Carg27890 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27890
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
LocationCarg_Chr10: 9334591 .. 9335144 (+)
RNA-Seq ExpressionCarg27890
SyntenyCarg27890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCCTTGTTGGGATCTATGAGAGCATGGGACACAGTGGTGTATGAGTTCAAGGAGGAGGAGATGAAGAAGGAGAAGAAGGCGTTATTCACAATCCACCAAAGAGTAGATGGCACCACGTTTGAGTTGATCGTCCATGCAAAATCTTCTGAGAATGCTTGGGAGGTCTGGAGAAAGACTTTGATGGTTTAGATAGAGTGAAGAAAATCTGCTTGCAATATTTACCCGTAGATTTTGAGGCTCTACATCAAGAAGATTCAACATCAATATGTGTTTACTTCCATTGTGTAAACTCCATTGTGTAAGAAATGAGAAGACATGACGAGAAGGTAGAAGAAGTACGAGTTGTGGAGAAGATTATGTGATTTTTACACTCAAGGTTTGACTACATTGCTGTGGAAGTTGAAGAATCTAATAAACTTGAAGAAATATTAGTAGAGGAGCTTCAAGACTCTATACAAGTCTATGAAGAAAGAGGCTTAGAGAAAGAAGAAAGAAGACGTATGGAGCAAGCACTGCAAGCCAACTCTTCCCAAAAGATGGATGGCTAG

mRNA sequence

ATGGAGGCCTTGTTGGGATCTATGAGAGCATGGGACACAGTGGTGTATGAGTTCAAGGAGGAGGAGATGAAGAAGGAGAAGAAGGCGTTATTCACAATCCACCAAAGAGTAGATGGCACCACGTTTGAGTTGATCGTCCATGCAAAATCTTCTGAGAATGCTTGGGAGATTTTGAGGCTCTACATCAAGAAGATTCAACATCAATATGTGTTTACTTCCATTGTGTTTGACTACATTGCTGTGGAAGTTGAAGAATCTAATAAACTTGAAGAAATATTAGTAGAGGAGCTTCAAGACTCTATACAAGTCTATGAAGAAAGAGGCTTAGAGAAAGAAGAAAGAAGACGTATGGAGCAAGCACTGCAAGCCAACTCTTCCCAAAAGATGGATGGCTAG

Coding sequence (CDS)

ATGGAGGCCTTGTTGGGATCTATGAGAGCATGGGACACAGTGGTGTATGAGTTCAAGGAGGAGGAGATGAAGAAGGAGAAGAAGGCGTTATTCACAATCCACCAAAGAGTAGATGGCACCACGTTTGAGTTGATCGTCCATGCAAAATCTTCTGAGAATGCTTGGGAGATTTTGAGGCTCTACATCAAGAAGATTCAACATCAATATGTGTTTACTTCCATTGTGTTTGACTACATTGCTGTGGAAGTTGAAGAATCTAATAAACTTGAAGAAATATTAGTAGAGGAGCTTCAAGACTCTATACAAGTCTATGAAGAAAGAGGCTTAGAGAAAGAAGAAAGAAGACGTATGGAGCAAGCACTGCAAGCCAACTCTTCCCAAAAGATGGATGGCTAG

Protein sequence

MEALLGSMRAWDTVVYEFKEEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRLYIKKIQHQYVFTSIVFDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRMEQALQANSSQKMDG
Homology
BLAST of Carg27890 vs. NCBI nr
Match: KAG7023994.1 (hypothetical protein SDJN02_15022, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 247.3 bits (630), Expect = 7.4e-62
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0

Query: 1   MEALLGSMRAWDTVVYEFKEEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRL 60
           MEALLGSMRAWDTVVYEFKEEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRL
Sbjct: 1   MEALLGSMRAWDTVVYEFKEEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRL 60

Query: 61  YIKKIQHQYVFTSIVFDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRMEQA 120
           YIKKIQHQYVFTSIVFDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRMEQA
Sbjct: 61  YIKKIQHQYVFTSIVFDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRMEQA 120

Query: 121 LQANSSQKMDG 132
           LQANSSQKMDG
Sbjct: 121 LQANSSQKMDG 131

BLAST of Carg27890 vs. NCBI nr
Match: XP_042418650.1 (uncharacterized protein LOC122006990 [Zingiber officinale])

HSP 1 Score: 72.4 bits (176), Expect = 3.3e-09
Identity = 65/195 (33.33%), Postives = 88/195 (45.13%), Query Frame = 0

Query: 1   MEALLGSMRAWDTV-----VYEFKEEE-------MKKEKKALFTIHQRVDGTTFELIVHA 60
           ME LLGS+  WD V     + E  +E         K++KK LFTI+Q +D   FELI  A
Sbjct: 24  MEVLLGSLDVWDLVETGYTIAETSDENEQALKAMKKRKKKVLFTIYQGIDEAAFELIAVA 83

Query: 61  KSSENAWEILRL------YIKKIQHQY------------------VFTSIV--------- 120
            +S+ AWEIL+        +KKI+ Q                    F+ ++         
Sbjct: 84  TTSQEAWEILKTAYDGVDKVKKIRLQARRAQFEALQQEAPEIISDYFSRVISIVHQMRRN 143

Query: 121 --------------------FDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERR 131
                               FD+I V +EES  LE + VE L  S+Q YE+R L+K E R
Sbjct: 144 GEELKDVRVVEKILRSLDSKFDFIVVAIEESKDLEVMAVEVLMGSLQTYEQRILKKSEER 203

BLAST of Carg27890 vs. NCBI nr
Match: XP_019419448.1 (PREDICTED: uncharacterized protein LOC109329946 [Lupinus angustifolius])

HSP 1 Score: 72.0 bits (175), Expect = 4.3e-09
Identity = 47/125 (37.60%), Postives = 80/125 (64.00%), Query Frame = 0

Query: 20  EEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEI----------LRLYIKKIQHQY 79
           +E  KK+ KA+F +HQ VD   FE I  A++S+ AW+I          L+ +++K ++Q+
Sbjct: 60  KESKKKDCKAMFLLHQCVDEAHFEKISGAENSKEAWKILEACNQGVEQLKKHVEK-RYQF 119

Query: 80  VFTSIV------FDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRMEQALQA 129
           +   I+      FD+I V +EES +LEE+ VE+LQ S++ +E+R +E+   R +EQALQA
Sbjct: 120 IVEKILRTLTPNFDHIVVAIEESKRLEEMKVEDLQGSLEAHEQRLMERAIERPVEQALQA 179

BLAST of Carg27890 vs. NCBI nr
Match: KHN11212.1 (hypothetical protein glysoja_034441, partial [Glycine soja])

HSP 1 Score: 68.2 bits (165), Expect = 6.1e-08
Identity = 44/100 (44.00%), Postives = 63/100 (63.00%), Query Frame = 0

Query: 21  EEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRLYIKKIQHQYVFTSI--VFDY 80
           E  KK+  A+  IHQ VD   FE IV A +S+ AW+IL    +K   + +  ++   FD+
Sbjct: 10  ENKKKDCNAIVLIHQCVDDAHFEKIVGAATSQEAWKIL----EKCNEEKILRTLNPKFDH 69

Query: 81  IAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRME 119
           I V +EES KLEEI VEELQ S++V+E+R +E+E  +  E
Sbjct: 70  IVVAIEESKKLEEITVEELQGSLEVHEQRLIEREIEKPRE 105

BLAST of Carg27890 vs. NCBI nr
Match: XP_019462948.1 (PREDICTED: uncharacterized protein LOC109361872 [Lupinus angustifolius])

HSP 1 Score: 67.4 bits (163), Expect = 1.0e-07
Identity = 47/131 (35.88%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 1   MEALLGSMRAWDTVVYEFKEEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRL 60
           ++ + G M   D    E++E   K++ KA+F IHQ VD   FE I  A SS+ AWEIL  
Sbjct: 36  LDVVKGGMPTVDENSMEYREAN-KRDCKAIFLIHQCVDRANFEKISAASSSKEAWEILEK 95

Query: 61  YI---KKIQHQYVFTSIVFDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRM 120
                KKI+         FD++   +EE+  L+ + V+ELQ S++V+E+R +E++  R+ 
Sbjct: 96  NYSGGKKIKKILRTLHPRFDHVVAVIEEAKDLDNLSVDELQGSLEVHEQRFMERQNYRQT 155

Query: 121 EQALQANSSQK 129
           + AL A   +K
Sbjct: 156 DHALSAQFKKK 165

BLAST of Carg27890 vs. ExPASy TrEMBL
Match: A0A0B2PUL3 (Uncharacterized protein (Fragment) OS=Glycine soja OX=3848 GN=glysoja_034441 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.0e-08
Identity = 44/100 (44.00%), Postives = 63/100 (63.00%), Query Frame = 0

Query: 21  EEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILRLYIKKIQHQYVFTSI--VFDY 80
           E  KK+  A+  IHQ VD   FE IV A +S+ AW+IL    +K   + +  ++   FD+
Sbjct: 10  ENKKKDCNAIVLIHQCVDDAHFEKIVGAATSQEAWKIL----EKCNEEKILRTLNPKFDH 69

Query: 81  IAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRME 119
           I V +EES KLEEI VEELQ S++V+E+R +E+E  +  E
Sbjct: 70  IVVAIEESKKLEEITVEELQGSLEVHEQRLIEREIEKPRE 105

BLAST of Carg27890 vs. ExPASy TrEMBL
Match: A0A6A2XI87 (CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00117010pilonHSYRG00118 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-07
Identity = 47/138 (34.06%), Postives = 72/138 (52.17%), Query Frame = 0

Query: 1   MEALLGSMRAWDTVVYEFKE-------------------EEMKKEKKALFTIHQRVDGTT 60
           M+ALLGS   WD +   + E                   +  K++KKAL+ I+Q +D   
Sbjct: 25  MKALLGSQDVWDIIEKGYNEHTNDDAFATLTPDQKTTLKDSRKRDKKALYLIYQALDDDG 84

Query: 61  FELIVHAKSSENAWEILRLYIKKIQHQYVFTSIV------FDYIAVEVEESNKLEEILVE 114
           FE I  A SS+ AWE  +L  +K+    +   I+      F++I V +EE+  LEE+ +E
Sbjct: 85  FEKISSASSSKEAWE--KLNGEKLGDVRIIEKILRSLDPKFEHIVVTIEETKDLEEMKIE 144

BLAST of Carg27890 vs. ExPASy TrEMBL
Match: A0A6A3ASR7 (TMV resistance protein N-like OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110377pilonHSYRG00342 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.9e-07
Identity = 47/150 (31.33%), Postives = 74/150 (49.33%), Query Frame = 0

Query: 1   MEALLGSMRAWDTVVYEFKE-------------------EEMKKEKKALFTIHQRVDGTT 60
           M+AL+GS   WD +   + E                   +  K++KKAL+ I+Q +D   
Sbjct: 229 MKALIGSQDVWDIIEKGYNEPADDDSFATLIPDQKTTLKDSRKRDKKALYLIYQALDDDG 288

Query: 61  FELIVHAKSSENAWEILRLY------IKKIQHQYVFTSI------------VFDYIAVEV 114
           FE I  A SS+ AWE L+ +      +KK++ Q +   +             F++I V +
Sbjct: 289 FEKISSASSSKEAWEKLQTFYKGSEQVKKVRLQTLRDDVRIIEKILRLFDPKFEHIVVTI 348

BLAST of Carg27890 vs. ExPASy TrEMBL
Match: A0A392NYH4 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_0025357 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-07
Identity = 50/127 (39.37%), Postives = 72/127 (56.69%), Query Frame = 0

Query: 20  EEEMKKEKKALFTIHQRVDGTTFELIVHAKSSENAWEILR------LYIKKIQHQ----- 79
           +E  KK+ KALF +HQ +D   FE I HA  +++AW+IL         +KK+Q Q     
Sbjct: 49  KELKKKDCKALFILHQCMDAANFEKIAHATYAKDAWKILENSYEGAAKLKKVQLQTLMED 108

Query: 80  -----YVFTSIV--FDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERRRMEQAL 129
                 V  S+   FDYI V +EES  L  + VEELQ S++ +E+R  E+   RR ++ L
Sbjct: 109 QTIVEKVLRSLTQKFDYIVVAIEESKDLGCMKVEELQASLEAHEQRMNERTSDRRNDREL 168

BLAST of Carg27890 vs. ExPASy TrEMBL
Match: A0A6I9RF49 (uncharacterized protein LOC105048038 OS=Elaeis guineensis var. tenera OX=51953 GN=LOC105048038 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.3e-07
Identity = 59/193 (30.57%), Postives = 86/193 (44.56%), Query Frame = 0

Query: 1   MEALLGSMRAWDTVVYEFKE------------EEMKKEKKALFTIHQRVDGTTFELIVHA 60
           M+ALLGS   WD V   ++E            E  K++KKAL+ I+Q +D + FE I  A
Sbjct: 1   MKALLGSQDLWDLVEEGYQEPREGEALTNQQRESRKRDKKALYFIYQSIDESAFEKIAVA 60

Query: 61  KSSENAWEILRLYIKKI---------------------QHQYV---FTSIV--------- 120
            +S+ AWEIL+   K++                     + +YV   FT ++         
Sbjct: 61  TTSKQAWEILQNSYKEVEKVKKVHLQTLRGEFEALQMKESEYVLDYFTRVLAVVNQLRRN 120

Query: 121 --------------------FDYIAVEVEESNKLEEILVEELQDSIQVYEERGLEKEERR 129
                               F+YI V +EES  LEE+ ++EL  S+Q +EER   K  R 
Sbjct: 121 GKTMDDTRVIEKILRSLDVKFNYIVVAIEESKDLEEMTIDELMGSLQAHEER-FNKRRRE 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023994.17.4e-62100.00hypothetical protein SDJN02_15022, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_042418650.13.3e-0933.33uncharacterized protein LOC122006990 [Zingiber officinale][more]
XP_019419448.14.3e-0937.60PREDICTED: uncharacterized protein LOC109329946 [Lupinus angustifolius][more]
KHN11212.16.1e-0844.00hypothetical protein glysoja_034441, partial [Glycine soja][more]
XP_019462948.11.0e-0735.88PREDICTED: uncharacterized protein LOC109361872 [Lupinus angustifolius][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0B2PUL33.0e-0844.00Uncharacterized protein (Fragment) OS=Glycine soja OX=3848 GN=glysoja_034441 PE=... [more]
A0A6A2XI871.1e-0734.06CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig0... [more]
A0A6A3ASR71.9e-0731.33TMV resistance protein N-like OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig0011037... [more]
A0A392NYH42.5e-0739.37Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Trifol... [more]
A0A6I9RF494.3e-0730.57uncharacterized protein LOC105048038 OS=Elaeis guineensis var. tenera OX=51953 G... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 87..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27890-RACarg27890-RAmRNA