Carg21184 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21184
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr01: 11104296 .. 11109140 (-)
RNA-Seq ExpressionCarg21184
SyntenyCarg21184
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTTACCAAGATACAGCTTAGACAGAAAAGAGAGATTGAATGTGAATGGATTTGTGGCGGTGGCCATTGACAAAGACAAAGGCAGCCAAGGGGCTCTCAAATGGGCAATTGATCATTTGCTTCGCAGAGGCCAAACTGTTTTCCTCGTTCATGTCAAACTCAGATCCCTTTCTAATTCTTCCCTAAGTAAGTACTCTCTCTCTCTCTCTCAACTTTTTTATTTTTCATATCACAATTTTAGCTTCCATTAGGTTCGAAAGCTCGATCCGTTTCATTACAATTTTTTTTTAGATTCTCTATTCCTCGGAAATTTCATTTTTCATATATTTTTAAGAAGCAATGAGAGGTGTATACGGGTTGGATTGGGTTAATCTCATTGGTTTGTAAACATTAACCCCGAACTAAGTTTGTTTTTTGCAAAAGTTTAACTCAACCCAAATCAAAAATAAAAAACCTAACCAAACCCAACTTTTATAGTTTGAGTATGAATTTAGTAGTCCTGGTTGGTCGGATTATTGAGTCATTTTTAGGTTAGATATGAGAAAGAACATTTTATTATTAGGACGTATGTCTTAATGAGTCGAATATAAACTTTATTCATCAACGAATGATGAGAATATTTTGTTGAATTTAATCTTTCGAAAGTTTCTTTCTATTTCATTTCTTATGTATCAATTTCTTTAAGTTAAGCCTTTATCGTAAGTGAATATTAGGTTTACGTTCATCTAATACTCAAAACAGAACAAAACGTTTCATCGGCAACACAAGGTCGATTTGGTGTGGTGACTCACCTAACCCAAAATTATTTGTTTCTATAAAATATTTTACTTTGAATTCCCTTTGAGGAAAAAAAAAAAAACATAATTTTAGTGTATTTTTTGATTTTGCTCTCTAGAATGCTCATTTTCTATTAAATTTTATATTCTATATATAGATATATATATAATGAGGAAGCTCTTTTTGGATGCAGAGATGAAATCTGACCCTGAAGCTGGCGTTAGAGAATTATTCCTTCCTTTTCGTTGCTTTTGTACTCGAAAAGATGTAAGCTTTATTTATTTTATTTTATTTAATTGAAGATTCAATTTTTATCTATTATTTAGTATAATAATAATAAATTTTCTGAGTTTCAGATTCAATGCAAAGATGTTGTGTTGGAAGATACAGACATAGCTAAGGCCTTAACTGAGTATGCTGCAAATACAGCCATAGAGAACATTGTTGTGGGAGCTTCATCAAAATCTGGCTTTTTAAGGTATGTTTCTTTTCTTTTCTTTTCTTATTAGGTCAACATGTTTGAGAATCTTGATGATCCAAAAAACTCGACATGAATATTAAACTCGAACAATTGGATCGACTCGATAGGGTTTACCATGACCATGACGTTTTTCTCTTGGAGAATGTTTAATCATTTAGGCCGTTACCCATGACTCGATAGGCTTGGGCCGTTACAAATGACACCAGACTATGTGCCAACAAAGAGGTTGTTCCTCAAAGGGGGTGGACACGAGGCGGTGTGCCAGTAAGGACGCTGGGCCCGAAGGGGGGGTGGATTTGGTGGTGGTCCCACATCGATTGGAGAAACGAACAAGTGTCAGTGAGGACGCTGGGCCCTGAAGGGGGGTGGATTGTGATATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTTGAGAGGAAGCCCAAAAAAGACAATATCTGCAACCCATATGCTTGGGCCGTTACACATATTAAGTGTTCGACTTGAAAAACTCGATCAATGCAACCCAAAAGCTATGAGTATCAAACTCGAACAGTTCGTTGAGTTGAAAAGGTCGTTTTTATGGCATATTATGTATGAAGAAAGCATTGAAGTTTCTTGTAGATATCATTAACTTGATACAAGCTTCTTATGAAGCAGATTCAAGGCAACTGATGTTCCTGGCAATGTCTCAAAAGGAGCACCAGATTTTTGCAATGTGTTTGTCATTTCTAAAGGCAAAATTCAATCGATGCGTTCGGCATCTCGTCCCGCTCCGGTCGTGAATCCTCTACAGTCGCAATTAGTGCAACAAAGCAGCACAAAATCCATCAGGTCCGACTACCAATTTCCTCAGACTACATCTGCAAGAGGTTAACTTCTTTCAAACCAGTTTCCTTTTACCTTATATGCTTGTTGGTCGTCCCAATATTCTCCATGAAACTAACGTTAAATTTGTTAGCAGCCTCTTTGGATAAACGTCCCTTTGTTCTGCCACGTAGATCGCAGGACGAGGCTGATTTCATGAGGTGAGAGCGACCCTATACATCGTTCAAATAGACGTTAAAACTTCAGTTGTTAACTTAGTAAGCTATGACTAAATTGTATGACGTTTTAATATATTAGCTATTAATATTGATCGAAGTAGAACAAAACACTTTTACTCCGATTTTGTGTTAAACTACTACTAAATCCACGAGAAGCGTAAACTAGTTATGTTCTTTGTGATGTCCCACGTCTGTTGGGGAGGAGAACAAAATACCTTTTATAAGGGTGTGGAAACCTTTCCCTAGCAGACGCGTTTTAAAAAACTTTGAGGGAAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCGAGTGGTGGGCCTGAGCTGTTACAAATGGTATCAGAGCTAGACACTGGGTGATGTGCCAGCGAGGACGTTGGACCACGAAGGGAGGGGGGTTTGGTGGGGTCCCACATCGATTGGAGAAAGGAACGAGTGCCAGTGAGGACCCTGGACCCTGAAAGGGGGTGGATTGTGATGTCCCACATCAGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTCTCCCTAGAAGACGCGTTTTAAAAACCTTAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTAGGCTGTTACATTCTTACTTTGTTAGGCATACTTTGTTAGGCATACTGATGAGCAAAATGTCGTTGAAATCAGGTCACCCTTCACAAGGAAAGGATACAATTACAAACCGTCGTATGGAGATTCGTCTTTTCAAGAATCAGATATATCGTACGTTAGCTCAGGAAGACCGAGCATTGATCGTATATTTCCTTCAGTTTATGACAGCCTAGATATGGGAGGAAGAGCCACCCCACCACGGATGTCGAGTAGTACAGATCTTGACTTGACTCAGAGCTTCGAGTCTCTACCGATGGGACGGTTATCGATGGATATGAACTTCCCGTCCGAGTTCTCTTCGATCTCGCAAGATAGTGATAGATTGTCAATCTCATCACAGTCTTTGGTAAGTTTTGAGCCACATTATTGCCTATGGTTCCATTCATTTTATGCAATAAAGCTTTGTTTTATATGAACTTTTATATGATGGTGCAGGAAGATGTGGAGGCTGAGATGCGTAGGCTCAAGCTAGAACTCAAGCAAACCATGGAAATGTACAGCACAGCCTGCAAGGAAGCACTTACAGCAAAACAAAAGGTAAGACGAACCGATCGATTAACACGGATCGATATCGAGACCAGCTTTCAATGGCTGTTTCATGATATCTATGTTTCGAGTTGCAGGCGGTCGAGCTGCAACGCTGGAAATTAGAAGAAGAACAAAGAATAGAAGAGGCGAGATTGGCTGGGGAAGCTGCATTGGCGCTGGCCGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAGGCAGCAGAGGCAGCTAGGAGGATAGCAGAATTGGAAGCACAGAAAAGAATGAAGGCTGAAATGAAAGCTCACAAAGAAGCTGAAGAAAAGAAAAGGGCTATTGATGCATTGTCACAAAGCGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGAGGCAACGGAATTCTTTGCTGAATCTCGTAAGATCGGAGAAGGCGGTTATGGTCCAGTTTACAAGTGTGATCTTGATCATACTCCTGTTGCCATTAAGGTTTTACGTCCCGATGCAACTCAAGGACGATCGCAGTTTAAACAAGAGGTACGTTACCGAACAACTTCCATGTTTTAGAACGTCTAAGAGGTCTCATAACTTTATCTAATATAACGATTCATAGGTAGAAGTACTTAGCTGTATACGCCATCCAAACATGGTGCTCCTCCTTGGAGCGTGCCCCGAGTACGGCTGCTTGGTCTACGAGTTCATGGCTAATGGGAGCTTAGAAGATCGACTTCTACAACGAGGAAACACGCCTCCACTTTCGTGGCAGCTCAGGTTTCAAATTGCAGGTGAAATTGGAACAGCATTGTTGTTCCTACACCAAACAAAGCCAGAGCCACTCGTGCATCGTGATCTCAAACCAGCTAATATTTTGCTCGATCGTAACTTTGTTAGTAAGATCAGTGACGTCGGCTTAGCTAGGCTCGTACCTCCATCGGTTGCGGATAACGTAACACAGTACCGAATGACATCGACTGCTGGAACGTTTTGCTACATTGATCCCGAGTATCAGCAGACAGGCATGCTTGGTGTGAAATCAGACATATACTCATTAGGCATCATGTTTCTCCAGCTGATAACTTCTCGGCCGCCAATGGGATTGACTCACCATGTTGAACGAGCGATCGAGAATGATATGTTTGCAAATATTCTCGATCCAGCCGTTACCGATTGGCCTGTCGAAGAAGCGTTGTCGTTCGCCAAGATGTCGCTCAAGTGTGCCGAGTTAAGACGTAAAGATAGGCCTGATCTTGGAAAGGAAATCCTTCCCGAGCTTAACAGGTTGAGAACTATTGCAGAAGATTATATACACCCAACACTGATGGGAGACATAAGCAGCCCCACTCGCAGCAGCAGCCAAGTTTCTCTACCGTTG

mRNA sequence

ATGTGGTTACCAAGATACAGCTTAGACAGAAAAGAGAGATTGAATGTGAATGGATTTGTGGCGGTGGCCATTGACAAAGACAAAGGCAGCCAAGGGGCTCTCAAATGGGCAATTGATCATTTGCTTCGCAGAGGCCAAACTGTTTTCCTCGTTCATGTCAAACTCAGATCCCTTTCTAATTCTTCCCTAAAGATGAAATCTGACCCTGAAGCTGGCGTTAGAGAATTATTCCTTCCTTTTCGTTGCTTTTGTACTCGAAAAGATATTCAATGCAAAGATGTTGTGTTGGAAGATACAGACATAGCTAAGGCCTTAACTGAGTATGCTGCAAATACAGCCATAGAGAACATTGTTGTGGGAGCTTCATCAAAATCTGGCTTTTTAAGATTCAAGGCAACTGATGTTCCTGGCAATGTCTCAAAAGGAGCACCAGATTTTTGCAATGTGTTTGTCATTTCTAAAGGCAAAATTCAATCGATGCGTTCGGCATCTCGTCCCGCTCCGGTCGTGAATCCTCTACAGTCGCAATTAGTGCAACAAAGCAGCACAAAATCCATCAGGTCCGACTACCAATTTCCTCAGACTACATCTGCAAGAGCCTCTTTGGATAAACGTCCCTTTGTTCTGCCACGTAGATCGCAGGACGAGGCTGATTTCATGAGCTCAGGAAGACCGAGCATTGATCGTATATTTCCTTCAGTTTATGACAGCCTAGATATGGGAGGAAGAGCCACCCCACCACGGATGTCGAGTAGTACAGATCTTGACTTGACTCAGAGCTTCGAGTCTCTACCGATGGGACGGTTATCGATGGATATGAACTTCCCGTCCGAGTTCTCTTCGATCTCGCAAGATAGTGATAGATTGTCAATCTCATCACAGTCTTTGGAAGATGTGGAGGCTGAGATGCGTAGGCTCAAGCTAGAACTCAAGCAAACCATGGAAATGTACAGCACAGCCTGCAAGGAAGCACTTACAGCAAAACAAAAGGCGGTCGAGCTGCAACGCTGGAAATTAGAAGAAGAACAAAGAATAGAAGAGGCGAGATTGGCTGGGGAAGCTGCATTGGCGCTGGCCGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAGGCAGCAGAGGCAGCTAGGAGGATAGCAGAATTGGAAGCACAGAAAAGAATGAAGGCTGAAATGAAAGCTCACAAAGAAGCTGAAGAAAAGAAAAGGGCTATTGATGCATTGTCACAAAGCGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGAGGCAACGGAATTCTTTGCTGAATCTCGTAAGATCGGAGAAGGCGGTTATGGTCCAGTTTACAAGTGTGATCTTGATCATACTCCTGTTGCCATTAAGGTTTTACGTCCCGATGCAACTCAAGGACGATCGCAGTTTAAACAAGAGGTAGAAGTACTTAGCTGTATACGCCATCCAAACATGGTGCTCCTCCTTGGAGCGTGCCCCGAGTACGGCTGCTTGGTCTACGAGTTCATGGCTAATGGGAGCTTAGAAGATCGACTTCTACAACGAGGAAACACGCCTCCACTTTCGTGGCAGCTCAGGTTTCAAATTGCAGGTGAAATTGGAACAGCATTGTTGTTCCTACACCAAACAAAGCCAGAGCCACTCGTGCATCGTGATCTCAAACCAGCTAATATTTTGCTCGATCGTAACTTTGTTAGTAAGATCAGTGACGTCGGCTTAGCTAGGCTCGTACCTCCATCGGTTGCGGATAACGTAACACAGTACCGAATGACATCGACTGCTGGAACGTTTTGCTACATTGATCCCGAGTATCAGCAGACAGGCATGCTTGGTGTGAAATCAGACATATACTCATTAGGCATCATGTTTCTCCAGCTGATAACTTCTCGGCCGCCAATGGGATTGACTCACCATGTTGAACGAGCGATCGAGAATGATATGTTTGCAAATATTCTCGATCCAGCCGTTACCGATTGGCCTGTCGAAGAAGCGTTGTCGTTCGCCAAGATGTCGCTCAAGTGTGCCGAGTTAAGACGTAAAGATAGGCCTGATCTTGGAAAGGAAATCCTTCCCGAGCTTAACAGGTTGAGAACTATTGCAGAAGATTATATACACCCAACACTGATGGGAGACATAAGCAGCCCCACTCGCAGCAGCAGCCAAGTTTCTCTACCGTTG

Coding sequence (CDS)

ATGTGGTTACCAAGATACAGCTTAGACAGAAAAGAGAGATTGAATGTGAATGGATTTGTGGCGGTGGCCATTGACAAAGACAAAGGCAGCCAAGGGGCTCTCAAATGGGCAATTGATCATTTGCTTCGCAGAGGCCAAACTGTTTTCCTCGTTCATGTCAAACTCAGATCCCTTTCTAATTCTTCCCTAAAGATGAAATCTGACCCTGAAGCTGGCGTTAGAGAATTATTCCTTCCTTTTCGTTGCTTTTGTACTCGAAAAGATATTCAATGCAAAGATGTTGTGTTGGAAGATACAGACATAGCTAAGGCCTTAACTGAGTATGCTGCAAATACAGCCATAGAGAACATTGTTGTGGGAGCTTCATCAAAATCTGGCTTTTTAAGATTCAAGGCAACTGATGTTCCTGGCAATGTCTCAAAAGGAGCACCAGATTTTTGCAATGTGTTTGTCATTTCTAAAGGCAAAATTCAATCGATGCGTTCGGCATCTCGTCCCGCTCCGGTCGTGAATCCTCTACAGTCGCAATTAGTGCAACAAAGCAGCACAAAATCCATCAGGTCCGACTACCAATTTCCTCAGACTACATCTGCAAGAGCCTCTTTGGATAAACGTCCCTTTGTTCTGCCACGTAGATCGCAGGACGAGGCTGATTTCATGAGCTCAGGAAGACCGAGCATTGATCGTATATTTCCTTCAGTTTATGACAGCCTAGATATGGGAGGAAGAGCCACCCCACCACGGATGTCGAGTAGTACAGATCTTGACTTGACTCAGAGCTTCGAGTCTCTACCGATGGGACGGTTATCGATGGATATGAACTTCCCGTCCGAGTTCTCTTCGATCTCGCAAGATAGTGATAGATTGTCAATCTCATCACAGTCTTTGGAAGATGTGGAGGCTGAGATGCGTAGGCTCAAGCTAGAACTCAAGCAAACCATGGAAATGTACAGCACAGCCTGCAAGGAAGCACTTACAGCAAAACAAAAGGCGGTCGAGCTGCAACGCTGGAAATTAGAAGAAGAACAAAGAATAGAAGAGGCGAGATTGGCTGGGGAAGCTGCATTGGCGCTGGCCGAACAGGAGAAGGCGAAGTCGAAAGCAGCCATCGAGGCAGCAGAGGCAGCTAGGAGGATAGCAGAATTGGAAGCACAGAAAAGAATGAAGGCTGAAATGAAAGCTCACAAAGAAGCTGAAGAAAAGAAAAGGGCTATTGATGCATTGTCACAAAGCGATATCAGGTATAGAAAGTATACGATCGAGGAGATCGAAGAGGCAACGGAATTCTTTGCTGAATCTCGTAAGATCGGAGAAGGCGGTTATGGTCCAGTTTACAAGTGTGATCTTGATCATACTCCTGTTGCCATTAAGGTTTTACGTCCCGATGCAACTCAAGGACGATCGCAGTTTAAACAAGAGGTAGAAGTACTTAGCTGTATACGCCATCCAAACATGGTGCTCCTCCTTGGAGCGTGCCCCGAGTACGGCTGCTTGGTCTACGAGTTCATGGCTAATGGGAGCTTAGAAGATCGACTTCTACAACGAGGAAACACGCCTCCACTTTCGTGGCAGCTCAGGTTTCAAATTGCAGGTGAAATTGGAACAGCATTGTTGTTCCTACACCAAACAAAGCCAGAGCCACTCGTGCATCGTGATCTCAAACCAGCTAATATTTTGCTCGATCGTAACTTTGTTAGTAAGATCAGTGACGTCGGCTTAGCTAGGCTCGTACCTCCATCGGTTGCGGATAACGTAACACAGTACCGAATGACATCGACTGCTGGAACGTTTTGCTACATTGATCCCGAGTATCAGCAGACAGGCATGCTTGGTGTGAAATCAGACATATACTCATTAGGCATCATGTTTCTCCAGCTGATAACTTCTCGGCCGCCAATGGGATTGACTCACCATGTTGAACGAGCGATCGAGAATGATATGTTTGCAAATATTCTCGATCCAGCCGTTACCGATTGGCCTGTCGAAGAAGCGTTGTCGTTCGCCAAGATGTCGCTCAAGTGTGCCGAGTTAAGACGTAAAGATAGGCCTGATCTTGGAAAGGAAATCCTTCCCGAGCTTAACAGGTTGAGAACTATTGCAGAAGATTATATACACCCAACACTGATGGGAGACATAAGCAGCCCCACTCGCAGCAGCAGCCAAGTTTCTCTACCGTTG

Protein sequence

MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSNSSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL
Homology
BLAST of Carg21184 vs. NCBI nr
Match: KAG7037575.1 (U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 726/726 (100.00%), Postives = 726/726 (100.00%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDM 240
           SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDM
Sbjct: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDM 240

Query: 241 GGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVE 300
           GGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVE
Sbjct: 241 GGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVE 300

Query: 301 AEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAE 360
           AEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAE
Sbjct: 301 AEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAE 360

Query: 361 QEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTI 420
           QEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTI
Sbjct: 361 QEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTI 420

Query: 421 EEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCI 480
           EEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCI
Sbjct: 421 EEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCI 480

Query: 481 RHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFL 540
           RHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFL
Sbjct: 481 RHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFL 540

Query: 541 HQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYI 600
           HQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYI
Sbjct: 541 HQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYI 600

Query: 601 DPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWP 660
           DPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWP
Sbjct: 601 DPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWP 660

Query: 661 VEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSS 720
           VEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSS
Sbjct: 661 VEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSS 720

Query: 721 QVSLPL 727
           QVSLPL
Sbjct: 721 QVSLPL 726

BLAST of Carg21184 vs. NCBI nr
Match: XP_022941502.1 (U-box domain-containing protein 52-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1377.5 bits (3564), Expect = 0.0e+00
Identity = 721/754 (95.62%), Postives = 723/754 (95.89%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                    
Sbjct: 181 SSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           KAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 721 LNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 753

BLAST of Carg21184 vs. NCBI nr
Match: XP_022941501.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 721/755 (95.50%), Postives = 723/755 (95.76%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           QSSTKSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                   
Sbjct: 181 QSSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           MKAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 754

BLAST of Carg21184 vs. NCBI nr
Match: XP_023524077.1 (U-box domain-containing protein 52-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1368.6 bits (3541), Expect = 0.0e+00
Identity = 715/754 (94.83%), Postives = 721/754 (95.62%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSIRSDYQFPQTTSARASLDKRPFVLPR SQDEADFM                    
Sbjct: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRGSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALA+QEK KSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAKQEKTKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY+CDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYRCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVE+AIENDMFANILDPA+TDWP+EEALSFAKMSLKCAELRRKDRPDLGKEILPE
Sbjct: 661 MGLTHHVEQAIENDMFANILDPAITDWPIEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDYIHPTLMGDI SPTRSSSQVSL L
Sbjct: 721 LNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLQL 753

BLAST of Carg21184 vs. NCBI nr
Match: XP_023524076.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 715/755 (94.70%), Postives = 721/755 (95.50%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           QSSTKSIRSDYQFPQTTSARASLDKRPFVLPR SQDEADFM                   
Sbjct: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRGSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALA+QEK KSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAKQEKTKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY+CDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYRCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVE+AIENDMFANILDPA+TDWP+EEALSFAKMSLKCAELRRKDRPDLGKEILP
Sbjct: 661 PMGLTHHVEQAIENDMFANILDPAITDWPIEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLRTIAEDYIHPTLMGDI SPTRSSSQVSL L
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLQL 754

BLAST of Carg21184 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 5.5e-117
Identity = 283/735 (38.50%), Postives = 392/735 (53.33%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVF-LVHVKLRSLSNSSLKMKSDPEAGVR---- 79
           V VA+     S+  + WAI+     G   F L+H+     S  +    + P + VR    
Sbjct: 22  VVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVV 81

Query: 80  ------------ELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGAS 139
                       E+  P+     R+ +  + +V+E  ++A A+ E     +I+ IV+G S
Sbjct: 82  TAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGS 141

Query: 140 SKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMR-SASRPAPVVNPLQSQLVQQS 199
           S+S F R    D+   +S   P+FC V+V+SKGK+  +R S S     +    S+    S
Sbjct: 142 SRSFFSR--KADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSS 201

Query: 200 STKSIRSDYQFPQTTSARASLDKRPFVLP-RRSQ--------------------DEADFM 259
           S  S  +       +SA  S   RP  LP RR Q                    DE   M
Sbjct: 202 SGSSGPTSDSSDVMSSAHDS-QSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCM 261

Query: 260 SSGRPSIDRIFPSVYDSLDMGGRATPPR----------MSSSTDLDLTQSFESLPMGRLS 319
           S        +      S D   R TP R           SSS++ +            + 
Sbjct: 262 SLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMG 321

Query: 320 MDMNFPSEFSSISQDSDRLSISSQSLEDVEA--EMRRLKLELKQTMEMYSTACKEALTAK 379
           +D          S  SD LS  S +   V    E+ +L+ EL+   EMY+ A  E   A 
Sbjct: 322 VDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDAS 381

Query: 380 QKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRM 439
           +K  EL + +LEE  ++EE +L    A  LAE+EK   + A   AE+ R  AE E  +R 
Sbjct: 382 RKLGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRR 441

Query: 440 KAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCD 499
           +AE K+ ++ +EK++    L    ++Y+ +  EEI  AT  F+E  KIG G YG VYKC+
Sbjct: 442 EAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCN 501

Query: 500 LDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSL 559
           L HT   +KVL+    Q   QF+QE+E+LS IRHP++VLLLGACPE G LVYE+M NGSL
Sbjct: 502 LHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSL 561

Query: 560 EDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKI 619
           EDRL Q  N+PPL W  RF+IA E+  AL+FLH++KP+P++HRDLKPANILLD NFVSK+
Sbjct: 562 EDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKV 621

Query: 620 SDVGLARLVP-PSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLI 679
            DVGL+ +V    ++   T Y+ TS  GT CYIDPEYQ+TG +  KSDIYS G++ LQL+
Sbjct: 622 GDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLL 681

Query: 680 TSRPPMGLTHHVERAIE-NDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLG 702
           T++P + LTH VE A++ ND F  ILD    +WP+EE    A ++L C ELR KDRPDL 
Sbjct: 682 TAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLK 741

BLAST of Carg21184 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.0e-115
Identity = 279/764 (36.52%), Postives = 408/764 (53.40%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVF-LVHVK------------------LRSLSN 79
           VAVAI+  K S+  + WA++  +  G T F L++V+                  LR    
Sbjct: 24  VAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDVV 83

Query: 80  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 139
           S+ K + D  A   E+  P++    R+ +Q + ++L+  + A A+ E  A T +  +V+G
Sbjct: 84  SAYKQELDWSA--NEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIG 143

Query: 140 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 199
            S +  F R    D+   ++   P FC V+VISKGK+ S+R +               + 
Sbjct: 144 MSLRGFFSR--KIDMSSLIATAVPRFCTVYVISKGKLASVRPS---------------ES 203

Query: 200 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDM 259
            ++ SIR    F +++S   S D     LP   QD    +S  +  +    P++  S+  
Sbjct: 204 DASGSIR----FERSSSTSGSTDSPR--LPPEYQDFLSAVSEAQSRVSPFSPALKHSMGS 263

Query: 260 GGRATPPRMSSSTDLD--------------------LTQSFE-SLPMGR----------- 319
              A     SS TD +                      +SF  S PMG            
Sbjct: 264 NAVAQMDTSSSGTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSK 323

Query: 320 ---------------------LSMDMN--FPSEFSSISQDSDRLS---ISSQSLED---- 379
                                ++MD     P  +S +S  +  +S   +S  S+ D    
Sbjct: 324 WRDHEDRREMRSSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVN 383

Query: 380 VEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALAL 439
           +  E+ +L+ ELK   EMY+ A  E + A +K  EL + + EE +++ E +   E A   
Sbjct: 384 LNFEIEKLRAELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDT 443

Query: 440 AEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKY 499
           A +EK + + A++ AE  + +   EA  R +AE KA ++A EK +   +L    ++Y+ Y
Sbjct: 444 ASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHY 503

Query: 500 TIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLS 559
           T EEI  AT  FAE+ KIG G YG VYKC+L HT  A+KVL    TQ   QF QE+E+LS
Sbjct: 504 TWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILS 563

Query: 560 CIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALL 619
            IRHP++VLLLGACPE GCLVYE+M NGSL+DRL+   +TPP+ W  RF+IA E+ +AL+
Sbjct: 564 KIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALV 623

Query: 620 FLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFC 679
           FLH++KP P++HRDLKP NILLD NFVSK+ DVGL+ +V      + T ++ TS  GT C
Sbjct: 624 FLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLC 683

Query: 680 YIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDM-FANILDPAVT 702
           YIDPEYQ+TG++  KSD+YSLG++ LQLIT++P + +TH VE AI +D  F  ILD    
Sbjct: 684 YIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAG 743

BLAST of Carg21184 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 414.1 bits (1063), Expect = 3.3e-114
Identity = 279/715 (39.02%), Postives = 400/715 (55.94%), Query Frame = 0

Query: 20  VAVAI-DKDKGSQGALKWAIDHLLRRGQTVF-LVHVKLRSLSNSSLK--------MKSDP 79
           VAVAI   +  ++G ++WA+     +   VF L+HV+ R  ++ S           K D 
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKDV 67

Query: 80  EAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGF-L 139
           +   RE+ LP R     +++Q   +VLE  DIA A+++   +  I  +V+GASS   F  
Sbjct: 68  DRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSW 127

Query: 140 RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRP-----APVVNPLQSQLVQQSST 199
           + K +++   ++   P FC+V VISKGK+ ++R +        A   +  +      S T
Sbjct: 128 KLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSSDSHSGT 187

Query: 200 KSIRSDYQFPQT------TSARASLDKRPFVLPRRSQDEADFMSSGRP-SIDRIFPSVYD 259
            S  S +QF  T        A  +++++      +  +E       R  S+D       D
Sbjct: 188 VSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRAGSLD------VD 247

Query: 260 SLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSL 319
              +  +    R SSS    +      +   R S       E SS S  SD  S SSQ  
Sbjct: 248 ESKLLNQKGFYRTSSS---GIGYGGSDISSWRSSQ----MEEASSSSTYSDPTSSSSQIH 307

Query: 320 EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAAL 379
           +D E E  +LK+EL+    MY+ A  E + A +K  +L + + EE  R++   +  E A 
Sbjct: 308 KDFELE--KLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEAD 367

Query: 380 ALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDI--- 439
            + E E+ + + A   AE  R   E E ++R++AE +A +  +EK+R  DAL    +   
Sbjct: 368 EVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPLQRQ 427

Query: 440 RYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQE 499
           +Y K+  EEI EAT  F++  KIG GGYG VY+C+L HT VA+KVL  D +    QF QE
Sbjct: 428 QYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQE 487

Query: 500 VEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQR------GNTPPLSWQLRF 559
           +E+LS IRHP+++LLLGACPE G LVYE+M NGSLE+RL++R         PPL W  RF
Sbjct: 488 LEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERF 547

Query: 560 QIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQ 619
           +IA EI +AL FLH  +P P+VHRDLKPANILLDRN VSKI DVGL+++V    +   T 
Sbjct: 548 RIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTV 607

Query: 620 YRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAI--EN 679
           +  T   GTF YIDPEYQ+TG++  +SDIY+ GI+ LQL+T+R  MGL H +E+A+  + 
Sbjct: 608 FNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQT 667

Query: 680 DMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIA 701
             F  ILD    DWPV+EA     + L+CAE+R++DRPDLGKEILP L RL+ +A
Sbjct: 668 GKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVA 707

BLAST of Carg21184 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.1e-106
Identity = 268/707 (37.91%), Postives = 373/707 (52.76%), Query Frame = 0

Query: 19  FVAVAI-----DK--DKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSNSSLKMKSDPEA 78
           FVAVA+     DK    GS+ A++WA+D+LL +     ++HV           + S P  
Sbjct: 23  FVAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHV--------IPTITSIPTP 82

Query: 79  GVRELFLPFRCFCTRKD------IQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKS 138
            +  L        T  D      ++   V +   D+ K   E      ++      S+K 
Sbjct: 83  NILILMFTRMWVVTAGDRLPVEEVEESVVEMYVRDVKKEY-ETVFVPFLKMCKSTRSTKR 142

Query: 139 GF--LRFKATDVPGNVSKGAPDFCNVFVISKGKI--QSMRSASRPAPVVNPLQSQLVQQ- 198
            F   R K T VP  V + AP+ C V+++ K +I  +SM       P  +P  +      
Sbjct: 143 YFRSRRTKGTGVPLTVLRYAPETCEVYIVCKDRITTKSMDPLINREPCTSPHAAATAHDF 202

Query: 199 -----SSTKSIRSDYQFPQTTSARASLD--KRPFVLPRRSQDEADFMSSGRPSIDRIFPS 258
                +S  ++RS    P     R S +   R     R  + EA  ++  +P   +    
Sbjct: 203 LRDWAASFHTLRS----PTLPDPRQSTEAGTRRSASARELRFEALSLTCNKPKTPQ---- 262

Query: 259 VYDSLDMGGRATPPRMSSSTDLDLTQ----SFESLPMGRLSMDMNFPSEFSSISQDSDRL 318
              S       TP         D+ Q     F+       S   N  SE     +     
Sbjct: 263 ---SSKASSATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIVSEHRDSDRSPPET 322

Query: 319 SISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEAR 378
           S  S+ +E +E E+ RLK EL+ T+  Y  AC+E  + + K   L    L E +R+  A 
Sbjct: 323 SRKSKKVE-IEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRVNNAV 382

Query: 379 LAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALS 438
              E     A  EK +   A++  E A+ +   E  +R  AE+ A +   EKK+ ID L 
Sbjct: 383 EKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLL 442

Query: 439 QSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQ 498
            +D RYRKYTIEEI  ATE F+  + IGEGGYG VY+C LD TP A+KV+R D  + + +
Sbjct: 443 GTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQE 502

Query: 499 FKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQI 558
           F +EVEVLS +RHP++VLLLGACPE GCLVYE++ NGSLE+ +  R N PPL W +RF++
Sbjct: 503 FLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRV 562

Query: 559 AGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYR 618
             E+   L FLH +KPEP+VHRDLKP NILL+RN+VSKI+DVGLA+LV     DNVT YR
Sbjct: 563 IFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYR 622

Query: 619 MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFA 678
            +  AGT  YIDPEY +TG +  KSD+Y+ GI+ LQL+T+R P G+   VE A++     
Sbjct: 623 NSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKKGTLT 682

Query: 679 NILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNRL 697
            +LD +VTDWP+ E    A++ LKCAE R +DRPDL  E++P L RL
Sbjct: 683 EMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVLKRL 708

BLAST of Carg21184 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.5e-102
Identity = 262/728 (35.99%), Postives = 385/728 (52.88%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVFL---VHVKLRSLSNSSLKMKSDPE------ 79
           VA+AI     S+  +KWA++         F    +H K+ +L  +S  + S  E      
Sbjct: 34  VALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISEELEEVA 93

Query: 80  AGVRE---------LFLPFRCFCTRKDIQCKDV--------------VLEDTDIAKALTE 139
           A  R+         L  PF+  C RK ++  +               VLE   +A A+T+
Sbjct: 94  AAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSVAVAITK 153

Query: 140 YAANTAIENIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSM-RSASRP 199
                 I N+++G SS++   R    D+  ++S    + C V+V+S G +  + +  S  
Sbjct: 154 EVNQHLISNLIIGRSSQAASSR--NYDITASISASVSNLCTVYVVSNGGVHILAKDTSDT 213

Query: 200 APVVNPLQSQLVQQSSTKSIRSDYQFPQTTSARAS-----LDKRPFVLPRRSQDEADFMS 259
                 ++S   + SS+ S  S       ++A  S      +KR   LP   +  +  M 
Sbjct: 214 ERNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPTIVRGVSVPME 273

Query: 260 SGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLD-LTQSFESLPMGRLSMDMNFPSEFS 319
           +     D       D+ +   + + P  S S   +   + F+       SM  NF  E+ 
Sbjct: 274 TSSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKDAMSSMSSNF--EYG 333

Query: 320 SISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLE 379
           ++          +Q   D   E+ +L+ EL+   EMY+ A  E L A +K  EL   K E
Sbjct: 334 NVVTPLGHYFTDNQ---DTLNEISKLRAELRHAHEMYAVAQVETLDASRKLNEL---KFE 393

Query: 380 EEQRIEEARLAGEAALALAEQE-KAKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAE 439
           E              L L E E K  +K   E  E  RR  E EA +R +AEMKA  EA+
Sbjct: 394 E--------------LTLLEHETKGIAKKETEKFEQKRR-EEREAAQRREAEMKATHEAK 453

Query: 440 EKKRAID-ALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKV 499
           EK++  + +L    ++Y+++T EEI  AT  F+E  KIG G YG VYKC+L HT  A+KV
Sbjct: 454 EKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKV 513

Query: 500 LRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNT 559
           L    +    QF QE+E+LS IRHP++VLLLGACP++G LVYE+M NGSLEDRL Q  ++
Sbjct: 514 LHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDS 573

Query: 560 PPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVP 619
            P+ W +R +IA E+ +AL+FLH++KP P++HRDLKPANILL+ NFVSK+ DVGL+ ++ 
Sbjct: 574 QPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQ 633

Query: 620 PS--VADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLT 679
            +  ++   T Y+ TS  GT CYIDPEYQ+TG +  KSD+Y+ G++ LQL+T +  M LT
Sbjct: 634 AADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALT 693

Query: 680 HHVERAIEN---DMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPEL 702
           + VE A+EN   D    ILD    +WP+EE    A ++L+C ELR KDRPDL  +ILP L
Sbjct: 694 YTVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVL 736

BLAST of Carg21184 vs. ExPASy TrEMBL
Match: A0A6J1FML4 (U-box domain-containing protein 52-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446783 PE=4 SV=1)

HSP 1 Score: 1377.5 bits (3564), Expect = 0.0e+00
Identity = 721/754 (95.62%), Postives = 723/754 (95.89%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                    
Sbjct: 181 SSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           KAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 721 LNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 753

BLAST of Carg21184 vs. ExPASy TrEMBL
Match: A0A6J1FLA1 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446783 PE=4 SV=1)

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 721/755 (95.50%), Postives = 723/755 (95.76%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           QSSTKSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                   
Sbjct: 181 QSSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           MKAHKEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 721 ELNRLRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 754

BLAST of Carg21184 vs. ExPASy TrEMBL
Match: A0A6J1IYC9 (U-box domain-containing protein 35-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481038 PE=4 SV=1)

HSP 1 Score: 1357.4 bits (3512), Expect = 0.0e+00
Identity = 709/754 (94.03%), Postives = 717/754 (95.09%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQ 180
           ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQ
Sbjct: 121 ASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQ 180

Query: 181 SSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM-------------------- 240
           SSTKSIRSDYQFPQTTSAR SLDKRP  LPRRSQDE DFM                    
Sbjct: 181 SSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSSF 240

Query: 241 --------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMD 300
                   SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSMD
Sbjct: 241 QESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSMD 300

Query: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360
           MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV
Sbjct: 301 MNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAV 360

Query: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420
           ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM
Sbjct: 361 ELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEM 420

Query: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHT 480
           KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDHT
Sbjct: 421 KAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDHT 480

Query: 481 PVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540
           PVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL
Sbjct: 481 PVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRL 540

Query: 541 LQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600
           L+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG
Sbjct: 541 LRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVG 600

Query: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660
           LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP
Sbjct: 601 LARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPP 660

Query: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPE 720
           MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LPE
Sbjct: 661 MGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLPE 720

Query: 721 LNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LNRLRTIAEDY+HPTLMGDI SPTRSSSQVS+PL
Sbjct: 721 LNRLRTIAEDYMHPTLMGDI-SPTRSSSQVSIPL 753

BLAST of Carg21184 vs. ExPASy TrEMBL
Match: A0A6J1J1M6 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481038 PE=4 SV=1)

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 709/755 (93.91%), Postives = 717/755 (94.97%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRS SN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 61  SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120
           SSLKMK DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG
Sbjct: 61  SSLKMKCDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVG 120

Query: 121 ASSKSGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQ 180
           ASSKSGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQ
Sbjct: 121 ASSKSGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQ 180

Query: 181 QSSTKSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM------------------- 240
           QSSTKSIRSDYQFPQTTSAR SLDKRP  LPRRSQDE DFM                   
Sbjct: 181 QSSTKSIRSDYQFPQTTSARDSLDKRPIDLPRRSQDETDFMRSPFTRKGYNYKPSYGDSS 240

Query: 241 ---------SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSM 300
                    SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSST+LDLT+SFESLPMGRLSM
Sbjct: 241 FQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTNLDLTRSFESLPMGRLSM 300

Query: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360
           DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA
Sbjct: 301 DMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 360

Query: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420
           VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE
Sbjct: 361 VELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAE 420

Query: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDH 480
           MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY CDLDH
Sbjct: 421 MKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYNCDLDH 480

Query: 481 TPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540
           TPVAIKVLRPDATQGR QFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR
Sbjct: 481 TPVAIKVLRPDATQGRLQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDR 540

Query: 541 LLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600
           LL+RGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV
Sbjct: 541 LLRRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDV 600

Query: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660
           GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP
Sbjct: 601 GLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRP 660

Query: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILP 720
           PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAK+SLKCAELRRKDRPDLGKE+LP
Sbjct: 661 PMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKLSLKCAELRRKDRPDLGKEVLP 720

Query: 721 ELNRLRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           ELNRLRTIAEDY+HPTLMGDI SPTRSSSQVS+PL
Sbjct: 721 ELNRLRTIAEDYMHPTLMGDI-SPTRSSSQVSIPL 754

BLAST of Carg21184 vs. ExPASy TrEMBL
Match: A0A6J1FSA4 (U-box domain-containing protein 52-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111446783 PE=4 SV=1)

HSP 1 Score: 1247.3 bits (3226), Expect = 0.0e+00
Identity = 658/691 (95.22%), Postives = 660/691 (95.51%), Query Frame = 0

Query: 65  MKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSK 124
           MKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSK
Sbjct: 1   MKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSK 60

Query: 125 SGFL-RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQLVQQSST 184
           SGFL RFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPL+SQLVQQSST
Sbjct: 61  SGFLSRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLVQQSST 120

Query: 185 KSIRSDYQFPQTTSARASLDKRPFVLPRRSQDEADFM----------------------- 244
           KSI SDYQFPQTTSARASLDKRPFVLPRRSQDEADFM                       
Sbjct: 121 KSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDSSFQES 180

Query: 245 -----SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNF 304
                SSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNF
Sbjct: 181 DISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLSMDMNF 240

Query: 305 PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ 364
           PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ
Sbjct: 241 PSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAVELQ 300

Query: 365 RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH 424
           RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH
Sbjct: 301 RWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKAEMKAH 360

Query: 425 KEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVA 484
           KEAEEKKRAIDALSQ+DIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVA
Sbjct: 361 KEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLDHTPVA 420

Query: 485 IKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQR 544
           IKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQR
Sbjct: 421 IKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLEDRLLQR 480

Query: 545 GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLAR 604
           GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLAR
Sbjct: 481 GNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLAR 540

Query: 605 LVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGL 664
           LVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGL
Sbjct: 541 LVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGL 600

Query: 665 THHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNR 724
           THHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNR
Sbjct: 601 THHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDLGKEILPELNR 660

Query: 725 LRTIAEDYIHPTLMGDISSPTRSSSQVSLPL 727
           LRTIAEDYIHPTLMGDI SPTRSSSQVSLPL
Sbjct: 661 LRTIAEDYIHPTLMGDI-SPTRSSSQVSLPL 690

BLAST of Carg21184 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 940.3 bits (2429), Expect = 9.5e-274
Identity = 508/783 (64.88%), Postives = 607/783 (77.52%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLR---- 60
           MW+P+ +    +    +G VAVAIDKDK SQ ALKWA+D+LL+RGQ+V LVHVKLR    
Sbjct: 1   MWMPKNNNVGVKEGGSSGVVAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRPSPL 60

Query: 61  ----SLSNSSLKMKS-------DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKAL 120
               SL  SS K+         DPE   +E+FLPFRCFCTRKDIQC+DV+LE++D+AKAL
Sbjct: 61  NNSASLHASSAKLSQDSSLVCRDPEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKAL 120

Query: 121 TEYAANTAIENIVVGASSKSGFLRF-KATDVPGNVSKGAPDFCNVFVISKGKIQSMRSAS 180
            EY    AIE +VVG+SSK GFLRF K TD+PG+++K APDFC V++ISKGKIQ+MRSAS
Sbjct: 121 VEYVNQAAIEVLVVGSSSKGGFLRFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSAS 180

Query: 181 RPAPVVNPLQS----------QLVQQSSTKSIRSDYQFPQTTSARASLDKRPFVL---PR 240
           R AP+  PL+S          Q +  +S  S+R+D +  ++   R+  D++   +    R
Sbjct: 181 RSAPMTAPLRSSVQPPSLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQR 240

Query: 241 RSQD---------------------------EADFMSSGRPSIDRIFPSVYDSLDMGGRA 300
           RS D                           +  F+SSGRPSIDRIFPS+YD+ D     
Sbjct: 241 RSIDDQSESFRSPFTRRGNGRSYGDLTVPESDISFISSGRPSIDRIFPSLYDNNDPS--R 300

Query: 301 TPPRMSSSTDLDLTQSF-ESLPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEM 360
           TPPR+S+ +D+D + S  +S   GR S+DMN P++F     +S+R S +SQS++DVEAEM
Sbjct: 301 TPPRLSNFSDMDYSSSLDQSSNYGRRSVDMNSPTDF-----ESERFSSASQSIDDVEAEM 360

Query: 361 RRLKLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEK 420
           RRLKLELKQTMEMYSTACKEALTAKQKA ELQRWKLEEE+++EEAR A EAALA+AE+EK
Sbjct: 361 RRLKLELKQTMEMYSTACKEALTAKQKATELQRWKLEEERKLEEARNAEEAALAIAEKEK 420

Query: 421 AKSKAAIEAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEI 480
           AKSKAA+EAAEAA+RIAELEA+KR+ AEMKA KE+EEK +A+ AL+ SD+RYRKY+IE+I
Sbjct: 421 AKSKAAMEAAEAAQRIAELEAKKRVNAEMKALKESEEKTKALTALANSDVRYRKYSIEDI 480

Query: 481 EEATEFFAESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHP 540
           E ATEFFAE  KIGEGGYGPVYKC LDHTPVA+KVLRPDA QGRSQF+QEVEVLSCIRHP
Sbjct: 481 ELATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSCIRHP 540

Query: 541 NMVLLLGACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQT 600
           NMVLLLGACPE GCLVYEFMANGSLEDRL + GN+PPLSWQ+RF+IA EIGT LLFLHQ 
Sbjct: 541 NMVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLFLHQA 600

Query: 601 KPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPE 660
           KPEPLVHRDLKP NILLDRNFVSKISDVGLARLVPP+VAD VTQYRMTSTAGTFCYIDPE
Sbjct: 601 KPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCYIDPE 660

Query: 661 YQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEE 720
           YQQTGMLGVKSDIYSLGIMFLQLIT++PPMGLTH+VERA+E     ++LDP V+DWP+E+
Sbjct: 661 YQQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHYVERALEKGTLVDLLDPVVSDWPMED 720

Query: 721 ALSFAKMSLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVS 727
              FAK++LKCAELRRKDRPDL K ILPELNRLRT+A++  H  ++G+   P+ + SQ S
Sbjct: 721 TEEFAKLALKCAELRRKDRPDLAKVILPELNRLRTLADESSHSVVVGNSPLPSPTGSQSS 776

BLAST of Carg21184 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 884.4 bits (2284), Expect = 6.2e-257
Identity = 481/762 (63.12%), Postives = 584/762 (76.64%), Query Frame = 0

Query: 9   DRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLS---NSSLKM 68
           DR     V+G VAVAID+DK SQGALKWA+D+LL++GQTV LVHVK R+ S   N S+  
Sbjct: 7   DRDGGFGVDGLVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINS 66

Query: 69  KS---------------DPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAA 128
            S               +PE   ++LFLPFRC C+RKDIQCKDV+LE++D+A+AL EYA 
Sbjct: 67  NSSKTSQINGDSSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYAN 126

Query: 129 NTAIENIVVGASSKSGFLRF-KATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPV 188
              IE +VVG+SSK GFLRF K  D+PG ++K APDFC V+ I+KGK+ + ++ASR AP 
Sbjct: 127 QVMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPS 186

Query: 189 VNPLQSQLVQQ---------SSTKSIRSDYQF----------PQTTSARASLDKRPFVLP 248
           V+PL+ QL Q          S+T + R++ Q            Q+ S R   +KR   L 
Sbjct: 187 VSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQHRRSLDDQSDSFRPPYNKRG--LT 246

Query: 249 RRS-------QDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFES 308
            RS         E  F SSGRPSI+R  PS+YD+ D     TPPR+S+ +D+D   SFES
Sbjct: 247 GRSYGELSIPDSEISFNSSGRPSIERNSPSLYDNSD--PNRTPPRLSNFSDVDYC-SFES 306

Query: 309 LPMGRLSMDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKE 368
           +  GR SMD++ P+ FS+ S +++R S +SQ  +DVEAEMRRLKLELKQTMEMYSTACKE
Sbjct: 307 MTFGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKE 366

Query: 369 ALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELE 428
           ALTAK KA ELQRWKL EE++ EEA+LA EAALA+AE+EKAKSKAA+EAAEAA+RIA++E
Sbjct: 367 ALTAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAMEAAEAAQRIADIE 426

Query: 429 AQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGP 488
           ++KR+ AE KA KE+E + +A++AL++ D+RYRKY+IEEIE+ATEFF +  KIGEG YGP
Sbjct: 427 SRKRVDAETKALKESEARTKAVNALAK-DVRYRKYSIEEIEDATEFFDDKYKIGEGSYGP 486

Query: 489 VYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFM 548
           VYKC LDHTPVA+K LRPDA QGRSQF++EVEVL  IRHPNMVLLLGACPE GCLVYEFM
Sbjct: 487 VYKCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFM 546

Query: 549 ANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRN 608
           ANGSLEDRL ++G++P LSWQ RF+IA EIGT LLFLHQTKPEPLVHRDLKPANILLDRN
Sbjct: 547 ANGSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRN 606

Query: 609 FVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF 668
           FVSK++DVGLARLVPPSVA+ VTQY MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF
Sbjct: 607 FVSKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF 666

Query: 669 LQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRP 726
           LQLIT +PPMGLTH+VERA+E     ++LDPAV+DWPVE+   FAK++LKCAE+RRKDRP
Sbjct: 667 LQLITGKPPMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLALKCAEIRRKDRP 726

BLAST of Carg21184 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 800.4 bits (2066), Expect = 1.2e-231
Identity = 434/756 (57.41%), Postives = 559/756 (73.94%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLP+ +  +KE    +G VAVAIDKDKGSQ ALKW ID+L  RGQT+ L+HV  RS S+
Sbjct: 1   MWLPKANGAKKE--TGSGSVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCRSHSS 60

Query: 61  SSL-----KMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIE 120
           S L     + K   E   ++LF+ F C+C+RK+I C+D++LED D  +A+TEY +++AIE
Sbjct: 61  SDLEEGTPQQKQQMEKIAKDLFVSFHCYCSRKEINCRDILLEDADKVRAITEYVSSSAIE 120

Query: 121 NIVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQS 180
           N+VVG++S++GF+R   TD+P  VSK APDFCNV+VISKGKI S+R+ASRPAP  N +Q 
Sbjct: 121 NLVVGSASRNGFMRRFKTDLPTTVSKSAPDFCNVYVISKGKIASVRNASRPAPYQNSMQQ 180

Query: 181 QLVQQSSTKSIRSDYQFPQTTSARASLDKRP----------------------------F 240
             +      +     ++    ++  S   RP                            F
Sbjct: 181 CEIDNHHPHTPDKAPKYHDHPNSAGSTPSRPRKSVEADRSPLVKRKPYGDFYDSDSDLSF 240

Query: 241 VLPRRSQD-EADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPM 300
           + P   +D +  F+SSGRPS++R   S + SLD    A   RMS+S++    QS  S  +
Sbjct: 241 ISPSSHRDSDISFISSGRPSVER---SSF-SLDFPESARTSRMSTSSE----QSIGSNRL 300

Query: 301 GRLSMDMNFPSEFSSISQDSDRL-SISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEAL 360
           G    D +F +E S+ S++S R  S SSQSL+DVEAEM+RL+LELKQTM+MYSTACKEAL
Sbjct: 301 GIKFSDPDFLNESSTFSEESGRTSSYSSQSLDDVEAEMKRLRLELKQTMDMYSTACKEAL 360

Query: 361 TAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQ 420
           +A+Q+A ELQ+ + EEE+R+EEA+ + EAA+++ E+E+AK+KAA+EAAEAA+R+AE+E++
Sbjct: 361 SARQQATELQKLRTEEERRLEEAKSSEEAAMSIVEKERAKAKAALEAAEAAKRLAEVESK 420

Query: 421 KRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVY 480
           +R+ AEMK  KE+       D+ S+  +RYRKYT++EIEEAT  FAES+K+GEGGYGPV+
Sbjct: 421 RRLTAEMKTMKES-------DSFSRGFVRYRKYTVDEIEEATSNFAESQKVGEGGYGPVF 480

Query: 481 KCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMAN 540
           +  LDHT VA+KVLRPDA QGRSQF++EVEVLSCIRHPNMVLLLGACPE+G LVYE+MA 
Sbjct: 481 RGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLSCIRHPNMVLLLGACPEFGILVYEYMAK 540

Query: 541 GSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFV 600
           GSLEDRL  RGNTPP++WQLRF+IA EI T LLFLHQTKPEP+VHRDLKP N+LLD N+V
Sbjct: 541 GSLEDRLFMRGNTPPITWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDYNYV 600

Query: 601 SKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQ 660
           SKISDVGLARLV P+VA+NVTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLGIM LQ
Sbjct: 601 SKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIMLLQ 660

Query: 661 LITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRPDL 720
           ++T++ PMGL ++VE+AIE     ++LDPAV DWP+EEALS AK+SL+CAELRRKDRPDL
Sbjct: 661 ILTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPIEEALSLAKLSLQCAELRRKDRPDL 720

Query: 721 GKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQ 722
           GKEILPELNRLR I E+ +           + ++SQ
Sbjct: 721 GKEILPELNRLREIGEESLESVFYAGNQGKSPNTSQ 738

BLAST of Carg21184 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 761.9 bits (1966), Expect = 4.6e-220
Identity = 425/759 (55.99%), Postives = 543/759 (71.54%), Query Frame = 0

Query: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN 60
           MWLP+   D  ++   NG VA+AIDKDK SQ A+KW +++L  RGQT+ L+HV  +S S+
Sbjct: 1   MWLPK--ADATKKGTRNGSVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHVLPKSQSS 60

Query: 61  ----SSLKMKSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIEN 120
                 +  K   E   ++LF+ F C+C+RK+I C DVVLED D  KA+ EY   +AIEN
Sbjct: 61  LDIEEGITHKQQMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIEN 120

Query: 121 IVVGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLQSQ 180
           +V+GA S++ F+R   TD+P +VSK APDFCNV+VISKGKI S+R+ASR AP  +P    
Sbjct: 121 LVLGAPSRNSFMRRFKTDLPTSVSKAAPDFCNVYVISKGKISSLRNASRLAP-YHPSVLS 180

Query: 181 LVQQSSTKSIRSDYQFPQTTS------------ARASLDKRP----------------FV 240
            V    T +I   ++   T +             R  L K P                F 
Sbjct: 181 EVDDHETIAIERKHKTANTPALPKGRRSIDSDVTRLGLPKPPHGHMKLMGDFSDSESEFS 240

Query: 241 LPRRSQDEA---DFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLP 300
               SQ E+    F+SSGRPS+DR   S + + D+   A   RMS+S++    QS  S  
Sbjct: 241 FISASQQESSDLSFISSGRPSVDR---SSF-TYDLPESARTSRMSTSSE----QSIGSHR 300

Query: 301 MGRLSMDMNFPSEFSSISQDSDR--LSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKE 360
           +G    D+++ +  SS+S +S R   S SSQSL DVEA+M+RL+LELKQTM+MYS+AC+E
Sbjct: 301 LGIKFTDLSYLNGSSSVSDESGRTSCSFSSQSLNDVEAQMKRLRLELKQTMDMYSSACRE 360

Query: 361 ALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELE 420
           ALTA+ +A ELQ+ + EEE+R+EE ++  E A+++ E E+AK+K A+EAAEAA R+AE+E
Sbjct: 361 ALTARNEATELQKLRTEEERRLEELKMTEETAMSIVENERAKAKTALEAAEAANRLAEVE 420

Query: 421 AQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFFAESRKIGEGGYGP 480
           A++R+ AEMK  KE++   R       S +RYRKY+++EIEE T  FAESRK+GEGGYGP
Sbjct: 421 AKRRVHAEMKVLKESDSFSR------HSIVRYRKYSVQEIEEGTANFAESRKVGEGGYGP 480

Query: 481 VYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFM 540
           V++  LDHT VA+KVLRPDA QGRSQF +EVEVLSCIRHPNMVLLLGACPEYG LVYE+M
Sbjct: 481 VFRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLSCIRHPNMVLLLGACPEYGILVYEYM 540

Query: 541 ANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRN 600
           A GSL+DRL +RGNTPP+SWQLRF+IA EI T LLFLHQTKPEP+VHRDLKP N+LLD N
Sbjct: 541 ARGSLDDRLFRRGNTPPISWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDHN 600

Query: 601 FVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMF 660
           +VSKISDVGLARLV P+VA+NVTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLGIM 
Sbjct: 601 YVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIML 660

Query: 661 LQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKMSLKCAELRRKDRP 720
           LQL+T++ PMGL ++VE+AIE     ++LDPAV DWP+EEALS AK+SL+CAELRRKDRP
Sbjct: 661 LQLLTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPLEEALSLAKLSLQCAELRRKDRP 720

BLAST of Carg21184 vs. TAIR 10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 753.8 bits (1945), Expect = 1.3e-217
Identity = 414/713 (58.06%), Postives = 521/713 (73.07%), Query Frame = 0

Query: 20  VAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSLSN--SSLKMKSDPEAGVRELF 79
           + +AID+DK SQ ALKWA+ +LL RGQT+ L+HVKL+  S+   S    S P     ELF
Sbjct: 12  ITLAIDRDKESQNALKWAVSNLLSRGQTLTLLHVKLKQPSSLPYSGSNFSKPGDDPSELF 71

Query: 80  LPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIVVGASSKSGFLRFKATDVPG 139
           LPFRC+C RKDI C+DVV+ED   AK + +Y    AIE +++G SSK   LRFKA DV  
Sbjct: 72  LPFRCYCARKDINCQDVVVEDVSAAKGIVDYVQQNAIETLILG-SSKMTLLRFKAADVSS 131

Query: 140 NVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVN--PLQSQLVQQSSTKSIRSDYQFPQT 199
            V K AP FC V+VISKGKI  +RSA+   P  N   ++     Q+S  ++    Q  Q 
Sbjct: 132 TVMKKAPSFCTVYVISKGKISFLRSATSSPPHSNMPSMRHHSHAQTSNMNVERRQQTMQR 191

Query: 200 TSARASLD-KRPF---VLPRRSQDEADFMSSGRPSIDRIFPSVYDSLDMGGRATPPRMSS 259
           T     ++ KR +     P  +  +  F+SSGRPS+D++FPS+YD +D+      PR+S 
Sbjct: 192 THDEIKIEIKRGYEGVYQPSITDSDISFVSSGRPSVDQMFPSLYDDVDV------PRLSV 251

Query: 260 STDLDLTQSFESLPMGRLSMDMNFP-SEFSSISQDSDRLSISSQSLEDVEAEMRRLKLEL 319
           +++    +   +    + S+D+  P +  SS S +S R S S Q  +++E EMRRLK+EL
Sbjct: 252 TSEYGENRLSFATTYSKQSIDLGSPYAPNSSTSFESGRQSFSLQGQDELETEMRRLKMEL 311

Query: 320 KQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAI 379
           K TMEMY++ACKEA++AK+ A EL +WK E+E ++EE RL+ EAA+A+AE+EK KS+AA+
Sbjct: 312 KHTMEMYNSACKEAISAKKAANELLKWKAEKEHKLEEVRLSKEAAMAMAEREKEKSRAAM 371

Query: 380 EAAEAARRIAELEAQKRMKAEMKAHKEAEEKKRAIDALSQSDIRYRKYTIEEIEEATEFF 439
           EAA AA+++++LEA+KR   E       +EKKRA+     S +RYRKYTIEEIEEATE F
Sbjct: 372 EAAVAAQKLSDLEAEKRKHIE-----TVDEKKRAV-----SSLRYRKYTIEEIEEATEDF 431

Query: 440 AESRKIGEGGYGPVYKCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLG 499
           + SRK+GEGGYGPVYK  LD+T VAIKVLRPDA QGRSQF++EVEVL+C+RHPNMVLLLG
Sbjct: 432 SPSRKVGEGGYGPVYKGTLDYTKVAIKVLRPDAAQGRSQFQREVEVLTCMRHPNMVLLLG 491

Query: 500 ACPEYGCLVYEFMANGSLEDRLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVH 559
           ACPEYGCLVYE+MANGSL+D L +RGN+P LSWQLRF+IA EI T L FLHQ KPEPLVH
Sbjct: 492 ACPEYGCLVYEYMANGSLDDCLFRRGNSPILSWQLRFRIASEIATGLHFLHQMKPEPLVH 551

Query: 560 RDLKPANILLDRNFVSKISDVGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGML 619
           RDLKP NILLD++FVSKISDVGLARLVPPSVAD  TQYRMTSTAGTF YIDPEYQQTGML
Sbjct: 552 RDLKPGNILLDQHFVSKISDVGLARLVPPSVADTATQYRMTSTAGTFFYIDPEYQQTGML 611

Query: 620 GVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENDMFANILDPAVTDWPVEEALSFAKM 679
           G KSDIYS GIM LQ++T++PPMGLTHHVE+AIE   FA +LDPAV DWP EEAL+ AK+
Sbjct: 612 GTKSDIYSFGIMLLQILTAKPPMGLTHHVEKAIEKGTFAEMLDPAVPDWPFEEALAAAKL 671

Query: 680 SLKCAELRRKDRPDLGKEILPELNRLRTIAEDYIHPTLMGDISSPTRSSSQVS 724
           +L+CA+LRRKDRPDLG  +LPEL +LR +AE+ I   +     SP RSS   +
Sbjct: 672 ALQCAKLRRKDRPDLGNIVLPELKKLRDLAEESIKFGVRQ--PSPIRSSGSAT 705

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037575.10.0e+00100.00U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022941502.10.0e+0095.62U-box domain-containing protein 52-like isoform X2 [Cucurbita moschata][more]
XP_022941501.10.0e+0095.50U-box domain-containing protein 52-like isoform X1 [Cucurbita moschata][more]
XP_023524077.10.0e+0094.83U-box domain-containing protein 52-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023524076.10.0e+0094.70U-box domain-containing protein 52-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SW115.5e-11738.50U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
Q9FKG61.0e-11536.52U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
Q9FKG53.3e-11439.02U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
Q8S8S71.1e-10637.91U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
Q9LU478.5e-10235.99Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1FML40.0e+0095.62U-box domain-containing protein 52-like isoform X2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1FLA10.0e+0095.50U-box domain-containing protein 52-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1IYC90.0e+0094.03U-box domain-containing protein 35-like isoform X2 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1J1M60.0e+0093.91U-box domain-containing protein 52-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1FSA40.0e+0095.22U-box domain-containing protein 52-like isoform X3 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT2G24370.19.5e-27464.88Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT4G31230.16.2e-25763.12Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G78940.21.2e-23157.41Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G16760.14.6e-22055.99Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT5G35380.11.3e-21758.06Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 356..402
NoneNo IPR availableCOILSCoilCoilcoord: 289..316
NoneNo IPR availableCOILSCoilCoilcoord: 322..342
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 407..502
e-value: 3.4E-25
score: 90.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 503..706
e-value: 4.2E-46
score: 158.8
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 102..174
e-value: 2.0
score: 5.1
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 12..200
coord: 218..724
NoneNo IPR availablePANTHERPTHR45647:SF101U-BOX DOMAIN-CONTAINING PROTEIN 34-LIKEcoord: 218..724
NoneNo IPR availablePANTHERPTHR45647:SF101U-BOX DOMAIN-CONTAINING PROTEIN 34-LIKEcoord: 12..200
NoneNo IPR availableCDDcd01989STK_Ncoord: 20..155
e-value: 3.0835E-59
score: 194.802
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 20..152
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 430..690
e-value: 2.5E-26
score: 103.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 431..683
e-value: 1.8E-39
score: 135.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 430..708
score: 35.000744
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 11..152
e-value: 4.6E-11
score: 45.0
IPR006016UspAPFAMPF00582Uspcoord: 21..144
e-value: 1.7E-10
score: 41.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 548..560
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 409..685

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21184-RACarg21184-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity