Carg21791 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21791
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Location	Carg_Chr09: 1287075 .. 1287602 (+)
RNA-Seq Expression	Carg21791
Synteny	Carg21791

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACATCATAGGAACCCTATGCGCGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCAGGCACGAAATCGGCTTGGGAGGAGTTCTCGGTAGCTTCAGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTACGCGAAGAACGAGGCGTTTAACTGCAACAATGGCGATGTATTCAAGTGGAAACATTTCTGGAAAGTGTTGGCAGAACAGTTCAACATTGAGGATTACGGGTTCGAAGAGGGCGAGAAATTGACGTTGGTGGAGTTGATGAAGGACAAAAGCAAAGTGTGGGAAGAGATCGTGAAGGAGAATCAGCTGCAACCAACCCGACTGGAGGAGATTGGTAATTGGTGGTTCGTAGATATAATTTTGAGCATTTCAGGTAACATAGATAGCATGAACAAGAGCAAGGAGCATGGATTTCTGGGCTTCAGGAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGATCAAGGCTTTCAAAATTGTGCCTTGA

mRNA sequence

ATGAACATCATAGGAACCCTATGCGCGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCAGGCACGAAATCGGCTTGGGAGGAGTTCTCGGTAGCTTCAGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTACGCGAAGAACGAGGCGTTTAACTGCAACAATGGCGATGTATTCAAGTGGAAACATTTCTGGAAAGTGTTGGCAGAACAGTTCAACATTGAGGATTACGGGTTCGAAGAGGGCGAGAAATTGACGTTGGTGGAGTTGATGAAGGACAAAAGCAAAGTGTGGGAAGAGATCGTGAAGGAGAATCAGCTGCAACCAACCCGACTGGAGGAGATTGGTAATTGGTGGTTCGTAGATATAATTTTGAGCATTTCAGGTAACATAGATAGCATGAACAAGAGCAAGGAGCATGGATTTCTGGGCTTCAGGAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGATCAAGGCTTTCAAAATTGTGCCTTGA

Coding sequence (CDS)

ATGAACATCATAGGAACCCTATGCGCGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCAGGCACGAAATCGGCTTGGGAGGAGTTCTCGGTAGCTTCAGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTACGCGAAGAACGAGGCGTTTAACTGCAACAATGGCGATGTATTCAAGTGGAAACATTTCTGGAAAGTGTTGGCAGAACAGTTCAACATTGAGGATTACGGGTTCGAAGAGGGCGAGAAATTGACGTTGGTGGAGTTGATGAAGGACAAAAGCAAAGTGTGGGAAGAGATCGTGAAGGAGAATCAGCTGCAACCAACCCGACTGGAGGAGATTGGTAATTGGTGGTTCGTAGATATAATTTTGAGCATTTCAGGTAACATAGATAGCATGAACAAGAGCAAGGAGCATGGATTTCTGGGCTTCAGGAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGATCAAGGCTTTCAAAATTGTGCCTTGA

Protein sequence

MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP

Homology

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BLAST of Carg21791 vs. NCBI nr
Match: KAG7024289.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.1 bits (954), Expect = 2.7e-99
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 175

[Close]

BLAST of Carg21791 vs. NCBI nr
Match: XP_022977039.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 372.1 bits (954), Expect = 2.7e-99
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 219 MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. NCBI nr
Match: KAG6591410.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 372.1 bits (954), Expect = 2.7e-99
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 219 MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. NCBI nr
Match: XP_022936254.1 (uncharacterized protein LOC111442928 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 5.9e-99
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAIC+HEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 219 MNIIGTLCAYAAICRHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. NCBI nr
Match: XP_023535925.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.5 bits (950), Expect = 7.7e-99
Identity = 173/175 (98.86%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFS+ASDADLIAEQHIWT+VDPYAKNEAF
Sbjct: 219 MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSIASDADLIAEQHIWTSVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 2.0e-81
Identity = 136/175 (77.71%), Postives = 150/175 (85.71%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNI+GTLC YAAICKHEG PL FPG+K AWE F  ASDADLIAEQ IW AVDPYAKNEAF
Sbjct: 214 MNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAF 273

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNN D+FKWKH WK+LAEQF IE+YGFEEG+ L LVE+MK K +VWEE+VKENQLQ  +
Sbjct: 274 NCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKK 333

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEE+G WWF D+IL + G IDSMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 334 LEEVGVWWFADVILGVEGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of Carg21791 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.0e-73
Identity = 124/175 (70.86%), Postives = 146/175 (83.43%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MN++GTLC YAAICKHEG  L+FPG K AW+ +S  SDADLIAE  IW AVDPYAKNEAF
Sbjct: 214 MNLVGTLCVYAAICKHEGAVLRFPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAF 273

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           N +NGDVFKWKHFWKVLAEQF +E   +EEG+++ L +LMKDK  VW++IV+EN L  T+
Sbjct: 274 NVSNGDVFKWKHFWKVLAEQFGVECGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTK 333

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LE++GNWWF DI+L     +D+MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 334 LEDVGNWWFSDIVLGNECWLDTMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of Carg21791 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 2.6e-73
Identity = 124/175 (70.86%), Postives = 146/175 (83.43%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MN++GTLC YAAICKHEG  L+FPG K AW+ +S  SDADLIAE  IW AVDPYAKNEAF
Sbjct: 214 MNLVGTLCVYAAICKHEGAVLRFPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAF 273

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           N +NGDVFKWKHFWKVLAEQF +E   +EEG+++ L +LMKDK  VW++IV+EN L  T+
Sbjct: 274 NVSNGDVFKWKHFWKVLAEQFGVECGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTK 333

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LE++GNWWF DI+L     +D+MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 334 LEDVGNWWFSDIVLGNECWLDTMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of Carg21791 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 3.2e-71
Identity = 123/175 (70.29%), Postives = 143/175 (81.71%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MN++GTLC YAAICKHEG+ L+F G K+AW+ +S  SDADLIAE HIW AVDPYAKNEAF
Sbjct: 215 MNLVGTLCVYAAICKHEGKVLRFTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAF 274

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           N +NGDVFKWKHFWKVLAEQF +    +EEG  L L +LMK K  VWEEIV+EN L PT+
Sbjct: 275 NVSNGDVFKWKHFWKVLAEQFGVGCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTK 334

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           L+++G WWF D+IL     +DSMNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 335 LKDVGIWWFGDVILGNECFLDSMNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]

BLAST of Carg21791 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 4.2e-71
Identity = 128/176 (72.73%), Postives = 142/176 (80.68%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLC YAAICKHE  PLKFPGTK+AW  +SVASDADLIAE  IW AVDPYAKNEAF
Sbjct: 215 MNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAF 274

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLT-LVELMKDKSKVWEEIVKENQLQPT 120
           NC+NGD+FKWKH WKVLAEQF +E   F+E EK T LVE MKDK  VWEEIV+EN L  T
Sbjct: 275 NCSNGDLFKWKHLWKVLAEQFGVEYAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTT 334

Query: 121 RLEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           +LE +  WWF D+IL     +DSMNKSKEHG+LGFRN+KNS IS IDK+KA KIVP
Sbjct: 335 KLEGVATWWFADVILGGECLLDSMNKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390

[Close]

BLAST of Carg21791 vs. ExPASy TrEMBL
Match: A0A6J1IQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111477228 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.3e-99
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 219 MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. ExPASy TrEMBL
Match: A0A6J1F7T0 (uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC111442928 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.9e-99
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNIIGTLCAYAAIC+HEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 219 MNIIGTLCAYAAICRHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. ExPASy TrEMBL
Match: A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.2e-93
Identity = 157/175 (89.71%), Postives = 169/175 (96.57%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNI+GTLC YAAICKHEGRPLKFPGTK AWE+F  ASDADL+AEQHIWTAVDPY+KNEAF
Sbjct: 219 MNIVGTLCVYAAICKHEGRPLKFPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIE+YGFEEGE+LTLV+LMKDKS VW+EIVKENQLQPT+
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTK 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWW+VD+I S+SGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWYVDVIFSVSGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. ExPASy TrEMBL
Match: A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.2e-93
Identity = 157/175 (89.71%), Postives = 169/175 (96.57%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNI+GTLC YAAICKHEGRPLKFPGTK AWE+F  ASDADL+AEQHIWTAVDPY+KNEAF
Sbjct: 219 MNIVGTLCVYAAICKHEGRPLKFPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNNGDVFKWKHFWKVLAEQFNIE+YGFEEGE+LTLV+LMKDKS VW+EIVKENQLQPT+
Sbjct: 279 NCNNGDVFKWKHFWKVLAEQFNIEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTK 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEEIGNWW+VD+I S+SGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 339 LEEIGNWWYVDVIFSVSGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of Carg21791 vs. ExPASy TrEMBL
Match: A0A6J1J0N7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111480841 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 2.7e-89
Identity = 154/175 (88.00%), Postives = 164/175 (93.71%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNI+GTLC YAAICKHEGRPLKFPGTKSAWE +SV SDADLIAEQHIWTAVDPYAKNEAF
Sbjct: 219 MNIVGTLCTYAAICKHEGRPLKFPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAF 278

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NC+NGDVFKWKHFWKVLAEQFNIEDYGFEEGE+L L ELMK K++VWEEIVKENQLQPT+
Sbjct: 279 NCSNGDVFKWKHFWKVLAEQFNIEDYGFEEGERLPLGELMKGKNEVWEEIVKENQLQPTK 338

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LE++ NWW  DI LS SGNIDSMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 339 LEDVANWWHTDIALSASGNIDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of Carg21791 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-82
Identity = 136/175 (77.71%), Postives = 150/175 (85.71%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNI+GTLC YAAICKHEG PL FPG+K AWE F  ASDADLIAEQ IW AVDPYAKNEAF
Sbjct: 214 MNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAF 273

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNN D+FKWKH WK+LAEQF IE+YGFEEG+ L LVE+MK K +VWEE+VKENQLQ  +
Sbjct: 274 NCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKK 333

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEE+G WWF D+IL + G IDSMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 334 LEEVGVWWFADVILGVEGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of Carg21791 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-82
Identity = 136/175 (77.71%), Postives = 150/175 (85.71%), Query Frame = 0


Query: 1   MNIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAF 60
           MNI+GTLC YAAICKHEG PL FPG+K AWE F  ASDADLIAEQ IW AVDPYAKNEAF
Sbjct: 213 MNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAF 272

Query: 61  NCNNGDVFKWKHFWKVLAEQFNIEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTR 120
           NCNN D+FKWKH WK+LAEQF IE+YGFEEG+ L LVE+MK K +VWEE+VKENQLQ  +
Sbjct: 273 NCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKK 332

Query: 121 LEEIGNWWFVDIILSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 176
           LEE+G WWF D+IL + G IDSMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 333 LEEVGVWWFADVILGVEGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

[Close]

BLAST of Carg21791 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.0e-24
Identity = 61/185 (32.97%), Postives = 96/185 (51.89%), Query Frame = 0


Query: 2   NIIGTLCAYAAICKHEGRPLKFPGTKSAWEEFSV-ASDADLIAEQHIWTAVDPYA--KNE 61
           N +G+LC Y A+CK+   P  F GT+  WEE  +  SD++L+AEQHI+ A       K E
Sbjct: 207 NFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQHIFAATSGKVREKGE 266

Query: 62  AFNCNNGDVFKWKHFWKVLAEQFNIE-------DYGFEEGEKLTLVELMKDKSKVWEEIV 121
           AFN  NG  F WK  W  + ++  ++       D GF  G +      M ++  VW+EIV
Sbjct: 267 AFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGRE------MVERKHVWDEIV 326

Query: 122 KENQLQPTRLEEIGNWWFVDIILSISGN-IDSMNKSKEHGFLGFRNSKNSFISWIDKIKA 176
            + +L  T +E++ NW+F+D +       +    K    GF     + +S + WID ++ 
Sbjct: 327 VKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLDSVLYWIDVMRD 385

The following BLAST results are available for this feature:

BLAST of Carg21791 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024289.1	2.7e-99	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]
XP_022977039.1	2.7e-99	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]
KAG6591410.1	2.7e-99	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]
XP_022936254.1	5.9e-99	99.43	uncharacterized protein LOC111442928 [Cucurbita moschata]	[more]
XP_023535925.1	7.7e-99	98.86	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg21791 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9STX2	2.0e-81	77.71	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
A0A221J5W8	2.0e-73	70.86	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ...	[more]
A0A221J5X3	2.6e-73	70.86	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
Q6PQJ9	3.2e-71	70.29	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]
A0A1C9CX66	4.2e-71	72.73	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]

BLAST of Carg21791 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IQC1	1.3e-99	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1F7T0	2.9e-99	99.43	uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC1114429...	[more]
A0A5A7T3B3	5.2e-93	89.71	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BHA7	5.2e-93	89.71	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983...	[more]
A0A6J1J0N7	2.7e-89	88.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Carg21791 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24220.1	1.4e-82	77.71	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	1.4e-82	77.71	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G58750.1	1.0e-24	32.97	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 1..175 e-value: 4.8E-65 score: 221.8
None	No IPR available	PANTHER	PTHR32487:SF20	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE-LIKE	coord: 1..175
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..175
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 8..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21791-RA	Carg21791-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0008202	steroid metabolic process
biological_process	GO:0010051	xylem and phloem pattern formation
molecular_function	GO:0047568	3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function	GO:0035671	enone reductase activity
molecular_function	GO:0046983	protein dimerization activity