Chy12G211010 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G211010
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Dimeric phloem specific lectin PP2
Location	chrH12: 6791762 .. 6792679 (+)
RNA-Seq Expression	Chy12G211010
Synteny	Chy12G211010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTACGGAAGCGGAAGCAAAACAGCATTTGGTAAAAAAACTTGAGTATGACCATTGCTTAGAAACAATTTTAGCCCTCGCAGACGACAAAGCTGGAGTCCAGTGGCCAACCTTCCTTCCTCTTTGCAATCAACTATCTCAACATGGCATCTCCCTTTGCAATGGAACTATCGTAAGTGTGTGCTTAAATTGAATATATTAATTTATTGTATAATATAATATGCAAATAAATATATATATACATTATTGAGTTTTGATTAATTAATTTTGTTGAATGCTTTCAGAAATTCAGAATTAATCTCGAAACAAACACTAATCGACTGTTCATATTACCAAAAATTCTCACTATTTTCGGGGTTAATGATCCCCGATACTGGAAATGGATCTACATCACGGACCATATGTACGTTTTTTTCTTTGTTAATTATTTCTAGAACCGAAATTCAATTTAATAATATTGTTATATATATATATATATATATATATTAATAAAAACAAAACGTGCAGTACTTCTGTGGATGCCCCTCAACTGATTGCAATACAAAAGTTAGACATACGTGGAATATTTCGTGCATCATTGATGACAACAAACGTCTTATACATTGTATCATTATTGGTAATGAAAACTGAGGATACCGCCGGATGGCAGACTCCTTTGAACGTTACAGTCACCACACCAGATGGGTCAACGAGACAGAGCCAAGTAAGCCTCGCGAGGAAGCCTATAGGCATTTTCTTTGAACTCACAGCAGGCGAGTTCACTCTCAGTGATGATGGATGTGACAATACCGGCCTAGTCAAGTTCACCGTAACGGAATACTCAAACTATGAAAAACGTGGGCTGCTGGTCAAAGGTTGCATTGTTCGAGCAAAGTTTTCACTTGGCTGCACAAATCCAACTTTGAACCGTGCATAA

mRNA sequence

ATGACTACGGAAGCGGAAGCAAAACAGCATTTGGTAAAAAAACTTGAGTATGACCATTGCTTAGAAACAATTTTAGCCCTCGCAGACGACAAAGCTGGAGTCCAGTGGCCAACCTTCCTTCCTCTTTGCAATCAACTATCTCAACATGGCATCTCCCTTTGCAATGGAACTATCAAATTCAGAATTAATCTCGAAACAAACACTAATCGACTGTTCATATTACCAAAAATTCTCACTATTTTCGGGGTTAATGATCCCCGATACTGGAAATGGATCTACATCACGGACCATATTACTTCTGTGGATGCCCCTCAACTGATTGCAATACAAAAGTTAGACATACGTGGAATATTTCGTGCATCATTGATGACAACAAACGTCTTATACATTGTATCATTATTGGTAATGAAAACTGAGGATACCGCCGGATGGCAGACTCCTTTGAACGTTACAGTCACCACACCAGATGGGTCAACGAGACAGAGCCAAGTAAGCCTCGCGAGGAAGCCTATAGGCATTTTCTTTGAACTCACAGCAGGCGAGTTCACTCTCAGTGATGATGGATGTGACAATACCGGCCTAGTCAAGTTCACCGTAACGGAATACTCAAACTATGAAAAACGTGGGCTGCTGGTCAAAGGTTGCATTGTTCGAGCAAAGTTTTCACTTGGCTGCACAAATCCAACTTTGAACCGTGCATAA

Coding sequence (CDS)

ATGACTACGGAAGCGGAAGCAAAACAGCATTTGGTAAAAAAACTTGAGTATGACCATTGCTTAGAAACAATTTTAGCCCTCGCAGACGACAAAGCTGGAGTCCAGTGGCCAACCTTCCTTCCTCTTTGCAATCAACTATCTCAACATGGCATCTCCCTTTGCAATGGAACTATCAAATTCAGAATTAATCTCGAAACAAACACTAATCGACTGTTCATATTACCAAAAATTCTCACTATTTTCGGGGTTAATGATCCCCGATACTGGAAATGGATCTACATCACGGACCATATTACTTCTGTGGATGCCCCTCAACTGATTGCAATACAAAAGTTAGACATACGTGGAATATTTCGTGCATCATTGATGACAACAAACGTCTTATACATTGTATCATTATTGGTAATGAAAACTGAGGATACCGCCGGATGGCAGACTCCTTTGAACGTTACAGTCACCACACCAGATGGGTCAACGAGACAGAGCCAAGTAAGCCTCGCGAGGAAGCCTATAGGCATTTTCTTTGAACTCACAGCAGGCGAGTTCACTCTCAGTGATGATGGATGTGACAATACCGGCCTAGTCAAGTTCACCGTAACGGAATACTCAAACTATGAAAAACGTGGGCTGCTGGTCAAAGGTTGCATTGTTCGAGCAAAGTTTTCACTTGGCTGCACAAATCCAACTTTGAACCGTGCATAA

Protein sequence

MTTEAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRASLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAGEFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTLNRA*

Homology

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BLAST of Chy12G211010 vs. ExPASy Swiss-Prot
Match: C0HJV2 (Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.6e-23
Identity = 66/208 (31.73%), Postives = 116/208 (55.77%), Query Frame = 0


Query: 14  KLEYDHCLETILALAD-DKAGVQWPTFLPLCNQLSQHGISLCNGTIKFRINLETNTNRLF 73
           +++  H LE IL   D D   V  P+F+ L +Q++  GI L N T ++  +    +N   
Sbjct: 7   EVKVGHNLEAILKGLDVDVYSV--PSFIKLYDQVTA-GIFLNNRTKRYWFDKNAESNCFM 66

Query: 74  ILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRASLMTTNVLYIVS 133
           +  + L I    D RYW+W    +H  +++  +LI +  L+I G    S+++  + Y  +
Sbjct: 67  LYARDLLITWSQDKRYWRWNPFQEHGNTLEVAELIDVCWLNIVGNIETSVLSPGISYEAA 126

Query: 134 LLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAGEFTLSDDGCDNT 193
             VM T   +GW+ P++V +  PDGS ++SQV+L  KP G++F ++ G F +S    +  
Sbjct: 127 FEVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDKPRGVWFFISVGHFKISVG--ETI 186

Query: 194 GLVKFTVTEYSNYEKRGLLVKGCIVRAK 221
           G ++F++ ++    KRGLLVKG +++ K
Sbjct: 187 GNIEFSIVQHQE-AKRGLLVKGLVIQPK 208

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BLAST of Chy12G211010 vs. ExPASy Swiss-Prot
Match: Q9C8U9 (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702 GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.5e-12
Identity = 58/155 (37.42%), Postives = 82/155 (52.90%), Query Frame = 0


Query: 73  ILPKILTIFGVNDPRYWKWIYITDHITS---VDAPQLIAIQKLDIRGIFRASLMTTNVLY 132
           I  + L+I   +   YW W+ +   I+S   VDA  L A+  LD+ G F    +T    Y
Sbjct: 14  IYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLETTY 73

Query: 133 IVSLLVMKTEDTA-GWQTPLNVTVTTPDGSTRQSQVSLARKP-IG-IFFELTAGEFTLSD 192
            V + V+K EDTA GW  P+N+ +T PDG  R  + S+  K  IG  + +++AGEF  S 
Sbjct: 74  EV-VYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTSP 133

Query: 193 DGCDNTGLVKFTVTE-YSNYEKRGLLVKGCIVRAK 221
              DN G + F++ E  S   KRGL VK   +R K
Sbjct: 134 ---DNAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

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BLAST of Chy12G211010 vs. ExPASy Swiss-Prot
Match: P0DSP5 (Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-11
Identity = 44/149 (29.53%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 62  INLETNTNRLFIL-PKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRA 121
           +N E  ++  F+L P+  T+   +D RYW W  +      ++  QL  +   D R     
Sbjct: 1   LNQEKLSSTHFLLFPRAATLTWSDDTRYWSWNPVDFCGYQLEEAQLSRVSWFDCRWTVNT 60

Query: 122 SLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAG 181
           + + TNV Y V L V      +GW TPLN+ +  P+GS + SQV L  +P  ++F+L  G
Sbjct: 61  TDLKTNVWYNVFLKVQMGSGASGWNTPLNLELEMPNGSKQASQVVLNDRPRDVWFKLQMG 120

Query: 182 EFTLSDDGCDNTGLVKFTVTEYSNYEKRG 210
              +SD   +  G ++ ++  +    K G
Sbjct: 121 NLMVSDS--ETCGALRMSLYNHQTNWKMG 147

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BLAST of Chy12G211010 vs. ExPASy Swiss-Prot
Match: Q9ZVR1 (F-box protein PP2-B5 OS=Arabidopsis thaliana OX=3702 GN=PP2B5 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.7e-09
Identity = 48/190 (25.26%), Postives = 87/190 (45.79%), Query Frame = 0


Query: 39  FLPLCNQLSQHGISLCNGTIKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHI 98
           +  LC+    H     NG + FR++  +    + +  K L I  V +P+YWKWI I +  
Sbjct: 101 YFALCDPFPNH-----NGKMSFRLDKASGKKCVMLSAKKLLISRVVNPKYWKWISIPESR 160

Query: 99  TSVDAPQLIAIQKLDIRGIFRASLMTTNVLYIVSLLVMKTEDTAGWQT-PLNVTVTTPDG 158
              + P+L+ I   DIRG+    +++    Y   ++  KT    G+QT P+   V     
Sbjct: 161 FD-EVPELLNIDSFDIRGVLNTRIISPGTHYSAYIVYTKTSHFNGFQTSPIQAGVGFQRH 220

Query: 159 STRQSQVSL-ARKPIGIFFELTAGEFTLSDDGCDNTGLVKFTVTEYSNYE----KRGLLV 218
              ++ +   ++K    + E   G+F  ++ G     L++ +V + + Y     K GL++
Sbjct: 221 GMSKTFIRFDSKKRQDGWMEAKIGDF-YNEGGLIGFNLIEVSVVDVARYPHMNMKSGLII 280

Query: 219 KGCIVRAKFS 223
           +G   R K S
Sbjct: 281 EGIEFRPKDS 283

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BLAST of Chy12G211010 vs. ExPASy TrEMBL
Match: A0A0A0M183 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G703060 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.3e-118
Identity = 208/233 (89.27%), Postives = 223/233 (95.71%), Query Frame = 0


Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKF 60
           MTTEAEAKQHLVKKLEYDHCLETILALADD+AGV+WPTFLPLCNQLSQ GISLCNGTIKF
Sbjct: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRA 120
           RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRG F A
Sbjct: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120

Query: 121 SLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAG 180
            L+T  VL ++SLLV+ TE+T GWQTPLNVT+TTPDGSTRQSQVSLARKPIGIFFELT G
Sbjct: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180

Query: 181 EFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTLNRA 234
           EFTL+DDGC++TGLVKF+VTEYSNYEKRG+L+KGCIVRAKFSLGCTNPTL+RA
Sbjct: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA 233

[Close]

BLAST of Chy12G211010 vs. ExPASy TrEMBL
Match: A0A5D3CK31 (Dimeric phloem specific lectin PP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00020 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 7.5e-87
Identity = 156/230 (67.83%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKF 60
           M +E EAK+ LV KL Y++CLE+ILALADDKA ++ P FLPLC+QL+  GISLC GTIKF
Sbjct: 1   MGSEEEAKKLLVNKLVYNNCLESILALADDKAALKLPVFLPLCDQLAS-GISLCGGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRA 120
           R+N+ TNTNRLFILPKILTI+ VNDPRYW WI+ITDHIT+VD P+LI+I KLDIRG F +
Sbjct: 61  RLNIATNTNRLFILPKILTIYRVNDPRYWTWIHITDHITAVDGPKLISITKLDIRGKFSS 120

Query: 121 SLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAG 180
            L+T N +Y+ +LLV+  ED +GW+TP+NV++TTPDG T  +QVSL RKPIG+FFEL  G
Sbjct: 121 RLLTQNTVYVCALLVIFYEDKSGWETPVNVSLTTPDGKTVTNQVSLERKPIGMFFELPVG 180

Query: 181 EFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTL 231
           EFT+++ GC++TGLV+F VTEYSN+EKRGLL+KGC+VRAKFS GCT PT+
Sbjct: 181 EFTVNNKGCEDTGLVQFAVTEYSNHEKRGLLIKGCLVRAKFSNGCTTPTV 229

[Close]

BLAST of Chy12G211010 vs. ExPASy TrEMBL
Match: A0A1S3C9A4 (uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Cucumis melo OX=3656 GN=LOC103498272 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 7.5e-87
Identity = 156/230 (67.83%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKF 60
           M +E EAK+ LV KL Y++CLE+ILALADDKA ++ P FLPLC+QL+  GISLC GTIKF
Sbjct: 1   MGSEEEAKKLLVNKLVYNNCLESILALADDKAALKLPVFLPLCDQLAS-GISLCGGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRA 120
           R+N+ TNTNRLFILPKILTI+ VNDPRYW WI+ITDHIT+VD P+LI+I KLDIRG F +
Sbjct: 61  RLNIATNTNRLFILPKILTIYRVNDPRYWTWIHITDHITAVDGPKLISITKLDIRGKFSS 120

Query: 121 SLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAG 180
            L+T N +Y+ +LLV+  ED +GW+TP+NV++TTPDG T  +QVSL RKPIG+FFEL  G
Sbjct: 121 RLLTQNTVYVCALLVIFYEDKSGWETPVNVSLTTPDGKTVTNQVSLERKPIGMFFELPVG 180

Query: 181 EFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTL 231
           EFT+++ GC++TGLV+F VTEYSN+EKRGLL+KGC+VRAKFS GCT PT+
Sbjct: 181 EFTVNNKGCEDTGLVQFAVTEYSNHEKRGLLIKGCLVRAKFSNGCTTPTV 229

[Close]

BLAST of Chy12G211010 vs. ExPASy TrEMBL
Match: Q42383 (Dimeric phloem specific lectin PP2 OS=Cucurbita maxima OX=3661 GN=PP2 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 8.4e-30
Identity = 84/222 (37.84%), Postives = 124/222 (55.86%), Query Frame = 0


Query: 4   EAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKFRIN 63
           E EA++ L  +++  HCL+ IL  AD    + +P+FL L +QL   GI L  G IK+  +
Sbjct: 5   EKEAREKLGGEVKLGHCLDVILKNAD--VALHYPSFLKLYDQLVA-GILLNKGAIKYIFD 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRASLM 123
            ++N+N  FI  + L+I  + D RYWKW    D     +  +LI +  LDIRG    S++
Sbjct: 65  KKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDS----NVAELIEVSWLDIRGKINESML 124

Query: 124 TTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAGEFT 183
           + NV+Y V+L V      +GW  PLN+ +  PDGS    Q  L  KP   +FE+   EF 
Sbjct: 125 SQNVVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVV-EFK 184

Query: 184 LSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGC 226
           + + GC ++G ++F   E+  + KRGLLVKG  + AK   GC
Sbjct: 185 VGNHGCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAK-GCGC 217

[Close]

BLAST of Chy12G211010 vs. ExPASy TrEMBL
Match: Q9LLT3 (Phloem protein 2 OS=Cucurbita moschata OX=3662 GN=pp2 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 4.2e-29
Identity = 80/223 (35.87%), Postives = 126/223 (56.50%), Query Frame = 0


Query: 4   EAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKFRIN 63
           E EA++ L  +++  HCL+ IL  AD    + +P+F+ L +QL   GI L  G I++  +
Sbjct: 5   EREAREKLGGEVKLGHCLDVILKNAD--VALHYPSFVKLYDQLVA-GILLNKGAIRYIFD 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRASLM 123
            ++N+N  FI  + L+I  + D RYWKW       + +   +LI +  LDIRG    S++
Sbjct: 65  KKSNSNWYFIFARALSIAWIEDKRYWKW------GSCIKIAELIEVSWLDIRGKINESML 124

Query: 124 TTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAGEFT 183
           + N++Y V+L V   +  +GW  PLN+ +  PDGS    Q  L  KP   +FE+   EF 
Sbjct: 125 SPNIVYEVALQVQLNDRASGWDAPLNIELKKPDGSKIVRQECLLGKPRNQWFEIVV-EFK 184

Query: 184 LSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCT 227
           + + GC ++G ++F   E+  + KRGLLVKG  + AK   GC+
Sbjct: 185 VGNHGCGSSGEIEFAFYEHGGHWKRGLLVKGVRIGAK-GCGCS 216

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BLAST of Chy12G211010 vs. NCBI nr
Match: XP_004145448.1 (lectin [Cucumis sativus] >KGN66882.1 hypothetical protein Csa_007569 [Cucumis sativus])

HSP 1 Score: 429 bits (1104), Expect = 4.36e-151
Identity = 208/233 (89.27%), Postives = 223/233 (95.71%), Query Frame = 0


Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKF 60
           MTTEAEAKQHLVKKLEYDHCLETILALADD+AGV+WPTFLPLCNQLSQ GISLCNGTIKF
Sbjct: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDQAGVEWPTFLPLCNQLSQRGISLCNGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRA 120
           RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRG F A
Sbjct: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGAFSA 120

Query: 121 SLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAG 180
            L+T  VL ++SLLV+ TE+T GWQTPLNVT+TTPDGSTRQSQVSLARKPIGIFFELT G
Sbjct: 121 PLVTPKVLNVLSLLVLLTENTGGWQTPLNVTITTPDGSTRQSQVSLARKPIGIFFELTVG 180

Query: 181 EFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTLNRA 233
           EFTL+DDGC++TGLVKF+VTEYSNYEKRG+L+KGCIVRAKFSLGCTNPTL+RA
Sbjct: 181 EFTLNDDGCNSTGLVKFSVTEYSNYEKRGMLIKGCIVRAKFSLGCTNPTLDRA 233

[Close]

BLAST of Chy12G211010 vs. NCBI nr
Match: XP_008459050.1 (PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 [Cucumis melo] >KAA0043215.1 dimeric phloem specific lectin PP2 [Cucumis melo var. makuwa] >TYK12253.1 dimeric phloem specific lectin PP2 [Cucumis melo var. makuwa])

HSP 1 Score: 324 bits (831), Expect = 1.67e-109
Identity = 156/230 (67.83%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MTTEAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKF 60
           M +E EAK+ LV KL Y++CLE+ILALADDKA ++ P FLPLC+QL+  GISLC GTIKF
Sbjct: 1   MGSEEEAKKLLVNKLVYNNCLESILALADDKAALKLPVFLPLCDQLAS-GISLCGGTIKF 60

Query: 61  RINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRA 120
           R+N+ TNTNRLFILPKILTI+ VNDPRYW WI+ITDHIT+VD P+LI+I KLDIRG F +
Sbjct: 61  RLNIATNTNRLFILPKILTIYRVNDPRYWTWIHITDHITAVDGPKLISITKLDIRGKFSS 120

Query: 121 SLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAG 180
            L+T N +Y+ +LLV+  ED +GW+TP+NV++TTPDG T  +QVSL RKPIG+FFEL  G
Sbjct: 121 RLLTQNTVYVCALLVIFYEDKSGWETPVNVSLTTPDGKTVTNQVSLERKPIGMFFELPVG 180

Query: 181 EFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTL 230
           EFT+++ GC++TGLV+F VTEYSN+EKRGLL+KGC+VRAKFS GCT PT+
Sbjct: 181 EFTVNNKGCEDTGLVQFAVTEYSNHEKRGLLIKGCLVRAKFSNGCTTPTV 229

[Close]

BLAST of Chy12G211010 vs. NCBI nr
Match: XP_038893562.1 (lectin-like [Benincasa hispida])

HSP 1 Score: 215 bits (548), Expect = 4.59e-66
Identity = 111/228 (48.68%), Postives = 160/228 (70.18%), Query Frame = 0


Query: 4   EAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKFRIN 63
           E EA+++L  KL Y H L+ I + +DDK  +Q PTF+PL +QL   G+S   GT KFR+N
Sbjct: 5   ELEARKYLGVKLSYGHNLDCIFSHSDDK--LQNPTFVPLFDQLLD-GVSFNQGTRKFRLN 64

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRASLM 123
             TN+NR+FILPK L+I  VND RYWKWI +TD+   V+APQLI +   DI G  +AS +
Sbjct: 65  QPTNSNRVFILPKALSIAWVNDTRYWKWIIVTDNRNEVEAPQLIQVSWFDIHGSVKASWL 124

Query: 124 TTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAGEFT 183
           ++ V+Y+V+ LVM TED +GW   +N+T+  P+G T +++VSL  KP G FFE+ AG+F 
Sbjct: 125 SSRVVYLVAFLVMLTEDASGWNHHVNLTIKKPNGCTVETKVSLEGKPKGEFFEVWAGDFI 184

Query: 184 LSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTLN 231
           L + GC ++G+V+F + E+  + KRGL+VKG ++R+K S GC + TL+
Sbjct: 185 LDNCGCGDSGVVEFGMYEHGGHWKRGLIVKGVVIRSKASAGCPHETLS 229

[Close]

BLAST of Chy12G211010 vs. NCBI nr
Match: WP_219729244.1 (PP2 domain-containing protein, partial [Pectobacterium odoriferum] >POE05059.1 hypothetical protein BV921_23310, partial [Pectobacterium odoriferum])

HSP 1 Score: 181 bits (460), Expect = 3.26e-53
Identity = 91/227 (40.09%), Postives = 149/227 (65.64%), Query Frame = 0


Query: 4   EAEAKQHLVKKLEYDHCLETILALADDKAGVQWPTFLPLCNQLSQHGISLCNGTIKFRIN 63
           E EA+++L  +++  HCL  IL  AD+K  + WP+++PL +QL  +GISL NG  K+ +N
Sbjct: 10  EVEARKYLGLQVQLGHCLSYILPQADEK--LLWPSYIPLFDQLV-YGISLNNGAQKYYLN 69

Query: 64  LETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIFRASLM 123
            +TN+NR++I PK L I   +DPRYWKW  + +         LI +   D+RG  +ASL+
Sbjct: 70  KQTNSNRVYIPPKSLNIAWGHDPRYWKWELLEEWDKRALLAVLIKVNWFDVRGSVKASLL 129

Query: 124 TTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELTAGEFT 183
           ++ +LY+V+ +V   ED  GW  P+N+ +  P+G   +S+V++  KP G +FE+ AGE  
Sbjct: 130 SSRILYLVAFVVYFNEDAHGWHVPVNLVLKKPNGCKIESKVNIEGKPKGEYFEVDAGELI 189

Query: 184 LSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTL 230
           L + GC ++G+++F + E+ ++EKRGL++KG I+R+K S GC +  +
Sbjct: 190 LDNCGCGDSGIIEFAMYEHGSHEKRGLVMKGVILRSKASDGCPHANV 233

[Close]

BLAST of Chy12G211010 vs. NCBI nr
Match: WP_181002512.1 (PP2 domain-containing protein, partial [Pectobacterium odoriferum] >POE16080.1 hypothetical protein BV923_23560, partial [Pectobacterium odoriferum])

HSP 1 Score: 142 bits (357), Expect = 1.99e-38
Identity = 67/172 (38.95%), Postives = 112/172 (65.12%), Query Frame = 0


Query: 59  KFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHITSVDAPQLIAIQKLDIRGIF 118
           K+ +N +TN+NR++I PK L I   +DPRYWKW  + +         LI +   D+RG  
Sbjct: 6   KYYLNKQTNSNRVYIPPKSLNIAWGHDPRYWKWELLEEWDKRALLAVLIKVNWFDVRGSV 65

Query: 119 RASLMTTNVLYIVSLLVMKTEDTAGWQTPLNVTVTTPDGSTRQSQVSLARKPIGIFFELT 178
           +ASL+++ +LY+V+ +V   ED  GW  P+N+ +  P+G   +S+V++  KP G +FE+ 
Sbjct: 66  KASLLSSRILYLVAFVVYFNEDAHGWHVPVNLVLKKPNGCKIESKVNIEGKPKGEYFEVD 125

Query: 179 AGEFTLSDDGCDNTGLVKFTVTEYSNYEKRGLLVKGCIVRAKFSLGCTNPTL 230
           AGE  L + GC ++G+++F + E+ ++EKRGL++KG I+R+K S GC +  +
Sbjct: 126 AGELILDNCGCGDSGIIEFAMYEHGSHEKRGLVMKGVILRSKASDGCPHANV 177

[Close]

BLAST of Chy12G211010 vs. TAIR 10
Match: AT1G33920.1 (phloem protein 2-A4 )

HSP 1 Score: 73.2 bits (178), Expect = 3.2e-13
Identity = 58/155 (37.42%), Postives = 82/155 (52.90%), Query Frame = 0


Query: 73  ILPKILTIFGVNDPRYWKWIYITDHITS---VDAPQLIAIQKLDIRGIFRASLMTTNVLY 132
           I  + L+I   +   YW W+ +   I+S   VDA  L A+  LD+ G F    +T    Y
Sbjct: 14  IYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLETTY 73

Query: 133 IVSLLVMKTEDTA-GWQTPLNVTVTTPDGSTRQSQVSLARKP-IG-IFFELTAGEFTLSD 192
            V + V+K EDTA GW  P+N+ +T PDG  R  + S+  K  IG  + +++AGEF  S 
Sbjct: 74  EV-VYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTSP 133

Query: 193 DGCDNTGLVKFTVTE-YSNYEKRGLLVKGCIVRAK 221
              DN G + F++ E  S   KRGL VK   +R K
Sbjct: 134 ---DNAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

[Close]

BLAST of Chy12G211010 vs. TAIR 10
Match: AT2G02300.1 (phloem protein 2-B5 )

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-10
Identity = 48/190 (25.26%), Postives = 87/190 (45.79%), Query Frame = 0


Query: 39  FLPLCNQLSQHGISLCNGTIKFRINLETNTNRLFILPKILTIFGVNDPRYWKWIYITDHI 98
           +  LC+    H     NG + FR++  +    + +  K L I  V +P+YWKWI I +  
Sbjct: 101 YFALCDPFPNH-----NGKMSFRLDKASGKKCVMLSAKKLLISRVVNPKYWKWISIPESR 160

Query: 99  TSVDAPQLIAIQKLDIRGIFRASLMTTNVLYIVSLLVMKTEDTAGWQT-PLNVTVTTPDG 158
              + P+L+ I   DIRG+    +++    Y   ++  KT    G+QT P+   V     
Sbjct: 161 FD-EVPELLNIDSFDIRGVLNTRIISPGTHYSAYIVYTKTSHFNGFQTSPIQAGVGFQRH 220

Query: 159 STRQSQVSL-ARKPIGIFFELTAGEFTLSDDGCDNTGLVKFTVTEYSNYE----KRGLLV 218
              ++ +   ++K    + E   G+F  ++ G     L++ +V + + Y     K GL++
Sbjct: 221 GMSKTFIRFDSKKRQDGWMEAKIGDF-YNEGGLIGFNLIEVSVVDVARYPHMNMKSGLII 280

Query: 219 KGCIVRAKFS 223
           +G   R K S
Sbjct: 281 EGIEFRPKDS 283

The following BLAST results are available for this feature:

BLAST of Chy12G211010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HJV2	5.6e-23	31.73	Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1	[more]
Q9C8U9	4.5e-12	37.42	Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702...	[more]
P0DSP5	1.7e-11	29.53	Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1	[more]
Q9ZVR1	2.7e-09	25.26	F-box protein PP2-B5 OS=Arabidopsis thaliana OX=3702 GN=PP2B5 PE=2 SV=1	[more]

BLAST of Chy12G211010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M183	1.3e-118	89.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G703060 PE=4 SV=1	[more]
A0A5D3CK31	7.5e-87	67.83	Dimeric phloem specific lectin PP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C9A4	7.5e-87	67.83	uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
Q42383	8.4e-30	37.84	Dimeric phloem specific lectin PP2 OS=Cucurbita maxima OX=3661 GN=PP2 PE=2 SV=1	[more]
Q9LLT3	4.2e-29	35.87	Phloem protein 2 OS=Cucurbita moschata OX=3662 GN=pp2 PE=2 SV=1	[more]

BLAST of Chy12G211010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145448.1	4.36e-151	89.27	lectin [Cucumis sativus] >KGN66882.1 hypothetical protein Csa_007569 [Cucumis sa...	[more]
XP_008459050.1	1.67e-109	67.83	PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 [Cucumis melo] >KAA0...	[more]
XP_038893562.1	4.59e-66	48.68	lectin-like [Benincasa hispida]	[more]
WP_219729244.1	3.26e-53	40.09	PP2 domain-containing protein, partial [Pectobacterium odoriferum] >POE05059.1 h...	[more]
WP_181002512.1	1.99e-38	38.95	PP2 domain-containing protein, partial [Pectobacterium odoriferum] >POE16080.1 h...	[more]

BLAST of Chy12G211010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G33920.1	3.2e-13	37.42	phloem protein 2-A4	[more]
AT2G02300.1	1.9e-10	25.26	phloem protein 2-B5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025886	Phloem protein 2-like	PFAM	PF14299	PP2	coord: 72..218 e-value: 3.0E-18 score: 66.3
None	No IPR available	PANTHER	PTHR32278	FAMILY NOT NAMED	coord: 33..218
None	No IPR available	PANTHER	PTHR32278:SF42	PROTEIN PHLOEM PROTEIN 2-LIKE A2	coord: 33..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G211010.1	Chy12G211010.1	mRNA