CcUC03G053180 (gene) Watermelon (PI 537277) v1

Overview
NameCcUC03G053180
Typegene
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
Descriptionbeta-amyrin 28-oxidase
LocationCicolChr03: 21440000 .. 21448732 (-)
RNA-Seq ExpressionCcUC03G053180
SyntenyCcUC03G053180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTGCCTTTATATTTCCCACAAAAATGACCAATCTCCCTCCAACCTTCTTCCTCTCTCCAGTTCAAAACCTCTTTCTCAACGTAACAATGGAGCATTTCTTAATCTCTCTCTTAATCCTCTTCCTCTTTTTCCTCTCTCTCACTTTCTTCATCCTCTTCCACAAGCACAAATCCTTGTTCTCCTATCCCAACGTTCCTCCCGGCGCCATAGGCTTTCCCATTCTCGGTGAGAGCCTCGAGTTCTTGTCCACTGGCTGGAAAGGCCACCCTGAGAAGTTCATCTTCGACCGTTTGAATAAGTACAAGTCTGATGTCTTCAAAACCTCCATCGTCGGCGTTCCGGCCGCCATTTTCTGCGGCCCTGCGTGCAACAAGTTCCTCTTCTCTAACGAGAATAAACTCGTTACTTCTTGGTGGCCGGATTCCGTTAACAAGATCTTTCCCTCTTCCACTCAGACTAACTCCAAAGAGGAAGCTAAGAAACTCAAGAAATTCCTCCCGCAATTCCTTAAGCCGGAAGCGCTTCAGCGTTATATTGGAATTATGGATGAACTCGCTCAACGCCATTTCGCTTCCTTCTGGGAGAACAGGGAAGAGGTCCTTGTGTTTCCTCTCGCTAAAAGGTAAAATATCAAACAGTTATAGTTTAATCGAAATTCAATTTCTCCAATCTTTGATTTCGAATTTGATTGATCGACTTCCGATAATGGATTAGCTTCATGTTCTCGTTAGATTTCGATTTCTTGTGTGAATTCTGATTTCGACTTCCGATAATGGAATTAACCTTTCTCTTTCGATTTAGATTTCGATTTCGTCTTTTGTTGCGAATTCTGATTGATCGACTTCCGAGAATGTGATTAGCTTCTTCACGTTCCCGTTCGATTTCTGCAGTGAACTCTGATTGATCGATTTCCGATGGAACTAGCTTCTTCACAATCTCGTTGTCCGTTCTAATTTTTCTTAAACTGATTTCCGATGAAATTAGGTTCACATTCTCGCTGGCGTGCCGATTGTTCGTGAGCGTAGACGATGAAATCCAGGTGGAGAGATTATCGAGACCGTTTGAGCAAATTGTGGCAGGAATCATATCGTTGCCGATCGATTTACCAGGAACGCCGTTCAATCGAGCAATAAAGGCATCGAATTTCATCAGAAAGGAACTGGTGGCGATCGTGAGGCAGAGGAAATGGGTTCTGGCGGAAGGAAAGGCGTCGCCGACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGATTTTACATGAACGAGTCCGATATCGCCGATAAGATTCTGGGGCTATTGATCGGCGGCCATGACACTACCAGTGTCGCATGCACCTTCATCCTTAAGTTCCTTGCTGAGCTTCCTCATGTGTACGATGGCGTCTACGCGGGTAAGCTTAATATATATGAAAAGACTAATACATCCCTCTCCATATACATCATCCTCAAAATAATATTTTAAAATTTTCTTTCTTTACAAAAACGAAGAAAAGAAAATAAAATAGTAAACTTCAGAAAAACACCCCTAAAAGTTTAGGGGTAGTTATAATTATATTTTCAAATCGAGTCTTAAACAAAGTGTTAAAATTCAACCATCAAACTTAAATAATTGTAAATATTGTAACAATGGTATTGGTTCGAGGAGTTAAATGTATCATTTGAGAGTCCAATTTTTACAACCGTAAATTTTAAAGTCTAATTTTAACATTTAAAGAAGTTTGAAGATCTAATTTATACAATTGAAAGTTTGAAAATGTAATTAAATTACCATTATAATTCAAGGATGATTTTTGCATTTTGCCCAAACAATTACATGCTAAACTTATGATTATGAATTATGTGGACACACCCGGTAGTTGCAATAAAGATGCACCAAATGAAAAGTAATATGGTGTATTCCTATCTACACCTAGGCCACTAATCACAATTTATCATGTGACAAACTCTTTTTCCTTTTTAAAAAATTGATCTATGTGTAATTGATTTGGAAGATGGATGCAAGCTCAGCTAGAGAAGTATTACTTGAGCATTGATTTTACAAAATTTTCAAATCACATTTACTTATTTAGATTAGAAAAAAAGTTTGTTGAATACATAATTATAAGGGTGTTCAAAAGACTAGATGACCCAACTCAACCCCTACAATTTGGTTGGGTTATAAACTCATTTGGGTTGGGTCGGGTTCAAATAAATAAAATTGGTTAAAATACCATTTTAATCTCTATATTTTATAATTTCTTAAATTTTAGTCCATATACTTTTAAATGTCTAATTTTAGTCTTTATACTTTCGATAAATCTTAAATGTAGTCTCTCAAAGTTAAATTTTATTGAAATGGATTAAATAATTACAATATCTTTCATGCAAAGAAAAACAATATGTAAATATGTTCTCAAAACTTATAGTGAAAATGATAATAAAAAAATAAAAGGTAATGAAAAAATTCACAACCAACTAGTAGGAGAGATTAAATTTAAGATTTATTGAAAGTAAAAATACTAAAATTGAACATTTGAAAGTACGCTAAAATTAAACAAACTCCAAAGTATAGAGACCAAAATTATATTTTTCACCATAAAAAATTTTATGGACTGTATTGGTTATGGATTCACTTAAAATAACTCAAACCAACCAAAATCAACCTGAACTTCTGAATAATTATAAAAAGTACGTCTTTTACTTATAATAATATATTTATATATGCATTATATTTATTTTAATTTTATTAATTTTTTGGATCAATAATTCTCCAAAAACTTCTAAAAATAATTGGTAACGACTTTGTAAAGAAAAAAAATTCATAATATAATTTGAAATTGAGTTCTTAATGTTAATCCAATATATGAAAATAATTAAATAAAACTTTTATATATTAACATTTTATTTATTGCTAGACGAAAAACTTAAATAAATGACTTGAGTAGCCTAAACCAACCAAACCCAAATGTACCATCGGGTTAGGTTAGGTTTATTATTAATAAGAGTTTTTTGGGTTGAAAATCCTTACAATCCGAATAATTGAATTGGGTCTATAAGGTACTTCCATCCAACCCAACCCACGAACATCCCTACCTATTTTTGTATAGTTCATTCAATAATTTTATCATAGTTTGTTAAGTGACAATTTTTTTTTAAAAAAACATTATTTTAAAATTTACTTTAATCATAGTTTGCTAACTAAATATTTCTATGTGATGGGAAGAGTTCTCTATGTAGTGCTGTTTAAATTAAGAAAAACAAATTTTTAGTTTGAGTCTAGAGACTACCTCTTGTTCACGTATATAGCTCACTGAAAAAGTTGAACCGTTTTTAAAAGTTGAACAGTTTTTAATCTCTCTACGTCTCAATTATATATATGTATAACATGGTAATGGAAGAAAATTAAAGTGAGGGAAACAAAAATTTTTAGGTTAAAAGACTACTGAATCACTAGTTTTACATTATTTTAGTCATATATTTTTAAAAATTCATGAAAATTTTAAATTAGTCTTTTTAGTTATGTTTGTTGATTTTATTTTTTTAAAAAATAATTACTTTATAAATTATAAATATATATTCTCATTTTGATATTAAAATTATTATTATTATTTATGAATTTTATTAAATTTAAGATGGATGAAATATATAAAAACTAACTTAAAATTGATTGAAAATATTAAAACTATAAAATTTAACCATTAAAAATATAGAGACCAAAATAAAATTGATCTCAGAATAGATGTACCACAAAACTTTTGAGTCTTCTACTTTACGTTTATCAATTTTAGTTATGTACTTTTAAATATTTAATTTTAGTTTTTATATTTTTAATAAATTTTGAAATGGTCGTAATTTTTTTTTTTAAAAAAAGTTTCAATGGCATTTTTCTCTTATAAATTTTAGAAATATATTTGTTCATAGATTAACTTTTCCGACATGAAAATTAGTGTTATTATTATTATTTTACTGATTTGATGAAAGTAAACTTGAAGATAAACATTTTAAGATTATTGAGAATGCACGCACTAAAATCGAACCATTGTAATTACATCAACTAAAAATAAATAAAATTCAAATTCTAAAGCAGATTATGTAACCTAAGAAATTTGTATAATAAAAATAATATTTAAATTTTCTCCTTCACTTTTCTTTTTATATTTTTAATATCATTTATATTTGTGTCGGTGAAATCTTAAAGTTTAATTGACAAGTTTACTCTCGCAATATTTTTAGTTGGCATATATTTAGTAATAAATTTTTAATTTTTTTTAATAAGAATATGAACTTTCAATTTTATGTCCAATAAATCTTTAAATTTAAGGGATGTCCAATAAGTCTCTAATTATTCAATTTCGTGTCCGATAAATTTTTAAACCTTAAAAAATATCTAATTTAATTTTATCTTGAGAGTATATAACCTATTTAAAGATTTTTTATCTCACCTATATATATATAACTATAAAGATTTTACAATTTTGTTTTCCTAATTCGTTCTTTGTTTTTAATGACAAAACTACTGTAAATATTTTCAAGCATAGCACAATGTTATTGATACTAATAGATAGTGACATTTTACTATATTTATAAATATTTTAGCTCATTTTGCTATATTTGAATTAGTTTATTAAGAAATAATAGTAATAAAATTAAGGATGAAAATGATATTACTAATGAACTAATTTTTATAATTTAATTTTTCTTTAATTGTTTTATTTTTAAGTATTGCATTTGGAAAGAAGTGTTTTGTTTCTTAAAAATAGAAAACTTAAAAAAAAAAAAGAAAAAAAGTCATCTAACAAATTGTAGTTAGACAATTAAATAAAAGTGTAGGATACGTCTAAGTATTTTTCTCAGTATAATTTTTTTATATCAAAATAATTCCACGACCAAAATAATAATATTATTATAATTTTTTTGCAAATTGATTAACAAATTAATTTGCAAAATTTGAATATGAACAGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGGGAAGCATTGAAGTGGGAAGACATAAACAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTTCCCCAGTCCAAGGTGCCTTTAGGGAAGCCTTAAACGACTTCGTTTTTAATGGCTTCTACATTCCCAAGGGCTGGAAGGTAATTTTCTCTCCTTCCTCCTTTACCTTTAAATATATATTAAAATTAGTATAAATAAAAGAATATCAAACTATTTACAAATATAAAAAAAATTTACTTTGATACACCGCGATAGACTACATCTATTCGTGTCTATCTAGTAAAAAGATAAATATCTATTTAGATCTATCACACGTTTACTATATTTGTAATTAATTTCAACCGTTTTTTTATTTTTAAAACACATATATTTTATATGTGTGTTTTTTTTTAAAAAAAAAACTGTTGGCATGTTTGAACAATGACCATTAACAATTGACCAAACTAATAAAAATTGGTCATCATTAACTTTACCTATTAACTAACCATAATTTCAAATGTATCACCCATCTTTTTTTATTTTATTTTATTTTATTTTTTATGTGTGTATATATATATATGGTGGTGTCAAGAACACAAATACTTTATATATGACTTCTAAGCTTTAAAGAATGTTTAATAGGTATGAATTTTTTATTTTTATATTTAATAGATTTCTATATATTAATTATGAATAGGTTTCATATTAAAAATGTTAAAAAGCTATATATTATATTAGACATTAAATTTAATATTGTTGTATGATTAGATATCTTTTTAAAATTAATTTTATCTATTAACTACAAAATTTACCACTTAAAGGTTTAATTAGACACAATATATATATTTATATATAACAATCAATGATTAAATATATATATATATATATATATATATATATATATATATATATATATATAACAATCAATTTTTAAATATTTTAATTATTTATAAGATTTATTAAATATAAAAATTAAAGTTTATATATTTATTAGTCATTTTTAAGGATTAGAGTCAAAATTGAAAGTTTGAGAATTTATTAGCAATAATTTTCAAAAATTAGAGACTAAACATGTAATTTAACCTTCATATAAATGTAATGCATTGATATATGATATTTAGATTAAATGATATTAATTACAAAATTATTATAAATCTTTATAAAATTATTGACTTAAATATATGAGTAAAAATCATTCCATCATCTCTCGTAAAATAATTTTATAAAGATCCCAAAAATGTAATTAAGTTTTTTGGACCTATTTGAAGTGTATTAAAAATTAAAATATTATATATATTTTTCAAAAAAATTAAAAATAAATGTAAATATTTTTGTGACTAATTCATTTATATATATTATATAGAAAGATTTGAAATATGAAATTGAAATGTTTTAAAAAAAGAAAAAAAAAAAAAGAAAAAGAAAATTGCCCTTCAAATTTGAAGCTTGGTTTTGAGGCGGCAGAAAACTAATGCACATCACACCACCGCGGGAATAGATAATGTTAGCAGTAAAAATTAAACGAGCATTTTAATGAAATATAACTGTCAATATTTAATAGTTAATGAAAATCAATATATAATTTATGTAAAATGTCACGATGTGGGCACGTGCTCCCGACCCTTCGTACATCCAGTCATATATTTGATTGATTGACATATATATCTCTTGACATATATTTTCATTCTATCTTTTTACTATTATATTTTTATTTCAATTTTATAATCAATAAACATGAGATCTATAGATGTGAGTATGTGACTATAAGTAGATAGATTTAGGACATTGTTTCGATTAATTTAAAAAATAAATATTTTTCATAAATTTATTTTTATTTAAATACTTTTGATAATAATTTTTTAAAATAAAAATAAAAATAAAAATAATTTTTAAATTTTATTTTAGTTGCCAAAAATTTTTATTTCTCTCAAAATGACTTATTTTTTAAATACTTGAAAAGATATTCCAACGCACCCTAATTAAGTGATCAAGCAAAAATGAAAGAAAGATTTTATCTATCATTTTAAGTAAAAAATATTAAAAGTTTTCCTTTTTTCTAAATTGTTCTTCAAAATTGTAAACGTATCATTATTTTTAGAATTATTTTAAATGGCAAAATTGCTAAAAGTATTTTCAAATATAGTAAAATGTCACTGTCTATTATTGATAGATAATGATAATCACTAATAGATGACAATAGTAGACTATCTATTAATGTTTATCATTGATAAATAGTAACATTTTCCTACATTGATTATTTGTAAATATTTTGGTCAATTTTGCTATATTTAGAAACAGGGTTATTTTTTTTTATTTTTTTTATTTTTTTACAGAGTATAAATACTAGTCAGAACCTAATAGTTTGCTATTTTTATTAAAAGAAATATACAAAAACAAATTTAAAATTTTAACTACACAAATGAGAATATAGTTCTTTTCCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTCTTTTAATAATTAAGGAGATGAAGGATGAAATTTTCCATTCTACTAACTTCATCCCTCTCCTGTAATTGTTGAACTAAAAAAATCAATAATAAAATTATAGTGTATCCGTAGAGAAATTATCAAAGAGGAGGTCATCACAATAGAAAAAGACCATAAAAGTATCTCGAATTTGTATCTAGTAGGTTATTAGGAGTTGAGCATGCAAGTTTTAAAGATAGCCATTGTTAGAATGCCTTGAATATGCACAGTCATCGTTACGATTTATATTATCTGATTTTTTCTCCAATAAAATCTTATCTATCCCTCTGTTCGTGGATACGTAGCTAATACACAATTAGTGAACCGCGTAAATCTCTATGTTGATTTACTTTACACATTTTCTTTGGTTTGTCGATTTCATAACTGCCACTGTAACGATATAGTTAAATAAATTTTCGATATCTTAATGACATACTAGATCAAATATTGTTTATTTTCAGCTATATTGGAGTGCAAATGGAACACACAAAAACCCAGAGTACTTCCCAGAGCCGTACAAGTTTGAGCCAGCAAGATTTGAAGGGAATGGGCCATTGCCCTACACTTATGTGCCTTTTGGGGGAGGGCCAAGGATGTGCCCTGGAAAAGAGTATGCAAAGCTTGAGATTTTGGTGTTCATGCATAATTTGGTCAAGAGATTCAAATGGGCAAAGCTACTTGAAAAGGAGAATATCATTGTTGACCCATTGCCAATCCCTCAAAAAGGTCTCCCCGTTCGCCTTTTTCCTCGTCAACCTCTCTCTCTTTAGCCAATTCCCATAATATTATTATATATATGCATAATACTCTTTAATTTCAACTTCCTCTTATCATATATTATCAATAAATCATGATATATATCTATATCTATATATATTATACACTTCTTATAGTTATATAATTCTCTCTTCCACTATA

mRNA sequence

TTGTGCCTTTATATTTCCCACAAAAATGACCAATCTCCCTCCAACCTTCTTCCTCTCTCCAGTTCAAAACCTCTTTCTCAACGTAACAATGGAGCATTTCTTAATCTCTCTCTTAATCCTCTTCCTCTTTTTCCTCTCTCTCACTTTCTTCATCCTCTTCCACAAGCACAAATCCTTGTTCTCCTATCCCAACGTTCCTCCCGGCGCCATAGGCTTTCCCATTCTCGGTGAGAGCCTCGAGTTCTTGTCCACTGGCTGGAAAGGCCACCCTGAGAAGTTCATCTTCGACCGTTTGAATAAGTACAAGTCTGATGTCTTCAAAACCTCCATCGTCGGCGTTCCGGCCGCCATTTTCTGCGGCCCTGCGTGCAACAAGTTCCTCTTCTCTAACGAGAATAAACTCGTTACTTCTTGGTGGCCGGATTCCGTTAACAAGATCTTTCCCTCTTCCACTCAGACTAACTCCAAAGAGGAAGCTAAGAAACTCAAGAAATTCCTCCCGCAATTCCTTAAGCCGGAAGCGCTTCAGCGTTATATTGGAATTATGGATGAACTCGCTCAACGCCATTTCGCTTCCTTCTGGGAGAACAGGGAAGAGGTCCTTGTGTTTCCTCTCGCTAAAAGGTTCACATTCTCGCTGGCGTGCCGATTGTTCGTGAGCGTAGACGATGAAATCCAGGTGGAGAGATTATCGAGACCGTTTGAGCAAATTGTGGCAGGAATCATATCGTTGCCGATCGATTTACCAGGAACGCCGTTCAATCGAGCAATAAAGGCATCGAATTTCATCAGAAAGGAACTGGTGGCGATCGTGAGGCAGAGGAAATGGGTTCTGGCGGAAGGAAAGGCGTCGCCGACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGATTTTACATGAACGAGTCCGATATCGCCGATAAGATTCTGGGGCTATTGATCGGCGGCCATGACACTACCAGTGTCGCATGCACCTTCATCCTTAAGTTCCTTGCTGAGCTTCCTCATGTGTACGATGGCGTCTACGCGGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGGGAAGCATTGAAGTGGGAAGACATAAACAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTTCCCCAGTCCAAGGTGCCTTTAGGGAAGCCTTAAACGACTTCGTTTTTAATGGCTTCTACATTCCCAAGGGCTGGAAGCTATATTGGAGTGCAAATGGAACACACAAAAACCCAGAGTACTTCCCAGAGCCGTACAAGTTTGAGCCAGCAAGATTTGAAGGGAATGGGCCATTGCCCTACACTTATGTGCCTTTTGGGGGAGGGCCAAGGATGTGCCCTGGAAAAGAGTATGCAAAGCTTGAGATTTTGGTGTTCATGCATAATTTGGTCAAGAGATTCAAATGGGCAAAGCTACTTGAAAAGGAGAATATCATTGTTGACCCATTGCCAATCCCTCAAAAAGGTCTCCCCGTTCGCCTTTTTCCTCGTCAACCTCTCTCTCTTTAGCCAATTCCCATAATATTATTATATATATGCATAATACTCTTTAATTTCAACTTCCTCTTATCATATATTATCAATAAATCATGATATATATCTATATCTATATATATTATACACTTCTTATAGTTATATAATTCTCTCTTCCACTATA

Coding sequence (CDS)

ATGACCAATCTCCCTCCAACCTTCTTCCTCTCTCCAGTTCAAAACCTCTTTCTCAACGTAACAATGGAGCATTTCTTAATCTCTCTCTTAATCCTCTTCCTCTTTTTCCTCTCTCTCACTTTCTTCATCCTCTTCCACAAGCACAAATCCTTGTTCTCCTATCCCAACGTTCCTCCCGGCGCCATAGGCTTTCCCATTCTCGGTGAGAGCCTCGAGTTCTTGTCCACTGGCTGGAAAGGCCACCCTGAGAAGTTCATCTTCGACCGTTTGAATAAGTACAAGTCTGATGTCTTCAAAACCTCCATCGTCGGCGTTCCGGCCGCCATTTTCTGCGGCCCTGCGTGCAACAAGTTCCTCTTCTCTAACGAGAATAAACTCGTTACTTCTTGGTGGCCGGATTCCGTTAACAAGATCTTTCCCTCTTCCACTCAGACTAACTCCAAAGAGGAAGCTAAGAAACTCAAGAAATTCCTCCCGCAATTCCTTAAGCCGGAAGCGCTTCAGCGTTATATTGGAATTATGGATGAACTCGCTCAACGCCATTTCGCTTCCTTCTGGGAGAACAGGGAAGAGGTCCTTGTGTTTCCTCTCGCTAAAAGGTTCACATTCTCGCTGGCGTGCCGATTGTTCGTGAGCGTAGACGATGAAATCCAGGTGGAGAGATTATCGAGACCGTTTGAGCAAATTGTGGCAGGAATCATATCGTTGCCGATCGATTTACCAGGAACGCCGTTCAATCGAGCAATAAAGGCATCGAATTTCATCAGAAAGGAACTGGTGGCGATCGTGAGGCAGAGGAAATGGGTTCTGGCGGAAGGAAAGGCGTCGCCGACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGATTTTACATGAACGAGTCCGATATCGCCGATAAGATTCTGGGGCTATTGATCGGCGGCCATGACACTACCAGTGTCGCATGCACCTTCATCCTTAAGTTCCTTGCTGAGCTTCCTCATGTGTACGATGGCGTCTACGCGGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGGGAAGCATTGAAGTGGGAAGACATAAACAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTTCCCCAGTCCAAGGTGCCTTTAGGGAAGCCTTAAACGACTTCGTTTTTAATGGCTTCTACATTCCCAAGGGCTGGAAGCTATATTGGAGTGCAAATGGAACACACAAAAACCCAGAGTACTTCCCAGAGCCGTACAAGTTTGAGCCAGCAAGATTTGAAGGGAATGGGCCATTGCCCTACACTTATGTGCCTTTTGGGGGAGGGCCAAGGATGTGCCCTGGAAAAGAGTATGCAAAGCTTGAGATTTTGGTGTTCATGCATAATTTGGTCAAGAGATTCAAATGGGCAAAGCTACTTGAAAAGGAGAATATCATTGTTGACCCATTGCCAATCCCTCAAAAAGGTCTCCCCGTTCGCCTTTTTCCTCGTCAACCTCTCTCTCTTTAG

Protein sequence

MTNLPPTFFLSPVQNLFLNVTMEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPSSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRFTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQPLSL
Homology
BLAST of CcUC03G053180 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 914.4 bits (2362), Expect = 4.1e-262
Identity = 440/483 (91.10%), Postives = 457/483 (94.62%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           MEHFLISLLI FLFFLSLT FILFH HKSLFSYPNVPPGAIG PILGESLEFLSTGWKG 
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRL+KYKSDVFKTSIVGVPAAIFCG ACNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           STQT+SKEEAKKLKKFLPQFLKPEALQRYIG+MDELAQRHFASFWENREEVLVFPLAK F
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLFVSV+DEI V+RLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKELVA
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IV++R+W LAEGKAS TQDILSHMLLTCDENG +MNESDIADKILGLLIGGHDT SVACT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+KFLAELPH+Y GVY EQMEIAK KAPGEALKWEDI KMKYSWNV CEVLR+ASP QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREAL DFVFNGFYIPKGWKLYWSAN THK+PEYFPEPYKFEPARFEGNGPLPYT+VPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDP+PIPQKGLP+RLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPHQP 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. NCBI nr
Match: XP_008443638.1 (PREDICTED: beta-amyrin 28-oxidase [Cucumis melo])

HSP 1 Score: 896.7 bits (2316), Expect = 8.9e-257
Identity = 431/483 (89.23%), Postives = 450/483 (93.17%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           MEHFLISLLIL  FFLSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           +TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREALNDFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKFEP RFEGNGPLPYT+VPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. NCBI nr
Match: XP_004139087.1 (beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein Csa_007564 [Cucumis sativus])

HSP 1 Score: 888.3 bits (2294), Expect = 3.2e-254
Identity = 427/483 (88.41%), Postives = 449/483 (92.96%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           ME FLISLLIL  FFLSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           +TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFS+ACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREAL+DFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKF+P RFEGNGPLPYT+VPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. NCBI nr
Match: XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.4 bits (2214), Expect = 6.0e-245
Identity = 410/483 (84.89%), Postives = 442/483 (91.51%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           M  FLISLLILFL F+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MADFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+K+LAELP +YD VY EQMEIAKAK  GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFRE +NDFVFNGFYIPKGWKLYWS+N THKN  YFPEPYKF+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KL++KE IIVDPLPIP+KGLPVRLFP + 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHKY 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. NCBI nr
Match: KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 856.3 bits (2211), Expect = 1.3e-244
Identity = 410/483 (84.89%), Postives = 442/483 (91.51%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           M  FLISLLILFL  +SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+K+LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNV CEVLR+ASP+QG
Sbjct: 301 FIVKYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREA+N FVFNGFYIPKGWK+YWS+N THKN  YFPEPYKF+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLL+KE IIVDPLPIP+KGLPVRLFP + 
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHKY 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 2.0e-214
Identity = 345/480 (71.88%), Postives = 415/480 (86.46%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           ME F +SLL++F+  +S+   +LF+KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           STQT+SKEEAKK++K LPQF KPEALQRYIGIMD +AQRHFA  W+NR+EV+VFPLAKRF
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TF LACRLF+S++D   V +  +PF  + +G+I++PIDLPGTPF+RAIKASNFIRKEL A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           I++QRK  LAEGKAS  QDILSHMLL  DE+G +MNE +IADKILGLLIGGHDT S A T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           F++K++AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREA+ DFVFNGF IPKGWKLYWSAN THK+PE FP+P  F+P RFEG+GP PYT+VPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYA+LEILVFMHN+VKRFKW KLL  E IIVDP+P+P KGLPVRL PR+P
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRKP 480

BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 736.5 bits (1900), Expect = 2.0e-211
Identity = 342/480 (71.25%), Postives = 411/480 (85.62%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           ME F +SLL++ +  +S+  ++L +KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           STQT+SKEEAKK++K LPQFLKPEALQRY GIMD +AQRHFA  W+NR+EV+VFPLAKRF
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TF LACRLF+S++D   V +  +PF  + +G+I++PIDLPGTPF+RAIKASNFIRKEL A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           I++QRK  LAE KAS TQDILSHMLL  DE+G +MNE  IADKILGLLIGGHDT S A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           F++K++AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREA+ DFVFNGF IPKGWKLYWSAN THK+ E FP+P KF+P RFEG GP PYT+VPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYA+LEIL+FMHNLVKRFKW KLL  E IIVDP+P+P KGLPVRL P +P
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHKP 480

BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 4.7e-208
Identity = 340/478 (71.13%), Postives = 402/478 (84.10%), Query Frame = 0

Query: 24  HFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPE 83
           +F +SLL+LF+ F+SL+ F +F+K KS     N+PPG +G+PI+GESLEFLSTGWKGHPE
Sbjct: 4   NFYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPE 63

Query: 84  KFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP-SS 143
           KFIFDR+ KY S++FKTSIVG    + CG A NKFLFSNENKLVT+WWPDSVNKIFP +S
Sbjct: 64  KFIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTS 123

Query: 144 TQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRFT 203
             +N KEE+ K++K LPQF KPEALQRY+G+MD +AQRHF + W+N+ E+ V+PLAKR+T
Sbjct: 124 LDSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYT 183

Query: 204 FSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAI 263
           F LACRLF+SV+DE  V + S PF+ I AGIISLPIDLPGTPFN+AIKASNFIRKEL+ I
Sbjct: 184 FLLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKI 243

Query: 264 VRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTF 323
           ++QR+  LAEG ASPTQDILSHMLLT DENG  MNE +IADKILGLLIGGHDT SVACTF
Sbjct: 244 IKQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTF 303

Query: 324 ILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGA 383
           ++K+L ELPH+YD VY EQMEIAK+K  GE L W+D+ KMKYSWNVACEV+R++ P+QG 
Sbjct: 304 LVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGG 363

Query: 384 FREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPFG 443
           FREA+ DF+FNGF IPKGWKLYWSAN THKN E FP P KF+P RFEGNGP PYT+VPFG
Sbjct: 364 FREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFG 423

Query: 444 GGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQ 501
           GGPRMCPGKEYA+LEILVFMHNLVKRFKW K++  E IIVDP PIP K LP+RL+P +
Sbjct: 424 GGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478

BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.4e-207
Identity = 340/480 (70.83%), Postives = 404/480 (84.17%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKS-LFSYPNVPPGAIGFPILGESLEFLSTGWKG 81
           M+ F + LL LF+ F+SL+F+ LF+K KS   S   +PPG  G+P++GES EFLSTGWKG
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 82  HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 141
           +PEKFIFDRL KY S+VFKTS+ G PAA FCG ACNKFLFSNENKLV +WWPDSVNK+FP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 142 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKR 201
           SSTQT+SKEEA K++K LP FLKPEALQRY+G+MD++A +HF S WEN++EVLVFPLAK 
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

Query: 202 FTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 261
           +TF +AC++FVSV++  QV +L  PF  I +GIIS+PIDLPGTPFN AIK+S  +R +L+
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240

Query: 262 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 321
            I++QRK  L EGKASPTQDILSHMLLT DENG +M E DIADKILGLLIGGHDT S AC
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300

Query: 322 TFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQ 381
           TF++KFLAELP +Y+GVY EQMEIAK+K  GE L WEDI KMKYSWNVACEVLR+A P+Q
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360

Query: 382 GAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVP 441
           GAFREAL DF +NGF IPKGWKLYWSAN TH+N E FPEP KF+P+RFEG GP PY++VP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420

Query: 442 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQ 501
           FGGGPRMCPGKEYA+LEILVFMH++VKRFKW K++  E I+V+P+PIP KGLPVRLFP +
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480

BLAST of CcUC03G053180 vs. ExPASy Swiss-Prot
Match: K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 3.7e-205
Identity = 340/478 (71.13%), Postives = 404/478 (84.52%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFH-KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKG 81
           ME F  SL+ LF+  LSL+F  LF+ K+KS+     +PPG  G+P++GE+LEFLSTGWKG
Sbjct: 1   MELFYASLVCLFVLLLSLSFLFLFNKKNKSMILSCPLPPGNSGWPVIGETLEFLSTGWKG 60

Query: 82  HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 141
           HPEKFIFDR++KYKS +FKT ++G  A +FCG + NKFLFSNENKLV +WWP SV+K+FP
Sbjct: 61  HPEKFIFDRISKYKSSIFKTHLLGEKAVVFCGASSNKFLFSNENKLVQTWWPSSVDKVFP 120

Query: 142 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKR 201
           SSTQT+SKEEA K++K LP FLKPEALQRYI IMD +AQ+HF S WEN++EV+VFPLAKR
Sbjct: 121 SSTQTSSKEEAIKMRKMLPNFLKPEALQRYIRIMDHIAQKHFQS-WENQQEVVVFPLAKR 180

Query: 202 FTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 261
           +TF LACRLFVSV+D   V RL+ PF+ + +G+IS+PI+LPGTPFNRAIKASNFIRKELV
Sbjct: 181 YTFWLACRLFVSVEDPNHVARLADPFDVLASGLISIPINLPGTPFNRAIKASNFIRKELV 240

Query: 262 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 321
           AI++QRK  L EGKAS +QDILSHMLLT DENG +M+E DIADKILGLLIGGHDT S AC
Sbjct: 241 AIIKQRKIDLTEGKASDSQDILSHMLLTSDENGKFMHELDIADKILGLLIGGHDTASSAC 300

Query: 322 TFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQ 381
            FI+K+LAELP +YD VY EQ+EIAK+K PGE L WEDI KMKYSWNVACEVLR+A P+Q
Sbjct: 301 AFIVKYLAELPEIYDQVYKEQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQ 360

Query: 382 GAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVP 441
           GAFREAL DF FNGF IPKGWK+YWSAN TH NPE F +P KF+P+RFEGNGP PYT+VP
Sbjct: 361 GAFREALVDFTFNGFSIPKGWKIYWSANSTHINPEVFVDPLKFDPSRFEGNGPAPYTFVP 420

Query: 442 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFP 499
           FGGGPRMCPGKEYA+LEILVFMH+LVKRF W K++  E I+V+P+PIP  GLP+RL+P
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHLVKRFSWKKIIRDEKIVVNPMPIPANGLPIRLYP 477

BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match: A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.3e-257
Identity = 431/483 (89.23%), Postives = 450/483 (93.17%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           MEHFLISLLIL  FFLSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           +TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREALNDFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKFEP RFEGNGPLPYT+VPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match: A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 427/483 (88.41%), Postives = 449/483 (92.96%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           ME FLISLLIL  FFLSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           +TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAK F
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFS+ACRLF+SV+DEI VERLS PFE I AGIIS+PIDLPGTPFNRAIKAS FIRKE+VA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+KFLAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREAL+DFVFNGF+IPKGWKLYWSAN THKNPEYFPEPYKF+P RFEGNGPLPYT+VPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+P+PIPQKGLPVRLFP QP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match: A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.5e-245
Identity = 410/483 (84.89%), Postives = 441/483 (91.30%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           M  FLISLLILFL  +SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+ +LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVNYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREA+N FVFNGFYIPKGWK+YWS+N THKN  YFPEPYKF+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLL+KE IIVDPLPIP+KGLPVRLFP   
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTT 480

Query: 502 LSL 505
           LSL
Sbjct: 481 LSL 483

BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match: A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.1e-243
Identity = 411/485 (84.74%), Postives = 441/485 (90.93%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           ME FLISLLILFL F+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE VLVFPLAK F
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLF+S++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IVRQRK  LAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDT S ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEI--AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 381
           FI+K+LAELP +YD VY EQMEI  AKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 382 QGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYV 441
           QGAFREA+NDFVFNGF IPKGWKLYWS+N THKN  YFPEPYKF+P+RFEGNGP PYT+V
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 442 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPR 501
           PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLEKE IIVDPLPIP+KGLPVRLFP 
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

Query: 502 QPLSL 505
           + LSL
Sbjct: 481 KTLSL 485

BLAST of CcUC03G053180 vs. ExPASy TrEMBL
Match: A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 7.9e-243
Identity = 399/482 (82.78%), Postives = 437/482 (90.66%), Query Frame = 0

Query: 22  MEHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 81
           MEHFL+SLLI F+  + +  FILF+KHKS FSYPN+PPG IGFPI+GESLEF STGWKG 
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 82  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 141
           PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 142 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 201
           STQT+S EEAKKLKK LPQFLKPEAL RYIGIMD LA RHFAS WEN+++VLVFPLAK F
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 202 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 261
           TFSLACRLFVSV+DE  VERLS PF+ I AGIIS+PID PGTPFNR IKASNFIRKEL++
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 262 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 321
           IV+QRK  LAEGKASPTQDILSHMLLTCDENG YMNE DIADKILGLLIGGHDT S ACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 322 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 381
           FI+KFLAELPH+YDGVY EQM IAKAKAPGE L WEDI KMKYSWNVACEVLR+ASP+QG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 382 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 441
           AFREA+NDFVFNGFYIPKGWKLYWS+N THKN +YFP+PY F+P+RFEGNGP PYT+VPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 442 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFPRQP 501
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKWAK+LEKE I+VDP+PIPQKGLPVRLFP + 
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480

Query: 502 LS 504
           +S
Sbjct: 481 VS 482

BLAST of CcUC03G053180 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 638.6 bits (1646), Expect = 4.1e-183
Identity = 301/479 (62.84%), Postives = 367/479 (76.62%), Query Frame = 0

Query: 25  FLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEK 84
           ++  ++ILFL  + L+  +L  KH S FSYPN+PPG  G P++GES  FLS G +GHPEK
Sbjct: 2   YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61

Query: 85  FIFDRLNKYKSD---VFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 144
           FI DR+ ++ S    VFKT + G P A+  G + NKFLF+NENKLV SWWPDSVNKIFPS
Sbjct: 62  FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121

Query: 145 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 204
           S QT+SKEEA+KL+  L QF+KPEAL+RY+G+MDE+AQRHF + W N+++V+VFPL K+F
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 181

Query: 205 TFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVA 264
           TFS+ACR F+S++D  +V +L   F  +  GI S+PIDLPGT FNRAIKAS  +RKE+ A
Sbjct: 182 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 241

Query: 265 IVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACT 324
           IVRQRK  L  GKA    DILSHML+   E      + D+ADKI+GLLIGGHDT S+ CT
Sbjct: 242 IVRQRKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCT 301

Query: 325 FILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQG 384
           F++ +LAE PHVY  V  EQ EI K K   E L+WEDI KM+YSWNVACEV+RI  P+ G
Sbjct: 302 FVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSG 361

Query: 385 AFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYVPF 444
            FREA++ F F GFYIPKGWKLYWSA  TH NP+YFPEP +FEP RFEG+GP PYTYVPF
Sbjct: 362 TFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPF 421

Query: 445 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKEN-IIVDPLPIPQKGLPVRLFPR 500
           GGGPRMCPGKEYA+LEIL+FMHNLV RFKW K+   EN I+VDPLPIP KGLP+R+FP+
Sbjct: 422 GGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 476

BLAST of CcUC03G053180 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 377.1 bits (967), Expect = 2.2e-104
Identity = 184/318 (57.86%), Postives = 237/318 (74.53%), Query Frame = 0

Query: 29  LLILFLFFLSLTFFILFH--KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 88
           L I+FLF  S+ F +LF   KH S F YPN+PPG IGFP++GE+L FLS G +GHPEKF+
Sbjct: 3   LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62

Query: 89  FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPSSTQTN 148
            DR+  + S +FKT + G P A+  G + NKFLF+NENKLV SWWPDSVNKIFPSSTQT+
Sbjct: 63  TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122

Query: 149 SKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRFTFSLA 208
           SKEEA K +  L   +KPEAL+RY+G+MDE+AQ+HF + W N+++++VFPL K+FTFS+A
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182

Query: 209 CRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 268
           CRLF+S+DD  +V +L  PF  ++ G+ S+PIDLPGT FNRAIKAS  + KE+  I+RQR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242

Query: 269 KWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 328
           K  L  GK S  QDILSHML+   E      + D+ADKI+ LLIGGHDTTS+ CTF++ +
Sbjct: 243 KEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNY 302

Query: 329 LAELPHVYDGVYAEQMEI 345
           LAE PH+Y  V  E M+I
Sbjct: 303 LAEFPHIYQRV-LEGMQI 315

BLAST of CcUC03G053180 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 279.3 bits (713), Expect = 6.3e-75
Identity = 165/486 (33.95%), Postives = 261/486 (53.70%), Query Frame = 0

Query: 24  HFLISLLILFL-FFLSLTFFILFHKH-----KSLFSYPNVPPGAIGFPILGESLEFLSTG 83
           +F++S + L +   +S T F    KH     K +     + PG +G P +GE+++F    
Sbjct: 6   NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65

Query: 84  WKGHP-EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWP-DSV 143
                 E F+  R+ K+  ++FKT I+G P  +  G   N+ + SNE  LV S WP  SV
Sbjct: 66  KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125

Query: 144 NKIFPSSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVF 203
             +  +       E+ + L+  +   L    L+  I  + +  + H  + W  +EE+ ++
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185

Query: 204 PLAKRFTFSLACRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFI 263
             AK  TF++       +  EI +  +   FE+++ G+ +LP++ P + F RA KA   I
Sbjct: 186 RSAKVLTFTVVFECLYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEI 245

Query: 264 RKELVAIVRQ-RKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHD 323
              LV  VR+ R+ +  EG   P   + S ++    +    + E ++ D ++ L+   HD
Sbjct: 246 ETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELIKG--VITEEEVVDNMVLLVFAAHD 305

Query: 324 TTSVACTFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLR 383
           TTS A +   K LA+ P   D +  E  +I   K  GE L  ED+ KMKYSW V  E +R
Sbjct: 306 TTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMR 365

Query: 384 IASPVQGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPL 443
           ++ P+ G+FR+A+ D  + G+ IPKGWK+ W+  GTH NPE F +P  F+P RF+   P+
Sbjct: 366 LSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFD--KPI 425

Query: 444 -PYTYVPFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLP 500
             YTY+PFGGGPR+C G + AK+ ILVFMH +V  F W+ +   E I +DPLP P  G+P
Sbjct: 426 QAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMP 483

BLAST of CcUC03G053180 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 254.6 bits (649), Expect = 1.7e-67
Identity = 153/479 (31.94%), Postives = 254/479 (53.03%), Query Frame = 0

Query: 23  EHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHP 82
           E  LI+L I+ +  + L  + L  K + L     +PPG++G P +GE+L      +  +P
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENP 78

Query: 83  EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNK-IFPS 142
             F   R NKY  D+FKT I+G P  +   P   + +  ++  L    +P S  + I P 
Sbjct: 79  NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138

Query: 143 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 202
           +   +       LK+ +     P AL+  +  ++ L  +  +S W +++ +      KR+
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSS-WTSQKSINTLEYMKRY 198

Query: 203 TFSLA-CRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 262
            F +A    F   ++   ++ +   ++++  G  S+P+DLPGT F++++KA   + +EL 
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258

Query: 263 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 322
            ++ +R+             +L  +L   D+    +++S IAD I+G++    DTT+   
Sbjct: 259 KVIEKRR-----ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 323 TFILKFLAELPHVYDGVYAEQMEI-AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 382
           T++LK+L + P++   V  EQ  I  K K     + WED  KM  +  V  E LR AS +
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 383 QGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYV 442
              FREA+ D  ++G+ IPKGWK+       H + E+FP+P KF+P+RFE   P PYTY+
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438

Query: 443 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFP 499
           PFG G   CPG E AKLE+L+ +H+L   F+W  + ++E I   P P+P+KGLP+R+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

BLAST of CcUC03G053180 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 254.6 bits (649), Expect = 1.7e-67
Identity = 153/479 (31.94%), Postives = 254/479 (53.03%), Query Frame = 0

Query: 23  EHFLISLLILFLFFLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHP 82
           E  LI+L I+ +  + L  + L  K + L     +PPG++G P +GE+L      +  +P
Sbjct: 19  EPALITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRL----YTENP 78

Query: 83  EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNK-IFPS 142
             F   R NKY  D+FKT I+G P  +   P   + +  ++  L    +P S  + I P 
Sbjct: 79  NSFFATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPE 138

Query: 143 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKRF 202
           +   +       LK+ +     P AL+  +  ++ L  +  +S W +++ +      KR+
Sbjct: 139 ALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSS-WTSQKSINTLEYMKRY 198

Query: 203 TFSLA-CRLFVSVDDEIQVERLSRPFEQIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 262
            F +A    F   ++   ++ +   ++++  G  S+P+DLPGT F++++KA   + +EL 
Sbjct: 199 AFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 258

Query: 263 AIVRQRKWVLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVAC 322
            ++ +R+             +L  +L   D+    +++S IAD I+G++    DTT+   
Sbjct: 259 KVIEKRR-----ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVL 318

Query: 323 TFILKFLAELPHVYDGVYAEQMEI-AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 382
           T++LK+L + P++   V  EQ  I  K K     + WED  KM  +  V  E LR AS +
Sbjct: 319 TWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVL 378

Query: 383 QGAFREALNDFVFNGFYIPKGWKLYWSANGTHKNPEYFPEPYKFEPARFEGNGPLPYTYV 442
              FREA+ D  ++G+ IPKGWK+       H + E+FP+P KF+P+RFE   P PYTY+
Sbjct: 379 SFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYM 438

Query: 443 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPLPIPQKGLPVRLFP 499
           PFG G   CPG E AKLE+L+ +H+L   F+W  + ++E I   P P+P+KGLP+R+ P
Sbjct: 439 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877759.14.1e-26291.10LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida][more]
XP_008443638.18.9e-25789.23PREDICTED: beta-amyrin 28-oxidase [Cucumis melo][more]
XP_004139087.13.2e-25488.41beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein ... [more]
XP_023516848.16.0e-24584.89beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo][more]
KAG6590124.11.3e-24484.89Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
F6H9N62.0e-21471.88Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1[more]
A5BFI42.0e-21171.25Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2[more]
Q2MJ204.7e-20871.13Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... [more]
A0A0S2II381.4e-20770.83Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... [more]
K4CEE83.7e-20571.13Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A1S3B8H44.3e-25789.23beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1[more]
A0A0A0LXN31.5e-25488.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1[more]
A0A6J1H8T48.5e-24584.89beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1[more]
A0A6J1JJQ72.1e-24384.74beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1[more]
A0A6J1DZX77.9e-24382.78beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36110.14.1e-18362.84cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT5G36140.12.2e-10457.86cytochrome P450, family 716, subfamily A, polypeptide 2 [more]
AT2G42850.16.3e-7533.95cytochrome P450, family 718 [more]
AT2G29090.11.7e-6731.94cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.21.7e-6731.94cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 311..328
score: 33.37
coord: 439..448
score: 58.31
coord: 448..459
score: 35.55
IPR001128Cytochrome P450PFAMPF00067p450coord: 58..473
e-value: 2.5E-57
score: 194.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 300..317
score: 28.51
coord: 438..448
score: 49.89
coord: 406..430
score: 27.16
coord: 448..471
score: 28.06
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 48..497
e-value: 9.2E-112
score: 376.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 51..499
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 29..497
NoneNo IPR availablePANTHERPTHR24286:SF297BETA-AMYRIN 28-OXIDASE-LIKEcoord: 29..497
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 441..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CcUC03G053180.1CcUC03G053180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen