Carg21947 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg21947 vs. NCBI nr
Match: XP_022933888.1 (protein ALP1-like [Cucurbita moschata] >KAG7021950.1 Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 971.1 bits (2509), Expect = 3.5e-279
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120
           LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH
Sbjct: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120

Query: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180
           RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP
Sbjct: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180

Query: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240
           VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL
Sbjct: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240

Query: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300
           AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD
Sbjct: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300

Query: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360
           PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ
Sbjct: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360

Query: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420
           NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE
Sbjct: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420

Query: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL
Sbjct: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 473

BLAST of Carg21947 vs. NCBI nr
Match: KAG6588056.1 (Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 969.9 bits (2506), Expect = 7.8e-279
Identity = 472/473 (99.79%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEISSFPFLNQDDL+PIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE
Sbjct: 1   MEISSFPFLNQDDLVPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120
           LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH
Sbjct: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120

Query: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180
           RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP
Sbjct: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180

Query: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240
           VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL
Sbjct: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240

Query: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300
           AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD
Sbjct: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300

Query: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360
           PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ
Sbjct: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360

Query: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420
           NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE
Sbjct: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420

Query: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL
Sbjct: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 473

BLAST of Carg21947 vs. NCBI nr
Match: XP_023529395.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957.6 bits (2474), Expect = 4.0e-275
Identity = 466/473 (98.52%), Postives = 470/473 (99.37%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEISSFPFLNQDDLLPIFNLFSEMDDNFSVN SPKKRRRQ++DDDD QTQFNKTSFDDDE
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNHSPKKRRRQDNDDDDDQTQFNKTSFDDDE 60

Query: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120
           LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSP KGGAGTQH
Sbjct: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPVKGGAGTQH 120

Query: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180
           RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMIC+ELDSTVMKKDTMLRVAIP
Sbjct: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICKELDSTVMKKDTMLRVAIP 180

Query: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240
           VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPE+SKL
Sbjct: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEDSKL 240

Query: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300
           AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD
Sbjct: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300

Query: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360
           PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ
Sbjct: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360

Query: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420
           NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE
Sbjct: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420

Query: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL
Sbjct: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 473

BLAST of Carg21947 vs. NCBI nr
Match: XP_022967341.1 (protein ALP1-like [Cucurbita maxima])

HSP 1 Score: 949.1 bits (2452), Expect = 1.4e-272
Identity = 465/473 (98.31%), Postives = 468/473 (98.94%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEISSFPFLNQDDLLPIFNLFSEMDDNFSVN SPKKRRRQ+DDDDD QTQFNKTSFDDDE
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDD-QTQFNKTSFDDDE 60

Query: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120
           LLKLPFWFDDDKQQHWIMEQKPEFQV DENLTQFV KKPRRATPENTHSSPAKGGAGTQH
Sbjct: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAKGGAGTQH 120

Query: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180
           RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP
Sbjct: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180

Query: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240
           VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPE+SKL
Sbjct: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEDSKL 240

Query: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300
           AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD
Sbjct: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300

Query: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360
           PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ
Sbjct: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360

Query: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420
           NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE
Sbjct: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420

Query: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           MRKERFDPELKFEFFDDEMVPENNGVRSASAI ARDHI+HNLLHHGLAGTGFL
Sbjct: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLAGTGFL 472

BLAST of Carg21947 vs. NCBI nr
Match: XP_038880089.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 809.7 bits (2090), Expect = 1.3e-230
Identity = 411/489 (84.05%), Postives = 436/489 (89.16%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDN-------FSVNQSPKKRRRQNDDDDDHQTQFNK 60
           MEISSFPFLNQ++LLPIFNLFS+MD+N       FSVNQSPKKRRR +++ DDH +QFN 
Sbjct: 1   MEISSFPFLNQEELLPIFNLFSDMDNNHNNTNATFSVNQSPKKRRRSDENGDDH-SQFNN 60

Query: 61  TSF--DDDELLKLPFWFDDDKQQHWIM---EQKP--EFQVSDENLTQFVPKKPRRATPEN 120
            SF  +D+ L KLP WF  + Q++WIM   E KP  EF +SD+N TQF  KKPRR T EN
Sbjct: 61  ISFTENDEALQKLPCWF--ESQENWIMDSEEPKPRNEFHLSDQNPTQF-SKKPRRTTAEN 120

Query: 121 THSSPAKG--GAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQEL 180
              SPAK   G G Q RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+EL
Sbjct: 121 --GSPAKNPTGGGAQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKEL 180

Query: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240
           DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR
Sbjct: 181 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 240

Query: 241 KVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 300
           KVLMPKFLQWP+ESKL KIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE
Sbjct: 241 KVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 300

Query: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGN 360
           RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKS LFERANMGLL DV IVGN
Sbjct: 301 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGN 360

Query: 361 SGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQE 420
           SGYPL DWLLVPY+ QNLTWTQH FNEKV EIQ AAK AFGRLKGRW+CLQKRTEVKLQE
Sbjct: 361 SGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQE 420

Query: 421 LPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLH 474
           LPVVLGACCVLHNICE+RKE+FDP+LKFE +DDEMVPENNG+RS SAIQARDHIAHNLLH
Sbjct: 421 LPVVLGACCVLHNICEIRKEKFDPDLKFELYDDEMVPENNGLRSVSAIQARDHIAHNLLH 480

BLAST of Carg21947 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 6.2e-37
Identity = 106/330 (32.12%), Postives = 163/330 (49.39%), Query Frame = 0

Query: 128 RSKDWWDQCNHPDF----PDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVA----I 187
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 188 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESK 247
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 248 LAKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 307
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 308 QGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGL--------------LKDVSI 367
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G               L++  I
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREY-I 289

Query: 368 VGNSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEV- 427
           VG+SG+PL  WLL PY  +  +  Q  FN++ SE   AA+ A  +LK RW  +     + 
Sbjct: 290 VGDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMP 349

Query: 428 KLQELPVVLGACCVLHN-ICEMRKERFDPE 430
               LP ++  CC+LHN I +M  +  D +
Sbjct: 350 DRNRLPRIIFVCCLLHNIIIDMEDQTLDDQ 366

BLAST of Carg21947 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.1e-33
Identity = 93/314 (29.62%), Postives = 155/314 (49.36%), Query Frame = 0

Query: 131 DWWD----QCNHPDFPDEE---FRRAFRMSKATFDMICQELDSTVMKKDTMLRVAI---- 190
           DWWD    + + P  P +E   F+  FR SK TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 191 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES 250
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WP+  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 251 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 310
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 311 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVS-------------IVGNS 370
            D    F ++  GWPG M   ++L+ S  F+      + D +             +VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 371 GYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQK-RTEVKLQE 419
            YPL  WL+ P+ + + + +  AFNE+  +++  A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Carg21947 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.2e-17
Identity = 70/261 (26.82%), Postives = 119/261 (45.59%), Query Frame = 0

Query: 163 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 222
           EL    + + T    AI    +V   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 223 IRKVLMPKFLQWP-EESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 282
           + +    +F+++P +E+ +  +K EF  ++G+P V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNR- 170

Query: 283 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSI 342
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+ KD  +
Sbjct: 171 ------KGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWL 230

Query: 343 VGNSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVK 402
           +G+S + L  WL+ P      T  ++ +N   S      +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 403 LQELPV----VLGACCVLHNI 419
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Carg21947 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 69/261 (26.44%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 163 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 222
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 223 IRKVLMPKFLQWP-EESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 282
           + +    +F+ +P +E+ +  +K EF  ++GIP V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNR- 170

Query: 283 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSI 342
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+ K+  +
Sbjct: 171 ------KGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWL 230

Query: 343 VGNSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVK 402
           +G+S + L  WL+ P      T  ++ +N   S      +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 403 LQELPV----VLGACCVLHNI 419
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Carg21947 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 69/261 (26.44%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 163 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 222
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 223 IRKVLMPKFLQWP-EESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 282
           + +    +F+ +P +E+ +  +K EF  ++G+P V G++   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR- 170

Query: 283 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSI 342
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+ KD  +
Sbjct: 171 ------KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWL 230

Query: 343 VGNSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVK 402
           +G+S + L  WLL P      T  ++ +N   S      +     L  R+ CL   ++  
Sbjct: 231 LGDSSFFLHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLD-GSKGA 290

Query: 403 LQELPV----VLGACCVLHNI 419
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Carg21947 vs. ExPASy TrEMBL
Match: A0A6J1F642 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 1.7e-279
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120
           LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH
Sbjct: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120

Query: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180
           RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP
Sbjct: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180

Query: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240
           VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL
Sbjct: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240

Query: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300
           AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD
Sbjct: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300

Query: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360
           PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ
Sbjct: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360

Query: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420
           NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE
Sbjct: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420

Query: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL
Sbjct: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 473

BLAST of Carg21947 vs. ExPASy TrEMBL
Match: A0A6J1HRQ5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 6.9e-273
Identity = 465/473 (98.31%), Postives = 468/473 (98.94%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEISSFPFLNQDDLLPIFNLFSEMDDNFSVN SPKKRRRQ+DDDDD QTQFNKTSFDDDE
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDD-QTQFNKTSFDDDE 60

Query: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGTQH 120
           LLKLPFWFDDDKQQHWIMEQKPEFQV DENLTQFV KKPRRATPENTHSSPAKGGAGTQH
Sbjct: 61  LLKLPFWFDDDKQQHWIMEQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAKGGAGTQH 120

Query: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180
           RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP
Sbjct: 121 RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIP 180

Query: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKL 240
           VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPE+SKL
Sbjct: 181 VRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEDSKL 240

Query: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300
           AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD
Sbjct: 241 AKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVD 300

Query: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360
           PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ
Sbjct: 301 PAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQ 360

Query: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420
           NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE
Sbjct: 361 NLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICE 420

Query: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           MRKERFDPELKFEFFDDEMVPENNGVRSASAI ARDHI+HNLLHHGLAGTGFL
Sbjct: 421 MRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLAGTGFL 472

BLAST of Carg21947 vs. ExPASy TrEMBL
Match: A0A6J1HAH8 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111461585 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.0e-228
Identity = 401/475 (84.42%), Postives = 423/475 (89.05%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEI+SFPFLNQD+ LPI N FSEM DNFSVNQSPKKRRR  D+ DD       +    ++
Sbjct: 1   MEITSFPFLNQDEFLPISNFFSEM-DNFSVNQSPKKRRRPTDNGDD------GSGNGGND 60

Query: 61  LLKLPFWFD--DDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGT 120
           LL  PFW +  D++++HW+M+   EFQV        + KKPRR TP+N +SSPAKGGAG 
Sbjct: 61  LLNHPFWVENRDEQREHWVMD-SDEFQV--------LSKKPRRGTPQNDNSSPAKGGAGA 120

Query: 121 QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVA 180
             RRLWVK+RSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMIC+ELDSTVMKKDTMLRVA
Sbjct: 121 HQRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDSTVMKKDTMLRVA 180

Query: 181 IPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES 240
           IPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES
Sbjct: 181 IPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES 240

Query: 241 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 300
           KL KIKQEFESISGIPKVGG+IYTTHIPIIAPKN+VAAYFNKRHTERNQKTSYSITVQGV
Sbjct: 241 KLTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQKTSYSITVQGV 300

Query: 301 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYS 360
           VDPAGVFTDVCIGWPGSMPDD VLEKSALFERANMGLL DV IVGNSGYPL DWLLVPY+
Sbjct: 301 VDPAGVFTDVCIGWPGSMPDDHVLEKSALFERANMGLLNDVFIVGNSGYPLMDWLLVPYT 360

Query: 361 AQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNI 420
            QNLTWTQHAFNEKV EIQ AAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNI
Sbjct: 361 LQNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNI 420

Query: 421 CEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           CEMRKERFD ELKFE FDDEMVPE+NGVRS SAIQ RDHIAHNLLHHGLAGTGFL
Sbjct: 421 CEMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGLAGTGFL 459

BLAST of Carg21947 vs. ExPASy TrEMBL
Match: A0A6J1JHN7 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111485185 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.2e-227
Identity = 401/475 (84.42%), Postives = 423/475 (89.05%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60
           MEI+S PFLNQD+ LPI N FSE+ DNFSVNQS KKRRR  D+ DD       +    ++
Sbjct: 1   MEITSVPFLNQDEFLPISNFFSEI-DNFSVNQSLKKRRRPTDNGDD------GSGNGGND 60

Query: 61  LLKLPFWFD--DDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGAGT 120
           LL LPFW +  D++Q+HW+M+   EFQV        + KKPRR TP+N +SSPAKGGAG 
Sbjct: 61  LLNLPFWVENRDEQQEHWVMD-SDEFQV--------LSKKPRRGTPQNDNSSPAKGGAGA 120

Query: 121 QHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVA 180
             RRLWVK+RSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMIC+ELDSTVMKKDTMLRVA
Sbjct: 121 HQRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDSTVMKKDTMLRVA 180

Query: 181 IPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES 240
           IPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES
Sbjct: 181 IPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES 240

Query: 241 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 300
           KL KIKQEFESISGIPKVGG+IYTTHIPIIAPKN+VAAYFNKRHTERNQKTSYSITVQGV
Sbjct: 241 KLTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQKTSYSITVQGV 300

Query: 301 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYS 360
           VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYPL DWLLVPY+
Sbjct: 301 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLMDWLLVPYT 360

Query: 361 AQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNI 420
            QNLTWTQHAFNEKV EIQ AAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNI
Sbjct: 361 LQNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNI 420

Query: 421 CEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 474
           CEMRKERFD ELKFE FDDEMVPE+NGVRS SAIQ RDHIAHNLLHHGLAGTGFL
Sbjct: 421 CEMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGLAGTGFL 459

BLAST of Carg21947 vs. ExPASy TrEMBL
Match: A0A1S3BPN5 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103492343 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.4e-227
Identity = 398/490 (81.22%), Postives = 429/490 (87.55%), Query Frame = 0

Query: 1   MEISSFPFLNQDDLLPIFNLFSEMDDN----FSVNQSPKKRRRQNDDDDDHQTQFNKTSF 60
           MEISSFPFLNQ++ LPIFNLFS+MD+N    F+VN +PKKRRR + + DD    FN  SF
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSDMDNNPTTPFNVNPTPKKRRRSDPNSDD----FNNFSF 60

Query: 61  -------DDDELLKLPFWFD--DDKQQHWIMEQKPEFQVSDENLTQFVPKKPRRATPENT 120
                   DD LLKLP WFD   +  Q W+M+ +     +D +L+  +PKKPRRA+PEN 
Sbjct: 61  TDENDEPTDDPLLKLPCWFDPQPESPQSWLMDSQKPKPTNDFHLSDQIPKKPRRASPEN- 120

Query: 121 HSSPAK----GGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQE 180
             SP K    GG GTQ RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+E
Sbjct: 121 -PSPVKNNPAGGGGTQQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKE 180

Query: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240
           LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI
Sbjct: 181 LDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAI 240

Query: 241 RKVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHT 300
           RKVLMPKFLQWP+ESKL KIKQEFESISGIPKVGGSIYTTHIPIIAP+NNVAAYFNKRHT
Sbjct: 241 RKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPRNNVAAYFNKRHT 300

Query: 301 ERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVG 360
           ERNQKTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS L+ERA+MGLL DV +VG
Sbjct: 301 ERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVG 360

Query: 361 NSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQ 420
           NSGYPL DWLLVPY+ QNLTWTQH FNEKV EIQ AAKAAFGRLKGRWTCLQKRTEVKLQ
Sbjct: 361 NSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQ 420

Query: 421 ELPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLL 474
           ELPVVLGACCVLHNICEMRKE+FDPELKFE +DDEM+PENNG+RS SAIQARDHIAHNLL
Sbjct: 421 ELPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLL 480

BLAST of Carg21947 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 566.6 bits (1459), Expect = 1.9e-161
Identity = 266/370 (71.89%), Postives = 316/370 (85.41%), Query Frame = 0

Query: 105 ENTHSSPAKGGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQEL 164
           E T S   +G    Q RRLWVKDRS+ WW++C+  D+P+E+F++AFRMSK+TF++IC EL
Sbjct: 134 EITGSGSVRGTGSGQQRRLWVKDRSRAWWEECSRLDYPEEDFKKAFRMSKSTFELICDEL 193

Query: 165 DSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIR 224
           +S V K+DT LR AIPVRQRVAVCIWRLATGEPLRLVSK+FGLGISTCHKLVLEVC AI+
Sbjct: 194 NSAVAKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIK 253

Query: 225 KVLMPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTE 284
            VLMPK+LQWP++  L  I++ FES+SGIP V GS+YTTHIPIIAPK +VA+YFNKRHTE
Sbjct: 254 DVLMPKYLQWPDDESLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTE 313

Query: 285 RNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANM-GLLKDVSIVG 344
           RNQKTSYSIT+Q VV+P GVFTD+CIGWPGSMPDD+VLEKS L++RAN  GLLK + + G
Sbjct: 314 RNQKTSYSITIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGMWVAG 373

Query: 345 NSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQ 404
             G+PL DW+LVPY+ QNLTWTQHAFNEK+SE+QG AK AFGRLKGRW CLQKRTEVKLQ
Sbjct: 374 GPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQKRTEVKLQ 433

Query: 405 ELPVVLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLL 464
           +LP VLGACCVLHNICEMR+E+ +PEL  E  DDE++PE N +RS +A++ARD I+HNLL
Sbjct: 434 DLPTVLGACCVLHNICEMREEKMEPELMVEVIDDEVLPE-NVLRSVNAMKARDTISHNLL 493

Query: 465 HHGLAGTGFL 474
           HHGLAGT FL
Sbjct: 494 HHGLAGTSFL 502

BLAST of Carg21947 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 540.4 bits (1391), Expect = 1.4e-153
Identity = 300/540 (55.56%), Postives = 359/540 (66.48%), Query Frame = 0

Query: 1   MEISSFPF--LNQDDLLPIFNLFSEMDDNFSV-----------NQSPKKRRRQNDDDD-- 60
           MEISSFPF  L  D+      LF +MD + S            N + KKR R++D+    
Sbjct: 1   MEISSFPFPYLQDDECSHFLGLFQDMDSSPSTFGLEGFNSNDNNTNQKKRPRKDDEGGGG 60

Query: 61  --------DHQTQFNKTSFDDDELLKLPFWFDDD---KQQHWIMEQKPEFQVSDENLTQF 120
                         NK +F D  +L      D++   +Q+ W  E   E  + + N  + 
Sbjct: 61  GGGGTEVLGAVNGNNKAAFGD--ILATLLLLDEEAKQQQEQWDFEFIKEKSLLEANHKKK 120

Query: 121 V------------------------PKKPRR-------------------ATPENTHSSP 180
           V                         K+ R+                   A P  T    
Sbjct: 121 VKTMDGYYNQMQDHYSAAGETDGSRSKRARKTAVAAVVSAVASGADTTGLAAPVPTADIA 180

Query: 181 AKGGAGTQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKK 240
           +  G+G  HRRLWVK+R+ DWWD+ + PDFP++EFRR FRMSK+TF++IC+ELD+TV KK
Sbjct: 181 SGSGSGPSHRRLWVKERTTDWWDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTKK 240

Query: 241 DTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKF 300
           +TMLR AIP  +RV VC+WRLATG PLR VS+RFGLGISTCHKLV+EVC AI  VLMPK+
Sbjct: 241 NTMLRDAIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKY 300

Query: 301 LQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSY 360
           L WP +S++   K +FES+  IP V GSIYTTHIPIIAPK +VAAYFNKRHTERNQKTSY
Sbjct: 301 LLWPSDSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSY 360

Query: 361 SITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALF-ERANMGLLKDVSIVGNSGYPLT 420
           SITVQGVV+  G+FTDVCIG PGS+ DDQ+LEKS+L  +RA  G+L+D  IVGNSG+PLT
Sbjct: 361 SITVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSWIVGNSGFPLT 420

Query: 421 DWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLG 471
           D+LLVPY+ QNLTWTQHAFNE + EIQG A AAF RLKGRW CLQKRTEVKLQ+LP VLG
Sbjct: 421 DYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLG 480

BLAST of Carg21947 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 156.8 bits (395), Expect = 4.4e-38
Identity = 106/330 (32.12%), Postives = 163/330 (49.39%), Query Frame = 0

Query: 128 RSKDWWDQCNHPDF----PDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVA----I 187
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 188 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESK 247
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 248 LAKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 307
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 308 QGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGL--------------LKDVSI 367
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G               L++  I
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREY-I 289

Query: 368 VGNSGYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEV- 427
           VG+SG+PL  WLL PY  +  +  Q  FN++ SE   AA+ A  +LK RW  +     + 
Sbjct: 290 VGDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMP 349

Query: 428 KLQELPVVLGACCVLHN-ICEMRKERFDPE 430
               LP ++  CC+LHN I +M  +  D +
Sbjct: 350 DRNRLPRIIFVCCLLHNIIIDMEDQTLDDQ 366

BLAST of Carg21947 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 146.0 bits (367), Expect = 7.8e-35
Identity = 93/314 (29.62%), Postives = 155/314 (49.36%), Query Frame = 0

Query: 131 DWWD----QCNHPDFPDEE---FRRAFRMSKATFDMICQELDSTVMKKDTMLRVAI---- 190
           DWWD    + + P  P +E   F+  FR SK TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 191 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEES 250
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WP+  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 251 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 310
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 311 VDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVS-------------IVGNS 370
            D    F ++  GWPG M   ++L+ S  F+      + D +             +VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 371 GYPLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQK-RTEVKLQE 419
            YPL  WL+ P+ + + + +  AFNE+  +++  A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Carg21947 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 103.2 bits (256), Expect = 5.8e-22
Identity = 80/309 (25.89%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 143 DEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVS 202
           D  +R  + +S   F  +  +L   +    T   +++P    VA+ + RLA G   + ++
Sbjct: 114 DARWRSLYGLSYPVFITVVDKLKPFI----TASNLSLPADYAVAMVLSRLAHGCSAKTLA 173

Query: 203 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP-EESKLAKIKQEFESISGIPKVGGSIY 262
            R+ L      K+   V   +   L P+F++ P  + +L +  Q FE ++ +P + G+I 
Sbjct: 174 SRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAID 233

Query: 263 TTHIPIIAPKNNVAAYFNKRHTERNQKTSY-------SITVQGVVDPAGVFTDVCIGWPG 322
           +T + +            +R T+ N +  Y       ++ +Q V D   +F DVC+  PG
Sbjct: 234 STPVKL------------RRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPG 293

Query: 323 SMPDDQVLEKSALFERANMGLL---KDVS---------IVGNSGYPLTDWLLVPYSAQNL 382
              D      S L++R   G +   K ++         IVG+  YPL  +L+ P+S    
Sbjct: 294 GEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGS 353

Query: 383 -TWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEM 431
            T  ++ F+  + + +     A G LK RW  LQ    V +   P  + ACCVLHN+C++
Sbjct: 354 GTPPENLFDGMLMKGRSVVVEAIGLLKARWKILQS-LNVGVNHAPQTIVACCVLHNLCQI 404