Chy1G016290 (gene) Cucumber (hystrix) v1

Overview
NameChy1G016290
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionisoamylase 2, chloroplastic
LocationchrH01: 22239020 .. 22241677 (-)
RNA-Seq ExpressionChy1G016290
SyntenyChy1G016290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACTTTCCTGTCTTCACTTGTCATACAGCCCTTCTGTGCATATAATTGTGGAGTTACTGAATCACTAAAACTTGCTGTAAGTGATCACCTTACATATGGACAGAAGACAAAGTATCAGTTTGGAAAAATGGATGAGGCTAGAATGCTAGCTCATGGAGAAAGTAAAGTAGGTGCTGTAAAATCTTCGCACCGCAATCTTAGTAAAACATATGCTAAATCGGGAATTTCGGTTGGGAAAAGTGGGCAGAGATTGGGCATTGGTGGTAAAAGTAAGGAACAAAGAAAGGTTGCAACCTATCTATTTAGAACAGAGTTTGGGGATTTGGTGAATGTTTTTGTTGGAAAGAAAGGGTCTACTTTTACAGTTAATATTGAGATTTCTTCCATGCAACTAGTGAGCATTGGAGAAGCACTACTGCTTAGTTGGGGGGTTTATAGATCTGATTCAGCTCTTGTAACCCCCAACTTTCAGAGTTCACCCCCAGATGAGACAGCTGAAGCCACTGAAACCCCTTTTGTAAAAACTTCTGAAGGAAAATTTTCTGTTGAATTGGAGTTTGATGCTAAGCACACTCCTTTCTACCTTTCTTTTGTGTTGAAATATCCAATGGGTGTTGATTCAGGCAGCTCCAAAATTAGAAGTCATAGGAAGACTAGCTTTTCTGTACCTGTTGGGTTTGGTCGAGGTTATCCGAGTCCGCTGGGTCTTTCCATTTCTGGTGGCGGTTCTATAAATTTTTCCATTTTCTCAAGCACTACAGAAAGTTTAGTTTTATGTCTGTACAATGATAGCACATCAGAAAAACCTCTGTTAGAGCTAGATTTAGATCCATATATTAACCGATCAGGTAATATTTGGCATGCCTCATTTGAAGGTGCCTCAAAATTTGTAAGCTATGGTTATCAATGCAAGGGGTCAAAGTCTCATGAAAACCAAGATGGACTCAAAGTAAGTCGGATTGTCGTGGATCCGTATGCTAAGATCCTTGCACCATCTATTCCTAAATCCAGTGGTCAGGGACTTAGATTGCCTTCAAAATTTCTTGGGCAAATATCCAAAGTTCCCACTTTTGATTGGGATGGTGAAGTCCGTCCTAACTTGCCAATGGAAAAACTTTTTGTTTATCGGTTAAATGTGGAGCGTTTTACCATGGACAAATCAAGTCAGCTTCCAGCAGATATTGCCGGTACATTTTCTGGTTTGACCAAGAAGTTGTTGCATTTTAAAAACTTAGGCGTGAATGCAGTTCTACTAGAGCCAATCTTTCAATTTGATGAGAAAGAAGGACCATATTTTCCCTTTCACTTCTTTTCACCGACAAACAATTATGGTCCTTCTGGGGCTTCAATTTCTGCTATAAACTCCATGAAGGAAATGGTTAAAGAACTGCATGCCAATGGATTAGAGGTCATCTTGGAAGTTGTTTATACTCACACTTCCGTGGATGGAGCACTGCAAGGCATTGATGACTCATCTTACTATTTTACCAATAGGGTTGCAAATTTGGAAGAAAAAAGTGCTCTGAACTGCAATTATCCCATGGTGCAGCAATTACTTTTGGATAGTCTTCGATATTGGGTGACTGAGTTTCATGTAGATGGTTTTTGTTTCATAAATGCTTCATTTTTGCTGCGAGGACATCATGGTGAATTGTTGTTTCGCCCTCCTTTTGTAGAAGCAATTGCTTTTGATCCGCTTTTATCAAAAACTAAACTAGTTGCAGACTTCTGGGATCCACAGGAGTTGATATCAAAGGAAACTCGTTTCCCTCATTGGAAGAGATGGGCAGAGGTGAATTCAAAGTTCTGTAGTGATATAAGAGACTTTTTCAGGGGAGAAGGTCTTATTAGTAGCCTTGCAACCCGGCTTTGTGGGAGTGGAGATGTATTCTCAGATGGTCGAGGCCCTGCATTCTCTTTCAATTTGATTGCTAGAAACGTTGGCCTTCCTCTTGTCGACCTTGTGAGCTTTAGCAACAGCAATTTAGCTTCAGAGTTGAGTTGGAACTGTGGGGAAGAAGGACCGACAAGTAATCTTAAAGTTCTAGAGAAACGACTTAAACAAATTCGTAATTTTATATTTGTGTTGTTTGTTTCACTTGGTGTTCCTGTTCTTAACATGGGAGACGAGTGTGGCCAATCTTCAGGGGGATCTGTGGCATTCAATGATAGGAGGTCATTCAATTGGGATTTACTAAAAACGGATTTCGGTACTCAAACCACACAATTCATCACATTTTTGAGTTCATTTAGATCAAGGCGTTTTGACCTTTTCCAGAGCAGAAACTTTCTGAAGGGAGAGAACATAGACTGGTTTGATAATAACCAATCACCACCACAATGGGAAGATGCTTCTTGCAAATTCCTGGCTGTGATGCTGAGGGCTGATAAAGAGGACAATGAATCAATCACCGAGAAACCCAAGACTCGAAGCGACATATTCATGGTGTTCAATGCATCTGACCAATCTGAAAGTGTTGCTTTACCCGAACCATTAGAAGGTACATCCTGGTTTAGGGTGGTTGACACAGCTCTCCCTTTTCCTGGGTTTTTCTCATCCGATGGTGAGCTGGTGCCAATGACAGGATCTGTTACTTACGAAATACAAGCTCACAGTTGTGCACTTTTCGAGGCTAAAAGTGCCAATGGTTGA

mRNA sequence

ATGGCAACTTTCCTGTCTTCACTTGTCATACAGCCCTTCTGTGCATATAATTGTGGAGTTACTGAATCACTAAAACTTGCTGTAAGTGATCACCTTACATATGGACAGAAGACAAAGTATCAGTTTGGAAAAATGGATGAGGCTAGAATGCTAGCTCATGGAGAAAGTAAAGTAGGTGCTGTAAAATCTTCGCACCGCAATCTTAGTAAAACATATGCTAAATCGGGAATTTCGGTTGGGAAAAGTGGGCAGAGATTGGGCATTGGTGGTAAAAGTAAGGAACAAAGAAAGGTTGCAACCTATCTATTTAGAACAGAGTTTGGGGATTTGGTGAATGTTTTTGTTGGAAAGAAAGGGTCTACTTTTACAGTTAATATTGAGATTTCTTCCATGCAACTAGTGAGCATTGGAGAAGCACTACTGCTTAGTTGGGGGGTTTATAGATCTGATTCAGCTCTTGTAACCCCCAACTTTCAGAGTTCACCCCCAGATGAGACAGCTGAAGCCACTGAAACCCCTTTTGTAAAAACTTCTGAAGGAAAATTTTCTGTTGAATTGGAGTTTGATGCTAAGCACACTCCTTTCTACCTTTCTTTTGTGTTGAAATATCCAATGGGTGTTGATTCAGGCAGCTCCAAAATTAGAAGTCATAGGAAGACTAGCTTTTCTGTACCTGTTGGGTTTGGTCGAGGTTATCCGAGTCCGCTGGGTCTTTCCATTTCTGGTGGCGGTTCTATAAATTTTTCCATTTTCTCAAGCACTACAGAAAGTTTAGTTTTATGTCTGTACAATGATAGCACATCAGAAAAACCTCTGTTAGAGCTAGATTTAGATCCATATATTAACCGATCAGGTAATATTTGGCATGCCTCATTTGAAGGTGCCTCAAAATTTGTAAGCTATGGTTATCAATGCAAGGGGTCAAAGTCTCATGAAAACCAAGATGGACTCAAAGTAAGTCGGATTGTCGTGGATCCGTATGCTAAGATCCTTGCACCATCTATTCCTAAATCCAGTGGTCAGGGACTTAGATTGCCTTCAAAATTTCTTGGGCAAATATCCAAAGTTCCCACTTTTGATTGGGATGGTGAAGTCCGTCCTAACTTGCCAATGGAAAAACTTTTTGTTTATCGGTTAAATGTGGAGCGTTTTACCATGGACAAATCAAGTCAGCTTCCAGCAGATATTGCCGGTACATTTTCTGGTTTGACCAAGAAGTTGTTGCATTTTAAAAACTTAGGCGTGAATGCAGTTCTACTAGAGCCAATCTTTCAATTTGATGAGAAAGAAGGACCATATTTTCCCTTTCACTTCTTTTCACCGACAAACAATTATGGTCCTTCTGGGGCTTCAATTTCTGCTATAAACTCCATGAAGGAAATGGTTAAAGAACTGCATGCCAATGGATTAGAGGTCATCTTGGAAGTTGTTTATACTCACACTTCCGTGGATGGAGCACTGCAAGGCATTGATGACTCATCTTACTATTTTACCAATAGGGTTGCAAATTTGGAAGAAAAAAGTGCTCTGAACTGCAATTATCCCATGGTGCAGCAATTACTTTTGGATAGTCTTCGATATTGGGTGACTGAGTTTCATGTAGATGGTTTTTGTTTCATAAATGCTTCATTTTTGCTGCGAGGACATCATGGTGAATTGTTGTTTCGCCCTCCTTTTGTAGAAGCAATTGCTTTTGATCCGCTTTTATCAAAAACTAAACTAGTTGCAGACTTCTGGGATCCACAGGAGTTGATATCAAAGGAAACTCGTTTCCCTCATTGGAAGAGATGGGCAGAGGTGAATTCAAAGTTCTGTAGTGATATAAGAGACTTTTTCAGGGGAGAAGGTCTTATTAGTAGCCTTGCAACCCGGCTTTGTGGGAGTGGAGATGTATTCTCAGATGGTCGAGGCCCTGCATTCTCTTTCAATTTGATTGCTAGAAACGTTGGCCTTCCTCTTGTCGACCTTGTGAGCTTTAGCAACAGCAATTTAGCTTCAGAGTTGAGTTGGAACTGTGGGGAAGAAGGACCGACAAGTAATCTTAAAGTTCTAGAGAAACGACTTAAACAAATTCGTAATTTTATATTTGTGTTGTTTGTTTCACTTGGTGTTCCTGTTCTTAACATGGGAGACGAGTGTGGCCAATCTTCAGGGGGATCTGTGGCATTCAATGATAGGAGGTCATTCAATTGGGATTTACTAAAAACGGATTTCGGTACTCAAACCACACAATTCATCACATTTTTGAGTTCATTTAGATCAAGGCGTTTTGACCTTTTCCAGAGCAGAAACTTTCTGAAGGGAGAGAACATAGACTGGTTTGATAATAACCAATCACCACCACAATGGGAAGATGCTTCTTGCAAATTCCTGGCTGTGATGCTGAGGGCTGATAAAGAGGACAATGAATCAATCACCGAGAAACCCAAGACTCGAAGCGACATATTCATGGTGTTCAATGCATCTGACCAATCTGAAAGTGTTGCTTTACCCGAACCATTAGAAGGTACATCCTGGTTTAGGGTGGTTGACACAGCTCTCCCTTTTCCTGGGTTTTTCTCATCCGATGGTGAGCTGGTGCCAATGACAGGATCTGTTACTTACGAAATACAAGCTCACAGTTGTGCACTTTTCGAGGCTAAAAGTGCCAATGGTTGA

Coding sequence (CDS)

ATGGCAACTTTCCTGTCTTCACTTGTCATACAGCCCTTCTGTGCATATAATTGTGGAGTTACTGAATCACTAAAACTTGCTGTAAGTGATCACCTTACATATGGACAGAAGACAAAGTATCAGTTTGGAAAAATGGATGAGGCTAGAATGCTAGCTCATGGAGAAAGTAAAGTAGGTGCTGTAAAATCTTCGCACCGCAATCTTAGTAAAACATATGCTAAATCGGGAATTTCGGTTGGGAAAAGTGGGCAGAGATTGGGCATTGGTGGTAAAAGTAAGGAACAAAGAAAGGTTGCAACCTATCTATTTAGAACAGAGTTTGGGGATTTGGTGAATGTTTTTGTTGGAAAGAAAGGGTCTACTTTTACAGTTAATATTGAGATTTCTTCCATGCAACTAGTGAGCATTGGAGAAGCACTACTGCTTAGTTGGGGGGTTTATAGATCTGATTCAGCTCTTGTAACCCCCAACTTTCAGAGTTCACCCCCAGATGAGACAGCTGAAGCCACTGAAACCCCTTTTGTAAAAACTTCTGAAGGAAAATTTTCTGTTGAATTGGAGTTTGATGCTAAGCACACTCCTTTCTACCTTTCTTTTGTGTTGAAATATCCAATGGGTGTTGATTCAGGCAGCTCCAAAATTAGAAGTCATAGGAAGACTAGCTTTTCTGTACCTGTTGGGTTTGGTCGAGGTTATCCGAGTCCGCTGGGTCTTTCCATTTCTGGTGGCGGTTCTATAAATTTTTCCATTTTCTCAAGCACTACAGAAAGTTTAGTTTTATGTCTGTACAATGATAGCACATCAGAAAAACCTCTGTTAGAGCTAGATTTAGATCCATATATTAACCGATCAGGTAATATTTGGCATGCCTCATTTGAAGGTGCCTCAAAATTTGTAAGCTATGGTTATCAATGCAAGGGGTCAAAGTCTCATGAAAACCAAGATGGACTCAAAGTAAGTCGGATTGTCGTGGATCCGTATGCTAAGATCCTTGCACCATCTATTCCTAAATCCAGTGGTCAGGGACTTAGATTGCCTTCAAAATTTCTTGGGCAAATATCCAAAGTTCCCACTTTTGATTGGGATGGTGAAGTCCGTCCTAACTTGCCAATGGAAAAACTTTTTGTTTATCGGTTAAATGTGGAGCGTTTTACCATGGACAAATCAAGTCAGCTTCCAGCAGATATTGCCGGTACATTTTCTGGTTTGACCAAGAAGTTGTTGCATTTTAAAAACTTAGGCGTGAATGCAGTTCTACTAGAGCCAATCTTTCAATTTGATGAGAAAGAAGGACCATATTTTCCCTTTCACTTCTTTTCACCGACAAACAATTATGGTCCTTCTGGGGCTTCAATTTCTGCTATAAACTCCATGAAGGAAATGGTTAAAGAACTGCATGCCAATGGATTAGAGGTCATCTTGGAAGTTGTTTATACTCACACTTCCGTGGATGGAGCACTGCAAGGCATTGATGACTCATCTTACTATTTTACCAATAGGGTTGCAAATTTGGAAGAAAAAAGTGCTCTGAACTGCAATTATCCCATGGTGCAGCAATTACTTTTGGATAGTCTTCGATATTGGGTGACTGAGTTTCATGTAGATGGTTTTTGTTTCATAAATGCTTCATTTTTGCTGCGAGGACATCATGGTGAATTGTTGTTTCGCCCTCCTTTTGTAGAAGCAATTGCTTTTGATCCGCTTTTATCAAAAACTAAACTAGTTGCAGACTTCTGGGATCCACAGGAGTTGATATCAAAGGAAACTCGTTTCCCTCATTGGAAGAGATGGGCAGAGGTGAATTCAAAGTTCTGTAGTGATATAAGAGACTTTTTCAGGGGAGAAGGTCTTATTAGTAGCCTTGCAACCCGGCTTTGTGGGAGTGGAGATGTATTCTCAGATGGTCGAGGCCCTGCATTCTCTTTCAATTTGATTGCTAGAAACGTTGGCCTTCCTCTTGTCGACCTTGTGAGCTTTAGCAACAGCAATTTAGCTTCAGAGTTGAGTTGGAACTGTGGGGAAGAAGGACCGACAAGTAATCTTAAAGTTCTAGAGAAACGACTTAAACAAATTCGTAATTTTATATTTGTGTTGTTTGTTTCACTTGGTGTTCCTGTTCTTAACATGGGAGACGAGTGTGGCCAATCTTCAGGGGGATCTGTGGCATTCAATGATAGGAGGTCATTCAATTGGGATTTACTAAAAACGGATTTCGGTACTCAAACCACACAATTCATCACATTTTTGAGTTCATTTAGATCAAGGCGTTTTGACCTTTTCCAGAGCAGAAACTTTCTGAAGGGAGAGAACATAGACTGGTTTGATAATAACCAATCACCACCACAATGGGAAGATGCTTCTTGCAAATTCCTGGCTGTGATGCTGAGGGCTGATAAAGAGGACAATGAATCAATCACCGAGAAACCCAAGACTCGAAGCGACATATTCATGGTGTTCAATGCATCTGACCAATCTGAAAGTGTTGCTTTACCCGAACCATTAGAAGGTACATCCTGGTTTAGGGTGGTTGACACAGCTCTCCCTTTTCCTGGGTTTTTCTCATCCGATGGTGAGCTGGTGCCAATGACAGGATCTGTTACTTACGAAATACAAGCTCACAGTTGTGCACTTTTCGAGGCTAAAAGTGCCAATGGTTGA

Protein sequence

MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGAVKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGSTFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSISGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTHTSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWFRVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSANG*
Homology
[Close]
BLAST of Chy1G016290 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 929.5 bits (2401), Expect = 2.9e-269
Identity = 471/882 (53.40%), Postives = 625/882 (70.86%), Query Frame = 0 

Query: 14  CAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGAVKSSHRNLSKTYA 73
           C  N G+T + K   S  L  G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKFP-SARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRKVATYLFRTEFGDLVNVFVGKKGSTFTVNIEI 133
            S I   K+   L     S+     + +KV +Y FRT+ G LV V V KK   +++ + +
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 SSMQLVSIGEA-LLLSWGVYRSDSALVTP-NFQSSPPDETAEATETPFVKTSEGKFSVEL 193
           SS++L    ++ L++ WGVYRSDS+   P +F++S  D     TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSIS-GGGS 253
           EFD K +PFYLSF LK   G D    ++ +HR T F +PVGF  G+P PLGLS      S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 INFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS ++ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFDWDGEV 373
           K +   +    ++   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 RPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTHTSVDG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+  G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCFINAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFC 613
           LRG HGE L RPP VEAIAFDPLL++TKL+AD WDP E++ KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 RRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPPQWED 793
           R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEDNESIT----EKPKTRSDIFMVFNASDQSESVALPEPLEGTSWFR 853
            + KFLA+ ++++ E+ E+ +     +PK+ +D+F+ FNASD  ESV LP   +G+ W R
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKS-NDLFIGFNASDHPESVVLPSLPDGSKWRR 852

Query: 854 VVDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           +VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 LVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

[Close]
BLAST of Chy1G016290 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 4.8e-216
Identity = 369/716 (51.54%), Postives = 501/716 (69.97%), Query Frame = 0 

Query: 183 SVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSISG 242
           + EL FD    PFYLSF+L      D+  ++IR+HRKTSF VPVG G G P+PLG+SISG
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSISG 164

Query: 243 GGSINFSIFSSTTESLVLCLY----NDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 302
            G++NF+++S    ++ L LY         ++P LE+DLDPYI+R+GN+WH S      +
Sbjct: 165 DGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVDGY 224

Query: 303 VSYGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSS--GQGLRLPS-KFLGQISK 362
           VSY + C G           + R ++DPYAK++   +  +S   +G+  PS +    ++ 
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284

Query: 363 VPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGV 422
            P+++W  +  P LP+EKL VYR NV  FT D+SS LP D AGTF+GL+ K+ HF++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344

Query: 423 NAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILE 482
           NA+LLEP+F F + +GPYFP+HFFSP N Y   G S+SAI SMK+MV+ +H NG+EV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404

Query: 483 VVYTHTSVDG------ALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWV 542
           VV+THT+         +++GID+SSYY  N +A   + S LNCN+P+ Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGC-KASILNCNHPVTQKLILDSLRHWV 464

Query: 543 TEFHVDGFCFINASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKET 602
            +FHVDGFCFINA FL+RG  GE L RPP +EAI FDP+LS TK++AD W P ++ + + 
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524

Query: 603 RFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNV 662
            FPHWKRWAEVN++F  D+R F + E LIS LATRLCGSGD+FS  RGPAFSFN ++RN 
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584

Query: 663 GLPLVDLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNM 722
           GL LVDLVSFSN +L SE SWNCGEEGP+ N  VL+ RL+QIRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644

Query: 723 GDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGE 782
           GDECG S+ GSV++ DR   NW  +KT F  + T FI+FL++ RSRR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704

Query: 783 NIDWFDNNQSPPQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVA 842
           NI W+ ++   P W+D +  FL + + A+ ++  +       R D+++ FNA+++S S A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVSAA 764

Query: 843 LPEPLEGTSWFRVVDTALPFPGFFS--SDGELVPMTGSVTYEIQAHSCALFEAKSA 884
           LP   EG+ W R+VDT+L FPGFF+  S+ ++  + G  +Y ++AH+C LFE+KSA
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798

[Close]
BLAST of Chy1G016290 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 3.3e-100
Identity = 244/701 (34.81%), Postives = 357/701 (50.93%), Query Frame = 0 

Query: 231 GYPSPLGLSISGGGSINFSIFSSTTESLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S    G INF++FS    S+ LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVSQVDKG-INFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLR 350
           + WH   E      V YGY+  G    +       S +++DPYAK++      S G   +
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 LPSKFLGQIS-KVPTFDWDGEVR-PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
             ++F G    +   FDW  + + PN+P + L +Y +NV  FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
             +K+ H ++LG+NAV L P+F+FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 SGASISAINSMKEMVKELHANGLEVILEVVYTHTS-------VDGALQGIDDSSYYF--- 530
            G  I A    KEMVK LH+ G+EVIL+VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 TNRVANLEE-KSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFLLRGHHGELLFR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R   G  L  
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNLIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    N +  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRR-FDLFQSRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + +  NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWFR 882
            W+++  KFLA  L      ++ I  +     DI++ FNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

[Close]
BLAST of Chy1G016290 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 2.8e-99
Identity = 252/714 (35.29%), Postives = 361/714 (50.56%), Query Frame = 0 

Query: 224 VPVGFGRGYPSPLGLSISGGGSINFSIFSSTTESLVLCLY-----NDSTSEKPLLELDLD 283
           +P  +  G   PLG+S   GG +NF++FS    S++LCL       +      ++E  LD
Sbjct: 96  MPFKYSSGKAFPLGVSQVEGG-LNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLD 155

Query: 284 PYINRSGNIWHASFEG-ASKFVSYGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPK 343
              N++G+IWH   EG  +  V YGY+  G +  +       S +++DPYAK++  S  K
Sbjct: 156 QQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLV--SGRK 215

Query: 344 SSGQGLRLPSKFLGQIS-KVPTFDWDGEVR-PNLPMEKLFVYRLNVERFTMDKSSQLPAD 403
             G      S+  G        FDW  + R PNLP   L +Y +NV  FT D+SS L + 
Sbjct: 216 YFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDST 275

Query: 404 IAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFP--------------FHFFSP 463
             G++ GL  K+ H   LGVNAV L P+F++DE E   +P               +FF+P
Sbjct: 276 SRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAP 335

Query: 464 TNNYGPSGAS-ISAINSMKEMVKELHANGLEVILEVVYTHTS-VDGA------LQGIDDS 523
            + Y  +G   ++A   +K+MVKELH  G+EVIL+VVY HT+  D A       +GID+ 
Sbjct: 336 MSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNK 395

Query: 524 SYYFTNRVANLEE------KSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFLLR 583
            YY  +   N E        + LNCN+P+V++L+LDSLR+WV E+H+DGF F  AS L R
Sbjct: 396 VYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCR 455

Query: 584 GHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFCSD 643
           G  G  L  PP ++ IA D +LS+ K++A+ WD   L     RFP+W RWAE N K+  D
Sbjct: 456 GPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLY-LVGRFPNWDRWAEWNGKYRDD 515

Query: 644 IRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNLIARNVGLPLVDLVSFSNSN-- 703
           +R F +G+ G+    ATR+ GS D++  + R P    N +  + G  L DLVS++  +  
Sbjct: 516 LRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHND 575

Query: 704 ---------LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 763
                         SWNCG EG T++L VL  R +Q++NF   L +S G P++ MGDE G
Sbjct: 576 ANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYG 635

Query: 764 -------QSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLK 823
                   S G     N   +F W+ L+        +F + +  FR     L + R FL 
Sbjct: 636 HTRYGNNNSYGHDTCIN---NFQWEQLEQRRDGH-FRFFSEMIKFRHSNPILRRDR-FLN 695

Query: 824 GENIDWFDNNQSPPQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSES 882
             ++ W ++      WE+   KFLA  +     D+ S         DI++ FNA D    
Sbjct: 696 KNDVTWHED-----CWENQESKFLAFTV----HDHNS-------GGDIYLAFNAHDYFVD 755

[Close]
BLAST of Chy1G016290 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.0e-93
Identity = 230/689 (33.38%), Postives = 338/689 (49.06%), Query Frame = 0 

Query: 231 GYPSPLGLSISGGGSINFSIFSSTTESLVLCLY--NDSTSEKPLLELDLDPYINRSGNIW 290
           G P+PLG +   GG +NF+++S+   +  LCL+  +D  +++   E+ LDP  NR+GN+W
Sbjct: 99  GMPAPLGATALDGG-VNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLKVSRIVVDPYAK-ILAPSIPKSSGQGLRLPS 350
           H   EG    + YGY+  G  +        VS +VVDPYAK +++       G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 351 KFLGQIS-KVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 410
           +  G I     TFDW G++    P + L +Y +++  FT   SS +  +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278

Query: 411 LLHFKNLGVNAVLLEPIFQFDEKE----------GPYFPFHFFSPTNNYGPSG---ASIS 470
           L + K LGVN V L P  +F+E E            Y   +FFSP   Y   G       
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRD 338

Query: 471 AINSMKEMVKELHANGLEVILEVVYTHTSVDG------ALQGIDDSSYYFTNRVANLEEK 530
           AIN  K  V+E H  G+EVI++VV+ HT+         + +GID+S+YY           
Sbjct: 339 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNY 398

Query: 531 S----ALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFLLRG--------------- 590
           S      NCN+P+V++ ++D LRYWVTE HVDGF F  AS + RG               
Sbjct: 399 SGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVE 458

Query: 591 ----HHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKF 650
                 G  L  PP ++ I+ DP+L   KL+A+ WD   L  +  +FPHWK W+E N K+
Sbjct: 459 GDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLY-QVGQFPHWKIWSEWNGKY 518

Query: 651 CSDIRDFFRG-EGLISSLATRLCGSGDVF-SDGRGPAFSFNLIARNVGLPLVDLVSFSNS 710
              +R F +G +G     A  LCGS  ++ + GR P  S N +  + G  L DLV+++  
Sbjct: 519 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 578

Query: 711 NLAS-----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 770
             +S            LSWNCGEEG  + L V   R +Q+RNF   L VS GVP+  MGD
Sbjct: 579 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 638

Query: 771 ECGQSSGG--SVAFNDR--RSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLK 830
           E G + GG  +   +D     F WD  K +  +   +F + ++ FR ++ +     +F  
Sbjct: 639 EYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPT 698

Query: 831 GENIDWFDNNQSPPQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSES 857
            + + W  +    P W + S +F+A             + K +T+ +I++ FNAS     
Sbjct: 699 AQRLHWHGHQPGKPDWSETS-RFVA------------FSTKDETKGEIYVAFNASHLPAV 758

[Close]
BLAST of Chy1G016290 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1726.5 bits (4470), Expect = 0.0e+00
Identity = 850/884 (96.15%), Postives = 867/884 (98.08%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC YNCGVTES KLA SDHLTYGQKTKYQFGKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
           TFTVNIE+ SMQLVSI EALLLSWGVYRSDSALVTPNF+SSPPDET  ATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSS+IRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GS+NFSIFSS+ ESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGYQCKGSKSHENQDGL+VSRIVVDPYAKILAPSIPKSSGQGL LPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANG+EVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           TS +GALQGIDDSSYYFTNRVANLEEKSALNCNYP+VQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NASFLLRGHHGELL RPPFVEAIAFDPLLSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKE+NESITE PKTRS+IFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884

[Close]
BLAST of Chy1G016290 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1679.5 bits (4348), Expect = 0.0e+00
Identity = 829/884 (93.78%), Postives = 847/884 (95.81%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC +NCGVTESLKLAVSDHLTYGQKTKYQ GKMDEARMLAHGESKVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            FTVNIEISSMQLVS  E LLLSW VYRSDSA VT NFQSSPPD T  ATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSS+IRSHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGL+VSRIVVDPYAKILAPSIPKS  QGL  P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANG+EVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           TSVDGALQGIDDSSYYF NRVANLEE+SALNCNYP+VQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NASFLL+GHHGELL RPPFVEAIAFDP+LSKTKLVADFWDPQEL+SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKE+NESITE PKTRSDIFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

[Close]
BLAST of Chy1G016290 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1677.9 bits (4344), Expect = 0.0e+00
Identity = 828/884 (93.67%), Postives = 846/884 (95.70%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC +NCGVTESLKLAVSDHLTYGQKTKYQ GKMDEARMLAHGESKVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            FTVNIEISSMQLVS  E LLLSW VYRSDSA VT NFQSSPPD T  ATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSS+IRSHRKTSFSVPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG S FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGL+VSRIVVDPYAKILAPSIPKS  QGL  P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANG+EVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           TSVDGALQGIDDSSYYF NRVANLEE+SALNCNYP+VQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NASFLL+GHHGELL RPPFVEAIAFDP+LSKTKLVADFWDPQEL+SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKE+NESITE PKTRSDIFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

[Close]
BLAST of Chy1G016290 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 738/884 (83.48%), Postives = 800/884 (90.50%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSL IQPFC YN G TES KLAVSD+ TYGQK  YQ GK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
            KS HRN+ + YA S ISVGKSGQ+ G GGK++E RKVATYLFRTEFGDLV  FVGK+GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            F  +IEISS++L SI E LLLSWGVYRS+SA+VTPNFQS  P+ET  ATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSS+I+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGY+C+GSK HENQD  +VSRI+VDPYAKIL  SIPK+ GQGL LPSKFLGQI  VP F 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+YRLNV+ FT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANG+EVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           T++DGALQGIDDS YYF NRVAN E KSALNCN+P+VQQLLLDSLR+WVTEFH+DGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NAS LL+GHHGELL RPPFVEAIAFDPLLSKTK++ADFWDPQE + KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAF DR+SFNWD LKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           P WED SCKFLAVMLRADKE++ SITE PK +SDIFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           R+VDTAL FPGFFSSDGE VP T  VTY +QAHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 883

[Close]
BLAST of Chy1G016290 vs. ExPASy TrEMBL
Match: A0A6J1GN63 (isoamylase 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1485.7 bits (3845), Expect = 0.0e+00
Identity = 732/884 (82.81%), Postives = 792/884 (89.59%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSL IQPFC YN G TES KLAVSD+ TYGQK  YQ GK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
            KS HRN+ + YA S ISV          GKS+E RKVATYLFRTEFGDLV  FVGK+GS
Sbjct: 61  TKSLHRNVCRAYAPSRISV----------GKSEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            F  +IEISS++L SI E LLLSWGVYRS+SA+VTPNFQS  P+ET  ATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSS+I+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGY+C+GSK HENQD  +VSRI+VDPYAKIL  SIPK+ GQGL LPSKFLGQI  VP F 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+YRLNV+ FT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANG+EVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           T++DGALQGIDDS YYF NRVAN E KSALNCN+P+VQQLLLDSLR+WVTEFH+DGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NAS LL+GHHGELL RPPFVEAIAFDPLLSKTK++ADFWDPQE + KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAF DR+SFNWD LKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           P WED SCKFLAVMLRADKE++ SITE PK +SDIFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           R+VDTAL FPGFFSSDGE VP T  VTY +QAHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 873

[Close]
BLAST of Chy1G016290 vs. NCBI nr
Match: XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])

HSP 1 Score: 1719 bits (4453), Expect = 0.0
Identity = 850/884 (96.15%), Postives = 867/884 (98.08%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC YNCGVTES KLA SDHLTYGQKTKYQFGKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
           TFTVNIE+ SMQLVSI EALLLSWGVYRSDSALVTPNF+SSPPDET  ATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSS+IRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GS+NFSIFSS+ ESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGYQCKGSKSHENQDGL+VSRIVVDPYAKILAPSIPKSSGQGL LPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANG+EVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           TS +GALQGIDDSSYYFTNRVANLEEKSALNCNYP+VQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NASFLLRGHHGELL RPPFVEAIAFDPLLSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKE+NESITE PKTRS+IFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884
           RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884

[Close]
BLAST of Chy1G016290 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1672 bits (4331), Expect = 0.0
Identity = 829/884 (93.78%), Postives = 847/884 (95.81%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC +NCGVTESLKLAVSDHLTYGQKTKYQ GKMDEARMLAHGESKVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            FTVNIEISSMQLVS  E LLLSW VYRSDSA VT NFQSSPPD T  ATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSS+IRSHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGL+VSRIVVDPYAKILAPSIPKS  QGL  P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANG+EVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           TSVDGALQGIDDSSYYF NRVANLEE+SALNCNYP+VQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NASFLL+GHHGELL RPPFVEAIAFDP+LSKTKLVADFWDPQEL+SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKE+NESITE PKTRSDIFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

[Close]
BLAST of Chy1G016290 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1671 bits (4327), Expect = 0.0
Identity = 828/884 (93.67%), Postives = 846/884 (95.70%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC +NCGVTESLKLAVSDHLTYGQKTKYQ GKMDEARMLAHGESKVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            FTVNIEISSMQLVS  E LLLSW VYRSDSA VT NFQSSPPD T  ATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSS+IRSHRKTSFSVPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG S FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGL+VSRIVVDPYAKILAPSIPKS  QGL  P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANG+EVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           TSVDGALQGIDDSSYYF NRVANLEE+SALNCNYP+VQQLLLDSLRYWVTEFHVDGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NASFLL+GHHGELL RPPFVEAIAFDP+LSKTKLVADFWDPQEL+SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKE+NESITE PKTRSDIFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

[Close]
BLAST of Chy1G016290 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1567 bits (4058), Expect = 0.0
Identity = 775/884 (87.67%), Postives = 817/884 (92.42%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFC YN GVTES KLAV DH TYGQK KY  GKMDEARMLAHGES+VG+
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
            KSSHRNLSK YA SGI VGKSGQR G GGKSKE  KVATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            F VN+EISS+QL SI + LLLSWGVYRSDSA+VTPNFQSS PDET  ATETPFVKTS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKHTPFYLSF+LKYP+GVDSGSS+IRSHRKTSF+VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKP+LELDLD YINRSG+IWHASFE AS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGY+CKGSK HENQDG++V+RIVVDPYAKIL  S+ K+ GQGL LP KFLGQI KVP FD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLP-KFLGQICKVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLF YRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIFQFDEKEG YFPFHFFSP NNYGPSG SISAINSMK MVK+LHANG+EVILEVV+TH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           T++DGALQGIDDSSYYF NRVANLE KSALNCNYP+VQQLLLDSLRYWVTEFH+DGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NAS LLRGHHGE L R PFVEAIAFDPLLSKTKLVADFWDPQEL+S+ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKFC+D+RDFFRGEGLISSLATRLCGSGDVFS+GRGPAFSFN I RNVGLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NS LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFV+FVSLGVPVLNMGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWED SCKFLAVMLRADKE+N SITE  K RSDIFM FNASDQSE+VALPEP EGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884
           R+VDTAL FPGFFSSDGE VP TGSVTYE+QAHSCALFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFN 883

[Close]
BLAST of Chy1G016290 vs. NCBI nr
Match: KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1503 bits (3891), Expect = 0.0
Identity = 736/884 (83.26%), Postives = 803/884 (90.84%), Query Frame = 0 

Query: 1   MATFLSSLVIQPFCAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGA 60
           MATFLSSL IQPFC YN G TES KLAV+D+ TYGQK  YQ GK+DEARM AHGESKVG 
Sbjct: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKKGS 120
            KS HRN+ K YA SGISVGKSGQ+ G GGK++E RKVATYLFRTEFGDLV  FVGK+GS
Sbjct: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143

Query: 121 TFTVNIEISSMQLVSIGEALLLSWGVYRSDSALVTPNFQSSPPDETAEATETPFVKTSEG 180
            F V+IEISS++L SI E LLLSWGVYRS+SA+VTPNFQS  P+ET  ATETPFV+TSEG
Sbjct: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSS+I+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263

Query: 241 SGGGSINFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SG GSINFSIFSST ES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWH SFEGAS FVS
Sbjct: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323

Query: 301 YGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFD 360
           YGY+C+GSK HENQD  +VSRI+VDPYAKIL  S PK+ GQG+ LPSKFLGQI  VP F 
Sbjct: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+YRLNV+ FT+DKSSQLP DIAGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANG+EVILEVV+TH
Sbjct: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503

Query: 481 TSVDGALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFI 540
           T++DGALQGIDDS YYF NRVAN E KSALNCN+P+VQQLLLDSLRYWVTEFH+DGFCFI
Sbjct: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563

Query: 541 NASFLLRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEV 600
           NAS LL+GHHGELL RPPFVEAIAFDPLLSKTK++A+FWDPQEL+ KETRFPHWKRWAE+
Sbjct: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFS 660
           NSKF +D+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFN IARNVGLPLVDLVSFS
Sbjct: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAF DR+SFNWD LKTDFGTQTT+FI F SSFRSRRFDLFQSR+FLKGENIDWFDNNQSP
Sbjct: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803

Query: 781 PQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWF 840
           P+WED SCKFLAVMLRADKE++ SITE PK +SDIFM FNASDQS SVALPEP EGT+WF
Sbjct: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884
           R+VDTAL FPGFFSSDGE VP T  VTYE+QAHSC LFEAKS N
Sbjct: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFN 906

[Close]
BLAST of Chy1G016290 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 929.5 bits (2401), Expect = 2.0e-270
Identity = 471/882 (53.40%), Postives = 625/882 (70.86%), Query Frame = 0 

Query: 14  CAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGAVKSSHRNLSKTYA 73
           C  N G+T + K   S  L  G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKFP-SARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRKVATYLFRTEFGDLVNVFVGKKGSTFTVNIEI 133
            S I   K+   L     S+     + +KV +Y FRT+ G LV V V KK   +++ + +
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 SSMQLVSIGEA-LLLSWGVYRSDSALVTP-NFQSSPPDETAEATETPFVKTSEGKFSVEL 193
           SS++L    ++ L++ WGVYRSDS+   P +F++S  D     TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSIS-GGGS 253
           EFD K +PFYLSF LK   G D    ++ +HR T F +PVGF  G+P PLGLS      S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 INFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS ++ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFDWDGEV 373
           K +   +    ++   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 RPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTHTSVDG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+  G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCFINAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFC 613
           LRG HGE L RPP VEAIAFDPLL++TKL+AD WDP E++ KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 RRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPPQWED 793
           R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEDNESIT----EKPKTRSDIFMVFNASDQSESVALPEPLEGTSWFR 853
            + KFLA+ ++++ E+ E+ +     +PK+ +D+F+ FNASD  ESV LP   +G+ W R
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKS-NDLFIGFNASDHPESVVLPSLPDGSKWRR 852

Query: 854 VVDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           +VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 LVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

[Close]
BLAST of Chy1G016290 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 929.5 bits (2401), Expect = 2.0e-270
Identity = 471/882 (53.40%), Postives = 625/882 (70.86%), Query Frame = 0 

Query: 14  CAYNCGVTESLKLAVSDHLTYGQKTKYQFGKMDEARMLAHGESKVGAVKSSHRNLSKTYA 73
           C  N G+T + K   S  L  G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKFP-SARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRKVATYLFRTEFGDLVNVFVGKKGSTFTVNIEI 133
            S I   K+   L     S+     + +KV +Y FRT+ G LV V V KK   +++ + +
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 SSMQLVSIGEA-LLLSWGVYRSDSALVTP-NFQSSPPDETAEATETPFVKTSEGKFSVEL 193
           SS++L    ++ L++ WGVYRSDS+   P +F++S  D     TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSKIRSHRKTSFSVPVGFGRGYPSPLGLSIS-GGGS 253
           EFD K +PFYLSF LK   G D    ++ +HR T F +PVGF  G+P PLGLS      S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 INFSIFSSTTESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS ++ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSKFLGQISKVPTFDWDGEV 373
           K +   +    ++   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 RPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGLEVILEVVYTHTSVDG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+  G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCFINAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLFRPPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFC 613
           LRG HGE L RPP VEAIAFDPLL++TKL+AD WDP E++ KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 RRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPPQWED 793
           R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEDNESIT----EKPKTRSDIFMVFNASDQSESVALPEPLEGTSWFR 853
            + KFLA+ ++++ E+ E+ +     +PK+ +D+F+ FNASD  ESV LP   +G+ W R
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKS-NDLFIGFNASDHPESVVLPSLPDGSKWRR 852

Query: 854 VVDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           +VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 LVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

[Close]
BLAST of Chy1G016290 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 367.9 bits (943), Expect = 2.4e-101
Identity = 244/701 (34.81%), Postives = 357/701 (50.93%), Query Frame = 0 

Query: 231 GYPSPLGLSISGGGSINFSIFSSTTESLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S    G INF++FS    S+ LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVSQVDKG-INFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLR 350
           + WH   E      V YGY+  G    +       S +++DPYAK++      S G   +
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 LPSKFLGQIS-KVPTFDWDGEVR-PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
             ++F G    +   FDW  + + PN+P + L +Y +NV  FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
             +K+ H ++LG+NAV L P+F+FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 SGASISAINSMKEMVKELHANGLEVILEVVYTHTS-------VDGALQGIDDSSYYF--- 530
            G  I A    KEMVK LH+ G+EVIL+VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 TNRVANLEE-KSALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFLLRGHHGELLFR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R   G  L  
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNLIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    N +  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRR-FDLFQSRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + +  NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFNASDQSESVALPEPLEGTSWFR 882
            W+++  KFLA  L      ++ I  +     DI++ FNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

[Close]
BLAST of Chy1G016290 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 334.7 bits (857), Expect = 2.2e-91
Identity = 230/690 (33.33%), Postives = 334/690 (48.41%), Query Frame = 0 

Query: 231 GYPSPLGLSISGGGSINFSIFSSTTESLVLCL--YNDSTSEKPLLELDLDPYINRSGNIW 290
           G PSP G ++   G +NFS++S+ + S  +CL   +D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLKVSRIVVDPYAKILAPSIPKSSGQGLRLPSK 350
           H    G  K + YGY+  G  S E       S I++DPYAK +   I +     L     
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAI---ISRDEFGVLGPDDN 191

Query: 351 FLGQIS-KVPT----FDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGL 410
              Q++  VPT    FDW+G++   LP + L +Y ++V  FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 411 TKKLLHFKNLGVNAVLLEPIFQFDEKE---------------GPYFPFHFFSPTNNYGPS 470
            +KL H K LG+N + L P  +F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 471 GASI---SAINSMKEMVKELHANGLEVILEVVYTHTSVDG------ALQGIDDSSYYFTN 530
            ++     AIN  K +VKE H  G+EVI++VV  HT+         + +G+D+S YY   
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 531 RVANLEEKS----ALNCNYPMVQQLLLDSLRYWVTEFHVDGFCFINASFLLRGH------ 590
                   S      NCN+P+V+Q +LD LRYWVTE HVDGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 591 -------HGELLFR------PPFVEAIAFDPLLSKTKLVADFWDPQELISKETRFPHWKR 650
                   G+LL        PP ++ I+ DP+L   KL+A+ WD   L  +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHWGI 491

Query: 651 WAEVNSKFCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNLIARNVGLPLVD 710
           W+E N KF   +R F +G +G   + A  LCGS +++  GR P  S N I  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 711 LVSFSN-SNLAS----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGV 770
           LV+++N +NLA+            SWNCGEEG  +++ V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 771 PVLNMGDECGQSSGGS----VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLF 830
           P++ MGDE G + GG+       N    F WD  K +  +   +F   L  FR    +  
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRD-ECESL 671

Query: 831 QSRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEDNESITEKPKTRSDIFMVFN 851
              +F   + + W       P W + S +F+A  L                + +I++ FN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL------------VDSVKKEIYVAFN 731

[Close]
BLAST of Chy1G016290 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 57.0 bits (136), Expect = 8.9e-08
Identity = 43/161 (26.71%), Postives = 78/161 (48.45%), Query Frame = 0 

Query: 399 TFSGLTKKLL-HFKNLGVNAVLLEPIFQF-DEKEGPYFPFHFFSPTNNYGPSGASISAIN 458
           TF   TKK+L H K  G NA+ L  + +  D     Y   +FF+ ++ YG         +
Sbjct: 416 TFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------D 475

Query: 459 SMKEMVKELHANGLEVILEVVYTHTSVD-----GALQGIDDSSYYFTNRVANLEEKSALN 518
             K +V E H  GL V L++V+++ + D         G +D  +++  R  +    + + 
Sbjct: 476 DFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMF 535

Query: 519 CNYPM-VQQLLLDSLRYWVTEFHVDGFCFINASFLLRGHHG 552
               + V   L+ +L +W+TE+ VDG+ F + + ++  H+G
Sbjct: 536 KYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNG 569

The following BLAST results are available for this feature:
BLAST of Chy1G016290 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L7352.9e-26953.40Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2[more]
Q6AU804.8e-21651.54Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=... [more]
Q9M0S53.3e-10034.81Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2[more]
B9G4342.8e-9935.29Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... [more]
D0TZF01.0e-9333.38Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... [more]
BLAST of Chy1G016290 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K5510.0e+0096.15Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 S... [more]
A0A1S3BRF80.0e+0093.78isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1[more]
A0A5A7UR470.0e+0093.67Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 P... [more]
A0A6J1GPU30.0e+0083.48isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1GN630.0e+0082.81isoamylase 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
BLAST of Chy1G016290 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004136188.10.096.15isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein C... [more]
XP_008451396.10.093.78PREDICTED: isoamylase 2, chloroplastic [Cucumis melo][more]
KAA0057598.10.093.67isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo v... [more]
XP_038898155.10.087.67isoamylase 2, chloroplastic [Benincasa hispida][more]
KAG7014422.10.083.26Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Chy1G016290 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G03310.22.0e-27053.40debranching enzyme 1 [more]
AT1G03310.12.0e-27053.40debranching enzyme 1 [more]
AT4G09020.12.4e-10134.81isoamylase 3 [more]
AT2G39930.12.2e-9133.33isoamylase 1 [more]
AT3G20440.28.9e-0826.71Alpha amylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 375..753
e-value: 0.001
score: -73.8
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 398..486
e-value: 3.5E-9
score: 36.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 355..768
e-value: 2.4E-99
score: 335.3
NoneNo IPR availablePANTHERPTHR43002:SF6ISOAMYLASE 2, CHLOROPLASTICcoord: 72..883
NoneNo IPR availablePANTHERPTHR43002GLYCOGEN DEBRANCHING ENZYMEcoord: 72..883
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 769..881
IPR004193Glycoside hydrolase, family 13, N-terminalPFAMPF02922CBM_48coord: 235..328
e-value: 1.4E-10
score: 41.4
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 769..885
e-value: 4.5E-26
score: 93.2
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 221..341
e-value: 1.1E-24
score: 88.5
IPR044096Isoamylase 2, catalytic domainCDDcd11346AmyAc_plant_IsoAcoord: 370..761
e-value: 6.8979E-148
score: 438.444
IPR044505Glycogen debranching enzyme GlgX/isoamylase, N-terminal Early set domainCDDcd02856E_set_GDE_Isoamylase_Ncoord: 234..378
e-value: 9.22833E-23
score: 92.7096
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 356..755
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 231..364

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G016290.1Chy1G016290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005977 glycogen metabolic process
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0019156 isoamylase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds