Chy2G037510 (gene) Cucumber (hystrix) v1

BLAST of Chy2G037510 vs. ExPASy Swiss-Prot
Match: Q9SC88 (Gamma-tubulin complex component 4 homolog OS=Medicago truncatula OX=3880 GN=85P PE=2 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 2.9e-310
Identity = 552/758 (72.82%), Postives = 631/758 (83.25%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIID R+       +NL  + PIS E TFKLA DISF++PS+R+L
Sbjct: 1   MLHELLLALLGYTGDLIIDRRD-------NNLSANTPISDECTFKLAPDISFIDPSDREL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           I+RII LGFYYREL+RF+ KSRNL+WIRS   + L N         E PSVYRRA+ANGI
Sbjct: 61  IERIITLGFYYRELERFSAKSRNLNWIRSENANPLENK--------EKPSVYRRALANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEIL++Y S++LH+EQ LLSET+PILA VTQGL+KFF L PPL++LI +IE  DIRGG+L
Sbjct: 121 VEILAVYSSSILHIEQLLLSETMPILATVTQGLNKFFSLLPPLYELILKIERGDIRGGEL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHK+CHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+L+D+HGEFFI RQ+ R+ 
Sbjct: 181 LNLLHKKCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILEDRHGEFFISRQEGRDV 240

Query: 241 NQGSSVQDTSEKLGRLST-DESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLR 300
              SS Q+ SEKL RLST D SL+DWH+GFHI LDMLP+YI MRVAESILFAGKA+RVLR
Sbjct: 241 ENSSSHQEISEKLSRLSTADASLSDWHMGFHISLDMLPEYIPMRVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIE 360
           NPS +F  Q   +  +   P+    + G    F  Q+EP + T +  E+LL QSEADKIE
Sbjct: 301 NPSPSFLSQ---DDVYPQEPKRFPKIHGFEGRFNFQREPIINTGMRVEDLLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            MLLDLKESSEFHKRSFE AVDSI+AIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 NMLLDLKESSEFHKRSFECAVDSIQAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKP 480
           FQCFLEESR LMRLPPRQSTAEADLM+PFQLA+ KT  EED+YFS+VSLRMPS+G+ VKP
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKP 480

Query: 481 AQGDLKE--KPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCR 540
           +  ++ +      DG  G ++SN S +MS+DGWDG++LEY I+WPL LFFTQEVLS+Y +
Sbjct: 481 SLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLK 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGS-ISLQRRQRFRRMWRVREH 600
           VFQYLLRLKRTQMELEK WAS+MHQ H+ FA N+K+  D S I+ QR QRFR MWRVREH
Sbjct: 541 VFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQWNILQ HIQDSHDFTELVGFHQEYLSALISQ+FLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILDGIMKLCLQFCW+IENQD+ S+ SELEH+ EEFNKKSNSLYTILRSSRL GSQR PF
Sbjct: 661 RILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 755
           LRRFL+RLN NSFFE+TA+ V+NVVRPRP   P LNQ+
Sbjct: 721 LRRFLLRLNLNSFFESTAKEVMNVVRPRP-TFPGLNQR 739

BLAST of Chy2G037510 vs. ExPASy Swiss-Prot
Match: Q9M350 (Gamma-tubulin complex component 4 OS=Arabidopsis thaliana OX=3702 GN=GCP4 PE=2 SV=2)

HSP 1 Score: 1058.9 bits (2737), Expect = 2.7e-308
Identity = 556/750 (74.13%), Postives = 626/750 (83.47%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE   +LGL     D+P+S E TFKLA DISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRS-GKESSLANSTELSKDKIE-NPSVYRRAIAN 120
           I+R+I LGFYYRELDRFA KSRNLSWIRS      L  + ELSK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGG 180
           GI EILS+YRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL+++I EIE DDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYG+LQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETNQGSSVQDTSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRV 300
           + +  SS ++ SEKL R S  E SLTDWH GFHI LDMLPDYI MR+ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S     R    ++G   +   + E  +   LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  VDS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKV 480
           DFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT +EED+YFSRVSLRMPSFGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KPAQGDL-KEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYC 540
           + +Q D+ + K  + G      +NL+ D S+DGWD ++LEY +DWP+QLFFTQEVLSKY 
Sbjct: 481 RSSQADMVRSKVSLTGK-----ANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYL 540

Query: 541 RVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREH 600
           +VFQYL+RLKRTQMELEKSWAS+MHQDH + A +RK   +GS S QRRQ  R MWRVREH
Sbjct: 541 KVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQW +LQ HI DS DFTELVGFHQEYLSALISQSFLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILD IMKLCLQFCW+IENQ+S+ + SELE++ EEFNKKSNSLYTILRSS+L GSQRAPF
Sbjct: 661 RILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRPRPA 747
           LRRFL+RLNFNSF+EATARGVLNVVR RPA
Sbjct: 721 LRRFLLRLNFNSFYEATARGVLNVVRQRPA 742

BLAST of Chy2G037510 vs. ExPASy Swiss-Prot
Match: Q9UGJ1 (Gamma-tubulin complex component 4 OS=Homo sapiens OX=9606 GN=TUBGCP4 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-83
Identity = 225/749 (30.04%), Postives = 366/749 (48.87%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           + R+  LG  Y     F      +       +    + ++  +  +    +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEF------IEQYTGHVQQQDHHPSQQGQGGLH--GIYLRAFCTGL 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
             +L  YR A+L +EQ+ L +    ++ V   LD+F +LFP +  ++ +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++GLL D+H EFFI +Q     
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFI-KQGPSSG 240

Query: 241 NQGSSVQDTSEKLG---------------RLSTDESLTDWHL-GFHIYLDMLPDYIHMRV 300
           N  +  ++  E LG               RL  +E++    L  F + +++LP YI +RV
Sbjct: 241 NVSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRV 300

Query: 301 AESILFAGKAIRVLRNPSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKL 360
           AE ILF G+++++  N +                                         L
Sbjct: 301 AEKILFVGESVQMFENQN---------------------------------------VNL 360

Query: 361 TGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGH 420
           T +  +L+++ D   A L  LK+   F    FE  VD IR+  A HLW+L+V  +DL G 
Sbjct: 361 TRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQ 420

Query: 421 LKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFS 480
           LK +KD++LL +G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   ++D    
Sbjct: 421 LKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLLP 480

Query: 481 RVSLRMPSFGVKVKPAQGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQ 540
            + L +   G + K      +E P  + +P  A ++        GW  + L Y + WPL 
Sbjct: 481 LLHLTIEYHGKEHKADATQAREGPSRETSPREAPAS--------GWAALGLSYKVQWPLH 540

Query: 541 LFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRR 600
           + FT  VL KY  VF+YLL ++R Q EL+  WA  M + H                L+  
Sbjct: 541 ILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKH----------------LKSN 600

Query: 601 QRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSAL 660
           Q     WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L   I  + DF  +   H  +LS L
Sbjct: 601 QTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNL 655

Query: 661 ISQSFLDIGSVSRILDGIMKLCLQFCWSIENQDSSSD---PSELEHLTEEFNKKSNSLYT 720
           ++QSF+ +  V   L+ I+ LC  FC  +       D    ++L  L + F+++S+ L+ 
Sbjct: 661 LAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFK 655

Query: 721 ILRSSRLVGSQRAPFLRRFLMRLNFNSFF 731
           IL S R    Q    L + L+RL++N ++
Sbjct: 721 ILSSVR--NHQINSDLAQLLLRLDYNKYY 655

BLAST of Chy2G037510 vs. ExPASy Swiss-Prot
Match: Q9D4F8 (Gamma-tubulin complex component 4 OS=Mus musculus OX=10090 GN=Tubgcp4 PE=1 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 2.4e-83
Identity = 225/749 (30.04%), Postives = 365/749 (48.73%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           + R+  LG  Y     F      +       +    +  +  +  +    +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEF------IEQYTGHVQQQDHHPPQQGQGGLH--GIYLRAFCTGL 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
             +L  YR A+L +EQ+ L++    ++ V   LD+F +LFP +  ++ +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLADPHLSISHVNYSLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++GLL D+H EFFI +Q     
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFI-KQGPSSG 240

Query: 241 NQGSSVQDTSEKLG---------------RLSTDESLTDWHL-GFHIYLDMLPDYIHMRV 300
              + +++  E LG               RL  +E++    L  F + +++LP YI +RV
Sbjct: 241 TLSAQLEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRV 300

Query: 301 AESILFAGKAIRVLRNPSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKL 360
           AE ILF G+++++  N +                                         L
Sbjct: 301 AEKILFVGESVQMFENQN---------------------------------------VNL 360

Query: 361 TGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGH 420
           T +  +L+++ D   A L  LK+   F    FE  VD IR+  A HLW+L+V  +DL G 
Sbjct: 361 TRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQ 420

Query: 421 LKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFS 480
           LK +KD++LL +G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   ++D    
Sbjct: 421 LKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLLP 480

Query: 481 RVSLRMPSFGVKVKPAQGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQ 540
            + L +   G   K      +E P  + +P  A S+        GW  + L Y + WPL 
Sbjct: 481 LLHLTIEYHGKDHKADATQPREVPSRETSPREAPSS--------GWAALGLSYKVQWPLH 540

Query: 541 LFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRR 600
           + FT  VL KY  VF+YLL ++R Q EL+  WA  M + H                L+  
Sbjct: 541 ILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKH----------------LKSN 600

Query: 601 QRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSAL 660
           Q     WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L   I  + DF  +   H  +LS L
Sbjct: 601 QTDAVKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNL 655

Query: 661 ISQSFLDIGSVSRILDGIMKLCLQFCWSIENQDSSSD---PSELEHLTEEFNKKSNSLYT 720
           ++QSF+ +  V   L+ I+ LC  FC  +       D    ++L  L + F+++S+ L+ 
Sbjct: 661 LAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFK 655

Query: 721 ILRSSRLVGSQRAPFLRRFLMRLNFNSFF 731
           IL S R    Q    L + L+RL++N ++
Sbjct: 721 ILSSVR--NHQINSDLAQLLLRLDYNKYY 655

BLAST of Chy2G037510 vs. ExPASy Swiss-Prot
Match: Q9VKU7 (Gamma-tubulin complex component 4 homolog OS=Drosophila melanogaster OX=7227 GN=Grip75 PE=1 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 3.3e-40
Identity = 173/704 (24.57%), Postives = 303/704 (43.04%), Query Frame = 0

Query: 52  FLEPSERDLIQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSV 111
           F+ P ER++   II +   Y+E+++F   S      +S     L +S             
Sbjct: 31  FIHPCEREIFMDIIKIIKVYQEVEQFTHSSGR----KSDTHGELPDSLH---------GY 90

Query: 112 YRRAIANGIVEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIE 171
           Y   +A GI   L  Y + +  +E+  L      L+ V   L   F L   +  LI EI 
Sbjct: 91  YLLNLAKGIEMALEEYYAEIGRLEKYCLGNERNSLSYVYNALYAKFPLLVFMRNLITEIH 150

Query: 172 HDDIRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFF 231
             ++RG  LL+ LH++C  G  +L+  I+ ++       ++ LA W+++G++ D H EFF
Sbjct: 151 VLNLRGCVLLHNLHQQCEHGDIQLEKAIKIIMKPVKNAFFSSLAHWLLFGVIDDVHSEFF 210

Query: 232 IRRQDDRETNQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFA 291
           I +    +   GSS    S     LS +++  D+   + + +  LP +  + +AE +LF 
Sbjct: 211 I-KFTPTDAVDGSSF-SKSATCSLLSAEKNPEDYIWQYEVNMSQLPGFFSIVLAEKVLFV 270

Query: 292 GKAIRVLRNPSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEEL-- 351
           G+ + V                      ++  NVK   +  PL  +     +L  +++  
Sbjct: 271 GQTVLVF---------------------KMGRNVKVKNKTDPLAAK---LAELDSDDIYQ 330

Query: 352 LLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALK 411
           L      +   M++DL      +    E  +  I+   ++ L ++ V   DL   +  +K
Sbjct: 331 LWSGRESEFFKMVVDLSNEDTINVFRLEKVIIDIKNYVSARLSEIAVNEVDLERQMGLIK 390

Query: 412 DYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLR 471
           D+FLL +G+F+  F                         Q+  T  T  E+R F  V+ R
Sbjct: 391 DFFLLGRGEFYLEFCS-----------------------QMVGTMETYREER-FKNVT-R 450

Query: 472 MPSFGVKVKPAQGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQ 531
                  V     DL +  +I         + S     +   G+SL+Y  +WPL L F+ 
Sbjct: 451 SFELAATVTGITDDLDKFSLICQRSTSEPDDTS---DFNFLQGLSLKYEYEWPLNLLFSP 510

Query: 532 EVLSKYCRVFQYLLRLKRTQMELEKSWA--SLMHQDHADFANNRKAQFDGSISLQRRQRF 591
             + +Y  +F++LL ++  Q E+++ WA  +   +   D   N K               
Sbjct: 511 TTIERYNNIFRFLLIIRTYQYEIQRVWAKQTWRAKSAKDVPPNNK--------------- 570

Query: 592 RRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQ 651
             +  +R ++ F + N+Q+YIQVDV+ESQ+ IL + I+   DF  +   H  +L+ ++S 
Sbjct: 571 --IITLRNYLMFFLNNMQYYIQVDVLESQFGILMNVIKSRSDFEVIQRAHTVFLANVLSH 630

Query: 652 SFL---------DIGSVSR--ILDGIMKL---CLQFCWSIENQDSSSD-PSELEHLTEEF 711
            FL           GS +R  I   ++KL   C +F    + +D S D   E++ L E F
Sbjct: 631 CFLLNESETQLNVTGSQNRNPIYGTLLKLFGICEKFAHMTQTKDPSDDLEDEVDQLNESF 645

Query: 712 NKKSNSLYTIL---RSSRLVGSQRAPFLRRFLMRLNFNSFFEAT 734
             +  SL  +L   +S+  +G      L + L+RL+FN +F A+
Sbjct: 691 GVQIASLIQLLVDVKSASCLGP-----LSQLLLRLDFNCWFSAS 645

BLAST of Chy2G037510 vs. ExPASy TrEMBL
Match: A0A5A7SW23 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00060 PE=3 SV=1)

HSP 1 Score: 1468.8 bits (3801), Expect = 0.0e+00
Identity = 744/755 (98.54%), Postives = 747/755 (98.94%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL+NLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG ESSLANSTE SKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIE DDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGAL-SNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGAL SNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFANNR AQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 755
           FLMRLNFNSFFEATARGVLNVVRPRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of Chy2G037510 vs. ExPASy TrEMBL
Match: A0A5A7VFR5 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00280 PE=3 SV=1)

HSP 1 Score: 1457.2 bits (3771), Expect = 0.0e+00
Identity = 741/755 (98.15%), Postives = 743/755 (98.41%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL+NLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG ESSLA STE SKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLI EIE DDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSNMPRLPLNVKGN RNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGAL-SNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGAL SNLSLDMS DGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFAN R AQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 755
           FLMRLNFNSFFEATARGVLNVVRPRP+ALPVLNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPSALPVLNQQ 755

BLAST of Chy2G037510 vs. ExPASy TrEMBL
Match: A0A6J1F2V2 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111441869 PE=3 SV=1)

HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 705/754 (93.50%), Postives = 722/754 (95.76%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGL++L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRS   SSL NS E SKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIE DDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           +QGS+VQ  SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPL +KGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK   DGNPGGALSNLS DMSLDGWD V+LEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+ NR A+FDGSIS Q+RQRFR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVV PRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of Chy2G037510 vs. ExPASy TrEMBL
Match: A0A6J1J9A7 (Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111482469 PE=3 SV=1)

HSP 1 Score: 1384.0 bits (3581), Expect = 0.0e+00
Identity = 699/754 (92.71%), Postives = 720/754 (95.49%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH+N LGL++L  DAPISGEPTFKLA DISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS   SSL NS E SKDK E PSVYRRAIANGI
Sbjct: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIE DDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           +QGS+VQ  SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPL +KG TRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMP FGVKVK A
Sbjct: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEKP  DGNPGGALSNLS DMSLDGW+GV+LEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+ NR A+FDGSIS Q+RQ FR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+ SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVVR RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 754

BLAST of Chy2G037510 vs. ExPASy TrEMBL
Match: A0A6J1DP06 (Gamma-tubulin complex component OS=Momordica charantia OX=3673 GN=LOC111022444 PE=3 SV=1)

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 701/754 (92.97%), Postives = 720/754 (95.49%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL++L  DAPISGE TFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLPPDAPISGESTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG  SSLA S E SK K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLAKSAESSKAKTENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIE DDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQD HGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDNHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
            QGSSVQD SEKLGRLSTD+SLTDWHLGFHIYLDMLPDYIHM VAESILFAGKAIRVLRN
Sbjct: 241 EQGSSVQDMSEKLGRLSTDDSLTDWHLGFHIYLDMLPDYIHMWVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAG QSHSN+ R+PLN+KGNTRNFPLQKEPF+ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGYQSHSNISRVPLNIKGNTRNFPLQKEPFIATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKE+SEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKEASEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YFSRVSLRMPSFGV+VK  
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVRVKST 480

Query: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           Q    EK   DGNPG ALSNLS DMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 Q----EKTYTDGNPGAALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFA NR+AQ DGS+S  +RQRFRRMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFAQNRRAQRDGSVSSHQRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDHIQDSHDFT+LVG+HQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTDLVGYHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQDSSS+PSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDSSSNPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVVR RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRARPAALPVLNQQ 750

BLAST of Chy2G037510 vs. NCBI nr
Match: XP_011660150.1 (gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_011660151.1 gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_031744625.1 gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >KGN66813.2 hypothetical protein Csa_007116 [Cucumis sativus])

HSP 1 Score: 1481 bits (3834), Expect = 0.0
Identity = 747/754 (99.07%), Postives = 747/754 (99.07%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG ESSLANSTE SKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSE VPILAIVTQGLDKFFVLFPPLHQLIREIE DDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSEMVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYF RVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFCRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of Chy2G037510 vs. NCBI nr
Match: KAA0035230.1 (gamma-tubulin complex component 4-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1472 bits (3812), Expect = 0.0
Identity = 744/755 (98.54%), Postives = 747/755 (98.94%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL+NLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG ESSLANSTE SKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIE DDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALS-NLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALS NLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFANNR AQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           FLMRLNFNSFFEATARGVLNVVRPRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of Chy2G037510 vs. NCBI nr
Match: KAA0067222.1 (gamma-tubulin complex component 4-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1461 bits (3782), Expect = 0.0
Identity = 741/755 (98.15%), Postives = 743/755 (98.41%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL+NLLVDAPISGEPTFKLASDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG ESSLA STE SKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLI EIE DDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSNMPRLPLNVKGN RNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALS-NLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGDLKEKPIIDGNPGGALS NLSLDMS DGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFAN R AQFDGSISLQRRQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           FLMRLNFNSFFEATARGVLNVVRPRP+ALPVLNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPSALPVLNQQ 755

BLAST of Chy2G037510 vs. NCBI nr
Match: XP_038879485.1 (gamma-tubulin complex component 4 homolog [Benincasa hispida])

HSP 1 Score: 1444 bits (3737), Expect = 0.0
Identity = 726/754 (96.29%), Postives = 737/754 (97.75%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLL+LLGYTGDLIIDEREHYNSLGL++L +DAPISGEPTF LA DISFLEPSERDL
Sbjct: 1   MLHELLLSLLGYTGDLIIDEREHYNSLGLNHLPLDAPISGEPTFTLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSG  SSLANSTE SKDKIENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKF+VLFPPLHQLI EIEHDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFYVLFPPLHQLILEIEHDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           NQGSSVQD SEKLGRLSTDESLTDWHLGFHI LDMLPDYIHMRVAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDVSEKLGRLSTDESLTDWHLGFHICLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPLN+KGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEF KRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFRKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEKP IDGNPGGALSNL LDMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPFIDGNPGGALSNLPLDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFANNR AQFDGSIS Q+RQRFRRMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISPQQRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWS+ENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSLENQDGSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of Chy2G037510 vs. NCBI nr
Match: XP_022934806.1 (gamma-tubulin complex component 4 homolog [Cucurbita moschata] >XP_022934807.1 gamma-tubulin complex component 4 homolog [Cucurbita moschata])

HSP 1 Score: 1400 bits (3624), Expect = 0.0
Identity = 705/754 (93.50%), Postives = 722/754 (95.76%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGL++L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGKESSLANSTELSKDKIENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRS   SSL NS E SKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIE DDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 NQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300
           +QGS+VQ  SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPL +KGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKPA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMPSFGVKVK A
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK   DGNPGGALSNLS DMSLDGWD V+LEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+ NR A+FDGSIS Q+RQRFR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQD SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVV PRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of Chy2G037510 vs. TAIR 10
Match: AT3G53760.1 (GAMMA-TUBULIN COMPLEX PROTEIN 4 )

HSP 1 Score: 1058.9 bits (2737), Expect = 1.9e-309
Identity = 556/750 (74.13%), Postives = 626/750 (83.47%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE   +LGL     D+P+S E TFKLA DISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRS-GKESSLANSTELSKDKIE-NPSVYRRAIAN 120
           I+R+I LGFYYRELDRFA KSRNLSWIRS      L  + ELSK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIEHDDIRGG 180
           GI EILS+YRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL+++I EIE DDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYG+LQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETNQGSSVQDTSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRV 300
           + +  SS ++ SEKL R S  E SLTDWH GFHI LDMLPDYI MR+ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S     R    ++G   +   + E  +   LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  VDS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKV 480
           DFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT +EED+YFSRVSLRMPSFGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KPAQGDL-KEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYC 540
           + +Q D+ + K  + G      +NL+ D S+DGWD ++LEY +DWP+QLFFTQEVLSKY 
Sbjct: 481 RSSQADMVRSKVSLTGK-----ANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYL 540

Query: 541 RVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREH 600
           +VFQYL+RLKRTQMELEKSWAS+MHQDH + A +RK   +GS S QRRQ  R MWRVREH
Sbjct: 541 KVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQW +LQ HI DS DFTELVGFHQEYLSALISQSFLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILD IMKLCLQFCW+IENQ+S+ + SELE++ EEFNKKSNSLYTILRSS+L GSQRAPF
Sbjct: 661 RILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRPRPA 747
           LRRFL+RLNFNSF+EATARGVLNVVR RPA
Sbjct: 721 LRRFLLRLNFNSFYEATARGVLNVVRQRPA 742

BLAST of Chy2G037510 vs. TAIR 10
Match: AT5G06680.1 (spindle pole body component 98 )

HSP 1 Score: 109.8 bits (273), Expect = 9.9e-24
Identity = 119/477 (24.95%), Postives = 193/477 (40.46%), Query Frame = 0

Query: 175 IRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRR 234
           +RGG +   +H     G P +   +  LL      ++  + SW++ G L+D  GEFF+  
Sbjct: 334 LRGGAMAGAIHLHAQHGDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVG 393

Query: 235 QDDRETNQGSSVQDTSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKA 294
           Q                K+  L        W  G+ ++  MLP +I   +A+ IL  GK+
Sbjct: 394 Q--------------PVKVDLL--------WREGYKLHPAMLPSFISPSLAQRILRTGKS 453

Query: 295 IRVLRNPSNAF-WCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQS 354
           I  LR   +   W   A   + ++         G T            T+  G   L   
Sbjct: 454 INFLRVCCDDHGWADAASEAAAAS---------GTT------------TRRGG---LGYG 513

Query: 355 EADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFL 414
           E D +E ++      +E  KR     +D        HL  ++  R     H  A+K Y L
Sbjct: 514 ETDALEHLV------TEAAKR-----ID-------KHLLDVLYKRYKFKEHCLAIKRYLL 573

Query: 415 LAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSF 474
           L +GDF Q       +LM +   + +  A+ +  F+LA           F   ++R  + 
Sbjct: 574 LGQGDFVQ-------YLMDIVGPKLSEPANNISSFELAG----------FLEAAIRASNA 633

Query: 475 GVKVKPAQGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLS 534
               +     L+ K +  G+               GWD  SLEY    PL   FT+ VLS
Sbjct: 634 QYDDRDMLDRLRVKMMPHGSGD------------RGWDVFSLEYEARVPLDTVFTESVLS 693

Query: 535 KYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRV 594
           KY RVF +L +LKR +  L   W ++         +N   +   S+ LQ     RR   +
Sbjct: 694 KYLRVFNFLWKLKRVEHALIGIWKTMKPN---CITSNSFVKLQSSVKLQLLSALRRCQVL 714

Query: 595 REHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFL 651
              M   + N Q+YI  +V+E  W+     ++ + D  +L+  H++YL+A++ +S L
Sbjct: 754 WNEMNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLL 714

BLAST of Chy2G037510 vs. TAIR 10
Match: AT5G17410.2 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 84/362 (23.20%), Postives = 151/362 (41.71%), Query Frame = 0

Query: 380 VDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQST 439
           + +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +R  +     + +
Sbjct: 342 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 401

Query: 440 AE-----ADLMIPFQLAATKTTSEE-DRYFSRVSLRMPSFGVKVKPAQGDLKEKPIIDGN 499
            E      DL +    AA     E+      R SL + + G+  K    +  E P     
Sbjct: 402 VEKLQSLLDLALRTTAAAADPRHEDLTCCVDRASL-LTTLGMH-KDTDSNSIEDP----- 461

Query: 500 PGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELE 559
                      MS+ G +  SL Y + WPL +  +++ LSKY  +F++L   K  + +L 
Sbjct: 462 -----------MSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLC 521

Query: 560 KSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFLIRNLQFYIQVDVI 619
            +W   +HQ      +   A    S+  +   +F             I +L  Y+  +V+
Sbjct: 522 GAWQ--IHQGIRSMNSKGTAILRSSLLCRSMLKF-------------ISSLLHYLTFEVL 581

Query: 620 ESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC--- 679
           E  W+++ D +Q +    E++  H  +L   +    L +  V + ++ +  +CLQ+    
Sbjct: 582 EPNWHVMHDRLQSTRSVDEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAAT 641

Query: 680 -WSIENQ---DSSSDPSE-----------LEHLTEEFNKKSNSLYTILRSSRLVGSQRAP 718
            W I +    +S S P +           + +   EFN +  SL  +L      GSQ  P
Sbjct: 642 QWLISSSIDINSQSHPQKTMIRDTTVTESIFNFEREFNSELQSLGPVLSK----GSQAEP 666

BLAST of Chy2G037510 vs. TAIR 10
Match: AT5G17410.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 84/362 (23.20%), Postives = 151/362 (41.71%), Query Frame = 0

Query: 380 VDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQST 439
           + +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +R  +     + +
Sbjct: 341 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 400

Query: 440 AE-----ADLMIPFQLAATKTTSEE-DRYFSRVSLRMPSFGVKVKPAQGDLKEKPIIDGN 499
            E      DL +    AA     E+      R SL + + G+  K    +  E P     
Sbjct: 401 VEKLQSLLDLALRTTAAAADPRHEDLTCCVDRASL-LTTLGMH-KDTDSNSIEDP----- 460

Query: 500 PGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELE 559
                      MS+ G +  SL Y + WPL +  +++ LSKY  +F++L   K  + +L 
Sbjct: 461 -----------MSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLC 520

Query: 560 KSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFLIRNLQFYIQVDVI 619
            +W   +HQ      +   A    S+  +   +F             I +L  Y+  +V+
Sbjct: 521 GAWQ--IHQGIRSMNSKGTAILRSSLLCRSMLKF-------------ISSLLHYLTFEVL 580

Query: 620 ESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC--- 679
           E  W+++ D +Q +    E++  H  +L   +    L +  V + ++ +  +CLQ+    
Sbjct: 581 EPNWHVMHDRLQSTRSVDEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAAT 640

Query: 680 -WSIENQ---DSSSDPSE-----------LEHLTEEFNKKSNSLYTILRSSRLVGSQRAP 718
            W I +    +S S P +           + +   EFN +  SL  +L      GSQ  P
Sbjct: 641 QWLISSSIDINSQSHPQKTMIRDTTVTESIFNFEREFNSELQSLGPVLSK----GSQAEP 665

BLAST of Chy2G037510 vs. TAIR 10
Match: AT3G43610.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 53.9 bits (128), Expect = 6.4e-07
Identity = 70/347 (20.17%), Postives = 130/347 (37.46%), Query Frame = 0

Query: 401  LNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED 460
            L  HL AL+ Y  +   D+   F+    H   L        A++    + +  +++ E D
Sbjct: 879  LQEHLLALRRYHFMELADWADVFVVSLWHHKWLVTEADKRIAEIQGFLESSIQRSSCERD 938

Query: 461  RYFSRVSLRMPSFGVKVKPAQGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYID 520
                R+ L      + + P+                        + +  +D + L Y +D
Sbjct: 939  ICKDRIFLYKRQGTMHIPPS-----------------------TIGVRSFDFLRLGYRVD 998

Query: 521  WPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSIS 580
            WP+ +  T + L+ Y  VF +L+++K     L   W SL    H       K      I 
Sbjct: 999  WPISIILTCDALTAYADVFSFLVQVKLAAYVLTDVWCSLKDVRHMMHEKKEK------IL 1058

Query: 581  LQRRQRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDS-HDFTELVGFHQE 640
             Q  +    + ++R  +   +  LQ Y+  ++    W+     +++   D  +L   H  
Sbjct: 1059 KQELRWLNILMKLRHQVNHFVTALQQYVHSELSHVSWSKFLHSLKNKVKDMMDLESVHMA 1118

Query: 641  YLSALISQSFL--DIGSVSRILDGIMKLCLQFCWSIENQDSSSD--------------PS 700
            YLS  +   FL  +   +S I++ I++  L F   +     S+D               S
Sbjct: 1119 YLSEALRICFLSDETQIISNIIENILQCALDFRSCLPRGIQSTDRVPNDSWTKTLGINTS 1178

Query: 701  ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRFLMRLNFNSFF 731
            ++  + + F+K+   L+     S   G      L RF   LNFN ++
Sbjct: 1179 QVMMVKQNFDKELKELHKCHLRSPKHGKYG---LSRFWDYLNFNLYY 1193