Homology
BLAST of Carg20770 vs. NCBI nr
Match:
KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 983.8 bits (2542), Expect = 5.4e-283
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTT 300
IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTT
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTT 300
Query: 301 TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 360
TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFL
Sbjct: 301 TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 360
Query: 361 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR 420
LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR
Sbjct: 361 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR 420
Query: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEP 480
FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEP
Sbjct: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEP 480
Query: 481 LKLLPKKRN 490
LKLLPKKRN
Sbjct: 481 LKLLPKKRN 489
BLAST of Carg20770 vs. NCBI nr
Match:
XP_022929108.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])
HSP 1 Score: 936.8 bits (2420), Expect = 7.6e-269
Identity = 469/490 (95.71%), Postives = 476/490 (97.14%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLPLI AILKHISSSSKQPP PPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF--LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGI+GHFKNVID ALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQIDSSWEILIKERRTIHSRGV +DFLDVLIQSG+T+EQINYLIVEVLSAGTD
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDL GRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKE 480
Query: 481 EPLKLLPKKR 489
EPLKLLPK+R
Sbjct: 481 EPLKLLPKRR 488
BLAST of Carg20770 vs. NCBI nr
Match:
XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])
HSP 1 Score: 935.6 bits (2417), Expect = 1.7e-268
Identity = 466/491 (94.91%), Postives = 479/491 (97.56%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLLPLI IL+HISSSSKQPPLPPGPKPWP+LGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF-LLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQ+DSSWEILIKERRTIHS+GVSAA+DFLDVLIQSG+T+EQI+YLIVEVLSAGTD
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKE 480
Query: 481 EPLKLLPKKRN 490
EPLKLL K RN
Sbjct: 481 EPLKLLSKNRN 490
BLAST of Carg20770 vs. NCBI nr
Match:
XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])
HSP 1 Score: 911.8 bits (2355), Expect = 2.6e-261
Identity = 457/491 (93.08%), Postives = 471/491 (95.93%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLLPLI IL+HI SSSKQPPLPPGP PWP+LGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF-LLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SH YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61 SHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMN+MVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLR FDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQ+DSSWEILIKERRTIHSR VSAA+DFLDVLI+SG+T+EQI+YLIVEVLSAGTD
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPK TMIFVNVWGIGRDPSIWEDP SFKPERFDG DADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEW LPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKE 480
Query: 481 EPLKLLPKKRN 490
EPLKLL KKRN
Sbjct: 481 EPLKLLSKKRN 490
BLAST of Carg20770 vs. NCBI nr
Match:
XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 911.0 bits (2353), Expect = 4.4e-261
Identity = 455/491 (92.67%), Postives = 471/491 (95.93%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLPLI +LKHISSSSKQPPLPPGP PWP+LGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF--LLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYI QM PDRALHDNCSL+FS
Sbjct: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKM +MVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQGSVVEIKEFVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSN IFSRDLV FVGDGFNGIKG FKN+IDL LIPNLADFYPLLR FDLQGLRKKAA
Sbjct: 181 FNTLSNLIFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQIDSS EILIKERRTIHSRGVSAA+DFLD+LI+SG+TNEQI+YLI+EVL AGTD
Sbjct: 241 IYKNQIDSSSEILIKERRTIHSRGVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAEL+KNRDIMNRVREETKKA+NGGAIDESKLSELHYLWKCVKET+RLHPPAP
Sbjct: 301 TTTVEWAMAELMKNRDIMNRVREETKKALNGGAIDESKLSELHYLWKCVKETVRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD DADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRR CPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDLNGRLGITLQKE
Sbjct: 421 YRFLPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKE 480
Query: 481 EPLKLLPKKRN 490
EPLKL+PKKRN
Sbjct: 481 EPLKLVPKKRN 489
BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match:
Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 427.6 bits (1098), Expect = 2.0e-118
Identity = 226/490 (46.12%), Postives = 309/490 (63.06%), Query Frame = 0
Query: 3 IASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSH 62
I SFSFLL L I S+ LPPGP+P P++GNLLQ+G H + +
Sbjct: 8 IVSFSFLLFLFF---------ILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQ 67
Query: 63 IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 122
YG L SLKLG+Q +VVASSPAAAA ILKT D++LS RY+FQ + H S+V+S E
Sbjct: 68 KYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENSIVWS-E 127
Query: 123 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 182
C D WK LR +CR +LFT + IESQ+ +R K EMV+FL+ K+G VV+I E VF T+ N
Sbjct: 128 CNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVN 187
Query: 183 TLSNFIFSRDLVGFVG--DGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 242
N IFS+D+ G +K H ++D+ N AD++P++ DL G R+ A
Sbjct: 188 IFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVA 247
Query: 243 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT- 302
QI W +++KERR ++G + DF+DVL+ +G +++IN L++E+ AGT+T
Sbjct: 248 EVLQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETS 307
Query: 303 -TTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 362
+T+EWA+ EL K +++++R E + + ES L L YL VKETLRLHPP P
Sbjct: 308 ASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTP 367
Query: 363 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 422
LLPR A E C +M Y++PK I VN W IGRDP W+DP +FKPERF D D KG+D
Sbjct: 368 LLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGND 427
Query: 423 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 482
+ +PFGGGRR+CPGLP+A+ LI+ATL+ NFEW LP + S L ++ + G+TLQK+
Sbjct: 428 FELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKD 484
Query: 483 EPLKLLPKKR 489
PL ++ K R
Sbjct: 488 PPLLIVLKAR 484
BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match:
O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 420.6 bits (1080), Expect = 2.4e-116
Identity = 215/482 (44.61%), Postives = 304/482 (63.07%), Query Frame = 0
Query: 12 LLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 71
L+ +I +IL + SS LPPGPKPWP++GNLLQ+G+ H + + YG + +LK
Sbjct: 8 LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 67
Query: 72 LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 131
+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ + H S+V+S +C + WKNLR
Sbjct: 68 MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLR 127
Query: 132 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 191
+CR +LFT + IESQA +R KK EMVE+L KQG V+I E +F T+ N N IFS+
Sbjct: 128 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 187
Query: 192 DL--VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 251
++ +G G + K + +++L N AD++P+L FDL G RK+ A I +
Sbjct: 188 NIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAI 247
Query: 252 WEILIKERRTIHS-RGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVEWAM 311
W +++ER+ G + DF+DV + SG + QIN L++E+ AGT+T +T+EWAM
Sbjct: 248 WGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 307
Query: 312 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 371
EL KN I ++R E + + ++ ES L YL VKETLRLHPP P LLPR A
Sbjct: 308 TELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRAL 367
Query: 372 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 431
E C ++ Y++PK I VN WGIGRDP W DP +F PERF D +G+D+ +PFG
Sbjct: 368 ETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGA 427
Query: 432 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPK 489
GRR+CPGLP+A + L++AT + N +WCLPN + L + + G+TLQKE PL ++PK
Sbjct: 428 GRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 487
BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match:
O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)
HSP 1 Score: 419.9 bits (1078), Expect = 4.1e-116
Identity = 214/482 (44.40%), Postives = 303/482 (62.86%), Query Frame = 0
Query: 12 LLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 71
L+ +I +IL + S LPPGPKPWP++GNLLQ+G+ H + + YG + +LK
Sbjct: 7 LIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 66
Query: 72 LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 131
+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ + H S+V+S +C + WKNLR
Sbjct: 67 MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLR 126
Query: 132 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 191
+CR +LFT + IESQA +R KK EMVE+L KQG V+I E +F T+ N N IFS+
Sbjct: 127 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 186
Query: 192 DL--VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 251
++ +G G + K + +++L N AD++P+L FDL G RK+ A I +
Sbjct: 187 NIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAI 246
Query: 252 WEILIKERRTIHS-RGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVEWAM 311
W +++ER+ G + DF+DV + SG + QIN L++E+ AGT+T +T+EWAM
Sbjct: 247 WGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 306
Query: 312 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 371
EL KN I ++R E + + ++ ES L YL VKETLRLHPP P LLPR A
Sbjct: 307 TELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRAL 366
Query: 372 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 431
E C ++ Y++PK I VN WGIGRDP W DP +F PERF D +G+D+ +PFG
Sbjct: 367 ETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGA 426
Query: 432 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPK 489
GRR+CPGLP+A + L++AT + N +WCLPN + L + + G+TLQKE PL ++PK
Sbjct: 427 GRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 486
BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match:
Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)
HSP 1 Score: 410.2 bits (1053), Expect = 3.2e-113
Identity = 212/482 (43.98%), Postives = 296/482 (61.41%), Query Frame = 0
Query: 12 LLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 71
L+ +I L I S LPPGPKPWP++GNLLQ+G+ H Q + YG L SLK
Sbjct: 2 LVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLK 61
Query: 72 LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 131
LG++ +VVAS+P AA+ ILKT DR+LS RY+FQ + H S+V+S EC + WK LR
Sbjct: 62 LGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLR 121
Query: 132 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 191
+CR +LFT + IESQA +R K EMVE+L+ G+ V+I E VF T+ N N IFS+
Sbjct: 122 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 181
Query: 192 DL--VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 251
++ +G G +K H +++L N AD++P L FDL G K A I S
Sbjct: 182 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 241
Query: 252 WEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVEWAMA 311
W ++KER+ S DF+++L+ SG ++QIN L++E+ AGT+T +T+EWA++
Sbjct: 242 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 301
Query: 312 ELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPE 371
EL KN + +R E + + ES + + YL VKETLRLHP P LLPR A E
Sbjct: 302 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 361
Query: 372 ACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGGG 431
C+++ Y++PK I VN WGIGRDP W DP F PERF D KG+D+ +PFG G
Sbjct: 362 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 421
Query: 432 RRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPKK 490
RR+CPG+P+A + LI+++L+ NF+W P + S L + + G+TLQKE PL ++PK
Sbjct: 422 RRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 481
BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match:
A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)
HSP 1 Score: 372.5 bits (955), Expect = 7.5e-102
Identity = 203/496 (40.93%), Postives = 309/496 (62.30%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNA----HIS 60
++IA FS + ++L + + +LK K LPPGP PWP++GNL + N HI+
Sbjct: 3 LQIALFSLIPVIL--VFILLLK-----PKYKNLPPGPHPWPLIGNLPILFTNTEVPLHIT 62
Query: 61 MTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCS 120
+ + +GP++ L LG Q V+AS+ AA ILKT DR+ SAR+I +M H S
Sbjct: 63 LANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYS 122
Query: 121 LVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFV 180
LV+S +C D WK LR I R ++F+ + +++Q+ +R +K+ E+++FL+SK+G VV+I +FV
Sbjct: 123 LVWS-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFV 182
Query: 181 FITVFNTLSNFIFSRDLVGFVGDGFN--GIKGHFKNVIDLALIPNLADFYPLLRCFDLQG 240
F T+ N L N +FS+D+ + GD + GI+ + ++ + PN+A+FYP L DLQG
Sbjct: 183 FGTLLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQG 242
Query: 241 LRKKAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLS 300
L+KK ++ WE +KER+ + + D LDVL+ + + + QIN L +E
Sbjct: 243 LKKKCDERFIRVMKMWEGTVKERKANRN---EESKDMLDVLLANDFNDAQINALFLETFG 302
Query: 301 AGTDTT--TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLR 360
G++T+ T+EW +AEL+K+ M +VR+E + + I ES L +L YL C+KE +R
Sbjct: 303 PGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMR 362
Query: 361 LHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDA 420
LHP APFLLPR A E CE+MGY++PK++ + VN + IGRDP W+DP +F PERF D
Sbjct: 363 LHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDV 422
Query: 421 DLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLG 480
D G Y+F+PFG GRR C G+P+A +PLI+ +L+HN+++ LP L +N L
Sbjct: 423 DFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLS 482
Query: 481 ITLQKEEPLKLLPKKR 489
+TL + L ++PK R
Sbjct: 483 LTLAIDPSLCVVPKAR 485
BLAST of Carg20770 vs. ExPASy TrEMBL
Match:
A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)
HSP 1 Score: 936.8 bits (2420), Expect = 3.7e-269
Identity = 469/490 (95.71%), Postives = 476/490 (97.14%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLPLI AILKHISSSSKQPP PPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF--LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGI+GHFKNVID ALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQIDSSWEILIKERRTIHSRGV +DFLDVLIQSG+T+EQINYLIVEVLSAGTD
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDL GRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKE 480
Query: 481 EPLKLLPKKR 489
EPLKLLPK+R
Sbjct: 481 EPLKLLPKRR 488
BLAST of Carg20770 vs. ExPASy TrEMBL
Match:
A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)
HSP 1 Score: 935.6 bits (2417), Expect = 8.2e-269
Identity = 466/491 (94.91%), Postives = 479/491 (97.56%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLLPLI IL+HISSSSKQPPLPPGPKPWP+LGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF-LLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQ+DSSWEILIKERRTIHS+GVSAA+DFLDVLIQSG+T+EQI+YLIVEVLSAGTD
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKE 480
Query: 481 EPLKLLPKKRN 490
EPLKLL K RN
Sbjct: 481 EPLKLLSKNRN 490
BLAST of Carg20770 vs. ExPASy TrEMBL
Match:
A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)
HSP 1 Score: 911.8 bits (2355), Expect = 1.3e-261
Identity = 457/491 (93.08%), Postives = 471/491 (95.93%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
MEIASFSF LLLLLPLI IL+HI SSSKQPPLPPGP PWP+LGNLLQIGQNAHISMTQF
Sbjct: 1 MEIASFSF-LLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60
Query: 61 SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
SH YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61 SHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120
Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMN+MVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 180
Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLR FDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240
Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
IYKNQ+DSSWEILIKERRTIHSR VSAA+DFLDVLI+SG+T+EQI+YLIVEVLSAGTD
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 300
Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
FLLPRYAPEACELMGYSVPK TMIFVNVWGIGRDPSIWEDP SFKPERFDG DADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHD 420
Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEW LPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKE 480
Query: 481 EPLKLLPKKRN 490
EPLKLL KKRN
Sbjct: 481 EPLKLLSKKRN 490
BLAST of Carg20770 vs. ExPASy TrEMBL
Match:
A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)
HSP 1 Score: 738.8 bits (1906), Expect = 1.5e-209
Identity = 370/498 (74.30%), Postives = 425/498 (85.34%), Query Frame = 0
Query: 1 MEIASFSFL---LLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISM 60
M I SFS L LLLLLP+ + ILKHI S S + PLPPGP PWP+LGNLLQIGQNAHIS+
Sbjct: 1 MLIPSFSILLPSLLLLLPIFI-ILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISI 60
Query: 61 TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 120
TQF++IYGPLISLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQM PDRALHD SL
Sbjct: 61 TQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSL 120
Query: 121 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 180
VFSPECGD+WKNLRS+C+ LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+FVF
Sbjct: 121 VFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVF 180
Query: 181 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK 240
+VFNTLSN IFS DL+ FVGDGF GIKG FK ++DL L PNLADFYPLLR FDLQGL+
Sbjct: 181 TSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKN 240
Query: 241 KAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGT 300
KA IYKN++DS W ILIKERR IH + SA+ DFLDVLIQSG+ ++QIN LI+E+LSAGT
Sbjct: 241 KADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGT 300
Query: 301 D--TTTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLH 360
D TTTVEWAM+E+LK++DI+N+VREE KK IDES +SEL YLW+CVKETLRLH
Sbjct: 301 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLH 360
Query: 361 PPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--- 420
PPAPFLLPR AP CE+MGYS+PK +M+FVNVWGIGRDPS+WED Q+F P+RFD GC
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 420
Query: 421 DADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGR 480
+ D KG+DYR+LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPNDE+ S LDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 480
Query: 481 LGITLQKEEPLKLLPKKR 489
LG+TLQK E LKL+PK+R
Sbjct: 481 LGVTLQKYETLKLIPKRR 497
BLAST of Carg20770 vs. ExPASy TrEMBL
Match:
A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)
HSP 1 Score: 738.0 bits (1904), Expect = 2.5e-209
Identity = 370/498 (74.30%), Postives = 425/498 (85.34%), Query Frame = 0
Query: 1 MEIASFSFL---LLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISM 60
M I SFS L LLLLLP+ + ILKHI S S + PLPPGP PWP+LGNLLQIGQNAHIS+
Sbjct: 1 MLIPSFSILLPSLLLLLPIFI-ILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISI 60
Query: 61 TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 120
TQF++IYGPLISLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQM PDRALHD SL
Sbjct: 61 TQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSL 120
Query: 121 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 180
VFSPECGD+WKNLRS+C+ LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+FVF
Sbjct: 121 VFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVF 180
Query: 181 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK 240
+VFNTLSN IFS DL+ FVGDGF GIKG FK ++DL L PNLADFYPLLR FDLQGL+
Sbjct: 181 TSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKN 240
Query: 241 KAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGT 300
KA IYKN++DS W ILIKERR IH + SA+ DFLDVLIQSG+ ++QIN LI+E+LSAGT
Sbjct: 241 KADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGT 300
Query: 301 D--TTTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLH 360
D TTTVEWAM+E+LK++DI+N+VREE KK IDES +SEL YLW+CVKETLRLH
Sbjct: 301 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLH 360
Query: 361 PPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--- 420
PPAPFLLPR AP CE+MGYS+PK +M+FVNVWGIGRDPS+WED Q+F P+RFD GC
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 420
Query: 421 DADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGR 480
+ D KG+DYR+LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPNDE+ S LDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 480
Query: 481 LGITLQKEEPLKLLPKKR 489
LG+TLQK E LKL+PK+R
Sbjct: 481 LGVTLQKYETLKLIPKRR 497
BLAST of Carg20770 vs. TAIR 10
Match:
AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )
HSP 1 Score: 353.6 bits (906), Expect = 2.5e-97
Identity = 200/483 (41.41%), Postives = 289/483 (59.83%), Query Frame = 0
Query: 26 SSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAA 85
SS K P PPGP P++GN+ +G+N H S S YGP++SLK G+ VV +SP A
Sbjct: 31 SSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEA 90
Query: 86 AAAILKTQDRLLSARYIFQMIPDRAL-HDNCSLVFSPECGDRWKNLRSICRAKLFTARAI 145
A +L+T D++LS+R I R++ HD S+V+ P RW+ LR + +LF+ + I
Sbjct: 91 AREVLRTYDQILSSRTPTNSI--RSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRI 150
Query: 146 ESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFN 205
E+ +LR K+ E+V F+ S++ V+I FIT N +SN +FS DL + +
Sbjct: 151 EATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNKSG 210
Query: 206 GIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK--KAA------IYKNQIDSSWEILI 265
+ V++ P+ A+F+P L DLQG RK KA +++ ID+ ++
Sbjct: 211 VFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFIDA--KLAE 270
Query: 266 KERRTIHSRGVSAATDFLDVLI------QSGYTNEQINYLIVEVLSAGTDT--TTVEWAM 325
K R +S+ V DF+DVL+ ++ I +L++++ AGTDT +TVEWAM
Sbjct: 271 KSLRDTNSKDV-RERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDTNSSTVEWAM 330
Query: 326 AELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 385
AELL+N + M + + E I G ++ES +S L YL VKET RLHP AP L+PR A
Sbjct: 331 AELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLHPAAPLLVPRKA 390
Query: 386 PEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFG 445
E++G+ VPK T +FVNVW IGRDP++WE+ FKPERF G D DL+G DY PFG
Sbjct: 391 ESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYELTPFG 450
Query: 446 GGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLP 489
GRR+CPGLP+A VPL+LA+L+++F+W LPN + LD++ G+TL K PL +P
Sbjct: 451 AGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 508
BLAST of Carg20770 vs. TAIR 10
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )
HSP 1 Score: 336.7 bits (862), Expect = 3.2e-92
Identity = 189/492 (38.41%), Postives = 284/492 (57.72%), Query Frame = 0
Query: 16 LIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLKLGAQ 75
LI + SS + LPPGP P++GN+ Q+G+N H S + IYGP++SLK G
Sbjct: 21 LISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCL 80
Query: 76 RLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICR 135
VV +SP AA +L+T D++LS R I H+ S+++ P RW+ LR +
Sbjct: 81 NSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSARWRMLRKLSV 140
Query: 136 AKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTLSNFIFSRDL 195
+F+ + E+ +LR KK+ E+V F+ S++ V+I + TV N +SN +FS DL
Sbjct: 141 TLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDL 200
Query: 196 VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKA--------AIYKNQ 255
+ N + ++ A P+ A+++P + DLQG RK +++
Sbjct: 201 GSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGF 260
Query: 256 IDSSWEILIKERRTIHSRGVSAATDFLDVLI------QSGYTNEQINYLIVEVLSAGTDT 315
+D+ + ++ +S+ VS DFLD L+ ++ N I +L++++ +AGTDT
Sbjct: 261 MDAK---IAEKSLGNYSKDVS-NRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDT 320
Query: 316 --TTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLHPP 375
+T+EWAMAELL+N M + + E + + + ES +S L YL VKET RLHP
Sbjct: 321 SSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPA 380
Query: 376 APFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKG 435
AP L+PR A E++G+ VPK T + VNVW IGRDPS+WE+P F+PERF G D D+KG
Sbjct: 381 APLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDVKG 440
Query: 436 HDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQ 489
DY PFGGGRR+CPGLP+A V L+LA+L+++F+W LPN + LD++ GITL
Sbjct: 441 RDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLH 500
BLAST of Carg20770 vs. TAIR 10
Match:
AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )
HSP 1 Score: 333.6 bits (854), Expect = 2.7e-91
Identity = 195/513 (38.01%), Postives = 298/513 (58.09%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQ-----PPLPPGPKPWPMLGNLLQIGQNAHI 60
M+I S LLLL ++ L ++ S + LPPGP P++GN+ +G++ H
Sbjct: 1 MDIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHR 60
Query: 61 SMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRAL-HDN 120
S + S YGP++SLKLG+ VV +SP AA +L+T D++LSAR + R++ H +
Sbjct: 61 SFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAV--RSINHQD 120
Query: 121 CSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEI 180
SLV+ P RW+ LR + +L + + IE+ +LR K+ E+V F+ S + V+I
Sbjct: 121 ASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDI 180
Query: 181 KEFVFITVFNTLSNFIFSRDLVGF-VGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFD 240
FIT N +SN +FS DL + NG++ +V+D A P+ A+++P LR D
Sbjct: 181 SRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLD 240
Query: 241 LQG--------LRKKAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLI-----QS 300
LQG + +++ ID+ I E+ + ++ + DF+D L+ +S
Sbjct: 241 LQGNVKTFKVCTERLVRVFRGFIDAK----IAEKSSQNNPKDVSKNDFVDNLLDYKGDES 300
Query: 301 GYTNEQINYLIVEVLSAGTDT--TTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDES 360
+ I +L++++ +AGTDT +T+EWAM ELLKN M + + E I G ++ES
Sbjct: 301 ELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEES 360
Query: 361 KLSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSI 420
+S+L YL VKET RLH P P L+PR A E++G+ V K T + VNVW IGRDPS+
Sbjct: 361 DISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSV 420
Query: 421 WEDPQSFKPERFDGCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWC 480
W++P F+PERF G D D++G DY PFG GRR+CPG+P+A V L+LA+L+++F+W
Sbjct: 421 WDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWK 480
Query: 481 LPNDENASTLDLNGRLGITLQKEEPLKLLPKKR 489
LP + LD++ G+TL K PL +P K+
Sbjct: 481 LPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKK 507
BLAST of Carg20770 vs. TAIR 10
Match:
AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 329.7 bits (844), Expect = 3.9e-90
Identity = 179/480 (37.29%), Postives = 278/480 (57.92%), Query Frame = 0
Query: 33 LPPGPKPWPMLGNLLQI-GQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILK 92
LPPGP PWP++GN+ Q+ G H S+T+ S +GP+++L++G+ VV SS A I K
Sbjct: 36 LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95
Query: 93 TQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLR 152
D L+ R I++ + D + + + G W+ LR +C + F R +++ + +R
Sbjct: 96 KHDAALAGRKIYEAMKGGKSSDGS--LITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155
Query: 153 RKKMNEMVEFLQ--SKQGS-VVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGHF 212
+ +++M+ F++ + G+ +++ + F+ FN + N +FSRDL+ + H
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFFYHT 215
Query: 213 KNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSSWEI---LIKERRTIHSRGV 272
V++ A PN+ADF+PLLR D QG+R+K + ++ ++EI I+ER + R
Sbjct: 216 GKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFH---VEKAFEIAGEFIRERTEVREREK 275
Query: 273 S--AATDFLDVLIQ-----------SGYTNEQINYLIVEVLSAGTDTT--TVEWAMAELL 332
S D+LDVL++ S +++ IN ++ E+ +AGTDTT T+EWA+AELL
Sbjct: 276 SDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELL 335
Query: 333 KNRDIMNRVREETKKAINGG--AIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPEA 392
N + +++ E + + E L L YL + ETLRLHPP PFL+P A
Sbjct: 336 HNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMST 395
Query: 393 CELMG-YSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERF--DGCDADLKGHDYRFLPFG 452
C + Y++PK T + VNVW IGRDP W DP FKPERF D D KG DY FLPFG
Sbjct: 396 CHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLPFG 455
Query: 453 GGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLP 486
GRR+CP LP+A+ +PL + +++ +F+W L N NA +D+ R+GITL+K PL+ +P
Sbjct: 456 SGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510
BLAST of Carg20770 vs. TAIR 10
Match:
AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )
HSP 1 Score: 325.9 bits (834), Expect = 5.7e-89
Identity = 199/512 (38.87%), Postives = 292/512 (57.03%), Query Frame = 0
Query: 1 MEIASFSFLLLLLLPLIVAILKHISSSSKQPPL-----PPGPKPWPMLGNLLQIGQNAHI 60
M+I S L L+ ++ +L ++ S++ P PPGP P++GN+ +G+N H
Sbjct: 1 MDIISGQPLFLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHH 60
Query: 61 SMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNC 120
S T S YGP++SLKLG VV +S A +LKT D++LS RYI + H
Sbjct: 61 SFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNN-HHEF 120
Query: 121 SLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS--KQGSVVEIK 180
S+ + R++ LR + +LF+ + I++ +LR KK+ E+V FL ++ V+I
Sbjct: 121 SVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDIS 180
Query: 181 EFVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQ 240
F+T N +SN +FS +L + + + + P+LA+F+P +R DLQ
Sbjct: 181 HVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQ 240
Query: 241 GLRKK--------AAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLI--QSGYTNE 300
G KK +++ D+ I+ K R++ + VS + DFLDVLI Q G E
Sbjct: 241 GNSKKMRESSGRLLQVFREFYDA--RIVEKSSRSV-EKDVS-SKDFLDVLIDLQQGDETE 300
Query: 301 ----QINYLIVEVLSAGTDT--TTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESK 360
+I +L++++ AGTDT +TVEWAMAELL N M +V++E I G ES
Sbjct: 301 INIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESD 360
Query: 361 LSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIW 420
+S+L YL VKET RLHP APFLL R A E++G++V K + + VNVW IGRDP +W
Sbjct: 361 ISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVW 420
Query: 421 EDPQSFKPERFDGCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCL 480
E+P F+PERF G + D+KG DY PFG GRR+CPGLP+A V L+LA+L++ FEW L
Sbjct: 421 ENPTHFEPERFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKL 480
Query: 481 PNDENASTLDLNGRLGITLQKEEPLKLLPKKR 489
PN + LD+ G+T+ K PL +P K+
Sbjct: 481 PNGVGSEDLDMEETFGLTVHKTNPLLAVPLKK 507
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| KAG7017133.1 | 5.4e-283 | 100.00 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma... | [more] |
| XP_022929108.1 | 7.6e-269 | 95.71 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata] | [more] |
| XP_022928791.1 | 1.7e-268 | 94.91 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata] | [more] |
| XP_022970204.1 | 2.6e-261 | 93.08 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima] | [more] |
| XP_023551257.1 | 4.4e-261 | 92.67 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9FXW4 | 2.0e-118 | 46.12 | Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... | [more] |
| O64900 | 2.4e-116 | 44.61 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... | [more] |
| O64899 | 4.1e-116 | 44.40 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... | [more] |
| Q9SP06 | 3.2e-113 | 43.98 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... | [more] |
| A8CDR5 | 7.5e-102 | 40.93 | Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1ER52 | 3.7e-269 | 95.71 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
| A0A6J1ELA6 | 8.2e-269 | 94.91 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
| A0A6J1I4V7 | 1.3e-261 | 93.08 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... | [more] |
| A0A5A7UIU3 | 1.5e-209 | 74.30 | Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... | [more] |
| A0A1S3CT14 | 2.5e-209 | 74.30 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT2G45570.1 | 2.5e-97 | 41.41 | cytochrome P450, family 76, subfamily C, polypeptide 2 | [more] |
| AT2G45550.1 | 3.2e-92 | 38.41 | cytochrome P450, family 76, subfamily C, polypeptide 4 | [more] |
| AT2G45560.1 | 2.7e-91 | 38.01 | cytochrome P450, family 76, subfamily C, polypeptide 1 | [more] |
| AT3G52970.1 | 3.9e-90 | 37.29 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
| AT1G33720.1 | 5.7e-89 | 38.87 | cytochrome P450, family 76, subfamily C, polypeptide 6 | [more] |
