Homology
BLAST of CcUC03G053180 vs. NCBI nr
Match:
XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])
HSP 1 Score: 914.4 bits (2362), Expect = 4.1e-262
Identity = 440/483 (91.10%), Postives = 457/483 (94.62%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
MEHFLISLLI FLFFLSLT FILFH HKSLFSYPNVPPGAIG PILGESLEFLSTGWKG
Sbjct: 1 MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRL+KYKSDVFKTSIVGVPAAIFCG ACNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
STQT+SKEEAKKLKKFLPQFLKPEALQRYIG+MDELAQRHFASFWENREEVLVFPLAK F
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLFVSV+DEI V+RLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKELVA
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IV++R+W LAEGKAS TQDILSHMLLTCDENG +MNESDIADKILGLLIGGHDT SVACT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+KFLAELPH+Y GVY EQMEIAK KAPGEALKWEDI KMKYSWNV CEVLR+ASP QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREAL DFVFNGFYIPKGWKLYWSAN THK+PEYFPEPYKFEPARFEGNGPLPYT+VPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDP+PIPQKGLP+RLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPHQP 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. NCBI nr
Match:
XP_008443638.1 (PREDICTED: beta-amyrin 28-oxidase [Cucumis melo])
HSP 1 Score: 896.7 bits (2316), Expect = 8.9e-257
Identity = 431/483 (89.23%), Postives = 450/483 (93.17%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
MEHFLISLLIL FFLSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
+TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREALNDFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKFEP RFEGNGPLPYT+VPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. NCBI nr
Match:
XP_004139087.1 (beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein Csa_007564 [Cucumis sativus])
HSP 1 Score: 888.3 bits (2294), Expect = 3.2e-254
Identity = 427/483 (88.41%), Postives = 449/483 (92.96%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
ME FLISLLIL FFLSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
+TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFS+ACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREAL+DFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKF+P RFEGNGPLPYT+VPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. NCBI nr
Match:
XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 857.4 bits (2214), Expect = 6.0e-245
Identity = 410/483 (84.89%), Postives = 442/483 (91.51%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
M FLISLLILFL F+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MADFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+K+LAELP +YD VY EQMEIAKAK GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFRE +NDFVFNGFYIPKGWKLYWS+N THKN YFPEPYKF+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KL++KE IIVDPLPIP+KGLPVRLFP +
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHKY 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. NCBI nr
Match:
KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 856.3 bits (2211), Expect = 1.3e-244
Identity = 410/483 (84.89%), Postives = 442/483 (91.51%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
M FLISLLILFL +SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+K+LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNV CEVLR+ASP+QG
Sbjct: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREA+N FVFNGFYIPKGWK+YWS+N THKN YFPEPYKF+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLL+KE IIVDPLPIP+KGLPVRLFP +
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match:
F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)
HSP 1 Score: 746.5 bits (1926), Expect = 2.0e-214
Identity = 345/480 (71.88%), Postives = 415/480 (86.46%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
ME F +SLL++F+ +S+ +LF+KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1 MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
STQT+SKEEAKK++K LPQF KPEALQRYIGIMD +AQRHFA W+NR+EV+VFPLAKRF
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TF LACRLF+S++D V + +PF + +G+I++PIDLPGTPF+RAIKASNFIRKEL A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
I++QRK LAEGKAS QDILSHMLL DE+G +MNE +IADKILGLLIGGHDT S A T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
F++K++AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREA+ DFVFNGF IPKGWKLYWSAN THK+PE FP+P F+P RFEG+GP PYT+VPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYA+LEILVFMHN+VKRFKW KLL E IIVDP+P+P KGLPVRL PR+P
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRKP 480
BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match:
A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)
HSP 1 Score: 736.5 bits (1900), Expect = 2.0e-211
Identity = 342/480 (71.25%), Postives = 411/480 (85.62%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
ME F +SLL++ + +S+ ++L +KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1 MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
STQT+SKEEAKK++K LPQFLKPEALQRY GIMD +AQRHFA W+NR+EV+VFPLAKRF
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TF LACRLF+S++D V + +PF + +G+I++PIDLPGTPF+RAIKASNFIRKEL A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
I++QRK LAE KAS TQDILSHMLL DE+G +MNE IADKILGLLIGGHDT S A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
F++K++AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREA+ DFVFNGF IPKGWKLYWSAN THK+ E FP+P KF+P RFEG GP PYT+VPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYA+LEIL+FMHNLVKRFKW KLL E IIVDP+P+P KGLPVRL P +P
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHKP 480
BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match:
Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)
HSP 1 Score: 725.3 bits (1871), Expect = 4.7e-208
Identity = 340/478 (71.13%), Postives = 402/478 (84.10%), Query Frame = 0
Query: 24 HFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPE 83
+F +SLL+LF+ F+SL+ F +F+K KS N+PPG +G+PI+GESLEFLSTGWKGHPE
Sbjct: 4 NFYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPE 63
Query: 84 KFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP-SS 143
KFIFDR+ KY S++FKTSIVG + CG A NKFLFSNENKLVT+WWPDSVNKIFP +S
Sbjct: 64 KFIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTS 123
Query: 144 TQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRFT 203
+N KEE+ K++K LPQF KPEALQRY+G+MD +AQRHF + W+N+ E+ V+PLAKR+T
Sbjct: 124 LDSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYT 183
Query: 204 FSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAI 263
F LACRLF+SV+DE V + S PF+ I AGIISLPIDLPGTPFN+AIKASNFIRKEL+ I
Sbjct: 184 FLLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKI 243
Query: 264 VRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTF 323
++QR+ LAEG ASPTQDILSHMLLT DENG MNE +IADKILGLLIGGHDT SVACTF
Sbjct: 244 IKQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTF 303
Query: 324 ILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGA 383
++K+L ELPH+YD VY EQMEIAK+K GE L W+D+ KMKYSWNVACEV+R++ P+QG
Sbjct: 304 LVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGG 363
Query: 384 FREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPFG 443
FREA+ DF+FNGF IPKGWKLYWSAN THKN E FP P KF+P RFEGNGP PYT+VPFG
Sbjct: 364 FREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFG 423
Query: 444 GGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQ 501
GGPRMCPGKEYA+LEILVFMHNLVKRFKW K++ E IIVDP PIP K LP+RL+P +
Sbjct: 424 GGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478
BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match:
A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)
HSP 1 Score: 723.8 bits (1867), Expect = 1.4e-207
Identity = 340/480 (70.83%), Postives = 404/480 (84.17%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKS-LFSYPNVPPGAIGFPILGESLEFLSTGWKG 81
M+ F + LL LF+ F+SL+F+ LF+K KS S +PPG G+P++GES EFLSTGWKG
Sbjct: 1 MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60
Query: 82 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 141
+PEKFIFDRL KY S+VFKTS+ G PAA FCG ACNKFLFSNENKLV +WWPDSVNK+FP
Sbjct: 61 YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120
Query: 142 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKR 201
SSTQT+SKEEA K++K LP FLKPEALQRY+G+MD++A +HF S WEN++EVLVFPLAK
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180
Query: 202 FTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 261
+TF +AC++FVSV++ QV +L PF I +GIIS+PIDLPGTPFN AIK+S +R +L+
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240
Query: 262 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 321
I++QRK L EGKASPTQDILSHMLLT DENG +M E DIADKILGLLIGGHDT S AC
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300
Query: 322 TFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQ 381
TF++KFLAELP +Y+GVY EQMEIAK+K GE L WEDI KMKYSWNVACEVLR+A P+Q
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360
Query: 382 GAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVP 441
GAFREAL DF +NGF IPKGWKLYWSAN TH+N E FPEP KF+P+RFEG GP PY++VP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420
Query: 442 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQ 501
FGGGPRMCPGKEYA+LEILVFMH++VKRFKW K++ E I+V+P+PIP KGLPVRLFP +
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480
BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match:
K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)
HSP 1 Score: 715.7 bits (1846), Expect = 3.7e-205
Identity = 340/478 (71.13%), Postives = 404/478 (84.52%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFH-KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKG 81
ME F SL+ LF+ LSL+F LF+ K+KS+ +PPG G+P++GE+LEFLSTGWKG
Sbjct: 1 MELFYASLVCLFVLLLSLSFLFLFNKKNKSMILSCPLPPGNSGWPVIGETLEFLSTGWKG 60
Query: 82 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 141
HPEKFIFDR++KYKS +FKT ++G A +FCG + NKFLFSNENKLV +WWP SV+K+FP
Sbjct: 61 HPEKFIFDRISKYKSSIFKTHLLGEKAVVFCGASSNKFLFSNENKLVQTWWPSSVDKVFP 120
Query: 142 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKR 201
SSTQT+SKEEA K++K LP FLKPEALQRYI IMD +AQ+HF S WEN++EV+VFPLAKR
Sbjct: 121 SSTQTSSKEEAIKMRKMLPNFLKPEALQRYIRIMDHIAQKHFQS-WENQQEVVVFPLAKR 180
Query: 202 FTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 261
+TF LACRLFVSV+D V RL+ PF+ + +G+IS+PI+LPGTPFNRAIKASNFIRKELV
Sbjct: 181 YTFWLACRLFVSVEDPNHVARLADPFDVLASGLISIPINLPGTPFNRAIKASNFIRKELV 240
Query: 262 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 321
AI++QRK L EGKAS +QDILSHMLLT DENG +M+E DIADKILGLLIGGHDT S AC
Sbjct: 241 AIIKQRKIDLTEGKASDSQDILSHMLLTSDENGKFMHELDIADKILGLLIGGHDTASSAC 300
Query: 322 TFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQ 381
FI+K+LAELP +YD VY EQ+EIAK+K PGE L WEDI KMKYSWNVACEVLR+A P+Q
Sbjct: 301 AFIVKYLAELPEIYDQVYKEQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQ 360
Query: 382 GAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVP 441
GAFREAL DF FNGF IPKGWK+YWSAN TH NPE F +P KF+P+RFEGNGP PYT+VP
Sbjct: 361 GAFREALVDFTFNGFSIPKGWKIYWSANSTHINPEVFVDPLKFDPSRFEGNGPAPYTFVP 420
Query: 442 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFP 499
FGGGPRMCPGKEYA+LEILVFMH+LVKRF W K++ E I+V+P+PIP GLP+RL+P
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHLVKRFSWKKIIRDEKIVVNPMPIPANGLPIRLYP 477
BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match:
A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)
HSP 1 Score: 896.7 bits (2316), Expect = 4.3e-257
Identity = 431/483 (89.23%), Postives = 450/483 (93.17%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
MEHFLISLLIL FFLSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
+TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREALNDFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKFEP RFEGNGPLPYT+VPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match:
A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)
HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 427/483 (88.41%), Postives = 449/483 (92.96%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
ME FLISLLIL FFLSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
+TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFS+ACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREAL+DFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKF+P RFEGNGPLPYT+VPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match:
A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)
HSP 1 Score: 855.9 bits (2210), Expect = 8.5e-245
Identity = 410/483 (84.89%), Postives = 441/483 (91.30%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
M FLISLLILFL +SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+ +LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREA+N FVFNGFYIPKGWK+YWS+N THKN YFPEPYKF+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLL+KE IIVDPLPIP+KGLPVRLFP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480
Query: 502 LSL 505
LSL
Sbjct: 481 LSL 483
BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match:
A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)
HSP 1 Score: 851.3 bits (2198), Expect = 2.1e-243
Identity = 411/485 (84.74%), Postives = 441/485 (90.93%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
ME FLISLLILFL F+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLF+S++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IVRQRK LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDT S ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEI--AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 381
FI+K+LAELP +YD VY EQMEI AKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360
Query: 382 QGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYV 441
QGAFREA+NDFVFNGF IPKGWKLYWS+N THKN YFPEPYKF+P+RFEGNGP PYT+V
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
Query: 442 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPR 501
PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLEKE IIVDPLPIP+KGLPVRLFP
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480
Query: 502 QPLSL 505
+ LSL
Sbjct: 481 KTLSL 485
BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match:
A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)
HSP 1 Score: 849.4 bits (2193), Expect = 7.9e-243
Identity = 399/482 (82.78%), Postives = 437/482 (90.66%), Query Frame = 0
Query: 22 MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
MEHFL+SLLI F+ + + FILF+KHKS FSYPN+PPG IGFPI+GESLEF STGWKG
Sbjct: 1 MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
Query: 82 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
STQT+S EEAKKLKK LPQFLKPEAL RYIGIMD LA RHFAS WEN+++VLVFPLAK F
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
TFSLACRLFVSV+DE VERLS PF+ I AGIIS+PID PGTPFNR IKASNFIRKEL++
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
IV+QRK LAEGKASPTQDILSHMLLTCDENG YMNE DIADKILGLLIGGHDT S ACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
FI+KFLAELPH+YDGVY EQM IAKAKAPGE L WEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
AFREA+NDFVFNGFYIPKGWKLYWS+N THKN +YFP+PY F+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
GGGPRMCPGKEYA+LEILVFMHNLVKRFKWAK+LEKE I+VDP+PIPQKGLPVRLFP +
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
Query: 502 LS 504
+S
Sbjct: 481 VS 482
BLAST of CcUC03G053180 vs. TAIR 10
Match:
AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )
HSP 1 Score: 638.6 bits (1646), Expect = 4.1e-183
Identity = 301/479 (62.84%), Postives = 367/479 (76.62%), Query Frame = 0
Query: 25 FLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEK 84
++ ++ILFL + L+ +L KH S FSYPN+PPG G P++GES FLS G +GHPEK
Sbjct: 2 YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61
Query: 85 FIFDRLNKYKSD---VFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 144
FI DR+ ++ S VFKT + G P A+ G + NKFLF+NENKLV SWWPDSVNKIFPS
Sbjct: 62 FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121
Query: 145 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 204
S QT+SKEEA+KL+ L QF+KPEAL+RY+G+MDE+AQRHF + W N+++V+VFPL K+F
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 181
Query: 205 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 264
TFS+ACR F+S++D +V +L F + GI S+PIDLPGT FNRAIKAS +RKE+ A
Sbjct: 182 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 241
Query: 265 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 324
IVRQRK L GKA DILSHML+ E + D+ADKI+GLLIGGHDT S+ CT
Sbjct: 242 IVRQRKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCT 301
Query: 325 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 384
F++ +LAE PHVY V EQ EI K K E L+WEDI KM+YSWNVACEV+RI P+ G
Sbjct: 302 FVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSG 361
Query: 385 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 444
FREA++ F F GFYIPKGWKLYWSA TH NP+YFPEP +FEP RFEG+GP PYTYVPF
Sbjct: 362 TFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPF 421
Query: 445 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKEN-IIVDPLPIPQKGLPVRLFPR 500
GGGPRMCPGKEYA+LEIL+FMHNLV RFKW K+ EN I+VDPLPIP KGLP+R+FP+
Sbjct: 422 GGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 476
BLAST of CcUC03G053180 vs. TAIR 10
Match:
AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )
HSP 1 Score: 377.1 bits (967), Expect = 2.2e-104
Identity = 184/318 (57.86%), Postives = 237/318 (74.53%), Query Frame = 0
Query: 29 LLILFLFFLSLTFFILFH--KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 88
L I+FLF S+ F +LF KH S F YPN+PPG IGFP++GE+L FLS G +GHPEKF+
Sbjct: 3 LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62
Query: 89 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPSSTQTN 148
DR+ + S +FKT + G P A+ G + NKFLF+NENKLV SWWPDSVNKIFPSSTQT+
Sbjct: 63 TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122
Query: 149 SKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRFTFSLA 208
SKEEA K + L +KPEAL+RY+G+MDE+AQ+HF + W N+++++VFPL K+FTFS+A
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182
Query: 209 CRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 268
CRLF+S+DD +V +L PF ++ G+ S+PIDLPGT FNRAIKAS + KE+ I+RQR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242
Query: 269 KWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 328
K L GK S QDILSHML+ E + D+ADKI+ LLIGGHDTTS+ CTF++ +
Sbjct: 243 KEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNY 302
Query: 329 LAELPHVYDGVYAEQMEI 345
LAE PH+Y V E M+I
Sbjct: 303 LAEFPHIYQRV-LEGMQI 315
BLAST of CcUC03G053180 vs. TAIR 10
Match:
AT2G42850.1 (cytochrome P450, family 718 )
HSP 1 Score: 279.3 bits (713), Expect = 6.3e-75
Identity = 165/486 (33.95%), Postives = 261/486 (53.70%), Query Frame = 0
Query: 24 HFLISLLILFL-FFLSLTFFILFHKH-----KSLFSYPNVPPGAIGFPILGESLEFLSTG 83
+F++S + L + +S T F KH K + + PG +G P +GE+++F
Sbjct: 6 NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65
Query: 84 WKGHP-EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWP-DSV 143
E F+ R+ K+ ++FKT I+G P + G N+ + SNE LV S WP SV
Sbjct: 66 KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125
Query: 144 NKIFPSSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVF 203
+ + E+ + L+ + L L+ I + + + H + W +EE+ ++
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185
Query: 204 PLAKRFTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFI 263
AK TF++ + EI + + FE+++ G+ +LP++ P + F RA KA I
Sbjct: 186 RSAKVLTFTVVFECLYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEI 245
Query: 264 RKELVAIVRQ-RKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHD 323
LV VR+ R+ + EG P + S ++ + + E ++ D ++ L+ HD
Sbjct: 246 ETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELIKG--VITEEEVVDNMVLLVFAAHD 305
Query: 324 TTSVACTFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLR 383
TTS A + K LA+ P D + E +I K GE L ED+ KMKYSW V E +R
Sbjct: 306 TTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMR 365
Query: 384 IASPVQGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPL 443
++ P+ G+FR+A+ D + G+ IPKGWK+ W+ GTH NPE F +P F+P RF+ P+
Sbjct: 366 LSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFD--KPI 425
Query: 444 -PYTYVPFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLP 500
YTY+PFGGGPR+C G + AK+ ILVFMH +V F W+ + E I +DPLP P G+P
Sbjct: 426 QAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMP 483
BLAST of CcUC03G053180 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 254.6 bits (649), Expect = 1.7e-67
Identity = 153/479 (31.94%), Postives = 254/479 (53.03%), Query Frame = 0
Query: 23 EHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHP 82
E LI+L I+ + + L + L K + L +PPG++G P +GE+L + +P
Sbjct: 19 EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENP 78
Query: 83 EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNK-IFPS 142
F R NKY D+FKT I+G P + P + + ++ L +P S + I P
Sbjct: 79 NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138
Query: 143 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 202
+ + LK+ + P AL+ + ++ L + +S W +++ + KR+
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSS-WTSQKSINTLEYMKRY 198
Query: 203 TFSLA-CRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 262
F +A F ++ ++ + ++++ G S+P+DLPGT F++++KA + +EL
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258
Query: 263 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 322
++ +R+ +L +L D+ +++S IAD I+G++ DTT+
Sbjct: 259 KVIEKRR-----ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318
Query: 323 TFILKFLAELPHVYDGVYAEQMEI-AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 382
T++LK+L + P++ V EQ I K K + WED KM + V E LR AS +
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378
Query: 383 QGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYV 442
FREA+ D ++G+ IPKGWK+ H + E+FP+P KF+P+RFE P PYTY+
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438
Query: 443 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFP 499
PFG G CPG E AKLE+L+ +H+L F+W + ++E I P P+P+KGLP+R+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
BLAST of CcUC03G053180 vs. TAIR 10
Match:
AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 254.6 bits (649), Expect = 1.7e-67
Identity = 153/479 (31.94%), Postives = 254/479 (53.03%), Query Frame = 0
Query: 23 EHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHP 82
E LI+L I+ + + L + L K + L +PPG++G P +GE+L + +P
Sbjct: 19 EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENP 78
Query: 83 EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNK-IFPS 142
F R NKY D+FKT I+G P + P + + ++ L +P S + I P
Sbjct: 79 NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138
Query: 143 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 202
+ + LK+ + P AL+ + ++ L + +S W +++ + KR+
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSS-WTSQKSINTLEYMKRY 198
Query: 203 TFSLA-CRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 262
F +A F ++ ++ + ++++ G S+P+DLPGT F++++KA + +EL
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258
Query: 263 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 322
++ +R+ +L +L D+ +++S IAD I+G++ DTT+
Sbjct: 259 KVIEKRR-----ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318
Query: 323 TFILKFLAELPHVYDGVYAEQMEI-AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 382
T++LK+L + P++ V EQ I K K + WED KM + V E LR AS +
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378
Query: 383 QGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYV 442
FREA+ D ++G+ IPKGWK+ H + E+FP+P KF+P+RFE P PYTY+
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438
Query: 443 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFP 499
PFG G CPG E AKLE+L+ +H+L F+W + ++E I P P+P+KGLP+R+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038877759.1 | 4.1e-262 | 91.10 | LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida] | [more] |
XP_008443638.1 | 8.9e-257 | 89.23 | PREDICTED: beta-amyrin 28-oxidase [Cucumis melo] | [more] |
XP_004139087.1 | 3.2e-254 | 88.41 | beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein ... | [more] |
XP_023516848.1 | 6.0e-245 | 84.89 | beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo] | [more] |
KAG6590124.1 | 1.3e-244 | 84.89 | Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >K... | [more] |
Match Name | E-value | Identity | Description | |
F6H9N6 | 2.0e-214 | 71.88 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1 | [more] |
A5BFI4 | 2.0e-211 | 71.25 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2 | [more] |
Q2MJ20 | 4.7e-208 | 71.13 | Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... | [more] |
A0A0S2II38 | 1.4e-207 | 70.83 | Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... | [more] |
K4CEE8 | 3.7e-205 | 71.13 | Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3B8H4 | 4.3e-257 | 89.23 | beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1 | [more] |
A0A0A0LXN3 | 1.5e-254 | 88.41 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1 | [more] |
A0A6J1H8T4 | 8.5e-245 | 84.89 | beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1 | [more] |
A0A6J1JJQ7 | 2.1e-243 | 84.74 | beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1 | [more] |
A0A6J1DZX7 | 7.9e-243 | 82.78 | beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT5G36110.1 | 4.1e-183 | 62.84 | cytochrome P450, family 716, subfamily A, polypeptide 1 | [more] |
AT5G36140.1 | 2.2e-104 | 57.86 | cytochrome P450, family 716, subfamily A, polypeptide 2 | [more] |
AT2G42850.1 | 6.3e-75 | 33.95 | cytochrome P450, family 718 | [more] |
AT2G29090.1 | 1.7e-67 | 31.94 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |
AT2G29090.2 | 1.7e-67 | 31.94 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |