Chy12G212540 (gene) Cucumber (hystrix) v1

BLAST of Chy12G212540 vs. ExPASy Swiss-Prot
Match: B4F6I3 (Ferroptosis suppressor protein 1 OS=Xenopus tropicalis OX=8364 GN=aifm2 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.2e-37
Identity = 103/365 (28.22%), Postives = 184/365 (50.41%), Query Frame = 0

Query: 11  RVAVVGGGIAGSLVAKSLQFFA-HITLIDPKEYLEIPYGSLRSMVEPCFAERMLINHSHY 70
           RV +VGGG AG   A  L+ F     L+D K+       +LR+ VE  FA +  I++   
Sbjct: 12  RVVIVGGGFAGIAAATQLKSFGIPFVLVDLKDAFHHNVAALRASVESGFARKTFISYKDT 71

Query: 71  FSN----GRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDP-----SLPITRTHRL 130
           F +    G+++    +++   +V++     + F  +++ATG + P     +  I++   +
Sbjct: 72  FQDNFIQGKVV---GINLQTQRVILESNEELQFSHLIIATGSNGPFPGKINNVISKDQAI 131

Query: 131 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEF-MGPKAS 190
             Y    + I++A+ ++++GGG  G+E+A E+ T++P+K +TLVH    + +  + PK  
Sbjct: 132 QVYEDLVKEIQKAKHVVVVGGGSAGVEMAAEVKTDYPEKEVTLVHSKVALADVQLQPKVR 191

Query: 191 EKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSS------KGEIIRADCHFVCTGKAVA 250
                 L+SK V + L Q +   D    N +  ++        E++  D    CTG  ++
Sbjct: 192 RTVKEILLSKGVRLILAQKVTNLDQVTSNVAQENTVLQLDKNSEVVTCDLVLCCTGYKIS 251

Query: 251 SAWLRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAK 310
           S+    S     L  +G L+V+ YL+V+G  N++A+GD   I E K    A   A+VAA 
Sbjct: 252 SS-SYSSAFGDKLAEDGALIVNDYLQVQGHANVYAVGDCAYINEPKMAYYAGIHARVAAT 311

Query: 311 NLKMLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNT 359
           N++  ++GK  K  +  A    + ++S+GR   V QF+   +        KSRD+FV  +
Sbjct: 312 NVRNSLIGKSLKTYKPGA---LSMLLSMGRNDGVGQFNGCYLGRFFVTMAKSRDIFVSKS 369

BLAST of Chy12G212540 vs. ExPASy Swiss-Prot
Match: Q6GLW8 (Ferroptosis suppressor protein 1 OS=Xenopus laevis OX=8355 GN=aifm2 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.1e-36
Identity = 102/365 (27.95%), Postives = 182/365 (49.86%), Query Frame = 0

Query: 11  RVAVVGGGIAGSLVAKSLQFFA-HITLIDPKEYLEIPYGSLRSMVEPCFAERMLINHS-- 70
           RV +VGGG AG   A  L+ F     L+D K+       +LR+ VE  FA +  I++   
Sbjct: 12  RVVIVGGGFAGIAAASQLKSFGIPFLLVDMKDAFHHNVAALRACVESGFARKTFISYKDS 71

Query: 71  --HYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDP-----SLPITRTHRL 130
               F  G+++    +++    V++     + F  +++ATG + P     +  I++   +
Sbjct: 72  FHDSFKQGKVV---GINLQTQLVILESNEELSFSHLIIATGSNGPFPGKFNEVISKDQAI 131

Query: 131 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEF-MGPKAS 190
             Y    E I++A+ ++++GGG  G+E+A E+ T++P+K +TL+H    + +  + P   
Sbjct: 132 QIYENLVEEIQKAKHVVVVGGGSAGVEMAAEVKTDYPEKEVTLIHSKIALADVQLQPSVR 191

Query: 191 EKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSK------GEIIRADCHFVCTGKAVA 250
                 L+ K V + L Q +   D    N +  + +       E++  D    C G  V+
Sbjct: 192 RTVKEILLRKGVRLILAQKVTNLDQVTPNVAQENMELQLDKDSEVVNCDLVLCCIGLKVS 251

Query: 251 SAWLRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAK 310
           S+  R S L   +  +G L+V+ YL+V+G  N++A+GD   I E K    A   A+VAA 
Sbjct: 252 SSSYR-SALGDKMAEDGSLIVNDYLQVQGHENVYAVGDCAYINEPKMAYYAGIHARVAAT 311

Query: 311 NLKMLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNT 359
           N++  ++GK   +K Y+     + ++S+GR   V QF+   +        KSRD+FV  +
Sbjct: 312 NVRNSLIGK--SLKSYIPGA-LSMLLSMGRNDGVGQFNGYYLGRCFVTMAKSRDIFVSKS 369

BLAST of Chy12G212540 vs. ExPASy Swiss-Prot
Match: A5PJM4 (Ferroptosis suppressor protein 1 OS=Bos taurus OX=9913 GN=AIFM2 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-34
Identity = 114/366 (31.15%), Postives = 183/366 (50.00%), Query Frame = 0

Query: 12  VAVVGGGIAGSLVAKSLQFF-AHITLIDPKEYLEIPYGSLRSMVEPCFAERMLINHSHYF 71
           V +VGGG  G   A  LQ       L+D K+       +LR+ VE  FA++  I++S  F
Sbjct: 14  VVIVGGGFGGIAAASQLQALNIPFVLVDMKDSFHHNVAALRASVESGFAKKTFISYSVTF 73

Query: 72  -SNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDP-----SLPITRTHRLHQYT 131
             N R      +D+    VL+ DG+ + F  +++ATG         +   ++   +  Y 
Sbjct: 74  KENFRQGLVVEIDLKNQTVLLEDGQALPFSHLILATGSTGLFPGKFNQVSSQQMAIQAYE 133

Query: 132 AENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDG---------PRVLEFMG 191
               +++R++SI+++GGG  G+E+A EI TE+P+K +TL+H           P V + + 
Sbjct: 134 DMVTQVQRSQSIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSKMALADTELLPCVRQEVK 193

Query: 192 PKASEKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASA 251
                K ++ L+S+RV+  LE  + VN+  +  K  ++ KG  + A+   VC G  + SA
Sbjct: 194 EILLRKGVQLLLSERVS-NLE-ALPVNERRECIK-VQTDKGTEVDANLVIVCNGIKINSA 253

Query: 252 WLRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNL 311
             R S     L +NG L V+ YL+V+G  +I+AIGD  D+RE K    A   A VA  N+
Sbjct: 254 AYR-SAFGDRLASNGALRVNEYLQVEGYSHIYAIGDCADVREPKMAYHASLHANVAVANI 313

Query: 312 KMLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRK 362
              M  K   +K Y   +  T ++++GR   V Q S   +  ++    KSRDL V  + K
Sbjct: 314 VNSM--KQRPLKTYKPGS-LTFLLAMGRNDGVGQISGFYVGRLMVRLAKSRDLLVSTSWK 372

BLAST of Chy12G212540 vs. ExPASy Swiss-Prot
Match: Q54NS8 (Apoptosis-inducing factor homolog B OS=Dictyostelium discoideum OX=44689 GN=aifB PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.9e-34
Identity = 106/376 (28.19%), Postives = 188/376 (50.00%), Query Frame = 0

Query: 10  RRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAERMLINHSHY 69
           +RV ++GGG  G  VAK L    ++T+++ K+      GS+R++VEP   +++ I +   
Sbjct: 6   KRVLIIGGGYGGCEVAKQLDSKFNVTVVERKQTFFHSVGSVRAVVEPELVKKIYIPYDKL 65

Query: 70  FSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHD------PSLPITRTHRLHQY 129
             NG+ I    ++++ +   + DG+ + FD++V+ATG +       P    + +  L+ +
Sbjct: 66  LKNGKFIFGTVIEISPTLAKLEDGQELTFDYLVIATGSNSLAPFKAPLEKKSSSEILNYF 125

Query: 130 TAENERIRRAESILIIGGGPTGIELAGEITTEFP------DKAITLVHDGPRVLE-FMGP 189
              +++I++A+SILI+GGG    EL  EI  ++P       K IT+VH G +++   M  
Sbjct: 126 QNFSQQIKQAKSILIVGGGAVACELVSEIVEKYPVKDSELVKKITIVHSGSKLVNPKMND 185

Query: 190 KASEKALRWLISKRVNVKLEQTIDVNDMWDGN-------------KSFRSSKGEIIRADC 249
           K +    + +  + V V L   I + D    N             +++ + KG  I+AD 
Sbjct: 186 KFTNVVSKAMKKRNVEVILNDRITMPDEIKANLLNQTSPNIQISSQNYTTEKGVPIQADL 245

Query: 250 HFVCTGKAVASAWLRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGES 309
             + T     ++   +S     ++ +G+L V+   +V+G +N+FAIGD TD  E K   +
Sbjct: 246 -IIWTVGIKTNSESYQSHFSNVINESGQLKVNLSCQVQGYNNVFAIGDCTDFDEFKTAYN 305

Query: 310 AKRQAKVAAKNLKMLMVGK-DSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISG-ILPA 358
           A   A +AAK +  L  GK + K+ ++    P  ++ SLG +  + Q S T   G     
Sbjct: 306 AGYHAAIAAKAIDALSKGKSNDKLAKHKVSGPILSL-SLGPQDGITQISPTMCLGSFATK 365

BLAST of Chy12G212540 vs. ExPASy Swiss-Prot
Match: B5FXE5 (Ferroptosis suppressor protein 1 OS=Taeniopygia guttata OX=59729 GN=AIFM2 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-33
Identity = 105/364 (28.85%), Postives = 183/364 (50.27%), Query Frame = 0

Query: 11  RVAVVGGGIAGSLVAKSLQFFA-HITLIDPKEYLEIPYGSLRSMVEPCFAERMLINHS-- 70
           RV VVGGG  G+  A  L+ +A    L+D ++       +LR+ VE  FA++  I++S  
Sbjct: 12  RVVVVGGGFGGTAAASLLKSWAVPFVLVDVRDAFHHNVAALRAAVESGFAKKTFISYSVT 71

Query: 71  --HYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDP-----SLPITRTHRL 130
               F  G+++   A+D    QV+++DG  + +  +++ATG   P     +  I     +
Sbjct: 72  FGDSFRQGKVV---AIDPGRQQVVLSDGEELHYSHLILATGSDGPFPGKFNQVIDMESAI 131

Query: 131 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEF-MGPKAS 190
             Y    + I +++ IL++GGG  G+E+A EI TE+P K I L+H    + +  + P   
Sbjct: 132 QTYEDMVKEIEKSQRILVVGGGAAGVEMAAEIKTEYPGKEIILIHSKTALADVELLPSVR 191

Query: 191 EKALRWLISKRVNVKL-EQTIDVNDM----WDGNKSFRSSKGEIIRADCHFVCTGKAVAS 250
           +     L+ K V + L E+  D+ ++    +  +   R+ KG  +  D   +CTG  + S
Sbjct: 192 QVVKEILLRKGVRLLLSEKVSDIENLRPNQFQKDMVVRTEKGTEVVVDMVVLCTGIKINS 251

Query: 251 AWLRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKN 310
           +    +   K + ++G L V+ +L+++G  NI+AIGD  D++E K    A   A V   N
Sbjct: 252 SAYAAAFGDK-MASDGALKVNKHLQLEGYENIYAIGDCADLKEPKMAYHAGLHANVVVTN 311

Query: 311 LKMLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTR 359
           +   +  K   +K Y   +  T ++S+GR   V Q +   +  +L    KSRDLFV  + 
Sbjct: 312 IINSLTQK--PLKTYEPGS-LTFLLSMGRNDGVGQVNGYYVGRLLVTIAKSRDLFVSKSW 368

BLAST of Chy12G212540 vs. ExPASy TrEMBL
Match: A0A0A0LSU3 (Pyr_redox_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G169990 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 7.1e-201
Identity = 356/365 (97.53%), Postives = 362/365 (99.18%), Query Frame = 0

Query: 1   MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE 60
           MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE
Sbjct: 1   MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE 60

Query: 61  RMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRL 120
           RMLINHSHYFSNGRLITSPAV VTESQVLVADGR+VDFDFVVVATGHHDPSLPITRTHRL
Sbjct: 61  RMLINHSHYFSNGRLITSPAVGVTESQVLVADGRNVDFDFVVVATGHHDPSLPITRTHRL 120

Query: 121 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE 180
           HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE
Sbjct: 121 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE 180

Query: 181 KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKS 240
           KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEII ADCHFVCTGKAVAS+WL+KS
Sbjct: 181 KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIIADCHFVCTGKAVASSWLQKS 240

Query: 241 ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV 300
           ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV
Sbjct: 241 ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV 300

Query: 301 GKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLH 360
           GK+ KM+RYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRK+LGLH
Sbjct: 301 GKEGKMERYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKKLGLH 360

Query: 361 PTLLD 366
           PTLLD
Sbjct: 361 PTLLD 365

BLAST of Chy12G212540 vs. ExPASy TrEMBL
Match: A0A6J1HVR8 (apoptosis-inducing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466595 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 8.2e-157
Identity = 283/369 (76.69%), Postives = 320/369 (86.72%), Query Frame = 0

Query: 1   MDVHG----GGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEP 60
           MD HG    GG LRRV VVGGGIAGSL+AKSLQFFA++TLIDPKEYLEIPYGSLRS VEP
Sbjct: 1   MDEHGGGGCGGGLRRVVVVGGGIAGSLLAKSLQFFANVTLIDPKEYLEIPYGSLRSTVEP 60

Query: 61  CFAERMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITR 120
            FAER LI HS YFSNGRLITSPAVD+TESQVLVADGRHVDFDF+VVATGH  P LP TR
Sbjct: 61  SFAERSLIKHSEYFSNGRLITSPAVDITESQVLVADGRHVDFDFLVVATGHDHP-LPTTR 120

Query: 121 THRLHQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGP 180
            HRL  Y  ENERIR A SILI+GGGP+G+ELAGEI T+F DKAITLVHDGPR+LEFMGP
Sbjct: 121 DHRLQHYREENERIREANSILIVGGGPSGVELAGEIVTDFRDKAITLVHDGPRLLEFMGP 180

Query: 181 KASEKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAW 240
           KAS+KAL+WL+S+RV VKL+Q ID+N++ +G K+F SSKGE IRADCHFVCTG+ V SAW
Sbjct: 181 KASDKALKWLVSRRVAVKLDQKIDLNNVSEGTKTFGSSKGETIRADCHFVCTGQPVGSAW 240

Query: 241 LRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLK 300
           L K++LK +LD +GRLMVD  LRVKG++N+FAIGDITDIRESKQGESAKRQAKVAAKN+K
Sbjct: 241 LHKTMLKINLDRHGRLMVDGTLRVKGRNNVFAIGDITDIRESKQGESAKRQAKVAAKNMK 300

Query: 301 MLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQ 360
           M+MVGK+SKM+ Y   + TTA+VSLGRKQAVAQFS+TTI+G LP FIKSRDLFVG  RK 
Sbjct: 301 MIMVGKESKMESYRPPSSTTAIVSLGRKQAVAQFSLTTIAGTLPGFIKSRDLFVGKARKH 360

Query: 361 LGLHPTLLD 366
           LGLHPT +D
Sbjct: 361 LGLHPTFVD 368

BLAST of Chy12G212540 vs. ExPASy TrEMBL
Match: A0A6J1G1H9 (apoptosis-inducing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449831 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 5.3e-156
Identity = 282/366 (77.05%), Postives = 318/366 (86.89%), Query Frame = 0

Query: 1   MDVH-GGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFA 60
           MD H GGG LRRV VVGGGIAGSL+AKSLQFFA++TLIDPKEYLEIPYGSLRS VEP FA
Sbjct: 1   MDEHGGGGGLRRVVVVGGGIAGSLLAKSLQFFANVTLIDPKEYLEIPYGSLRSTVEPSFA 60

Query: 61  ERMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHR 120
           ER LI HS YFSNGRLITSPAVD+TESQVLVADGRHVDFDF+VVATGH  P LP TR  R
Sbjct: 61  ERSLIKHSEYFSNGRLITSPAVDITESQVLVADGRHVDFDFLVVATGHDHP-LPTTRALR 120

Query: 121 LHQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKAS 180
           L  Y  ENERIR A SIL+IGGGP+G+ELAGEI T+F DKAITL+HDGPR+LEFMGPKAS
Sbjct: 121 LQHYREENERIREANSILVIGGGPSGVELAGEIATDFRDKAITLIHDGPRLLEFMGPKAS 180

Query: 181 EKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRK 240
           +KAL+WLIS+RV VKL+Q ID+N++ +G K+FRSSKGE IRADCHFVCTG+ V SAWL K
Sbjct: 181 DKALKWLISRRVAVKLDQKIDLNNISEGTKTFRSSKGETIRADCHFVCTGQPVGSAWLHK 240

Query: 241 SILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLM 300
           + LK +LD +GRLMVD  LRVKG++N+FAIGDITDIRESKQGESAKRQA+VAAKN+KM+M
Sbjct: 241 TTLKTNLDRHGRLMVDGNLRVKGRNNVFAIGDITDIRESKQGESAKRQARVAAKNMKMIM 300

Query: 301 VGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGL 360
           VGK+SKM+ Y   + TTA+VSLGRKQAVAQFS TTI+G LP FIKSRDLFVG  RK LGL
Sbjct: 301 VGKESKMEIYRPPSSTTAIVSLGRKQAVAQFSFTTIAGTLPGFIKSRDLFVGKARKHLGL 360

Query: 361 HPTLLD 366
           HPT +D
Sbjct: 361 HPTFVD 365

BLAST of Chy12G212540 vs. ExPASy TrEMBL
Match: A0A6J1CNB9 (apoptosis-inducing factor homolog B-like OS=Momordica charantia OX=3673 GN=LOC111012591 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.1e-145
Identity = 272/362 (75.14%), Postives = 307/362 (84.81%), Query Frame = 0

Query: 6   GGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAERMLIN 65
           GG+LRRV V+GGGIAGSLVAKSLQFFA++TLIDPKEYLEIPYG LRSMVEP FAER +IN
Sbjct: 5   GGDLRRVVVIGGGIAGSLVAKSLQFFANVTLIDPKEYLEIPYGRLRSMVEPSFAERSVIN 64

Query: 66  HSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRLHQYTA 125
           HS YF+NGRLITSPAVD+ ES+VLVADGRHV FD++VVATG   P LP TR HRL QY A
Sbjct: 65  HSEYFTNGRLITSPAVDINESEVLVADGRHVVFDYLVVATGRDFP-LPKTRAHRLDQYRA 124

Query: 126 ENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASEKALRW 185
           ENE+IR A SILIIGGGP G+ELAGEI T+F DK IT+VH G R++EFMGPKAS+KAL+W
Sbjct: 125 ENEKIRGANSILIIGGGPVGVELAGEIATDFSDKRITVVHGGERLVEFMGPKASDKALKW 184

Query: 186 LISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKSILKKS 245
           L SK+V+VKL++T+ V+DM D  KSFR+S GEII AD HFVCTG  +AS+WLRK+IL  +
Sbjct: 185 LTSKKVDVKLDRTVVVDDMSDETKSFRTSTGEIISADAHFVCTGIPLASSWLRKTILDSN 244

Query: 246 LDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMVGKD-S 305
           LD++GRLMVD YLRVKG+  IFAIGDITDIRE KQGESAKRQAKVA KN+KMLMVGKD S
Sbjct: 245 LDSHGRLMVDGYLRVKGRKKIFAIGDITDIREMKQGESAKRQAKVATKNMKMLMVGKDES 304

Query: 306 KMKRY--VARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLHPT 365
           KM+ Y   A    TA VSLGRKQAVAQ   TTI GILPAF+KSRDLFVGNTRK LGLHPT
Sbjct: 305 KMESYRSGAGAGATASVSLGRKQAVAQLPFTTIKGILPAFLKSRDLFVGNTRKHLGLHPT 364

BLAST of Chy12G212540 vs. ExPASy TrEMBL
Match: A0A6P5XNV2 (apoptosis-inducing factor 2-like OS=Durio zibethinus OX=66656 GN=LOC111284957 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 7.0e-124
Identity = 220/360 (61.11%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 3   VHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAERM 62
           +  GG  +R+ VVGGGIAGSL+AKS+QF A +TLIDPK+YLEI + SLR+MVEP FAER 
Sbjct: 1   MESGGEKKRMVVVGGGIAGSLLAKSIQFNADVTLIDPKDYLEITWASLRNMVEPSFAERS 60

Query: 63  LINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRLHQ 122
           +INH  Y +NGR++TS  +D+T+++VL ++GR + +D++V+ATGH DP +P T+T RL+Q
Sbjct: 61  VINHRDYLTNGRIVTSAVIDITDTEVLTSEGRRIAYDYLVIATGHRDP-VPKTKTERLNQ 120

Query: 123 YTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASEKA 182
           Y AEN++I+ A SI+I+GGGPTG+ELAGEI T+FP+K +TLVH GPR+LEF+G KA++KA
Sbjct: 121 YQAENQKIQSAYSIVIVGGGPTGVELAGEIATDFPEKKVTLVHKGPRLLEFIGAKAADKA 180

Query: 183 LRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKSIL 242
           LRWL S++V VKLEQ +D+N   DG++ +++S GE I+ADCHF+C GK +ASAWL ++IL
Sbjct: 181 LRWLRSRKVEVKLEQAVDLNSASDGSQVYKTSTGESIKADCHFLCAGKPLASAWLNETIL 240

Query: 243 KKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV-G 302
           K +LD +GRLMVD YLRVKG+ NIFAIGDITDI E KQG  A + A V A+NLK+L+  G
Sbjct: 241 KTNLDKSGRLMVDEYLRVKGRSNIFAIGDITDIPELKQGYIAHKHAMVVARNLKLLLAGG 300

Query: 303 KDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLHP 362
           K+SKM +Y   +   A+VSLGRK AVAQF  TTISG +P  +KSRDLFVG TRKQ+GL P
Sbjct: 301 KESKMSKYEPGS-AIALVSLGRKDAVAQFPFTTISGCVPGLVKSRDLFVGRTRKQMGLQP 358

BLAST of Chy12G212540 vs. NCBI nr
Match: XP_004139594.1 (apoptosis-inducing factor 2 [Cucumis sativus] >KGN64975.1 hypothetical protein Csa_022801 [Cucumis sativus])

HSP 1 Score: 706 bits (1821), Expect = 6.07e-256
Identity = 356/365 (97.53%), Postives = 362/365 (99.18%), Query Frame = 0

Query: 1   MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE 60
           MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE
Sbjct: 1   MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE 60

Query: 61  RMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRL 120
           RMLINHSHYFSNGRLITSPAV VTESQVLVADGR+VDFDFVVVATGHHDPSLPITRTHRL
Sbjct: 61  RMLINHSHYFSNGRLITSPAVGVTESQVLVADGRNVDFDFVVVATGHHDPSLPITRTHRL 120

Query: 121 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE 180
           HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE
Sbjct: 121 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE 180

Query: 181 KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKS 240
           KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEII ADCHFVCTGKAVAS+WL+KS
Sbjct: 181 KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIIADCHFVCTGKAVASSWLQKS 240

Query: 241 ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV 300
           ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV
Sbjct: 241 ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV 300

Query: 301 GKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLH 360
           GK+ KM+RYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRK+LGLH
Sbjct: 301 GKEGKMERYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKKLGLH 360

Query: 361 PTLLD 365
           PTLLD
Sbjct: 361 PTLLD 365

BLAST of Chy12G212540 vs. NCBI nr
Match: XP_038895607.1 (ferroptosis suppressor protein 1-like [Benincasa hispida])

HSP 1 Score: 612 bits (1579), Expect = 4.88e-219
Identity = 309/367 (84.20%), Postives = 336/367 (91.55%), Query Frame = 0

Query: 1   MDVHGG--GNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCF 60
           MD   G  G LRRVAVVGGGIAGSL+AKSLQFFAH+TLIDPKEYLEIPYGSLRS VEPCF
Sbjct: 1   MDAPRGSDGGLRRVAVVGGGIAGSLIAKSLQFFAHVTLIDPKEYLEIPYGSLRSTVEPCF 60

Query: 61  AERMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTH 120
           AERMLI+HSHYF+NGRLITSPAVDVTESQVLVADGRHV FDF+VVATGHHD +LP TRT 
Sbjct: 61  AERMLIDHSHYFTNGRLITSPAVDVTESQVLVADGRHVVFDFLVVATGHHDFTLPTTRTQ 120

Query: 121 RLHQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKA 180
           RLHQYT ENE+IRRA+SILIIGGGPTG+ELAGEI TEFPDKAITLVHDGPR+LEF+GPKA
Sbjct: 121 RLHQYTEENEKIRRAKSILIIGGGPTGVELAGEIATEFPDKAITLVHDGPRLLEFIGPKA 180

Query: 181 SEKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLR 240
           S KA RW+ISKRV+VKLEQTID+N+M D   SFRSSKGEIIRADC+FVCTGK VASAWL 
Sbjct: 181 SNKAFRWMISKRVDVKLEQTIDLNNMSDEITSFRSSKGEIIRADCYFVCTGKPVASAWLH 240

Query: 241 KSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKML 300
           KSILK SLD++GRLMVD YLRVKG+HNIFAIGDITDIRESKQGESAKRQAKVAAKN+K L
Sbjct: 241 KSILKSSLDSHGRLMVDGYLRVKGRHNIFAIGDITDIRESKQGESAKRQAKVAAKNMKRL 300

Query: 301 MVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLG 360
           MVGK+SKM+ YVA++P TAMVSLG+KQA+AQFS TT+SGI PAFIKSRDLFVG  RK LG
Sbjct: 301 MVGKESKMESYVAQSPPTAMVSLGKKQAIAQFSFTTMSGIFPAFIKSRDLFVGKARKHLG 360

Query: 361 LHPTLLD 365
           LHPTLLD
Sbjct: 361 LHPTLLD 367

BLAST of Chy12G212540 vs. NCBI nr
Match: KAG6573579.1 (Apoptosis-inducing factor-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560 bits (1444), Expect = 1.69e-198
Identity = 283/365 (77.53%), Postives = 318/365 (87.12%), Query Frame = 0

Query: 1   MDVHGGGNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAE 60
           MD HGGG LRRV VVGGGIAGSL+AKSLQFFA+ITLIDPKEYLEIPYGSLRS VEP FAE
Sbjct: 1   MDEHGGGGLRRVVVVGGGIAGSLLAKSLQFFANITLIDPKEYLEIPYGSLRSTVEPSFAE 60

Query: 61  RMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRL 120
           R LI HS YFSNGRLITSPAVD+TESQVLVADGRHVDFDF+VVATGH  P LP TR  RL
Sbjct: 61  RSLIKHSEYFSNGRLITSPAVDITESQVLVADGRHVDFDFLVVATGHDHP-LPTTRALRL 120

Query: 121 HQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASE 180
             Y  ENERIR A SIL+IGGGP+G+ELAGEI T+F DKAITLVH+GPR+LEFMGPKAS+
Sbjct: 121 QHYREENERIREANSILVIGGGPSGVELAGEIATDFRDKAITLVHNGPRLLEFMGPKASD 180

Query: 181 KALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKS 240
           KAL+WLIS+RV VKL+Q ID+N++ +G K+FRSSKGE IRADCHFVCTG+ V SAWL K+
Sbjct: 181 KALKWLISRRVAVKLDQKIDLNNVSEGTKTFRSSKGETIRADCHFVCTGQPVGSAWLHKT 240

Query: 241 ILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMV 300
            LK +LD +GRLMVD  LRVKG++N+FAIGDITDIRESKQGESAKRQA+VAAKN+KM+MV
Sbjct: 241 TLKTNLDRHGRLMVDGNLRVKGRNNVFAIGDITDIRESKQGESAKRQARVAAKNMKMIMV 300

Query: 301 GKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLH 360
           GK+SKM+ Y   + TTA+VSLGRKQAVAQFS TTI+G LP FIKSRDLFVG  RK LGLH
Sbjct: 301 GKESKMEIYRPPSSTTAIVSLGRKQAVAQFSFTTIAGTLPGFIKSRDLFVGKARKHLGLH 360

Query: 361 PTLLD 365
           PT +D
Sbjct: 361 PTFVD 364

BLAST of Chy12G212540 vs. NCBI nr
Match: XP_022967089.1 (apoptosis-inducing factor 2-like [Cucurbita maxima])

HSP 1 Score: 560 bits (1442), Expect = 3.95e-198
Identity = 283/369 (76.69%), Postives = 320/369 (86.72%), Query Frame = 0

Query: 1   MDVHGGGN----LRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEP 60
           MD HGGG     LRRV VVGGGIAGSL+AKSLQFFA++TLIDPKEYLEIPYGSLRS VEP
Sbjct: 1   MDEHGGGGCGGGLRRVVVVGGGIAGSLLAKSLQFFANVTLIDPKEYLEIPYGSLRSTVEP 60

Query: 61  CFAERMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITR 120
            FAER LI HS YFSNGRLITSPAVD+TESQVLVADGRHVDFDF+VVATGH  P LP TR
Sbjct: 61  SFAERSLIKHSEYFSNGRLITSPAVDITESQVLVADGRHVDFDFLVVATGHDHP-LPTTR 120

Query: 121 THRLHQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGP 180
            HRL  Y  ENERIR A SILI+GGGP+G+ELAGEI T+F DKAITLVHDGPR+LEFMGP
Sbjct: 121 DHRLQHYREENERIREANSILIVGGGPSGVELAGEIVTDFRDKAITLVHDGPRLLEFMGP 180

Query: 181 KASEKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAW 240
           KAS+KAL+WL+S+RV VKL+Q ID+N++ +G K+F SSKGE IRADCHFVCTG+ V SAW
Sbjct: 181 KASDKALKWLVSRRVAVKLDQKIDLNNVSEGTKTFGSSKGETIRADCHFVCTGQPVGSAW 240

Query: 241 LRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLK 300
           L K++LK +LD +GRLMVD  LRVKG++N+FAIGDITDIRESKQGESAKRQAKVAAKN+K
Sbjct: 241 LHKTMLKINLDRHGRLMVDGTLRVKGRNNVFAIGDITDIRESKQGESAKRQAKVAAKNMK 300

Query: 301 MLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQ 360
           M+MVGK+SKM+ Y   + TTA+VSLGRKQAVAQFS+TTI+G LP FIKSRDLFVG  RK 
Sbjct: 301 MIMVGKESKMESYRPPSSTTAIVSLGRKQAVAQFSLTTIAGTLPGFIKSRDLFVGKARKH 360

Query: 361 LGLHPTLLD 365
           LGLHPT +D
Sbjct: 361 LGLHPTFVD 368

BLAST of Chy12G212540 vs. NCBI nr
Match: XP_023520994.1 (apoptosis-inducing factor 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557 bits (1436), Expect = 3.23e-197
Identity = 284/369 (76.96%), Postives = 319/369 (86.45%), Query Frame = 0

Query: 1   MDVHGGGN----LRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEP 60
           MD HGGG     LRRV VVGGGIAGSL+AKSLQFFA++TLIDPKEYLEIPYGSLRS VEP
Sbjct: 1   MDKHGGGGGGGGLRRVVVVGGGIAGSLLAKSLQFFANVTLIDPKEYLEIPYGSLRSTVEP 60

Query: 61  CFAERMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITR 120
            FAER LI HS YFSNGRLITSPAVD+TESQVLVA+GRHVDFDF+VVATGH  P LP TR
Sbjct: 61  SFAERSLIKHSEYFSNGRLITSPAVDITESQVLVANGRHVDFDFLVVATGHDHP-LPTTR 120

Query: 121 THRLHQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGP 180
             RL  Y  ENERIR A SILIIGGGP+G+ELAGEI T+F DKAITLVHDGPR+LEFMGP
Sbjct: 121 DLRLQHYRTENERIREANSILIIGGGPSGVELAGEIATDFRDKAITLVHDGPRLLEFMGP 180

Query: 181 KASEKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAW 240
           KAS+KAL+WLIS+RV VKL+Q ID+N++ +G K+FRSSKGE IRADCHFVCTGK V SAW
Sbjct: 181 KASDKALKWLISRRVAVKLDQKIDLNNVSEGTKTFRSSKGETIRADCHFVCTGKPVGSAW 240

Query: 241 LRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLK 300
           L K++LK +LD +GRLMVD  LRVKG+HNIFAIGDIT+IRESKQGESAKRQA+VAAKN+K
Sbjct: 241 LHKTMLKTNLDRHGRLMVDGNLRVKGRHNIFAIGDITNIRESKQGESAKRQARVAAKNMK 300

Query: 301 MLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQ 360
           M+MVGK+ KM+ Y   + TTA+VSLGRKQAVAQFS+TTI+G LP FIKSRDLFVG  RK 
Sbjct: 301 MIMVGKEGKMEIYRPPSSTTAIVSLGRKQAVAQFSLTTITGTLPGFIKSRDLFVGKARKH 360

Query: 361 LGLHPTLLD 365
           LGLHPT +D
Sbjct: 361 LGLHPTFVD 368

BLAST of Chy12G212540 vs. TAIR 10
Match: AT3G44190.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 422.9 bits (1086), Expect = 2.6e-118
Identity = 205/359 (57.10%), Postives = 271/359 (75.49%), Query Frame = 0

Query: 7   GNLRRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAERMLINH 66
           G  +RV V+GGGIAGSL +K LQF + +TLIDPKEY EI + SLRSMVEP FAER +INH
Sbjct: 9   GKGKRVIVIGGGIAGSLASKFLQFDSDLTLIDPKEYFEIIWASLRSMVEPAFAERSVINH 68

Query: 67  SHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRLHQYTAE 126
             Y  NGR++TSPAV++T++ VL ADG    +D++V+ATGH+D  LP TR  +L QY  E
Sbjct: 69  KKYLQNGRVVTSPAVNITDTDVLTADGLVFGYDYLVIATGHND-VLPKTRQEKLSQYQTE 128

Query: 127 NERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASEKALRWL 186
           +E+I+ +ESILI+GGGP+G+ELA EI  +FP+K +T+VH+GPR+LEF+G KA++KAL W+
Sbjct: 129 HEKIKSSESILIVGGGPSGVELAAEIAVDFPEKKVTIVHNGPRLLEFVGQKAADKALDWM 188

Query: 187 ISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKSILKKSL 246
            SKRV V L Q +D++   DG+K++R+S GE I ADCHF+CTGK ++S WL+ ++LK +L
Sbjct: 189 KSKRVEVILNQRVDLSSASDGSKTYRTSGGETIHADCHFLCTGKPLSSEWLKGTVLKDNL 248

Query: 247 DTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMVGKDSKM 306
           D  GRLMVD YLRVKG+ N+FAIGDITD+ E KQG  A++ A VA KN+K+LM G + K 
Sbjct: 249 DGKGRLMVDEYLRVKGRKNVFAIGDITDVPEMKQGYIAEKHASVATKNIKLLMSGGNEKK 308

Query: 307 KRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLHPTLLD 366
                  P  A++SLGRK +VAQF   T+SG +P  IKS+DLFVG TRK  GL P L++
Sbjct: 309 MSTYKPGPDIAIISLGRKDSVAQFPFMTVSGCIPGLIKSKDLFVGKTRKARGLDPNLVE 366

BLAST of Chy12G212540 vs. TAIR 10
Match: AT5G22140.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.0e-111
Identity = 196/355 (55.21%), Postives = 257/355 (72.39%), Query Frame = 0

Query: 10  RRVAVVGGGIAGSLVAKSLQFFAHITLIDPKEYLEIPYGSLRSMVEPCFAERMLINHSHY 69
           +RV V+GGGIAGSL AK LQF A +TLIDPKEY EI + SLRSMVEP FAER +INH  Y
Sbjct: 12  KRVVVIGGGIAGSLAAKLLQFDAEVTLIDPKEYFEITWASLRSMVEPKFAERTVINHKSY 71

Query: 70  FSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSLPITRTHRLHQYTAENER 129
              GRL+TSPA+++TES V+  DG  + +D++V+ATGH+D   P TR  +L  Y +E E+
Sbjct: 72  LKQGRLVTSPAINITESDVMTEDGSVIGYDYLVIATGHND-LFPKTRQEKLSHYQSEYEK 131

Query: 130 IRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLEFMGPKASEKALRWLISK 189
           I+ + S+LI+GGGP+G+ELA EI  +FP+K +TLVH GPR+LEF+G KA++KA  WL SK
Sbjct: 132 IKSSGSVLIVGGGPSGVELAAEIAVDFPEKKVTLVHKGPRLLEFVGQKAADKASDWLESK 191

Query: 190 RVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKSILKKSLDTN 249
           +V V L Q++D++   DGNK +R+S GE I AD HF+C GK ++S WL  ++LK SLD  
Sbjct: 192 KVEVILNQSVDLSSASDGNKIYRTSGGETIHADIHFLCVGKPLSSQWLNGTVLKDSLDGK 251

Query: 250 GRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAAKNLKMLMVGKDSKMKRY 309
           GR+MVD YLR++G+ N+FA+GDIT+I E KQG  A+  A V  KN+K++M G   K    
Sbjct: 252 GRVMVDEYLRIRGRSNVFAVGDITNIPEMKQGYIAETHANVVVKNIKVMMSGGKKKKMST 311

Query: 310 VARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGNTRKQLGLHPTLL 365
               P  A+VSLGRK +VAQF   T+ G LP  IKS+DLFVG TRK  GL+P L+
Sbjct: 312 YKPGPELAIVSLGRKDSVAQFPFVTVVGCLPGLIKSKDLFVGKTRKARGLNPKLV 365

BLAST of Chy12G212540 vs. TAIR 10
Match: AT5G22140.2 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 341.3 bits (874), Expect = 9.8e-94
Identity = 165/312 (52.88%), Postives = 222/312 (71.15%), Query Frame = 0

Query: 53  MVEPCFAERMLINHSHYFSNGRLITSPAVDVTESQVLVADGRHVDFDFVVVATGHHDPSL 112
           MVEP FAER +INH  Y   GRL+TSPA+++TES V+  DG  + +D++V+ATGH+D   
Sbjct: 1   MVEPKFAERTVINHKSYLKQGRLVTSPAINITESDVMTEDGSVIGYDYLVIATGHND-LF 60

Query: 113 PITRTHRLHQYTAENERIRRAESILIIGGGPTGIELAGEITTEFPDKAITLVHDGPRVLE 172
           P TR  +L  Y +E E+I+ + S+LI+GGGP+G+ELA EI  +FP+K +TLVH GPR+LE
Sbjct: 61  PKTRQEKLSHYQSEYEKIKSSGSVLIVGGGPSGVELAAEIAVDFPEKKVTLVHKGPRLLE 120

Query: 173 FMGPKASEKALRWLISKRVNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAV 232
           F+G KA++KA  WL SK+V V L Q++D++   DGNK +R+S GE I AD HF+C GK +
Sbjct: 121 FVGQKAADKASDWLESKKVEVILNQSVDLSSASDGNKIYRTSGGETIHADIHFLCVGKPL 180

Query: 233 ASAWLRKSILKKSLDTNGRLMVDAYLRVKGQHNIFAIGDITDIRESKQGESAKRQAKVAA 292
           +S WL  ++LK SLD  GR+MVD YLR++G+ N+FA+GDIT+I E KQG  A+  A V  
Sbjct: 181 SSQWLNGTVLKDSLDGKGRVMVDEYLRIRGRSNVFAVGDITNIPEMKQGYIAETHANVVV 240

Query: 293 KNLKMLMVGKDSKMKRYVARTPTTAMVSLGRKQAVAQFSITTISGILPAFIKSRDLFVGN 352
           KN+K++M G   K        P  A+VSLGRK +VAQF   T+ G LP  IKS+DLFVG 
Sbjct: 241 KNIKVMMSGGKKKKMSTYKPGPELAIVSLGRKDSVAQFPFVTVVGCLPGLIKSKDLFVGK 300

Query: 353 TRKQLGLHPTLL 365
           TRK  GL+P L+
Sbjct: 301 TRKARGLNPKLV 311

BLAST of Chy12G212540 vs. TAIR 10
Match: AT4G21490.1 (NAD(P)H dehydrogenase B3 )

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-11
Identity = 83/339 (24.48%), Postives = 137/339 (40.41%), Query Frame = 0

Query: 10  RRVAVVGGGIAGSLVAKSLQFFAH-ITLIDPKEYL-------EIPYGSL--RSMVEPC-- 69
           R+V ++G G AG+   K+L   ++ + +I P+ Y         +  G++  RS+VEP   
Sbjct: 56  RKVVLLGTGWAGASFLKTLNNSSYEVQVISPRNYFAFTPLLPSVTCGTVEARSVVEPIRN 115

Query: 70  FAERMLINHSHYFSNGRLIT--SPAVDVTESQVLVADGR---HVDFDFVVVATGHHDPSL 129
            A +  +  S   +    I   S  V     Q + + G+    VD+D++V+ATG    + 
Sbjct: 116 IARKQNVEMSFLEAECFKIDPGSKKVYCRSKQGVNSKGKKEFDVDYDYLVIATGAQSNTF 175

Query: 130 PITRTHRLHQYTAENERIRRAES------------------------ILIIGGGPTGIEL 189
            I        +  E E  +R  S                         +++GGGPTG+E 
Sbjct: 176 NIPGVEENCHFLKEVEDAQRIRSTVIDSFEKASLPGLNEQERKRMLHFVVVGGGPTGVEF 235

Query: 190 AGEI-------TTEFPDKA-----ITLVHDGPRVLEFMGPKASEKALRWLISKRVNVKL- 249
           A E+         +   KA     ITL+     +L     + +E A        ++VKL 
Sbjct: 236 ASELHDFVNEDLVKLYPKAKNLVQITLLEAADHILTMFDKRITEFAEEKFTRDGIDVKLG 295

Query: 250 EQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKSILKKSLDTNGR-LMV 294
              + VND      S ++  GE+       +     + +  + K  +K+    N R L  
Sbjct: 296 SMVVKVNDK---EISAKTKAGEVSTIPYGMIVWSTGIGTRPVIKDFMKQIGQGNRRALAT 355

BLAST of Chy12G212540 vs. TAIR 10
Match: AT4G05020.1 (NAD(P)H dehydrogenase B2 )

HSP 1 Score: 66.2 bits (160), Expect = 6.1e-11
Identity = 79/330 (23.94%), Postives = 137/330 (41.52%), Query Frame = 0

Query: 3   VHGGGNLRRVAVVGGGIAGSLVAKSLQFFAH-ITLIDPKEYL-------EIPYGSL--RS 62
           V  G   ++V ++G G AG+   K+L    + + +I P+ Y         +  G++  RS
Sbjct: 52  VETGTKKKKVVLLGTGWAGTSFLKNLNNSQYEVQIISPRNYFAFTPLLPSVTCGTVEARS 111

Query: 63  MVEPC-FAERMLINHSHYFSNGRLI--TSPAVDVTESQVLVADGR---HVDFDFVVVATG 122
           +VEP     R  ++ S+  +    I   S  V     Q L ++G+    VD+D++V+ATG
Sbjct: 112 VVEPIRNIGRKNVDTSYLEAECFKIDPASKKVYCRSKQGLSSNGKKEFSVDYDYLVIATG 171

Query: 123 HHDPSLPITRTHRLHQYTAE------------------------NERIRRAESILIIGGG 182
               +  I        +  E                        +E  +R    +++GGG
Sbjct: 172 AQSNTFNIPGVEENCHFLKEVEDAQRIRKTVIDSFEKASLPELSDEERKRILHFVVVGGG 231

Query: 183 PTGIELAGEI---TTE-----FP----DKAITLVHDGPRVLEFMGPKASEKALRWLISKR 242
           PTG+E A E+    TE     +P       ITL+     +L     + +E A        
Sbjct: 232 PTGVEFAAELHDFVTEDLVSLYPRAKGSVRITLLEAADHILTMFDKRITEFAEEKFSRDG 291

Query: 243 VNVKLEQTIDVNDMWDGNKSFRSSKGEIIRADCHFVCTGKAVASAWLRKSILKKSLDTNG 280
           ++VKL     V  + + + S ++  GE+       +     + +  + K  +K+    N 
Sbjct: 292 IDVKLGSM--VTKVNEKDISAKTKGGEVSSIPYGMIVWSTGIGTRPVIKDFMKQIGQGNR 351