Carg27111 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27111
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerase II subunit RPB2
Location	Carg_Chr19: 4199406 .. 4200624 (-)
RNA-Seq Expression	Carg27111
Synteny	Carg27111

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGACGTAACTTTATATGGGCCTAAATGGTTAAAGCCCAAACAGATCGACCTCTATCTGGGCCTAAACTGGTGGAAGCCCAAAATTGAAAGAACCTCTCTCTTTCTTCTCTCCCTCCTCCCGGTGTCAATCGGTGGAGGAATTTCCTGAGAAATTGAAATGGAGAAATCCTTCACTCTGATTCAGACAATTGCGACCGCTGGAGCCTTCTCTGCGATTTCGTTCTGGTGCGATTTTACTTCTCTCTCTTCAACCTTGATGCCTCATTTCTTCTCTGGATCTCTTAGCTCAATTCGTCTTGCTTCATTTTTCTTAGGTATGGATTCATGTTTGGAAGAGAGTCCGCTCGAAAAGACTTGGGAGATTTGATTCAAGATCTACGCCGCGGAAGCTCCAACGATTCTTCTTCTGCAACTCCTCAGTAGGTAATGGTTTTTATACGATGATGTAATTGACGTTCGAGAACTATTAATCGCCTGCTTGTTACTGATCGATTCGATTGTGTTGGAGAAGTTCATTCTGGACTGAATGACAAAATTTAACTTATCGAACAAGGAGATTCGTAATTTTTGATTTGAGGCGTTTGTGAAATGTGGATTAACCTTAAAGCAACGTCGATTTTGAGGCATTTGAAAGAAAAACGTGTTTCTCCATGTGTTCATACACAGATCATTTTGAAGTATGGCGGTGACGAAAGAGGCAGCGTTGCAACTTCGGGGCCATGCGGATCGAGTGCTTGTCAAGTTAAGGATTTTCTGCCAAAAGCTTGGATCAAAGACTCGAAAATTTTCCTTCAATTTGGCGATGTTTCTGTAGAAACGACTTCTTACAAATGCCTCGAAGTTCAGCCGGTCAATTGTTTTCTACGAGTTTCACGTATGGCATTACGTTTTTGAATGATATTCCTTTACTTTCTAGAAATTTTCCTTCGGAACTTGTAGATGTCTGGTCCATTTATTTGCCGATATCGATCATTCAGTTCATCTCAAATATTTTTGGCACAACCAGTAGTGTAAACAGGACTGGCGGCGATTTCCTTTGAAACGGAATTGATGATCCATGTCGTGATGATATCGTTGCGCTGCATTGCCAGGCGGAAGAAAGATTTCCCTTCAAAATATTGATAAGAATGTCTAAACATCCCAATCAAAAGTTTAGAGTGCGTCCCATGAGTGATTTGAAATATAAAATTTTAAGACAAAATATTTTCAAAATTTCAAA

mRNA sequence

TGACGTAACTTTATATGGGCCTAAATGGTTAAAGCCCAAACAGATCGACCTCTATCTGGGCCTAAACTGGTGGAAGCCCAAAATTGAAAGAACCTCTCTCTTTCTTCTCTCCCTCCTCCCGGTGTCAATCGGTGGAGGAATTTCCTGAGAAATTGAAATGGAGAAATCCTTCACTCTGATTCAGACAATTGCGACCGCTGGAGCCTTCTCTGCGATTTCGTTCTGGTATGGATTCATGTTTGGAAGAGAGTCCGCTCGAAAAGACTTGGGAGATTTGATTCAAGATCTACGCCGCGGAAGCTCCAACGATTCTTCTTCTGCAACTCCTCAGTAGATCATTTTGAAGTATGGCGGTGACGAAAGAGGCAGCGTTGCAACTTCGGGGCCATGCGGATCGAGTGCTTGTCAAGTTAAGGATTTTCTGCCAAAAGCTTGGATCAAAGACTCGAAAATTTTCCTTCAATTTGGCGATGTTTCTGTAGAAACGACTTCTTACAAATGCCTCGAAGTTCAGCCGGTCAATTGTTTTCTACGAGTTTCACGTATGGCATTACGTTTTTGAATGATATTCCTTTACTTTCTAGAAATTTTCCTTCGGAACTTGTAGATGTCTGGTCCATTTATTTGCCGATATCGATCATTCAGTTCATCTCAAATATTTTTGGCACAACCAGTAGTGTAAACAGGACTGGCGGCGATTTCCTTTGAAACGGAATTGATGATCCATGTCGTGATGATATCGTTGCGCTGCATTGCCAGGCGGAAGAAAGATTTCCCTTCAAAATATTGATAAGAATGTCTAAACATCCCAATCAAAAGTTTAGAGTGCGTCCCATGAGTGATTTGAAATATAAAATTTTAAGACAAAATATTTTCAAAATTTCAAA

Coding sequence (CDS)

ATGGAGAAATCCTTCACTCTGATTCAGACAATTGCGACCGCTGGAGCCTTCTCTGCGATTTCGTTCTGGTATGGATTCATGTTTGGAAGAGAGTCCGCTCGAAAAGACTTGGGAGATTTGATTCAAGATCTACGCCGCGGAAGCTCCAACGATTCTTCTTCTGCAACTCCTCAGTAG

Protein sequence

MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ

Homology

[Close]

BLAST of Carg27111 vs. NCBI nr
Match: XP_022973256.1 (uncharacterized protein LOC111471813 [Cucurbita maxima] >KAG7011478.1 hypothetical protein SDJN02_26384 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-22
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ 59
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ 58

[Close]

BLAST of Carg27111 vs. NCBI nr
Match: XP_022931780.1 (uncharacterized protein LOC111438056 [Cucurbita moschata])

HSP 1 Score: 113.2 bits (282), Expect = 7.4e-22
Identity = 57/58 (98.28%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ 59
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSA PQ
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSANPQ 58

[Close]

BLAST of Carg27111 vs. NCBI nr
Match: XP_023553989.1 (uncharacterized protein LOC111811404 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108.6 bits (270), Expect = 1.8e-20
Identity = 57/59 (96.61%), Postives = 58/59 (98.31%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSS-NDSSSATPQ 59
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSS +DSSSATPQ
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSDSDSSSATPQ 59

[Close]

BLAST of Carg27111 vs. NCBI nr
Match: XP_022156252.1 (uncharacterized protein LOC111023182 [Momordica charantia] >XP_022156260.1 uncharacterized protein LOC111023182 [Momordica charantia])

HSP 1 Score: 102.8 bits (255), Expect = 1.0e-18
Identity = 52/54 (96.30%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSS 55
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSN  SS
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNSDSS 54

[Close]

BLAST of Carg27111 vs. NCBI nr
Match: XP_016903560.1 (PREDICTED: uncharacterized protein LOC103503490 [Cucumis melo] >KAA0038521.1 putative membrane lipoprotein [Cucumis melo var. makuwa] >TYK31115.1 putative membrane lipoprotein [Cucumis melo var. makuwa])

HSP 1 Score: 102.4 bits (254), Expect = 1.3e-18
Identity = 54/59 (91.53%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRG-SSNDSSSATPQ 59
          M KSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRG S +DSSS TPQ
Sbjct: 1  MGKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGVSKSDSSSETPQ 59

[Close]

BLAST of Carg27111 vs. ExPASy TrEMBL
Match: A0A6J1I859 (uncharacterized protein LOC111471813 OS=Cucurbita maxima OX=3661 GN=LOC111471813 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 9.4e-23
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ 59
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ 58

[Close]

BLAST of Carg27111 vs. ExPASy TrEMBL
Match: A0A6J1F0E3 (uncharacterized protein LOC111438056 OS=Cucurbita moschata OX=3662 GN=LOC111438056 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.6e-22
Identity = 57/58 (98.28%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSATPQ 59
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSA PQ
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSSANPQ 58

[Close]

BLAST of Carg27111 vs. ExPASy TrEMBL
Match: A0A6J1DRK3 (uncharacterized protein LOC111023182 OS=Momordica charantia OX=3673 GN=LOC111023182 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.8e-19
Identity = 52/54 (96.30%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSS 55
          MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSN  SS
Sbjct: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNSDSS 54

[Close]

BLAST of Carg27111 vs. ExPASy TrEMBL
Match: A0A5A7TAA3 (Putative membrane lipoprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003920 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.3e-19
Identity = 54/59 (91.53%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRG-SSNDSSSATPQ 59
          M KSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRG S +DSSS TPQ
Sbjct: 1  MGKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGVSKSDSSSETPQ 59

[Close]

BLAST of Carg27111 vs. ExPASy TrEMBL
Match: A0A1S4E6G4 (uncharacterized protein LOC103503490 OS=Cucumis melo OX=3656 GN=LOC103503490 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.3e-19
Identity = 54/59 (91.53%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRG-SSNDSSSATPQ 59
          M KSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRG S +DSSS TPQ
Sbjct: 1  MGKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGVSKSDSSSETPQ 59

[Close]

BLAST of Carg27111 vs. TAIR 10
Match: AT4G17085.1 (Putative membrane lipoprotein )

HSP 1 Score: 81.6 bits (200), Expect = 2.2e-16
Identity = 38/54 (70.37%), Postives = 47/54 (87.04%), Query Frame = 0


Query: 1  MEKSFTLIQTIATAGAFSAISFWYGFMFGRESARKDLGDLIQDLRRGSSNDSSS 55
          M+KSF+LIQT+A +G FSA+S WYGFMFGRESARK+LG LI++LRRG SN  S+
Sbjct: 1  MQKSFSLIQTVAISGVFSAVSCWYGFMFGRESARKELGGLIEELRRGGSNSDST 54

The following BLAST results are available for this feature:

BLAST of Carg27111 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022973256.1	1.9e-22	100.00	uncharacterized protein LOC111471813 [Cucurbita maxima] >KAG7011478.1 hypothetic...	[more]
XP_022931780.1	7.4e-22	98.28	uncharacterized protein LOC111438056 [Cucurbita moschata]	[more]
XP_023553989.1	1.8e-20	96.61	uncharacterized protein LOC111811404 [Cucurbita pepo subsp. pepo]	[more]
XP_022156252.1	1.0e-18	96.30	uncharacterized protein LOC111023182 [Momordica charantia] >XP_022156260.1 uncha...	[more]
XP_016903560.1	1.3e-18	91.53	PREDICTED: uncharacterized protein LOC103503490 [Cucumis melo] >KAA0038521.1 put...	[more]

BLAST of Carg27111 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg27111 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I859	9.4e-23	100.00	uncharacterized protein LOC111471813 OS=Cucurbita maxima OX=3661 GN=LOC111471813...	[more]
A0A6J1F0E3	3.6e-22	98.28	uncharacterized protein LOC111438056 OS=Cucurbita moschata OX=3662 GN=LOC1114380...	[more]
A0A6J1DRK3	4.8e-19	96.30	uncharacterized protein LOC111023182 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A5A7TAA3	6.3e-19	91.53	Putative membrane lipoprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S4E6G4	6.3e-19	91.53	uncharacterized protein LOC103503490 OS=Cucumis melo OX=3656 GN=LOC103503490 PE=...	[more]

BLAST of Carg27111 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G17085.1	2.2e-16	70.37	Putative membrane lipoprotein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR38384	MEMBRANE LIPOPROTEIN-RELATED	coord: 1..57
None	No IPR available	PANTHER	PTHR38384:SF2	MEMBRANE LIPOPROTEIN-RELATED	coord: 1..57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27111-RA	Carg27111-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032774	RNA biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity