Chy12G209330 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G209330
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	mitochondrial import inner membrane translocase subunit TIM22-4-like
Location	chrH12: 3563666 .. 3567399 (-)
RNA-Seq Expression	Chy12G209330
Synteny	Chy12G209330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCACTTCTGGAGATGGATCGTCTCTCGATCCACCCACCTCCAGCGACGCCCAGCTGCCTCCAATTCAGCCTGTGAGGATGCCTACAGTTGAAGAGATTCGCGGCCAAGATATTTGGGACAACTGTGCTGTTCGTAGTGTTGTTAGTGGAGTCATGGGTATGTCACCCACTTTGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAAGTTCTCTCCAGCATTCTCACTTACGAACTCTGCTCTGGTTTCTCTCCCCATCCAGCCAAACGAATCTTCGTTGTTTTGATCGATTATAACTGCGATAACTTTCCATCTCTCTCAATATGATACGTATAATTTGGTTTTTAAATGAGATTGGTTTATGTAGTTTGGGTCTGGGTAGTATTTTTAATTGTTTTCAAGTGGATTAAGACTGGGAGGCTTAATCTTTCTCATATAAACATTGTTAGTTTAAACTTCACTAGAACAACCACCGTCTTAATTATCATTTTTTAGTTACTATTAGTTAATTACGTTTCTCCCTCACTATGAGATATATGCAGGTGGTGGGCTTGGATTTTTCGTGGGATTATTTTTGGCGTCGACGGATACTTCAATGGTGCAAGATGAGATGACTGCTAGACAACAGTTTGTTAATACTGCTAAACAGATGGGACGGCGGAGTTGGGGTTCTGCAAAATCGTTTGCAGTCATGGGTTTAATTTTCTCAGCAGCAGAATGTGTTGTAGAAAAGGTATCAATTTCGTGTCTTATGCACAATTGCACATGGTTTTTTATTTTACGGGTTCTAATTCCATTTCGCTGATGTGTGTAGGCTCGAGGTAAACATGATATGACGAATACAATAGTTGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTTGGCCTTCTTACCCTTCCTACTTACCTTATTTTCCAGTCAAGTGTTGCATACATGTGAATTTTCAGCTAGCCATGTAAAGATATTTTTCTGGAAACAATCTTGTGATTATCAATGTGTGATTCTTAACCTTCGAACTTGTGATTCATTACTCTGGTCGAAACTAAAATATTTGTAGGTACCGGTGGTTATCAATGTGTGTGGATTAGTAGTTCAACCGTCTTGCTATTTTATTGTACTTCCATAATCTCTGATTGCATTTTGTTGATATCTCATCTCGTAAGTTGCCGGGGAATAACCTGTTTATTTTTCATGGTTGGCATCGATTGCACTGCTGTGTCTGCATTCTGCACAAGATTGTGCCATTCTTTTTCTTTTCATTTTTTGCTATTTGCTAATATGCTCTAAAATTGCAATTTGAGAGACAAACCTGTGCTCTGTGCAGATTCCTTTCCTGGTCTTGTTCTTTGTTTAAGTCTCCTAGTAAAGTCCAAAGTGAAGCTTTTACCTTCATAGGATATACCTGTCTTTTCCTGAGAAGTTCATCTTTGTTTGTGTAAGCCTCCAAGTAAAGTCCAAAGCGAAGCTGTTACCTTCATTGGATAATCGTGACTTTTCCTGAGAAGTTCATCTTTGTTTGTGTAAGCCTCCAAGTGAAGTCCAAACTGAAACTTTTTCCTTCATTGGGTTCTTGTCATTTCTTTTCCTGATTAACCTTTCTTTGTCAATTCTCATTGCTACTAACATTACAGTTTTTCAGCATCAGAGAAGGATTTTCCATTCCTAAATCTTCCCGTCTTTCAAATTTCCATGCAAATTAAAGAACAGAGAGCTATGTTAGCGAACTGCCTTACAGATACAAGTTTGCTACTAGTTAGATCATACATAGCCTGTGAATGGTGTTTGACAGTGGCTGGTCCACGATTCAAATATCTTAAGAAGTTTAACCTGTTTTCTCTGCCAACGTGTTTAAGGAAATCTTAACTTCTACAAAAAGAATGATTGGTAGAACTTATGAGGAAAGACCACACATGAACATAGGTGAAACACCCATCGAATGAACTACTTAACAATTACCGCAAGAAGGGGGGAGGTTTTAGGTTGGTTCGTAGTAGCTTCCTCTTTGATTTGTATTAGGAGCTCAATTTTTTTGCATGCCATTTTGGCTCATACAACCAAAGACATGTACATTTTTAATGTGGAGAAAATTCTAGGACAGTACGAGGAGAGTTTTGGTAAAGCTTTAGGAGCAATCTCAAGAAAAATTTCTTTAGAACGATATAGGCATGCCTTCTGATCTTCACTTAGGGAATGAGGTATGATTCTTGTTGACTTAACTCTCTTTAAACCTTTGATTATAAAACGTATCCCTGATTTAAAAGATTTGAAGTAGAACCATGTTGGAACTCTATCCTCCAACAAGAGAGCTTAAGAAATATAATCACTGTATTGATAACGTAAAGGATACAAGATAGAAATCGAATCACCAAAAGCAATTCGATTAAACAAACAGGAATTAAACTCACAAACAGAAAGTAAACTCTCACCCTGTAATTCTCTCTTTCAAGAATTTCCAGAAATGCCTTTCTCCCCAAAATACTCAATGCCCTCTCTCTCTCACTTCTTCTTTATAAACTCCCTCTCCCACATTGTGGTCCCCTCCTATTCAGTTACAAAATAACTACCCACTCTCCCCCATACTTTTCTATTTTCTTTTTCCTCCTAAAATAACTAACTCACTCTCCTCCCAGCTTTTCTATTTTCTTTTTCCTCCTACTATACTGATGGATGATTGGTGGTCTAACAAACCAACTGCCATCTAACAGTACACTTCTCGGCTCCTCTTCACTGTTAGCAAGAGCAAAGGCGAATTTATTGACGAGCTATTTTGTTGGGTCCCGTTGGTTGAATATGAATTTGTAACCAAAAACTGAATTGATATTTAGCAATTCAACCAAGTAGTCTGGGGTTTCTAATCCCAGTCGTTCTCAGTTGAAGTAAACAACGAGGGTGGGACTTCCAATGGGTTTTTGGATAACAAGAGATCGTTAAAATTTTATTTTGATCTTATACCTTGGACCTCATTTTATTTTGGTACTTCCAAATGTTTCTATACTTTCAAAAATATTAAATTCAGTTTATATTGTTAGTTTGAAGTTATTTGTTGTTGTTTTGTCAAACATGGTAAAGTAATAATAATAATACTTTTCATTAAGAAAAGTCACGATGTGAATCTGCTTCTAAAATATATAGAGGAAAGAATGAATACCAAAAGGCTTAGAGAGAATGAGTTATGGTCACTATCTACTTAGGATTTAATAGTCGAGATGTGAGCAAGTCGACCCATCTATATGGGATTTAAATAGTAAGACTATTTTTTAAGATTTATTGAATATAAAGGGATTAAATTTAGACCATCCGGAAGTACAGAGAGGGAGTTATATTGACCAAAATGATATTTAAACCAAAATCCTTGAAATTGAGAGATGATTTCTTTTGGTGCCTTCTGTAGAAATCTGAATTGTAGTTTCTGCTTTAATTTTGCATAGCTATTCTGCATCAAACTTGATGACAATCTCTTTGGCCTCCTACCATTCTTTTATCTATTGATCAATGTATGAAGAGTGAATAGGCAAAATAACATAACTGGCTATGTTAACTGAGATTGTCTTTATTTTAACGTTGTCAATTATACGCTTCTCATTTTCATCCCAAGCTGCCACATCTCATCTTATATGGTCTTAATCTCAGGTGGCCCAAAGGCAGCTTGTGCTGGTTGTGCAGGTTTTGCAGCATTCTCAGCGTTGATAGAGAAGTTTTTGGAAAGACATACTTGA

mRNA sequence

ATGGCTTCCACTTCTGGAGATGGATCGTCTCTCGATCCACCCACCTCCAGCGACGCCCAGCTGCCTCCAATTCAGCCTGTGAGGATGCCTACAGTTGAAGAGATTCGCGGCCAAGATATTTGGGACAACTGTGCTGTTCGTAGTGTTGTTAGTGGAGTCATGGGTGGTGGGCTTGGATTTTTCGTGGGATTATTTTTGGCGTCGACGGATACTTCAATGGTGCAAGATGAGATGACTGCTAGACAACAGTTTGTTAATACTGCTAAACAGATGGGACGGCGGAGTTGGGGTTCTGCAAAATCGTTTGCAGTCATGGGTTTAATTTTCTCAGCAGCAGAATGTGTTGTAGAAAAGGCTCGAGGTAAACATGATATGACGAATACAATAGTTGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTGGCCCAAAGGCAGCTTGTGCTGGTTGTGCAGGTTTTGCAGCATTCTCAGCGTTGATAGAGAAGTTTTTGGAAAGACATACTTGA

Coding sequence (CDS)

ATGGCTTCCACTTCTGGAGATGGATCGTCTCTCGATCCACCCACCTCCAGCGACGCCCAGCTGCCTCCAATTCAGCCTGTGAGGATGCCTACAGTTGAAGAGATTCGCGGCCAAGATATTTGGGACAACTGTGCTGTTCGTAGTGTTGTTAGTGGAGTCATGGGTGGTGGGCTTGGATTTTTCGTGGGATTATTTTTGGCGTCGACGGATACTTCAATGGTGCAAGATGAGATGACTGCTAGACAACAGTTTGTTAATACTGCTAAACAGATGGGACGGCGGAGTTGGGGTTCTGCAAAATCGTTTGCAGTCATGGGTTTAATTTTCTCAGCAGCAGAATGTGTTGTAGAAAAGGCTCGAGGTAAACATGATATGACGAATACAATAGTTGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTGGCCCAAAGGCAGCTTGTGCTGGTTGTGCAGGTTTTGCAGCATTCTCAGCGTTGATAGAGAAGTTTTTGGAAAGACATACTTGA

Protein sequence

MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGFFVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT*

Homology

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BLAST of Chy12G209330 vs. ExPASy Swiss-Prot
Match: A2RVP7 (Mitochondrial import inner membrane translocase subunit TIM22-1 OS=Arabidopsis thaliana OX=3702 GN=TIM22-1 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.3e-61
Identity = 112/158 (70.89%), Postives = 130/158 (82.28%), Query Frame = 0


Query: 13  PPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGFFVGLFLASTDTS 72
           PP +SD     IQP+RMPT+EEIR Q++W+NCAVR+V SGVMGGGLG  +GLFL + D  
Sbjct: 16  PPVASDENSTQIQPIRMPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNP 75

Query: 73  MVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAG 132
           +  D MTARQQFV TAKQMG+RSW S K+FAVMGL+FSAAEC+VEKAR KHD  NT +AG
Sbjct: 76  ITHDTMTARQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAG 135

Query: 133 CVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
           CVTGGS+SA+GGPKAAC GCAGFA FS LIEKF +RHT
Sbjct: 136 CVTGGSMSARGGPKAACIGCAGFATFSVLIEKFFDRHT 173

[Close]

BLAST of Chy12G209330 vs. ExPASy Swiss-Prot
Match: A1XJK0 (Mitochondrial import inner membrane translocase subunit TIM22-4 OS=Arabidopsis thaliana OX=3702 GN=TIM22-4 PE=2 SV=3)

HSP 1 Score: 221.9 bits (564), Expect = 5.7e-57
Identity = 104/142 (73.24%), Postives = 120/142 (84.51%), Query Frame = 0


Query: 29  MPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGFFVGLFLASTDTSMVQDEMTARQQFVNTA 88
           MPT+EEIR Q++W+NCAVR+V SGVMGGGLG  +GLFL + D  +  D MTARQQFV TA
Sbjct: 1   MPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNPITHDTMTARQQFVFTA 60

Query: 89  KQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAKGGPKAA 148
           KQMG+RSW S K+FAVMGL+FSAAEC+VEKAR KHD  NT +AGCVTGGS+SA+GGPKAA
Sbjct: 61  KQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAGCVTGGSMSARGGPKAA 120

Query: 149 CAGCAGFAAFSALIEKFLERHT 171
           C GCAGFA FS LIEKF +RHT
Sbjct: 121 CIGCAGFAIFSVLIEKFFDRHT 142

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BLAST of Chy12G209330 vs. ExPASy Swiss-Prot
Match: Q2UAP8 (Mitochondrial import inner membrane translocase subunit tim22 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tim22 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.6e-27
Identity = 61/132 (46.21%), Postives = 88/132 (66.67%), Query Frame = 0


Query: 42  DNCAVRSVVSGVMGGGLGFFVGLFLAS-------TDTSMVQDEMTARQQFVNTAKQMGRR 101
           ++C V++V+SG MG GLG   GLF+AS       T       ++  R+Q     K MG R
Sbjct: 47  ESCPVKTVISGTMGFGLGGVFGLFMASMSYDSTFTPQGKAIMDLPWREQVRRGFKDMGSR 106

Query: 102 SWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAG 161
           SW SAK+F ++G ++S  EC VE  R K+D++N++++GC+TGG + AK GP+AA AGCAG
Sbjct: 107 SWSSAKNFGIVGALYSGTECCVEGLRAKNDLSNSVISGCITGGILGAKAGPQAAAAGCAG 166

Query: 162 FAAFSALIEKFL 167
           FAAFSA I+ ++
Sbjct: 167 FAAFSAAIDAYM 178

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BLAST of Chy12G209330 vs. ExPASy Swiss-Prot
Match: A0A1D8PI78 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=TIM22 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 5.4e-23
Identity = 61/159 (38.36%), Postives = 91/159 (57.23%), Query Frame = 0


Query: 22  PPIQPVRMPTVEEIRGQ------DIWDNCAVRSVVSGVMGGGLGFFVGLFLAS------- 81
           PP +P++  T EE   +         ++C  ++V++GV G  LG F GLF+AS       
Sbjct: 12  PPKKPLQEMTQEEQAEEGARQMIGFMNSCPGKTVMAGVSGFALGGFFGLFMASMAYDTPI 71

Query: 82  -TDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTN 141
            TD      E+  +QQ       M +RS+ SAK+F  +G+++S  EC +E  R KHD+ N
Sbjct: 72  GTDAVKHISELPFKQQMKLQFTDMAKRSYSSAKNFGYIGMVYSGVECTIESLRAKHDIYN 131

Query: 142 TIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFL 167
            + AGC+TG  ++ + GP+AA  GCAGFAAFS  I+ +L
Sbjct: 132 GVSAGCITGAGLAIRAGPQAALVGCAGFAAFSLAIDMYL 170

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BLAST of Chy12G209330 vs. ExPASy Swiss-Prot
Match: Q6BT35 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=TIM22 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-22
Identity = 55/136 (40.44%), Postives = 86/136 (63.24%), Query Frame = 0


Query: 39  DIWDNCAVRSVVSGVMGGGLGFFVGLFLASTD------TSMVQD--EMTARQQFVNTAKQ 98
           ++  +C  ++V++GV G  LG F GLF+AS        T+ V +  ++  +QQ       
Sbjct: 33  ELMQSCPGKTVMAGVSGFFLGGFFGLFMASMSYDVPIGTNAVSNIRDLPFKQQMKLQFSD 92

Query: 99  MGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAKGGPKAACA 158
           MG+R++ SAK+F  +G+++S  EC +E  R KHD+ N + AGC+TGG ++ + GP+AA  
Sbjct: 93  MGKRTYSSAKNFGYIGMVYSGVECAIESLRAKHDIYNGVSAGCITGGGLAIRAGPQAALV 152

Query: 159 GCAGFAAFSALIEKFL 167
           GCAGFAAFS  I+ +L
Sbjct: 153 GCAGFAAFSTAIDLYL 168

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BLAST of Chy12G209330 vs. ExPASy TrEMBL
Match: A0A0A0M0Y0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533580 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 8.5e-88
Identity = 169/170 (99.41%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF
Sbjct: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR
Sbjct: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
            KHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT
Sbjct: 121 AKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170

[Close]

BLAST of Chy12G209330 vs. ExPASy TrEMBL
Match: A0A1S3C5B1 (mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Cucumis melo OX=3656 GN=LOC103497039 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.3e-77
Identity = 148/170 (87.06%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MASTSGDG S DPPTS+DA+ PPI P+RMPTVEEIRGQDIWDNCAVRSVVSGVMGGG+G 
Sbjct: 1   MASTSGDGFSPDPPTSNDAEQPPIAPMRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGIGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL S D S++QDEMTARQQFV TAKQMGRR WGSAKSFAVMG IFSAAECVVEKAR
Sbjct: 61  FLGLFLGSMDNSLMQDEMTARQQFVYTAKQMGRRGWGSAKSFAVMGFIFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
            KHDMTNT+VAGCVTGG+ISAKGGPKAACAGCAGFAAFS LIEKFLERHT
Sbjct: 121 AKHDMTNTVVAGCVTGGAISAKGGPKAACAGCAGFAAFSVLIEKFLERHT 170

[Close]

BLAST of Chy12G209330 vs. ExPASy TrEMBL
Match: A0A6J1K8G7 (mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita maxima OX=3661 GN=LOC111493200 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 9.1e-74
Identity = 139/170 (81.76%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MAS+SGDGSS DP +SS+ + PP++P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL + D  ++QDEMTARQQFV TAKQMGRRSWGSAK+FAVMG +FSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
            KHD+TNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS +IEKFL+RHT
Sbjct: 121 AKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]

BLAST of Chy12G209330 vs. ExPASy TrEMBL
Match: A0A6J1G8S5 (mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita moschata OX=3662 GN=LOC111452007 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 9.1e-74
Identity = 139/170 (81.76%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MAS+SGDGSS DP +SS+ + PP++P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL + D  ++QDEMTARQQFV TAKQMGRRSWGSAK+FAVMG +FSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
            KHD+TNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS +IEKFL+RHT
Sbjct: 121 AKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]

BLAST of Chy12G209330 vs. ExPASy TrEMBL
Match: A0A6J1DKY7 (mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Momordica charantia OX=3673 GN=LOC111022051 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.4e-71
Identity = 135/170 (79.41%), Postives = 156/170 (91.76%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MA+ +G+GSS DP +S++A+ PPI+P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MATNAGNGSSHDPTSSNEAEKPPIEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL + D  ++Q+EMTA+QQF+ TAKQMGRRSWGSAK+FAVMG IFSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQEEMTAKQQFIFTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
            KHD TNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS LIEKFL+RH+
Sbjct: 121 AKHDTTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 170

[Close]

BLAST of Chy12G209330 vs. NCBI nr
Match: XP_004153539.1 (mitochondrial import inner membrane translocase subunit TIM22-4 [Cucumis sativus] >KGN65851.1 hypothetical protein Csa_023190 [Cucumis sativus])

HSP 1 Score: 333 bits (854), Expect = 4.68e-115
Identity = 169/170 (99.41%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF
Sbjct: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR
Sbjct: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170
            KHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT
Sbjct: 121 AKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170

[Close]

BLAST of Chy12G209330 vs. NCBI nr
Match: XP_008457318.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM22-4-like [Cucumis melo])

HSP 1 Score: 298 bits (764), Expect = 2.47e-101
Identity = 148/170 (87.06%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MASTSGDG S DPPTS+DA+ PPI P+RMPTVEEIRGQDIWDNCAVRSVVSGVMGGG+G 
Sbjct: 1   MASTSGDGFSPDPPTSNDAEQPPIAPMRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGIGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL S D S++QDEMTARQQFV TAKQMGRR WGSAKSFAVMG IFSAAECVVEKAR
Sbjct: 61  FLGLFLGSMDNSLMQDEMTARQQFVYTAKQMGRRGWGSAKSFAVMGFIFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170
            KHDMTNT+VAGCVTGG+ISAKGGPKAACAGCAGFAAFS LIEKFLERHT
Sbjct: 121 AKHDMTNTVVAGCVTGGAISAKGGPKAACAGCAGFAAFSVLIEKFLERHT 170

[Close]

BLAST of Chy12G209330 vs. NCBI nr
Match: XP_038893922.1 (mitochondrial import inner membrane translocase subunit TIM22-4-like [Benincasa hispida])

HSP 1 Score: 293 bits (750), Expect = 3.36e-99
Identity = 143/170 (84.12%), Postives = 159/170 (93.53%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MASTSGD SSLDPPTS+D++ PPI+P+RMPT+EEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL + D  ++QDEMTA+QQFV TAKQMGRRSWGSAK+FAVMG IFSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170
            KHDMTNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS LIEKFL+RH+
Sbjct: 121 AKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 170

[Close]

BLAST of Chy12G209330 vs. NCBI nr
Match: XP_022948292.1 (mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita moschata] >XP_022998612.1 mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita maxima] >XP_023523181.1 mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita pepo subsp. pepo] >KAG6607337.1 Mitochondrial import inner membrane translocase subunit TIM22-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 286 bits (733), Expect = 1.31e-96
Identity = 139/170 (81.76%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MAS+SGDGSS DP +SS+ + PP++P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKAR 120
           F+GLFL + D  ++QDEMTARQQFV TAKQMGRRSWGSAK+FAVMG +FSAAECVVEKAR
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKAR 120

Query: 121 GKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170
            KHD+TNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS +IEKFL+RHT
Sbjct: 121 AKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]

BLAST of Chy12G209330 vs. NCBI nr
Match: KAG7037014.1 (Mitochondrial import inner membrane translocase subunit TIM22-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 281 bits (719), Expect = 1.97e-94
Identity = 139/173 (80.35%), Postives = 157/173 (90.75%), Query Frame = 0


Query: 1   MASTSGDGSSLDPPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGF 60
           MAS+SGDGSS DP +SS+ + PP++P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGGLG 
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FVGLFLASTDTSMVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEK-- 120
           F+GLFL + D  ++QDEMTARQQFV TAKQMGRRSWGSAK+FAVMG +FSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120

Query: 121 -ARGKHDMTNTIVAGCVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 170
            AR KHD+TNT+VAGCVTGGSISAKGGPKAACAGCAGFAAFS +IEKFL+RHT
Sbjct: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 173

[Close]

BLAST of Chy12G209330 vs. TAIR 10
Match: AT3G10110.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 237.3 bits (604), Expect = 9.3e-63
Identity = 112/158 (70.89%), Postives = 130/158 (82.28%), Query Frame = 0


Query: 13  PPTSSDAQLPPIQPVRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGFFVGLFLASTDTS 72
           PP +SD     IQP+RMPT+EEIR Q++W+NCAVR+V SGVMGGGLG  +GLFL + D  
Sbjct: 16  PPVASDENSTQIQPIRMPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNP 75

Query: 73  MVQDEMTARQQFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAG 132
           +  D MTARQQFV TAKQMG+RSW S K+FAVMGL+FSAAEC+VEKAR KHD  NT +AG
Sbjct: 76  ITHDTMTARQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAG 135

Query: 133 CVTGGSISAKGGPKAACAGCAGFAAFSALIEKFLERHT 171
           CVTGGS+SA+GGPKAAC GCAGFA FS LIEKF +RHT
Sbjct: 136 CVTGGSMSARGGPKAACIGCAGFATFSVLIEKFFDRHT 173

[Close]

BLAST of Chy12G209330 vs. TAIR 10
Match: AT1G18320.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 221.9 bits (564), Expect = 4.0e-58
Identity = 104/142 (73.24%), Postives = 120/142 (84.51%), Query Frame = 0


Query: 29  MPTVEEIRGQDIWDNCAVRSVVSGVMGGGLGFFVGLFLASTDTSMVQDEMTARQQFVNTA 88
           MPT+EEIR Q++W+NCAVR+V SGVMGGGLG  +GLFL + D  +  D MTARQQFV TA
Sbjct: 1   MPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNPITHDTMTARQQFVFTA 60

Query: 89  KQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAKGGPKAA 148
           KQMG+RSW S K+FAVMGL+FSAAEC+VEKAR KHD  NT +AGCVTGGS+SA+GGPKAA
Sbjct: 61  KQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAGCVTGGSMSARGGPKAA 120

Query: 149 CAGCAGFAAFSALIEKFLERHT 171
           C GCAGFA FS LIEKF +RHT
Sbjct: 121 CIGCAGFAIFSVLIEKFFDRHT 142

[Close]

BLAST of Chy12G209330 vs. TAIR 10
Match: AT2G37410.1 (translocase inner membrane subunit 17-2 )

HSP 1 Score: 42.7 bits (99), Expect = 3.4e-04
Identity = 28/81 (34.57%), Postives = 40/81 (49.38%), Query Frame = 0


Query: 83  QFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAK 142
           +FV   + +   +  +  SFAV G +FS  +C +   R K D  N+I+AG  TGG +S +
Sbjct: 47  RFVGGTQSVSMNAPRTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMR 106

Query: 143 GGPKAACAGCAGFAAFSALIE 164
            G  AA           ALIE
Sbjct: 107 QGAGAASRSAIFGGVLLALIE 127

[Close]

BLAST of Chy12G209330 vs. TAIR 10
Match: AT2G37410.2 (translocase inner membrane subunit 17-2 )

HSP 1 Score: 42.7 bits (99), Expect = 3.4e-04
Identity = 28/81 (34.57%), Postives = 40/81 (49.38%), Query Frame = 0


Query: 83  QFVNTAKQMGRRSWGSAKSFAVMGLIFSAAECVVEKARGKHDMTNTIVAGCVTGGSISAK 142
           +FV   + +   +  +  SFAV G +FS  +C +   R K D  N+I+AG  TGG +S +
Sbjct: 47  RFVGGTQSVSMNAPRTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMR 106

Query: 143 GGPKAACAGCAGFAAFSALIE 164
            G  AA           ALIE
Sbjct: 107 QGAGAASRSAIFGGVLLALIE 127

The following BLAST results are available for this feature:

BLAST of Chy12G209330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2RVP7	1.3e-61	70.89	Mitochondrial import inner membrane translocase subunit TIM22-1 OS=Arabidopsis t...	[more]
A1XJK0	5.7e-57	73.24	Mitochondrial import inner membrane translocase subunit TIM22-4 OS=Arabidopsis t...	[more]
Q2UAP8	3.6e-27	46.21	Mitochondrial import inner membrane translocase subunit tim22 OS=Aspergillus ory...	[more]
A0A1D8PI78	5.4e-23	38.36	Mitochondrial import inner membrane translocase subunit TIM22 OS=Candida albican...	[more]
Q6BT35	1.6e-22	40.44	Mitochondrial import inner membrane translocase subunit TIM22 OS=Debaryomyces ha...	[more]

BLAST of Chy12G209330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0Y0	8.5e-88	99.41	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533580 PE=4 SV=1	[more]
A0A1S3C5B1	2.3e-77	87.06	mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Cucumis ...	[more]
A0A6J1K8G7	9.1e-74	81.76	mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita max...	[more]
A0A6J1G8S5	9.1e-74	81.76	mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita mos...	[more]
A0A6J1DKY7	1.4e-71	79.41	mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Momordic...	[more]

BLAST of Chy12G209330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004153539.1	4.68e-115	99.41	mitochondrial import inner membrane translocase subunit TIM22-4 [Cucumis sativus...	[more]
XP_008457318.1	2.47e-101	87.06	PREDICTED: mitochondrial import inner membrane translocase subunit TIM22-4-like ...	[more]
XP_038893922.1	3.36e-99	84.12	mitochondrial import inner membrane translocase subunit TIM22-4-like [Benincasa ...	[more]
XP_022948292.1	1.31e-96	81.76	mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita mosch...	[more]
KAG7037014.1	1.97e-94	80.35	Mitochondrial import inner membrane translocase subunit TIM22-1 [Cucurbita argyr...	[more]

BLAST of Chy12G209330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10110.1	9.3e-63	70.89	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT1G18320.1	4.0e-58	73.24	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G37410.1	3.4e-04	34.57	translocase inner membrane subunit 17-2	[more]
AT2G37410.2	3.4e-04	34.57	translocase inner membrane subunit 17-2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 44..159 e-value: 1.5E-30 score: 105.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR14110:SF0	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22	coord: 18..169
IPR039175	Mitochondrial import inner membrane translocase subunit TIM22	PANTHER	PTHR14110	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22	coord: 18..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G209330.1	Chy12G209330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0045039	protein insertion into mitochondrial inner membrane
biological_process	GO:0071806	protein transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0042721	TIM22 mitochondrial import inner membrane insertion complex
molecular_function	GO:0030943	mitochondrion targeting sequence binding
molecular_function	GO:0008320	protein transmembrane transporter activity