Carg20770 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20770
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cytochrome p450 76c2
Location	Carg_Chr15: 9938874 .. 9940950 (+)
RNA-Seq Expression	Carg20770
Synteny	Carg20770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATTGCTTCATTTTCCTTTCTTCTTCTTCTTCTACTACCTCTAATCGTCGCCATTCTTAAGCACATCAGTTCATCTTCAAAACAGCCGCCACTTCCTCCGGGTCCAAAGCCATGGCCGATGCTTGGAAATCTCCTTCAGATCGGTCAAAACGCCCACATTTCAATGACCCAATTCTCTCATATCTACGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGCTTGGTGGTCGCTTCTTCACCGGCCGCCGCCGCCGCCATTCTCAAGACTCAAGATCGCCTTCTATCTGCTCGATACATTTTCCAAATGATACCTGATCGAGCTCTGCACGATAACTGCTCGCTTGTTTTCTCACCGGAATGCGGCGATCGTTGGAAGAATCTGAGATCCATTTGCAGAGCCAAATTGTTTACAGCGAGGGCGATCGAATCCCAAGCCAGTTTGAGAAGGAAGAAGATGAATGAAATGGTGGAGTTCTTGCAGTCAAAACAGGGATCTGTGGTGGAGATTAAAGAATTTGTGTTTATTACTGTTTTCAACACTCTTTCTAACTTCATCTTCTCTAGGGATTTGGTTGGTTTTGTGGGTGATGGGTTCAATGGGATTAAAGGGCATTTCAAGAACGTGATCGATTTGGCTTTGATCCCAAATTTGGCTGACTTTTACCCTTTGCTCAGATGCTTCGATCTTCAAGGACTCCGAAAGAAAGCTGCTATCTATAAGAACCAAATCGATTCTTCCTGGGAAATTCTTATTAAAGAACGAAGAACAATTCATAGTCGAGGCGTTTCAGCTGCGACCGATTTCTTGGATGTTTTGATCCAAAGTGGATATACCAATGAACAAATTAACTACTTGATTGTTGTAAGTGAAAAAAAAAATCATTGAGGGAGTCTCACACTAATTGGTACTATGCATGTCTAGTGGTAGGAGGTATTTCGGGAAGCTCAAAGAAAAGTCACAGAGTCTATGCTCAAAGTGGACAATATATCATATCATTGTAGAGAGTCGTGATTTCTAATATGGTATCAGAATCATGCCCTTAACTTAGTCATGTCAATAGAATTCTCAAATGTCGAACAAAAAAGTTGTGAGGCTTGAAAGTATAGTCAAAAGTGACTTAAGTGTCGAACACAGGGTATAGTTTGTTCGATGACTCTAGAGAAGGAGTCGAGCCTCGATTAAGGAGAGGCTGTTCGAAGACTTCATAAGGTTCAGGAGAGACTCTACCGTGTACTTTGTTCTAGAGGAGAATTGTTGGGAGGGAGGATTAAGGAGGGCTTCCGTATAAGTAAGGAATATATCTCCACTCCTGTGAAGTTTTTTTGGGAAAGCCGAAAACAAAGCCACTAAAGCTTATGCTCAAAGTGGATCATATCATACTATCGAACCAGAGTCGTGATTCCTAACAAAAACTCAAATTTTGTTAGATGGGTAGTTTTCTAATCAAAGTTTTGATGGTTTATTGCAGGAAGTGTTGTCCGCCGGCACGGATACAACGACGGTGGAGTGGGCGATGGCGGAGCTGTTGAAGAACAGAGACATAATGAACAGAGTGCGAGAGGAAACAAAGAAGGCAATAAACGGAGGCGCCATTGACGAATCAAAGCTCTCTGAGCTTCATTATCTATGGAAATGCGTGAAAGAAACTCTACGGCTGCATCCGCCTGCACCATTTCTTCTTCCACGGTATGCTCCAGAAGCTTGTGAGCTTATGGGGTACTCTGTTCCTAAACACACCATGATTTTTGTGAATGTTTGGGGGATTGGGCGAGACCCTTCAATCTGGGAAGATCCTCAATCGTTCAAACCAGAAAGATTTGATGGCTGTGATGCAGATTTGAAGGGTCATGATTATCGATTCCTGCCATTTGGAGGAGGCAGAAGGCTTTGCCCTGGGCTTCCCATGGCGGCGGTGCAAGTGCCTTTAATCTTAGCCACCTTGATTCATAACTTTGAATGGTGCCTTCCAAATGATGAGAATGCGTCAACGCTGGATTTGAATGGAAGGCTTGGTATAACGTTGCAGAAGGAAGAACCTCTTAAGCTCCTCCCCAAAAAGAGGAACTGA

mRNA sequence

ATGGAGATTGCTTCATTTTCCTTTCTTCTTCTTCTTCTACTACCTCTAATCGTCGCCATTCTTAAGCACATCAGTTCATCTTCAAAACAGCCGCCACTTCCTCCGGGTCCAAAGCCATGGCCGATGCTTGGAAATCTCCTTCAGATCGGTCAAAACGCCCACATTTCAATGACCCAATTCTCTCATATCTACGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGCTTGGTGGTCGCTTCTTCACCGGCCGCCGCCGCCGCCATTCTCAAGACTCAAGATCGCCTTCTATCTGCTCGATACATTTTCCAAATGATACCTGATCGAGCTCTGCACGATAACTGCTCGCTTGTTTTCTCACCGGAATGCGGCGATCGTTGGAAGAATCTGAGATCCATTTGCAGAGCCAAATTGTTTACAGCGAGGGCGATCGAATCCCAAGCCAGTTTGAGAAGGAAGAAGATGAATGAAATGGTGGAGTTCTTGCAGTCAAAACAGGGATCTGTGGTGGAGATTAAAGAATTTGTGTTTATTACTGTTTTCAACACTCTTTCTAACTTCATCTTCTCTAGGGATTTGGTTGGTTTTGTGGGTGATGGGTTCAATGGGATTAAAGGGCATTTCAAGAACGTGATCGATTTGGCTTTGATCCCAAATTTGGCTGACTTTTACCCTTTGCTCAGATGCTTCGATCTTCAAGGACTCCGAAAGAAAGCTGCTATCTATAAGAACCAAATCGATTCTTCCTGGGAAATTCTTATTAAAGAACGAAGAACAATTCATAGTCGAGGCGTTTCAGCTGCGACCGATTTCTTGGATGTTTTGATCCAAAGTGGATATACCAATGAACAAATTAACTACTTGATTGTTGAAGTGTTGTCCGCCGGCACGGATACAACGACGGTGGAGTGGGCGATGGCGGAGCTGTTGAAGAACAGAGACATAATGAACAGAGTGCGAGAGGAAACAAAGAAGGCAATAAACGGAGGCGCCATTGACGAATCAAAGCTCTCTGAGCTTCATTATCTATGGAAATGCGTGAAAGAAACTCTACGGCTGCATCCGCCTGCACCATTTCTTCTTCCACGGTATGCTCCAGAAGCTTGTGAGCTTATGGGGTACTCTGTTCCTAAACACACCATGATTTTTGTGAATGTTTGGGGGATTGGGCGAGACCCTTCAATCTGGGAAGATCCTCAATCGTTCAAACCAGAAAGATTTGATGGCTGTGATGCAGATTTGAAGGGTCATGATTATCGATTCCTGCCATTTGGAGGAGGCAGAAGGCTTTGCCCTGGGCTTCCCATGGCGGCGGTGCAAGTGCCTTTAATCTTAGCCACCTTGATTCATAACTTTGAATGGTGCCTTCCAAATGATGAGAATGCGTCAACGCTGGATTTGAATGGAAGGCTTGGTATAACGTTGCAGAAGGAAGAACCTCTTAAGCTCCTCCCCAAAAAGAGGAACTGA

Coding sequence (CDS)

ATGGAGATTGCTTCATTTTCCTTTCTTCTTCTTCTTCTACTACCTCTAATCGTCGCCATTCTTAAGCACATCAGTTCATCTTCAAAACAGCCGCCACTTCCTCCGGGTCCAAAGCCATGGCCGATGCTTGGAAATCTCCTTCAGATCGGTCAAAACGCCCACATTTCAATGACCCAATTCTCTCATATCTACGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGCTTGGTGGTCGCTTCTTCACCGGCCGCCGCCGCCGCCATTCTCAAGACTCAAGATCGCCTTCTATCTGCTCGATACATTTTCCAAATGATACCTGATCGAGCTCTGCACGATAACTGCTCGCTTGTTTTCTCACCGGAATGCGGCGATCGTTGGAAGAATCTGAGATCCATTTGCAGAGCCAAATTGTTTACAGCGAGGGCGATCGAATCCCAAGCCAGTTTGAGAAGGAAGAAGATGAATGAAATGGTGGAGTTCTTGCAGTCAAAACAGGGATCTGTGGTGGAGATTAAAGAATTTGTGTTTATTACTGTTTTCAACACTCTTTCTAACTTCATCTTCTCTAGGGATTTGGTTGGTTTTGTGGGTGATGGGTTCAATGGGATTAAAGGGCATTTCAAGAACGTGATCGATTTGGCTTTGATCCCAAATTTGGCTGACTTTTACCCTTTGCTCAGATGCTTCGATCTTCAAGGACTCCGAAAGAAAGCTGCTATCTATAAGAACCAAATCGATTCTTCCTGGGAAATTCTTATTAAAGAACGAAGAACAATTCATAGTCGAGGCGTTTCAGCTGCGACCGATTTCTTGGATGTTTTGATCCAAAGTGGATATACCAATGAACAAATTAACTACTTGATTGTTGAAGTGTTGTCCGCCGGCACGGATACAACGACGGTGGAGTGGGCGATGGCGGAGCTGTTGAAGAACAGAGACATAATGAACAGAGTGCGAGAGGAAACAAAGAAGGCAATAAACGGAGGCGCCATTGACGAATCAAAGCTCTCTGAGCTTCATTATCTATGGAAATGCGTGAAAGAAACTCTACGGCTGCATCCGCCTGCACCATTTCTTCTTCCACGGTATGCTCCAGAAGCTTGTGAGCTTATGGGGTACTCTGTTCCTAAACACACCATGATTTTTGTGAATGTTTGGGGGATTGGGCGAGACCCTTCAATCTGGGAAGATCCTCAATCGTTCAAACCAGAAAGATTTGATGGCTGTGATGCAGATTTGAAGGGTCATGATTATCGATTCCTGCCATTTGGAGGAGGCAGAAGGCTTTGCCCTGGGCTTCCCATGGCGGCGGTGCAAGTGCCTTTAATCTTAGCCACCTTGATTCATAACTTTGAATGGTGCCTTCCAAATGATGAGAATGCGTCAACGCTGGATTTGAATGGAAGGCTTGGTATAACGTTGCAGAAGGAAGAACCTCTTAAGCTCCTCCCCAAAAAGAGGAACTGA

Protein sequence

MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPKKRN

Homology

[Close]

BLAST of Carg20770 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 983.8 bits (2542), Expect = 5.4e-283
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTT 300
           IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTT
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDTT 300

Query: 301 TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 360
           TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFL
Sbjct: 301 TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 360

Query: 361 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR 420
           LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR
Sbjct: 361 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR 420

Query: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEP 480
           FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEP
Sbjct: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEP 480

Query: 481 LKLLPKKRN 490
           LKLLPKKRN
Sbjct: 481 LKLLPKKRN 489

[Close]

BLAST of Carg20770 vs. NCBI nr
Match: XP_022929108.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 936.8 bits (2420), Expect = 7.6e-269
Identity = 469/490 (95.71%), Postives = 476/490 (97.14%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF  LLLLPLI AILKHISSSSKQPP PPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF--LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGI+GHFKNVID ALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQIDSSWEILIKERRTIHSRGV   +DFLDVLIQSG+T+EQINYLIVEVLSAGTD  
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDL GRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKE 480

Query: 481 EPLKLLPKKR 489
           EPLKLLPK+R
Sbjct: 481 EPLKLLPKRR 488

[Close]

BLAST of Carg20770 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 935.6 bits (2417), Expect = 1.7e-268
Identity = 466/491 (94.91%), Postives = 479/491 (97.56%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF LLLLLPLI  IL+HISSSSKQPPLPPGPKPWP+LGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF-LLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQ+DSSWEILIKERRTIHS+GVSAA+DFLDVLIQSG+T+EQI+YLIVEVLSAGTD  
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKE 480

Query: 481 EPLKLLPKKRN 490
           EPLKLL K RN
Sbjct: 481 EPLKLLSKNRN 490

[Close]

BLAST of Carg20770 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 911.8 bits (2355), Expect = 2.6e-261
Identity = 457/491 (93.08%), Postives = 471/491 (95.93%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF LLLLLPLI  IL+HI SSSKQPPLPPGP PWP+LGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF-LLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SH YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61  SHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMN+MVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLR FDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQ+DSSWEILIKERRTIHSR VSAA+DFLDVLI+SG+T+EQI+YLIVEVLSAGTD  
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPK TMIFVNVWGIGRDPSIWEDP SFKPERFDG DADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEW LPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKE 480

Query: 481 EPLKLLPKKRN 490
           EPLKLL KKRN
Sbjct: 481 EPLKLLSKKRN 490

[Close]

BLAST of Carg20770 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911.0 bits (2353), Expect = 4.4e-261
Identity = 455/491 (92.67%), Postives = 471/491 (95.93%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF  LLLLPLI  +LKHISSSSKQPPLPPGP PWP+LGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF--LLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYI QM PDRALHDNCSL+FS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKM +MVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQGSVVEIKEFVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSN IFSRDLV FVGDGFNGIKG FKN+IDL LIPNLADFYPLLR FDLQGLRKKAA
Sbjct: 181 FNTLSNLIFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQIDSS EILIKERRTIHSRGVSAA+DFLD+LI+SG+TNEQI+YLI+EVL AGTD  
Sbjct: 241 IYKNQIDSSSEILIKERRTIHSRGVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAEL+KNRDIMNRVREETKKA+NGGAIDESKLSELHYLWKCVKET+RLHPPAP
Sbjct: 301 TTTVEWAMAELMKNRDIMNRVREETKKALNGGAIDESKLSELHYLWKCVKETVRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD  DADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRR CPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDLNGRLGITLQKE
Sbjct: 421 YRFLPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKE 480

Query: 481 EPLKLLPKKRN 490
           EPLKL+PKKRN
Sbjct: 481 EPLKLVPKKRN 489

[Close]

BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 2.0e-118
Identity = 226/490 (46.12%), Postives = 309/490 (63.06%), Query Frame = 0


Query: 3   IASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSH 62
           I SFSFLL L           I   S+   LPPGP+P P++GNLLQ+G   H    + + 
Sbjct: 8   IVSFSFLLFLFF---------ILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQ 67

Query: 63  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 122
            YG L SLKLG+Q +VVASSPAAAA ILKT D++LS RY+FQ    +  H   S+V+S E
Sbjct: 68  KYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENSIVWS-E 127

Query: 123 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 182
           C D WK LR +CR +LFT + IESQ+ +R  K  EMV+FL+ K+G VV+I E VF T+ N
Sbjct: 128 CNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVN 187

Query: 183 TLSNFIFSRDLVGFVG--DGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 242
              N IFS+D+        G   +K H   ++D+    N AD++P++   DL G R+  A
Sbjct: 188 IFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVA 247

Query: 243 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT- 302
               QI   W +++KERR   ++G  +  DF+DVL+ +G  +++IN L++E+  AGT+T 
Sbjct: 248 EVLQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETS 307

Query: 303 -TTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 362
            +T+EWA+ EL K   +++++R E    +    + ES L  L YL   VKETLRLHPP P
Sbjct: 308 ASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTP 367

Query: 363 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 422
            LLPR A E C +M Y++PK   I VN W IGRDP  W+DP +FKPERF   D D KG+D
Sbjct: 368 LLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGND 427

Query: 423 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 482
           +  +PFGGGRR+CPGLP+A+    LI+ATL+ NFEW LP   + S L ++ + G+TLQK+
Sbjct: 428 FELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKD 484

Query: 483 EPLKLLPKKR 489
            PL ++ K R
Sbjct: 488 PPLLIVLKAR 484

[Close]

BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.4e-116
Identity = 215/482 (44.61%), Postives = 304/482 (63.07%), Query Frame = 0


Query: 12  LLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 71
           L+  +I +IL  +  SS    LPPGPKPWP++GNLLQ+G+  H    + +  YG + +LK
Sbjct: 8   LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 67

Query: 72  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 131
           +G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C + WKNLR
Sbjct: 68  MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLR 127

Query: 132 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 191
            +CR +LFT + IESQA +R KK  EMVE+L  KQG  V+I E +F T+ N   N IFS+
Sbjct: 128 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 187

Query: 192 DL--VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 251
           ++  +G    G +  K +   +++L    N AD++P+L  FDL G RK+ A     I + 
Sbjct: 188 NIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAI 247

Query: 252 WEILIKERRTIHS-RGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVEWAM 311
           W  +++ER+      G  +  DF+DV + SG  + QIN L++E+  AGT+T  +T+EWAM
Sbjct: 248 WGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 307

Query: 312 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 371
            EL KN  I  ++R E +  +   ++ ES    L YL   VKETLRLHPP P LLPR A 
Sbjct: 308 TELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRAL 367

Query: 372 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 431
           E C ++ Y++PK   I VN WGIGRDP  W DP +F PERF     D +G+D+  +PFG 
Sbjct: 368 ETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGA 427

Query: 432 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPK 489
           GRR+CPGLP+A   + L++AT + N +WCLPN  +   L +  + G+TLQKE PL ++PK
Sbjct: 428 GRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 487

[Close]

BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.1e-116
Identity = 214/482 (44.40%), Postives = 303/482 (62.86%), Query Frame = 0


Query: 12  LLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 71
           L+  +I +IL  +   S    LPPGPKPWP++GNLLQ+G+  H    + +  YG + +LK
Sbjct: 7   LIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 66

Query: 72  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 131
           +G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C + WKNLR
Sbjct: 67  MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLR 126

Query: 132 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 191
            +CR +LFT + IESQA +R KK  EMVE+L  KQG  V+I E +F T+ N   N IFS+
Sbjct: 127 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 186

Query: 192 DL--VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 251
           ++  +G    G +  K +   +++L    N AD++P+L  FDL G RK+ A     I + 
Sbjct: 187 NIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAI 246

Query: 252 WEILIKERRTIHS-RGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVEWAM 311
           W  +++ER+      G  +  DF+DV + SG  + QIN L++E+  AGT+T  +T+EWAM
Sbjct: 247 WGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 306

Query: 312 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 371
            EL KN  I  ++R E +  +   ++ ES    L YL   VKETLRLHPP P LLPR A 
Sbjct: 307 TELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRAL 366

Query: 372 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 431
           E C ++ Y++PK   I VN WGIGRDP  W DP +F PERF     D +G+D+  +PFG 
Sbjct: 367 ETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGA 426

Query: 432 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPK 489
           GRR+CPGLP+A   + L++AT + N +WCLPN  +   L +  + G+TLQKE PL ++PK
Sbjct: 427 GRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 486

[Close]

BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.2e-113
Identity = 212/482 (43.98%), Postives = 296/482 (61.41%), Query Frame = 0


Query: 12  LLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 71
           L+  +I   L  I   S    LPPGPKPWP++GNLLQ+G+  H    Q +  YG L SLK
Sbjct: 2   LVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLK 61

Query: 72  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 131
           LG++ +VVAS+P AA+ ILKT DR+LS RY+FQ    +  H   S+V+S EC + WK LR
Sbjct: 62  LGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLR 121

Query: 132 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 191
            +CR +LFT + IESQA +R  K  EMVE+L+   G+ V+I E VF T+ N   N IFS+
Sbjct: 122 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 181

Query: 192 DL--VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 251
           ++  +G    G   +K H   +++L    N AD++P L  FDL G  K  A     I S 
Sbjct: 182 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 241

Query: 252 WEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TTVEWAMA 311
           W  ++KER+       S   DF+++L+ SG  ++QIN L++E+  AGT+T  +T+EWA++
Sbjct: 242 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 301

Query: 312 ELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPE 371
           EL KN  +   +R E    +    + ES +  + YL   VKETLRLHP  P LLPR A E
Sbjct: 302 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 361

Query: 372 ACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGGG 431
            C+++ Y++PK   I VN WGIGRDP  W DP  F PERF     D KG+D+  +PFG G
Sbjct: 362 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 421

Query: 432 RRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLPKK 490
           RR+CPG+P+A   + LI+++L+ NF+W  P   + S L +  + G+TLQKE PL ++PK 
Sbjct: 422 RRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 481

[Close]

BLAST of Carg20770 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 7.5e-102
Identity = 203/496 (40.93%), Postives = 309/496 (62.30%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNA----HIS 60
           ++IA FS + ++L  + + +LK      K   LPPGP PWP++GNL  +  N     HI+
Sbjct: 3   LQIALFSLIPVIL--VFILLLK-----PKYKNLPPGPHPWPLIGNLPILFTNTEVPLHIT 62

Query: 61  MTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCS 120
           +   +  +GP++ L LG Q  V+AS+  AA  ILKT DR+ SAR+I +M      H   S
Sbjct: 63  LANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYS 122

Query: 121 LVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFV 180
           LV+S +C D WK LR I R ++F+ + +++Q+ +R +K+ E+++FL+SK+G VV+I +FV
Sbjct: 123 LVWS-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFV 182

Query: 181 FITVFNTLSNFIFSRDLVGFVGDGFN--GIKGHFKNVIDLALIPNLADFYPLLRCFDLQG 240
           F T+ N L N +FS+D+  + GD  +  GI+   + ++ +   PN+A+FYP L   DLQG
Sbjct: 183 FGTLLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQG 242

Query: 241 LRKKAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLS 300
           L+KK      ++   WE  +KER+   +     + D LDVL+ + + + QIN L +E   
Sbjct: 243 LKKKCDERFIRVMKMWEGTVKERKANRN---EESKDMLDVLLANDFNDAQINALFLETFG 302

Query: 301 AGTDTT--TVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLR 360
            G++T+  T+EW +AEL+K+   M +VR+E  + +    I ES L +L YL  C+KE +R
Sbjct: 303 PGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMR 362

Query: 361 LHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDA 420
           LHP APFLLPR A E CE+MGY++PK++ + VN + IGRDP  W+DP +F PERF   D 
Sbjct: 363 LHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDV 422

Query: 421 DLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLG 480
           D  G  Y+F+PFG GRR C G+P+A   +PLI+ +L+HN+++ LP       L +N  L 
Sbjct: 423 DFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLS 482

Query: 481 ITLQKEEPLKLLPKKR 489
           +TL  +  L ++PK R
Sbjct: 483 LTLAIDPSLCVVPKAR 485

[Close]

BLAST of Carg20770 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 3.7e-269
Identity = 469/490 (95.71%), Postives = 476/490 (97.14%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF  LLLLPLI AILKHISSSSKQPP PPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF--LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGI+GHFKNVID ALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQIDSSWEILIKERRTIHSRGV   +DFLDVLIQSG+T+EQINYLIVEVLSAGTD  
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDL GRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKE 480

Query: 481 EPLKLLPKKR 489
           EPLKLLPK+R
Sbjct: 481 EPLKLLPKRR 488

[Close]

BLAST of Carg20770 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 8.2e-269
Identity = 466/491 (94.91%), Postives = 479/491 (97.56%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF LLLLLPLI  IL+HISSSSKQPPLPPGPKPWP+LGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF-LLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQ+DSSWEILIKERRTIHS+GVSAA+DFLDVLIQSG+T+EQI+YLIVEVLSAGTD  
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKE 480

Query: 481 EPLKLLPKKRN 490
           EPLKLL K RN
Sbjct: 481 EPLKLLSKNRN 490

[Close]

BLAST of Carg20770 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.3e-261
Identity = 457/491 (93.08%), Postives = 471/491 (95.93%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF LLLLLPLI  IL+HI SSSKQPPLPPGP PWP+LGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSF-LLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SH YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FS
Sbjct: 61  SHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMN+MVEFLQSKQGSVVEIKE VFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGIKG FKN+IDL LIPNLADFYPLLR FDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300
           IYKNQ+DSSWEILIKERRTIHSR VSAA+DFLDVLI+SG+T+EQI+YLIVEVLSAGTD  
Sbjct: 241 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420
           FLLPRYAPEACELMGYSVPK TMIFVNVWGIGRDPSIWEDP SFKPERFDG DADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480
           YRFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEW LPNDE+ASTLDLNGRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKE 480

Query: 481 EPLKLLPKKRN 490
           EPLKLL KKRN
Sbjct: 481 EPLKLLSKKRN 490

[Close]

BLAST of Carg20770 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.5e-209
Identity = 370/498 (74.30%), Postives = 425/498 (85.34%), Query Frame = 0


Query: 1   MEIASFSFL---LLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISM 60
           M I SFS L   LLLLLP+ + ILKHI S S + PLPPGP PWP+LGNLLQIGQNAHIS+
Sbjct: 1   MLIPSFSILLPSLLLLLPIFI-ILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISI 60

Query: 61  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 120
           TQF++IYGPLISLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQM PDRALHD  SL
Sbjct: 61  TQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSL 120

Query: 121 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 180
           VFSPECGD+WKNLRS+C+  LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+FVF
Sbjct: 121 VFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVF 180

Query: 181 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK 240
            +VFNTLSN IFS DL+ FVGDGF GIKG FK ++DL L PNLADFYPLLR FDLQGL+ 
Sbjct: 181 TSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKN 240

Query: 241 KAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGT 300
           KA IYKN++DS W ILIKERR IH +  SA+ DFLDVLIQSG+ ++QIN LI+E+LSAGT
Sbjct: 241 KADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGT 300

Query: 301 D--TTTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLH 360
           D  TTTVEWAM+E+LK++DI+N+VREE KK       IDES +SEL YLW+CVKETLRLH
Sbjct: 301 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLH 360

Query: 361 PPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--- 420
           PPAPFLLPR AP  CE+MGYS+PK +M+FVNVWGIGRDPS+WED Q+F P+RFD GC   
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 420

Query: 421 DADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGR 480
           + D KG+DYR+LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPNDE+ S LDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 480

Query: 481 LGITLQKEEPLKLLPKKR 489
           LG+TLQK E LKL+PK+R
Sbjct: 481 LGVTLQKYETLKLIPKRR 497

[Close]

BLAST of Carg20770 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.5e-209
Identity = 370/498 (74.30%), Postives = 425/498 (85.34%), Query Frame = 0


Query: 1   MEIASFSFL---LLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISM 60
           M I SFS L   LLLLLP+ + ILKHI S S + PLPPGP PWP+LGNLLQIGQNAHIS+
Sbjct: 1   MLIPSFSILLPSLLLLLPIFI-ILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISI 60

Query: 61  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSL 120
           TQF++IYGPLISLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQM PDRALHD  SL
Sbjct: 61  TQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSL 120

Query: 121 VFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVF 180
           VFSPECGD+WKNLRS+C+  LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+FVF
Sbjct: 121 VFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVF 180

Query: 181 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK 240
            +VFNTLSN IFS DL+ FVGDGF GIKG FK ++DL L PNLADFYPLLR FDLQGL+ 
Sbjct: 181 TSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKN 240

Query: 241 KAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGT 300
           KA IYKN++DS W ILIKERR IH +  SA+ DFLDVLIQSG+ ++QIN LI+E+LSAGT
Sbjct: 241 KADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGT 300

Query: 301 D--TTTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLH 360
           D  TTTVEWAM+E+LK++DI+N+VREE KK       IDES +SEL YLW+CVKETLRLH
Sbjct: 301 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLH 360

Query: 361 PPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--- 420
           PPAPFLLPR AP  CE+MGYS+PK +M+FVNVWGIGRDPS+WED Q+F P+RFD GC   
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 420

Query: 421 DADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGR 480
           + D KG+DYR+LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPNDE+ S LDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 480

Query: 481 LGITLQKEEPLKLLPKKR 489
           LG+TLQK E LKL+PK+R
Sbjct: 481 LGVTLQKYETLKLIPKRR 497

[Close]

BLAST of Carg20770 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 353.6 bits (906), Expect = 2.5e-97
Identity = 200/483 (41.41%), Postives = 289/483 (59.83%), Query Frame = 0


Query: 26  SSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAA 85
           SS K  P PPGP   P++GN+  +G+N H S    S  YGP++SLK G+   VV +SP A
Sbjct: 31  SSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEA 90

Query: 86  AAAILKTQDRLLSARYIFQMIPDRAL-HDNCSLVFSPECGDRWKNLRSICRAKLFTARAI 145
           A  +L+T D++LS+R     I  R++ HD  S+V+ P    RW+ LR +   +LF+ + I
Sbjct: 91  AREVLRTYDQILSSRTPTNSI--RSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRI 150

Query: 146 ESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFN 205
           E+  +LR  K+ E+V F+   S++   V+I    FIT  N +SN +FS DL  +  +   
Sbjct: 151 EATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNKSG 210

Query: 206 GIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRK--KAA------IYKNQIDSSWEILI 265
             +     V++    P+ A+F+P L   DLQG RK  KA       +++  ID+  ++  
Sbjct: 211 VFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFIDA--KLAE 270

Query: 266 KERRTIHSRGVSAATDFLDVLI------QSGYTNEQINYLIVEVLSAGTDT--TTVEWAM 325
           K  R  +S+ V    DF+DVL+      ++      I +L++++  AGTDT  +TVEWAM
Sbjct: 271 KSLRDTNSKDV-RERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDTNSSTVEWAM 330

Query: 326 AELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 385
           AELL+N + M + + E    I   G ++ES +S L YL   VKET RLHP AP L+PR A
Sbjct: 331 AELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLHPAAPLLVPRKA 390

Query: 386 PEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFG 445
               E++G+ VPK T +FVNVW IGRDP++WE+   FKPERF G D DL+G DY   PFG
Sbjct: 391 ESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYELTPFG 450

Query: 446 GGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLP 489
            GRR+CPGLP+A   VPL+LA+L+++F+W LPN   +  LD++   G+TL K  PL  +P
Sbjct: 451 AGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 508

[Close]

BLAST of Carg20770 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 336.7 bits (862), Expect = 3.2e-92
Identity = 189/492 (38.41%), Postives = 284/492 (57.72%), Query Frame = 0


Query: 16  LIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLKLGAQ 75
           LI    +   SS +   LPPGP   P++GN+ Q+G+N H S    + IYGP++SLK G  
Sbjct: 21  LISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCL 80

Query: 76  RLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICR 135
             VV +SP AA  +L+T D++LS R     I     H+  S+++ P    RW+ LR +  
Sbjct: 81  NSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSARWRMLRKLSV 140

Query: 136 AKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTLSNFIFSRDL 195
             +F+ +  E+  +LR KK+ E+V F+   S++   V+I    + TV N +SN +FS DL
Sbjct: 141 TLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDL 200

Query: 196 VGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKA--------AIYKNQ 255
             +     N  +      ++ A  P+ A+++P +   DLQG RK           +++  
Sbjct: 201 GSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGF 260

Query: 256 IDSSWEILIKERRTIHSRGVSAATDFLDVLI------QSGYTNEQINYLIVEVLSAGTDT 315
           +D+    + ++    +S+ VS   DFLD L+      ++   N  I +L++++ +AGTDT
Sbjct: 261 MDAK---IAEKSLGNYSKDVS-NRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDT 320

Query: 316 --TTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLHPP 375
             +T+EWAMAELL+N   M + + E  + +     + ES +S L YL   VKET RLHP 
Sbjct: 321 SSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPA 380

Query: 376 APFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKG 435
           AP L+PR A    E++G+ VPK T + VNVW IGRDPS+WE+P  F+PERF G D D+KG
Sbjct: 381 APLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDVKG 440

Query: 436 HDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQ 489
            DY   PFGGGRR+CPGLP+A   V L+LA+L+++F+W LPN   +  LD++   GITL 
Sbjct: 441 RDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLH 500

[Close]

BLAST of Carg20770 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 333.6 bits (854), Expect = 2.7e-91
Identity = 195/513 (38.01%), Postives = 298/513 (58.09%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQ-----PPLPPGPKPWPMLGNLLQIGQNAHI 60
           M+I S   LLLL   ++   L   ++ S +       LPPGP   P++GN+  +G++ H 
Sbjct: 1   MDIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHR 60

Query: 61  SMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRAL-HDN 120
           S  + S  YGP++SLKLG+   VV +SP AA  +L+T D++LSAR     +  R++ H +
Sbjct: 61  SFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAV--RSINHQD 120

Query: 121 CSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEI 180
            SLV+ P    RW+ LR +   +L + + IE+  +LR  K+ E+V F+   S +   V+I
Sbjct: 121 ASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDI 180

Query: 181 KEFVFITVFNTLSNFIFSRDLVGF-VGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFD 240
               FIT  N +SN +FS DL  +      NG++    +V+D A  P+ A+++P LR  D
Sbjct: 181 SRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLD 240

Query: 241 LQG--------LRKKAAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLI-----QS 300
           LQG          +   +++  ID+     I E+ + ++    +  DF+D L+     +S
Sbjct: 241 LQGNVKTFKVCTERLVRVFRGFIDAK----IAEKSSQNNPKDVSKNDFVDNLLDYKGDES 300

Query: 301 GYTNEQINYLIVEVLSAGTDT--TTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDES 360
             +   I +L++++ +AGTDT  +T+EWAM ELLKN   M + + E    I   G ++ES
Sbjct: 301 ELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEES 360

Query: 361 KLSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSI 420
            +S+L YL   VKET RLH P P L+PR A    E++G+ V K T + VNVW IGRDPS+
Sbjct: 361 DISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSV 420

Query: 421 WEDPQSFKPERFDGCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWC 480
           W++P  F+PERF G D D++G DY   PFG GRR+CPG+P+A   V L+LA+L+++F+W 
Sbjct: 421 WDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWK 480

Query: 481 LPNDENASTLDLNGRLGITLQKEEPLKLLPKKR 489
           LP    +  LD++   G+TL K  PL  +P K+
Sbjct: 481 LPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKK 507

[Close]

BLAST of Carg20770 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 329.7 bits (844), Expect = 3.9e-90
Identity = 179/480 (37.29%), Postives = 278/480 (57.92%), Query Frame = 0


Query: 33  LPPGPKPWPMLGNLLQI-GQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILK 92
           LPPGP PWP++GN+ Q+ G   H S+T+ S  +GP+++L++G+   VV SS   A  I K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 93  TQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLR 152
             D  L+ R I++ +      D    + + + G  W+ LR +C  + F  R +++ + +R
Sbjct: 96  KHDAALAGRKIYEAMKGGKSSDGS--LITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 153 RKKMNEMVEFLQ--SKQGS-VVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGHF 212
            + +++M+ F++   + G+  +++  + F+  FN + N +FSRDL+       +    H 
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFFYHT 215

Query: 213 KNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSSWEI---LIKERRTIHSRGV 272
             V++ A  PN+ADF+PLLR  D QG+R+K   +   ++ ++EI    I+ER  +  R  
Sbjct: 216 GKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFH---VEKAFEIAGEFIRERTEVREREK 275

Query: 273 S--AATDFLDVLIQ-----------SGYTNEQINYLIVEVLSAGTDTT--TVEWAMAELL 332
           S     D+LDVL++           S +++  IN ++ E+ +AGTDTT  T+EWA+AELL
Sbjct: 276 SDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELL 335

Query: 333 KNRDIMNRVREETKKAINGG--AIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPEA 392
            N   + +++ E +         + E  L  L YL   + ETLRLHPP PFL+P  A   
Sbjct: 336 HNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMST 395

Query: 393 CELMG-YSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERF--DGCDADLKGHDYRFLPFG 452
           C +   Y++PK T + VNVW IGRDP  W DP  FKPERF  D    D KG DY FLPFG
Sbjct: 396 CHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLPFG 455

Query: 453 GGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKLLP 486
            GRR+CP LP+A+  +PL + +++ +F+W L N  NA  +D+  R+GITL+K  PL+ +P
Sbjct: 456 SGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510

[Close]

BLAST of Carg20770 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 325.9 bits (834), Expect = 5.7e-89
Identity = 199/512 (38.87%), Postives = 292/512 (57.03%), Query Frame = 0


Query: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPL-----PPGPKPWPMLGNLLQIGQNAHI 60
           M+I S   L L+   ++  +L   ++ S++ P      PPGP   P++GN+  +G+N H 
Sbjct: 1   MDIISGQPLFLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHH 60

Query: 61  SMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNC 120
           S T  S  YGP++SLKLG    VV +S  A   +LKT D++LS RYI +       H   
Sbjct: 61  SFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNN-HHEF 120

Query: 121 SLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS--KQGSVVEIK 180
           S+ +      R++ LR +   +LF+ + I++  +LR KK+ E+V FL    ++   V+I 
Sbjct: 121 SVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDIS 180

Query: 181 EFVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQ 240
              F+T  N +SN +FS +L  +     +  +       +    P+LA+F+P +R  DLQ
Sbjct: 181 HVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQ 240

Query: 241 GLRKK--------AAIYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLI--QSGYTNE 300
           G  KK          +++   D+   I+ K  R++  + VS + DFLDVLI  Q G   E
Sbjct: 241 GNSKKMRESSGRLLQVFREFYDA--RIVEKSSRSV-EKDVS-SKDFLDVLIDLQQGDETE 300

Query: 301 ----QINYLIVEVLSAGTDT--TTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESK 360
               +I +L++++  AGTDT  +TVEWAMAELL N   M +V++E    I   G   ES 
Sbjct: 301 INIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESD 360

Query: 361 LSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIW 420
           +S+L YL   VKET RLHP APFLL R A    E++G++V K + + VNVW IGRDP +W
Sbjct: 361 ISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVW 420

Query: 421 EDPQSFKPERFDGCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCL 480
           E+P  F+PERF G + D+KG DY   PFG GRR+CPGLP+A   V L+LA+L++ FEW L
Sbjct: 421 ENPTHFEPERFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKL 480

Query: 481 PNDENASTLDLNGRLGITLQKEEPLKLLPKKR 489
           PN   +  LD+    G+T+ K  PL  +P K+
Sbjct: 481 PNGVGSEDLDMEETFGLTVHKTNPLLAVPLKK 507

The following BLAST results are available for this feature:

BLAST of Carg20770 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017133.1	5.4e-283	100.00	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma...	[more]
XP_022929108.1	7.6e-269	95.71	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata]	[more]
XP_022928791.1	1.7e-268	94.91	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata]	[more]
XP_022970204.1	2.6e-261	93.08	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima]	[more]
XP_023551257.1	4.4e-261	92.67	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ...	[more]

BLAST of Carg20770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FXW4	2.0e-118	46.12	Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3...	[more]
O64900	2.4e-116	44.61	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3...	[more]
O64899	4.1e-116	44.40	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif...	[more]
Q9SP06	3.2e-113	43.98	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum...	[more]
A8CDR5	7.5e-102	40.93	Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1	[more]

BLAST of Carg20770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ER52	3.7e-269	95.71	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O...	[more]
A0A6J1ELA6	8.2e-269	94.91	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O...	[more]
A0A6J1I4V7	1.3e-261	93.08	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=...	[more]
A0A5A7UIU3	1.5e-209	74.30	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]
A0A1S3CT14	2.5e-209	74.30	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656...	[more]

BLAST of Carg20770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45570.1	2.5e-97	41.41	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45550.1	3.2e-92	38.41	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]
AT2G45560.1	2.7e-91	38.01	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT3G52970.1	3.9e-90	37.29	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT1G33720.1	5.7e-89	38.87	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 61..80 score: 34.03 coord: 344..362 score: 32.62 coord: 302..328 score: 25.88 coord: 385..409 score: 36.89 coord: 421..431 score: 57.4 coord: 431..454 score: 31.32
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 293..310 score: 24.42 coord: 431..442 score: 28.4 coord: 345..356 score: 39.99 coord: 422..431 score: 59.57
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 34..474 e-value: 4.5E-92 score: 309.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..488 e-value: 2.3E-108 score: 364.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 34..488
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 11..467 e-value: 5.5E-17 score: 59.6
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 8..486
None	No IPR available	PANTHER	PTHR47956:SF23	(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKE	coord: 8..486
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 424..433

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20770-RA	Carg20770-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen