Chy12G210880 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G210880
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	E3 ubiquitin-protein ligase BOI-like
Location	chrH12: 6400491 .. 6401588 (+)
RNA-Seq Expression	Chy12G210880
Synteny	Chy12G210880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGTTGAAGCACGGCCTATGAATCTTCCACCTTCTCATCAGTTCATCATCCCCAACAACAGGTCCATAATCTCAATCCATATATTTATATGTATATATATATATCTTCCTTTTCATTTTCTTTTTCAATTCTTTCCTCTCACACACAACTCTCTTTTATTTTCTCAGAGATTTCATCAAACAGAATCAAGAATTAGCAAACACCCACATGACACCTGCCACGGACACCACCACCCCAGGAATAATATGGCCTTACACGGCCGTCAAACCAGCGGTTACACCGGAGACGACTCTCAATAATTTCGCCACTATGTACGATTGGGGAAAAGCAGAGAGTGGACTTACATCCAATAACTTCCCATCAACCGCTCCCACCCGAAAGCGAACGAGATCGCGGTCCTTGTACAATGAACCAGGGGGGAGGTTGTTGGATGAAGAAATCATAAACTCCCACATTCAGCAACAACAGAGTGAAATAGATCGGTTCATTGCAATCCATGTAAGTCCCAACTTTTTTTAATTACCTTCTGAGAGTGGGTTTAAAATTAAACGATGTACACTTGATGAAACTGACATCGGTTTTAAATTACAGAGAGAGAAGATGAGGATTGAAATGGAGATGCGGAAGAAGAGGGAATCGGGGATGTTGGTAAGGGCAATAGAGGAAAAAGTGGTAAAGAAGTTGAAGGAAAAAGAAGAAGAAATAGAAAGAATGGGGAAATTGAATTGGGTATTGCAAGAGAGAGTGAAACGGCTGTGTGTGGAGAATCAAGTATGGAGGGATTTGGCAGAGAGCAATGAAGCGACGGTGAATTGTTTAAGGAATAATTTGGAGCAGGTGATATTAATGGCGGCAAATAAGAACGTGGGTGGTGTGGCAGGGGCGAAGGAGAAGGAGGAGAAGGCGGAGTCAAGCTGTGGGAGTACAAGTGAGTGTGGAAGGAAGGAGGTGGAGGAGGAGGAGGCGGAGAGTGGGGGAGGAGGGCGGTGCCGGAAGTGTGGGGCGGGGGAATCGAGGGTGTTGGTGTTGCCATGCAGGCATCTTTGTTTGTGTACAATGTGTGGGTCCACGATTCACAGTTGCCCAGTATGTAA

mRNA sequence

ATGGCAGTTGAAGCACGGCCTATGAATCTTCCACCTTCTCATCAGTTCATCATCCCCAACAACAGAGATTTCATCAAACAGAATCAAGAATTAGCAAACACCCACATGACACCTGCCACGGACACCACCACCCCAGGAATAATATGGCCTTACACGGCCGTCAAACCAGCGGTTACACCGGAGACGACTCTCAATAATTTCGCCACTATGTACGATTGGGGAAAAGCAGAGAGTGGACTTACATCCAATAACTTCCCATCAACCGCTCCCACCCGAAAGCGAACGAGATCGCGGTCCTTGTACAATGAACCAGGGGGGAGGTTGTTGGATGAAGAAATCATAAACTCCCACATTCAGCAACAACAGAGTGAAATAGATCGGTTCATTGCAATCCATAGAGAGAAGATGAGGATTGAAATGGAGATGCGGAAGAAGAGGGAATCGGGGATGTTGGTAAGGGCAATAGAGGAAAAAGTGGTAAAGAAGTTGAAGGAAAAAGAAGAAGAAATAGAAAGAATGGGGAAATTGAATTGGGTATTGCAAGAGAGAGTGAAACGGCTGTGTGTGGAGAATCAAGTATGGAGGGATTTGGCAGAGAGCAATGAAGCGACGGTGAATTGTTTAAGGAATAATTTGGAGCAGGCATCTTTGTTTGTGTACAATGTGTGGGTCCACGATTCACAGTTGCCCAGTATGTAA

Coding sequence (CDS)

ATGGCAGTTGAAGCACGGCCTATGAATCTTCCACCTTCTCATCAGTTCATCATCCCCAACAACAGAGATTTCATCAAACAGAATCAAGAATTAGCAAACACCCACATGACACCTGCCACGGACACCACCACCCCAGGAATAATATGGCCTTACACGGCCGTCAAACCAGCGGTTACACCGGAGACGACTCTCAATAATTTCGCCACTATGTACGATTGGGGAAAAGCAGAGAGTGGACTTACATCCAATAACTTCCCATCAACCGCTCCCACCCGAAAGCGAACGAGATCGCGGTCCTTGTACAATGAACCAGGGGGGAGGTTGTTGGATGAAGAAATCATAAACTCCCACATTCAGCAACAACAGAGTGAAATAGATCGGTTCATTGCAATCCATAGAGAGAAGATGAGGATTGAAATGGAGATGCGGAAGAAGAGGGAATCGGGGATGTTGGTAAGGGCAATAGAGGAAAAAGTGGTAAAGAAGTTGAAGGAAAAAGAAGAAGAAATAGAAAGAATGGGGAAATTGAATTGGGTATTGCAAGAGAGAGTGAAACGGCTGTGTGTGGAGAATCAAGTATGGAGGGATTTGGCAGAGAGCAATGAAGCGACGGTGAATTGTTTAAGGAATAATTTGGAGCAGGCATCTTTGTTTGTGTACAATGTGTGGGTCCACGATTCACAGTTGCCCAGTATGTAA

Protein sequence

MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTPETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGGRLLDEEIINSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQASLFVYNVWVHDSQLPSM*

Homology

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BLAST of Chy12G210880 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.7e-30
Identity = 98/221 (44.34%), Postives = 131/221 (59.28%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MNL  S Q+I   NR+ +K     + T+M         G         P    
Sbjct: 1   MAVEARHMNL-FSSQYI--TNRECVK-----SQTNMNNGQQIAGGGF--------PVTIG 60

Query: 61  ETTLNNFATMYDWGKAESGLTS-------NNFPSTAPTRKRTRSRSLYNEPGGRLLDEEI 120
           +  L     +  + K+ES LT+       + F S A    + R R++   P   L+D E+
Sbjct: 61  DRNLQYIDPINSFNKSESELTAISKRQRDSTFDSDALIASQKR-RAIAFSPAS-LIDAEL 120

Query: 121 INSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERM 180
           + S IQQQ SEIDRF+A   E +RIE+E R++ ++ ML  A++  ++KKLK K+EEI RM
Sbjct: 121 V-SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRM 180

Query: 181 GKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           GKLNWVLQERVK L VENQ+WRDLA++NEAT N LR+NLEQ
Sbjct: 181 GKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQ 202

[Close]

BLAST of Chy12G210880 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.8e-29
Identity = 97/226 (42.92%), Postives = 129/226 (57.08%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAV+A  MN+    QFI P NRD +K  QE  N      T    P I     AV+P    
Sbjct: 1   MAVQAHHMNI--FSQFISP-NRDCVK-FQENMNHGEFEFTGGEVPLITGESFAVEP---- 60

Query: 61  ETTLNNFATMYDWGKAESGLTSN-NFPSTAPTRKRTRSRSLYNEPGGR-----------L 120
                  A   ++ KAESGL+ N   P  +  R+R    S  N P  R           L
Sbjct: 61  ------LAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSL 120

Query: 121 LDEEIINSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEE 180
           ++ E+++    QQQSEIDRF+A   EK+RIE+E R++ ++ ML  A++  + KKLKEK++
Sbjct: 121 INVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDD 180

Query: 181 EIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           EI R+  LNWVLQERVK L VENQ+WRD+A++NEA  N LR NL+Q
Sbjct: 181 EIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQ 212

[Close]

BLAST of Chy12G210880 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.3e-23
Identity = 91/261 (34.87%), Postives = 141/261 (54.02%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTP-GIIWPYTAVKPAVT 60
           MAV+A  + L P   F   +NR+    N  +  T      +  T  G++ P++   P  T
Sbjct: 1   MAVDAHHLFLSPPQLF---SNRELTMNNNTMEPTSGGFCNNNQTGYGVVSPFSV--PNHT 60

Query: 61  PETTLNNFATMYDWGKAESGLTSNNFPSTA-----------PTRKRTR--SRSLYN---- 120
             TT      ++ +G +++  T+  + +             PTRKR+R  SRS Y+    
Sbjct: 61  STTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLL 120

Query: 121 -EPGG----------------RLLDEEI-INSHIQQQQSEIDRFIAIH---REKMRIEME 180
             P                    L ++I I+SH+ QQQ EIDRF+++H    E+++ E+E
Sbjct: 121 QNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIE 180

Query: 181 MRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWVLQERVKRLCVENQVWRDLAESN 223
            ++KR++  ++ AIE+ +VK+L+ KEEE ER+GK+N  L+ERVK L +ENQ+WRDLA++N
Sbjct: 181 EKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTN 240

[Close]

BLAST of Chy12G210880 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.8e-22
Identity = 81/236 (34.32%), Postives = 130/236 (55.08%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFI---KQNQELANTHMTPAT-DTTTPGIIWPYTAVKP 60
           MAVEA  +N       +  +NR+ I   + +  + NT M   T  T  P +    +   P
Sbjct: 1   MAVEAHHLNP------LFSSNREMIHPVEASGVVYNTQMRYGTVPTFNPTVECQTSLFNP 60

Query: 61  A--VTPETTLNNFATMYDWGKAESGLTSN-----NFPSTAPTRKRTRSRSLYNEPGGRLL 120
              ++P   L + +        +S LT N     +F     +RKR+R  S+   P   + 
Sbjct: 61  IYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSREESVVLNPSAYMQ 120

Query: 121 DE-----------EIINSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEK 180
            +           + ++S++QQ   +IDR I+ H E+MR+E+E ++K +   +V A+E+ 
Sbjct: 121 IQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQG 180

Query: 181 VVKKLKEKEEEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           ++K L+ K++EI  +GKLN  L+E+VK LCVENQ+WRD+A+SNEATVN LR+NL+Q
Sbjct: 181 LMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQ 230

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BLAST of Chy12G210880 vs. ExPASy TrEMBL
Match: A0A0A0M3V5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G707120 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.2e-110
Identity = 208/221 (94.12%), Postives = 213/221 (96.38%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNL PSHQFIIPN+RDFIKQNQELANTHMTP TDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGGRLLDEEIINSHIQQ 120
           ETTLNNFAT+YDWGKAESGLTSNNFPSTAPTRKRTRSRS Y+EPGGRLLDEEIINSHIQQ
Sbjct: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120

Query: 121 QQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWVL 180
           QQSE+DRFIAIHREKMRIEMEMRKKRESGMLVRAIEE+VVKKLKEKEEEIERMGKLNWVL
Sbjct: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180

Query: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQASLFVYN 222
           QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ  L   N
Sbjct: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAAN 221

[Close]

BLAST of Chy12G210880 vs. ExPASy TrEMBL
Match: A0A1S3C8U5 (E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo OX=3656 GN=LOC103498284 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.5e-108
Identity = 206/222 (92.79%), Postives = 212/222 (95.50%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNL PSHQFIIPNNRDFIKQNQELA+THMTP TDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNNRDFIKQNQELASTHMTPTTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFA-TMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGGRLLDEEIINSHIQ 120
           E TLNNFA TMYDWGKAESGLTSNNFPSTAPTRKRTRSRS Y+EPGGRLLDEEIINSHIQ
Sbjct: 61  EATLNNFATTMYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ 120

Query: 121 QQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWV 180
           QQQSE+DRFIAIHREKMRIEME+RKKRESGMLVRAIEE+VVKKLKEKEEEIERMGKLNWV
Sbjct: 121 QQQSEMDRFIAIHREKMRIEMEIRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV 180

Query: 181 LQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQASLFVYN 222
           LQERVKRLC+ENQVWRDLAESNEATVNCLRNNLEQ  L   N
Sbjct: 181 LQERVKRLCMENQVWRDLAESNEATVNCLRNNLEQVILMAAN 222

[Close]

BLAST of Chy12G210880 vs. ExPASy TrEMBL
Match: A0A6J1EP88 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434333 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 9.5e-58
Identity = 145/218 (66.51%), Postives = 165/218 (75.69%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN  PS    I NN DF+KQ+QE+ + +        T G +WPY A K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDIYNYKTMPVETAG-LWPYVAGKS--VP 60

Query: 61  ETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEP----GGRLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y EP      RL DEEIINS
Sbjct: 61  E-IVNNF-------PADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKL 180
           H+QQQQSEIDRFIAIHREK+R+ ME+RKKRESGMLVRAIEE++VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE+
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLER 203

[Close]

BLAST of Chy12G210880 vs. ExPASy TrEMBL
Match: A0A6J1KKD3 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495377 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.1e-56
Identity = 144/218 (66.06%), Postives = 164/218 (75.23%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN  PS    I NN DF+KQ+QE+ +          T G +WPY + K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDICNYKTMPAETAG-LWPYISGKS--VP 60

Query: 61  ETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEP----GGRLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y EP      RL DEEIINS
Sbjct: 61  E-IVNNF-------PADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKL 180
           H+QQQQSEIDRFIAIHREK+R+ ME+RKKRESGMLVRAIEE++VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEVRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE+
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLER 203

[Close]

BLAST of Chy12G210880 vs. ExPASy TrEMBL
Match: A0A5D3DZL1 (E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00240 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 3.0e-43
Identity = 93/100 (93.00%), Postives = 95/100 (95.00%), Query Frame = 0


Query: 36  MTPATDTTTPGIIWPYTAVKPAVTPETTLNNFA-TMYDWGKAESGLTSNNFPSTAPTRKR 95
           MTP TDTTTPGIIWPYTAVKPAVTPE TLNNFA TMYDWGKAESGLTSNNFPSTAPTRKR
Sbjct: 1   MTPTTDTTTPGIIWPYTAVKPAVTPEATLNNFATTMYDWGKAESGLTSNNFPSTAPTRKR 60

Query: 96  TRSRSLYNEPGGRLLDEEIINSHIQQQQSEIDRFIAIHRE 135
           TRSRS Y+EPGGRLLDEEIINSHIQQQQSE+DRFIAIH E
Sbjct: 61  TRSRSFYDEPGGRLLDEEIINSHIQQQQSEMDRFIAIHGE 100

[Close]

BLAST of Chy12G210880 vs. NCBI nr
Match: XP_004145480.2 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN66896.1 hypothetical protein Csa_007333 [Cucumis sativus])

HSP 1 Score: 404 bits (1039), Expect = 5.78e-140
Identity = 208/221 (94.12%), Postives = 213/221 (96.38%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNL PSHQFIIPN+RDFIKQNQELANTHMTP TDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNDRDFIKQNQELANTHMTPVTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGGRLLDEEIINSHIQQ 120
           ETTLNNFAT+YDWGKAESGLTSNNFPSTAPTRKRTRSRS Y+EPGGRLLDEEIINSHIQQ
Sbjct: 61  ETTLNNFATVYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQQ 120

Query: 121 QQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWVL 180
           QQSE+DRFIAIHREKMRIEMEMRKKRESGMLVRAIEE+VVKKLKEKEEEIERMGKLNWVL
Sbjct: 121 QQSEMDRFIAIHREKMRIEMEMRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWVL 180

Query: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQASLFVYN 221
           QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ  L   N
Sbjct: 181 QERVKRLCVENQVWRDLAESNEATVNCLRNNLEQVILMAAN 221

[Close]

BLAST of Chy12G210880 vs. NCBI nr
Match: XP_008459064.1 (PREDICTED: E3 ubiquitin-protein ligase BOI-like [Cucumis melo])

HSP 1 Score: 397 bits (1019), Expect = 6.40e-137
Identity = 206/222 (92.79%), Postives = 212/222 (95.50%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEARPMNL PSHQFIIPNNRDFIKQNQELA+THMTP TDTTTPGIIWPYTAVKPAVTP
Sbjct: 1   MAVEARPMNLLPSHQFIIPNNRDFIKQNQELASTHMTPTTDTTTPGIIWPYTAVKPAVTP 60

Query: 61  ETTLNNFAT-MYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGGRLLDEEIINSHIQ 120
           E TLNNFAT MYDWGKAESGLTSNNFPSTAPTRKRTRSRS Y+EPGGRLLDEEIINSHIQ
Sbjct: 61  EATLNNFATTMYDWGKAESGLTSNNFPSTAPTRKRTRSRSFYDEPGGRLLDEEIINSHIQ 120

Query: 121 QQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWV 180
           QQQSE+DRFIAIHREKMRIEME+RKKRESGMLVRAIEE+VVKKLKEKEEEIERMGKLNWV
Sbjct: 121 QQQSEMDRFIAIHREKMRIEMEIRKKRESGMLVRAIEERVVKKLKEKEEEIERMGKLNWV 180

Query: 181 LQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQASLFVYN 221
           LQERVKRLC+ENQVWRDLAESNEATVNCLRNNLEQ  L   N
Sbjct: 181 LQERVKRLCMENQVWRDLAESNEATVNCLRNNLEQVILMAAN 222

[Close]

BLAST of Chy12G210880 vs. NCBI nr
Match: XP_038893892.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida])

HSP 1 Score: 305 bits (780), Expect = 1.28e-100
Identity = 178/234 (76.07%), Postives = 194/234 (82.91%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELA---------NTHMTPATDTTTPGIIWPY 60
           MAVEAR MNL PS QFIIPNNRDFIKQNQ +          NT M PAT+T  PG++ PY
Sbjct: 1   MAVEARRMNLLPS-QFIIPNNRDFIKQNQGIVDICNNHNKTNTQMAPATET--PGLL-PY 60

Query: 61  TAVKPAVTPETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGG----R 120
            A+ P      T+NNFA MYDWGKAESGLTSNNFP+ AP+RKRTR RS+Y+EPGG    R
Sbjct: 61  AALPPE-----TVNNFA-MYDWGKAESGLTSNNFPTVAPSRKRTR-RSVYDEPGGSFPGR 120

Query: 121 LLDEEIINSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKE 180
           LLDEEIINSHIQQQQSEIDRFIAIHREK+R+EME+RKKRESGMLVRAIEE+VVKKLKEKE
Sbjct: 121 LLDEEIINSHIQQQQSEIDRFIAIHREKVRMEMEVRKKRESGMLVRAIEERVVKKLKEKE 180

Query: 181 EEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQASLFVYN 221
           EEIERMGKLNWVLQERVKRLC+ENQVWRDLAESNEATVN LR+NLEQ  L   N
Sbjct: 181 EEIERMGKLNWVLQERVKRLCIENQVWRDLAESNEATVNSLRSNLEQVMLAANN 223

[Close]

BLAST of Chy12G210880 vs. NCBI nr
Match: XP_022927535.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800.1 BOI-related E3 ubiquitin-protein ligase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019425.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 231 bits (588), Expect = 7.60e-72
Identity = 145/218 (66.51%), Postives = 165/218 (75.69%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN  PS    I NN DF+KQ+QE+ + +        T G+ WPY A K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDIYNYKTMPVETAGL-WPYVAGKSV--P 60

Query: 61  ETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGG----RLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y EP      RL DEEIINS
Sbjct: 61  EI-VNNFP-------ADSGLTSNNFPA-APVRKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKL 180
           H+QQQQSEIDRFIAIHREK+R+ ME+RKKRESGMLVRAIEE++VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 214
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE+
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLER 203

[Close]

BLAST of Chy12G210880 vs. NCBI nr
Match: XP_023519053.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229 bits (583), Expect = 4.33e-71
Identity = 145/218 (66.51%), Postives = 164/218 (75.23%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MN  PS    I NN DF+KQ+QE+ +          T G+ WPY A K    P
Sbjct: 1   MAVEARRMNHLPSP--FITNNGDFMKQSQEVLDICNYKTMPVETAGL-WPYVAGKSV--P 60

Query: 61  ETTLNNFATMYDWGKAESGLTSNNFPSTAPTRKRTRSRSLYNEPGG----RLLDEEIINS 120
           E  +NNF        A+SGLTSNNFP+ AP RKR R RS Y EP      RL DEEIINS
Sbjct: 61  EI-VNNFP-------ADSGLTSNNFPA-APARKRQR-RSGYEEPPCPFPCRLSDEEIINS 120

Query: 121 HIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKL 180
           H+QQQQSEIDRFIAIHREK+R+ ME+RKKRESGMLVRAIEE++VKKLKEK+EEIERMGKL
Sbjct: 121 HVQQQQSEIDRFIAIHREKVRMAMEIRKKRESGMLVRAIEERLVKKLKEKDEEIERMGKL 180

Query: 181 NWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 214
           NWVLQERVKRLC+ENQ+WRDLAE+NEATVN LR NLE+
Sbjct: 181 NWVLQERVKRLCMENQLWRDLAETNEATVNSLRTNLER 203

[Close]

BLAST of Chy12G210880 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 132.9 bits (333), Expect = 3.4e-31
Identity = 98/221 (44.34%), Postives = 131/221 (59.28%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAVEAR MNL  S Q+I   NR+ +K     + T+M         G         P    
Sbjct: 1   MAVEARHMNL-FSSQYI--TNRECVK-----SQTNMNNGQQIAGGGF--------PVTIG 60

Query: 61  ETTLNNFATMYDWGKAESGLTS-------NNFPSTAPTRKRTRSRSLYNEPGGRLLDEEI 120
           +  L     +  + K+ES LT+       + F S A    + R R++   P   L+D E+
Sbjct: 61  DRNLQYIDPINSFNKSESELTAISKRQRDSTFDSDALIASQKR-RAIAFSPAS-LIDAEL 120

Query: 121 INSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEEEIERM 180
           + S IQQQ SEIDRF+A   E +RIE+E R++ ++ ML  A++  ++KKLK K+EEI RM
Sbjct: 121 V-SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRM 180

Query: 181 GKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           GKLNWVLQERVK L VENQ+WRDLA++NEAT N LR+NLEQ
Sbjct: 181 GKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQ 202

[Close]

BLAST of Chy12G210880 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 129.0 bits (323), Expect = 4.9e-30
Identity = 97/226 (42.92%), Postives = 129/226 (57.08%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTPGIIWPYTAVKPAVTP 60
           MAV+A  MN+    QFI P NRD +K  QE  N      T    P I     AV+P    
Sbjct: 1   MAVQAHHMNI--FSQFISP-NRDCVK-FQENMNHGEFEFTGGEVPLITGESFAVEP---- 60

Query: 61  ETTLNNFATMYDWGKAESGLTSN-NFPSTAPTRKRTRSRSLYNEPGGR-----------L 120
                  A   ++ KAESGL+ N   P  +  R+R    S  N P  R           L
Sbjct: 61  ------LAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSL 120

Query: 121 LDEEIINSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEE 180
           ++ E+++    QQQSEIDRF+A   EK+RIE+E R++ ++ ML  A++  + KKLKEK++
Sbjct: 121 INVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDD 180

Query: 181 EIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           EI R+  LNWVLQERVK L VENQ+WRD+A++NEA  N LR NL+Q
Sbjct: 181 EIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQ 212

[Close]

BLAST of Chy12G210880 vs. TAIR 10
Match: AT5G45100.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 126.3 bits (316), Expect = 3.2e-29
Identity = 80/166 (48.19%), Postives = 108/166 (65.06%), Query Frame = 0


Query: 56  PAVTPETTLNNFATMYDWGKAESGLTS-------NNFPSTAPTRKRTRSRSLYNEPGGRL 115
           P    +  L     +  + K+ES LT+       + F S A    + R R++   P   L
Sbjct: 13  PVTIGDRNLQYIDPINSFNKSESELTAISKRQRDSTFDSDALIASQKR-RAIAFSPAS-L 72

Query: 116 LDEEIINSHIQQQQSEIDRFIAIHREKMRIEMEMRKKRESGMLVRAIEEKVVKKLKEKEE 175
           +D E++ S IQQQ SEIDRF+A   E +RIE+E R++ ++ ML  A++  ++KKLK K+E
Sbjct: 73  IDAELV-SQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDE 132

Query: 176 EIERMGKLNWVLQERVKRLCVENQVWRDLAESNEATVNCLRNNLEQ 215
           EI RMGKLNWVLQERVK L VENQ+WRDLA++NEAT N LR+NLEQ
Sbjct: 133 EIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQ 175

[Close]

BLAST of Chy12G210880 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 115.2 bits (287), Expect = 7.3e-26
Identity = 91/258 (35.27%), Postives = 141/258 (54.65%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTP-GIIWPYTAVKPAVT 60
           MAV+A  + L P   F   +NR+    N  +  T      +  T  G++ P++   P  T
Sbjct: 1   MAVDAHHLFLSPPQLF---SNRELTMNNNTMEPTSGGFCNNNQTGYGVVSPFSV--PNHT 60

Query: 61  PETTLNNFATMYDWGKAESGLTSNNFPSTA-----------PTRKRTR--SRSLYN---- 120
             TT      ++ +G +++  T+  + +             PTRKR+R  SRS Y+    
Sbjct: 61  STTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLL 120

Query: 121 -EPGG----------------RLLDEEI-INSHIQQQQSEIDRFIAIHREKMRIEMEMRK 180
             P                    L ++I I+SH+ QQQ EIDRF+++H E+++ E+E ++
Sbjct: 121 QNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHMERVKYEIEEKR 180

Query: 181 KRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWVLQERVKRLCVENQVWRDLAESNEAT 223
           KR++  ++ AIE+ +VK+L+ KEEE ER+GK+N  L+ERVK L +ENQ+WRDLA++NEAT
Sbjct: 181 KRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEAT 240

[Close]

BLAST of Chy12G210880 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 109.8 bits (273), Expect = 3.1e-24
Identity = 91/261 (34.87%), Postives = 141/261 (54.02%), Query Frame = 0


Query: 1   MAVEARPMNLPPSHQFIIPNNRDFIKQNQELANTHMTPATDTTTP-GIIWPYTAVKPAVT 60
           MAV+A  + L P   F   +NR+    N  +  T      +  T  G++ P++   P  T
Sbjct: 1   MAVDAHHLFLSPPQLF---SNRELTMNNNTMEPTSGGFCNNNQTGYGVVSPFSV--PNHT 60

Query: 61  PETTLNNFATMYDWGKAESGLTSNNFPSTA-----------PTRKRTR--SRSLYN---- 120
             TT      ++ +G +++  T+  + +             PTRKR+R  SRS Y+    
Sbjct: 61  STTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLL 120

Query: 121 -EPGG----------------RLLDEEI-INSHIQQQQSEIDRFIAIH---REKMRIEME 180
             P                    L ++I I+SH+ QQQ EIDRF+++H    E+++ E+E
Sbjct: 121 QNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIE 180

Query: 181 MRKKRESGMLVRAIEEKVVKKLKEKEEEIERMGKLNWVLQERVKRLCVENQVWRDLAESN 223
            ++KR++  ++ AIE+ +VK+L+ KEEE ER+GK+N  L+ERVK L +ENQ+WRDLA++N
Sbjct: 181 EKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTN 240

The following BLAST results are available for this feature:

BLAST of Chy12G210880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHE4	4.7e-30	44.34	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	6.8e-29	42.92	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
F4IDI6	4.3e-23	34.87	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9LDD1	2.8e-22	34.32	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Chy12G210880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M3V5	2.2e-110	94.12	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G707120 P...	[more]
A0A1S3C8U5	4.5e-108	92.79	E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo OX=3656 GN=LOC103498284 PE=...	[more]
A0A6J1EP88	9.5e-58	66.51	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1KKD3	1.1e-56	66.06	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5D3DZL1	3.0e-43	93.00	E3 ubiquitin-protein ligase BOI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]

BLAST of Chy12G210880 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145480.2	5.78e-140	94.12	BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN66896.1 hypothet...	[more]
XP_008459064.1	6.40e-137	92.79	PREDICTED: E3 ubiquitin-protein ligase BOI-like [Cucumis melo]	[more]
XP_038893892.1	1.28e-100	76.07	BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida]	[more]
XP_022927535.1	7.60e-72	66.51	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita moschata] >KAG6583800....	[more]
XP_023519053.1	4.33e-71	66.51	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy12G210880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45100.1	3.4e-31	44.34	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G19700.1	4.9e-30	42.92	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.2	3.2e-29	48.19	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.2	7.3e-26	35.27	zinc ion binding	[more]
AT1G79110.1	3.1e-24	34.87	zinc ion binding	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..173
None	No IPR available	PANTHER	PTHR42647:SF55	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1	coord: 1..215
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G210880.1	Chy12G210880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity