Chy11G188660 (gene) Cucumber (hystrix) v1

Overview
NameChy11G188660
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationchrH11: 2612105 .. 2615153 (-)
RNA-Seq ExpressionChy11G188660
SyntenyChy11G188660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAATTTCAACTATATCTACCCGTGGTAATCTCGCTGTTGATCGCCGTCGGCATCACCGCAGTCAGTCAAATCAATGGACATGATTTCACGTTTGAAGAAGCAACAATCGAGGAGATCCAGAGAGCTTTTGCCGATGAAAGGCTCACATCGAGAATGTTAGTCGATTTCTATTTAAAACAAATCGAAGCCCTGAATCCGGTGCTTAGGAGCGTGGTGGAGGTGAATCCGGAGGCGAGGGATGAGGCGGACAAGGCGGACCGGCGGCGGAGAGAGGGGAATGTGAAACGGTTGTCGCTTGGAGGACTCGAGGGCGTGCCGGTGCTGGTGAAGGACACTATTGCGACGAAAGATAGGATGAACACGACGGCTGGATCGTACGCATTGGTGGGATCGGTGGTGGCTCGGGACGCTGGCGTGGTTGAGAAGCTGAGGAAAGCTGGGGCGGTGATTTTGGGGAAAGCGAGTCTCACTGAGTGGTACTCGTTTCGGTCCTTGGGTCACGTGCCCAATGGGTGGTGCGCACGTGCCGGTCAAGGAGTGGTGAGTTCTTTCAAACATATTATATACATATATATATATATATTTCTAATTACTTACTAATTTGAGTTTTATTCTCTTTTTGAAAGTGCTAACTTCTAATATAAATTTTAAAATTAATATATTTTTATTGAAATTGTTATTTTTTCAAAATTGTTCCCCAAACGATGAGGTTCGGCTTTCAAATGTATTTGACTCTTACTATTTCTAGGACTATATAAATTTTTAAAAATTTGAAGGGTATGTATTGCAATAATCGTGCGTCAAGGATTGTTTTTTTGCAATTTGCACTATTTTTTACGTCAATAAATATATTAAAAGTTGGTTGATAATTTTCTTTAATTTTCTTTGTCACGTCACCTTTTTTTTTAAAAATAAAATTATTTATGTTAGGTTCTCTTTTTTATTATTATTATTTTGTGGGTAAGGAAAGAAATGTAGATTGAAGCGTAGGTGTACTTAATCATGACTCAAATTCTAATACAAGCTGGTGTTTGTGAAACGATGTAGAATCCTTATTTGGCTTCTGGTGAGACCTGCGGGTCGAGCAGTGGCTCAGCCATTTCTGTTGCTGCTAACATGGTAACCGTCTCACTCGGTACAGAAACTCATGGCTCCATCCTCTGCCCCTCCGATCGAAATTCTGTCGTAGGATTCAAACCAACAGTTGGCCTCACTACCCGAGCTGGAGTCATTCCCATCATGTCTCGCCACGACACCGTCGGGTAAGTCCATACTTAGTCAGAAATGTTCACGAACAAGAGAAAAATCTTAATATTTGAAATTGCATATATATAGGCCTATAACGAGGACAGTGTCGGATGCAGTTTATGTGCTTGATGCAATTGTTGGTTATGATCCAAGAGATGCTGAAGCCACAAGGCAAGGCTCCAAGTTTATACCACAAGGTGGATATAAACAATTTCTAAATCCCAATGGATCGAAAGGGAAAAGAGTTGGAGTTGTTAGAACCCCATTTGCTGATAAGTTCCCTTCAATGCAAGTTTTTGAGAATCATCTTCATACCTTAAGGTAAACAATCTTTGAAAATTAGTTTATGTATAATGGTTTTCATCGTTCATGAGTAAATGTTTGAATTCAATATAAATTTCAAAAAGCTACCTTTGTTTCTTTTATTTTAGTTTTCAAAACTTTGGTTGAGTTTTAAAACCGTTTTTAAAAGTTAATAATGAAACAAATAAATCAAGATGTAGATGTAGCGTGTATAAGCTTAAAATTAAATAGAGTTGTAACAAGGTGTAATTATTTTATTTTATTAAATTTTGTAGGGAAAAAGGTGGTGTTATAGTGGATGATCTTGAAATTGCTGATATAGATATAATATTGAGCTCCAAAAGGAGTGGTGAACTAACAGTTATGTTGGCTGATTTCAAGCTTTTGCTAAATGATTATTTGAAAGAGCTCATAAGTTCCCCAGTCAGATCACTTGCAGATGTTATTGCCTTCAACAACAACCACCCTCAATTGGTGAGAACTTTCTCAATTCTTTTTCAAATATTCACATTCACTCCCAAGTGGTTTTTTTCCGAACAAAAACGAAGTTACTTTCTTCCTCTACTGATCCATGCATGTTAGCTAACTCACTTTAGATTTCTTTGTTTCAAAAAAAAGAAAAAAACAAAGTTTTCTGATCAAATTACTAATGTGTTACTTAATCTCCCTCTGCTTGCCCATGGATGTAGCTAACACATTCTTAATGAAATTCATAAATTCATCGATGTTCGTTGTTTACATGTTGTTCACTTTCTTTGTTTTTCAATTTTATAATAGATTTCTTTATTTTGTTATCGTCTTTTTAAGATGAATCAAGTTTAGAAAATTAGAAAAAAAGATAATTCAAGTGTTTTGTTCGGAATGGTGAAAGGTCTATTTAGATTTGAAAAAAAAAAGATAATTTAAGTGTTCTGTTAGGAATGATGAAAGGTCTATTTAGATTGATTTTTCAAATGCATAAACTCAAAAACCATAACAAATGAGTTAAAAAGAAACAAGTACAAATTTCAAAAACTAAAAATAAAAGACTAAAGAAGGGTTACCAAAGAGCTTAAATACAGTTTGACGTAATGATTTAGTTCTCTTTTGTTCTCTCATGTAGGAGAAAATCAAAGAATATGGACAATCAACTTTCATTCAATCAGAGAAAACAAATGGGTTAGGGGAGAAAGAAAAGAAGGCAATCGAAACGATGGCAAATCTATCACGAAATGGATTTGAGAAGTTAATGAAAGAAAAGGAGTTGGACGCAATCGTCACACCGGGTTCTGGCTGCATTTCAATGCTGGCGATCGGAGGATACCCTGGAATTACTGTCCCAGCAGGATATGACAAAGACGATGGTATGCCATTTGGGATCTGTTTTGGAGGCTTGAAGGGCACAGAGCCAAAGCTTATTGAGATTGCTTATGCTTTTGAGCAAGCCACTATGATGCGAAGGCCTCCATTTTCAAATTCAATAGATTATCAAGTTTCACATAGCAACAGTTGA

mRNA sequence

ATGAAGGAATTTCAACTATATCTACCCGTGGTAATCTCGCTGTTGATCGCCGTCGGCATCACCGCAGTCAGTCAAATCAATGGACATGATTTCACGTTTGAAGAAGCAACAATCGAGGAGATCCAGAGAGCTTTTGCCGATGAAAGGCTCACATCGAGAATGTTAGTCGATTTCTATTTAAAACAAATCGAAGCCCTGAATCCGGTGCTTAGGAGCGTGGTGGAGGTGAATCCGGAGGCGAGGGATGAGGCGGACAAGGCGGACCGGCGGCGGAGAGAGGGGAATGTGAAACGGTTGTCGCTTGGAGGACTCGAGGGCGTGCCGGTGCTGGTGAAGGACACTATTGCGACGAAAGATAGGATGAACACGACGGCTGGATCGTACGCATTGGTGGGATCGGTGGTGGCTCGGGACGCTGGCGTGGTTGAGAAGCTGAGGAAAGCTGGGGCGGTGATTTTGGGGAAAGCGAGTCTCACTGAGTGGTACTCGTTTCGGTCCTTGGGTCACGTGCCCAATGGGTGGTGCGCACGTGCCGGTCAAGGAGTGAATCCTTATTTGGCTTCTGGTGAGACCTGCGGGTCGAGCAGTGGCTCAGCCATTTCTGTTGCTGCTAACATGGTAACCGTCTCACTCGGTACAGAAACTCATGGCTCCATCCTCTGCCCCTCCGATCGAAATTCTGTCGTAGGATTCAAACCAACAGTTGGCCTCACTACCCGAGCTGGAGTCATTCCCATCATGTCTCGCCACGACACCGTCGGGCCTATAACGAGGACAGTGTCGGATGCAGTTTATGTGCTTGATGCAATTGTTGGTTATGATCCAAGAGATGCTGAAGCCACAAGGCAAGGCTCCAAGTTTATACCACAAGGTGGATATAAACAATTTCTAAATCCCAATGGATCGAAAGGGAAAAGAGTTGGAGTTGTTAGAACCCCATTTGCTGATAAGTTCCCTTCAATGCAAGTTTTTGAGAATCATCTTCATACCTTAAGGGAAAAAGGTGGTGTTATAGTGGATGATCTTGAAATTGCTGATATAGATATAATATTGAGCTCCAAAAGGAGTGGTGAACTAACAGTTATGTTGGCTGATTTCAAGCTTTTGCTAAATGATTATTTGAAAGAGCTCATAAGTTCCCCAGTCAGATCACTTGCAGATGTTATTGCCTTCAACAACAACCACCCTCAATTGGAGAAAATCAAAGAATATGGACAATCAACTTTCATTCAATCAGAGAAAACAAATGGGTTAGGGGAGAAAGAAAAGAAGGCAATCGAAACGATGGCAAATCTATCACGAAATGGATTTGAGAAGTTAATGAAAGAAAAGGAGTTGGACGCAATCGTCACACCGGGTTCTGGCTGCATTTCAATGCTGGCGATCGGAGGATACCCTGGAATTACTGTCCCAGCAGGATATGACAAAGACGATGGTATGCCATTTGGGATCTGTTTTGGAGGCTTGAAGGGCACAGAGCCAAAGCTTATTGAGATTGCTTATGCTTTTGAGCAAGCCACTATGATGCGAAGGCCTCCATTTTCAAATTCAATAGATTATCAAGTTTCACATAGCAACAGTTGA

Coding sequence (CDS)

ATGAAGGAATTTCAACTATATCTACCCGTGGTAATCTCGCTGTTGATCGCCGTCGGCATCACCGCAGTCAGTCAAATCAATGGACATGATTTCACGTTTGAAGAAGCAACAATCGAGGAGATCCAGAGAGCTTTTGCCGATGAAAGGCTCACATCGAGAATGTTAGTCGATTTCTATTTAAAACAAATCGAAGCCCTGAATCCGGTGCTTAGGAGCGTGGTGGAGGTGAATCCGGAGGCGAGGGATGAGGCGGACAAGGCGGACCGGCGGCGGAGAGAGGGGAATGTGAAACGGTTGTCGCTTGGAGGACTCGAGGGCGTGCCGGTGCTGGTGAAGGACACTATTGCGACGAAAGATAGGATGAACACGACGGCTGGATCGTACGCATTGGTGGGATCGGTGGTGGCTCGGGACGCTGGCGTGGTTGAGAAGCTGAGGAAAGCTGGGGCGGTGATTTTGGGGAAAGCGAGTCTCACTGAGTGGTACTCGTTTCGGTCCTTGGGTCACGTGCCCAATGGGTGGTGCGCACGTGCCGGTCAAGGAGTGAATCCTTATTTGGCTTCTGGTGAGACCTGCGGGTCGAGCAGTGGCTCAGCCATTTCTGTTGCTGCTAACATGGTAACCGTCTCACTCGGTACAGAAACTCATGGCTCCATCCTCTGCCCCTCCGATCGAAATTCTGTCGTAGGATTCAAACCAACAGTTGGCCTCACTACCCGAGCTGGAGTCATTCCCATCATGTCTCGCCACGACACCGTCGGGCCTATAACGAGGACAGTGTCGGATGCAGTTTATGTGCTTGATGCAATTGTTGGTTATGATCCAAGAGATGCTGAAGCCACAAGGCAAGGCTCCAAGTTTATACCACAAGGTGGATATAAACAATTTCTAAATCCCAATGGATCGAAAGGGAAAAGAGTTGGAGTTGTTAGAACCCCATTTGCTGATAAGTTCCCTTCAATGCAAGTTTTTGAGAATCATCTTCATACCTTAAGGGAAAAAGGTGGTGTTATAGTGGATGATCTTGAAATTGCTGATATAGATATAATATTGAGCTCCAAAAGGAGTGGTGAACTAACAGTTATGTTGGCTGATTTCAAGCTTTTGCTAAATGATTATTTGAAAGAGCTCATAAGTTCCCCAGTCAGATCACTTGCAGATGTTATTGCCTTCAACAACAACCACCCTCAATTGGAGAAAATCAAAGAATATGGACAATCAACTTTCATTCAATCAGAGAAAACAAATGGGTTAGGGGAGAAAGAAAAGAAGGCAATCGAAACGATGGCAAATCTATCACGAAATGGATTTGAGAAGTTAATGAAAGAAAAGGAGTTGGACGCAATCGTCACACCGGGTTCTGGCTGCATTTCAATGCTGGCGATCGGAGGATACCCTGGAATTACTGTCCCAGCAGGATATGACAAAGACGATGGTATGCCATTTGGGATCTGTTTTGGAGGCTTGAAGGGCACAGAGCCAAAGCTTATTGAGATTGCTTATGCTTTTGAGCAAGCCACTATGATGCGAAGGCCTCCATTTTCAAATTCAATAGATTATCAAGTTTCACATAGCAACAGTTGA

Protein sequence

MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPNGSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELTVMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGEKEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGMPFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSNS*
Homology
BLAST of Chy11G188660 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.0e-157
Identity = 293/509 (57.56%), Postives = 389/509 (76.42%), Query Frame = 0

Query: 6   LYLPVVISLLIAVGITAVSQIN-GHDFTFEEATIEEIQRAFADERLTSRMLVDFYLKQIE 65
           L + +++S  + + + + SQI     F+ +EATIE+I+ AF ++RLTS+ LV+ YL+ I 
Sbjct: 12  LLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAIS 71

Query: 66  ALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDRMNTT 125
            LNP+L +V+E NP+A  +A+ ADR R   N  +L +  L GVPVL+KD+I+TKD++NTT
Sbjct: 72  KLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPI--LHGVPVLLKDSISTKDKLNTT 131

Query: 126 AGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVNP 185
           AGS+AL+GSVVARDAGVV++LR++GAVILGKASL+EW  FRS   +P+GW AR  QG NP
Sbjct: 132 AGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFS-IPDGWSARGLQGKNP 191

Query: 186 YLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGVI 245
           Y+ S    GSSSGSAISV AN+V VSLGTET GSIL P+ +NSVVG KP+VGLT+RAGV+
Sbjct: 192 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 251

Query: 246 PIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPNGSKG 305
           PI  R D++GPI RTVSDAV++LDAIVGYDP D EAT+  S+FIP+GGYKQFL  +G KG
Sbjct: 252 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLD-EATKTASEFIPEGGYKQFLTTSGLKG 311

Query: 306 KRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELTVMLA 365
           KR+G+V            + ++H+ TLR +G +++++L I +I++I+    SGE   +LA
Sbjct: 312 KRLGIV-------MKHSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLA 371

Query: 366 DFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGEKEKK 425
           +FK+ LN YLKEL+ SPVRSLADVIA+N    + EK+KE+GQ  F+ +E T+G+GEKEK 
Sbjct: 372 EFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKT 431

Query: 426 AIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGMPFGI 485
           A++ M  LSRNG EKL++E +LDAIVT GS   S+LAIGGYPGI VPAGYD   G+P+GI
Sbjct: 432 ALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYD-SGGVPYGI 491

Query: 486 CFGGLKGTEPKLIEIAYAFEQATMMRRPP 514
            FGGL+ +EPKLIEIA+AFEQAT++R+PP
Sbjct: 492 SFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of Chy11G188660 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 4.6e-73
Identity = 187/517 (36.17%), Postives = 276/517 (53.38%), Query Frame = 0

Query: 30  DFTFEEATIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPEARDEADKADR 89
           + T E+ATI+++Q    +  LTS  +V  YL +   +NP +  ++++NP+    A + D 
Sbjct: 63  NITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDD 122

Query: 90  RRREGNVKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAG 149
            R  G ++    G L G+P +VKD  ATKD+M+TTAGSYAL+GS+V RDA VV++LR+AG
Sbjct: 123 ERANGIIR----GPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 182

Query: 150 AVILGKASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGSAISVAANMVTV 209
           AV+ G A+L+EW   RS      G+ AR GQ   P+  +    GSSSGSAISVA+NM+  
Sbjct: 183 AVLFGHATLSEWADMRS-NDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAF 242

Query: 210 SLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDA 269
           +LGTET GSI+ P+ RN VVG KPTVGLT+R GVIP     DT GPI RTV DAVYV  +
Sbjct: 243 ALGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQS 302

Query: 270 IVGYDPRDAEATRQGSKFIPQGGYKQFL-NPNGSKGKRVGVVRTPFADKFPSMQVFE--- 329
           + G D  D     Q  K    G Y +FL N    +G R G+   P+   + + +  E   
Sbjct: 303 MWGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGL---PWKRLWQNAKADEIDR 362

Query: 330 --NHLHTLREKGGVIVDDLEIADIDII--------LSSKRSGELTVMLADFKLLLNDYLK 389
               +  + E G ++ ++    ++D+I        L S    E TV+  DF   +  YL 
Sbjct: 363 LLEVVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLS 422

Query: 390 ELISSPVRSLADVIAFNNNH-------PQLEKIKEYGQSTFIQSEKTNGL-GEKEKKAIE 449
           E+ ++ + SL D++ +NN +       P +      GQ  F+ S +  G+  E   +A+E
Sbjct: 423 EVKNTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVE 482

Query: 450 TMANLSRN-GFEKLMKEKE--------LDAIVTPGSGCIS--MLAIGGYPGITVPAGYDK 509
            +   S++ G +  +   +        L+ ++ P    I+    A  GYP IT+P G  K
Sbjct: 483 YVRRTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGV-K 542

Query: 510 DDGMPFGICFGGLKGTEPKLIEIAYAFEQATMMRRPP 514
            +G PFG+        EP+LI+   A E     +  P
Sbjct: 543 TNGRPFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

BLAST of Chy11G188660 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.0e-60
Identity = 163/482 (33.82%), Postives = 253/482 (52.49%), Query Frame = 0

Query: 54  MLVDFYLKQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKD 113
           ++V  Y+ +I  +N  +R+V E+NP+A   A + D  R+ G ++    G L G+P+++K+
Sbjct: 107 VVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLR----GPLHGLPIVIKN 166

Query: 114 TIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNG 173
            I T D+M++TAGSYA+ G+  + DA V  KLR+AG VI+GK+  ++W +FRSL    NG
Sbjct: 167 NIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWANFRSLNST-NG 226

Query: 174 WCARAGQGVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKP 233
           W A  GQ    Y+ + +  GSSSGS ++    +   +LGTET GSI+ P+D++++VG KP
Sbjct: 227 WSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPADKSNIVGLKP 286

Query: 234 TVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGY 293
           TVGLT+R  V+PI  R DTVGP+ R+V DA Y+L  I G D  D   +      IP   Y
Sbjct: 287 TVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSAIPFDTIPD--Y 346

Query: 294 KQFLNPNGSKGKRVGVVRTP---FADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDII 353
            +  + N  KGKR+GV R     F      +  F   L  +++ G +IV++ +       
Sbjct: 347 VKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVENTDFTSFAEF 406

Query: 354 LSSKRSGELTVMLADFKLLLNDYLKELISSP--VRSLADVIAFNNNHPQLEKIKEYGQST 413
             S    +  ++ AD    L  + K+L  +P  +  L  +  F  +H    +++EY    
Sbjct: 407 AQSPIPDD--ILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH----RLEEYPSRD 466

Query: 414 FIQSE--KTNGLGEKEKK----AIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAI 473
             + +     G+   + K      + +   +  G    ++  +LDA V P      + A+
Sbjct: 467 TARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLSPYIPAL 526

Query: 474 GGYPGITVPAG---------YDKD-----DGMPFGICFGGLKGTEPKLIEIAYAFEQATM 511
            G P ITVP G         +D++      G+P GI F G   +E KLI +AYAFEQ T 
Sbjct: 527 IGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYAFEQKTH 575

BLAST of Chy11G188660 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.1e-37
Identity = 139/505 (27.52%), Postives = 242/505 (47.92%), Query Frame = 0

Query: 37  TIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPE-ARDEADKADRRRREGN 96
           TI E++       +++  +   YL++I+ + P + ++V +  + A  +A +AD + ++G 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGE 66

Query: 97  VKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGK 156
              L+     G+PV++KD I+T + + TT  S  L   +   +A VVEKL + G +ILGK
Sbjct: 67  DTALT-----GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGK 126

Query: 157 ASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGSAISVAANMVTVSLGTET 216
           ++L E+    ++G        +  +  NP+  S    GSS GSA ++AA+    +LG++T
Sbjct: 127 SNLDEF----AMGSSTENSAFKTTK--NPWDLSRVPGGSSGGSAAAIAADEAAFALGSDT 186

Query: 217 HGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDP 276
            GSI  P+    VVG KPT GL +R G++   S  D +GP T+ V+D   VL+ I+G+DP
Sbjct: 187 GGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDP 246

Query: 277 RDAEATRQGSKFIPQGGYKQFLNPNGSKGKRVGVVRTPFADKFPS--MQVFENHLHTLRE 336
           +D+ + +     I +  Y  +L  +  KG R+GV +  F +       +  +  +  L++
Sbjct: 247 KDSTSLK-----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQD 306

Query: 337 KGGVIVDDLEIADIDIILSSK---RSGELTVMLADFKLLLNDYLKELISSPVRSLADVIA 396
            G  I+ D+ I  ++  L +     S E +  LA +  +   ++ E        L D+  
Sbjct: 307 LGAEII-DISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAE----KYEDLIDMYM 366

Query: 397 FNNNHPQLEKIK-EYGQSTFIQSEKTNGLGEKEKKAIETMANLSRNGFEKLMKEKELDAI 456
              +    +++K      T+  S   +G  +   K    +  L +N FEK    ++ D I
Sbjct: 367 VTRSEGFGKEVKRRIMLGTYALS---SGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVI 426

Query: 457 VTPGSGCISM---------------------LAIGGYPGITVPAGYDKDDGMPFGICFGG 514
           + P S  ++                      + I G PGI++P G    DG+P G+   G
Sbjct: 427 IGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL--SDGLPVGLQIIG 481

BLAST of Chy11G188660 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.4e-37
Identity = 138/505 (27.33%), Postives = 240/505 (47.52%), Query Frame = 0

Query: 37  TIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPE-ARDEADKADRRRREGN 96
           TI E++       +++  +   YL++I+ + P + +++ +  + A   A +AD + + G 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADEKIKNGE 66

Query: 97  VKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGK 156
              L+     G+PV++KD I+T + + TT  S  L   +   +A VVEKL + G +ILGK
Sbjct: 67  DTALT-----GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGK 126

Query: 157 ASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGSAISVAANMVTVSLGTET 216
           ++L E+    ++G        +  +  NP+  S    GSS GSA ++AA+    +LG++T
Sbjct: 127 SNLDEF----AMGSSTENSAFKTTK--NPWDLSRVPGGSSGGSAAAIAADEAAFALGSDT 186

Query: 217 HGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDP 276
            GSI  P+    VVG KPT GL +R G++   S  D +GP T+ V+D   VL+ I+G+DP
Sbjct: 187 GGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDP 246

Query: 277 RDAEATRQGSKFIPQGGYKQFLNPNGSKGKRVGVVRTPFADKFPS--MQVFENHLHTLRE 336
           +D+ + +     I +  Y  +L  +  KG R+GV +  F +       +  +  +  L++
Sbjct: 247 KDSTSLK-----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQD 306

Query: 337 KGGVIVDDLEIADIDIILSSK---RSGELTVMLADFKLLLNDYLKELISSPVRSLADVIA 396
            G  I+ D+ I  ++  L +     S E +  LA +  +   +    I+     L D+  
Sbjct: 307 LGAEII-DISIPYVEYALPAYYIIASAEASSNLARYDGIRYGH----IAGKYEDLIDMYM 366

Query: 397 FNNNHPQLEKIK-EYGQSTFIQSEKTNGLGEKEKKAIETMANLSRNGFEKLMKEKELDAI 456
              +    +++K      T+  S   +G  +   K    +  L +N FEK    ++ D I
Sbjct: 367 VTRSEGFGKEVKRRIMLGTYALS---SGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVI 426

Query: 457 VTPGSGCISM---------------------LAIGGYPGITVPAGYDKDDGMPFGICFGG 514
           + P S  ++                      + I G PGI++P G    DG+P G+   G
Sbjct: 427 IGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL--SDGLPVGLQIIG 481

BLAST of Chy11G188660 vs. ExPASy TrEMBL
Match: A0A0A0LPL7 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264010 PE=4 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.0e-289
Identity = 510/526 (96.96%), Postives = 519/526 (98.67%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           MKEFQL LP VISLLIAVGI+AVSQINGH+FTFEEATIEEIQRAFADERLTSRMLVDFYL
Sbjct: 18  MKEFQLSLPAVISLLIAVGISAVSQINGHNFTFEEATIEEIQRAFADERLTSRMLVDFYL 77

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
           KQIEALNPVLRSVVEVNPEARD+AD+ADRRRREGNVKRLSLGGL+GVPVLVKDTIATKDR
Sbjct: 78  KQIEALNPVLRSVVEVNPEARDDADRADRRRREGNVKRLSLGGLDGVPVLVKDTIATKDR 137

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCAR+GQ
Sbjct: 138 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARSGQ 197

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
           GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR
Sbjct: 198 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 257

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITRTVSDAVYVLDAIVGYDPRDAE TRQGSKFIPQGGYKQFLNPN
Sbjct: 258 AGVIPIMSHHDTVGPITRTVSDAVYVLDAIVGYDPRDAEVTRQGSKFIPQGGYKQFLNPN 317

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           GSKGKR+GVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADID ILSSKRSGELT
Sbjct: 318 GSKGKRIGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDTILSSKRSGELT 377

Query: 361 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 420
           VMLADFKLLLNDYLKELISSPVRSLAD+IAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE
Sbjct: 378 VMLADFKLLLNDYLKELISSPVRSLADIIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 437

Query: 421 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 480
           KEKKAIETMANLSRNGFEKLMKE ELD IVTPGSGCIS+LAIGGYPGITVPAGYDKDDGM
Sbjct: 438 KEKKAIETMANLSRNGFEKLMKENELDVIVTPGSGCISVLAIGGYPGITVPAGYDKDDGM 497

Query: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 527
           PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN
Sbjct: 498 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 543

BLAST of Chy11G188660 vs. ExPASy TrEMBL
Match: A0A5D3BIC8 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001400 PE=4 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.2e-281
Identity = 499/526 (94.87%), Postives = 512/526 (97.34%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           MKEFQL LP VISLLIAVGI+A+SQINGHDFT EEATIEEIQRAFADERLTSRMLVDFYL
Sbjct: 1   MKEFQLSLPAVISLLIAVGISAISQINGHDFTIEEATIEEIQRAFADERLTSRMLVDFYL 60

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
           KQIEALNPVLRSVVEVNPEARDEADKADRRRR+GNVKRLSLGGL+GVPVLVKDTIATKDR
Sbjct: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRRDGNVKRLSLGGLDGVPVLVKDTIATKDR 120

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASL+EWYSFRSLGHVPNGWCARAGQ
Sbjct: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLSEWYSFRSLGHVPNGWCARAGQ 180

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
            VNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR
Sbjct: 181 AVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITRTVSDAVYVLDAIVGYDPRDAEAT +GSKFIP GGYKQFLNPN
Sbjct: 241 AGVIPIMSSHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATSEGSKFIPLGGYKQFLNPN 300

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           GSKGKR+GVVRTPFADKFPSMQVFENHLHTLR+KGGVIVDDLEIADIDIILS KRSGELT
Sbjct: 301 GSKGKRIGVVRTPFADKFPSMQVFENHLHTLRDKGGVIVDDLEIADIDIILSPKRSGELT 360

Query: 361 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 420
           VMLADFKLLLNDYLKEL+SSPVRSLADVIAFNNNH QLEKIKEYGQSTFIQSEKTNGLGE
Sbjct: 361 VMLADFKLLLNDYLKELLSSPVRSLADVIAFNNNHSQLEKIKEYGQSTFIQSEKTNGLGE 420

Query: 421 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 480
           KEKKAIETMANLSRNGFEKLM+E ELDAIVTPGSGC S+LAIGGYPGITVPAGY++DDGM
Sbjct: 421 KEKKAIETMANLSRNGFEKLMEENELDAIVTPGSGCASVLAIGGYPGITVPAGYNEDDGM 480

Query: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 527
           PFGICFGGLKGTE KLIEIAYAFEQATMMRRPPF NSID QVSHSN
Sbjct: 481 PFGICFGGLKGTEAKLIEIAYAFEQATMMRRPPFPNSIDCQVSHSN 526

BLAST of Chy11G188660 vs. ExPASy TrEMBL
Match: A0A1S3C5Z2 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497305 PE=4 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.2e-281
Identity = 499/526 (94.87%), Postives = 512/526 (97.34%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           MKEFQL LP VISLLIAVGI+A+SQINGHDFT EEATIEEIQRAFADERLTSRMLVDFYL
Sbjct: 1   MKEFQLSLPAVISLLIAVGISAISQINGHDFTIEEATIEEIQRAFADERLTSRMLVDFYL 60

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
           KQIEALNPVLRSVVEVNPEARDEADKADRRRR+GNVKRLSLGGL+GVPVLVKDTIATKDR
Sbjct: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRRDGNVKRLSLGGLDGVPVLVKDTIATKDR 120

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASL+EWYSFRSLGHVPNGWCARAGQ
Sbjct: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLSEWYSFRSLGHVPNGWCARAGQ 180

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
            VNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR
Sbjct: 181 AVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITRTVSDAVYVLDAIVGYDPRDAEAT +GSKFIP GGYKQFLNPN
Sbjct: 241 AGVIPIMSSHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATSEGSKFIPLGGYKQFLNPN 300

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           GSKGKR+GVVRTPFADKFPSMQVFENHLHTLR+KGGVIVDDLEIADIDIILS KRSGELT
Sbjct: 301 GSKGKRIGVVRTPFADKFPSMQVFENHLHTLRDKGGVIVDDLEIADIDIILSPKRSGELT 360

Query: 361 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 420
           VMLADFKLLLNDYLKEL+SSPVRSLADVIAFNNNH QLEKIKEYGQSTFIQSEKTNGLGE
Sbjct: 361 VMLADFKLLLNDYLKELLSSPVRSLADVIAFNNNHSQLEKIKEYGQSTFIQSEKTNGLGE 420

Query: 421 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 480
           KEKKAIETMANLSRNGFEKLM+E ELDAIVTPGSGC S+LAIGGYPGITVPAGY++DDGM
Sbjct: 421 KEKKAIETMANLSRNGFEKLMEENELDAIVTPGSGCASVLAIGGYPGITVPAGYNEDDGM 480

Query: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 527
           PFGICFGGLKGTE KLIEIAYAFEQATMMRRPPF NSID QVSHSN
Sbjct: 481 PFGICFGGLKGTEAKLIEIAYAFEQATMMRRPPFPNSIDCQVSHSN 526

BLAST of Chy11G188660 vs. ExPASy TrEMBL
Match: A0A6J1CX64 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015398 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.1e-238
Identity = 420/505 (83.17%), Postives = 464/505 (91.88%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           M+E +L +P  I+LLI V + + S+I G DFT +EATIEEIQRAFAD++LTSR LVDFYL
Sbjct: 1   MQELRLPVPAFIALLI-VAVGSFSRIEGQDFTIQEATIEEIQRAFADQKLTSRRLVDFYL 60

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
            +IEALNPVLRSVVEVNPEARDEAD+ADRRRRE +  R  +G L+G+PVLVKD IATKD+
Sbjct: 61  SRIEALNPVLRSVVEVNPEARDEADEADRRRREADENRSPVGALDGIPVLVKDGIATKDK 120

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           +NTTAGSYALVGS VARD GVVEKLR+AGAVILGK+S++EWYSFR+LGHVPNGWCARAGQ
Sbjct: 121 LNTTAGSYALVGSAVARDGGVVEKLRRAGAVILGKSSMSEWYSFRALGHVPNGWCARAGQ 180

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
           GVNPY+ASGETCGSSSGSAISVAANMVTVSLGTET GSILCPSDRNSVVGFKPTVGLT+R
Sbjct: 181 GVNPYVASGETCGSSSGSAISVAANMVTVSLGTETRGSILCPSDRNSVVGFKPTVGLTSR 240

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITR+VSDAVYVLDAI G+DPRDAEAT QGSKFIP+GGYKQFLNPN
Sbjct: 241 AGVIPIMSHHDTVGPITRSVSDAVYVLDAIAGFDPRDAEATSQGSKFIPEGGYKQFLNPN 300

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           G+KGKR+GVVR PFADKFPSMQVFENHLHTLR++G VIVD LEI DIDIILS KRSG LT
Sbjct: 301 GAKGKRIGVVRNPFADKFPSMQVFENHLHTLRQRGAVIVDHLEIVDIDIILSPKRSGLLT 360

Query: 361 VMLADFKLLLNDYL-KELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLG 420
           VMLADFK+LLNDYL KELI+SPVRSLADVIAFNN HP++EK+KEYGQ+TFI+SEKTNG G
Sbjct: 361 VMLADFKMLLNDYLMKELITSPVRSLADVIAFNNKHPEMEKLKEYGQATFIESEKTNGFG 420

Query: 421 EKEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDG 480
           EKEKKA+E MANLSRNGFEKLM+E  LDAIVTPG GCIS+LAIGGYPGITVPAGY + DG
Sbjct: 421 EKEKKAVEMMANLSRNGFEKLMRENALDAIVTPGDGCISVLAIGGYPGITVPAGYGEQDG 480

Query: 481 MPFGICFGGLKGTEPKLIEIAYAFE 505
           MPFG+CFGGLKGTEPKLIEIAYAFE
Sbjct: 481 MPFGMCFGGLKGTEPKLIEIAYAFE 504

BLAST of Chy11G188660 vs. ExPASy TrEMBL
Match: A0A7N2N033 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 2.0e-212
Identity = 378/519 (72.83%), Postives = 441/519 (84.97%), Query Frame = 0

Query: 6   LYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYLKQIEA 65
           L+ P++  L+I   +  +S  +GHDF  EEA+IEEIQRAF + RLTSR LVDFYL +IE 
Sbjct: 12  LFAPLISLLIITSNL--ISTTDGHDFKIEEASIEEIQRAFTENRLTSRQLVDFYLDRIEE 71

Query: 66  LNPVLRSVVEVNPEARDEADKADRRRREGNVKRL--SLGGLEGVPVLVKDTIATKDRMNT 125
           LNP+LRSVVEVNP+ARD+AD ADR R     + +  +LG L G+PVL+KDTIA+KD++NT
Sbjct: 72  LNPLLRSVVEVNPDARDQADVADRERGVNGDRSMVAALGELHGIPVLLKDTIASKDKLNT 131

Query: 126 TAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVN 185
           TAGSYAL+GSVVARDAGVVEKLRK GAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVN
Sbjct: 132 TAGSYALLGSVVARDAGVVEKLRKGGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVN 191

Query: 186 PYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGV 245
           PY+ SG  CGSSSGSAISVAANMV VSL +ETHGSILCP+D NSVVGFKPTVGLT+RAGV
Sbjct: 192 PYVPSGNPCGSSSGSAISVAANMVAVSLASETHGSILCPADHNSVVGFKPTVGLTSRAGV 251

Query: 246 IPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPNGSK 305
           IPI+ RHDTVG I+RTVSDAVYVLD I G+DP+D EAT++ +KF+P GGYKQFLNPNG +
Sbjct: 252 IPILPRHDTVGTISRTVSDAVYVLDVIAGFDPQDYEATKEAAKFVPVGGYKQFLNPNGLE 311

Query: 306 GKRVGVVRTPFA---DKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 365
           GKR+GVVR PF    +K   ++ FE HL T+R++G  + D+LEIA++D+I SSKRSGELT
Sbjct: 312 GKRLGVVRNPFVSSLNKSSVIEEFERHLRTIRQRGATVADNLEIANVDVIQSSKRSGELT 371

Query: 366 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 425
            MLA+FKL  NDYLK+LISSPVRSLAD+IAFN N+P LEK KEYGQ+TFI SEKT+G+GE
Sbjct: 372 AMLAEFKLSFNDYLKDLISSPVRSLADIIAFNQNNPDLEKTKEYGQATFIASEKTSGIGE 431

Query: 426 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 485
           KE++A E M NLSRNGFEKLM E ELDA+VTPG+G I +LAIGG+PGITVPAGYD   GM
Sbjct: 432 KERQAFELMENLSRNGFEKLMMENELDALVTPGTGAIPLLAIGGHPGITVPAGYD-SGGM 491

Query: 486 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSID 520
           PFGICFGG+KGTEPKLIEIAYAFEQATM+RRPPFS S +
Sbjct: 492 PFGICFGGIKGTEPKLIEIAYAFEQATMIRRPPFSKSFE 527

BLAST of Chy11G188660 vs. NCBI nr
Match: XP_004147067.2 (probable amidase At4g34880 [Cucumis sativus] >KGN61906.1 hypothetical protein Csa_006769 [Cucumis sativus])

HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 510/526 (96.96%), Postives = 519/526 (98.67%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           MKEFQL LP VISLLIAVGI+AVSQINGH+FTFEEATIEEIQRAFADERLTSRMLVDFYL
Sbjct: 18  MKEFQLSLPAVISLLIAVGISAVSQINGHNFTFEEATIEEIQRAFADERLTSRMLVDFYL 77

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
           KQIEALNPVLRSVVEVNPEARD+AD+ADRRRREGNVKRLSLGGL+GVPVLVKDTIATKDR
Sbjct: 78  KQIEALNPVLRSVVEVNPEARDDADRADRRRREGNVKRLSLGGLDGVPVLVKDTIATKDR 137

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCAR+GQ
Sbjct: 138 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARSGQ 197

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
           GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR
Sbjct: 198 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 257

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITRTVSDAVYVLDAIVGYDPRDAE TRQGSKFIPQGGYKQFLNPN
Sbjct: 258 AGVIPIMSHHDTVGPITRTVSDAVYVLDAIVGYDPRDAEVTRQGSKFIPQGGYKQFLNPN 317

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           GSKGKR+GVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADID ILSSKRSGELT
Sbjct: 318 GSKGKRIGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDTILSSKRSGELT 377

Query: 361 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 420
           VMLADFKLLLNDYLKELISSPVRSLAD+IAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE
Sbjct: 378 VMLADFKLLLNDYLKELISSPVRSLADIIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 437

Query: 421 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 480
           KEKKAIETMANLSRNGFEKLMKE ELD IVTPGSGCIS+LAIGGYPGITVPAGYDKDDGM
Sbjct: 438 KEKKAIETMANLSRNGFEKLMKENELDVIVTPGSGCISVLAIGGYPGITVPAGYDKDDGM 497

Query: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 526
           PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN
Sbjct: 498 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 543

BLAST of Chy11G188660 vs. NCBI nr
Match: XP_008457657.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >KAA0045710.1 putative amidase [Cucumis melo var. makuwa] >TYJ99570.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 499/526 (94.87%), Postives = 512/526 (97.34%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           MKEFQL LP VISLLIAVGI+A+SQINGHDFT EEATIEEIQRAFADERLTSRMLVDFYL
Sbjct: 1   MKEFQLSLPAVISLLIAVGISAISQINGHDFTIEEATIEEIQRAFADERLTSRMLVDFYL 60

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
           KQIEALNPVLRSVVEVNPEARDEADKADRRRR+GNVKRLSLGGL+GVPVLVKDTIATKDR
Sbjct: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRRDGNVKRLSLGGLDGVPVLVKDTIATKDR 120

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASL+EWYSFRSLGHVPNGWCARAGQ
Sbjct: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLSEWYSFRSLGHVPNGWCARAGQ 180

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
            VNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR
Sbjct: 181 AVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITRTVSDAVYVLDAIVGYDPRDAEAT +GSKFIP GGYKQFLNPN
Sbjct: 241 AGVIPIMSSHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATSEGSKFIPLGGYKQFLNPN 300

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           GSKGKR+GVVRTPFADKFPSMQVFENHLHTLR+KGGVIVDDLEIADIDIILS KRSGELT
Sbjct: 301 GSKGKRIGVVRTPFADKFPSMQVFENHLHTLRDKGGVIVDDLEIADIDIILSPKRSGELT 360

Query: 361 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 420
           VMLADFKLLLNDYLKEL+SSPVRSLADVIAFNNNH QLEKIKEYGQSTFIQSEKTNGLGE
Sbjct: 361 VMLADFKLLLNDYLKELLSSPVRSLADVIAFNNNHSQLEKIKEYGQSTFIQSEKTNGLGE 420

Query: 421 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 480
           KEKKAIETMANLSRNGFEKLM+E ELDAIVTPGSGC S+LAIGGYPGITVPAGY++DDGM
Sbjct: 421 KEKKAIETMANLSRNGFEKLMEENELDAIVTPGSGCASVLAIGGYPGITVPAGYNEDDGM 480

Query: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHSN 526
           PFGICFGGLKGTE KLIEIAYAFEQATMMRRPPF NSID QVSHSN
Sbjct: 481 PFGICFGGLKGTEAKLIEIAYAFEQATMMRRPPFPNSIDCQVSHSN 526

BLAST of Chy11G188660 vs. NCBI nr
Match: XP_038902577.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 467/525 (88.95%), Postives = 491/525 (93.52%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           MKEFQL LP +ISLLI VG  A+SQIN HDFT EEATIEEIQRAFADERLTSRMLVDFYL
Sbjct: 1   MKEFQLPLPALISLLIVVGSGAISQINAHDFTIEEATIEEIQRAFADERLTSRMLVDFYL 60

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
           KQIEAL P+LRSVVEVNPEARDEAD+ADRR RE NV  L +GGL+GVPVLVKDTI TKD+
Sbjct: 61  KQIEALTPMLRSVVEVNPEARDEADEADRRWREANVNWLPVGGLDGVPVLVKDTIVTKDK 120

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASL+EWYSFR+LG VPNGWCARAGQ
Sbjct: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLSEWYSFRALG-VPNGWCARAGQ 180

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
           GVNPY+ASGETCGSSSGSAISVAANMV VSLGTETHGSILCPSD NSVVGFKPTVGLTTR
Sbjct: 181 GVNPYVASGETCGSSSGSAISVAANMVAVSLGTETHGSILCPSDHNSVVGFKPTVGLTTR 240

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMSRHDTVGPI RTVSDAVYVLDAIVG+D RDAEAT QGSKFIPQGGYKQFLNPN
Sbjct: 241 AGVIPIMSRHDTVGPIARTVSDAVYVLDAIVGFDSRDAEATSQGSKFIPQGGYKQFLNPN 300

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           GS GKR+GVVR PFADKF SMQ FENHLHTLR+KG VIVD LEIADIDIILSSKRSGELT
Sbjct: 301 GSSGKRIGVVRNPFADKFHSMQAFENHLHTLRQKGAVIVDHLEIADIDIILSSKRSGELT 360

Query: 361 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 420
           VMLA+FKLLLNDYLKELI SPVRSLADVIAFNN H +LEKIKEYGQ+TFIQS+KTNG G+
Sbjct: 361 VMLAEFKLLLNDYLKELIGSPVRSLADVIAFNNKHAELEKIKEYGQATFIQSKKTNGFGK 420

Query: 421 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 480
           KEKKAIETMANLSRNGFEKLM+E ELDAIVT GSGC+S+LAIGGYPGITVPAGY KDDG+
Sbjct: 421 KEKKAIETMANLSRNGFEKLMRENELDAIVTLGSGCVSVLAIGGYPGITVPAGYGKDDGL 480

Query: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSIDYQVSHS 525
           PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPF NS+DYQ+SHS
Sbjct: 481 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFHNSMDYQISHS 524

BLAST of Chy11G188660 vs. NCBI nr
Match: XP_022146104.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 827 bits (2136), Expect = 7.26e-299
Identity = 420/505 (83.17%), Postives = 464/505 (91.88%), Query Frame = 0

Query: 1   MKEFQLYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYL 60
           M+E +L +P  I+LLI V + + S+I G DFT +EATIEEIQRAFAD++LTSR LVDFYL
Sbjct: 1   MQELRLPVPAFIALLI-VAVGSFSRIEGQDFTIQEATIEEIQRAFADQKLTSRRLVDFYL 60

Query: 61  KQIEALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDR 120
            +IEALNPVLRSVVEVNPEARDEAD+ADRRRRE +  R  +G L+G+PVLVKD IATKD+
Sbjct: 61  SRIEALNPVLRSVVEVNPEARDEADEADRRRREADENRSPVGALDGIPVLVKDGIATKDK 120

Query: 121 MNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQ 180
           +NTTAGSYALVGS VARD GVVEKLR+AGAVILGK+S++EWYSFR+LGHVPNGWCARAGQ
Sbjct: 121 LNTTAGSYALVGSAVARDGGVVEKLRRAGAVILGKSSMSEWYSFRALGHVPNGWCARAGQ 180

Query: 181 GVNPYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTR 240
           GVNPY+ASGETCGSSSGSAISVAANMVTVSLGTET GSILCPSDRNSVVGFKPTVGLT+R
Sbjct: 181 GVNPYVASGETCGSSSGSAISVAANMVTVSLGTETRGSILCPSDRNSVVGFKPTVGLTSR 240

Query: 241 AGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPN 300
           AGVIPIMS HDTVGPITR+VSDAVYVLDAI G+DPRDAEAT QGSKFIP+GGYKQFLNPN
Sbjct: 241 AGVIPIMSHHDTVGPITRSVSDAVYVLDAIAGFDPRDAEATSQGSKFIPEGGYKQFLNPN 300

Query: 301 GSKGKRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 360
           G+KGKR+GVVR PFADKFPSMQVFENHLHTLR++G VIVD LEI DIDIILS KRSG LT
Sbjct: 301 GAKGKRIGVVRNPFADKFPSMQVFENHLHTLRQRGAVIVDHLEIVDIDIILSPKRSGLLT 360

Query: 361 VMLADFKLLLNDYL-KELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLG 420
           VMLADFK+LLNDYL KELI+SPVRSLADVIAFNN HP++EK+KEYGQ+TFI+SEKTNG G
Sbjct: 361 VMLADFKMLLNDYLMKELITSPVRSLADVIAFNNKHPEMEKLKEYGQATFIESEKTNGFG 420

Query: 421 EKEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDG 480
           EKEKKA+E MANLSRNGFEKLM+E  LDAIVTPG GCIS+LAIGGYPGITVPAGY + DG
Sbjct: 421 EKEKKAVEMMANLSRNGFEKLMRENALDAIVTPGDGCISVLAIGGYPGITVPAGYGEQDG 480

Query: 481 MPFGICFGGLKGTEPKLIEIAYAFE 504
           MPFG+CFGGLKGTEPKLIEIAYAFE
Sbjct: 481 MPFGMCFGGLKGTEPKLIEIAYAFE 504

BLAST of Chy11G188660 vs. NCBI nr
Match: XP_030944982.1 (probable amidase At4g34880 [Quercus lobata])

HSP 1 Score: 740 bits (1911), Expect = 4.23e-264
Identity = 378/519 (72.83%), Postives = 441/519 (84.97%), Query Frame = 0

Query: 6   LYLPVVISLLIAVGITAVSQINGHDFTFEEATIEEIQRAFADERLTSRMLVDFYLKQIEA 65
           L+ P++  L+I   +  +S  +GHDF  EEA+IEEIQRAF + RLTSR LVDFYL +IE 
Sbjct: 12  LFAPLISLLIITSNL--ISTTDGHDFKIEEASIEEIQRAFTENRLTSRQLVDFYLDRIEE 71

Query: 66  LNPVLRSVVEVNPEARDEADKADRRRREGNVKRL--SLGGLEGVPVLVKDTIATKDRMNT 125
           LNP+LRSVVEVNP+ARD+AD ADR R     + +  +LG L G+PVL+KDTIA+KD++NT
Sbjct: 72  LNPLLRSVVEVNPDARDQADVADRERGVNGDRSMVAALGELHGIPVLLKDTIASKDKLNT 131

Query: 126 TAGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVN 185
           TAGSYAL+GSVVARDAGVVEKLRK GAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVN
Sbjct: 132 TAGSYALLGSVVARDAGVVEKLRKGGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVN 191

Query: 186 PYLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGV 245
           PY+ SG  CGSSSGSAISVAANMV VSL +ETHGSILCP+D NSVVGFKPTVGLT+RAGV
Sbjct: 192 PYVPSGNPCGSSSGSAISVAANMVAVSLASETHGSILCPADHNSVVGFKPTVGLTSRAGV 251

Query: 246 IPIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPNGSK 305
           IPI+ RHDTVG I+RTVSDAVYVLD I G+DP+D EAT++ +KF+P GGYKQFLNPNG +
Sbjct: 252 IPILPRHDTVGTISRTVSDAVYVLDVIAGFDPQDYEATKEAAKFVPVGGYKQFLNPNGLE 311

Query: 306 GKRVGVVRTPFA---DKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELT 365
           GKR+GVVR PF    +K   ++ FE HL T+R++G  + D+LEIA++D+I SSKRSGELT
Sbjct: 312 GKRLGVVRNPFVSSLNKSSVIEEFERHLRTIRQRGATVADNLEIANVDVIQSSKRSGELT 371

Query: 366 VMLADFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGE 425
            MLA+FKL  NDYLK+LISSPVRSLAD+IAFN N+P LEK KEYGQ+TFI SEKT+G+GE
Sbjct: 372 AMLAEFKLSFNDYLKDLISSPVRSLADIIAFNQNNPDLEKTKEYGQATFIASEKTSGIGE 431

Query: 426 KEKKAIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGM 485
           KE++A E M NLSRNGFEKLM E ELDA+VTPG+G I +LAIGG+PGITVPAGYD   GM
Sbjct: 432 KERQAFELMENLSRNGFEKLMMENELDALVTPGTGAIPLLAIGGHPGITVPAGYDSG-GM 491

Query: 486 PFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNSID 519
           PFGICFGG+KGTEPKLIEIAYAFEQATM+RRPPFS S +
Sbjct: 492 PFGICFGGIKGTEPKLIEIAYAFEQATMIRRPPFSKSFE 527

BLAST of Chy11G188660 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 482.6 bits (1241), Expect = 3.9e-136
Identity = 263/509 (51.67%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 6   LYLPVVISLLIAVGITAVSQIN-GHDFTFEEATIEEIQRAFADERLTSRMLVDFYLKQIE 65
           L + +++S  + + + + SQI     F+ +EATIE+I+ AF ++RLTS+ LV+ YL+ I 
Sbjct: 12  LLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAIS 71

Query: 66  ALNPVLRSVVEVNPEARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDRMNTT 125
            LNP+L +V+E NP+A  +A+ ADR R   N  +L +  L GVPVL+KD+I+TKD++NTT
Sbjct: 72  KLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPI--LHGVPVLLKDSISTKDKLNTT 131

Query: 126 AGSYALVGSVVARDAGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVNP 185
           AGS+AL+GSVVARDAGVV++LR++GAVILGKASL+EW  FRS   +P+GW A        
Sbjct: 132 AGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFS-IPDGWSA-------- 191

Query: 186 YLASGETCGSSSGSAISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGVI 245
                                                PS +NSVVG KP+VGLT+RAGV+
Sbjct: 192 -------------------------------------PS-QNSVVGIKPSVGLTSRAGVV 251

Query: 246 PIMSRHDTVGPITRTVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPNGSKG 305
           PI  R D++GPI RTVSDAV++LDAIVGYDP D EAT+  S+FIP+GGYKQFL  +G KG
Sbjct: 252 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLD-EATKTASEFIPEGGYKQFLTTSGLKG 311

Query: 306 KRVGVVRTPFADKFPSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELTVMLA 365
           KR+G+V            + ++H+ TLR +G +++++L I +I++I+    SGE   +LA
Sbjct: 312 KRLGIV-------MKHSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLA 371

Query: 366 DFKLLLNDYLKELISSPVRSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGEKEKK 425
           +FK+ LN YLKEL+ SPVRSLADVIA+N    + EK+KE+GQ  F+ +E T+G+GEKEK 
Sbjct: 372 EFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKT 431

Query: 426 AIETMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDKDDGMPFGI 485
           A++ M  LSRNG EKL++E +LDAIVT GS   S+LAIGGYPGI VPAGYD   G+P+GI
Sbjct: 432 ALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYD-SGGVPYGI 462

Query: 486 CFGGLKGTEPKLIEIAYAFEQATMMRRPP 514
            FGGL+ +EPKLIEIA+AFEQAT++R+PP
Sbjct: 492 SFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of Chy11G188660 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 115.9 bits (289), Expect = 9.7e-26
Identity = 136/508 (26.77%), Postives = 226/508 (44.49%), Query Frame = 0

Query: 35  EATIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPEARDEADKADRRRREG 94
           ++ I   +R+      T+  +   YL +I    P L+  + V+     +A + D+R  +G
Sbjct: 43  QSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEIDQRIAKG 102

Query: 95  NVKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILG 154
                 LG L GV + VKD I T+  M +TA S  L       DA  V+K+++ G +++G
Sbjct: 103 E----ELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVG 162

Query: 155 KASLTEWYSFRSLGHVPNGWCARAGQ-GVNPYLASGETCGSSSGSAISVAANMVTVSLGT 214
           K ++ E+     +G       A A Q   NP+  S    GSS GSA +VAA    VSLG+
Sbjct: 163 KTNMDEF----GMGSTTE---ASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGS 222

Query: 215 ETHGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGY 274
           +T GS+  P+    VVG KPT G  +R G++   S  D +G    TV+DA  +L AI GY
Sbjct: 223 DTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGY 282

Query: 275 DPRDAEATRQGSKFIPQGGYKQFLNPNGSKGK-----RVGVVRTPFADKFPS-----MQV 334
           D  D+ +++Q    +P+    QFL+ +  + K     +VG++R    D   S      Q 
Sbjct: 283 DRFDSTSSKQD---VPE-FQSQFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQE 342

Query: 335 FENHLHTLREKGGVIVDDLEIADID-------IILSSKRSGELT----------VMLADF 394
             +HL  L    G I+ ++ +           +I SS+ S  L+          VM  + 
Sbjct: 343 AASHLEAL----GCILTEVSLPSFSLGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEEL 402

Query: 395 KLLLNDYLKELISSPV--RSLADVIAFNNNHPQLEKIKEYGQSTFIQSEKTNGLGEKE-K 454
             L      E     V  R L    A +           Y  + + ++++   L  K+ K
Sbjct: 403 NKLYECSRGEGFGGEVKMRILMGTYALSAG---------YYDAYYKRAQQVRTLIRKDFK 462

Query: 455 KAIE----TMANLSRNGFEKLMKEKELDAIVTPGSGCISMLAIGGYPGITVPAGYDK--D 506
            A+E     ++  + +   K+ ++K+    +  G      + + G P + +P G  +   
Sbjct: 463 AALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGP 521

BLAST of Chy11G188660 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 107.1 bits (266), Expect = 4.5e-23
Identity = 81/242 (33.47%), Postives = 125/242 (51.65%), Query Frame = 0

Query: 37  TIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPE-ARDEADKADRRRREGN 96
           ++ E+       ++TS+ LV  YLKQ++  N VL +VV    E A  +A +AD    +G 
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT 263

Query: 97  VKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGK 156
                LG L G+P  +KD +A      TT GS +     +  +A V ++L+ +GAV++ K
Sbjct: 264 Y----LGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAK 323

Query: 157 ASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGSAISVAANMVTVSLGTET 216
             +T   ++  +      W    G+  NP+     + GSS+G A S +A MV  ++G+ET
Sbjct: 324 L-VTGSMAYDDI------WF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSET 383

Query: 217 HGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDP 276
            GS+  P+ R  +   +PT G   R GV+ I    D +GP  RT +D   +LDAI G DP
Sbjct: 384 AGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDP 431

Query: 277 RD 278
            D
Sbjct: 444 DD 431

BLAST of Chy11G188660 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 101.3 bits (251), Expect = 2.5e-21
Identity = 122/489 (24.95%), Postives = 204/489 (41.72%), Query Frame = 0

Query: 79  EARDEADKADRRRREGNVKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARD 138
           E   +A+ + RR  +GN     +  L+G+ V +KD I           ++      V +D
Sbjct: 176 EVIKQAEASTRRFEQGN----PISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKD 235

Query: 139 AGVVEKLRKAGAVILGKASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGS 198
           + VV KLR  GA++LGKA++ E      LG    G  +  G   NP+     T GSSSGS
Sbjct: 236 SAVVSKLRSCGAILLGKANMHE------LGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGS 295

Query: 199 AISVAANMVTVSLGTETHGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITR 258
           A  VAA + + +LGT+  GS+  PS    + G K T G T   G +      + +GP+  
Sbjct: 296 AAIVAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLAS 355

Query: 259 TVSDAVYVLDAIVGYDPRDAEATRQGSKFIPQGGYKQFLNPNGSKGKRVGVVRTPFADKF 318
           ++ DA  V  AI+G    D    +      P     + L+ NGS    +G +R     K+
Sbjct: 356 SLEDAFLVYAAILGSSSADRYNLKPSPPCFP-----KLLSHNGSNA--IGSLRLGKYTKW 415

Query: 319 PSMQVFENHLHTLREKGGVIVDDLEIADIDIILSSKRSGELTVMLADFKLLLNDYLKELI 378
                                +D+  +DI     S +  ++       KLL N++  +++
Sbjct: 416 --------------------FNDVSSSDI-----SDKCEDI------LKLLSNNHGCKVV 475

Query: 379 SSPVRSLADVIA---FNNNHPQLEKIKEYGQSTFIQSEKTNGLGEKEKKAIETMANLSRN 438
              V  L ++ A    +   P L  +  Y      ++ K + L    + +     + S +
Sbjct: 476 EIVVPELEEMRAAHVISIGSPTLSSLTPY-----CEAGKNSKLSYDTRTSFAIFRSFSAS 535

Query: 439 GF-------EKLMKE-----KELDAIVTPGSGCIS------------------------M 498
            +        +LM+      K++D IVTP +G  +                        +
Sbjct: 536 DYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTTDLMRFV 595

Query: 499 LAIG--GYPGITVPAGYDKDDGMPFGICFGGLKGTEPKLIEIAYAFEQATMMRRPPFSNS 527
           LA    G+P I+VP GYDK +G+P G+   G    E  ++ +A A E+   + + P   +
Sbjct: 596 LAANLLGFPAISVPVGYDK-EGLPIGLQIMGRPWAEATVLGLAAAVEELAPVTKKP---A 607

BLAST of Chy11G188660 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 96.7 bits (239), Expect = 6.1e-20
Identity = 79/242 (32.64%), Postives = 121/242 (50.00%), Query Frame = 0

Query: 37  TIEEIQRAFADERLTSRMLVDFYLKQIEALNPVLRSVVEVNPE-ARDEADKADRRRREGN 96
           ++ E+       ++TS+ LV  YLKQ++  N VL +VV    E A  +A +AD    +G 
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT 263

Query: 97  VKRLSLGGLEGVPVLVKDTIATKDRMNTTAGSYALVGSVVARDAGVVEKLRKAGAVILGK 156
                LG L G+P  +KD +A      TT GS +     +  +A V ++L+ +GAV++ K
Sbjct: 264 Y----LGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAK 323

Query: 157 ASLTEWYSFRSLGHVPNGWCARAGQGVNPYLASGETCGSSSGSAISVAANMVTVSLGTET 216
             +T   ++  +      W    G+  NP+     + GSS+G A S +A       G+ET
Sbjct: 324 L-VTGSMAYDDI------WF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSET 383

Query: 217 HGSILCPSDRNSVVGFKPTVGLTTRAGVIPIMSRHDTVGPITRTVSDAVYVLDAIVGYDP 276
            GS+  P+ R  +   +PT G   R GV+ I    D +GP  RT +D   +LDAI G DP
Sbjct: 384 AGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDP 424

Query: 277 RD 278
            D
Sbjct: 444 DD 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7601.0e-15757.56Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY44.6e-7336.17Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C82.0e-6033.82Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0K3S31.1e-3727.52Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
B0KBN42.4e-3727.33Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
Match NameE-valueIdentityDescription
A0A0A0LPL72.0e-28996.96Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264010 PE=... [more]
A0A5D3BIC81.2e-28194.87Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001... [more]
A0A1S3C5Z21.2e-28194.87putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497305 PE=4 SV=1[more]
A0A6J1CX642.1e-23883.17putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015398 PE=4 SV=... [more]
A0A7N2N0332.0e-21272.83Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147067.20.096.96probable amidase At4g34880 [Cucumis sativus] >KGN61906.1 hypothetical protein Cs... [more]
XP_008457657.10.094.87PREDICTED: putative amidase C869.01 [Cucumis melo] >KAA0045710.1 putative amidas... [more]
XP_038902577.10.088.95LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida][more]
XP_022146104.17.26e-29983.17putative amidase C869.01 [Momordica charantia][more]
XP_030944982.14.23e-26472.83probable amidase At4g34880 [Quercus lobata][more]
Match NameE-valueIdentityDescription
AT4G34880.13.9e-13651.67Amidase family protein [more]
AT3G25660.19.7e-2626.77Amidase family protein [more]
AT5G07360.14.5e-2333.47Amidase family protein [more]
AT5G64440.12.5e-2124.95fatty acid amide hydrolase [more]
AT5G07360.26.1e-2032.64Amidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 55..454
e-value: 5.7E-69
score: 233.3
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 17..514
e-value: 7.2E-143
score: 479.2
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 33..513
NoneNo IPR availablePANTHERPTHR42678:SF25C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 13..520
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 13..520

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy11G188660.1Chy11G188660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane