Chy12G210580 (gene) Cucumber (hystrix) v1

Overview
NameChy12G210580
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptiongamma-soluble NSF attachment protein
LocationchrH12: 5700389 .. 5705616 (+)
RNA-Seq ExpressionChy12G210580
SyntenyChy12G210580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGGTGCCGAGCTTCTGAGGTTCTTATGGGATTTGGGTTTTGTTTTTTTTTTCCCTTTTAGTTTTGATTATGGATTCTGTAAGGGCTAGTTTGAAGTACCTTTCAGAAATGGGAAGGAAAATGATAAACGGAATAAATGAGCCTATGACAATGTGGGCTTTAAGCCATGCTTAATGTTGATGTGTAATTCTAGTGTTAGTTTCGAGGTGGATCGGATGGGTAGTTCATTTTTCAGGATCCATCTTTGAACTTATCCATTAAGTTTTCTTTGAATTATATTAATTTTCTTTATTTCCTGTAAAATCTACAGAACAAAACTCAGTATGACAAGATGGAGTGCTGATTGGAAAAGTGCTACTGTCCTCTATGAGCAGGCCGGTATGTTTATTTTCTAATCATGCAGTTACTTAAGCTCTCTAATGTGTTGCAAGATCATGAAACTTGCAGATTTTTGTATTACTTGTTATAATCTGTTCTCCCCAGATGATTTTAAATAAGAATCTGTCTCTTTGCTTTAGCAAATGCATACCGGGTCCAAAATAACCATGAGAAAGCAAAGATTGCTTTTGAAAAAGCTTCAAAAGGGCAAGAGATGCTCTCCTCGTATCCTTGTTTCTAGAATTGGTTAATGGCTCTTTGGCCTGCATTCATTTATTTCTTGACCTTTGTTTTCTCCCTTTAAGTCCTTACTACTAATCAGACCCTGGGATGCCGCTAAGCATATGGAATCTGCTGGTGCTTTCGCAAAGGAACTTGGTAACTTGACTGAAGTTGCTGACTTTTACAGAAGAGCATCCGAGTTGTATGTTTTGTGTGGAAGATCTCAACCTGCATCTGATGCTTTATCAAAGGGTGCTCAGTAAGTTAATGTTTTATGCAACTTCACATAATTTTTGTCATTCAACCAAATTGCTTGAAACTAGATTTCTTCTTTTGTATCCTTTTATTCTTAACTAAATTGTTTCTTAACCACATAAAGGCTCTAAGTTAGCTTCGTGCAGTTGACAACATTATCAAAAATAAATTATATATAATTTATACCTATAGTTTTTTTCAGTAGTTCTCTAGTAGAATGTTGGTTGTTTACGTTTCTTTGCCATTGCTGTTTCTCTAGCAGTAGGTTTCTCTCACCCAATTGAACAGAAGTTTCTTTCCTGTCCAAATTTTTTTAGAAGAACAATATCTTTGTTTAACCTACTTTACCTCTATATTGGCTCAATTAGTTTATATTCACCACAACTCCTTATCATTTATGCCTACCGTCCTTCTTATACACAATTGCATTTGCCTTTTATTTGTTCATTCATGCTTGAGCAGCCACAGGTTAAAAACAAGAATAATAAAAAAGCTCCTCATTGAACTATTGCATCCTCTCTTCACTGGGATGGATGAACCATAACTGTAACTTAAGGGAATATGTCATTGCTTTTATATTTTACAAACATACAAGTTGATATGAGTACATCTTGCCATAAATTCCCTTGAGCATTTTAATTATATATCTTCTTACAGTGCTTTGGAAGATAGCAAGCCAGATGAAGCTATCACATTATACACTGAGGCCTGTACACTTCTGGAAGATGATGGCAGGGAACAAATGGCCTTTGATTTATATCGAGATGCCACAAGTGTTTTTGTAAAATTAGAGAAGTAAGTTTCAAGTAATTTTGAAACTGCCATAATCAAAATCTTAAAGTATTAACTTATTAGTTAGTTCACTATCTTGTTATCTGCTATTTCATAATGAAATTTTTATTACTCATGTGCTGTCGAAACTACAATAGACGTTGTAGTAAAGGTGACAGCTAAACTTGCCCCTTGGACCCTAGGCCTAACTCCATTAAATTTGAAGTAAGTAAGTCCTCTGTCTAGAAGAGTTTGAGTTCTATTGTTTAGTCGAGCATATTAGGGACAAAATGACTGCTTTAATTTTAGGCAAATACTCTATTTTATGAGCAGTACCATCTTAAACCTTTTAAAGTTTAAATATGAAATTTGAATCTTGCATATCACTGTATCCTCAATTGTTTTCAAGTTCAGCTAATCTGTTTCTCATTTGATGCAATTATCTGTTTCTTTTAACCAATTAATTGGGTATAGGCTTCTCTTGTACTTTGTTGTCCTATAACATCTTTTCAGAAGATATTGCTCTTTTTAAACTTTAAAGATACTATTGAACTGTTAATCTATTCCTAATACTCGGTAAATCTTAGGTCATGTATGAGCTTCCTTGTGTGCGTACATAGAAGGTTTAATTGCTGACAGACAGTATTGTCTTTTTCCATGTAGATATTCTGATGCTGCGGAATTGCTGTTGCGATGGGGTCTGGCAGCTGATAAATGCAATGCTGTCCATAGCCAGGGGAAGGTGGGAGCTATCTACTTCTTGAATTTTGTTATATTAATGTGCTACAATTTTTTCCTCTTCATTGAAACCAGCATGTCAAATGAGTTGCCAGGAAGAAAACTGTTAGCTTTCTGTCACATTGATCGTTTTCAGATTTCCTGGTGGATTTATGGGCTAATTTACAGCAGTCAATGAAGTCTGAAAAGACTTTCCAATTGACATAAATGGAAGTTTAATATATATACGTATATATACATTTTTTTTGTTTCTTTTTATTAATCAAGAAAATTTTCGTAGAAAGAGAGCGATCACTAATTTCTATCAGGAAATGGAACCTATTGTGATTTGCTAATAATACATTTATTAATTCCAGGCATATCTTAGTGCCATCATTGTTTACCTTTACGCGAATGACTTCAAACAAGCTGAGAAGTGCTACAATGACTGTTCACAGTGAGTACAAATTCTGAACTATGCCATGGCTTTCTTTTCATCTGCTTCATGGACTTTCATGCTTTATATTACATGATTAAAGTCAAGGAGGGTCATTTTCGAGTTAGCCTTGGGAAGATACTAATTATATACCGAATTTGGATTCACTTGATCTAAGAAGCACAAACATGGACACATTTACTACAAGAAGCGACAATTGCCAATTTCTAAAATACTAAGACATAGACATCTGTTGGGGATACACGATTCTTTAAAATTATTTTTTTAGAATATGTGTGCATTTAAGCTAAAATACTAAATGGATTCCACTAAAAAAGCATAAAATGTCAACTGTCAAGTTCATTAATCATTGTCATCCTAACATTTAACAAATAATAATAAAAAAATATATATGAGTTTCAACTTTGAAGATAGGAAAAAAATGGATTGATGAGTAAAAGGCAGTGGGCAGTAGGTAAAAGAACATATTGCACATACCAACAGGCAGGGCTGGTAGAAACTTTTTCTCCATCGAGAAGAACTGGGAAGGCATTGTTTTTGATGCAGTTACTCGGAAGCAAAACAATGTGGGGGTTGGTTTTTTATACTCTAAAAAGGAAGTTAAATGAGCTTTAACTTTGGGCCCAAATTTTAAACAATTTTCTTCTTTATTTTTTGTTTCAGTGTGTCCGTGAAGTGTCCACTTTGTGTCCGAAATTTTAAAAAAAAAATAATAATAAATATGGCATGCAAATTGGTGCGTTAGACACTTGTCCACTGCATGTTCGACACTGATATCACCTCATATGAATTTTGTGCTTCTCAGCACTTGATAAAAGGAATTGTAAATGAGATCCACTTTTAATAGAATCCTCAGCATCTGTTTCTATAAGTTGATAATTATGTCCTTGAGTTCAAAATAAGATCTTTGACATTAAGAAGTGTCTGCATTGATTATTATCTCGATTATATTGTTTTATAGTTGTACAAGAAGGGACATTTTGCTCAACTCAATACCAATCAAGTCCAGCAAATGATAAATGTCTGTTGTGTAGACTTAGTATTGGACAAAGATAGTAGTCGTGAGTCTTGGGCAAATTGGACAGGAACAAGGTGAAGAGAGTCTTTATAATAAAGACTAAATCAGATGCATGTATATGCCTTCCACTTTCTTTATTTTGCATAAATTAGCCGCACACTGATGATATTTAGTCAGATAGATATGATGCACATATGTGCATTTTCGTATCTTAATCAATACTAAATACATAAAAGCAGCGGAGTAGCCCAATTGATCTTCCCCTAATCTAAAAAACAAGCTAGTTGAGGATAACTCTCCGTGACCACCTGCAGAATGGTTTGCACTCTTCTCCCTTCGTACATGGGCCAAGTGTTATTTTTTGGTTGATTTTTTCTTTCGACTTAGATAAATTCTTAGGAATTTTTAAGCTTCCGAGATATCAAAATAGGCTTTCCGTATCCAAGATAAAACTGGTGATGGATAATCTTTCATTCTTTAGTGTATTTTACTTTGAAAAAAAGGGAATTGTTCCATTTTAATATTTTATTGCAAATGACATCCTGAAAAGGTACTTACAGGTTGAAATTCTTTTATTTCCGCTTTGCAGAGTTGATGCTTTTTCGAGAACTGACCAATACCGTGCTGCTAGTAAGCTATTATCTGCTTATAGGGAAGGCGATGTTGAGGAAATCAAGCGTGTGGCTCAGTCAAGCACTATTTCTCATCTTGATCATGTGGTAAATGTTTTTCCATGCTGTTTTCCATTTCAGTTGATGAAAATGGTTGATTATGAAAGCTTTTCTTATTAACTACAATCAGTGAACCAGAACATCTTTTAATTCTCATGGGAAAAATTCCCCATTTTGAAAAGGAAGAGATAGAAGGGCGAAGAAAATGTAACTTTTCTTTGAAACTTCAACGTGCCGAAGAAAACATGATAATAAAGAAATTAGAACATTGTGTTGAGGGTTTAGGGTTTATGATAATAAAGAAATGTGAACTTTGTGTTGAAACACGGTAAACAAGGACAGGGAGAAAACAACCATCCAAGCATTTTTTCCCTTATAGTTATGTTTTCACTTTGGTATTTGAAACAAGTGAAAGACTTCATTACCTTGACATGTAATTAAATGCAACAATGAGAACATTTCAATTCATGACTCTCCAAATAATTTGCTCAATCGACTTCACTTCGAAATTATGTAAAGAAATGTTAGATGTAGCTGTTGTAGCTTGTAGCATTATTAGTTTTGCTTTGTATTGTTCTGTCATGGAATGTGATTTTTCTACCTACTTTGAACAAAATAAAATTCCTGCAGATTATCAGGCTTGCTAGAAAACTGCCTACAGGTGATGTGAGTGCACTGAAGACTGATACGACTGAAGACCAAGAAGAACCACTGGATGAAAATGATCTCACGTGA

mRNA sequence

ATGAACGGTGCCGAGCTTCTGAGAACAAAACTCAGTATGACAAGATGGAGTGCTGATTGGAAAAGTGCTACTGTCCTCTATGAGCAGGCCGCAAATGCATACCGGGTCCAAAATAACCATGAGAAAGCAAAGATTGCTTTTGAAAAAGCTTCAAAAGGGCAAGAGATGCTCTCCTCACCCTGGGATGCCGCTAAGCATATGGAATCTGCTGGTGCTTTCGCAAAGGAACTTGGTAACTTGACTGAAGTTGCTGACTTTTACAGAAGAGCATCCGAGTTGTATGTTTTGTGTGGAAGATCTCAACCTGCATCTGATGCTTTATCAAAGGGTGCTCATGCTTTGGAAGATAGCAAGCCAGATGAAGCTATCACATTATACACTGAGGCCTGTACACTTCTGGAAGATGATGGCAGGGAACAAATGGCCTTTGATTTATATCGAGATGCCACAAGTGTTTTTGTAAAATTAGAGAAATATTCTGATGCTGCGGAATTGCTGTTGCGATGGGGTCTGGCAGCTGATAAATGCAATGCTGTCCATAGCCAGGGGAAGGCATATCTTAGTGCCATCATTGTTTACCTTTACGCGAATGACTTCAAACAAGCTGAGAAGTGCTACAATGACTGTTCACAAGTTGATGCTTTTTCGAGAACTGACCAATACCGTGCTGCTAGTAAGCTATTATCTGCTTATAGGGAAGGCGATGTTGAGGAAATCAAGCGTGTGGCTCAGTCAAGCACTATTTCTCATCTTGATCATGTGATTATCAGGCTTGCTAGAAAACTGCCTACAGGTGATGTGAGTGCACTGAAGACTGATACGACTGAAGACCAAGAAGAACCACTGGATGAAAATGATCTCACGTGA

Coding sequence (CDS)

ATGAACGGTGCCGAGCTTCTGAGAACAAAACTCAGTATGACAAGATGGAGTGCTGATTGGAAAAGTGCTACTGTCCTCTATGAGCAGGCCGCAAATGCATACCGGGTCCAAAATAACCATGAGAAAGCAAAGATTGCTTTTGAAAAAGCTTCAAAAGGGCAAGAGATGCTCTCCTCACCCTGGGATGCCGCTAAGCATATGGAATCTGCTGGTGCTTTCGCAAAGGAACTTGGTAACTTGACTGAAGTTGCTGACTTTTACAGAAGAGCATCCGAGTTGTATGTTTTGTGTGGAAGATCTCAACCTGCATCTGATGCTTTATCAAAGGGTGCTCATGCTTTGGAAGATAGCAAGCCAGATGAAGCTATCACATTATACACTGAGGCCTGTACACTTCTGGAAGATGATGGCAGGGAACAAATGGCCTTTGATTTATATCGAGATGCCACAAGTGTTTTTGTAAAATTAGAGAAATATTCTGATGCTGCGGAATTGCTGTTGCGATGGGGTCTGGCAGCTGATAAATGCAATGCTGTCCATAGCCAGGGGAAGGCATATCTTAGTGCCATCATTGTTTACCTTTACGCGAATGACTTCAAACAAGCTGAGAAGTGCTACAATGACTGTTCACAAGTTGATGCTTTTTCGAGAACTGACCAATACCGTGCTGCTAGTAAGCTATTATCTGCTTATAGGGAAGGCGATGTTGAGGAAATCAAGCGTGTGGCTCAGTCAAGCACTATTTCTCATCTTGATCATGTGATTATCAGGCTTGCTAGAAAACTGCCTACAGGTGATGTGAGTGCACTGAAGACTGATACGACTGAAGACCAAGAAGAACCACTGGATGAAAATGATCTCACGTGA

Protein sequence

MNGAELLRTKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT*
Homology
BLAST of Chy12G210580 vs. ExPASy Swiss-Prot
Match: Q9SPE5 (Gamma-soluble NSF attachment protein OS=Arabidopsis thaliana OX=3702 GN=GSNAP PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.2e-107
Identity = 193/280 (68.93%), Postives = 239/280 (85.36%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKL++TRWSADW+ AT LYEQAAN +R  N +EKAK+A EKASKGQEM +SPWDAAKHME
Sbjct: 16  TKLTLTRWSADWRGATELYEQAANGFRASNKYEKAKVALEKASKGQEMQASPWDAAKHME 75

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SA A A++L    EVADFYR+ASELYV CGR+QPASDAL K A ALED KPD+AI LYT+
Sbjct: 76  SAAALAQKLSIWNEVADFYRKASELYVECGRAQPASDALGKAARALEDVKPDDAIQLYTD 135

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           AC +LE+DGR+QMAFDLYR   +V++KLEK++DAA   LR G+AADKC+A +SQ KAYLS
Sbjct: 136 ACEILEEDGRDQMAFDLYRACANVYIKLEKFTDAATFFLRLGVAADKCDATNSQCKAYLS 195

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AII+YLYA+D +QAEKCYNDCSQ+DAF ++DQ R+AS+LL+AY EGD+EEIK+VA +ST+
Sbjct: 196 AIILYLYAHDLQQAEKCYNDCSQIDAFLKSDQSRSASRLLTAYNEGDIEEIKKVASASTV 255

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           S+LDH+II+LARKLPTGDV+A++ +T +D    LDE+DLT
Sbjct: 256 SNLDHMIIKLARKLPTGDVTAIQMNTNDD----LDEDDLT 291

BLAST of Chy12G210580 vs. ExPASy Swiss-Prot
Match: Q9U9R7 (Gamma-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snpC PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.1e-31
Identity = 80/254 (31.50%), Postives = 136/254 (53.54%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           +K ++ RW ADW SA + YE+AANA+R    ++ AK  F + S  Q  +   + AAK ME
Sbjct: 24  SKTNIFRWKADWDSAALAYEKAANAFRSAKIYDSAKYCFLRLSLCQTHMDVYYLAAKSME 83

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           +A A AKEL    E A+    + +LY   G S  A+D ++K A  LED   ++ I L T+
Sbjct: 84  NASAMAKELKETQECANLLLESCKLYRTNGNSFQAADTMTKAAKLLEDIDLNQTIKLLTD 143

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           AC L E D ++  + D ++   S+ +K +KY++A +L++       K    H   K+ LS
Sbjct: 144 ACELFELDDKDHFSGDTFKQTISMLLKHKKYTEAVDLMILQNRVFVKLEQNHDLHKSCLS 203

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
            I + L  +D   ++K Y       +F  + +   A +L++A+   DV+ +K++      
Sbjct: 204 VITISLATDDIVASKKYYEQFLDYPSFIHSQEGTTAQELITAFDNHDVDGVKKIVSRHIF 263

Query: 249 SHLDHVIIRLARKL 263
           + LD+ + ++A+ L
Sbjct: 264 NFLDNQVAKIAKNL 277

BLAST of Chy12G210580 vs. ExPASy Swiss-Prot
Match: P81127 (Gamma-soluble NSF attachment protein OS=Bos taurus OX=9913 GN=NAPG PE=1 SV=3)

HSP 1 Score: 100.1 bits (248), Expect = 4.2e-20
Identity = 67/272 (24.63%), Postives = 126/272 (46.32%), Query Frame = 0

Query: 10  KLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHMES 69
           K    +W  D+ SA   Y +AA A++     E+AK A  K +   E   + + AAK  E 
Sbjct: 21  KTGFLKWKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLKEAVAHENNRALFHAAKAYEQ 80

Query: 70  AGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEA 129
           AG   KE+  L E      +AS +Y+  G    A+ AL +    +E+  P++A+ LY + 
Sbjct: 81  AGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIENVDPEKAVQLYQQT 140

Query: 130 CTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSA 189
             + E++ R + A +L   A+ + V+  ++ +AA  + +      +     +  K  ++ 
Sbjct: 141 ANVFENEERLRQAVELLGKASRLLVRGRRFDEAAISIQKEKNIYKEIENYPTCYKKTIAQ 200

Query: 190 IIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTIS 249
           ++V+L+ ND+  AE+C  +   +  F+ ++   A  +LL  Y + D +++  V  S    
Sbjct: 201 VLVHLHRNDYVAAERCVRESYSIPGFNGSEDCAALEQLLEGYDQQDQDQVAEVCNSPLFK 260

Query: 250 HLDHVIIRLARKLPTGDVSALKTDTTEDQEEP 282
           ++D+   +L   L        K      Q +P
Sbjct: 261 YMDNDYAKLGLSLVVPGGGVKKKAAAPPQAKP 292

BLAST of Chy12G210580 vs. ExPASy Swiss-Prot
Match: Q9CWZ7 (Gamma-soluble NSF attachment protein OS=Mus musculus OX=10090 GN=Napg PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.2e-20
Identity = 66/272 (24.26%), Postives = 126/272 (46.32%), Query Frame = 0

Query: 10  KLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHMES 69
           K    +W  D+ SA   Y +AA A++     E+AK A  + +   E   + + AAK  E 
Sbjct: 21  KTGFLKWKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLREAVAHENNRALFHAAKAYEQ 80

Query: 70  AGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEA 129
           AG   KE+  L E      +AS +Y+  G    A+ AL +    +E+  P++A+ LY + 
Sbjct: 81  AGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIENVDPEKAVQLYQQT 140

Query: 130 CTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSA 189
             + E++ R + A +L   A+ + V+  ++ +AA  + +      +     +  K  ++ 
Sbjct: 141 ANVFENEERLRQAVELLGKASRLLVRGRRFDEAALSIQKEKNIYKEIENYPTCYKKTIAQ 200

Query: 190 IIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTIS 249
           ++V+L+ ND+  AE+C  +   +  F+ ++   A  +LL  Y + D +++  V  S    
Sbjct: 201 VLVHLHRNDYVAAERCVRESYSIPGFNGSEDCAALEQLLEGYDQQDQDQVSEVCNSPLFK 260

Query: 250 HLDHVIIRLARKLPTGDVSALKTDTTEDQEEP 282
           ++D+   +L   L        K      Q +P
Sbjct: 261 YMDNDYAKLGLSLVVPGGGIKKKSPATPQAKP 292

BLAST of Chy12G210580 vs. ExPASy Swiss-Prot
Match: Q99747 (Gamma-soluble NSF attachment protein OS=Homo sapiens OX=9606 GN=NAPG PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.2e-19
Identity = 66/272 (24.26%), Postives = 126/272 (46.32%), Query Frame = 0

Query: 10  KLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHMES 69
           K    +W  D+ SA   Y +AA A++     E+AK A  + +   E   + + AAK  E 
Sbjct: 21  KTGFLKWKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLREAVAHENNRALFHAAKAYEQ 80

Query: 70  AGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEA 129
           AG   KE+  L E      +AS +Y+  G    A+ AL +    +E+  P++A+ LY + 
Sbjct: 81  AGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIENVDPEKAVQLYQQT 140

Query: 130 CTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSA 189
             + E++ R + A +L   A+ + V+  ++ +AA  + +      +     +  K  ++ 
Sbjct: 141 ANVFENEERLRQAVELLGKASRLLVRGRRFDEAALSIQKEKNIYKEIENYPTCYKKTIAQ 200

Query: 190 IIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTIS 249
           ++V+L+ ND+  AE+C  +   +  F+ ++   A  +LL  Y + D +++  V  S    
Sbjct: 201 VLVHLHRNDYVAAERCVRESYSIPGFNGSEDCAALEQLLEGYDQQDQDQVSDVCNSPLFK 260

Query: 250 HLDHVIIRLARKLPTGDVSALKTDTTEDQEEP 282
           ++D+   +L   L        K      Q +P
Sbjct: 261 YMDNDYAKLGLSLVVPGGGIKKKSPATPQAKP 292

BLAST of Chy12G210580 vs. ExPASy TrEMBL
Match: A0A0A0LVP4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G554570 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.7e-149
Identity = 275/280 (98.21%), Postives = 278/280 (99.29%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRWSADWKSATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS
Sbjct: 137 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAF+RTDQYRAASKLLSAYREGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           SHLDHVIIRLARKLPTGDVSALKTDTTEDQEE LDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296

BLAST of Chy12G210580 vs. ExPASy TrEMBL
Match: A0A1S3CAN0 (gamma-soluble NSF attachment protein OS=Cucumis melo OX=3656 GN=LOC103498320 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.2e-149
Identity = 273/280 (97.50%), Postives = 279/280 (99.64%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRWSADWKSATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS
Sbjct: 137 ACTLLEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAFSRTDQYRAA+KLLSAYREGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYREGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           SHLDHVIIRLARKLPTGDVSA+KTDTT+DQEEPLDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 296

BLAST of Chy12G210580 vs. ExPASy TrEMBL
Match: A0A5D3BU63 (Gamma-soluble NSF attachment protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1734G00170 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.1e-147
Identity = 269/276 (97.46%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 13  MTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA 72
           MTRWSADWKSATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA
Sbjct: 1   MTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA 60

Query: 73  FAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL 132
            AKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL
Sbjct: 61  LAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL 120

Query: 133 LEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV 192
           LE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV
Sbjct: 121 LEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV 180

Query: 193 YLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTISHLD 252
           YLYA+DFKQAEKCYNDCSQVDAFSRTDQYRAA+KLLSAYREGDVEEIKRVAQSSTISHLD
Sbjct: 181 YLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYREGDVEEIKRVAQSSTISHLD 240

Query: 253 HVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           HVIIRLARKLPTGDVSA+KTDTT+DQEEPLDENDLT
Sbjct: 241 HVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 276

BLAST of Chy12G210580 vs. ExPASy TrEMBL
Match: A0A6J1KJ73 (gamma-soluble NSF attachment protein OS=Cucurbita maxima OX=3661 GN=LOC111495099 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 258/280 (92.14%), Postives = 270/280 (96.43%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRW+ADWK+ATVLYEQAANAYRV+NN E AKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAHALED KPDEAITLYT+
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAHALEDGKPDEAITLYTD 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLAADKCNA HSQGKAYLS
Sbjct: 137 ACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLAADKCNATHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAF RTDQYRAA+KL+SAY EGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLISAYMEGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           SHLDHVIIRLARKLPTGDVSALK D T++ EEPLDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of Chy12G210580 vs. ExPASy TrEMBL
Match: A0A6J1EHZ0 (gamma-soluble NSF attachment protein OS=Cucurbita moschata OX=3662 GN=LOC111434335 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 258/280 (92.14%), Postives = 270/280 (96.43%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRW+ADWK+ATVLYEQAANAYRV+NN E AKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAHALED KPDEAITLYT+
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAHALEDGKPDEAITLYTD 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLAADKCNA HSQGKAYLS
Sbjct: 137 ACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLAADKCNATHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAF RTDQYRAA+KL+SAY EGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLISAYMEGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           SHLDHVIIRLARKLPTGDVSALK D T++ EEPLDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of Chy12G210580 vs. NCBI nr
Match: XP_004135563.1 (gamma-soluble NSF attachment protein [Cucumis sativus] >KGN65970.1 hypothetical protein Csa_023322 [Cucumis sativus])

HSP 1 Score: 543 bits (1400), Expect = 3.06e-194
Identity = 275/280 (98.21%), Postives = 278/280 (99.29%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRWSADWKSATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS
Sbjct: 137 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAF+RTDQYRAASKLLSAYREGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 288
           SHLDHVIIRLARKLPTGDVSALKTDTTEDQEE LDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296

BLAST of Chy12G210580 vs. NCBI nr
Match: XP_008459116.1 (PREDICTED: gamma-soluble NSF attachment protein [Cucumis melo])

HSP 1 Score: 543 bits (1399), Expect = 4.35e-194
Identity = 273/280 (97.50%), Postives = 279/280 (99.64%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRWSADWKSATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS
Sbjct: 137 ACTLLEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAFSRTDQYRAA+KLLSAYREGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYREGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 288
           SHLDHVIIRLARKLPTGDVSA+KTDTT+DQEEPLDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 296

BLAST of Chy12G210580 vs. NCBI nr
Match: KAA0043340.1 (gamma-soluble NSF attachment protein [Cucumis melo var. makuwa] >TYK02670.1 gamma-soluble NSF attachment protein [Cucumis melo var. makuwa])

HSP 1 Score: 536 bits (1381), Expect = 1.15e-191
Identity = 269/276 (97.46%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 13  MTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA 72
           MTRWSADWKSATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA
Sbjct: 1   MTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA 60

Query: 73  FAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL 132
            AKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL
Sbjct: 61  LAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL 120

Query: 133 LEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV 192
           LE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV
Sbjct: 121 LEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV 180

Query: 193 YLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTISHLD 252
           YLYA+DFKQAEKCYNDCSQVDAFSRTDQYRAA+KLLSAYREGDVEEIKRVAQSSTISHLD
Sbjct: 181 YLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYREGDVEEIKRVAQSSTISHLD 240

Query: 253 HVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 288
           HVIIRLARKLPTGDVSA+KTDTT+DQEEPLDENDLT
Sbjct: 241 HVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 276

BLAST of Chy12G210580 vs. NCBI nr
Match: XP_038893768.1 (gamma-soluble NSF attachment protein [Benincasa hispida])

HSP 1 Score: 526 bits (1354), Expect = 3.13e-187
Identity = 264/280 (94.29%), Postives = 271/280 (96.79%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRWSADWK ATVLYEQAANAYRV+NNHEKAKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWSADWKGATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAHALED KPDEAITLYT+
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAHALEDGKPDEAITLYTD 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS
Sbjct: 137 ACTLLEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVD F RTDQYRAASKL+SAY EGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDTFLRTDQYRAASKLISAYIEGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 288
           SHLDHVIIRLARKLPTGDVSALKTD TE+  EPLDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSALKTDATEEHGEPLDENDLT 296

BLAST of Chy12G210580 vs. NCBI nr
Match: XP_022927536.1 (gamma-soluble NSF attachment protein [Cucurbita moschata] >XP_023000740.1 gamma-soluble NSF attachment protein [Cucurbita maxima] >KAG6583818.1 Gamma-soluble NSF attachment protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019444.1 Gamma-soluble NSF attachment protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518 bits (1333), Expect = 4.97e-184
Identity = 258/280 (92.14%), Postives = 270/280 (96.43%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKLSMTRW+ADWK+ATVLYEQAANAYRV+NN E AKIAFEKASKGQEMLSSPWDAAKHME
Sbjct: 17  TKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASKGQEMLSSPWDAAKHME 76

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SAGA AKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAHALED KPDEAITLYT+
Sbjct: 77  SAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAHALEDGKPDEAITLYTD 136

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLAADKCNA HSQGKAYLS
Sbjct: 137 ACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLAADKCNATHSQGKAYLS 196

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AIIVYLYA+DFKQAEKCYNDCSQVDAF RTDQYRAA+KL+SAY EGDVEEIKRVAQSSTI
Sbjct: 197 AIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLISAYMEGDVEEIKRVAQSSTI 256

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 288
           SHLDHVIIRLARKLPTGDVSALK D T++ EEPLDENDLT
Sbjct: 257 SHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of Chy12G210580 vs. TAIR 10
Match: AT4G20410.1 (gamma-soluble NSF attachment protein )

HSP 1 Score: 391.0 bits (1003), Expect = 8.5e-109
Identity = 193/280 (68.93%), Postives = 239/280 (85.36%), Query Frame = 0

Query: 9   TKLSMTRWSADWKSATVLYEQAANAYRVQNNHEKAKIAFEKASKGQEMLSSPWDAAKHME 68
           TKL++TRWSADW+ AT LYEQAAN +R  N +EKAK+A EKASKGQEM +SPWDAAKHME
Sbjct: 16  TKLTLTRWSADWRGATELYEQAANGFRASNKYEKAKVALEKASKGQEMQASPWDAAKHME 75

Query: 69  SAGAFAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTE 128
           SA A A++L    EVADFYR+ASELYV CGR+QPASDAL K A ALED KPD+AI LYT+
Sbjct: 76  SAAALAQKLSIWNEVADFYRKASELYVECGRAQPASDALGKAARALEDVKPDDAIQLYTD 135

Query: 129 ACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLS 188
           AC +LE+DGR+QMAFDLYR   +V++KLEK++DAA   LR G+AADKC+A +SQ KAYLS
Sbjct: 136 ACEILEEDGRDQMAFDLYRACANVYIKLEKFTDAATFFLRLGVAADKCDATNSQCKAYLS 195

Query: 189 AIIVYLYANDFKQAEKCYNDCSQVDAFSRTDQYRAASKLLSAYREGDVEEIKRVAQSSTI 248
           AII+YLYA+D +QAEKCYNDCSQ+DAF ++DQ R+AS+LL+AY EGD+EEIK+VA +ST+
Sbjct: 196 AIILYLYAHDLQQAEKCYNDCSQIDAFLKSDQSRSASRLLTAYNEGDIEEIKKVASASTV 255

Query: 249 SHLDHVIIRLARKLPTGDVSALKTDTTEDQEEPLDENDLT 289
           S+LDH+II+LARKLPTGDV+A++ +T +D    LDE+DLT
Sbjct: 256 SNLDHMIIKLARKLPTGDVTAIQMNTNDD----LDEDDLT 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPE51.2e-10768.93Gamma-soluble NSF attachment protein OS=Arabidopsis thaliana OX=3702 GN=GSNAP PE... [more]
Q9U9R74.1e-3131.50Gamma-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snp... [more]
P811274.2e-2024.63Gamma-soluble NSF attachment protein OS=Bos taurus OX=9913 GN=NAPG PE=1 SV=3[more]
Q9CWZ77.2e-2024.26Gamma-soluble NSF attachment protein OS=Mus musculus OX=10090 GN=Napg PE=1 SV=1[more]
Q997471.2e-1924.26Gamma-soluble NSF attachment protein OS=Homo sapiens OX=9606 GN=NAPG PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVP41.7e-14998.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G554570 PE=3 SV=1[more]
A0A1S3CAN02.2e-14997.50gamma-soluble NSF attachment protein OS=Cucumis melo OX=3656 GN=LOC103498320 PE=... [more]
A0A5D3BU632.1e-14797.46Gamma-soluble NSF attachment protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1KJ731.0e-14192.14gamma-soluble NSF attachment protein OS=Cucurbita maxima OX=3661 GN=LOC111495099... [more]
A0A6J1EHZ01.0e-14192.14gamma-soluble NSF attachment protein OS=Cucurbita moschata OX=3662 GN=LOC1114343... [more]
Match NameE-valueIdentityDescription
XP_004135563.13.06e-19498.21gamma-soluble NSF attachment protein [Cucumis sativus] >KGN65970.1 hypothetical ... [more]
XP_008459116.14.35e-19497.50PREDICTED: gamma-soluble NSF attachment protein [Cucumis melo][more]
KAA0043340.11.15e-19197.46gamma-soluble NSF attachment protein [Cucumis melo var. makuwa] >TYK02670.1 gamm... [more]
XP_038893768.13.13e-18794.29gamma-soluble NSF attachment protein [Benincasa hispida][more]
XP_022927536.14.97e-18492.14gamma-soluble NSF attachment protein [Cucurbita moschata] >XP_023000740.1 gamma-... [more]
Match NameE-valueIdentityDescription
AT4G20410.18.5e-10968.93gamma-soluble NSF attachment protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 24..57
e-value: 48.0
score: 9.0
coord: 183..216
e-value: 23.0
score: 11.8
coord: 103..136
e-value: 110.0
score: 5.8
NoneNo IPR availablePFAMPF14938SNAPcoord: 20..257
e-value: 2.4E-26
score: 92.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 269..288
NoneNo IPR availablePANTHERPTHR13768:SF2GAMMA-SOLUBLE NSF ATTACHMENT PROTEINcoord: 10..278
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 2..264
e-value: 2.9E-75
score: 255.0
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 11..242
IPR000744NSF attachment proteinPANTHERPTHR13768SOLUBLE NSF ATTACHMENT PROTEIN SNAPcoord: 10..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G210580.1Chy12G210580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005483 soluble NSF attachment protein activity
molecular_function GO:0019905 syntaxin binding
molecular_function GO:0005515 protein binding