Carg27333 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27333
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTHO complex, subunit
LocationCarg_Chr09: 1001286 .. 1004274 (-)
RNA-Seq ExpressionCarg27333
SyntenyCarg27333
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGGAATGCTCATAGAAGACGAGTCCGAGCCCCCGCCGCCGGACAGCGATTCGGGCAAAATGTCTCCCTACGAAATGCTGCGAGAGAGTAAAATTTCTGTGGAGGAGATCGTCGCCAAGATGCTCTCTATCAAGAAGCAAGGCGAGCCTAAAACTCAGCTCGGAGAACTCGTTACGCAGATGCTCCTTCACTTCGTAACGCTTCGCCAGGTTCTTATTTCTATCCGCTTTCTCTTTTAGGGTTGTAGGTTATTTTCAAGATTCATTGGTTCGAAATGGTCTTTTTTTCCTTGCTAATTTGCCATGCGGCGCCACGAACTACAATTATACTGTTGTAGATTTCAATTTTAAATTGCAGCCATTGATGACTCTCGTGGCATAATGTCTGTTCACAGGCCAACCGCTCTATTTTGCTGGAAGAGGACCGAGTGAAAGCTGAAACAGAGAGAGCAAAAGCTCCAGTTGACTTCACGACTCTGCAACTTAACAACTTGATGTATGAAAAGAGTCACTATGTTAAAGCAATAAAGGCCTGTAAAGACTTCAAATCAAAGTATTCTGATAGTGAACTTGTGTCTGAGGATGAGTTTTTCCGGGATGCACCCGAAGACATTAAGAATTCCACAAGGTCGAAGGACAGCGCGCATAATCTGATGCTGCAGAGGCTTCACTTCGAGCTTTTCCAGGTGATCTTTAGTTGTCGTTCATACTCTCTTTGAATTAATTAGCACGGATGCAGAATTTTTCTGGTAATTAAAGCTACCAATAACTCGGTTAGGACGGACAAAAGCAATAGCTTCAAGTTATATACAAAATTTTGAGGAGGCATCACCTTCCTGTGGTTCTAAAAAAAATTGGTGACTCCATTGGCTTTTACTACTATTTTTAAGAATTGCGGGAAATATAGCATCTTGTGCGTCCTTAACTACTTGCATAAGACATCCTAAACATTATAATTTGAATTATGACGTCAAATTCACCTCGTAACTGAGCTTAAATCTTTCCCTTTTTAGAATGGGTGAATCTATGATGTTTTCCATGTTTTTGGTTTTTATTTGAATGTTCCAATTTTCCACCTTTTGGTCGTGGATGTATAGTTGATTCTTTTTTAGTGATTTTTTTCATTGTTGCTATTGAGTGTATTAATAATATCCATCATTTTACCAGCGCAAAGAACTTCGCAAACGGCGAGATGAACTTGAACAACATAAGAAAAGCCTTCTTGAAGTTATTGCTAATAGAAAGAAATTCATGTCAAGTCTTCCCTCACACCTCAAGTCCTTGAAAAAGGCTTCCTTGCCTGTACAGAATCAGTTGGGAATATTGCAGACAAAGAAATTAAAGCAACACCAACTAGCAGAGCTGCTTCCACCTCCTCTTTATGTAATCTACTCACAATTCTTGGCACAAAAGGAAGCATTTAATGAAAGCATTGAGTTGGAAATAGTAGGAATTGTCAAAGATGCTCAAGCTTTTGCACGCCATCAAGCAAACGAGGAAACTGGTACCTGGGTGTATATCTTCTCTCGACCATTAGTTGAATAGTTATATCATATATGATAGTATTTTTATTGTCAGATTGTTTTAATTTTAGATAGTAGAATTGGATGCATATGCTACAAAATGACTTCTTTTTAAATCATCTTGTCACGCAATACCCTATACATACGAATTGAATTTATTATTCATTGAAATCATCACACAATTCTAAATCCCAGACCATATTTATCTTATAAATAATGTCAATTTGGAGATAACTGAACACTACTTTTTTTGGATTCAAGAGTCTATATCAAAAACAGCATTCACTGATAACTCTTTCTTCATTTATTTTTTTCTTTTTTTTCCTGTAAGAGAGTAATTCTTTCATTGCAAATATTTTAGGTGCATCAACCAATGCTGAGAACTCCAAATTGGAGGATGATGCCCTTGACGAAGATGATGATGGCCAAAGGAGGAAAAAACGGCCAAAGAAGATTCCAGCTAAGGTGAACATTGAGCATGCAGGGATATATCAAGTTCATCCGCTAAAGATCATCCTACATATATATGACAATGAAACCTGTGACAAGTCAATGAAATTGCTTTCTGTGAAGTTTGAATGCCTGTTAAAGTTGAACATTATTTGTGTTGGGATTGAAGGATCTCATGAAGGACCTGAGAATAACATCTTATGCAGCTTGTTCCCTGATGACACTGGCCTTGAGCTGCCTCACCAGGTATGAATTCAGAAATTTGCGTTTATGTTCTTATTGTTCTGCCCCAACTTTACATTTTCAACGAAAAAGAGGGGGAAACCTTGATGATATATTATTTTGTCTTAAATGATGCAGTCAGCCAAGCTTGTTGTTGGTGAAACTCTTGCATTTAGTGACAAGAGAACCTCTCGACCATATAAGTGGGCTCAACATTTGGCTGGAATTGATTTTTTGCCAGAATTGCCACCCCTGGTGAGTGCACAAGAATCTGCCAGTGGTGAAACTGTTAGAAGTGATATTGTATCAGGCCTCTCAATGTATCGTCAGCAAATCCGAATACAGACAGTTGTGCAAAGATTACGCTCTCAGAAAAAGGCTCAGCTTGCTCTTGTGTGAGTCATTGATTTGTATTCAAAGTTTTAGATTATAAATGGCTTTGAAGACTGATAGAAGATGGATTTGTAGCATAGTGATCAAATTGACCATCTGTTGTTTCTCTGATCACTTGCAATTTCATGCACTATGCTTGTGTGATTAACACTTTATTTTCTCCAGAAAACTATATATGCCATGTTTACTTTTACTTCTACAGCATTTTATATAATGGAATATTTTTCCGTTGATGGAAGGATCACTGATTCAGCCAAGTATGGTTTGGTTTATGTTGAGAAATTGGGATTTTTCTACTGACTAACAGGGAACAGCTTGATTCACTTGAAAAACTTAAATGGTCAGTTCTGACATGTTATGATGTCTCATGGGTTTGGCATCAACCTTCATGCTGTTTGTAA

mRNA sequence

GAGGGAATGCTCATAGAAGACGAGTCCGAGCCCCCGCCGCCGGACAGCGATTCGGGCAAAATGTCTCCCTACGAAATGCTGCGAGAGAGTAAAATTTCTGTGGAGGAGATCGTCGCCAAGATGCTCTCTATCAAGAAGCAAGGCGAGCCTAAAACTCAGCTCGGAGAACTCGTTACGCAGATGCTCCTTCACTTCGTAACGCTTCGCCAGGCCAACCGCTCTATTTTGCTGGAAGAGGACCGAGTGAAAGCTGAAACAGAGAGAGCAAAAGCTCCAGTTGACTTCACGACTCTGCAACTTAACAACTTGATGTATGAAAAGAGTCACTATGTTAAAGCAATAAAGGCCTGTAAAGACTTCAAATCAAAGTATTCTGATAGTGAACTTGTGTCTGAGGATGAGTTTTTCCGGGATGCACCCGAAGACATTAAGAATTCCACAAGGTCGAAGGACAGCGCGCATAATCTGATGCTGCAGAGGCTTCACTTCGAGCTTTTCCAGCGCAAAGAACTTCGCAAACGGCGAGATGAACTTGAACAACATAAGAAAAGCCTTCTTGAAGTTATTGCTAATAGAAAGAAATTCATGTCAAGTCTTCCCTCACACCTCAAGTCCTTGAAAAAGGCTTCCTTGCCTGTACAGAATCAGTTGGGAATATTGCAGACAAAGAAATTAAAGCAACACCAACTAGCAGAGCTGCTTCCACCTCCTCTTTATGTAATCTACTCACAATTCTTGGCACAAAAGGAAGCATTTAATGAAAGCATTGAGTTGGAAATAGTAGGAATTGTCAAAGATGCTCAAGCTTTTGCACGCCATCAAGCAAACGAGGAAACTGGTGCATCAACCAATGCTGAGAACTCCAAATTGGAGGATGATGCCCTTGACGAAGATGATGATGGCCAAAGGAGGAAAAAACGGCCAAAGAAGATTCCAGCTAAGGTGAACATTGAGCATGCAGGGATATATCAAGTTCATCCGCTAAAGATCATCCTACATATATATGACAATGAAACCTGTGACAAGTCAATGAAATTGCTTTCTGTGAAGTTTGAATGCCTGTTAAAGTTGAACATTATTTGTGTTGGGATTGAAGGATCTCATGAAGGACCTGAGAATAACATCTTATGCAGCTTGTTCCCTGATGACACTGGCCTTGAGCTGCCTCACCAGTCAGCCAAGCTTGTTGTTGGTGAAACTCTTGCATTTAGTGACAAGAGAACCTCTCGACCATATAAGTGGGCTCAACATTTGGCTGGAATTGATTTTTTGCCAGAATTGCCACCCCTGGTGAGTGCACAAGAATCTGCCAGTGGTGAAACTGTTAGAAGTGATATTGTATCAGGCCTCTCAATGTATCGTCAGCAAATCCGAATACAGACAGTTGTGCAAAGATTACGCTCTCAGAAAAAGGCTCAGCTTGCTCTTGTGGAACAGCTTGATTCACTTGAAAAACTTAAATGGTCAGTTCTGACATGTTATGATGTCTCATGGGTTTGGCATCAACCTTCATGCTGTTTGTAA

Coding sequence (CDS)

ATGCTCATAGAAGACGAGTCCGAGCCCCCGCCGCCGGACAGCGATTCGGGCAAAATGTCTCCCTACGAAATGCTGCGAGAGAGTAAAATTTCTGTGGAGGAGATCGTCGCCAAGATGCTCTCTATCAAGAAGCAAGGCGAGCCTAAAACTCAGCTCGGAGAACTCGTTACGCAGATGCTCCTTCACTTCGTAACGCTTCGCCAGGCCAACCGCTCTATTTTGCTGGAAGAGGACCGAGTGAAAGCTGAAACAGAGAGAGCAAAAGCTCCAGTTGACTTCACGACTCTGCAACTTAACAACTTGATGTATGAAAAGAGTCACTATGTTAAAGCAATAAAGGCCTGTAAAGACTTCAAATCAAAGTATTCTGATAGTGAACTTGTGTCTGAGGATGAGTTTTTCCGGGATGCACCCGAAGACATTAAGAATTCCACAAGGTCGAAGGACAGCGCGCATAATCTGATGCTGCAGAGGCTTCACTTCGAGCTTTTCCAGCGCAAAGAACTTCGCAAACGGCGAGATGAACTTGAACAACATAAGAAAAGCCTTCTTGAAGTTATTGCTAATAGAAAGAAATTCATGTCAAGTCTTCCCTCACACCTCAAGTCCTTGAAAAAGGCTTCCTTGCCTGTACAGAATCAGTTGGGAATATTGCAGACAAAGAAATTAAAGCAACACCAACTAGCAGAGCTGCTTCCACCTCCTCTTTATGTAATCTACTCACAATTCTTGGCACAAAAGGAAGCATTTAATGAAAGCATTGAGTTGGAAATAGTAGGAATTGTCAAAGATGCTCAAGCTTTTGCACGCCATCAAGCAAACGAGGAAACTGGTGCATCAACCAATGCTGAGAACTCCAAATTGGAGGATGATGCCCTTGACGAAGATGATGATGGCCAAAGGAGGAAAAAACGGCCAAAGAAGATTCCAGCTAAGGTGAACATTGAGCATGCAGGGATATATCAAGTTCATCCGCTAAAGATCATCCTACATATATATGACAATGAAACCTGTGACAAGTCAATGAAATTGCTTTCTGTGAAGTTTGAATGCCTGTTAAAGTTGAACATTATTTGTGTTGGGATTGAAGGATCTCATGAAGGACCTGAGAATAACATCTTATGCAGCTTGTTCCCTGATGACACTGGCCTTGAGCTGCCTCACCAGTCAGCCAAGCTTGTTGTTGGTGAAACTCTTGCATTTAGTGACAAGAGAACCTCTCGACCATATAAGTGGGCTCAACATTTGGCTGGAATTGATTTTTTGCCAGAATTGCCACCCCTGGTGAGTGCACAAGAATCTGCCAGTGGTGAAACTGTTAGAAGTGATATTGTATCAGGCCTCTCAATGTATCGTCAGCAAATCCGAATACAGACAGTTGTGCAAAGATTACGCTCTCAGAAAAAGGCTCAGCTTGCTCTTGTGGAACAGCTTGATTCACTTGAAAAACTTAAATGGTCAGTTCTGACATGTTATGATGTCTCATGGGTTTGGCATCAACCTTCATGCTGTTTGTAA

Protein sequence

MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQMLLHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQRRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICVGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGIDFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDSLEKLKWSVLTCYDVSWVWHQPSCCL
Homology
BLAST of Carg27333 vs. NCBI nr
Match: KAG7024259.1 (THO complex subunit 5B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 973.4 bits (2515), Expect = 7.5e-280
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML
Sbjct: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK
Sbjct: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ
Sbjct: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICV 360
           RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICV
Sbjct: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICV 360

Query: 361 GIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGID 420
           GIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGID
Sbjct: 361 GIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGID 420

Query: 421 FLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDS 480
           FLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDS
Sbjct: 421 FLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDS 480

Query: 481 LEKLKWSVLTCYDVSWVWHQPSCCL 506
           LEKLKWSVLTCYDVSWVWHQPSCCL
Sbjct: 481 LEKLKWSVLTCYDVSWVWHQPSCCL 505

BLAST of Carg27333 vs. NCBI nr
Match: KAG6591379.1 (THO complex subunit 5B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 903.3 bits (2333), Expect = 9.6e-259
Identity = 476/485 (98.14%), Postives = 477/485 (98.35%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDESEPPPPDSDSGKMSPYEMLRESK SVEEIVAKMLSIKKQGEPKTQLGELVTQML
Sbjct: 8   MLIEDESEPPPPDSDSGKMSPYEMLRESKSSVEEIVAKMLSIKKQGEPKTQLGELVTQML 67

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 68  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 127

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK
Sbjct: 128 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 187

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 188 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 247

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDA DEDDDGQ
Sbjct: 248 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDAPDEDDDGQ 307

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICV 360
           RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICV
Sbjct: 308 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICV 367

Query: 361 GIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGID 420
           GIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGID
Sbjct: 368 GIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGID 427

Query: 421 FLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDS 480
           FLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALV     
Sbjct: 428 FLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVNIDSP 487

Query: 481 LEKLK 486
           +E LK
Sbjct: 488 VENLK 492

BLAST of Carg27333 vs. NCBI nr
Match: XP_038897136.1 (THO complex subunit 5B [Benincasa hispida])

HSP 1 Score: 888.6 bits (2295), Expect = 2.4e-254
Identity = 463/506 (91.50%), Postives = 483/506 (95.45%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE++PPPPD D+GKMSPYEMLRESK SVEEIV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETDPPPPDGDTGKMSPYEMLRESKSSVEEIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRL +E+FQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLDYEVFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG VKDAQAFARHQAN+ETGASTN E++KLEDDALDEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSVKDAQAFARHQANKETGASTNTESNKLEDDALDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKV+IEHAGIYQVHPLKIILHIY NETC+ KSMKLLS+KFE LLKLN+IC
Sbjct: 309 RRRKRPKKIPAKVDIEHAGIYQVHPLKIILHIYGNETCEPKSMKLLSLKFEWLLKLNVIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVVGET+AFSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVGETIAFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQESA GETVR+DIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESAGGETVRNDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC DV WV H+P CCL
Sbjct: 489 SLEKLKWPVLTCDDVPWVLHKPLCCL 514

BLAST of Carg27333 vs. NCBI nr
Match: XP_022982205.1 (THO complex subunit 5B [Cucurbita maxima])

HSP 1 Score: 877.5 bits (2266), Expect = 5.6e-251
Identity = 460/506 (90.91%), Postives = 480/506 (94.86%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+EPPP +S++GK+SPYEMLRESK SVEEIV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETEPPPQESETGKISPYEMLRESKSSVEEIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPEDIKNS RSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPEDIKNSIRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLG+LQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGMLQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG VKDAQAFARHQAN++ GASTN E+SKLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSVKDAQAFARHQANKDAGASTNVESSKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETC+ KSMKLLS+KFECL KLNIIC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCEPKSMKLLSLKFECLSKLNIIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVV ETL+FSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVRETLSFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V W  H+PSC L
Sbjct: 489 SLEKLKWPVLTCDNVPWASHKPSCSL 514

BLAST of Carg27333 vs. NCBI nr
Match: XP_008452557.1 (PREDICTED: THO complex subunit 5B [Cucumis melo] >KAA0064387.1 THO complex subunit 5B [Cucumis melo var. makuwa])

HSP 1 Score: 875.9 bits (2262), Expect = 1.6e-250
Identity = 456/506 (90.12%), Postives = 480/506 (94.86%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+ P PPDS++GK+SP+EMLRESK  VE+IV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETAPLPPDSETGKISPFEMLRESKSCVEDIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVK+ETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKSETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPE+IKNSTRSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPENIKNSTRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG +KDAQAFARHQAN+ETG+S NAE++KLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSIKDAQAFARHQANKETGSSNNAESNKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNE C+ KSMKLLS+KFECLLKLN+IC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNEICEPKSMKLLSLKFECLLKLNVIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQES SGE VR DIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESVSGEPVRGDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V WV H+PSCCL
Sbjct: 489 SLEKLKWPVLTCDEVPWVSHKPSCCL 514

BLAST of Carg27333 vs. ExPASy Swiss-Prot
Match: F4K4J0 (THO complex subunit 5B OS=Arabidopsis thaliana OX=3702 GN=THO5B PE=1 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 4.0e-175
Identity = 329/496 (66.33%), Postives = 395/496 (79.64%), Query Frame = 0

Query: 12  PDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQMLLHFVTLRQANR 71
           P  + GK SP E+LRESK SVEEIVAKMLS+KKQG  K+++ EL+TQM L+FV LRQANR
Sbjct: 29  PPREPGK-SPLELLRESKTSVEEIVAKMLSMKKQGNHKSEIRELLTQMFLNFVNLRQANR 88

Query: 72  SILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSED 131
           +IL EED+VKAETERAKAPVDFTTLQL+NLMYEKSHYVKAIKAC+DFKSKY D +LV E 
Sbjct: 89  AILTEEDKVKAETERAKAPVDFTTLQLHNLMYEKSHYVKAIKACRDFKSKYPDIDLVPEQ 148

Query: 132 EFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRK 191
           +FFR APE IK+ + S DS+H LM +RL+FEL QRKEL K R  LEQ KKSLLE IA RK
Sbjct: 149 DFFRHAPEAIKDQSLSSDSSHVLMPKRLNFELHQRKELCKHRARLEQQKKSLLETIAERK 208

Query: 192 KFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFN 251
           KF+SSLP HLKSLKKASLPVQN LGI  TKKLKQH LAELLPPPLYV+YSQ LAQKEAF 
Sbjct: 209 KFLSSLPLHLKSLKKASLPVQNHLGIQHTKKLKQHNLAELLPPPLYVLYSQLLAQKEAFE 268

Query: 252 ESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQRRKKRPKKIPA 311
           ESIELE+VG +KDAQA+AR Q+ +++G S+N E+S+LEDD  D+DDDGQRR+KRPKK+ +
Sbjct: 269 ESIELEVVGSLKDAQAYARQQSRKDSGMSSNTESSRLEDDGPDDDDDGQRRRKRPKKLTS 328

Query: 312 KVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIICVGIEGSHEGPE 371
           K   + AG+YQVHPLKI+LHIYD+E  D KS+KL+ +KFE LLKLN++CVG EGS +GPE
Sbjct: 329 KEGSDKAGLYQVHPLKIVLHIYDDEIPDTKSLKLVILKFEYLLKLNVVCVGAEGSQDGPE 388

Query: 372 NNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGIDFLPELPPLVS 431
            NI C+LFPDD GLE PHQS KL++G+   F + RTSRPYKW QHLAGID    + P++ 
Sbjct: 389 KNIFCNLFPDDAGLEPPHQSTKLILGDGQTFDENRTSRPYKWVQHLAGID----ISPVLL 448

Query: 432 AQESASGETVRSD-IVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDSLEKLKWSVL 491
            QE+ + +  +SD  V  LS+YRQQ R+QTV++R+R +KKA LAL EQLD L K +  V+
Sbjct: 449 GQEAHNTDPAKSDTFVPDLSLYRQQHRVQTVLRRIRLRKKAHLALAEQLDLLMKHELPVV 508

Query: 492 TCYDVSWVWHQPSCCL 506
            C D  W  H+  C L
Sbjct: 509 NCEDAPWALHKVLCAL 519

BLAST of Carg27333 vs. ExPASy Swiss-Prot
Match: F4HRC1 (THO complex subunit 5A OS=Arabidopsis thaliana OX=3702 GN=THO5A PE=1 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.3e-138
Identity = 271/461 (58.79%), Postives = 346/461 (75.05%), Query Frame = 0

Query: 16  SGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQMLLHFVTLRQANRSILL 75
           + + SP E+L+++K SVE I+AKMLSIK+QG PK++  EL+TQM L+F+ LRQANRSIL+
Sbjct: 12  TAEKSPLELLQQTKASVEAIIAKMLSIKQQGTPKSENRELLTQMFLNFINLRQANRSILI 71

Query: 76  EEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFR 135
           EE++V+ ETE AK+PVDFTTL+L+NLMYEKSHY+KA KA +DFKS+Y + +L+SE +FF 
Sbjct: 72  EEEKVRTETEIAKSPVDFTTLELHNLMYEKSHYLKANKASRDFKSRYPNIDLISEQDFFS 131

Query: 136 DAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRKKFMS 195
           DAPE IK+ T S DS+H+LML+RL+FEL QRKEL K R  LEQ KKSLLE  A R KF+S
Sbjct: 132 DAPEAIKSQTLSNDSSHDLMLKRLNFELHQRKELCKLRVRLEQQKKSLLESNAERNKFLS 191

Query: 196 SLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIE 255
           SLP HLKSLKKASLPVQ+QL +   KKLK H LAELLPPPLYVIYSQF+A KEAF E+I+
Sbjct: 192 SLPVHLKSLKKASLPVQSQLSLQNQKKLKYHNLAELLPPPLYVIYSQFMALKEAFEENID 251

Query: 256 LEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQRRKKRPKKIPAKVNI 315
           +E+ G +KDAQ +AR QA +      N+E+ +LE        D +R++KR K    KV  
Sbjct: 252 VEVSGSLKDAQTYARQQAEQ------NSESLRLEVGV-----DEERQRKRLK----KVGS 311

Query: 316 EHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIICVGIEGSHEGPENNIL 375
           +  G+YQVHPLK++LH+YD+E  D KS +L+ +KFE LLKLN++CVGIE S +G E NIL
Sbjct: 312 DEGGVYQVHPLKVVLHVYDDEITDPKSHELVMLKFEYLLKLNVVCVGIEESEDGLEKNIL 371

Query: 376 CSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGIDFLPELPPLVSAQES 435
           C+LFPDD+GLE PHQSAKL++G    F   RTSRPYKWAQHLAGI+ LPE+ P  + +  
Sbjct: 372 CNLFPDDSGLEPPHQSAKLILGNDHVFDKSRTSRPYKWAQHLAGIETLPEMSPFFTDK-- 431

Query: 436 ASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALV 476
              +   SD   G +       +QTV+QR+RSQKK +L LV
Sbjct: 432 ---DIQYSDTAKGYASASDHRNVQTVLQRIRSQKKTKLTLV 452

BLAST of Carg27333 vs. ExPASy Swiss-Prot
Match: Q13769 (THO complex subunit 5 homolog OS=Homo sapiens OX=9606 GN=THOC5 PE=1 SV=2)

HSP 1 Score: 166.0 bits (419), Expect = 1.1e-39
Identity = 140/481 (29.11%), Postives = 244/481 (50.73%), Query Frame = 0

Query: 22  YEMLRESKISVEEIVAKMLSIKKQG--EPKTQLGELVTQMLLHFVTLRQANRSILLEEDR 81
           YE+ + +   ++ ++A++  +K +G  +   ++ E   Q  +HF+TL++ NR   +   +
Sbjct: 52  YELYKYTCQELQRLMAEIQDLKSRGGKDVAIEIEERRIQSCVHFMTLKKLNRLAHIRLKK 111

Query: 82  VKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFRDAPE 141
            + +T  AK  VD   LQL NL+YE  H  K I  C +FKSK+ + +LVS +EF+++AP 
Sbjct: 112 GRDQTHEAKQKVDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHEEIDLVSLEEFYKEAPP 171

Query: 142 DIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRKKFMSSLPS 201
           DI  +  +    H   L RL +EL QRK L ++  E   +K+ +L+ I  +K+++SSL  
Sbjct: 172 DISKAEVTMGDPHQQTLARLDWELEQRKRLAEKYRECLSNKEKILKEIEVKKEYLSSLQP 231

Query: 202 HLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIELEIV 261
            L S+ +ASLPVQ  L +   +  KQ++ A  LPPPLYV++ Q  A  +A ++++ + I 
Sbjct: 232 RLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAIE 291

Query: 262 GIVKDAQAFARHQANEETGASTNAENSKLEDDALDED-DDGQRRKKRPKKIPAKVNIEHA 321
           G V +A+A  +   +              +DD  D D ++ Q  K+R   +  +++ +  
Sbjct: 292 GSVDEAKALFKPPEDS-------------QDDESDSDAEEEQTTKRRRPTLGVQLDDKRK 351

Query: 322 GIYQVHPLKIILHIYDNETCDKSMKLLSVKFECLLKLNIICVGIE-------------GS 381
            + + HPL ++L +     C K   +L + F  L+ LNI+ V  +             G 
Sbjct: 352 EMLKRHPLSVMLDL----KC-KDDSVLHLTFYYLMNLNIMTVKAKVTTAMELITPISAGD 411

Query: 382 HEGPENNILCSLFPDDTGLELPHQSAKLVVGET--LAFSD--KRTSRPYKWAQHLAGIDF 441
              P++ + C L+P D G + P+ + +    +   L  SD       PY W Q L G+ F
Sbjct: 412 LLSPDSVLSC-LYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHF 471

Query: 442 LPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDSL 483
             E P           +TV +D     S       ++T ++ L+++ +++LAL +Q  SL
Sbjct: 472 PKEQPQ----------QTVIADHSLSAS------HMETTMKLLKTRVQSRLALHKQFASL 497

BLAST of Carg27333 vs. ExPASy Swiss-Prot
Match: Q5ZJK1 (THO complex subunit 5 homolog OS=Gallus gallus OX=9031 GN=THOC5 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 3.2e-39
Identity = 143/493 (29.01%), Postives = 248/493 (50.30%), Query Frame = 0

Query: 22  YEMLRESKISVEEIVAKMLSIKKQG--EPKTQLGELVTQMLLHFVTLRQANRSILLEEDR 81
           YE+ +E+   ++ ++A++  +K +G  +  +++ E   Q  +HF+TL++ NR   +   +
Sbjct: 51  YELYKETCQELQRLMAEIQELKSRGVKDNASEIDERRVQSCVHFMTLKKLNRLAHIRLKK 110

Query: 82  VKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFRDAPE 141
            + +T  AK  VD   LQL NL+YE  H  K I  C +FKSK+ + ELVS +EF+ +AP 
Sbjct: 111 GRDQTHEAKQKVDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHEEIELVSLEEFYSEAPT 170

Query: 142 DIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRKKFMSSLPS 201
           +I     +    H   L RL +EL QRK L +R  E +  K+ +L+ I  +K+++SSL  
Sbjct: 171 EISRPDITLTEPHQQTLARLDWELEQRKRLAERYKECQTIKEKILKEIEVKKEYLSSLQP 230

Query: 202 HLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIELEIV 261
            L S+ +ASLPVQ  L +   +  KQ++ A  LPPPLYV++ Q  A  +A ++ + + I 
Sbjct: 231 RLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQASAYGQACDKKLVVAIE 290

Query: 262 GIVKDAQAFARHQANEETGASTNAENSKLEDDALDED-DDGQRRKKRPKKIPAKVNIEHA 321
           G V++A+A   ++  E++           +DD  D D ++ Q  K+R   +  +++ +  
Sbjct: 291 GSVEEAKAL--YKPPEDS-----------QDDESDSDMEEEQTTKRRRPTLGVQLDDKRK 350

Query: 322 GIYQVHPLKI----------ILHIYDNETCDKS-MKLLSVKFECLLKLNIICVGIE---- 381
            + + HPL +          +LH+      + + M +L + F  L+ LNI+ V  +    
Sbjct: 351 EMLKRHPLSVTVDLKCKDENVLHLTFYYLMNLNIMTVLHLTFYYLMNLNIMTVKTKVTTA 410

Query: 382 ---------GSHEGPENNILCSLFPDDTGLELPHQSAKLVVGET--LAFSDKRT--SRPY 441
                    G    P++ + C L+P D G + P+ + +    +   L  SD  T    PY
Sbjct: 411 AELTTAISAGDLLSPDSLLSC-LYPGDHGKKTPNPANQFQFDKVGILTLSDYVTELGHPY 470

Query: 442 KWAQHLAGIDFLPELPP-LVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKK 483
            W Q L G+ F  + P   V+A  S S                    ++  ++ LR++ +
Sbjct: 471 VWVQKLGGLHFPKDQPQHTVTADNSLSAS-----------------HMEMTMKLLRTRLQ 512

BLAST of Carg27333 vs. ExPASy Swiss-Prot
Match: Q68FX7 (THO complex subunit 5 homolog OS=Rattus norvegicus OX=10116 GN=Thoc5 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.4e-39
Identity = 132/475 (27.79%), Postives = 239/475 (50.32%), Query Frame = 0

Query: 22  YEMLRESKISVEEIVAKMLSIKKQG--EPKTQLGELVTQMLLHFVTLRQANRSILLEEDR 81
           YE+ + +   ++ ++A++  +K +G  +   ++ E   Q  +HF+TL++ NR   +   +
Sbjct: 52  YELYKYTCQELQRLMAEIQDLKSKGSKDVAIEIEERRIQSCVHFMTLKKLNRLAHIRLKK 111

Query: 82  VKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFRDAPE 141
            + +T  AK  VD   LQL NL+YE  H  K I  C +FKSK+ + +LVS +EF+ +AP 
Sbjct: 112 GRDQTHEAKQKVDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHEEIDLVSLEEFYSEAPP 171

Query: 142 DIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRKKFMSSLPS 201
           +I  +  +    H   L RL +EL QRK L ++  E   +K+ +L+ I  +K+++SSL  
Sbjct: 172 NISKAEITMGDPHQQTLARLDWELEQRKRLAEKYRECLSNKEKILKEIEVKKEYLSSLQP 231

Query: 202 HLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIELEIV 261
            L S+ +ASLPVQ  L +   +  KQ++ A  LPPPLYV++ Q  A  +A ++++ + I 
Sbjct: 232 RLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAIE 291

Query: 262 GIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQRRKKRPKKIPAKVNIEHAG 321
           G V +A+A  +   +              +DD  D D + ++  +R   +  +++ +   
Sbjct: 292 GSVDEAKALFKPPEDS-------------QDDESDSDAEEEQTTRRRPTLGVQLDDKRKE 351

Query: 322 IYQVHPLKIILHI--YDNETCD------KSMKLLSVKFECLLKLNIICVGIEGSHEGPEN 381
           + + HPL ++L +   DN           ++ +++VK +    + +I     G    P++
Sbjct: 352 MLKRHPLSVLLDLKCKDNSVLHLTFFYLMNLNIMTVKAKVTTAMELITPISAGDLLSPDS 411

Query: 382 NILCSLFPDDTGLELPHQSAKLVVGE----TLAFSDKRTSRPYKWAQHLAGIDFLPELPP 441
            + C L+P D G + P+ + +    +    TL         PY W Q L G+ F  E P 
Sbjct: 412 VLSC-LYPGDHGKKTPNPANQYQFDKVGILTLRDYVLELGHPYLWVQKLGGLHFPKEQPQ 471

Query: 442 LVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDSLE 483
                      TV +D     S       ++T ++ L+++ +++LAL +Q  SLE
Sbjct: 472 ----------HTVMADHSQSAS------HMETTMKLLKTRVQSRLALHKQFASLE 496

BLAST of Carg27333 vs. ExPASy TrEMBL
Match: A0A6J1J206 (THO complex subunit 5B OS=Cucurbita maxima OX=3661 GN=LOC111481103 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.7e-251
Identity = 460/506 (90.91%), Postives = 480/506 (94.86%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+EPPP +S++GK+SPYEMLRESK SVEEIV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETEPPPQESETGKISPYEMLRESKSSVEEIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPEDIKNS RSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPEDIKNSIRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLG+LQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGMLQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG VKDAQAFARHQAN++ GASTN E+SKLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSVKDAQAFARHQANKDAGASTNVESSKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETC+ KSMKLLS+KFECL KLNIIC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCEPKSMKLLSLKFECLSKLNIIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVV ETL+FSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVRETLSFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V W  H+PSC L
Sbjct: 489 SLEKLKWPVLTCDNVPWASHKPSCSL 514

BLAST of Carg27333 vs. ExPASy TrEMBL
Match: A0A5A7VBI1 (THO complex subunit 5B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00890 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.9e-251
Identity = 456/506 (90.12%), Postives = 480/506 (94.86%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+ P PPDS++GK+SP+EMLRESK  VE+IV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETAPLPPDSETGKISPFEMLRESKSCVEDIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVK+ETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKSETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPE+IKNSTRSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPENIKNSTRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG +KDAQAFARHQAN+ETG+S NAE++KLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSIKDAQAFARHQANKETGSSNNAESNKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNE C+ KSMKLLS+KFECLLKLN+IC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNEICEPKSMKLLSLKFECLLKLNVIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQES SGE VR DIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESVSGEPVRGDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V WV H+PSCCL
Sbjct: 489 SLEKLKWPVLTCDEVPWVSHKPSCCL 514

BLAST of Carg27333 vs. ExPASy TrEMBL
Match: A0A1S3BTI6 (THO complex subunit 5B OS=Cucumis melo OX=3656 GN=LOC103493544 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.9e-251
Identity = 456/506 (90.12%), Postives = 480/506 (94.86%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+ P PPDS++GK+SP+EMLRESK  VE+IV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETAPLPPDSETGKISPFEMLRESKSCVEDIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVK+ETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKSETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPE+IKNSTRSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPENIKNSTRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG +KDAQAFARHQAN+ETG+S NAE++KLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSIKDAQAFARHQANKETGSSNNAESNKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNE C+ KSMKLLS+KFECLLKLN+IC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNEICEPKSMKLLSLKFECLLKLNVIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQES SGE VR DIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESVSGEPVRGDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V WV H+PSCCL
Sbjct: 489 SLEKLKWPVLTCDEVPWVSHKPSCCL 514

BLAST of Carg27333 vs. ExPASy TrEMBL
Match: A0A0A0KZS2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639830 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.7e-250
Identity = 455/506 (89.92%), Postives = 479/506 (94.66%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+EP PPDS++GK+SP+EMLRESK  VE+IV KMLSIKK GE KTQL ELVTQM 
Sbjct: 9   MLIEDETEPLPPDSETGKISPFEMLRESKSCVEDIVTKMLSIKKHGESKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVK+ETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKSETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFFRDAPE+IKNS RSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFRDAPENIKNSMRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           K LLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KGLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG +KDAQAFARHQAN+ETGAS NAE++KLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSIKDAQAFARHQANKETGASNNAESNKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYD+ETC+ KSMKLLS+KFECLLKLN+IC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDSETCEPKSMKLLSLKFECLLKLNVIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQES SGE VR DIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESVSGEPVRGDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V WV H+PSCCL
Sbjct: 489 SLEKLKWPVLTCDEVPWVSHKPSCCL 514

BLAST of Carg27333 vs. ExPASy TrEMBL
Match: A0A6J1FJR5 (THO complex subunit 5B OS=Cucurbita moschata OX=3662 GN=LOC111445974 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.5e-249
Identity = 457/506 (90.32%), Postives = 478/506 (94.47%), Query Frame = 0

Query: 1   MLIEDESEPPPPDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQML 60
           MLIEDE+EPPP +S++GK+SPYEMLRESK SVEEIV KMLSIKK GEPKTQL ELVTQM 
Sbjct: 9   MLIEDETEPPPQESETGKISPYEMLRESKSSVEEIVTKMLSIKKHGEPKTQLRELVTQMF 68

Query: 61  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 120
           LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS
Sbjct: 69  LHFVTLRQANRSILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKS 128

Query: 121 KYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHK 180
           KY D ELVSEDEFF+DAPEDIK S RSKDSAHNLMLQRL +ELFQRKEL KRRDELEQHK
Sbjct: 129 KYPDIELVSEDEFFQDAPEDIKKSIRSKDSAHNLMLQRLDYELFQRKELCKRRDELEQHK 188

Query: 181 KSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIY 240
           KSLLEVIANRKKF+SSLPSHLKSLKKASLPVQNQLG+LQTKKLKQHQLAELLPPPLYVIY
Sbjct: 189 KSLLEVIANRKKFLSSLPSHLKSLKKASLPVQNQLGMLQTKKLKQHQLAELLPPPLYVIY 248

Query: 241 SQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQ 300
           SQFLAQKEAF E+IELEIVG VKDAQAFARHQAN++ GASTN E+SKLEDDA DEDDDGQ
Sbjct: 249 SQFLAQKEAFGENIELEIVGSVKDAQAFARHQANKDAGASTNVESSKLEDDAPDEDDDGQ 308

Query: 301 RRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIIC 360
           RR+KRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETC+ KSMKLLS+KFECL KLNIIC
Sbjct: 309 RRRKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCEPKSMKLLSLKFECLSKLNIIC 368

Query: 361 VGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGI 420
           VGIEGSHEGPENNILC+LFPDDTGLELPHQ AKLVV ETL+FSDKRTSRPYKWAQHLAGI
Sbjct: 369 VGIEGSHEGPENNILCNLFPDDTGLELPHQPAKLVVRETLSFSDKRTSRPYKWAQHLAGI 428

Query: 421 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLD 480
           DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQ RIQTVVQRLRS+KKAQLALVEQLD
Sbjct: 429 DFLPELPPLVSAQESASGETVRSDIVSGLSMYRQQNRIQTVVQRLRSRKKAQLALVEQLD 488

Query: 481 SLEKLKWSVLTCYDVSWVWHQPSCCL 506
           SLEKLKW VLTC +V W  H+PSC L
Sbjct: 489 SLEKLKWPVLTCDNVPWASHKPSCSL 514

BLAST of Carg27333 vs. TAIR 10
Match: AT5G42920.2 (THO complex, subunit 5 )

HSP 1 Score: 615.9 bits (1587), Expect = 2.8e-176
Identity = 329/496 (66.33%), Postives = 395/496 (79.64%), Query Frame = 0

Query: 12  PDSDSGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQMLLHFVTLRQANR 71
           P  + GK SP E+LRESK SVEEIVAKMLS+KKQG  K+++ EL+TQM L+FV LRQANR
Sbjct: 29  PPREPGK-SPLELLRESKTSVEEIVAKMLSMKKQGNHKSEIRELLTQMFLNFVNLRQANR 88

Query: 72  SILLEEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSED 131
           +IL EED+VKAETERAKAPVDFTTLQL+NLMYEKSHYVKAIKAC+DFKSKY D +LV E 
Sbjct: 89  AILTEEDKVKAETERAKAPVDFTTLQLHNLMYEKSHYVKAIKACRDFKSKYPDIDLVPEQ 148

Query: 132 EFFRDAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRK 191
           +FFR APE IK+ + S DS+H LM +RL+FEL QRKEL K R  LEQ KKSLLE IA RK
Sbjct: 149 DFFRHAPEAIKDQSLSSDSSHVLMPKRLNFELHQRKELCKHRARLEQQKKSLLETIAERK 208

Query: 192 KFMSSLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFN 251
           KF+SSLP HLKSLKKASLPVQN LGI  TKKLKQH LAELLPPPLYV+YSQ LAQKEAF 
Sbjct: 209 KFLSSLPLHLKSLKKASLPVQNHLGIQHTKKLKQHNLAELLPPPLYVLYSQLLAQKEAFE 268

Query: 252 ESIELEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQRRKKRPKKIPA 311
           ESIELE+VG +KDAQA+AR Q+ +++G S+N E+S+LEDD  D+DDDGQRR+KRPKK+ +
Sbjct: 269 ESIELEVVGSLKDAQAYARQQSRKDSGMSSNTESSRLEDDGPDDDDDGQRRRKRPKKLTS 328

Query: 312 KVNIEHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIICVGIEGSHEGPE 371
           K   + AG+YQVHPLKI+LHIYD+E  D KS+KL+ +KFE LLKLN++CVG EGS +GPE
Sbjct: 329 KEGSDKAGLYQVHPLKIVLHIYDDEIPDTKSLKLVILKFEYLLKLNVVCVGAEGSQDGPE 388

Query: 372 NNILCSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGIDFLPELPPLVS 431
            NI C+LFPDD GLE PHQS KL++G+   F + RTSRPYKW QHLAGID    + P++ 
Sbjct: 389 KNIFCNLFPDDAGLEPPHQSTKLILGDGQTFDENRTSRPYKWVQHLAGID----ISPVLL 448

Query: 432 AQESASGETVRSD-IVSGLSMYRQQIRIQTVVQRLRSQKKAQLALVEQLDSLEKLKWSVL 491
            QE+ + +  +SD  V  LS+YRQQ R+QTV++R+R +KKA LAL EQLD L K +  V+
Sbjct: 449 GQEAHNTDPAKSDTFVPDLSLYRQQHRVQTVLRRIRLRKKAHLALAEQLDLLMKHELPVV 508

Query: 492 TCYDVSWVWHQPSCCL 506
            C D  W  H+  C L
Sbjct: 509 NCEDAPWALHKVLCAL 519

BLAST of Carg27333 vs. TAIR 10
Match: AT5G42920.1 (THO complex, subunit 5 )

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-140
Identity = 262/406 (64.53%), Postives = 317/406 (78.08%), Query Frame = 0

Query: 102 MYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFRDAPEDIKNSTRSKDSAHNLMLQRLHF 161
           MYEKSHYVKAIKAC+DFKSKY D +LV E +FFR APE IK+ + S DS+H LM +RL+F
Sbjct: 1   MYEKSHYVKAIKACRDFKSKYPDIDLVPEQDFFRHAPEAIKDQSLSSDSSHVLMPKRLNF 60

Query: 162 ELFQRKELRKRRDELEQHKKSLLEVIANRKKFMSSLPSHLKSLKKASLPVQNQLGILQTK 221
           EL QRKEL K R  LEQ KKSLLE IA RKKF+SSLP HLKSLKKASLPVQN LGI  TK
Sbjct: 61  ELHQRKELCKHRARLEQQKKSLLETIAERKKFLSSLPLHLKSLKKASLPVQNHLGIQHTK 120

Query: 222 KLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIELEIVGIVKDAQAFARHQANEETGAST 281
           KLKQH LAELLPPPLYV+YSQ LAQKEAF ESIELE+VG +KDAQA+AR Q+ +++G S+
Sbjct: 121 KLKQHNLAELLPPPLYVLYSQLLAQKEAFEESIELEVVGSLKDAQAYARQQSRKDSGMSS 180

Query: 282 NAENSKLEDDALDEDDDGQRRKKRPKKIPAKVNIEHAGIYQVHPLKIILHIYDNETCD-K 341
           N E+S+LEDD  D+DDDGQRR+KRPKK+ +K   + AG+YQVHPLKI+LHIYD+E  D K
Sbjct: 181 NTESSRLEDDGPDDDDDGQRRRKRPKKLTSKEGSDKAGLYQVHPLKIVLHIYDDEIPDTK 240

Query: 342 SMKLLSVKFECLLKLNIICVGIEGSHEGPENNILCSLFPDDTGLELPHQSAKLVVGETLA 401
           S+KL+ +KFE LLKLN++CVG EGS +GPE NI C+LFPDD GLE PHQS KL++G+   
Sbjct: 241 SLKLVILKFEYLLKLNVVCVGAEGSQDGPEKNIFCNLFPDDAGLEPPHQSTKLILGDGQT 300

Query: 402 FSDKRTSRPYKWAQHLAGIDFLPELPPLVSAQESASGETVRSD-IVSGLSMYRQQIRIQT 461
           F + RTSRPYKW QHLAGID    + P++  QE+ + +  +SD  V  LS+YRQQ R+QT
Sbjct: 301 FDENRTSRPYKWVQHLAGID----ISPVLLGQEAHNTDPAKSDTFVPDLSLYRQQHRVQT 360

Query: 462 VVQRLRSQKKAQLALVEQLDSLEKLKWSVLTCYDVSWVWHQPSCCL 506
           V++R+R +KKA LAL EQLD L K +  V+ C D  W  H+  C L
Sbjct: 361 VLRRIRLRKKAHLALAEQLDLLMKHELPVVNCEDAPWALHKVLCAL 402

BLAST of Carg27333 vs. TAIR 10
Match: AT1G45233.2 (THO complex, subunit 5 )

HSP 1 Score: 494.6 bits (1272), Expect = 9.6e-140
Identity = 271/461 (58.79%), Postives = 346/461 (75.05%), Query Frame = 0

Query: 16  SGKMSPYEMLRESKISVEEIVAKMLSIKKQGEPKTQLGELVTQMLLHFVTLRQANRSILL 75
           + + SP E+L+++K SVE I+AKMLSIK+QG PK++  EL+TQM L+F+ LRQANRSIL+
Sbjct: 12  TAEKSPLELLQQTKASVEAIIAKMLSIKQQGTPKSENRELLTQMFLNFINLRQANRSILI 71

Query: 76  EEDRVKAETERAKAPVDFTTLQLNNLMYEKSHYVKAIKACKDFKSKYSDSELVSEDEFFR 135
           EE++V+ ETE AK+PVDFTTL+L+NLMYEKSHY+KA KA +DFKS+Y + +L+SE +FF 
Sbjct: 72  EEEKVRTETEIAKSPVDFTTLELHNLMYEKSHYLKANKASRDFKSRYPNIDLISEQDFFS 131

Query: 136 DAPEDIKNSTRSKDSAHNLMLQRLHFELFQRKELRKRRDELEQHKKSLLEVIANRKKFMS 195
           DAPE IK+ T S DS+H+LML+RL+FEL QRKEL K R  LEQ KKSLLE  A R KF+S
Sbjct: 132 DAPEAIKSQTLSNDSSHDLMLKRLNFELHQRKELCKLRVRLEQQKKSLLESNAERNKFLS 191

Query: 196 SLPSHLKSLKKASLPVQNQLGILQTKKLKQHQLAELLPPPLYVIYSQFLAQKEAFNESIE 255
           SLP HLKSLKKASLPVQ+QL +   KKLK H LAELLPPPLYVIYSQF+A KEAF E+I+
Sbjct: 192 SLPVHLKSLKKASLPVQSQLSLQNQKKLKYHNLAELLPPPLYVIYSQFMALKEAFEENID 251

Query: 256 LEIVGIVKDAQAFARHQANEETGASTNAENSKLEDDALDEDDDGQRRKKRPKKIPAKVNI 315
           +E+ G +KDAQ +AR QA +      N+E+ +LE        D +R++KR K    KV  
Sbjct: 252 VEVSGSLKDAQTYARQQAEQ------NSESLRLEVGV-----DEERQRKRLK----KVGS 311

Query: 316 EHAGIYQVHPLKIILHIYDNETCD-KSMKLLSVKFECLLKLNIICVGIEGSHEGPENNIL 375
           +  G+YQVHPLK++LH+YD+E  D KS +L+ +KFE LLKLN++CVGIE S +G E NIL
Sbjct: 312 DEGGVYQVHPLKVVLHVYDDEITDPKSHELVMLKFEYLLKLNVVCVGIEESEDGLEKNIL 371

Query: 376 CSLFPDDTGLELPHQSAKLVVGETLAFSDKRTSRPYKWAQHLAGIDFLPELPPLVSAQES 435
           C+LFPDD+GLE PHQSAKL++G    F   RTSRPYKWAQHLAGI+ LPE+ P  + +  
Sbjct: 372 CNLFPDDSGLEPPHQSAKLILGNDHVFDKSRTSRPYKWAQHLAGIETLPEMSPFFTDK-- 431

Query: 436 ASGETVRSDIVSGLSMYRQQIRIQTVVQRLRSQKKAQLALV 476
              +   SD   G +       +QTV+QR+RSQKK +L LV
Sbjct: 432 ---DIQYSDTAKGYASASDHRNVQTVLQRIRSQKKTKLTLV 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024259.17.5e-280100.00THO complex subunit 5B [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6591379.19.6e-25998.14THO complex subunit 5B, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038897136.12.4e-25491.50THO complex subunit 5B [Benincasa hispida][more]
XP_022982205.15.6e-25190.91THO complex subunit 5B [Cucurbita maxima][more]
XP_008452557.11.6e-25090.12PREDICTED: THO complex subunit 5B [Cucumis melo] >KAA0064387.1 THO complex subun... [more]
Match NameE-valueIdentityDescription
F4K4J04.0e-17566.33THO complex subunit 5B OS=Arabidopsis thaliana OX=3702 GN=THO5B PE=1 SV=1[more]
F4HRC11.3e-13858.79THO complex subunit 5A OS=Arabidopsis thaliana OX=3702 GN=THO5A PE=1 SV=1[more]
Q137691.1e-3929.11THO complex subunit 5 homolog OS=Homo sapiens OX=9606 GN=THOC5 PE=1 SV=2[more]
Q5ZJK13.2e-3929.01THO complex subunit 5 homolog OS=Gallus gallus OX=9031 GN=THOC5 PE=2 SV=1[more]
Q68FX75.4e-3927.79THO complex subunit 5 homolog OS=Rattus norvegicus OX=10116 GN=Thoc5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J2062.7e-25190.91THO complex subunit 5B OS=Cucurbita maxima OX=3661 GN=LOC111481103 PE=3 SV=1[more]
A0A5A7VBI17.9e-25190.12THO complex subunit 5B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2... [more]
A0A1S3BTI67.9e-25190.12THO complex subunit 5B OS=Cucumis melo OX=3656 GN=LOC103493544 PE=3 SV=1[more]
A0A0A0KZS26.7e-25089.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639830 PE=3 SV=1[more]
A0A6J1FJR51.5e-24990.32THO complex subunit 5B OS=Cucurbita moschata OX=3662 GN=LOC111445974 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G42920.22.8e-17666.33THO complex, subunit 5 [more]
AT5G42920.11.5e-14064.53THO complex, subunit 5 [more]
AT1G45233.29.6e-14058.79THO complex, subunit 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 166..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 272..307
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
IPR019163THO complex, subunit 5PFAMPF09766FmiP_Thoc5coord: 65..421
e-value: 4.0E-102
score: 342.2
IPR019163THO complex, subunit 5PANTHERPTHR13375FMS INTERACTING PROTEINcoord: 13..492

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27333-RACarg27333-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006406 mRNA export from nucleus
biological_process GO:0032786 positive regulation of DNA-templated transcription, elongation
cellular_component GO:0000445 THO complex part of transcription export complex
molecular_function GO:0003729 mRNA binding