Carg18932 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg18932 vs. NCBI nr
Match: KAG7013938.1 (Metal tolerance protein C4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 900.2 bits (2325), Expect = 7.1e-258
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MLLWNREIAAVTIKKLDKAKSTLAQSCFDANILLATFWSPPLSPFFFLRFLCTSLPLSFF 60
           MLLWNREIAAVTIKKLDKAKSTLAQSCFDANILLATFWSPPLSPFFFLRFLCTSLPLSFF
Sbjct: 1   MLLWNREIAAVTIKKLDKAKSTLAQSCFDANILLATFWSPPLSPFFFLRFLCTSLPLSFF 60

Query: 61  DFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRSVLLSLISLDSKRARRLLI 120
           DFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRSVLLSLISLDSKRARRLLI
Sbjct: 61  DFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRSVLLSLISLDSKRARRLLI 120

Query: 121 HRYAFDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLGFGLPHEVMSCWQKLYTLLP 180
           HRYAFDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLGFGLPHEVMSCWQKLYTLLP
Sbjct: 121 HRYAFDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLGFGLPHEVMSCWQKLYTLLP 180

Query: 181 ILQISYGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCH 240
           ILQISYGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCH
Sbjct: 181 ILQISYGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCH 240

Query: 241 TSCQERCCCRGNETERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQYNSL 300
           TSCQERCCCRGNETERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQYNSL
Sbjct: 241 TSCQERCCCRGNETERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQYNSL 300

Query: 301 GVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLLILYVIAKASWLYEADF 360
           GVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLLILYVIAKASWLYEADF
Sbjct: 301 GVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLLILYVIAKASWLYEADF 360

Query: 361 NGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEI 420
           NGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEI
Sbjct: 361 NGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEI 420

Query: 421 QELVPGFRHVDIEAHSPTGSSP 443
           QELVPGFRHVDIEAHSPTGSSP
Sbjct: 421 QELVPGFRHVDIEAHSPTGSSP 442

BLAST of Carg18932 vs. NCBI nr
Match: XP_038898774.1 (metal tolerance protein C4 isoform X1 [Benincasa hispida])

HSP 1 Score: 446.0 bits (1146), Expect = 3.7e-121
Identity = 273/459 (59.48%), Postives = 295/459 (64.27%), Query Frame = 0

Query: 43  SPFFFLRFLCTSLPLSFFDFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRS 102
           S  F  RFLCTS P S FD KTPQHH Q+QPE DG  IPLIG  F S+RL+  SSCSRRS
Sbjct: 18  SSSFSFRFLCTSSPPSLFDSKTPQHHQQQQPECDGHRIPLIGTHFQSRRLSSLSSCSRRS 77

Query: 103 VLLSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVFS 162
           VLL LISLDS RAR LL H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVFS
Sbjct: 78  VLLGLISLDSNRARPLLNHHYAFHRGFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVFS 137

Query: 163 LNLG--FGLPHEVMSCW----------QKL--YTL-----LPILQISYGYSKERFVWSLI 222
           L  G  F     VM             Q L  Y L      P     YGYSKERFVWSLI
Sbjct: 138 LKFGVWFATSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLI 197

Query: 223 SAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC--------- 282
           SAVGIFC G+GATIVNGIQNLWTSQPPANIHYAAL   G                     
Sbjct: 198 SAVGIFCLGSGATIVNGIQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKKGAA 257

Query: 283 -----------RGNETERLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVVVGN 342
                      RG++   + V               +S   V+TTGNAIYDP+GS+VVGN
Sbjct: 258 AEGMKLRDYVWRGHDPTSVAVMTEDGAAVTGLIIAAASLVAVNTTGNAIYDPIGSIVVGN 317

Query: 343 LLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY--- 402
           LLGM             V IFLIQRNRHALIGRAMDENDMQ +LQFLKNDP++  LY   
Sbjct: 318 LLGM-------------VAIFLIQRNRHALIGRAMDENDMQKVLQFLKNDPVVDALYDCK 377

Query: 403 --VIAKASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGE 443
             VI    + ++A  DFNGVTVVQNYLSRTGREEWAREFRQAAK+ DDS LL+ M+NYGE
Sbjct: 378 SEVIGPGFYRFKAEIDFNGVTVVQNYLSRTGREEWAREFRQAAKSEDDSTLLKMMSNYGE 437

BLAST of Carg18932 vs. NCBI nr
Match: XP_038898775.1 (metal tolerance protein C4 isoform X2 [Benincasa hispida])

HSP 1 Score: 424.1 bits (1089), Expect = 1.5e-114
Identity = 257/442 (58.14%), Postives = 286/442 (64.71%), Query Frame = 0

Query: 43  SPFFFLRFLCTSLPLSFFDFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRS 102
           S  F  RFLCTS P S FD KTPQHH Q+QPE DG  IPLIG  F S+RL+  SSCSRRS
Sbjct: 18  SSSFSFRFLCTSSPPSLFDSKTPQHHQQQQPECDGHRIPLIGTHFQSRRLSSLSSCSRRS 77

Query: 103 VLLSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTA--SLCNFLV 162
           VLL LISLDS RAR LL H YA     F RA  VQRIEFNDYHS  ++     S+ +F  
Sbjct: 78  VLLGLISLDSNRARPLLNHHYAFHRGFFTRAKPVQRIEFNDYHSHVMLAEVVHSVADFAN 137

Query: 163 FSLNLGFGLPHEVMSCWQKLYTLLPILQISYGYSKERFVWSLISAVGIFCHGAGATIVNG 222
            +L L +GL     +         P     YGYSKERFVWSLISAVGIFC G+GATIVNG
Sbjct: 138 QAL-LAYGLSSSRRA---------PDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNG 197

Query: 223 IQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC--------------------RGNETE 282
           IQNLWTSQPPANIHYAAL   G                                RG++  
Sbjct: 198 IQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKKGAAAEGMKLRDYVWRGHDPT 257

Query: 283 RLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQYNSLGVFKFILH 342
            + V               +S   V+TTGNAIYDP+GS+VVGNLLGM             
Sbjct: 258 SVAVMTEDGAAVTGLIIAAASLVAVNTTGNAIYDPIGSIVVGNLLGM------------- 317

Query: 343 VDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY-----VIAKASWLYEA--DF 402
           V IFLIQRNRHALIGRAMDENDMQ +LQFLKNDP++  LY     VI    + ++A  DF
Sbjct: 318 VAIFLIQRNRHALIGRAMDENDMQKVLQFLKNDPVVDALYDCKSEVIGPGFYRFKAEIDF 377

Query: 403 NGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEI 443
           NGVTVVQNYLSRTGREEWAREFRQAAK+ DDS LL+ M+NYGEEVVT LGSEVDRL+KEI
Sbjct: 378 NGVTVVQNYLSRTGREEWAREFRQAAKSEDDSTLLKMMSNYGEEVVTALGSEVDRLEKEI 436

BLAST of Carg18932 vs. NCBI nr
Match: XP_022151077.1 (metal tolerance protein C4 [Momordica charantia])

HSP 1 Score: 421.0 bits (1081), Expect = 1.3e-113
Identity = 258/453 (56.95%), Postives = 284/453 (62.69%), Query Frame = 0

Query: 49  RFLCTSLPLSFFDFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRSVLLSLI 108
           RFLCTS P S  D   PQ H  +QPE D  G PL GA   S+RL+ A+SCSRRSVLL  I
Sbjct: 24  RFLCTSSPFSLLDPNIPQQHQPQQPECDSHGTPLFGAHCHSRRLSSAASCSRRSVLLGFI 83

Query: 109 SLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLG-- 168
           S DS R R L+ H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVFSL  G  
Sbjct: 84  SFDSNRGRPLVNHHYAFHRSFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVFSLKFGVW 143

Query: 169 FGLPHEVMSCW----------QKL--YTL-----LPILQISYGYSKERFVWSLISAVGIF 228
           F     VM             Q L  Y L      P     YGYSKERFVWSLISAVGIF
Sbjct: 144 FATSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIF 203

Query: 229 CHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC--------------- 288
           C G+GATIVNGIQNLWTSQPPANIHYAA    G                           
Sbjct: 204 CLGSGATIVNGIQNLWTSQPPANIHYAAFVIGGSLIIEGASLVVAIQAVKKGAAAEGMKL 263

Query: 289 -----RGNE-------TERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQY 348
                RG++       TE            +S   V+TTGNAIYDP+GS+VVGNLLGM  
Sbjct: 264 RDYVWRGHDPTSVAVMTEDGAAVTGLMIAAASLVAVNTTGNAIYDPIGSIVVGNLLGM-- 323

Query: 349 NSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY-----VIAK 408
                      V IFLIQRNRHALIGRAMDEND+Q +LQFLKNDP++  LY     VI  
Sbjct: 324 -----------VAIFLIQRNRHALIGRAMDENDVQKVLQFLKNDPVVDALYDCKSEVIGP 383

Query: 409 ASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGL 443
             + ++A  DFNGVTVVQNYL+RTGREEWAREFR+AAK +DDS LL+ M+NYGEEVVT L
Sbjct: 384 GFYRFKAEIDFNGVTVVQNYLNRTGREEWAREFREAAKKQDDSALLKMMSNYGEEVVTAL 443

BLAST of Carg18932 vs. NCBI nr
Match: XP_008462214.1 (PREDICTED: metal tolerance protein C4 isoform X1 [Cucumis melo])

HSP 1 Score: 418.7 bits (1075), Expect = 6.3e-113
Identity = 257/457 (56.24%), Postives = 287/457 (62.80%), Query Frame = 0

Query: 49  RFLCTSLPLSFFDFKTPQHHLQRQPER----DGCGIPLIGARFPSQRLAPASSCSRRSVL 108
           R LCTS P S FD KTPQ   Q+Q ++    D   IPLIG  F S+RL+  SSCSRRSVL
Sbjct: 31  RSLCTSSPPSLFDSKTPQQQQQQQQQQQQECDSLRIPLIGTHFQSRRLSSPSSCSRRSVL 90

Query: 109 LSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLN 168
           L LISLDS   R LL H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVFSL 
Sbjct: 91  LGLISLDSNPPRPLLNHHYAFYRGFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVFSLK 150

Query: 169 LGFGLP-----------HEVMSCWQK---LYTL-----LPILQISYGYSKERFVWSLISA 228
            G  L            H V     +    Y L      P     YGYSKERFVWSLISA
Sbjct: 151 FGVWLATSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISA 210

Query: 229 VGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC----------- 288
           VGIFC G+GATIVNGIQNLWTSQPPANIHYAAL   G                       
Sbjct: 211 VGIFCLGSGATIVNGIQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKKGAAAE 270

Query: 289 ---------RGNETERLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVVVGNLL 348
                    RG++   + V               +S   V+TTGNAIYDP+GS++VGNLL
Sbjct: 271 GMKLRDYIWRGHDPTSVAVMTEDGAAVTGLVIAAASLVAVNTTGNAIYDPIGSIIVGNLL 330

Query: 349 GMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY----- 408
           GM             V IFLIQRNRHALIGRAMDENDMQ +LQFLKNDP++  LY     
Sbjct: 331 GM-------------VAIFLIQRNRHALIGRAMDENDMQKVLQFLKNDPVVDALYDCKSE 390

Query: 409 VIAKASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEV 443
           VI    + ++A  DFNGV VVQNYL+RTGREEWAREFR+AAK++DDS LL+ M+NYGEEV
Sbjct: 391 VIGPGFYRFKAEIDFNGVMVVQNYLTRTGREEWAREFRRAAKSKDDSTLLKIMSNYGEEV 450

BLAST of Carg18932 vs. ExPASy Swiss-Prot
Match: Q8H1G3 (Metal tolerance protein C4 OS=Arabidopsis thaliana OX=3702 GN=MTPC4 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.7e-82
Identity = 195/376 (51.86%), Postives = 226/376 (60.11%), Query Frame = 0

Query: 121 HRYAFDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLGF---GLPHEVMS------- 180
           HR  F RA  V+RIE ND HSQR VTTA  CNFLVFSL  G       H +M+       
Sbjct: 93  HRNFFTRAKQVKRIEINDQHSQRAVTTALWCNFLVFSLKFGVWWTSSSHVIMAEVVHSVA 152

Query: 181 --CWQKL--YTL-----LPILQISYGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWT 240
               Q L  Y L      P     YGYSKERFVWSLISAVGIFC G+GATIVNG+QNLWT
Sbjct: 153 DFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNGVQNLWT 212

Query: 241 SQPPANIHYAALCFPGCC---------------HTSCQERCCC-----RGNE-------T 300
           S PP N+  AA+   G                   + QE         RG++       T
Sbjct: 213 SSPPPNMELAAVVIGGSFLIEGASLLVAIQSVKKGAAQEGMTIRDYIWRGHDPTSVAVMT 272

Query: 301 ERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQYNSLGVFKFILHVDIFLI 360
           E        +   +S   V  TGN IYDP+GS+VVGNLLGM             V IFLI
Sbjct: 273 EDGAAVAGLAIAAASLVAVRMTGNPIYDPIGSIVVGNLLGM-------------VAIFLI 332

Query: 361 QRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY-----VIAKASWLYEA--DFNGVTVV 420
           QRNRHALIGRAMD+ DM  +L+FL+ND ++  LY     VI   S+ ++A  DFNG  VV
Sbjct: 333 QRNRHALIGRAMDDQDMSKVLKFLRNDSVVDSLYDCKSEVIGPGSFRFKAEIDFNGQMVV 392

Query: 421 QNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEIQELVPG 443
           QNYL RTGREEWA+ FR+AAKN DDS +L  M+NYGEEVVT LGSEVDRL+KEIQELVPG
Sbjct: 393 QNYLKRTGREEWAKMFREAAKNGDDSAMLNIMSNYGEEVVTALGSEVDRLEKEIQELVPG 452

BLAST of Carg18932 vs. ExPASy Swiss-Prot
Match: Q5PQZ3 (Zinc transporter 9 OS=Danio rerio OX=7955 GN=slc30a9 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.4e-22
Identity = 88/288 (30.56%), Postives = 131/288 (45.49%), Query Frame = 0

Query: 186 YGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQE 245
           YG+S  R++ SLIS VGIF  GAG +  +GI  L   QP  ++ +A     G    S   
Sbjct: 305 YGFSNMRYIASLISGVGIFMMGAGLSWYHGIMGLLHPQPIESLLWAYCILAGSL-VSEGA 364

Query: 246 RCCCRGNETERLCVAWSRSRICSSYDRV-------------------------------- 305
                 NE ++     +R++  S Y+ V                                
Sbjct: 365 TLLVAINEIKKS----ARTQGLSFYEYVMQSRDPSTNVVLLEDAAAVLGVVLAAGCMGLT 424

Query: 306 STTGNAIYDPVGSVVVGNLLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQT 365
           S TGN  YD +GS+ VG LLG              V  FLI  N  AL+GR++    MQ 
Sbjct: 425 SLTGNPYYDSLGSLGVGTLLGT-------------VSAFLIYTNTEALLGRSIQAEHMQK 484

Query: 366 ILQFLKNDPLLIL--------YVIAKASWLYEADFNGVTVVQNYLSRTGREEWAREFRQA 425
           + +FL+NDP +            ++K  +  E DF+G  V ++YL +   E+   E +Q 
Sbjct: 485 LTEFLENDPAVRAIHDVKATDMGLSKVRFKAEVDFDGRVVTRSYLEKQDIEQILNEIQQV 544

Query: 426 AKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEIQELVPGFRHVDIE 434
              +   +L   M  +GE ++  LG+EVDRL+KE+++  P  RHVD+E
Sbjct: 545 ---KTPEELENFMLKHGENIIDTLGAEVDRLEKELKQRNPEVRHVDLE 571

BLAST of Carg18932 vs. ExPASy Swiss-Prot
Match: Q6PML9 (Zinc transporter 9 OS=Homo sapiens OX=9606 GN=SLC30A9 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.9e-22
Identity = 87/284 (30.63%), Postives = 129/284 (45.42%), Query Frame = 0

Query: 186 YGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQE 245
           YG+S  R++ SLIS VGIF  GAG +  +G+  L   QP  ++ +A     G    S   
Sbjct: 300 YGFSNMRYISSLISGVGIFMMGAGLSWYHGVMGLLHPQPIESLLWAYCILAGSL-VSEGA 359

Query: 246 RCCCRGNETERLCVAWSRS---RICSSYD-------------------------RVSTTG 305
                 NE  R   A   S    +  S D                           S TG
Sbjct: 360 TLLVAVNELRRNARAKGMSFYKYVMESRDPSTNVILLEDTAAVLGVIIAATCMGLTSITG 419

Query: 306 NAIYDPVGSVVVGNLLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQF 365
           N +YD +GS+ VG LLGM             V  FLI  N  AL+GR++    +Q + + 
Sbjct: 420 NPLYDSLGSLGVGTLLGM-------------VSAFLIYTNTEALLGRSIQPEQVQRLTEL 479

Query: 366 LKNDPLLILYVIAKASWL--------YEADFNGVTVVQNYLSRTGREEWAREFRQAAKNR 425
           L+NDP +      KA+ L         E DF+G  V ++YL +   ++  +E ++    +
Sbjct: 480 LENDPSVRAIHDVKATDLGLGKVRFKAEVDFDGRVVTRSYLEKQDFDQMLQEIQEV---K 539

Query: 426 DDSDLLETMANYGEEVVTGLGSEVDRLKKEIQELVPGFRHVDIE 434
              +L   M  +GE ++  LG+EVDRL+KE+++  P  RHVD+E
Sbjct: 540 TPEELETFMLKHGENIIDTLGAEVDRLEKELKKRNPEVRHVDLE 566

BLAST of Carg18932 vs. ExPASy Swiss-Prot
Match: Q5R4H0 (Zinc transporter 9 OS=Pongo abelii OX=9601 GN=SLC30A9 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.9e-22
Identity = 87/284 (30.63%), Postives = 129/284 (45.42%), Query Frame = 0

Query: 186 YGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQE 245
           YG+S  R++ SLIS VGIF  GAG +  +G+  L   QP  ++ +A     G    S   
Sbjct: 301 YGFSNMRYISSLISGVGIFMMGAGLSWYHGVMGLLHPQPIESLLWAYCILAGSL-VSEGA 360

Query: 246 RCCCRGNETERLCVAWSRS---RICSSYD-------------------------RVSTTG 305
                 NE  R   A   S    +  S D                           S TG
Sbjct: 361 TLLVAVNELRRNARAKGMSFYKYVMESRDPSTNVILLEDTAAVLGVIIAATCMGLTSITG 420

Query: 306 NAIYDPVGSVVVGNLLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQF 365
           N +YD +GS+ VG LLGM             V  FLI  N  AL+GR++    +Q + + 
Sbjct: 421 NPLYDSLGSLGVGTLLGM-------------VSAFLIYTNTEALLGRSIQPEQVQRLTEL 480

Query: 366 LKNDPLLILYVIAKASWL--------YEADFNGVTVVQNYLSRTGREEWAREFRQAAKNR 425
           L+NDP +      KA+ L         E DF+G  V ++YL +   ++  +E ++    +
Sbjct: 481 LENDPSVRAIHDVKATDLGLGKVRFKAEVDFDGRVVTRSYLEKQDFDQMLQEIQEV---K 540

Query: 426 DDSDLLETMANYGEEVVTGLGSEVDRLKKEIQELVPGFRHVDIE 434
              +L   M  +GE ++  LG+EVDRL+KE+++  P  RHVD+E
Sbjct: 541 TPEELETFMLKHGENIIDTLGAEVDRLEKELKKRNPEVRHVDLE 567

BLAST of Carg18932 vs. ExPASy Swiss-Prot
Match: Q6DCE3 (Zinc transporter 9 OS=Xenopus laevis OX=8355 GN=slc30a9 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 7.6e-21
Identity = 88/288 (30.56%), Postives = 129/288 (44.79%), Query Frame = 0

Query: 186 YGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQE 245
           YG++  R++ SLIS VGIF  GAG +  +GI  L   QP  ++ +A     G    S   
Sbjct: 291 YGFTNMRYIASLISGVGIFMMGAGLSWYHGIIGLLHPQPIESLLWAYCILAGSL-VSEGA 350

Query: 246 RCCCRGNETERLCVAWSRSRICSSYDRV-------------------------------- 305
                 NE  +     SR++  S Y  V                                
Sbjct: 351 TLLVAINEIRKS----SRAKGLSFYQYVMQSRDPSTNVVLMEDAAAVLGLVMAASCMGLT 410

Query: 306 STTGNAIYDPVGSVVVGNLLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQT 365
           S TGN +YD +GS+ VG LLG              V  FLI  N  ALIGR++  + +Q 
Sbjct: 411 SLTGNPLYDSLGSLGVGTLLGA-------------VSAFLIYTNTEALIGRSIQPDQVQR 470

Query: 366 ILQFLKNDPLLILYVIAKAS--------WLYEADFNGVTVVQNYLSRTGREEWAREFRQA 425
           + + L++DP +      KA+        +  E DF+G  V ++YL +   +    E RQ 
Sbjct: 471 LTELLESDPAVRAIHDVKATDMGMSKVRFKAEVDFDGRVVTRSYLEKQDIDLVLNEIRQV 530

Query: 426 AKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEIQELVPGFRHVDIE 434
              +   DL   M  +GE ++  LG+EVDRL+KE+++  P  RHVD+E
Sbjct: 531 ---KTAEDLEAFMLKHGENIIDTLGAEVDRLEKELKQRNPEVRHVDLE 557

BLAST of Carg18932 vs. ExPASy TrEMBL
Match: A0A6J1DCJ1 (metal tolerance protein C4 OS=Momordica charantia OX=3673 GN=LOC111019096 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 6.1e-114
Identity = 258/453 (56.95%), Postives = 284/453 (62.69%), Query Frame = 0

Query: 49  RFLCTSLPLSFFDFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRSVLLSLI 108
           RFLCTS P S  D   PQ H  +QPE D  G PL GA   S+RL+ A+SCSRRSVLL  I
Sbjct: 24  RFLCTSSPFSLLDPNIPQQHQPQQPECDSHGTPLFGAHCHSRRLSSAASCSRRSVLLGFI 83

Query: 109 SLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLG-- 168
           S DS R R L+ H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVFSL  G  
Sbjct: 84  SFDSNRGRPLVNHHYAFHRSFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVFSLKFGVW 143

Query: 169 FGLPHEVMSCW----------QKL--YTL-----LPILQISYGYSKERFVWSLISAVGIF 228
           F     VM             Q L  Y L      P     YGYSKERFVWSLISAVGIF
Sbjct: 144 FATSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIF 203

Query: 229 CHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC--------------- 288
           C G+GATIVNGIQNLWTSQPPANIHYAA    G                           
Sbjct: 204 CLGSGATIVNGIQNLWTSQPPANIHYAAFVIGGSLIIEGASLVVAIQAVKKGAAAEGMKL 263

Query: 289 -----RGNE-------TERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQY 348
                RG++       TE            +S   V+TTGNAIYDP+GS+VVGNLLGM  
Sbjct: 264 RDYVWRGHDPTSVAVMTEDGAAVTGLMIAAASLVAVNTTGNAIYDPIGSIVVGNLLGM-- 323

Query: 349 NSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY-----VIAK 408
                      V IFLIQRNRHALIGRAMDEND+Q +LQFLKNDP++  LY     VI  
Sbjct: 324 -----------VAIFLIQRNRHALIGRAMDENDVQKVLQFLKNDPVVDALYDCKSEVIGP 383

Query: 409 ASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGL 443
             + ++A  DFNGVTVVQNYL+RTGREEWAREFR+AAK +DDS LL+ M+NYGEEVVT L
Sbjct: 384 GFYRFKAEIDFNGVTVVQNYLNRTGREEWAREFREAAKKQDDSALLKMMSNYGEEVVTAL 443

BLAST of Carg18932 vs. ExPASy TrEMBL
Match: A0A1S3CGZ0 (metal tolerance protein C4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500625 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 3.0e-113
Identity = 257/457 (56.24%), Postives = 287/457 (62.80%), Query Frame = 0

Query: 49  RFLCTSLPLSFFDFKTPQHHLQRQPER----DGCGIPLIGARFPSQRLAPASSCSRRSVL 108
           R LCTS P S FD KTPQ   Q+Q ++    D   IPLIG  F S+RL+  SSCSRRSVL
Sbjct: 31  RSLCTSSPPSLFDSKTPQQQQQQQQQQQQECDSLRIPLIGTHFQSRRLSSPSSCSRRSVL 90

Query: 109 LSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLN 168
           L LISLDS   R LL H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVFSL 
Sbjct: 91  LGLISLDSNPPRPLLNHHYAFYRGFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVFSLK 150

Query: 169 LGFGLP-----------HEVMSCWQK---LYTL-----LPILQISYGYSKERFVWSLISA 228
            G  L            H V     +    Y L      P     YGYSKERFVWSLISA
Sbjct: 151 FGVWLATSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLISA 210

Query: 229 VGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC----------- 288
           VGIFC G+GATIVNGIQNLWTSQPPANIHYAAL   G                       
Sbjct: 211 VGIFCLGSGATIVNGIQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKKGAAAE 270

Query: 289 ---------RGNETERLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVVVGNLL 348
                    RG++   + V               +S   V+TTGNAIYDP+GS++VGNLL
Sbjct: 271 GMKLRDYIWRGHDPTSVAVMTEDGAAVTGLVIAAASLVAVNTTGNAIYDPIGSIIVGNLL 330

Query: 349 GMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY----- 408
           GM             V IFLIQRNRHALIGRAMDENDMQ +LQFLKNDP++  LY     
Sbjct: 331 GM-------------VAIFLIQRNRHALIGRAMDENDMQKVLQFLKNDPVVDALYDCKSE 390

Query: 409 VIAKASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEV 443
           VI    + ++A  DFNGV VVQNYL+RTGREEWAREFR+AAK++DDS LL+ M+NYGEEV
Sbjct: 391 VIGPGFYRFKAEIDFNGVMVVQNYLTRTGREEWAREFRRAAKSKDDSTLLKIMSNYGEEV 450

BLAST of Carg18932 vs. ExPASy TrEMBL
Match: A0A1L5J031 (Metal tolerance protein 7 OS=Cucumis sativus OX=3659 GN=MTP7 PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 8.8e-113
Identity = 261/460 (56.74%), Postives = 287/460 (62.39%), Query Frame = 0

Query: 49  RFLCTSLPLSFFDFKTPQHHLQR-------QPERDGCGIPLIGARFPSQRLAPASSCSRR 108
           R LCTS P S FD KTPQ   Q+       QPE D   IPLIG  F S+RL+  SSCSRR
Sbjct: 74  RSLCTSSPPSLFDSKTPQQQQQQQQQQQQHQPECDSYRIPLIGTYFQSRRLSSPSSCSRR 133

Query: 109 SVLLSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVF 168
           SVLL LISLDS   R  L H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVF
Sbjct: 134 SVLLGLISLDSNPPRPPLNHHYAFHRGFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVF 193

Query: 169 SLNLG--FGLPHEVMSCW----------QKL--YTL-----LPILQISYGYSKERFVWSL 228
           SL  G  F     VM             Q L  Y L      P     YGYSKERFVWSL
Sbjct: 194 SLKFGVWFATSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSL 253

Query: 229 ISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC-------- 288
           ISAVGIFC G+GATIVNGIQNLWTSQPPANIHYAAL   G                    
Sbjct: 254 ISAVGIFCLGSGATIVNGIQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKKGA 313

Query: 289 ------------RGNETERLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVVVG 348
                       RG++   + V               +S   V+TTGNAIYDP+GS++VG
Sbjct: 314 AAEGMKLRDYVWRGHDPTSVAVMTEDGAAVTGLIIAAASLVAVNTTGNAIYDPIGSIIVG 373

Query: 349 NLLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY-- 408
           NLLGM             V IFLIQRNRHALIGRAMDENDMQ +LQFLKNDP++  LY  
Sbjct: 374 NLLGM-------------VAIFLIQRNRHALIGRAMDENDMQKVLQFLKNDPVVDALYDC 433

Query: 409 ---VIAKASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYG 443
              VI    + ++A  DFNGV VVQNYL+RTGREEWAREFRQAAK++DDS LL+ M+NYG
Sbjct: 434 KSEVIGPGFYRFKAEIDFNGVMVVQNYLTRTGREEWAREFRQAAKSKDDSTLLKIMSNYG 493

BLAST of Carg18932 vs. ExPASy TrEMBL
Match: A0A6J1KD60 (metal tolerance protein C4-like OS=Cucurbita maxima OX=3661 GN=LOC111494540 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 259/459 (56.43%), Postives = 286/459 (62.31%), Query Frame = 0

Query: 43  SPFFFLRFLCTSLPLSFFDFKTPQHHLQRQPERDGCGIPLIGARFPSQRLAPASSCSRRS 102
           S  F  R +C S P S FD  TPQHH Q+Q E D  G P IG  F  + L  A++CSRRS
Sbjct: 18  SSSFSFRSICNSSPFSSFDSSTPQHHQQQQQECDSHGTPQIGTHFLLRPLPSATTCSRRS 77

Query: 103 VLLSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFLVFS 162
           VLL L SLDS R R LL H YA     F RA  VQRIEFNDYHSQR VTTA  CNFLVFS
Sbjct: 78  VLLGLNSLDSNRGRPLLNHHYAFHRGFFTRAKPVQRIEFNDYHSQRAVTTALWCNFLVFS 137

Query: 163 LNLG--FGLPHEVMSCW----------QKL--YTL-----LPILQISYGYSKERFVWSLI 222
           L  G  F     VM             Q L  Y L      P     YGYSKERFVWSLI
Sbjct: 138 LKFGVWFSTSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVWSLI 197

Query: 223 SAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC--------- 282
           SAVGIFC G+GATIVNGIQNLWTSQPPANIHYAAL   G                     
Sbjct: 198 SAVGIFCLGSGATIVNGIQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKKGAA 257

Query: 283 -----------RGNETERLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVVVGN 342
                      RG++   + V               +S   V+TTGNAIYDP+GS+VVGN
Sbjct: 258 AEGMKLRDYVWRGHDPTSVAVMTEDGAAVTGLIIAAASLVAVNTTGNAIYDPIGSIVVGN 317

Query: 343 LLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY--- 402
           LLGM             V IFLIQRNRHALIGRAMDENDMQ ILQFLKNDP++  LY   
Sbjct: 318 LLGM-------------VAIFLIQRNRHALIGRAMDENDMQKILQFLKNDPVVDALYDCK 377

Query: 403 --VIAKASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGE 443
             VI    + ++A  DFNGVTVVQNYL+RTGREEWA+EFRQAAK+++DS LL+ M+NYGE
Sbjct: 378 SEVIGPGFYRFKAEIDFNGVTVVQNYLNRTGREEWAKEFRQAAKDQNDSALLKMMSNYGE 437

BLAST of Carg18932 vs. ExPASy TrEMBL
Match: A0A6J1HJX8 (metal tolerance protein C4-like OS=Cucurbita moschata OX=3662 GN=LOC111464242 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.8e-110
Identity = 257/461 (55.75%), Postives = 286/461 (62.04%), Query Frame = 0

Query: 43  SPFFFLRFLCTSLPLSFFDFKTPQHHLQRQPER---DGCGIPLIGARFPSQRLAPASSCS 102
           S  F  R +C S P S FD  TPQHH Q+Q ++   DG G P IG  F  + L  A+SCS
Sbjct: 18  SSSFSFRSICNSSPFSSFDSGTPQHHQQQQQQQQECDGHGTPQIGTHFLLRPLPSATSCS 77

Query: 103 RRSVLLSLISLDSKRARRLLIHRYA-----FDRANSVQRIEFNDYHSQRVVTTASLCNFL 162
           RRSVLL L SLDS R R LL H YA     F RA  VQRIEFNDYHSQR VTTA  CNFL
Sbjct: 78  RRSVLLGLNSLDSNRGRPLLNHHYAFHRGFFTRAKPVQRIEFNDYHSQRAVTTALWCNFL 137

Query: 163 VFSLNLG--FGLPHEVMSCW----------QKL--YTL-----LPILQISYGYSKERFVW 222
           VFSL  G  F     VM             Q L  Y L      P     YGYSKERFVW
Sbjct: 138 VFSLKFGVWFSTSSHVMLAEVVHSVADFANQALLAYGLSSSRRAPDAIHPYGYSKERFVW 197

Query: 223 SLISAVGIFCHGAGATIVNGIQNLWTSQPPANIHYAALCFPGCCHTSCQERCCC------ 282
           SLISAVGIFC G+GATIVNGIQNLWTSQPPANIHYAAL   G                  
Sbjct: 198 SLISAVGIFCLGSGATIVNGIQNLWTSQPPANIHYAALVIGGSLIIEGASLVVAIQAVKK 257

Query: 283 --------------RGNETERLCVAWSRSR-------ICSSYDRVSTTGNAIYDPVGSVV 342
                         RG++   + V               +S   V+TTGNAIYDP+GS+V
Sbjct: 258 GAAAEGMKLRDYVWRGHDPTSVAVMTEDGAAVTGLIIAAASLVAVNTTGNAIYDPIGSIV 317

Query: 343 VGNLLGMQYNSLGVFKFILHVDIFLIQRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY 402
           VGNLLGM             V IFLIQRNRHALIGRAMDENDMQ +LQFLKNDP++  LY
Sbjct: 318 VGNLLGM-------------VAIFLIQRNRHALIGRAMDENDMQKVLQFLKNDPVVDALY 377

Query: 403 -----VIAKASWLYEA--DFNGVTVVQNYLSRTGREEWAREFRQAAKNRDDSDLLETMAN 442
                VI    + ++A  DFNGV VVQNYL+RTGREEWA+EFRQAAK+++DS LL+ M+N
Sbjct: 378 DCKSEVIGPGFYRFKAEIDFNGVAVVQNYLNRTGREEWAKEFRQAAKDQNDSALLKMMSN 437

BLAST of Carg18932 vs. TAIR 10
Match: AT1G51610.1 (Cation efflux family protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-83
Identity = 195/376 (51.86%), Postives = 226/376 (60.11%), Query Frame = 0

Query: 121 HRYAFDRANSVQRIEFNDYHSQRVVTTASLCNFLVFSLNLGF---GLPHEVMS------- 180
           HR  F RA  V+RIE ND HSQR VTTA  CNFLVFSL  G       H +M+       
Sbjct: 93  HRNFFTRAKQVKRIEINDQHSQRAVTTALWCNFLVFSLKFGVWWTSSSHVIMAEVVHSVA 152

Query: 181 --CWQKL--YTL-----LPILQISYGYSKERFVWSLISAVGIFCHGAGATIVNGIQNLWT 240
               Q L  Y L      P     YGYSKERFVWSLISAVGIFC G+GATIVNG+QNLWT
Sbjct: 153 DFANQALLAYGLSSSRRAPDALHPYGYSKERFVWSLISAVGIFCLGSGATIVNGVQNLWT 212

Query: 241 SQPPANIHYAALCFPGCC---------------HTSCQERCCC-----RGNE-------T 300
           S PP N+  AA+   G                   + QE         RG++       T
Sbjct: 213 SSPPPNMELAAVVIGGSFLIEGASLLVAIQSVKKGAAQEGMTIRDYIWRGHDPTSVAVMT 272

Query: 301 ERLCVAWSRSRICSSYDRVSTTGNAIYDPVGSVVVGNLLGMQYNSLGVFKFILHVDIFLI 360
           E        +   +S   V  TGN IYDP+GS+VVGNLLGM             V IFLI
Sbjct: 273 EDGAAVAGLAIAAASLVAVRMTGNPIYDPIGSIVVGNLLGM-------------VAIFLI 332

Query: 361 QRNRHALIGRAMDENDMQTILQFLKNDPLL-ILY-----VIAKASWLYEA--DFNGVTVV 420
           QRNRHALIGRAMD+ DM  +L+FL+ND ++  LY     VI   S+ ++A  DFNG  VV
Sbjct: 333 QRNRHALIGRAMDDQDMSKVLKFLRNDSVVDSLYDCKSEVIGPGSFRFKAEIDFNGQMVV 392

Query: 421 QNYLSRTGREEWAREFRQAAKNRDDSDLLETMANYGEEVVTGLGSEVDRLKKEIQELVPG 443
           QNYL RTGREEWA+ FR+AAKN DDS +L  M+NYGEEVVT LGSEVDRL+KEIQELVPG
Sbjct: 393 QNYLKRTGREEWAKMFREAAKNGDDSAMLNIMSNYGEEVVTALGSEVDRLEKEIQELVPG 452