct: 122 PINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFL 181

[Close]

BLAST of Chy4G078830 vs. NCBI nr
Match: XP_016900403.1 (PREDICTED: probable pectinesterase 55 [Cucumis melo])

HSP 1 Score: 204 bits (519), Expect = 1.18e-61
Identity = 114/211 (54.03%), Postives = 126/211 (59.72%), Query Frame = 0


Query: 21  DGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRIL------------------- 80
           DGSG +VTE+ WNDH TTA SPTFTS AQNLVVQGITFR                     
Sbjct: 85  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAA 144

Query: 81  -----------CGM-----------------------VLAVIYSPSATLKVSLMSFLVL- 140
                      CG                        V+ VI      ++V +    V  
Sbjct: 145 LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCL 204

Query: 141 -------ECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAY 170
                  ECVIN+P+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLG AY
Sbjct: 205 FVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 264

[Close]

BLAST of Chy4G078830 vs. NCBI nr
Match: XP_038904330.1 (putative pectinesterase 52 [Benincasa hispida])

HSP 1 Score: 203 bits (517), Expect = 2.50e-61
Identity = 111/214 (51.87%), Postives = 128/214 (59.81%), Query Frame = 0


Query: 2   LYFNREKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRIL 61
           L   REKV IP +KSCIFLDGSG K TE+ WND+ TTA SPTFTS A+NLVVQGITFR  
Sbjct: 139 LLMEREKVMIPPEKSCIFLDGSGRKTTEIQWNDYITTATSPTFTSFAENLVVQGITFRNT 198

Query: 62  ------------------------------CGMV--------------LAVIYSPSATLK 121
                                         CG +                  Y       
Sbjct: 199 HNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDV 258

Query: 122 VS-LMSFLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLG 170
           +S     +  +CVIN+P+N YAP+LN G+ITAQGK N +ES+GFVF+ C V GSGNVFLG
Sbjct: 259 ISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLG 318

[Close]

BLAST of Chy4G078830 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 5.9e-25
Identity = 72/211 (34.12%), Postives = 102/211 (48.34%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITF------- 65
           REK+ IP +K  I L G+G ++T V W+DH + A SPTF++ A N VV+ ITF       
Sbjct: 72  REKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFP 131

Query: 66  --------------RILCGMVLAVIYSPS-ATLKVSLMSF-------------------- 125
                           L G   +  YS   A ++ +L  F                    
Sbjct: 132 SKGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFG 191

Query: 126 ----LVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAY 171
               +   CVI V      P L  G+ITAQG+ NP +++GF+F+ C+V G+G  FLG  +
Sbjct: 192 TGQSIYQSCVIQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPW 251

[Close]

BLAST of Chy4G078830 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 4.2e-23
Identity = 70/211 (33.18%), Postives = 100/211 (47.39%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITF------- 65
           REK+TIP  K  I + G+G + T V W+DH + A SPTF + A N VV+ ITF       
Sbjct: 67  REKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFP 126

Query: 66  --------------RILCGMVLAVIYSPS-ATLKVSL-----------------MSFLV- 125
                             G   +  YS   A ++ +L                 + F++ 
Sbjct: 127 SNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILG 186

Query: 126 ------LECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAY 171
                   CVI V      P +  G+ITAQG+ N N+++GFVF+ C+V G G  +LG A+
Sbjct: 187 SGQSIYQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAW 246

[Close]

BLAST of Chy4G078830 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 3.6e-22
Identity = 65/206 (31.55%), Postives = 96/206 (46.60%), Query Frame = 0


Query: 7   EKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFR------- 66
           EKVTIP  K  I++ G G++ T + + DH+ T  S TFTS   N+++ GITF+       
Sbjct: 80  EKVTIPRGKGYIYMQGGGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIAS 139

Query: 67  -----------ILCGMVLAVIYSPSATLKVSL-----------------MSFL------V 126
                      ++ G   A+I S     + +L                 + F+      +
Sbjct: 140 SSSPTKPAVAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSI 199

Query: 127 LE-CVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFST 171
            E C + + V  Y P    G ITAQG+++P +  GFVF  C V GSG   LG A++ +S 
Sbjct: 200 FEGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSR 259

[Close]

BLAST of Chy4G078830 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.0e-21
Identity = 64/208 (30.77%), Postives = 93/208 (44.71%), Query Frame = 0


Query: 6   REKVTIPSDKSCIFLDGSGLKVTEVHWNDHETTAASPTFTSSAQNLVVQGITFRILCGMV 65
           REKVTIP +K  I+L G G++ T + ++DH+ T  S TFT+ A ++V+ GITF+    +V
Sbjct: 73  REKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIV 132

Query: 66  -------------------LAVIYSPSATLKVSLM------------------------S 125
                                V  S    L+ +L                          
Sbjct: 133 PNNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQ 192

Query: 126 FLVLECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPF 171
            L  EC +N+ +  YAP    G ITA  + +P++  GFVF  C V G G   LG A+   
Sbjct: 193 SLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSN 252

[Close]

BLAST of Chy4G078830 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 85.1 bits (209), Expect = 5.9e-17
Identity = 41/84 (48.81%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 87  ECVINVPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGGAYRPFSTVI 146
           +C IN    A A  ++ G+ITAQG+ + ++ SGFVFLR  V GS +V+LG AY PFS VI
Sbjct: 156 DCHINATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVI 215

Query: 147 FHLCFLPSCINPAGWNSWLQVGHE 171
           F    L S ++P GW SW   G+E
Sbjct: 216 FIQTDLSSVVHPEGWYSWHYGGYE 239

The following BLAST results are available for this feature:

BLAST of Chy4G078830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4PSN0	8.3e-24	34.12	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
Q3E9D3	5.9e-22	33.18	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
O64479	5.0e-21	31.55	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
Q4PSQ5	5.6e-20	30.77	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
O04953	8.3e-16	48.81	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of Chy4G078830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB12	2.2e-64	64.11	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1	[more]
A0A5D3DA76	6.8e-53	58.08	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550...	[more]
A0A1S4DWQ1	4.1e-50	54.03	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1	[more]
A0A6J1DWQ8	1.7e-43	48.33	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1	[more]
B9RNC0	3.7e-27	37.38	Pectinesterase OS=Ricinus communis OX=3988 GN=RCOM_1346520 PE=4 SV=1	[more]

BLAST of Chy4G078830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652807.1	8.60e-81	64.11	probable pectinesterase 55 [Cucumis sativus]	[more]
KAE8650757.1	9.88e-80	64.11	hypothetical protein Csa_017626 [Cucumis sativus]	[more]
KAA0038126.1	2.36e-66	58.59	putative pectinesterase 55 [Cucumis melo var. makuwa] >TYK20481.1 putative pecti...	[more]
XP_016900403.1	1.18e-61	54.03	PREDICTED: probable pectinesterase 55 [Cucumis melo]	[more]
XP_038904330.1	2.50e-61	51.87	putative pectinesterase 52 [Benincasa hispida]	[more]

BLAST of Chy4G078830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24130.1	5.9e-25	34.12	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	4.2e-23	33.18	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	3.6e-22	31.55	Pectin lyase-like superfamily protein	[more]
AT2G47280.1	4.0e-21	30.77	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	5.9e-17	48.81	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 80..169 e-value: 3.5E-15 score: 58.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 3..74 e-value: 7.5E-6 score: 27.5
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 102..165 e-value: 5.4E-10 score: 38.5
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 87..170 coord: 6..65
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 87..170 coord: 6..65
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 6..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G078830.1	Chy4G078830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity