Carg25194 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25194
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription factor Pur-alpha 1-like
LocationCarg_Chr11: 3522450 .. 3526342 (-)
RNA-Seq ExpressionCarg25194
SyntenyCarg25194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGGAGTTCCGGCGGAGGTGGAGTGAGTATTGGGGGAACGACGGCGGGCAGCGTGGCGGTGGGTGGAGTGACGGGTGGCGGCGGCGGAGGAGGAAACGACGTGGAGTTGATGTGCAAAACATTGCAGGTGGAGCACAAATTGTTCTACTTCGATCTGAAGGAGAATCCCAGAGGCCGATATCTGAAGATTTCAGAGAAGACGTCGGCTACAAGGTCAACGATCATTGTTCCCTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTCGACACCAAGGCACCTTTGTTCCTCTATCTCTTTTCTCTGTTCATCGTCTTCCTCCACTTCTACTTTTTAGTTCATCTCTGTGATCTCCTGTTCAACTTTTTGCTAATTGTACTCATCCTGCCTTTCAGGTGTTCTACTTTGACATTGGTGAAAATCGTAGGGGTCGATTCTTGAAGGTAAATTCCGTTTCCTGCTACCATATCTGTTGTTTTTCTTCTCGAGATTCCAACTTTCTTTAATTTCCCACTTTTGTTTTGTTCTTCAAAGTACTCTCTCTAGCTTTTCTGTTTGAATCTGTCAAATTAGGCCTTGTTGATCTAGAATTTCGCTTAAAATTTGCAACAGTTCTTCAGAAAAAGCTGACAGATCCACATTCTTCGAATGATTCTGTACTTTTCAAATTTTTCTTTGATTGAAGTTCTACTTCAACCCAGTTCGTTTCAGTTTACAGATTCTTTATTGATAGCTTCTGATGCTCAAGAACAATATGAACTCTTATCAAAATGGAAGAATGAAATCAATTGAAATATGGGTTTGTAAATGCAGTCATATCCTTGATCTGTGTCCTTTTTAGGAAAAGATAAAAGAAACAAATTCCGTCTTTATGTTCTTGATGACCCTTTATAGCCTTCCATTTGACAATACTTCCAAATGCGTTTTTCCAAATATGAGCAGTTGCCTAAAATTATGCTCTTATTAATGGAGATTCCGAACTGTTTGATTTGACCTTTTTTTTGTTCTTTCTTTCTTTTTAAAGTATTTTTCATTCACCTGTTTCATTGTCCATAGTAATGACAAACCAATGGCTACTTGCAAAATATTCAAGTTTATAAAAGTCTAGGAAACCAAAAGCATTAATATGGCACATTACTATTCAAATCATTAGCATGAACATGTGAAAGACATGGGATATTATAGTATATAATGATTTTCGTAGTTTTACTAGATTTCGATCATGGCAATGTTTAATTTTTCTCATTTTTATAATTTTTTGTATTATGCATCATATGCTTCCACTAAAGCCAACTCTGTGCCATGAATTGCATTATAAATTATTCACACAGTCACGTGTGCAATTTGATTTAATTTCGTTTTGAACCGTGGCTCGTAAATCGTGTCTACACCTTGGTTTACTTAGTTTCAGACAGAATGATGCACATCAGCCATTAGGGTAGTCTGGAGTGAAGTGGCTAGACACTGAAACAAAAGACATGGCTGAACTTGTTGGCTTTAGTTATCAAAAACATTTTCCAATTCTGAGATCAAACCTTGAAACTGGATGTGTTGCTTAAGTTAGGCCAAGGCTTGTATGTAGTGTTGGAAGTATTTTTCGACAATTTCATTGATGATTGAAATTTACAAAAGGGATGTATTATCCATGGTGTTTATAAAAGACCTTCCCAATTTACCGTGAGGATAACTGTAGGATATAGTTTGGTAAAAACAACATTGTCGAAAAATTTTGTGTAGGTCTGTGTCTTTTCTGCAAATATTCTCTGAGTGTTTTCTATCACCAAGAAGTGTTTTCCATCGTCTCAAAACAAAAACATGTTTCTGGTCAAGAAAAATGCATGTTCTAGCTGTTCTCGGTGTGCTCGTGATAGATATTCTCCAGGAATCTATAAGGAGAGAAGAGAGTGCTGATGTAGAATAGCTCATACCCTTCACGTCTGCAGTAACCAGCCTTTTAAAAGTTCTATCTTTTCTATCTCCGTCTTTGGCCATCCATTACCACCTTCTCCATTACCAAGGAGTTGGACCATAATCTCCCTACAGGCTGCCATTGTGCGGCAGCAGATTACAACCACCTCAGGCTCCATAGCCCCTTCATACCAGACAGTTCAGCAACCCCTCTTTTGATCCCTTTGGAGTGTCATCTTCACGTGTCAGTTCACGTAAGTCGAATTTCCAGTTCTCTTATTCCCAGTCTCTCATGCTCTGGCCCTTGTAACATTGCTTGTTGAATCTACAATAAGCTTCCACGTGCGCTACAGATCTCCAATACTCCCACGTTTCTACTTTAGGGTGTACTTTTGTTTGAAGTTTTTTAAACATAGACGTGAGTATGTCGTGTAGTTTTACGCGTGTAAACGAACTTTCTATATCTTATAATATCAGTCTGGATCTGAACTTTGGCTGATTTCAGCTCTCATTTATTCTTATTTTGATCATTTTCGGTTCCGTTACAAATTCGAATTAATGCTGAATGATTCAAATTTTGGGATTAGAGGGTTGATAGTTTGACCTCTTCAGCTATAGACTACTGAGTTCACTGTTGCCTTCTTCTGGATTGTGTGGCTTGAGATTAGGATTTTGGGGAATTGGTGCACGCATCTACTTTATTAATTCTTTTTCTTTGATAAACATTGATGTTTTGTTGGAATTCTTCTCCTTTTTTTTTTTTGGCGCCTCAAAGAAAAGAAAAAAAGATTTTTAAGATCTGGAATGTGTGGTTGTAAATAGAGCTGGTTTTCGTTGTGTTAACTGGGCAGGTATCTGAAGCTTCTGTCAGTAGAAACCGCAGCACCATTATCGTTCCTGCTGGAAGCAATCGGGACGAGGGATGGACTGCGTTCAGAAACATTTTGGCAGAGATCAATGAAGCGTCTAGGCTTTTCATACTGCCCAATCAGGTTTTCTGCTGCTCGTGGTCGTGGCGAGCTTCTCATTATGCCTTTTCACGGGATTACTTCCATTGTTTCTCTTCTAACACAATTTCATACTGTTTTCTTTTGCCCAAACTAGGAAAATTCCGAACATTCAGAACGTCTAGTCGGACTTTCGGATGATGTAGGTGCTGGCTTCATATCAGGTCATAGTAGTCAACCTGGTCCAACCTCTGACTTGAATGTAGACAGACAAGTAGACTTATCAGCTCAAGATGAAATGGGCAATCTGGGGGTATCAAAAGTTATCCGAGCTGATCAGAAGCGATTCTTTTTCGATCTTGGAAGTAACAACCGGGGTCATTTTTTGAGGATATCTGAGGTAATTATACAAAAAACTGAGCTTAAGATCCTTATTTGATCTGGTTTTGTCTGTCTTGAACCCCATGGTGCATTTCTTCAGTGTCTTGATATGAAACTCGTCATATCCGTATCGGTTGCAGGTTGCAGGGGCAGATCGTTCTTCGATCATTCTCCCATTGTCGGGGCTAAAGCAGTTCTATGAAATAGTTGGACATTTCGTGGAGATCACCAAAGATAGGATTGAAGGAATGACGGGCGTGAATGTTAGGACGGTGGATCCGCCTCATAGATGAACACTATAGGCCCAGGGGGGTTTGTGGTGAATAATTTCGGTTAATGTTTGTGTAGATGATGTTCGAGTAGGGTTGAAATTGTGGTGGGTGTGGTTTGATATTGTGTCGTCGTCGTCGTCTTGATTTTCTAGTTCTAAGTCTGAATAATCCATGCTGCTTGATCAGCAGAGGCTATGGAAAATGCTTGAGAAGCTTAATTATTCTTCTTCCATGAATAACACAAGAGAGGGGAAAGAAAGAAAGAAAGAAAGAAAGAAACGATGCTTGTAATTGTCACTTTATAATAATGGTGTGTTCAGGTGTGTTT

mRNA sequence

ATGGAGGGGAGTTCCGGCGGAGGTGGAGTGAGTATTGGGGGAACGACGGCGGGCAGCGTGGCGGTGGGTGGAGTGACGGGTGGCGGCGGCGGAGGAGGAAACGACGTGGAGTTGATGTGCAAAACATTGCAGGTGGAGCACAAATTGTTCTACTTCGATCTGAAGGAGAATCCCAGAGGCCGATATCTGAAGATTTCAGAGAAGACGTCGGCTACAAGGTCAACGATCATTGTTCCCTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTCGACACCAAGGCACCTTTGTTCCTCTATCTCTTTTCTCTGTTCATCGTGTTCTACTTTGACATTGGTGAAAATCGTAGGGGTCGATTCTTGAAGGTATCTGAAGCTTCTGTCAGTAGAAACCGCAGCACCATTATCGTTCCTGCTGGAAGCAATCGGGACGAGGGATGGACTGCGTTCAGAAACATTTTGGCAGAGATCAATGAAGCGTCTAGGCTTTTCATACTGCCCAATCAGGAAAATTCCGAACATTCAGAACGTCTAGTCGGACTTTCGGATGATGTAGGTGCTGGCTTCATATCAGGTCATAGTAGTCAACCTGGTCCAACCTCTGACTTGAATGTAGACAGACAAGTAGACTTATCAGCTCAAGATGAAATGGGCAATCTGGGGGTATCAAAAGTTATCCGAGCTGATCAGAAGCGATTCTTTTTCGATCTTGGAAGTAACAACCGGGGTCATTTTTTGAGGATATCTGAGGTTGCAGGGGCAGATCGTTCTTCGATCATTCTCCCATTGTCGGGGCTAAAGCAGTTCTATGAAATAGTTGGACATTTCGTGGAGATCACCAAAGATAGGATTGAAGGAATGACGGGCGTGAATGTTAGGACGGTGGATCCGCCTCATAGATGAACACTATAGGCCCAGGGGGGTTTGTGGTGAATAATTTCGGTTAATGTTTGTGTAGATGATGTTCGAGTAGGGTTGAAATTGTGGTGGGTGTGGTTTGATATTGTGTCGTCGTCGTCGTCTTGATTTTCTAGTTCTAAGTCTGAATAATCCATGCTGCTTGATCAGCAGAGGCTATGGAAAATGCTTGAGAAGCTTAATTATTCTTCTTCCATGAATAACACAAGAGAGGGGAAAGAAAGAAAGAAAGAAAGAAAGAAACGATGCTTGTAATTGTCACTTTATAATAATGGTGTGTTCAGGTGTGTTT

Coding sequence (CDS)

ATGGAGGGGAGTTCCGGCGGAGGTGGAGTGAGTATTGGGGGAACGACGGCGGGCAGCGTGGCGGTGGGTGGAGTGACGGGTGGCGGCGGCGGAGGAGGAAACGACGTGGAGTTGATGTGCAAAACATTGCAGGTGGAGCACAAATTGTTCTACTTCGATCTGAAGGAGAATCCCAGAGGCCGATATCTGAAGATTTCAGAGAAGACGTCGGCTACAAGGTCAACGATCATTGTTCCCTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTCGACACCAAGGCACCTTTGTTCCTCTATCTCTTTTCTCTGTTCATCGTGTTCTACTTTGACATTGGTGAAAATCGTAGGGGTCGATTCTTGAAGGTATCTGAAGCTTCTGTCAGTAGAAACCGCAGCACCATTATCGTTCCTGCTGGAAGCAATCGGGACGAGGGATGGACTGCGTTCAGAAACATTTTGGCAGAGATCAATGAAGCGTCTAGGCTTTTCATACTGCCCAATCAGGAAAATTCCGAACATTCAGAACGTCTAGTCGGACTTTCGGATGATGTAGGTGCTGGCTTCATATCAGGTCATAGTAGTCAACCTGGTCCAACCTCTGACTTGAATGTAGACAGACAAGTAGACTTATCAGCTCAAGATGAAATGGGCAATCTGGGGGTATCAAAAGTTATCCGAGCTGATCAGAAGCGATTCTTTTTCGATCTTGGAAGTAACAACCGGGGTCATTTTTTGAGGATATCTGAGGTTGCAGGGGCAGATCGTTCTTCGATCATTCTCCCATTGTCGGGGCTAAAGCAGTTCTATGAAATAGTTGGACATTTCGTGGAGATCACCAAAGATAGGATTGAAGGAATGACGGGCGTGAATGTTAGGACGGTGGATCCGCCTCATAGATGA

Protein sequence

MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFSLFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPHR
Homology
BLAST of Carg25194 vs. NCBI nr
Match: KAG7022000.1 (Transcription factor Pur-alpha 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.2 bits (1611), Expect = 3.2e-175
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
           LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL
Sbjct: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS
Sbjct: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR
Sbjct: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTGVNVRTVDPPHR
Sbjct: 301 IEGMTGVNVRTVDPPHR 317

BLAST of Carg25194 vs. NCBI nr
Match: XP_022929520.1 (transcription factor Pur-alpha 1-like [Cucurbita moschata])

HSP 1 Score: 594.0 bits (1530), Expect = 7.8e-166
Identity = 305/317 (96.21%), Postives = 305/317 (96.21%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK         
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS
Sbjct: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR
Sbjct: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTGVNVRTVDPPHR
Sbjct: 301 IEGMTGVNVRTVDPPHR 305

BLAST of Carg25194 vs. NCBI nr
Match: XP_023531642.1 (transcription factor Pur-alpha 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 304/317 (95.90%), Postives = 305/317 (96.21%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEG+SGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK         
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS
Sbjct: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR
Sbjct: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTGVNVRTVDPPHR
Sbjct: 301 IEGMTGVNVRTVDPPHR 305

BLAST of Carg25194 vs. NCBI nr
Match: KAG6588115.1 (Transcription factor Pur-alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.4 bits (1526), Expect = 2.3e-165
Identity = 304/317 (95.90%), Postives = 305/317 (96.21%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK         
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRL
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS
Sbjct: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR
Sbjct: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTGVNVRTVDPPHR
Sbjct: 301 IEGMTGVNVRTVDPPHR 305

BLAST of Carg25194 vs. NCBI nr
Match: XP_022967890.1 (transcription factor Pur-alpha 1-like [Cucurbita maxima])

HSP 1 Score: 587.8 bits (1514), Expect = 5.6e-164
Identity = 304/319 (95.30%), Postives = 305/319 (95.61%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVT--GGGGGGGNDVELMCKTLQVEHKLFYFDLKENP 60
           MEG+SGGGGVSIGGTTAGSVAVGGVT  GGGGGGGNDVELMCKTLQVEHKLFYFDLKENP
Sbjct: 1   MEGNSGGGGVSIGGTTAGSVAVGGVTGGGGGGGGGNDVELMCKTLQVEHKLFYFDLKENP 60

Query: 61  RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYL 120
           RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK       
Sbjct: 61  RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK------- 120

Query: 121 FSLFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEAS 180
                VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEAS
Sbjct: 121 -----VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEAS 180

Query: 181 RLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLG 240
           RLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLG
Sbjct: 181 RLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLG 240

Query: 241 VSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITK 300
           VSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITK
Sbjct: 241 VSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITK 300

Query: 301 DRIEGMTGVNVRTVDPPHR 318
           DRIEGMTGVNVRTVDPPHR
Sbjct: 301 DRIEGMTGVNVRTVDPPHR 307

BLAST of Carg25194 vs. ExPASy Swiss-Prot
Match: Q9SKZ1 (Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 3.4e-124
Identity = 239/318 (75.16%), Postives = 264/318 (83.02%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           ME +SGGGG + GG      AV G  GGGGGGG+DVEL+ KTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEANSGGGGGAEGGR-----AVTG--GGGGGGGSDVELVSKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+K         
Sbjct: 61  RYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGS+ DEGW AFRNILAEI+EAS L
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGL 180

Query: 181 FILPNQ-ENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGV 240
           F++PNQ + S+  E LV   DDVGAGFI GH SQ   +S+ NVDR +D   Q+E G  GV
Sbjct: 181 FVMPNQVKPSDGQEHLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGV 240

Query: 241 SKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKD 300
           SKVIRADQKRFFFDLG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD
Sbjct: 241 SKVIRADQKRFFFDLGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKD 296

Query: 301 RIEGMTGVNVRTVDPPHR 318
           +IEGMTG NVRTVDPP R
Sbjct: 301 KIEGMTGANVRTVDPPQR 296

BLAST of Carg25194 vs. ExPASy Swiss-Prot
Match: P42669 (Transcriptional activator protein Pur-alpha OS=Mus musculus OX=10090 GN=Pura PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 9.0e-16
Identity = 84/297 (28.28%), Postives = 123/297 (41.41%), Query Frame = 0

Query: 3   GSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGN-------------DVELMCKTLQVEHKL 62
           G++ G G S+G   +GS + GG  GGGGGGG+               EL  K + +++K 
Sbjct: 12  GAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGAPGGLQHETQELASKRVDIQNKR 71

Query: 63  FYFDLKENPRGRYLKISE-KTSATRSTIIVPFSGIPWFLDLFNYYI------NSDDPEVF 122
           FY D+K+N +GR+LKI+E      +S + +  S    F D    +I          P   
Sbjct: 72  FYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDL 131

Query: 123 SKELQLDTKAPLFLYLFSLFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDE 182
           ++      +A    +L      +Y D+ EN+RGRFL++        R T+    G    +
Sbjct: 132 AQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRI--------RQTVNRGPGLGSTQ 191

Query: 183 GWTAFRNILAEINEASRLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNV 242
           G T                 LP Q   E  + L  L DD G                   
Sbjct: 192 GQT---------------IALPAQGLIEFRDALAKLIDDYG------------------- 251

Query: 243 DRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 280
                   ++E   L     +  D KRFFFD+GSN  G F+R+SEV    R+SI +P
Sbjct: 252 -------VEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVP 259

BLAST of Carg25194 vs. ExPASy Swiss-Prot
Match: Q00577 (Transcriptional activator protein Pur-alpha OS=Homo sapiens OX=9606 GN=PURA PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-15
Identity = 84/298 (28.19%), Postives = 123/298 (41.28%), Query Frame = 0

Query: 3   GSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGN--------------DVELMCKTLQVEHK 62
           G++ G G S+G   +GS + GG  GGGGGGG+                EL  K + +++K
Sbjct: 12  GAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIQNK 71

Query: 63  LFYFDLKENPRGRYLKISE-KTSATRSTIIVPFSGIPWFLDLFNYYI------NSDDPEV 122
            FY D+K+N +GR+LKI+E      +S + +  S    F D    +I          P  
Sbjct: 72  RFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPD 131

Query: 123 FSKELQLDTKAPLFLYLFSLFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRD 182
            ++      +A    +L      +Y D+ EN+RGRFL++        R T+    G    
Sbjct: 132 LAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRI--------RQTVNRGPGLGST 191

Query: 183 EGWTAFRNILAEINEASRLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLN 242
           +G T                 LP Q   E  + L  L DD G                  
Sbjct: 192 QGQT---------------IALPAQGLIEFRDALAKLIDDYG------------------ 251

Query: 243 VDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 280
                    ++E   L     +  D KRFFFD+GSN  G F+R+SEV    R+SI +P
Sbjct: 252 --------VEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVP 260

BLAST of Carg25194 vs. ExPASy Swiss-Prot
Match: O35295 (Transcriptional activator protein Pur-beta OS=Mus musculus OX=10090 GN=Purb PE=1 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 3.4e-15
Identity = 91/300 (30.33%), Postives = 126/300 (42.00%), Query Frame = 0

Query: 3   GSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 62
           G  GGGG   GG        GG  GG GG     EL  K L +++K FY D+K+N +GR+
Sbjct: 11  GGGGGGGGGPGGFQPAPRGGGGGGGGPGGEQETQELASKRLDIQNKRFYLDVKQNAKGRF 70

Query: 63  LKISE-KTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAP----LFLY 122
           LKI+E     ++S + +  +    F D    +I        S   QL   A         
Sbjct: 71  LKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRA 130

Query: 123 LFSLFIV-----FYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILA 182
           L S F+V     +Y D+ EN+RGRFL++ + +V+R         G     G         
Sbjct: 131 LKSEFLVRENRKYYLDLKENQRGRFLRIRQ-TVNRGGGGF----GGGPGPGGL------- 190

Query: 183 EINEASRLFILPNQENSEHSERLVGLSDDVG------AGFISGHSSQPGPTSDLNVDRQV 242
              ++ +   LP Q   E  + L  L DD G      AG   G +  PG           
Sbjct: 191 ---QSGQTIALPAQGLIEFRDALAKLIDDYGGDEDELAGGPGGGAGGPG----------- 250

Query: 243 DLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 287
                   G L     I  D KRFFFD+G N  G FLR+SEV  + R++I +P     +F
Sbjct: 251 ----GGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKF 280

BLAST of Carg25194 vs. ExPASy Swiss-Prot
Match: Q68A21 (Transcriptional activator protein Pur-beta OS=Rattus norvegicus OX=10116 GN=Purb PE=1 SV=3)

HSP 1 Score: 78.2 bits (191), Expect = 1.9e-13
Identity = 89/300 (29.67%), Postives = 126/300 (42.00%), Query Frame = 0

Query: 4   SSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDV-ELMCKTLQVEHKLFYFDLKENPRGRY 63
           + G  G   GG   GS       GGG GG  +  EL  K L +++K FY D+K+N +GR+
Sbjct: 2   ADGDSGSERGGGGPGSFQPAPRGGGGPGGEQETQELASKRLDIQNKRFYLDVKQNAKGRF 61

Query: 64  LKISE-KTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAP----LFLY 123
           LKI+E     ++S + +  +    F D    +I        S   QL   A         
Sbjct: 62  LKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRA 121

Query: 124 LFSLFIV-----FYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILA 183
           L S F+V     +Y D+ EN+RGRFL++ + +V+R         G     G         
Sbjct: 122 LKSEFLVRENRKYYLDLKENQRGRFLRIRQ-TVNRGGGGF----GGGPGPGGL------- 181

Query: 184 EINEASRLFILPNQENSEHSERLVGLSDDVG------AGFISGHSSQPGPTSDLNVDRQV 243
              ++ +   LP Q   E  + L  L DD G      AG   G +  PG           
Sbjct: 182 ---QSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPG----------- 241

Query: 244 DLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 287
                   G L     I  D KRFFFD+G N  G FLR+SEV  + R++I +P     +F
Sbjct: 242 ----GGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKF 271

BLAST of Carg25194 vs. ExPASy TrEMBL
Match: A0A6J1EUL9 (transcription factor Pur-alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436059 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 3.8e-166
Identity = 305/317 (96.21%), Postives = 305/317 (96.21%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK         
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS
Sbjct: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR
Sbjct: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTGVNVRTVDPPHR
Sbjct: 301 IEGMTGVNVRTVDPPHR 305

BLAST of Carg25194 vs. ExPASy TrEMBL
Match: A0A6J1HVP1 (transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467262 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.7e-164
Identity = 304/319 (95.30%), Postives = 305/319 (95.61%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVT--GGGGGGGNDVELMCKTLQVEHKLFYFDLKENP 60
           MEG+SGGGGVSIGGTTAGSVAVGGVT  GGGGGGGNDVELMCKTLQVEHKLFYFDLKENP
Sbjct: 1   MEGNSGGGGVSIGGTTAGSVAVGGVTGGGGGGGGGNDVELMCKTLQVEHKLFYFDLKENP 60

Query: 61  RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYL 120
           RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK       
Sbjct: 61  RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK------- 120

Query: 121 FSLFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEAS 180
                VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEAS
Sbjct: 121 -----VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEAS 180

Query: 181 RLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLG 240
           RLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLG
Sbjct: 181 RLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLG 240

Query: 241 VSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITK 300
           VSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITK
Sbjct: 241 VSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITK 300

Query: 301 DRIEGMTGVNVRTVDPPHR 318
           DRIEGMTGVNVRTVDPPHR
Sbjct: 301 DRIEGMTGVNVRTVDPPHR 307

BLAST of Carg25194 vs. ExPASy TrEMBL
Match: A0A6J1H823 (transcription factor Pur-alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461388 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 294/318 (92.45%), Postives = 299/318 (94.03%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVT-GGGGGGGNDVELMCKTLQVEHKLFYFDLKENPR 60
           MEG+SGGGG  IGGTTAG VA GGV+ GGGGGGGNDVELMCKTLQVEHKLFYFDLKENPR
Sbjct: 1   MEGNSGGGGAGIGGTTAGGVAAGGVSGGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPR 60

Query: 61  GRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLF 120
           GRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK        
Sbjct: 61  GRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK-------- 120

Query: 121 SLFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASR 180
               VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASR
Sbjct: 121 ----VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASR 180

Query: 181 LFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGV 240
           LF+LPNQENSEHSE LVGLSDDVGAGFISGHSSQP PTSDLNVDRQVDLSAQD+MGNLGV
Sbjct: 181 LFMLPNQENSEHSEHLVGLSDDVGAGFISGHSSQPAPTSDLNVDRQVDLSAQDDMGNLGV 240

Query: 241 SKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKD 300
           SKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKD
Sbjct: 241 SKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKD 300

Query: 301 RIEGMTGVNVRTVDPPHR 318
           RIEGMTGVNVRTVDPPHR
Sbjct: 301 RIEGMTGVNVRTVDPPHR 306

BLAST of Carg25194 vs. ExPASy TrEMBL
Match: A0A1S4DZ49 (transcription factor Pur-alpha 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492403 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.4e-159
Identity = 301/338 (89.05%), Postives = 304/338 (89.94%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEG+SGGGGV IGGTTAG VA GG  G G GGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGG--GAGSGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFL---- 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKA LFL    
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLFLFFLF 120

Query: 121 ----YLFSL-------------FIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSN 180
               YLF L             F VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSN
Sbjct: 121 CFLVYLFVLVGQLLPFLCTHFAFQVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSN 180

Query: 181 RDEGWTAFRNILAEINEASRLFILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSD 240
           RDEGW+AFRNILA+INEASRLFILPNQENSEHSERL GLSDDVGAGFISGHSSQ GPTSD
Sbjct: 181 RDEGWSAFRNILADINEASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSD 240

Query: 241 LNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 300
           LNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP
Sbjct: 241 LNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 300

Query: 301 LSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPHR 318
           LSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPP R
Sbjct: 301 LSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPQR 336

BLAST of Carg25194 vs. ExPASy TrEMBL
Match: A0A6J1JGC7 (transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484871 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.4e-159
Identity = 293/317 (92.43%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEG+SGGGG  IGGTTAG VA GGV+ GGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGAGIGGTTAGGVAAGGVS-GGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK         
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRL
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           F+LPNQENSEHSE LVGLSDDVGAGFISGHSSQP PTSDLNVDRQV+LSAQDEMGNLGVS
Sbjct: 181 FMLPNQENSEHSEHLVGLSDDVGAGFISGHSSQPAPTSDLNVDRQVELSAQDEMGNLGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR
Sbjct: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTGVNVRTVDPPHR
Sbjct: 301 IEGMTGVNVRTVDPPHR 304

BLAST of Carg25194 vs. TAIR 10
Match: AT2G32080.2 (purin-rich alpha 1 )

HSP 1 Score: 450.7 bits (1158), Expect = 9.9e-127
Identity = 239/317 (75.39%), Postives = 264/317 (83.28%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           ME +SGGGG + GG      AV G  GGGGGGG+DVEL+ KTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEANSGGGGGAEGGR-----AVTG--GGGGGGGSDVELVSKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+K         
Sbjct: 61  RYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGS+ DEGW AFRNILAEI+EAS L
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGL 180

Query: 181 FILPNQENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVS 240
           F++PNQ+ S+  E LV   DDVGAGFI GH SQ   +S+ NVDR +D   Q+E G  GVS
Sbjct: 181 FVMPNQKPSDGQEHLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVS 240

Query: 241 KVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDR 300
           KVIRADQKRFFFDLG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+
Sbjct: 241 KVIRADQKRFFFDLGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDK 295

Query: 301 IEGMTGVNVRTVDPPHR 318
           IEGMTG NVRTVDPP R
Sbjct: 301 IEGMTGANVRTVDPPQR 295

BLAST of Carg25194 vs. TAIR 10
Match: AT2G32080.1 (purin-rich alpha 1 )

HSP 1 Score: 446.0 bits (1146), Expect = 2.4e-125
Identity = 239/318 (75.16%), Postives = 264/318 (83.02%), Query Frame = 0

Query: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           ME +SGGGG + GG      AV G  GGGGGGG+DVEL+ KTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEANSGGGGGAEGGR-----AVTG--GGGGGGGSDVELVSKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKAPLFLYLFS 120
           RYLKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+K         
Sbjct: 61  RYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSK--------- 120

Query: 121 LFIVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRL 180
              VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGS+ DEGW AFRNILAEI+EAS L
Sbjct: 121 ---VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGL 180

Query: 181 FILPNQ-ENSEHSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGV 240
           F++PNQ + S+  E LV   DDVGAGFI GH SQ   +S+ NVDR +D   Q+E G  GV
Sbjct: 181 FVMPNQVKPSDGQEHLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGV 240

Query: 241 SKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKD 300
           SKVIRADQKRFFFDLG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD
Sbjct: 241 SKVIRADQKRFFFDLGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKD 296

Query: 301 RIEGMTGVNVRTVDPPHR 318
           +IEGMTG NVRTVDPP R
Sbjct: 301 KIEGMTGANVRTVDPPQR 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022000.13.2e-175100.00Transcription factor Pur-alpha 1, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_022929520.17.8e-16696.21transcription factor Pur-alpha 1-like [Cucurbita moschata][more]
XP_023531642.11.7e-16595.90transcription factor Pur-alpha 1-like [Cucurbita pepo subsp. pepo][more]
KAG6588115.12.3e-16595.90Transcription factor Pur-alpha 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022967890.15.6e-16495.30transcription factor Pur-alpha 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SKZ13.4e-12475.16Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 S... [more]
P426699.0e-1628.28Transcriptional activator protein Pur-alpha OS=Mus musculus OX=10090 GN=Pura PE=... [more]
Q005771.2e-1528.19Transcriptional activator protein Pur-alpha OS=Homo sapiens OX=9606 GN=PURA PE=1... [more]
O352953.4e-1530.33Transcriptional activator protein Pur-beta OS=Mus musculus OX=10090 GN=Purb PE=1... [more]
Q68A211.9e-1329.67Transcriptional activator protein Pur-beta OS=Rattus norvegicus OX=10116 GN=Purb... [more]
Match NameE-valueIdentityDescription
A0A6J1EUL93.8e-16696.21transcription factor Pur-alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436... [more]
A0A6J1HVP12.7e-16495.30transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC11146726... [more]
A0A6J1H8231.2e-15992.45transcription factor Pur-alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461... [more]
A0A1S4DZ493.4e-15989.05transcription factor Pur-alpha 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492... [more]
A0A6J1JGC73.4e-15992.43transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148487... [more]
Match NameE-valueIdentityDescription
AT2G32080.29.9e-12775.39purin-rich alpha 1 [more]
AT2G32080.12.4e-12575.16purin-rich alpha 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006628Purine-rich element binding protein familySMARTSM00712purcoord: 237..298
e-value: 4.4E-24
score: 96.0
coord: 37..98
e-value: 9.7E-23
score: 91.5
coord: 100..179
e-value: 3.4E-17
score: 73.1
IPR006628Purine-rich element binding protein familyPFAMPF04845PurAcoord: 231..293
e-value: 7.5E-13
score: 48.3
coord: 37..176
e-value: 5.0E-18
score: 65.2
IPR006628Purine-rich element binding protein familyPANTHERPTHR12611PUR-TRANSCRIPTIONAL ACTIVATORcoord: 21..304
NoneNo IPR availableGENE3D3.10.450.700coord: 227..301
e-value: 2.4E-19
score: 70.6
coord: 26..99
e-value: 1.8E-18
score: 67.9
coord: 123..179
e-value: 3.1E-17
score: 63.9
NoneNo IPR availablePANTHERPTHR12611:SF0PURINE-RICH BINDING PROTEIN-ALPHA, ISOFORM Bcoord: 21..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25194-RACarg25194-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0003729 mRNA binding
molecular_function GO:0032422 purine-rich negative regulatory element binding
molecular_function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding