Carg24286 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24286
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionactin related protein 2
LocationCarg_Chr15: 461074 .. 462100 (-)
RNA-Seq ExpressionCarg24286
SyntenyCarg24286
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGGATATTTTTTTTTGTTTCTTTGAGATTGGCAAGGAAACCTCGTGTAGGCTCATGGGTGATCTGTGTTCGTGTAGTATTTCTCTTCCGTGCTTTCCTTAACTGCTAGTTCACGCCATCGCAACCCAACCAAATCGCTCTTAGAGAAGGTGGCAGCCACTACCATCTGCTACAAATTCGCATCGCGACGGCATGGATAATCGGAAGGTTATCGTCTGCGACAACGGAACTGGGGTATCTCTTTCTTCTTTTAACCGCTGCTTCAACCTATCGATTTCTCCCCCCTCGATCACCCTCTTCTCGATTCGGTTACCTAAAATTAGATCGTTGATTAATCGCAGTATGTGAAATGTGGATTCGCCGGGGAGAATTTTCCCACATCTGTGTTTCCTTGTGTGGTGGGGAGGCCTCTGCTTCGCTACGAAGAATCGCTCATCGAACAGGAGTTGAAGGTTCGCAATTTCCCTGTTTTGTTTTGTGTCGCTCATCGAACAAAGAATCGCTCCTCCGTTTTCATTTGAATACTGTAGTCACGTGAACTGAGAAATCATCTCTGAATTACTCCTAGGACATTGTTGTCGGAGAAAGCTGTGCAGATTTGCGTCATCAACTTGATATAACCTATCCAGTTCACAATGGAGTTGTCCAAAACTGGGATGATATGGGTCATGTGTGGGACCATGCCTTTTTCAATGAACTGAAGGCAAGGATTTTGTAGCACTTACAATCAGCATTACACATATAATGCCGAACTAAATTGCTTCTGTGTTTGCTGCCAATAGATAGATCCAAAAGAATGTAAAATTTTGCTCACAGACCCACCTCTGAATCCTTCAAGGAACCGTGAACAAATGGTTTGTGAGCTTTGATTTTCTTTCAATTTTGTTTCGATTTCATTAATGAGTTGGGTCAATTTAACCTCACAGGTTGAGACGATGTTTGAGAAGTACAACTTTTCTGGGGTCTTCATTCAAATCCAAGCAGTTCTAACTTTGTATGCTCAAGGTGCTTATAAAGATGAATGA

mRNA sequence

AAAAGGATATTTTTTTTTGTTTCTTTGAGATTGGCAAGGAAACCTCGTGTAGGCTCATGGGTGATCTGTGTTCGTGTAGTATTTCTCTTCCGTGCTTTCCTTAACTGCTAGTTCACGCCATCGCAACCCAACCAAATCGCTCTTAGAGAAGGTGGCAGCCACTACCATCTGCTACAAATTCGCATCGCGACGGCATGGATAATCGGAAGGTTATCGTCTGCGACAACGGAACTGGGTATGTGAAATGTGGATTCGCCGGGGAGAATTTTCCCACATCTGTGTTTCCTTGTGTGGTGGGGAGGCCTCTGCTTCGCTACGAAGAATCGCTCATCGAACAGGAGTTGAAGGACATTGTTGTCGGAGAAAGCTGTGCAGATTTGCGTCATCAACTTGATATAACCTATCCAGTTCACAATGGAGTTGTCCAAAACTGGGATGATATGGGTCATATAGATCCAAAAGAATGTAAAATTTTGCTCACAGACCCACCTCTGAATCCTTCAAGGAACCGTGAACAAATGGTTGAGACGATGTTTGAGAAGTACAACTTTTCTGGGGTCTTCATTCAAATCCAAGCAGTTCTAACTTTGTATGCTCAAGGTGCTTATAAAGATGAATGA

Coding sequence (CDS)

ATGGATAATCGGAAGGTTATCGTCTGCGACAACGGAACTGGGTATGTGAAATGTGGATTCGCCGGGGAGAATTTTCCCACATCTGTGTTTCCTTGTGTGGTGGGGAGGCCTCTGCTTCGCTACGAAGAATCGCTCATCGAACAGGAGTTGAAGGACATTGTTGTCGGAGAAAGCTGTGCAGATTTGCGTCATCAACTTGATATAACCTATCCAGTTCACAATGGAGTTGTCCAAAACTGGGATGATATGGGTCATATAGATCCAAAAGAATGTAAAATTTTGCTCACAGACCCACCTCTGAATCCTTCAAGGAACCGTGAACAAATGGTTGAGACGATGTTTGAGAAGTACAACTTTTCTGGGGTCTTCATTCAAATCCAAGCAGTTCTAACTTTGTATGCTCAAGGTGCTTATAAAGATGAATGA

Protein sequence

MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCADLRHQLDITYPVHNGVVQNWDDMGHIDPKECKILLTDPPLNPSRNREQMVETMFEKYNFSGVFIQIQAVLTLYAQGAYKDE
Homology
BLAST of Carg24286 vs. NCBI nr
Match: KAG7015943.1 (Actin-related protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.8 bits (774), Expect = 1.6e-78
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGHIDPKECKILLTDPPLNPSRNREQMVETMFEKYNFS 120
           DLRHQLDITYPVHNGVVQNWDDMGHIDPKECKILLTDPPLNPSRNREQMVETMFEKYNFS
Sbjct: 61  DLRHQLDITYPVHNGVVQNWDDMGHIDPKECKILLTDPPLNPSRNREQMVETMFEKYNFS 120

Query: 121 GVFIQIQAVLTLYAQGAYKDE 142
           GVFIQIQAVLTLYAQGAYKDE
Sbjct: 121 GVFIQIQAVLTLYAQGAYKDE 141

BLAST of Carg24286 vs. NCBI nr
Match: XP_022939517.1 (actin-related protein 2-like isoform X1 [Cucurbita moschata] >XP_022992829.1 actin-related protein 2-like isoform X1 [Cucurbita maxima] >XP_023550681.1 actin-related protein 2-like isoform X1 [Cucurbita pepo subsp. pepo] >KAG6578359.1 Actin-related protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.5 bits (724), Expect = 1.0e-72
Identity = 136/147 (92.52%), Postives = 136/147 (92.52%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNGVVQNWDDMGH           IDPKECKILLTDPPLNPSRNREQM
Sbjct: 61  DLRHQLDITYPVHNGVVQNWDDMGHVWDHAFFNELKIDPKECKILLTDPPLNPSRNREQM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. NCBI nr
Match: XP_023544804.1 (actin-related protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.6 bits (706), Expect = 1.2e-70
Identity = 131/147 (89.12%), Postives = 135/147 (91.84%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKV+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNG+VQNWDDM H           IDPKECKILLTDPPLNPS+NRE+M
Sbjct: 61  DLRHQLDITYPVHNGIVQNWDDMCHVWDHAFFNELKIDPKECKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. NCBI nr
Match: XP_022956707.1 (actin-related protein 2 [Cucurbita moschata] >XP_022990529.1 actin-related protein 2 [Cucurbita maxima] >KAG6602689.1 Actin-related protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276.6 bits (706), Expect = 1.2e-70
Identity = 131/147 (89.12%), Postives = 135/147 (91.84%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKV+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNG+VQNWDDM H           IDPKECKILLTDPPLNPS+NRE+M
Sbjct: 61  DLRHQLDITYPVHNGIVQNWDDMCHVWDHAFFNELKIDPKECKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. NCBI nr
Match: XP_008441307.1 (PREDICTED: actin-related protein 2 isoform X1 [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 4.7e-70
Identity = 130/147 (88.44%), Postives = 134/147 (91.16%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNR V+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRNVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNG+VQNWDDM H           IDPKECKILLTDPPLNPS+NRE+M
Sbjct: 61  DLRHQLDITYPVHNGIVQNWDDMCHVWDHAFFNELKIDPKECKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. ExPASy Swiss-Prot
Match: Q9LSD6 (Actin-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=ARP2 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 5.9e-68
Identity = 117/147 (79.59%), Postives = 132/147 (89.80%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDN+ V+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESL+EQ++KDIVVGE+C+
Sbjct: 1   MDNKNVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLMEQQVKDIVVGETCS 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           +LRHQLDI YPVHNG+VQNWDDM H           I+P +CKILLTDPPLNPS+NRE+M
Sbjct: 61  ELRHQLDINYPVHNGIVQNWDDMEHVWDHAFYNELKINPSDCKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           +ETMFEKYNF+GVFIQIQAVLTLYAQG
Sbjct: 121 IETMFEKYNFAGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. ExPASy Swiss-Prot
Match: A2YUL5 (Actin-related protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=ARP2 PE=3 SV=2)

HSP 1 Score: 249.6 bits (636), Expect = 2.1e-65
Identity = 115/147 (78.23%), Postives = 128/147 (87.07%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MD+  V+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESL EQEL DIVVG +CA
Sbjct: 1   MDSGNVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLQEQELTDIVVGAACA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGHI-----------DPKECKILLTDPPLNPSRNREQM 120
           DLRHQLD++YPV NG+VQ+WDDMGHI           DP ECKILLTDPPLNP +NRE+M
Sbjct: 61  DLRHQLDVSYPVTNGIVQSWDDMGHIWDHAFYSELKVDPSECKILLTDPPLNPVKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           +ETMFEKYNF+GVFIQ+QAVL+LYAQG
Sbjct: 121 IETMFEKYNFAGVFIQVQAVLSLYAQG 147

BLAST of Carg24286 vs. ExPASy Swiss-Prot
Match: Q6Z256 (Actin-related protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ARP2 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.1e-65
Identity = 115/147 (78.23%), Postives = 128/147 (87.07%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MD+  V+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESL EQEL DIVVG +CA
Sbjct: 1   MDSGNVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLQEQELTDIVVGAACA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGHI-----------DPKECKILLTDPPLNPSRNREQM 120
           DLRHQLD++YPV NG+VQ+WDDMGHI           DP ECKILLTDPPLNP +NRE+M
Sbjct: 61  DLRHQLDVSYPVTNGIVQSWDDMGHIWDHAFYSELKVDPSECKILLTDPPLNPVKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           +ETMFEKYNF+GVFIQ+QAVL+LYAQG
Sbjct: 121 IETMFEKYNFAGVFIQVQAVLSLYAQG 147

BLAST of Carg24286 vs. ExPASy Swiss-Prot
Match: O96621 (Actin-related protein 2 OS=Dictyostelium discoideum OX=44689 GN=arpB PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 2.8e-49
Identity = 88/148 (59.46%), Postives = 111/148 (75.00%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MD+ KVIV DNGTG+VKCGFAG NFPT++FP +VGRP+LR EE +   E+KDI+VG+  A
Sbjct: 1   MDSNKVIVMDNGTGFVKCGFAGANFPTAIFPSMVGRPILRSEEKVENVEIKDIMVGDEAA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGHI------------DPKECKILLTDPPLNPSRNREQ 120
            LR  L ITYP+ NG+++NWDD+ H+            DP ECKILLT+PP+NP  NR++
Sbjct: 61  KLRSMLQITYPLENGIIRNWDDITHVWDYALKEKLKVSDPTECKILLTEPPMNPVANRQK 120

Query: 121 MVETMFEKYNFSGVFIQIQAVLTLYAQG 137
           M+E MFEKY F  V++ IQAVLTLYAQG
Sbjct: 121 MIECMFEKYGFQAVYVAIQAVLTLYAQG 148

BLAST of Carg24286 vs. ExPASy Swiss-Prot
Match: P53487 (Actin-related protein 2 OS=Acanthamoeba castellanii OX=5755 GN=arp2 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 6.8e-48
Identity = 87/146 (59.59%), Postives = 107/146 (73.29%), Query Frame = 0

Query: 2   DNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCAD 61
           D+ KVIVCDNGTG+VKCGFA  NFP S+FP +VGRP+LR EE     E+KDI+VG+  + 
Sbjct: 3   DSSKVIVCDNGTGFVKCGFARSNFPASIFPSMVGRPILRSEEKFDNVEIKDIMVGDEASK 62

Query: 62  LRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQMV 121
           LR  L ITYP+ NG+V+NW+D  H           +DPK+CKILLT+PP+NP  NRE+MV
Sbjct: 63  LRSMLQITYPLDNGIVRNWEDAEHVWNYTFFEKLKVDPKDCKILLTEPPMNPLANREKMV 122

Query: 122 ETMFEKYNFSGVFIQIQAVLTLYAQG 137
           + MFEKY F   +I IQAVLTLYAQG
Sbjct: 123 QVMFEKYGFKAAYIAIQAVLTLYAQG 148

BLAST of Carg24286 vs. ExPASy TrEMBL
Match: A0A6J1JYK0 (actin-related protein 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489043 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.9e-73
Identity = 136/147 (92.52%), Postives = 136/147 (92.52%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNGVVQNWDDMGH           IDPKECKILLTDPPLNPSRNREQM
Sbjct: 61  DLRHQLDITYPVHNGVVQNWDDMGHVWDHAFFNELKIDPKECKILLTDPPLNPSRNREQM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. ExPASy TrEMBL
Match: A0A6J1FH07 (actin-related protein 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445402 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.9e-73
Identity = 136/147 (92.52%), Postives = 136/147 (92.52%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNGVVQNWDDMGH           IDPKECKILLTDPPLNPSRNREQM
Sbjct: 61  DLRHQLDITYPVHNGVVQNWDDMGHVWDHAFFNELKIDPKECKILLTDPPLNPSRNREQM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. ExPASy TrEMBL
Match: A0A6J1JSA3 (actin-related protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111487379 PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.9e-71
Identity = 131/147 (89.12%), Postives = 135/147 (91.84%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKV+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNG+VQNWDDM H           IDPKECKILLTDPPLNPS+NRE+M
Sbjct: 61  DLRHQLDITYPVHNGIVQNWDDMCHVWDHAFFNELKIDPKECKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. ExPASy TrEMBL
Match: A0A6J1GX50 (actin-related protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111458342 PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.9e-71
Identity = 131/147 (89.12%), Postives = 135/147 (91.84%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNRKV+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRKVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNG+VQNWDDM H           IDPKECKILLTDPPLNPS+NRE+M
Sbjct: 61  DLRHQLDITYPVHNGIVQNWDDMCHVWDHAFFNELKIDPKECKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. ExPASy TrEMBL
Match: A0A1S3B342 (actin-related protein 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485464 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.3e-70
Identity = 130/147 (88.44%), Postives = 134/147 (91.16%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDNR V+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA
Sbjct: 1   MDNRNVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           DLRHQLDITYPVHNG+VQNWDDM H           IDPKECKILLTDPPLNPS+NRE+M
Sbjct: 61  DLRHQLDITYPVHNGIVQNWDDMCHVWDHAFFNELKIDPKECKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           VETMFEKYNFSGVFIQIQAVLTLYAQG
Sbjct: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. TAIR 10
Match: AT3G27000.1 (actin related protein 2 )

HSP 1 Score: 258.1 bits (658), Expect = 4.2e-69
Identity = 117/147 (79.59%), Postives = 132/147 (89.80%), Query Frame = 0

Query: 1   MDNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCA 60
           MDN+ V+VCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESL+EQ++KDIVVGE+C+
Sbjct: 1   MDNKNVVVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLMEQQVKDIVVGETCS 60

Query: 61  DLRHQLDITYPVHNGVVQNWDDMGH-----------IDPKECKILLTDPPLNPSRNREQM 120
           +LRHQLDI YPVHNG+VQNWDDM H           I+P +CKILLTDPPLNPS+NRE+M
Sbjct: 61  ELRHQLDINYPVHNGIVQNWDDMEHVWDHAFYNELKINPSDCKILLTDPPLNPSKNREKM 120

Query: 121 VETMFEKYNFSGVFIQIQAVLTLYAQG 137
           +ETMFEKYNF+GVFIQIQAVLTLYAQG
Sbjct: 121 IETMFEKYNFAGVFIQIQAVLTLYAQG 147

BLAST of Carg24286 vs. TAIR 10
Match: AT1G49240.1 (actin 8 )

HSP 1 Score: 136.0 bits (341), Expect = 2.4e-32
Identity = 68/146 (46.58%), Postives = 90/146 (61.64%), Query Frame = 0

Query: 2   DNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCAD 61
           D+ + IVCDNGTG VK GFAG++ P +VFP VVGRP  R+   ++    KD  VG+    
Sbjct: 5   DDIQPIVCDNGTGMVKAGFAGDDAPRAVFPSVVGRP--RHHGVMVGMNQKDAYVGDEAQS 64

Query: 62  LRHQLDITYPVHNGVVQNWDDMG-----------HIDPKECKILLTDPPLNPSRNREQMV 121
            R  L + YP+ +GVV NWDDM             I P+E  +LLT+ PLNP  NRE+M 
Sbjct: 65  KRGILTLKYPIEHGVVSNWDDMEKIWHHTFYNELRIAPEEHPVLLTEAPLNPKANREKMT 124

Query: 122 ETMFEKYNFSGVFIQIQAVLTLYAQG 137
           + MFE +N   +++ IQAVL+LYA G
Sbjct: 125 QIMFETFNSPAMYVAIQAVLSLYASG 148

BLAST of Carg24286 vs. TAIR 10
Match: AT2G42100.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 2.4e-32
Identity = 69/146 (47.26%), Postives = 90/146 (61.64%), Query Frame = 0

Query: 2   DNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCAD 61
           D    IVCDNGTG VK GFAG++ P +VFP VVGRP  R+   ++  + KD  VG+    
Sbjct: 6   DESVAIVCDNGTGMVKAGFAGDDAPRAVFPSVVGRP--RHRGVMVGMDEKDTFVGDEAQA 65

Query: 62  LRHQLDITYPVHNGVVQNWDDMGHI-----------DPKECKILLTDPPLNPSRNREQMV 121
            R  L + YP+ +GVV NWDDM  I           +P+E  ILLT+ PLNP  NRE+M 
Sbjct: 66  RRGILSLKYPIEHGVVSNWDDMEKIWHHTFYNELRLEPEEHPILLTEAPLNPKVNREKMT 125

Query: 122 ETMFEKYNFSGVFIQIQAVLTLYAQG 137
           + MFE + F  ++I IQAVL+LY+ G
Sbjct: 126 QIMFESFAFPSMYIGIQAVLSLYSSG 149

BLAST of Carg24286 vs. TAIR 10
Match: AT3G18780.1 (actin 2 )

HSP 1 Score: 136.0 bits (341), Expect = 2.4e-32
Identity = 68/146 (46.58%), Postives = 90/146 (61.64%), Query Frame = 0

Query: 2   DNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCAD 61
           D+ + IVCDNGTG VK GFAG++ P +VFP VVGRP  R+   ++    KD  VG+    
Sbjct: 5   DDIQPIVCDNGTGMVKAGFAGDDAPRAVFPSVVGRP--RHHGVMVGMNQKDAYVGDEAQS 64

Query: 62  LRHQLDITYPVHNGVVQNWDDMG-----------HIDPKECKILLTDPPLNPSRNREQMV 121
            R  L + YP+ +GVV NWDDM             I P+E  +LLT+ PLNP  NRE+M 
Sbjct: 65  KRGILTLKYPIEHGVVSNWDDMEKIWHHTFYNELRIAPEEHPVLLTEAPLNPKANREKMT 124

Query: 122 ETMFEKYNFSGVFIQIQAVLTLYAQG 137
           + MFE +N   +++ IQAVL+LYA G
Sbjct: 125 QIMFETFNSPAMYVAIQAVLSLYASG 148

BLAST of Carg24286 vs. TAIR 10
Match: AT3G18780.2 (actin 2 )

HSP 1 Score: 136.0 bits (341), Expect = 2.4e-32
Identity = 68/146 (46.58%), Postives = 90/146 (61.64%), Query Frame = 0

Query: 2   DNRKVIVCDNGTGYVKCGFAGENFPTSVFPCVVGRPLLRYEESLIEQELKDIVVGESCAD 61
           D+ + IVCDNGTG VK GFAG++ P +VFP VVGRP  R+   ++    KD  VG+    
Sbjct: 5   DDIQPIVCDNGTGMVKAGFAGDDAPRAVFPSVVGRP--RHHGVMVGMNQKDAYVGDEAQS 64

Query: 62  LRHQLDITYPVHNGVVQNWDDMG-----------HIDPKECKILLTDPPLNPSRNREQMV 121
            R  L + YP+ +GVV NWDDM             I P+E  +LLT+ PLNP  NRE+M 
Sbjct: 65  KRGILTLKYPIEHGVVSNWDDMEKIWHHTFYNELRIAPEEHPVLLTEAPLNPKANREKMT 124

Query: 122 ETMFEKYNFSGVFIQIQAVLTLYAQG 137
           + MFE +N   +++ IQAVL+LYA G
Sbjct: 125 QIMFETFNSPAMYVAIQAVLSLYASG 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015943.11.6e-78100.00Actin-related protein 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022939517.11.0e-7292.52actin-related protein 2-like isoform X1 [Cucurbita moschata] >XP_022992829.1 act... [more]
XP_023544804.11.2e-7089.12actin-related protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022956707.11.2e-7089.12actin-related protein 2 [Cucurbita moschata] >XP_022990529.1 actin-related prote... [more]
XP_008441307.14.7e-7088.44PREDICTED: actin-related protein 2 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LSD65.9e-6879.59Actin-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=ARP2 PE=1 SV=1[more]
A2YUL52.1e-6578.23Actin-related protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=ARP2 PE=3 SV=2[more]
Q6Z2562.1e-6578.23Actin-related protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ARP2 PE=3 SV... [more]
O966212.8e-4959.46Actin-related protein 2 OS=Dictyostelium discoideum OX=44689 GN=arpB PE=1 SV=1[more]
P534876.8e-4859.59Actin-related protein 2 OS=Acanthamoeba castellanii OX=5755 GN=arp2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JYK04.9e-7392.52actin-related protein 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489... [more]
A0A6J1FH074.9e-7392.52actin-related protein 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JSA35.9e-7189.12actin-related protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111487379 PE=3 SV=1[more]
A0A6J1GX505.9e-7189.12actin-related protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111458342 PE=3 SV=1[more]
A0A1S3B3422.3e-7088.44actin-related protein 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485464 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G27000.14.2e-6979.59actin related protein 2 [more]
AT1G49240.12.4e-3246.58actin 8 [more]
AT2G42100.12.4e-3247.26Actin-like ATPase superfamily protein [more]
AT3G18780.12.4e-3246.58actin 2 [more]
AT3G18780.22.4e-3246.58actin 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004000Actin familyPRINTSPR00190ACTINcoord: 25..34
score: 49.69
coord: 50..61
score: 32.17
coord: 62..84
score: 46.81
IPR004000Actin familyPFAMPF00022Actincoord: 2..136
e-value: 8.7E-29
score: 100.3
IPR004000Actin familyPANTHERPTHR11937ACTINcoord: 1..138
NoneNo IPR availableGENE3D3.30.420.40coord: 1..141
e-value: 1.7E-44
score: 153.4
NoneNo IPR availablePANTHERPTHR11937:SF439ACTIN-RELATED PROTEIN 2coord: 1..138
NoneNo IPR availableCDDcd00012NBD_sugar-kinase_HSP70_actincoord: 7..141
e-value: 2.3067E-6
score: 42.9612
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 1..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24286-RACarg24286-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034314 Arp2/3 complex-mediated actin nucleation
cellular_component GO:0005885 Arp2/3 protein complex
molecular_function GO:0051015 actin filament binding