Chy1G018800 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G018800
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	SpoU_methylase domain-containing protein
Location	chrH01: 24162382 .. 24164982 (-)
RNA-Seq Expression	Chy1G018800
Synteny	Chy1G018800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGTTAATGGACACAACAACAGCCACTTTCAGAACTTTAAACCCATTTATCCCTTTTCAACTACGCTCATCCAGGTCTCTTCATCTTCTTCGCTCTCACTCCTTCTCCCTTTTCACCAACAAACACCACAATCCATCTTCTTCATCCTCCCGCCGGTTACTCCCCTTCTGCTCTTCATGTAAGTATATATGATATATCTGTAATCAAACGTTCTTACATCCTATGGACTCTGTAATCTCTGCTGTGAATTGGGTTGAAGTTATTTTTTTCAGTATGATTTTGCTGTGTTCATGGCGCAGCTGACAGAGTGGAGGGTTCTGGTTCCCTACCGAGCGGTGTTGGTGAGGCCGTCAATGGGGTCTCGCATCCAAAGCTTCTTCAAGTTGTATTGGTGTCTCCCTTGGTAATTGCTTAGTTGATTATTTTTCTTGCTTTTCGTTTCTGCATTTTTTTTTTCTAGTAATCGCTATTTTTAGTTATTTTTTGTGTTCTTATCTATATTTGGTGGAGTTGTATGGCTTGATTTAATCTTTTGAATAAGTTTCACTTTGCTTTAAGGTTTTGTTTGATTGTCAAGGATGGTATTTCATATTATTGGAATTTTTGATCAAATGTAAACCGTACATTTAAGAACTGCAGTCAATTGAGTGGTATGACTTCGAACCTTGGATTGAGCTTAATAAGAAAAAAAACCTATTTGGGAGATTGAAATGTTTAATTCGGTGTTAAATTGGGATTGTGAAACAGGAAAAATGGGAGAAAGAGAGTTGAGTTGAAAATAATGGAAAAGGAATATAGAAGGAGTTACACACCAGGCTCAAAACCCTAACCATCAAATTCTAGCTTCCCAAACATGTTCCTTCAATCTAGGCATTGGGTTAGACTCGAGCTTGGTTGCCCCCGAATTAATGGGGCTAAGGCCCTCGATACCGGGTTACTAAAACATAGAGGGTAGCTTTTCCAAGATTCTGGATTTTTTTAAATTAGTGTATTTCAGATATGCTCAAATTGCCCCTATGTAGCCACAAACCAAAGAAATTCAGAGGATATTAGCACATGGAAATTTGCTGACATTCTACTTGGTGTTTTGTTTCAAACCGAATGAATAAGGACTAGGGAGCCTTTAGTTAGATATTTGCATCAATCTCTGTGGTGTGCACAAGGTTCATGGTAAAATAGAGATCTTAGGTGGTATTCTTAGATGACTAACAAGTTGAAATAAGTAGAGCTGCTTGGAAACAGTCTTCTTCAAGTCGAAATGTGAGGGTTGAGCAAACCCTCTCGTAGAAATTTTAATAGATTGCTCGAAAACTTGCGAGACATATTATGGTTGTTTATGTCATATAGAGCGAAAGGTCATGTGATGTTAATCGTAAACTTTTATTTTGGTTAATATCAAATGCTTGTAGCATATTTCTTCCAAATTGATTTCTACTTCTAATGAAATACACACTCCACATTGTGTGTACACATACGGCACCGCTTATCAGTTTGTATATCACGGCACACGTTGTATGCAGTTGTGTGAAACGCGGTGAACGCCCAATTGTAATTTCTCCATTTTCGTTTTTGGGTTGCAGACAACCTCAAAATTCCTCAAGGATGGCTCTGAGATTTGTGTTGTCCTTGGCAGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCTGCATCAGCTGTAGGACTACATCTAGTTGGGGTTAGTATTGTAACGTCTTTACTTCAAGCATAATCGTTCTGACTTTAATAACTTATCCGCTTGTATTGTCTCCTTCTCAGCCATTGGGATTTAAGGTTGATGACACAAAGCTAAAGCGGGCGGGTTTGGATTATTGGCCGTATCCCATGATGCATTTTATATATGATTCGTTTGCTCTCATTTACAGTTAAATATCAGGGATTCAATATTAGGAAATTGTGCTTTACTTTGTCTTGAACTGTTTGAGTTTTCTATTTCGTGCTGTCACTGTTCGTTCAATGTTTTTGGGTTTTAACATTGTATTTTAGATACGTTGTCGTTAAAATTCATAACACATGGGCGGATTTTCAAGAATACTTTAAGAAGCAGGTAAGCATGGACAAGATTCCATTTTAGTTTCCTCTTTGCATCCTACATTGTTGGAAACTATGTCTACCAACTCAATGATATGTACAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAAAGAGGAACAACACATCATTCGGTGAGTGCCTATTTCTCTGATGATTTGATTTCTATACGAGTCGTCGATTGCTCATAGGCCAGGAGGAATTTTGAGTAACAATTTGTTTTCTCATTTCATTTCTGCAGGATTTTTCGTACAAGCGGGGGGATTACTTGTTGTTCGGCTCAGAAACTACTGGATTACCACCTGAAGCGCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACATTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTGAGTGTTGGTATTGCTATATACGAAGCTTCAAGACAACTAAATTACGAGCAACTTCAGGTTTCATCAGAGAGTTGTACTAACAATGAACAATCTATTATTACAGAGGATGTTTTTGCTTGA

mRNA sequence

ATGCCGTTAATGGACACAACAACAGCCACTTTCAGAACTTTAAACCCATTTATCCCTTTTCAACTACGCTCATCCAGGTCTCTTCATCTTCTTCGCTCTCACTCCTTCTCCCTTTTCACCAACAAACACCACAATCCATCTTCTTCATCCTCCCGCCGGTTACTCCCCTTCTGCTCTTCATCTGACAGAGTGGAGGGTTCTGGTTCCCTACCGAGCGGTGTTGGTGAGGCCGTCAATGGGGTCTCGCATCCAAAGCTTCTTCAAGTTGTATTGGTGTCTCCCTTGACAACCTCAAAATTCCTCAAGGATGGCTCTGAGATTTGTGTTGTCCTTGGCAGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCTGCATCAGCTATACGTTGTCGTTAAAATTCATAACACATGGGCGGATTTTCAAGAATACTTTAAGAAGCAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAAAGAGGAACAACACATCATTCGGATTTTTCGTACAAGCGGGGGGATTACTTGTTGTTCGGCTCAGAAACTACTGGATTACCACCTGAAGCGCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACATTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTGAGTGTTGGTATTGCTATATACGAAGCTTCAAGACAACTAAATTACGAGCAACTTCAGGTTTCATCAGAGAGTTGTACTAACAATGAACAATCTATTATTACAGAGGATGTTTTTGCTTGA

Coding sequence (CDS)

ATGCCGTTAATGGACACAACAACAGCCACTTTCAGAACTTTAAACCCATTTATCCCTTTTCAACTACGCTCATCCAGGTCTCTTCATCTTCTTCGCTCTCACTCCTTCTCCCTTTTCACCAACAAACACCACAATCCATCTTCTTCATCCTCCCGCCGGTTACTCCCCTTCTGCTCTTCATCTGACAGAGTGGAGGGTTCTGGTTCCCTACCGAGCGGTGTTGGTGAGGCCGTCAATGGGGTCTCGCATCCAAAGCTTCTTCAAGTTGTATTGGTGTCTCCCTTGACAACCTCAAAATTCCTCAAGGATGGCTCTGAGATTTGTGTTGTCCTTGGCAGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCTGCATCAGCTATACGTTGTCGTTAAAATTCATAACACATGGGCGGATTTTCAAGAATACTTTAAGAAGCAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAAAGAGGAACAACACATCATTCGGATTTTTCGTACAAGCGGGGGGATTACTTGTTGTTCGGCTCAGAAACTACTGGATTACCACCTGAAGCGCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACATTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTGAGTGTTGGTATTGCTATATACGAAGCTTCAAGACAACTAAATTACGAGCAACTTCAGGTTTCATCAGAGAGTTGTACTAACAATGAACAATCTATTATTACAGAGGATGTTTTTGCTTGA

Protein sequence

MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSSSDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPLTTSKFLKDGSEICVVLGRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQVSSESCTNNEQSIITEDVFA*

Homology

[Close]

BLAST of Chy1G018800 vs. ExPASy Swiss-Prot
Match: P0AGJ8 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=trmL PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-12
Identity = 45/109 (41.28%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 127 HQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFGSETTGL 186
           H+   V + H+  A     F + E  +RL A T +GT  HS  SY+ GDYL+FG ET GL
Sbjct: 52  HEFTAVTRHHDYRA-----FLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGL 111

Query: 187 PPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 236
           P   L+    +      +RIPMV    R +NLS +V + +YEA RQL Y
Sbjct: 112 PASILDALPAEQ----KIRIPMVPD-SRSMNLSNAVSVVVYEAWRQLGY 150

[Close]

BLAST of Chy1G018800 vs. ExPASy Swiss-Prot
Match: P0AGJ7 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmL PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-12
Identity = 45/109 (41.28%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 127 HQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFGSETTGL 186
           H+   V + H+  A     F + E  +RL A T +GT  HS  SY+ GDYL+FG ET GL
Sbjct: 52  HEFTAVTRHHDYRA-----FLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGL 111

Query: 187 PPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 236
           P   L+    +      +RIPMV    R +NLS +V + +YEA RQL Y
Sbjct: 112 PASILDALPAEQ----KIRIPMVPD-SRSMNLSNAVSVVVYEAWRQLGY 150

[Close]

BLAST of Chy1G018800 vs. ExPASy Swiss-Prot
Match: P44868 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=trmL PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.8e-12
Identity = 43/107 (40.19%), Postives = 58/107 (54.21%), Query Frame = 0


Query: 130 YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFGSETTGLPPE 189
           +  +K H T+    E F + E  KRL A T +G   HS   +K GDYL+FG ET G+P  
Sbjct: 54  FAEIKRHKTF----EAFLESEKPKRLFALTTKGCPAHSQVKFKLGDYLMFGPETRGIPMS 113

Query: 190 ALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYE 237
            L +   +      +RIPM     R +NLS SV + +YEA RQL Y+
Sbjct: 114 ILNEMPMEQ----KIRIPMTAN-SRSMNLSNSVAVTVYEAWRQLGYK 151

[Close]

BLAST of Chy1G018800 vs. ExPASy Swiss-Prot
Match: C7BSD3 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) OX=553480 GN=trmL PE=3 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-11
Identity = 45/105 (42.86%), Postives = 60/105 (57.14%), Query Frame = 0


Query: 133 VKIHNTWADFQEYFKKQEGEK--RLLAFTKRGTTHHSDFSYKRGDYLLFGSETTGLPPEA 192
           +K H+ +  F E  +   GE   RL A T +GT  HS  SY+ GDYL+FG ET GLP   
Sbjct: 57  IKQHHDYHSFLEN-EGVTGESFARLFALTTKGTPAHSAVSYQAGDYLMFGPETRGLPSYV 116

Query: 193 LEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 236
           L++   Q      +RIPM+    R +NLS SV + ++EA RQL Y
Sbjct: 117 LDNMPPQQ----KIRIPMLAD-SRSMNLSNSVAVVVFEAWRQLGY 155

[Close]

BLAST of Chy1G018800 vs. ExPASy Swiss-Prot
Match: B8CHC9 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Shewanella piezotolerans (strain WP3 / JCM 13877) OX=225849 GN=trmL PE=3 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-11
Identity = 39/95 (41.05%), Postives = 60/95 (63.16%), Query Frame = 0


Query: 141 DFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFGSETTGLPPEALEDCKNQPFG 200
           +F+ + K  +G KR++A T +G+  HS+ ++++ D LLFG E+ GLP + ++    +   
Sbjct: 62  NFESFLKAMQG-KRIMACTTKGSRPHSELTFQQDDVLLFGPESRGLPADIIDSIPTEQ-- 121

Query: 201 GGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 236
              LRIPM+ T  R LNLS +V I  YEA RQL+Y
Sbjct: 122 --RLRIPMMST-SRSLNLSNAVAIISYEAWRQLDY 150

[Close]

BLAST of Chy1G018800 vs. ExPASy TrEMBL
Match: A0A5D3CFA4 (Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001000 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.0e-113
Identity = 221/268 (82.46%), Postives = 228/268 (85.07%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTTTAT RTLNPF PFQ RSSRS HLLR HSFSLFTNKHHNPSSSSSRRLLPFCSS
Sbjct: 49  MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSSRRLLPFCSS 108

Query: 61  SDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---------TTSKFLKDGSEICVVL 120
           S+RVEGSGSLPSGVGEAVNGVSHPK+LQVVLVSPL          T      G  +   L
Sbjct: 109 SERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 168

Query: 121 GRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180
           G  +         + +  YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY
Sbjct: 169 GFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 228

Query: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240
           KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
Sbjct: 229 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 288

Query: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 260
           QLNYEQLQVSSESCTN+EQSIITEDVFA
Sbjct: 289 QLNYEQLQVSSESCTNDEQSIITEDVFA 316

[Close]

BLAST of Chy1G018800 vs. ExPASy TrEMBL
Match: A0A1S3CFJ1 (putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499907 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.0e-113
Identity = 221/268 (82.46%), Postives = 228/268 (85.07%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTTTAT RTLNPF PFQ RSSRS HLLR HSFSLFTNKHHNPSSSSSRRLLPFCSS
Sbjct: 1   MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSSRRLLPFCSS 60

Query: 61  SDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---------TTSKFLKDGSEICVVL 120
           S+RVEGSGSLPSGVGEAVNGVSHPK+LQVVLVSPL          T      G  +   L
Sbjct: 61  SERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 121 GRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180
           G  +         + +  YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY
Sbjct: 121 GFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180

Query: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240
           KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
Sbjct: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240

Query: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 260
           QLNYEQLQVSSESCTN+EQSIITEDVFA
Sbjct: 241 QLNYEQLQVSSESCTNDEQSIITEDVFA 268

[Close]

BLAST of Chy1G018800 vs. ExPASy TrEMBL
Match: A0A0A0K6L9 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428850 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.6e-112
Identity = 221/268 (82.46%), Postives = 226/268 (84.33%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTTTAT R LNPF PFQLRSSRSLHLLR  SFSLFTNKHHN SSSS RRLLPFCSS
Sbjct: 1   MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 60

Query: 61  SDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---------TTSKFLKDGSEICVVL 120
           SDRVEGSGSLP+GVGEAVNGVSHPKLLQVVLVSPL          T      G  +   L
Sbjct: 61  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 121 GRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180
           G  +         + +  YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY
Sbjct: 121 GFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180

Query: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240
           KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
Sbjct: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240

Query: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 260
           QLNYEQLQVSSESCTNNEQSIITEDVFA
Sbjct: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 268

[Close]

BLAST of Chy1G018800 vs. ExPASy TrEMBL
Match: A0A6J1KVT5 (uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 5.2e-89
Identity = 187/270 (69.26%), Postives = 208/270 (77.04%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTT  T RTL+PF PF LRSS S  LLR+  FS F++KH +P SSSSRRLLPFCS 
Sbjct: 50  MPLMDTTATTLRTLHPFSPFLLRSSTSHRLLRARCFSHFSHKHRHPCSSSSRRLLPFCSF 109

Query: 61  SDRVEG-SGSLPSGVGEAVNGVSHPKLLQVVLVSPL---------TTSKFLKDGSEICVV 120
           S++ EG SGSLP GVGEA+NGVS  K+LQVVLVSPL          T      G  +   
Sbjct: 110 SEKGEGSSGSLPRGVGEALNGVSQQKVLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 169

Query: 121 LGRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFS 180
           LG  +         + +  YVVVKIHNTWA+F++YFKKQEGEKRLLAFTKRGTT HSDFS
Sbjct: 170 LGFKVDDTKLKRAGLDYWPYVVVKIHNTWAEFRDYFKKQEGEKRLLAFTKRGTTLHSDFS 229

Query: 181 YKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240
           YK+GDYLLF SET+GLP EALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS
Sbjct: 230 YKQGDYLLFRSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 289

Query: 241 RQLNYEQLQVSSESCT-NNEQSIITEDVFA 260
           RQLNY+QLQ+  E CT NNEQSIITED+FA
Sbjct: 290 RQLNYQQLQIEPEGCTNNNEQSIITEDLFA 319

[Close]

BLAST of Chy1G018800 vs. ExPASy TrEMBL
Match: A0A6J1H4X6 (uncharacterized protein LOC111460609 OS=Cucurbita moschata OX=3662 GN=LOC111460609 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 8.9e-89
Identity = 188/271 (69.37%), Postives = 213/271 (78.60%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTT  T RTL+PF PF LR+S S  LLR+  FS F++KH +P SSSSRRLLPFCS 
Sbjct: 1   MPLMDTTATTLRTLHPFSPFLLRASTSHRLLRARCFSHFSHKHRHPCSSSSRRLLPFCSF 60

Query: 61  SDRVEG-SGSLPSGVGEAVNGVSHPKLLQVVLVSPL---TTSKFLKDGSEICVVLGRFLG 120
           S++ EG SGSLP GVG+A+NGVS  K+LQVVLVSPL    T    +  +   V L   +G
Sbjct: 61  SEKGEGSSGSLPRGVGKALNGVSQQKVLQVVLVSPLIPGNTGSIARTCAASAVGL-HLVG 120

Query: 121 TL-VALPEHVLHQL------YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDF 180
            L   + +  L +       YVVVKIHNTWA+F++YFKKQEGEKRLLAFTKRGTT HSDF
Sbjct: 121 PLGFKVDDKKLKRAGLDYWPYVVVKIHNTWAEFRDYFKKQEGEKRLLAFTKRGTTLHSDF 180

Query: 181 SYKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA 240
           SYK+GDYLLFGSET+GLP EALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Sbjct: 181 SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA 240

Query: 241 SRQLNYEQLQVSSESCT-NNEQSIITEDVFA 260
           SRQLNY+QLQ+  E CT NNEQSIITED+FA
Sbjct: 241 SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA 270

[Close]

BLAST of Chy1G018800 vs. NCBI nr
Match: XP_008461270.1 (PREDICTED: putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo])

HSP 1 Score: 419 bits (1077), Expect = 5.64e-146
Identity = 221/268 (82.46%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTTTAT RTLNPF PFQ RSSRS HLLR HSFSLFTNKHHNPSSSSSRRLLPFCSS
Sbjct: 1   MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSSRRLLPFCSS 60

Query: 61  SDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---TTSKFLKD------GSEICVVL 120
           S+RVEGSGSLPSGVGEAVNGVSHPK+LQVVLVSPL    T    +       G  +   L
Sbjct: 61  SERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 121 GRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180
           G  +         + +  YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY
Sbjct: 121 GFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180

Query: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240
           KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
Sbjct: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240

Query: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 259
           QLNYEQLQVSSESCTN+EQSIITEDVFA
Sbjct: 241 QLNYEQLQVSSESCTNDEQSIITEDVFA 268

[Close]

BLAST of Chy1G018800 vs. NCBI nr
Match: KAA0058644.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >TYK10451.1 putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 419 bits (1077), Expect = 3.11e-145
Identity = 221/268 (82.46%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTTTAT RTLNPF PFQ RSSRS HLLR HSFSLFTNKHHNPSSSSSRRLLPFCSS
Sbjct: 49  MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSSRRLLPFCSS 108

Query: 61  SDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---TTSKFLKD------GSEICVVL 120
           S+RVEGSGSLPSGVGEAVNGVSHPK+LQVVLVSPL    T    +       G  +   L
Sbjct: 109 SERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 168

Query: 121 GRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180
           G  +         + +  YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY
Sbjct: 169 GFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 228

Query: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240
           KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
Sbjct: 229 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 288

Query: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 259
           QLNYEQLQVSSESCTN+EQSIITEDVFA
Sbjct: 289 QLNYEQLQVSSESCTNDEQSIITEDVFA 316

[Close]

BLAST of Chy1G018800 vs. NCBI nr
Match: XP_004135918.1 (uncharacterized protein LOC101203702 [Cucumis sativus] >KGN45148.1 hypothetical protein Csa_016173 [Cucumis sativus])

HSP 1 Score: 416 bits (1069), Expect = 9.33e-145
Identity = 221/268 (82.46%), Postives = 227/268 (84.70%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSSRRLLPFCSS 60
           MPLMDTTTAT R LNPF PFQLRSSRSLHLLR  SFSLFTNKHHN SSSS RRLLPFCSS
Sbjct: 1   MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 60

Query: 61  SDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---TTSKFLKD------GSEICVVL 120
           SDRVEGSGSLP+GVGEAVNGVSHPKLLQVVLVSPL    T    +       G  +   L
Sbjct: 61  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 121 GRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180
           G  +         + +  YVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY
Sbjct: 121 GFKVDDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSY 180

Query: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240
           KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
Sbjct: 181 KRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR 240

Query: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 259
           QLNYEQLQVSSESCTNNEQSIITEDVFA
Sbjct: 241 QLNYEQLQVSSESCTNNEQSIITEDVFA 268

[Close]

BLAST of Chy1G018800 vs. NCBI nr
Match: XP_038898778.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 373 bits (957), Expect = 1.12e-127
Identity = 205/270 (75.93%), Postives = 216/270 (80.00%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSS-RRLLPFCS 60
           MPLMDT T T RTLNPF PFQLRSSR  HLLR H FS FTNK HNPSSSSS  RLLPFCS
Sbjct: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60

Query: 61  SSDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---TTSKFLKD------GSEICVV 120
            S+RVEGSGSLPSGVGEAVNGVS PKLLQVVLVSPL    T    +       G  +   
Sbjct: 61  LSERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120

Query: 121 LGRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFS 180
           LG  +         + +  YVVVKIH+TWADF++YF KQEGEKRLLAFTKRGTT HSDFS
Sbjct: 121 LGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKRGTTLHSDFS 180

Query: 181 YKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240
           YKRGDYLLFGSET+GLP EALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS
Sbjct: 181 YKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEAS 240

Query: 241 RQLNYEQLQ-VSSESCTNNEQSIITEDVFA 259
           RQLNYEQLQ V SESCT NEQSIITED+FA
Sbjct: 241 RQLNYEQLQQVGSESCTGNEQSIITEDLFA 270

[Close]

BLAST of Chy1G018800 vs. NCBI nr
Match: XP_038898777.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 365 bits (937), Expect = 1.70e-124
Identity = 204/279 (73.12%), Postives = 217/279 (77.78%), Query Frame = 0


Query: 1   MPLMDTTTATFRTLNPFIPFQLRSSRSLHLLRSHSFSLFTNKHHNPSSSSS-RRLLPFCS 60
           MPLMDT T T RTLNPF PFQLRSSR  HLLR H FS FTNK HNPSSSSS  RLLPFCS
Sbjct: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60

Query: 61  ---------SSDRVEGSGSLPSGVGEAVNGVSHPKLLQVVLVSPL---TTSKFLKD---- 120
                    +++RVEGSGSLPSGVGEAVNGVS PKLLQVVLVSPL    T    +     
Sbjct: 61  LFWFCCVNGAAERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAAS 120

Query: 121 --GSEICVVLGRFLGTLVALPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKR 180
             G  +   LG  +         + +  YVVVKIH+TWADF++YF KQEGEKRLLAFTKR
Sbjct: 121 AVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHHTWADFRDYFMKQEGEKRLLAFTKR 180

Query: 181 GTTHHSDFSYKRGDYLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVS 240
           GTT HSDFSYKRGDYLLFGSET+GLP EALEDCKNQPFGGGTLRIPMVETYVRCLNLSVS
Sbjct: 181 GTTLHSDFSYKRGDYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVS 240

Query: 241 VGIAIYEASRQLNYEQLQ-VSSESCTNNEQSIITEDVFA 259
           VGIAIYEASRQLNYEQLQ V SESCT NEQSIITED+FA
Sbjct: 241 VGIAIYEASRQLNYEQLQQVGSESCTGNEQSIITEDLFA 279

[Close]

BLAST of Chy1G018800 vs. TAIR 10
Match: AT5G26880.2 (AGAMOUS-like 26 )

HSP 1 Score: 224.9 bits (572), Expect = 7.3e-59
Identity = 115/199 (57.79%), Postives = 141/199 (70.85%), Query Frame = 0


Query: 70  LPSGVGEAVNGVSHPKLLQVVLVSP---------LTTSKFLKDGSEICVVLGRFLGTLVA 129
           LP G GE V   +  KLL VVLVSP           T      G  +   LG  +     
Sbjct: 65  LPRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARV 124

Query: 130 LPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFG 189
               + +  +VVVK H++WA+FQEYF+ QEGEKR++AFTKRGT  HSDFSY+ GDYLLFG
Sbjct: 125 KRAGLDYWPFVVVKAHSSWAEFQEYFRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFG 184

Query: 190 SETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQV 249
           SET+GLPPEAL DC ++P+GGGTLRIPMVETYVRCLNLSVSVGIA+YEASRQLNYEQ++ 
Sbjct: 185 SETSGLPPEALSDCNHEPYGGGTLRIPMVETYVRCLNLSVSVGIALYEASRQLNYEQIEC 244

Query: 250 SSESCTNNEQSIITEDVFA 260
           + + C N E+ ++TED+FA
Sbjct: 245 APQGCVNGEEPLLTEDIFA 263

[Close]

BLAST of Chy1G018800 vs. TAIR 10
Match: AT5G26880.1 (AGAMOUS-like 26 )

HSP 1 Score: 172.2 bits (435), Expect = 5.6e-43
Identity = 90/160 (56.25%), Postives = 108/160 (67.50%), Query Frame = 0


Query: 70  LPSGVGEAVNGVSHPKLLQVVLVSP---------LTTSKFLKDGSEICVVLGRFLGTLVA 129
           LP G GE V   +  KLL VVLVSP           T      G  +   LG  +     
Sbjct: 65  LPRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARV 124

Query: 130 LPEHVLHQLYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGDYLLFG 189
               + +  +VVVK H++WA+FQEYF+ QEGEKR++AFTKRGT  HSDFSY+ GDYLLFG
Sbjct: 125 KRAGLDYWPFVVVKAHSSWAEFQEYFRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFG 184

Query: 190 SETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSV 221
           SET+GLPPEAL DC ++P+GGGTLRIPMVETYVRCLNLS+
Sbjct: 185 SETSGLPPEALSDCNHEPYGGGTLRIPMVETYVRCLNLSI 224

The following BLAST results are available for this feature:

BLAST of Chy1G018800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0AGJ8	2.9e-12	41.28	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli O6:H1 (strain CFT...	[more]
P0AGJ7	2.9e-12	41.28	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=8...	[more]
P44868	3.8e-12	40.19	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Haemophilus influenzae (strain ATC...	[more]
C7BSD3	1.9e-11	42.86	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Photorhabdus asymbiotica subsp. as...	[more]
B8CHC9	3.2e-11	41.05	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Shewanella piezotolerans (strain W...	[more]

BLAST of Chy1G018800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFA4	3.0e-113	82.46	Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa ...	[more]
A0A1S3CFJ1	3.0e-113	82.46	putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=L...	[more]
A0A0A0K6L9	2.6e-112	82.46	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428...	[more]
A0A6J1KVT5	5.2e-89	69.26	uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049...	[more]
A0A6J1H4X6	8.9e-89	69.37	uncharacterized protein LOC111460609 OS=Cucurbita moschata OX=3662 GN=LOC1114606...	[more]

BLAST of Chy1G018800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008461270.1	5.64e-146	82.46	PREDICTED: putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo]	[more]
KAA0058644.1	3.11e-145	82.46	putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >...	[more]
XP_004135918.1	9.33e-145	82.46	uncharacterized protein LOC101203702 [Cucumis sativus] >KGN45148.1 hypothetical ...	[more]
XP_038898778.1	1.12e-127	75.93	putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X2 [Benincasa hispid...	[more]
XP_038898777.1	1.70e-124	73.12	putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X1 [Benincasa hispid...	[more]

BLAST of Chy1G018800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26880.2	7.3e-59	57.79	AGAMOUS-like 26	[more]
AT5G26880.1	5.6e-43	56.25	AGAMOUS-like 26	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 87..241 e-value: 6.6E-23 score: 83.4
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 130..227 e-value: 7.1E-15 score: 55.4
None	No IPR available	PIRSR	PIRSR029256-1	PIRSR029256-1	coord: 134..235 e-value: 3.3E-18 score: 63.5
IPR016914	tRNA (cytidine/uridine-2'-O-)-methyltransferase	PANTHER	PTHR42971	TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASE	coord: 130..258
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 130..239

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G018800.1	Chy1G018800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002131	wobble position cytosine ribose methylation
biological_process	GO:0002132	wobble position uridine ribose methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity