Chy12G210610 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G210610
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chrH12: 5794320 .. 5795648 (+)
RNA-Seq Expression	Chy12G210610
Synteny	Chy12G210610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACCTTTTCCATTCCAATGCCAAAGGCCTTTCCCCTTTGACCTTAATTTCTATTCTTTGTCTACTACTACAAAATGTGCAAACCACAGGTTTCATTCTCCCTCCCTCTAAATATAATTCATAATTGTACGTTATTCTACAAATAAGTTGAAGAAAAAAAACACATAGCACATGGGAACCGTCTGAGTGGCATCCATATTTTTTTTATCAATACAGGCAACACTCAATAAAAAGTTTGTCATGTAACTGATATTAATGGTTGCACTGAATAATTGTCCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATAACTAAAGTTCAATTCATTTCTAAAAATAATATTGATTTAGTTTATTTTGAATTTTATGTAGGTGCTCAAATCGGTGTCTGCTATGGACAACTCGGCAACGACCTACCATCTCCCGCAGAAGTTATCGACCTTTACAACCAGAACAACATCCATAGAATGCGACTTTACGCTCCAAATCAAGATACTTTCAATGCCCTTCGAGGCTCCAGCATTGAGCTCATGCTTGGCCTTCCCAATGATGAGATTCAATCTATGGCAGCGACCCAAGACAATGCTAATGCATGGATTCAAGACAACATCCTCAACTTTCCCGATGTAAAATTCAAGTACATCGTCGTTGGAAACGAAATAAAAACCAACGAGGAAGCCGCGAAATTTCTCGTGCCCGCCATGCAAAATATCCAAAATGCGATTTCAGCTGTTGGTTTGGAAGGGCAAATCAAAGTCTCCACTGCGTTCCACACTGGAATTCTTTCAGCAGAATCCTTCCCTCCTTCACACGGCTCCTTTGACGCCAACTATCTTCCAATTCTCAACCCGACTATCCGCTTCCTTCTCGACAACAACTCCCCATTGCTCCTCAACTTGTACCCTTACTTTAGTTATGTAGCAACTCCAAACATGGACCTTGATTATGCAATTTTTACTGGGACATCGTTAGTTGAAGATGGGGAGTTTAATTATCAAAACTTGTTTGATGCTATTTTGGATACTGTTTATTCAGCTTCGGAGAAGAATGGTGGAGGATTATTGGAGGTTGTTGTCTCCGAGACCGGTTGGCCGACGGACGGTGGAGAAGCTGCGACGGTTGACAATGCAAGGACTTACAATAACAATCTAATTCAACATGTGAAACAAGGGACTCCAAAGAGACAAGGAAGGGCTATTGAAACTTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACTCCTCCAGAGGTTGAGAGACATTGGGGTTTGTTCTCTCCAAATAAGCAGCCTAAGTATCCAGTCAACTTCAACTAA

mRNA sequence

ATGGCCAACCTTTTCCATTCCAATGCCAAAGGCCTTTCCCCTTTGACCTTAATTTCTATTCTTTGTCTACTACTACAAAATGTGCAAACCACAGGTGCTCAAATCGGTGTCTGCTATGGACAACTCGGCAACGACCTACCATCTCCCGCAGAAGTTATCGACCTTTACAACCAGAACAACATCCATAGAATGCGACTTTACGCTCCAAATCAAGATACTTTCAATGCCCTTCGAGGCTCCAGCATTGAGCTCATGCTTGGCCTTCCCAATGATGAGATTCAATCTATGGCAGCGACCCAAGACAATGCTAATGCATGGATTCAAGACAACATCCTCAACTTTCCCGATGTAAAATTCAAGTACATCGTCGTTGGAAACGAAATAAAAACCAACGAGGAAGCCGCGAAATTTCTCGTGCCCGCCATGCAAAATATCCAAAATGCGATTTCAGCTGTTGGTTTGGAAGGGCAAATCAAAGTCTCCACTGCGTTCCACACTGGAATTCTTTCAGCAGAATCCTTCCCTCCTTCACACGGCTCCTTTGACGCCAACTATCTTCCAATTCTCAACCCGACTATCCGCTTCCTTCTCGACAACAACTCCCCATTGCTCCTCAACTTGTACCCTTACTTTAGTTATGTAGCAACTCCAAACATGGACCTTGATTATGCAATTTTTACTGGGACATCGTTAGTTGAAGATGGGGAGTTTAATTATCAAAACTTGTTTGATGCTATTTTGGATACTGTTTATTCAGCTTCGGAGAAGAATGGTGGAGGATTATTGGAGGTTGTTGTCTCCGAGACCGGTTGGCCGACGGACGGTGGAGAAGCTGCGACGGTTGACAATGCAAGGACTTACAATAACAATCTAATTCAACATGTGAAACAAGGGACTCCAAAGAGACAAGGAAGGGCTATTGAAACTTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACTCCTCCAGAGGTTGAGAGACATTGGGGTTTGTTCTCTCCAAATAAGCAGCCTAAGTATCCAGTCAACTTCAACTAA

Coding sequence (CDS)

ATGGCCAACCTTTTCCATTCCAATGCCAAAGGCCTTTCCCCTTTGACCTTAATTTCTATTCTTTGTCTACTACTACAAAATGTGCAAACCACAGGTGCTCAAATCGGTGTCTGCTATGGACAACTCGGCAACGACCTACCATCTCCCGCAGAAGTTATCGACCTTTACAACCAGAACAACATCCATAGAATGCGACTTTACGCTCCAAATCAAGATACTTTCAATGCCCTTCGAGGCTCCAGCATTGAGCTCATGCTTGGCCTTCCCAATGATGAGATTCAATCTATGGCAGCGACCCAAGACAATGCTAATGCATGGATTCAAGACAACATCCTCAACTTTCCCGATGTAAAATTCAAGTACATCGTCGTTGGAAACGAAATAAAAACCAACGAGGAAGCCGCGAAATTTCTCGTGCCCGCCATGCAAAATATCCAAAATGCGATTTCAGCTGTTGGTTTGGAAGGGCAAATCAAAGTCTCCACTGCGTTCCACACTGGAATTCTTTCAGCAGAATCCTTCCCTCCTTCACACGGCTCCTTTGACGCCAACTATCTTCCAATTCTCAACCCGACTATCCGCTTCCTTCTCGACAACAACTCCCCATTGCTCCTCAACTTGTACCCTTACTTTAGTTATGTAGCAACTCCAAACATGGACCTTGATTATGCAATTTTTACTGGGACATCGTTAGTTGAAGATGGGGAGTTTAATTATCAAAACTTGTTTGATGCTATTTTGGATACTGTTTATTCAGCTTCGGAGAAGAATGGTGGAGGATTATTGGAGGTTGTTGTCTCCGAGACCGGTTGGCCGACGGACGGTGGAGAAGCTGCGACGGTTGACAATGCAAGGACTTACAATAACAATCTAATTCAACATGTGAAACAAGGGACTCCAAAGAGACAAGGAAGGGCTATTGAAACTTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACTCCTCCAGAGGTTGAGAGACATTGGGGTTTGTTCTCTCCAAATAAGCAGCCTAAGTATCCAGTCAACTTCAACTAA

Protein sequence

MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQNLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN*

Homology

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BLAST of Chy12G210610 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 9.6e-112
Identity = 208/341 (61.00%), Postives = 261/341 (76.54%), Query Frame = 0


Query: 15  LTLISILCLLLQNV-------QTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLY 74
           L+LISI+ LL Q V       Q TGA IGVC G +G+DLP  AEV+ LY  NNI RMRLY
Sbjct: 13  LSLISIVLLLGQLVVGSLATKQHTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLY 72

Query: 75  APNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIVVGNE 134
            PN     ALRGS+I+L+LG+PN+ +Q +A +Q NANAW+Q+N+ N+ +VKFKYI VGNE
Sbjct: 73  DPNPAALEALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNE 132

Query: 135 IKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLP 194
           +K ++  A+FLVPAM+NIQ AIS  GL  +IKVSTA  TG+L  E+FPPS GSF + Y  
Sbjct: 133 VKPSDSFAQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVL-GETFPPSIGSFKSEYNA 192

Query: 195 ILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTS-LVEDGEFNYQNLFDA 254
           +L P IRFL+ + SPLL+NLYPYF+Y   T ++ LDYA+FT  S +V+DG F Y+NLFDA
Sbjct: 193 LLYPIIRFLVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDA 252

Query: 255 ILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGR 314
           +LD VY+A EK GGG L+VV+SETGWP+  G A T+DNART+ +NLIQHVK+GTP+R GR
Sbjct: 253 MLDGVYAALEKAGGGSLKVVISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGR 312

Query: 315 AIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
            IETY+FAMFDEN KT  PE+E+HWGLFSP KQPKY ++FN
Sbjct: 313 PIETYIFAMFDENRKT--PELEKHWGLFSPTKQPKYQISFN 350

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BLAST of Chy12G210610 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-108
Identity = 198/347 (57.06%), Postives = 260/347 (74.93%), Query Frame = 0


Query: 5   FHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRM 64
           +HS+ K  S   +  +  LL+    TT AQ GVCYG+LGN+LP+P EV+ LYNQ NI RM
Sbjct: 4   YHSSGKSSSMTAIAFLFILLITYTGTTDAQSGVCYGRLGNNLPTPQEVVALYNQANIRRM 63

Query: 65  RLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFP-DVKFKYIV 124
           R+Y P+ +   ALRGS+IEL+L +PND ++++A++QDNAN W+QDNI N+  +V+F+Y+ 
Sbjct: 64  RIYGPSPEVLEALRGSNIELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYVS 123

Query: 125 VGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDA 184
           VGNE+K     A+FLVPA++NIQ AIS  GL  Q+KVSTA  TG L AESFPPS GSF +
Sbjct: 124 VGNEVKPEHSFAQFLVPALENIQRAISNAGLGNQVKVSTAIDTGAL-AESFPPSKGSFKS 183

Query: 185 NYL-PILNPTIRFLLDNNSPLLLNLYPYFSYVATP-NMDLDYAIFTGTS-LVEDGEFNYQ 244
           +Y    L+  IRFL++NN+PL++N+Y YF+Y A P ++ LDYA+F   S +V+DG   Y+
Sbjct: 184 DYRGAYLDGVIRFLVNNNAPLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYR 243

Query: 245 NLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTP 304
           NLFDA +D VY+A EK GGG L +VVSE+GWP+ GG A ++DNARTYN NL+++VKQGTP
Sbjct: 244 NLFDASVDAVYAALEKAGGGSLNIVVSESGWPSSGGTATSLDNARTYNTNLVRNVKQGTP 303

Query: 305 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSP-NKQPKYPVNFN 347
           KR G  +ETYVFAMFDEN+K   PE E+ WGLFSP  KQPKY +NFN
Sbjct: 304 KRPGAPLETYVFAMFDENQK--QPEFEKFWGLFSPITKQPKYSINFN 347

[Close]

BLAST of Chy12G210610 vs. ExPASy Swiss-Prot
Match: Q9M2M0 (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN=BG1 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.2e-107
Identity = 195/337 (57.86%), Postives = 251/337 (74.48%), Query Frame = 0


Query: 12  LSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQ 71
           L+ LTL  +L L   N   TG Q+GVCYG+ GN+LPSPAE I L+ Q NI R+RLY+P+ 
Sbjct: 6   LASLTL--LLGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDH 65

Query: 72  DTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPD-VKFKYIVVGNEIKT 131
           D   ALRGS+IE+ LGLPN  +QS+A++Q  ANAW+Q  ++N+ + V+F+YI VGNE+K 
Sbjct: 66  DVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKI 125

Query: 132 NEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILN 191
           ++  A+FLVPAM+NI  A+ A GL G+IKVST+   G+L  ES+PPS GSF  + + ++ 
Sbjct: 126 SDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVL-RESYPPSKGSFRGDVMVVME 185

Query: 192 PTIRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTS-LVEDGEFNYQNLFDAILD 251
           P IRFL+  NSPLLLNLY YFSY      + LDYA+FT  S +V D   +YQNLFDA+LD
Sbjct: 186 PIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLD 245

Query: 252 TVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIE 311
            +YSA EK+GG  LE+VV+ETGWPT GG    ++NAR YNNNLI+HVK GTPKR G+ IE
Sbjct: 246 AMYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIE 305

Query: 312 TYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNF 346
           TY+FA++DEN+K TPP VE+ WGLF PNKQPKY +NF
Sbjct: 306 TYLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Chy12G210610 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.6e-98
Identity = 183/337 (54.30%), Postives = 245/337 (72.70%), Query Frame = 0


Query: 15  LTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDTF 74
           +TL+ +  L L  +  TGAQ GVCYG+ GN LPSPA+V+ L N+NNI RMR+Y P+Q T 
Sbjct: 6   VTLLLLSVLTLATLDFTGAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTL 65

Query: 75  NALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIVVGNEIKTNEEA 134
            ALRGS+IELMLG+PN +++++AA+Q NA+ W+Q+N+ N+ +VKF+YI VGNE+    E 
Sbjct: 66  EALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNEN 125

Query: 135 AKF---LVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNP 194
           +K+   L+ AM+NIQ AIS  GL  QIKVSTA  TG L+ ++ PPS+G F  +    + P
Sbjct: 126 SKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETG-LTTDTSPPSNGRFKDDVRQFIEP 185

Query: 195 TIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTS-LVEDGEFNYQNLFDAILDTV 254
            I FL+ N +PLL+NLYPYF+     ++ L+YA+FT +  +V D    Y+NLFDAILD  
Sbjct: 186 IINFLVTNRAPLLVNLYPYFAIANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDAT 245

Query: 255 YSASEKNGGGLLEVVVSETGWPTDG-GEAATVDNARTYNNNLIQHVKQGTPKRQGRAIET 314
           YSA EK  G  LE+VVSE+GWP+ G G+  ++DNARTYNNNLI HVK G+PKR    IET
Sbjct: 246 YSALEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIET 305

Query: 315 YVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           YVFA+FDE++K   PE+E+H+GLFS N QPKY ++FN
Sbjct: 306 YVFALFDEDQK--DPEIEKHFGLFSANMQPKYQISFN 339

[Close]

BLAST of Chy12G210610 vs. ExPASy Swiss-Prot
Match: F4J270 (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN=BG3 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.3e-97
Identity = 185/335 (55.22%), Postives = 239/335 (71.34%), Query Frame = 0


Query: 14  PLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDT 73
           PL L+ +  +L     T   QIGVCYG+ GN+L   +EV+ LY Q NI RMRLY PNQ+T
Sbjct: 14  PLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQET 73

Query: 74  FNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIVVGNEIKTNEE 133
            NALRGS+IEL+L +PN ++Q +A++Q  A+ W+++N+ N+ +V F+YI VGNE++ +++
Sbjct: 74  LNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQ 133

Query: 134 AAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTI 193
           AA F++PAMQNI+ A+S++G    IKVSTA  T  +S   FPPS G+F   +   + P I
Sbjct: 134 AASFVLPAMQNIERAVSSLG----IKVSTAIDTRGIS--GFPPSSGTFTPEFRSFIAPVI 193

Query: 194 RFLLDNNSPLLLNLYPYFSYVAT-PNMDLDYAIFTGTS-LVEDGEFNYQNLFDAILDTVY 253
            FL    SPLL+N YPYFSY     ++ LDY +FT  S +V DG+  Y+NLF AILDTVY
Sbjct: 194 SFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVY 253

Query: 254 SASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYV 313
           ++ EK GGG LE+VVSE+GWPT GG A  VDNARTY NNLIQ VK G+P+R GRA ETY+
Sbjct: 254 ASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYI 313

Query: 314 FAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           FAMFDEN K   PE E+ WGLF PN QPKY VNFN
Sbjct: 314 FAMFDENSK-QGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Chy12G210610 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.5e-187
Identity = 328/340 (96.47%), Postives = 334/340 (98.24%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLFHSNAKGLS LTLISILCLLLQN+QTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQDTFNALRGSSIELMLGLPND+IQSMAATQDNANAWIQDNILNF DV FK
Sbjct: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAA+FLVPAMQNIQNAISAVGL+GQIKVSTAFHTGILSAESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQ 240
           FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNM+LDYAIFTGTSLVEDGEFNYQ
Sbjct: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240

Query: 241 NLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLFDAILDTVYSA EKNGGG LEVVVSETGWPT+GGEAATVDNARTYNNNLIQHVKQGTP
Sbjct: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK 341
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK 340

[Close]

BLAST of Chy12G210610 vs. ExPASy TrEMBL
Match: A0A1S3C9Z3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.5e-179
Identity = 316/346 (91.33%), Postives = 329/346 (95.09%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLF S+ KGLS LTLISILCLLL+N+QTT AQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFPSSDKGLSTLTLISILCLLLKNLQTTVAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQ TFNALRGSSIELMLGLPNDEIQSMAATQDNANAW+QDNILNFPDV FK
Sbjct: 61  IQRMRLYAPNQATFNALRGSSIELMLGLPNDEIQSMAATQDNANAWVQDNILNFPDVNFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAA+FLVPAMQNIQNAISAVGL G+IKVSTAFHTGILS ESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLGGKIKVSTAFHTGILSVESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQ 240
           FDA+YLPILNPTIRFLLDNNSPLL+NLYPYFSYV TP+M+LDYAIFTGTSLV DGEFNYQ
Sbjct: 181 FDASYLPILNPTIRFLLDNNSPLLVNLYPYFSYVGTPSMELDYAIFTGTSLVVDGEFNYQ 240

Query: 241 NLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLF AILDTVYSA EKNGGG LE+VVSETGWPTDGGEAATVDNARTY NNLIQ VKQGTP
Sbjct: 241 NLFHAILDTVYSALEKNGGGSLEIVVSETGWPTDGGEAATVDNARTYTNNLIQRVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK+ V+FN
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKFSVSFN 346

[Close]

BLAST of Chy12G210610 vs. ExPASy TrEMBL
Match: A0A5D3BSK7 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1734G00070 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.1e-147
Identity = 261/283 (92.23%), Postives = 271/283 (95.76%), Query Frame = 0


Query: 64  MRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIV 123
           MRLYAPNQ TFNALRGSSIELMLGLPNDEIQSMAATQDNANAW+QDNILNFPDV FKYIV
Sbjct: 1   MRLYAPNQATFNALRGSSIELMLGLPNDEIQSMAATQDNANAWVQDNILNFPDVNFKYIV 60

Query: 124 VGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDA 183
           VGNEIKTNEEAA+FLVPAMQNIQNAISAVGL G+IKVSTAFHTGILS ESFPPSHGSFDA
Sbjct: 61  VGNEIKTNEEAARFLVPAMQNIQNAISAVGLGGKIKVSTAFHTGILSVESFPPSHGSFDA 120

Query: 184 NYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQNLF 243
           +YLPILNPTIRFLLDNNSPLL+NLYPYFSYV TP+M+LDYAIFTGTSLV DGEFNYQNLF
Sbjct: 121 SYLPILNPTIRFLLDNNSPLLVNLYPYFSYVGTPSMELDYAIFTGTSLVVDGEFNYQNLF 180

Query: 244 DAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQ 303
            AILDTVYSA EKNGGG LE+VVSETGWPTDGGEAATVDNARTY NNLIQ VKQGTPKRQ
Sbjct: 181 HAILDTVYSALEKNGGGSLEIVVSETGWPTDGGEAATVDNARTYTNNLIQRVKQGTPKRQ 240

Query: 304 GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK+ V+FN
Sbjct: 241 GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKFSVSFN 283

[Close]

BLAST of Chy12G210610 vs. ExPASy TrEMBL
Match: A0A6J1CBL7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.9e-144
Identity = 258/347 (74.35%), Postives = 298/347 (85.88%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MAN F++NAKG S      +  LL+ N+  T AQIGVCYGQLG+DLPSP EVIDLYNQNN
Sbjct: 1   MAN-FYANAKGFSIFFSFLLWLLLIANLHLTNAQIGVCYGQLGDDLPSPVEVIDLYNQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQ + NALRGS+IELMLG+PN E+++MA TQ+NAN+W QDN+L+F DVKFK
Sbjct: 61  IERMRLYAPNQPSLNALRGSNIELMLGIPNHELEAMATTQENANSWFQDNVLSFGDVKFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIK ++ AA+FLVPAMQNIQNAISA GL G+IKVSTA  TG+L AES+PPS GS
Sbjct: 121 YIVVGNEIKPSDGAARFLVPAMQNIQNAISAAGLRGKIKVSTAIDTGVL-AESYPPSKGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTSLVEDGEFNY 240
           F ++YLPIL+PTIRFL+DNNSPLLLNLY YFS+++ T ++ LDYA+FTG S+V DG+  Y
Sbjct: 181 FRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSHISNTKDISLDYALFTGMSMVSDGQLVY 240

Query: 241 QNLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGT 300
           QNLFDAILD VYSA EK+ GG LE+VVSE+GWPTDGGEAA VDNARTYNNNLIQHVK GT
Sbjct: 241 QNLFDAILDAVYSALEKSAGGGLEIVVSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGT 300

Query: 301 PKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           PKR G  IETYVFAMFDEN+KTTPPEVE+HWGLFSPNKQPKY VNF+
Sbjct: 301 PKRPGMPIETYVFAMFDENQKTTPPEVEKHWGLFSPNKQPKYLVNFH 345

[Close]

BLAST of Chy12G210610 vs. ExPASy TrEMBL
Match: A0A6J1HCY3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.5e-141
Identity = 255/345 (73.91%), Postives = 295/345 (85.51%), Query Frame = 0


Query: 4   LFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHR 63
           +F SNA GLS    +S+L LL  ++  T AQIGVCYGQLGNDLPSP EVI+LY QNNI R
Sbjct: 3   IFFSNANGLSTF-FLSLLWLLFASMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQR 62

Query: 64  MRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIV 123
           MRLYA N  TF+ALRGS+IELMLG+PN+E++ +A  Q+ A++W+ DN+++F DVKFKYIV
Sbjct: 63  MRLYALNHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIV 122

Query: 124 VGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDA 183
           VGNEIKT ++AA FLVPAMQNIQNAISAVGLEGQIKVSTAF TGIL AES+PPS GSF  
Sbjct: 123 VGNEIKTYDQAASFLVPAMQNIQNAISAVGLEGQIKVSTAFDTGIL-AESYPPSKGSFKT 182

Query: 184 NYLPILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTS-LVEDGEFNYQN 243
           +YLPILNPTIRFLLDNN+PLL+N+YPYFSYVA T ++ +DYA+F G S  VEDG+F Y N
Sbjct: 183 DYLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGASPSVEDGQFVYHN 242

Query: 244 LFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPK 303
           LFDAILD VYSA EK GG  LE+VVSETGWPT+GGEAATV+NA TYNNNLIQHVK+GTPK
Sbjct: 243 LFDAILDAVYSALEKAGGDGLEIVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPK 302

Query: 304 RQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           R G+ +ETYVFAMFDENEKTTPPEVE+HWGLFSP+K PKY VNFN
Sbjct: 303 RSGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

[Close]

BLAST of Chy12G210610 vs. NCBI nr
Match: XP_011659353.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus] >KAE8653333.1 hypothetical protein Csa_023295 [Cucumis sativus])

HSP 1 Score: 672 bits (1735), Expect = 1.77e-243
Identity = 334/346 (96.53%), Postives = 340/346 (98.27%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLFHSNAKGLS LTLISILCLLLQN+QTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQDTFNALRGSSIELMLGLPND+IQSMAATQDNANAWIQDNILNF DV FK
Sbjct: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAA+FLVPAMQNIQNAISAVGL+GQIKVSTAFHTGILSAESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQ 240
           FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNM+LDYAIFTGTSLVEDGEFNYQ
Sbjct: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240

Query: 241 NLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLFDAILDTVYSA EKNGGG LEVVVSETGWPT+GGEAATVDNARTYNNNLIQHVKQGTP
Sbjct: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346

[Close]

BLAST of Chy12G210610 vs. NCBI nr
Match: XP_008459107.1 (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 633 bits (1632), Expect = 8.85e-228
Identity = 316/346 (91.33%), Postives = 329/346 (95.09%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLF S+ KGLS LTLISILCLLL+N+QTT AQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFPSSDKGLSTLTLISILCLLLKNLQTTVAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQ TFNALRGSSIELMLGLPNDEIQSMAATQDNANAW+QDNILNFPDV FK
Sbjct: 61  IQRMRLYAPNQATFNALRGSSIELMLGLPNDEIQSMAATQDNANAWVQDNILNFPDVNFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAA+FLVPAMQNIQNAISAVGL G+IKVSTAFHTGILS ESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLGGKIKVSTAFHTGILSVESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQ 240
           FDA+YLPILNPTIRFLLDNNSPLL+NLYPYFSYV TP+M+LDYAIFTGTSLV DGEFNYQ
Sbjct: 181 FDASYLPILNPTIRFLLDNNSPLLVNLYPYFSYVGTPSMELDYAIFTGTSLVVDGEFNYQ 240

Query: 241 NLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLF AILDTVYSA EKNGGG LE+VVSETGWPTDGGEAATVDNARTY NNLIQ VKQGTP
Sbjct: 241 NLFHAILDTVYSALEKNGGGSLEIVVSETGWPTDGGEAATVDNARTYTNNLIQRVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK+ V+FN
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKFSVSFN 346

[Close]

BLAST of Chy12G210610 vs. NCBI nr
Match: XP_038895085.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 596 bits (1537), Expect = 2.54e-213
Identity = 295/346 (85.26%), Postives = 317/346 (91.62%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLFHSNAK LS LT +S+L L L N+Q  GA+IGVCYGQLGNDLPSP EVI+LY QNN
Sbjct: 1   MANLFHSNAKALSTLTFLSLLSLQLANLQIAGAEIGVCYGQLGNDLPSPQEVIELYKQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQ TF+ALRGS+IELMLG+PND+I+SMAATQDNANAW+Q+NILNFPDVKFK
Sbjct: 61  IQRMRLYAPNQPTFDALRGSNIELMLGIPNDQIRSMAATQDNANAWVQENILNFPDVKFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAA FLVPAMQNIQNAISAVGL+GQIKVSTAF TGILS +SFPPS GS
Sbjct: 121 YIVVGNEIKTNEEAATFLVPAMQNIQNAISAVGLQGQIKVSTAFDTGILS-QSFPPSQGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQ 240
           FD NYLPILNPT RFLLDN+SPLL+NLYPYFSYV TP M LDYAIFTGTSL+ DGE NYQ
Sbjct: 181 FDTNYLPILNPTFRFLLDNSSPLLVNLYPYFSYVTTPRMGLDYAIFTGTSLIGDGELNYQ 240

Query: 241 NLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLFDAILD VYSA EK+GGG LE+VVSETGWPTD G+AATVDNARTYNNNLIQHV QGTP
Sbjct: 241 NLFDAILDAVYSALEKSGGGSLEIVVSETGWPTDHGDAATVDNARTYNNNLIQHVNQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346
           KR+GRAIETY+FAMFDENEKTTPPEVERHWGLFSPNKQPKY VNFN
Sbjct: 301 KRRGRAIETYLFAMFDENEKTTPPEVERHWGLFSPNKQPKYSVNFN 345

[Close]

BLAST of Chy12G210610 vs. NCBI nr
Match: KAA0043333.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa] >TYK02663.1 glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 530 bits (1365), Expect = 4.10e-188
Identity = 261/283 (92.23%), Postives = 271/283 (95.76%), Query Frame = 0


Query: 64  MRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIV 123
           MRLYAPNQ TFNALRGSSIELMLGLPNDEIQSMAATQDNANAW+QDNILNFPDV FKYIV
Sbjct: 1   MRLYAPNQATFNALRGSSIELMLGLPNDEIQSMAATQDNANAWVQDNILNFPDVNFKYIV 60

Query: 124 VGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDA 183
           VGNEIKTNEEAA+FLVPAMQNIQNAISAVGL G+IKVSTAFHTGILS ESFPPSHGSFDA
Sbjct: 61  VGNEIKTNEEAARFLVPAMQNIQNAISAVGLGGKIKVSTAFHTGILSVESFPPSHGSFDA 120

Query: 184 NYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMDLDYAIFTGTSLVEDGEFNYQNLF 243
           +YLPILNPTIRFLLDNNSPLL+NLYPYFSYV TP+M+LDYAIFTGTSLV DGEFNYQNLF
Sbjct: 121 SYLPILNPTIRFLLDNNSPLLVNLYPYFSYVGTPSMELDYAIFTGTSLVVDGEFNYQNLF 180

Query: 244 DAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQ 303
            AILDTVYSA EKNGGG LE+VVSETGWPTDGGEAATVDNARTY NNLIQ VKQGTPKRQ
Sbjct: 181 HAILDTVYSALEKNGGGSLEIVVSETGWPTDGGEAATVDNARTYTNNLIQRVKQGTPKRQ 240

Query: 304 GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346
           GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK+ V+FN
Sbjct: 241 GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKFSVSFN 283

[Close]

BLAST of Chy12G210610 vs. NCBI nr
Match: XP_022139185.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 514 bits (1325), Expect = 4.86e-181
Identity = 258/347 (74.35%), Postives = 298/347 (85.88%), Query Frame = 0


Query: 1   MANLFHSNAKGLSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MAN F++NAKG S      +  LL+ N+  T AQIGVCYGQLG+DLPSP EVIDLYNQNN
Sbjct: 1   MAN-FYANAKGFSIFFSFLLWLLLIANLHLTNAQIGVCYGQLGDDLPSPVEVIDLYNQNN 60

Query: 61  IHRMRLYAPNQDTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFK 120
           I RMRLYAPNQ + NALRGS+IELMLG+PN E+++MA TQ+NAN+W QDN+L+F DVKFK
Sbjct: 61  IERMRLYAPNQPSLNALRGSNIELMLGIPNHELEAMATTQENANSWFQDNVLSFGDVKFK 120

Query: 121 YIVVGNEIKTNEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIK ++ AA+FLVPAMQNIQNAISA GL G+IKVSTA  TG+L AES+PPS GS
Sbjct: 121 YIVVGNEIKPSDGAARFLVPAMQNIQNAISAAGLRGKIKVSTAIDTGVL-AESYPPSKGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTSLVEDGEFNY 240
           F ++YLPIL+PTIRFL+DNNSPLLLNLY YFS+++ T ++ LDYA+FTG S+V DG+  Y
Sbjct: 181 FRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSHISNTKDISLDYALFTGMSMVSDGQLVY 240

Query: 241 QNLFDAILDTVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGT 300
           QNLFDAILD VYSA EK+ GG LE+VVSE+GWPTDGGEAA VDNARTYNNNLIQHVK GT
Sbjct: 241 QNLFDAILDAVYSALEKSAGGGLEIVVSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGT 300

Query: 301 PKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346
           PKR G  IETYVFAMFDEN+KTTPPEVE+HWGLFSPNKQPKY VNF+
Sbjct: 301 PKRPGMPIETYVFAMFDENQKTTPPEVEKHWGLFSPNKQPKYLVNFH 345

[Close]

BLAST of Chy12G210610 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 389.8 bits (1000), Expect = 2.3e-108
Identity = 195/337 (57.86%), Postives = 251/337 (74.48%), Query Frame = 0


Query: 12  LSPLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQ 71
           L+ LTL  +L L   N   TG Q+GVCYG+ GN+LPSPAE I L+ Q NI R+RLY+P+ 
Sbjct: 6   LASLTL--LLGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDH 65

Query: 72  DTFNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPD-VKFKYIVVGNEIKT 131
           D   ALRGS+IE+ LGLPN  +QS+A++Q  ANAW+Q  ++N+ + V+F+YI VGNE+K 
Sbjct: 66  DVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKI 125

Query: 132 NEEAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILN 191
           ++  A+FLVPAM+NI  A+ A GL G+IKVST+   G+L  ES+PPS GSF  + + ++ 
Sbjct: 126 SDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVL-RESYPPSKGSFRGDVMVVME 185

Query: 192 PTIRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTS-LVEDGEFNYQNLFDAILD 251
           P IRFL+  NSPLLLNLY YFSY      + LDYA+FT  S +V D   +YQNLFDA+LD
Sbjct: 186 PIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLD 245

Query: 252 TVYSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIE 311
            +YSA EK+GG  LE+VV+ETGWPT GG    ++NAR YNNNLI+HVK GTPKR G+ IE
Sbjct: 246 AMYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIE 305

Query: 312 TYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNF 346
           TY+FA++DEN+K TPP VE+ WGLF PNKQPKY +NF
Sbjct: 306 TYLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Chy12G210610 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 357.1 bits (915), Expect = 1.6e-98
Identity = 185/335 (55.22%), Postives = 239/335 (71.34%), Query Frame = 0


Query: 14  PLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDT 73
           PL L+ +  +L     T   QIGVCYG+ GN+L   +EV+ LY Q NI RMRLY PNQ+T
Sbjct: 14  PLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQET 73

Query: 74  FNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPDVKFKYIVVGNEIKTNEE 133
            NALRGS+IEL+L +PN ++Q +A++Q  A+ W+++N+ N+ +V F+YI VGNE++ +++
Sbjct: 74  LNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQ 133

Query: 134 AAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTI 193
           AA F++PAMQNI+ A+S++G    IKVSTA  T  +S   FPPS G+F   +   + P I
Sbjct: 134 AASFVLPAMQNIERAVSSLG----IKVSTAIDTRGIS--GFPPSSGTFTPEFRSFIAPVI 193

Query: 194 RFLLDNNSPLLLNLYPYFSYVAT-PNMDLDYAIFTGTS-LVEDGEFNYQNLFDAILDTVY 253
            FL    SPLL+N YPYFSY     ++ LDY +FT  S +V DG+  Y+NLF AILDTVY
Sbjct: 194 SFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVY 253

Query: 254 SASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYV 313
           ++ EK GGG LE+VVSE+GWPT GG A  VDNARTY NNLIQ VK G+P+R GRA ETY+
Sbjct: 254 ASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYI 313

Query: 314 FAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           FAMFDEN K   PE E+ WGLF PN QPKY VNFN
Sbjct: 314 FAMFDENSK-QGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Chy12G210610 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 349.0 bits (894), Expect = 4.5e-96
Identity = 180/336 (53.57%), Postives = 239/336 (71.13%), Query Frame = 0


Query: 14  PLTLISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDT 73
           P+ +I +  ++      T  QIGVCYG LG+ LPSP++V+ LY Q NI RMRLY P+   
Sbjct: 11  PMLMILLSLVIASFFNHTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGA 70

Query: 74  FNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILNFPD-VKFKYIVVGNEIKTNE 133
             ALRGS IEL+L +P+ +++ +A++Q  A+ W+Q+N+ ++ D V+F+YI VGNE+K + 
Sbjct: 71  LAALRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS- 130

Query: 134 EAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPT 193
               FL+ AMQNI+NA+S  GLE  +KVSTA  T   + ++ PPS G F   Y   L P 
Sbjct: 131 -VGGFLLQAMQNIENAVSGAGLE--VKVSTAIATD-TTTDTSPPSQGRFRDEYKSFLEPV 190

Query: 194 IRFLLDNNSPLLLNLYPYFSYVA-TPNMDLDYAIFTGTSLVE-DGEFNYQNLFDAILDTV 253
           I FL    SPLL+NLYPYFSY+  T N+ LDYA+FT  S V+ D  ++YQNLFDA LD+V
Sbjct: 191 IGFLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSV 250

Query: 254 YSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETY 313
           Y+A EK+GGG LE+VVSETGWPT+G    +V+NA+TY NNLIQHVK G+P+R G+AIETY
Sbjct: 251 YAALEKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETY 310

Query: 314 VFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           +FAMFDEN+K   P  E+ WGLF P++Q KY VNFN
Sbjct: 311 IFAMFDENKK--EPTYEKFWGLFHPDRQSKYEVNFN 339

[Close]

BLAST of Chy12G210610 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-89
Identity = 168/338 (49.70%), Postives = 236/338 (69.82%), Query Frame = 0


Query: 16  TLISILCLLLQNV--QTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDT 75
           TL  ++ L +  +   T+G  +GVCYG +GN+LPS ++ I L+ QNNI R+RLY PNQ  
Sbjct: 3   TLFLLIALFITTILNPTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAA 62

Query: 76  FNALRGSSIELMLGLPNDEIQSMAATQDNANAWIQDNILN-FPDVKFKYIVVGNEIKTNE 135
            NALR + IE+++G+PN +++S+     +A +W+Q+N+LN +P V FKYI VGNE+  + 
Sbjct: 63  LNALRNTGIEVIIGVPNTDLRSL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPS- 122

Query: 136 EAAKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPT 195
                ++PAM+N+ +A+    L+ +IKVSTA    ++   SFPPS G F  +    ++P 
Sbjct: 123 NGGDVVLPAMRNVYDALRGANLQDRIKVSTAIDMTLI-GNSFPPSSGEFRGDVRWYIDPV 182

Query: 196 IRFLLDNNSPLLLNLYPYFSYVATP-NMDLDYAIFTGTSLVE-DGEFNYQNLFDAILDTV 255
           I FL   NS LL N+YPYFSYV  P ++ L YA+FT  S+V  DG   YQNLFDA+LD V
Sbjct: 183 IGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVV 242

Query: 256 YSASEKNGGGLLEVVVSETGWPTDGGEAATVDNARTYNNNLIQHVKQ--GTPKRQGRAIE 315
           YSA E++GGG L VVVSE+GWP++GG AA+ DNAR +  NL   V++  GTPKR GR +E
Sbjct: 243 YSAVERSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVE 302

Query: 316 TYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           TY+FAMFDEN+K+  PE+E+++GLF PNKQPK+P+ F+
Sbjct: 303 TYLFAMFDENQKS--PEIEKNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of Chy12G210610 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.1e-70
Identity = 144/340 (42.35%), Postives = 216/340 (63.53%), Query Frame = 0


Query: 18  ISILCLLLQNVQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIHRMRLYAPNQDTFNAL 77
           ISIL LLL      G ++GVCYG+  +DLP+P++V+ L  Q+NI  +R+Y  N     A 
Sbjct: 11  ISILLLLLDCCY--GGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAF 70

Query: 78  RGSSIELMLGLPNDEIQSMAATQDNANAWIQDNIL-NFPDVKFKYIVVGNEIKTNE--EA 137
             +SIELM+G+PN ++ + + +Q N + W+++++L  +P  K  YI VG E   +    A
Sbjct: 71  GNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINA 130

Query: 138 AKFLVPAMQNIQNAISAVGLEGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIR 197
           + F+VPAMQN+  A+  VGL  +IKVST    GILS  SFPPS G+F+++Y   L P + 
Sbjct: 131 SSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILS-RSFPPSAGAFNSSYAYFLRPMLE 190

Query: 198 FLLDNNSPLLLNLYPYFSYVATP-NMDLDYAIFTGTSLVEDGEFN--YQNLFDAILDTVY 257
           FL +N SP +++LYPY++Y  +P N+ LDY +F  +S V D      Y+N+FDA +D +Y
Sbjct: 191 FLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALY 250

Query: 258 SASEKNGGGLLEVVVSETGWPTDGG----EAATVDNARTYNNNLIQHV--KQGTPKRQGR 317
            A        ++++V+ETGWPT G      AA+ DNA TYN+N+I+HV   QGTP + G 
Sbjct: 251 YALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGE 310

Query: 318 AIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNF 346
           A+  Y+F++F+EN K    + ER+WGLF P++   Y ++F
Sbjct: 311 AMNVYIFSLFNENRK-AGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:

BLAST of Chy12G210610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52408	9.6e-112	61.00	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]
Q03773	1.3e-108	57.06	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
Q9M2M0	3.2e-107	57.86	Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
P36401	1.6e-98	54.30	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]
F4J270	2.3e-97	55.22	Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Chy12G210610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYQ8	5.5e-187	96.47	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3...	[more]
A0A1S3C9Z3	2.5e-179	91.33	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV...	[more]
A0A5D3BSK7	1.1e-147	92.23	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1CBL7	9.9e-144	74.35	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 ...	[more]
A0A6J1HCY3	3.5e-141	73.91	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 P...	[more]

BLAST of Chy12G210610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659353.1	1.77e-243	96.53	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus] >KAE86533...	[more]
XP_008459107.1	8.85e-228	91.33	PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo]	[more]
XP_038895085.1	2.54e-213	85.26	glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida]	[more]
KAA0043333.1	4.10e-188	92.23	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa] ...	[more]
XP_022139185.1	4.86e-181	74.35	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]

BLAST of Chy12G210610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57270.1	2.3e-108	57.86	beta-1,3-glucanase 1	[more]
AT3G57240.1	1.6e-98	55.22	beta-1,3-glucanase 3	[more]
AT3G57260.1	4.5e-96	53.57	beta-1,3-glucanase 2	[more]
AT4G16260.1	1.1e-89	49.70	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	1.1e-70	42.35	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 35..345 e-value: 4.7E-115 score: 384.5
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 262..275
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 33..346 e-value: 2.4E-130 score: 436.2
None	No IPR available	PANTHER	PTHR32227:SF138	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED	coord: 28..343
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 28..343
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 35..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G210610.1	Chy12G210610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds