Carg18874 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18874
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AMSH-like ubiquitin thioesterase 3
Location	Carg_Chr08: 7650107 .. 7654342 (-)
RNA-Seq Expression	Carg18874
Synteny	Carg18874

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCCCTCCTCCACGGCTGCACCTCATCATGTCGACATTAGAGTCCCAGTTTCAACTTCAATTTCACGGCCCCGCATTGATTCTTCGCGCTTAAGCAAATATATGTTATACGAGATTCCTATTCGTTCTTTGGAAGATTTCTACTGGGAGTTCATCAAATTGATTGCTAGCATCGATGAAGATCAACATCGATAGGGAGGCGCGGAAAGTTGGGGTTGATAATCGAATTCCCCTCCGGAATTACTATCGGATCGCCGATAATCTCCTAAAACAGGTAATTATTTCGAAAGCTTTCTTCTCGATTATTCTGATTGATTATTGATGCTGAAATGTTAATGTTTAATGTTTAATTTGAGGTTTTGTTAGTTTCCATTTCTTCCTCTGCGTGGTTGTTGAAGTGATTTTGATTGCAGTTTTTTTTACTGGTGGTTAGAATTTTCCTCCCGTTGTATTTTGATGTCATTGTGTTGTTTTCTTGAAATGGCAGGCTAATATTTATCGGGAGGAGAATAATATTGTGGATTTGTTCATTATACTTCTTAGGTTTTCAAGGTAAGGGCCTAGTTGATGCTTGTATTTTCCATTGCTCACGGATGTTGATTCTTGGTGTAGGAAACTCAATTCTCAACTTGTTTTTATTTTCTCAGCTTGGTTTCTGAAACTATACCACGGCATCGTGATTACCTAGCTTTTTTCCCAAAGGAAAAGATATTTTATAAGAAGGTGAGTTAAAACTCAATATCTCTGCTCTTCTGCAATGAATGGTATGTGAATGTTGTGTCATTTTATTTTATTTTATGTTGTCCTACCAGAAACTGTTGAGTGTAGTAGATGAACTTGAGTCCTTGAAGCCCGAATTTGATCGCCGAGTAAATGAACTGGAGAAAGTGTATGGACGGTCTCGTCTTCCTCCACCTGACGAGCAGGAAATAGTTTCGAACAGTCGACGGCCATCTCCCTATATGAACGGAAAATTTTCATTAATCTTGGATCAAAAGCAGGTATCAACTCTAAATGTTTGATATGATACTTTCTTCTCGTGTAACTATATCACTAATCAATGAAATTCCCACCAATTATTTAGAGTTTTTATTTACTTTTTGTCTTTTGCAGTTTTTATTTTTTGATTATTTACTGTTATCTTGTGATTTCACTCTACTGATACACAGCCTCCCCAATCCTCTTAATTCTCAGCATGCCAATTTACAACCACAATCTTCATTGAGGTATCATGACGACCGAGGTCTAGTTTCACAATCAAGTTCTGTAAGGATTGAGAATAAGTTCTCAAATCTGTGAGTATACATTTTGCCCATTTATTTCGTACATGTTTATATGAATCCATTAGCGGCGTAGTAGTCCCTACGCTTCATGATAATATAGGGAGATAAATCATTCCAATATATTATATTTTAGTGTAAGCTAGAAGAAGGTGATCTTGTAGTTCAAGTAGCTAGTTAGAGAGTAGAAGACAGAAAATAAGAAAGTATATTGACGACATCAGAGTTCTTCTGCATCTGGTCTCAAGATTGAATAGTAAGACAGTGTCTTTAGTATAAGGGCTTAAGAATACAATTCTAGGCGTGCTCATCTAGTCTCTAGTTCTTATGAACATGAACAAATGGATCTGATAATTAGTGCTGGGAATACACAGGTCTATCAATGTACCGCTTCCCAAACCCGAAACTTTGTCTAGACATTCTATTTTGGGTCCCAATGGTCTTCAGGGCCAATGGAAGGGACCGAGTACAGACAAGGTGAGTTTTGCGCATTCTTTTTTTTATGTTGTTTTCTTTTGTTTCTGTCTACTATGAAATCCATTCACAGGTTAATCTTTTTGTTCTGCCAGATTCTCTATCCAAGAAATCAGGATTATACTTCCGAAGATTCAAGGTCAGAGTCGTGTTTAACGTGTATTAGTATCTGAGGATTTACACTTTTTTTTTTGTCTTCTGTGTTTTGAGCAACCTTCCTGATAACGAAAGCTTAATGTGATTAATATTTTGATTTTGCTGTTTCAGTTTTTCTCTGATAATGTTTTCTTGAAAATTTATCTGGATCCTTCAAATTCTTGCAGATTGAATCAAATTGAACAATGTGATCTAGTGGCACAAAAAGATGGTGGTCCAGGAGGTGTTACGTCGATGATGGAATCGGTACTCTCGCTAGACGATGGCCTTTGGCCTCATCCTGCAGACATGTCTACTCCTCCATTGATAACAGAAGTAAGGGAAGATCCTTTCACATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAATATGCGACGATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAGTGGCAAAACAATCACAGGATGGACCTGAGTTCCAACATTTACACATTGTAGGCCTTCTCTCTTTGATTTTCGTTTATAACTTCCGAAAGCAATTAATTGATTTTCGTATATCTTCCGAAAGCAATTAATTGATTTTCGTATATCTTCCAATATGATATTGTTTACTTTTACTGTTTGGCGCAGCCAGTAAAAATGCTGGATGATTTCTTGAGATTGGCCCGAGAAAATACAAATAAGAATCTGGAGACTTGTGGTGTTTTAGCTGGCTCACTGGTAAGAACAAATTAATGGTTTATTGTAAATTTGTAAAGATTCTCTAGTTTTGTTCTCATACTTCATCATATATTTGCAGAAAAACAGGGTTTTTTACATATCTACGCTCATAGTCCCGAAGCAAGAATCAACATCAGATTCCGTAAGAATATAATTACTTTTCATCTGTTTCAAGTTAAAATTAGAAATTCCTTCCACGCCGTTGGTTTTCACTGCTCTTGAATGTTTGGTTTTCATTTTGACTGCTACTTGAAGTATGTTTGAGTTTTAATTGTTTTACATTCTACGTACATTCCTATGCTATTGGTTCATATATGGTTATAACTGACTCGAGGGTTACTTGATTGAGCGTTAGTTAGCTACCACTGTTGTATTCAATCAATACTCATTCTATCTTTTCTTTTTTTAAAAAAAATATTCAGTGCCAAACGTTGAATGAGGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATGTAAGCTTTCTGGCTCTTTATGCAAATAGAATTTGGGACACCAAATTATAAAAATTATGTTGATTTCTCGACTTCTCTGCATAAATTGTTTTGGTTTTGTCTTTAATATTCATTGCACTTTCTGACACTGATAGAACAGAAGATGAATTGCTGTAATATTATTGCTCGTGCAAGAATATCCCAATCTAGATAGACATTTGAGAGGCCTGACATTCTAACCTTACACCGAAAATGACCCTTTTCAAGATCAACCACATAAGCAATGAAACTACGTATAACCTTCATATGCGCAATACACTTACTTATTATTATCCTGAACAAGCATTCTTTATTAGTCAATAAGCTCTGTTTATTTCACTTCAGTTGACTTGCTATGTAGTGATGTCTTGAGATGGATGCGCTTAATAAAACTGAAAGGCAGAAGAGCTGGATGTAACATCCATTATATTTGTTCTTGAGAATATCGGAATTTTCGGCTTTTAACGTTTCTGTTTAGCTCGCATTATTTACTGGAATACGAGAAACTTCTGTTTTACATGTCTCTGATTCTTGTTTGATTTTCAGACACACCCATCACAAACGTGTTTCATGTCATCTGTGGATTTGCACACACATTACTCGTACCAGGTAATATAACAATTCTACTCTCTTTTGTTTTTTTGTTTTTTTATCCCTAGTGTCCTATCACAGAATATGTTTGGCTTTTTCAGATTATGCTACCAGAAGCTATTGCAATCGTCATGGCTCCTACGGATGAATCAAGGTGTTTTCCCCCTCCCCTCCATTCCTTCAACCACTCTTAAGTAGTATCATTTGTTTAACATATCCCAATGCTGTTGCAGTCCATATGGAATATTTCATCTGTCCGATCCAGGTGGTGTGTCGGTCATCCGCAACTGCCAACAGCGCGGGTTTCATCCCCATGAGGAGCCTGAAGATGGGAGTCCACTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACTCGAAGTTTGACGTTATCGATCTAAGGTAAGATCTTGATTGCTGACACTCTTTATAAGGGAACTTGTATAGCACCCCGAGAATGAGTACCTTAAACTTCGCCCCGACGAAAGCTCTGTAAATTTTGTAATTTGCAA

mRNA sequence

GCCCCCTCCTCCACGGCTGCACCTCATCATGTCGACATTAGAGTCCCAGTTTCAACTTCAATTTCACGGCCCCGCATTGATTCTTCGCGCTTAAGCAAATATATGTTATACGAGATTCCTATTCGTTCTTTGGAAGATTTCTACTGGGAGTTCATCAAATTGATTGCTAGCATCGATGAAGATCAACATCGATAGGGAGGCGCGGAAAGTTGGGGTTGATAATCGAATTCCCCTCCGGAATTACTATCGGATCGCCGATAATCTCCTAAAACAGGCTAATATTTATCGGGAGGAGAATAATATTGTGGATTTGTTCATTATACTTCTTAGGTTTTCAAGCTTGGTTTCTGAAACTATACCACGGCATCGTGATTACCTAGCTTTTTTCCCAAAGGAAAAGATATTTTATAAGAAGAAACTGTTGAGTGTAGTAGATGAACTTGAGTCCTTGAAGCCCGAATTTGATCGCCGAGTAAATGAACTGGAGAAAGTGTATGGACGGTCTCGTCTTCCTCCACCTGACGAGCAGGAAATAGTTTCGAACAGTCGACGGCCATCTCCCTATATGAACGGAAAATTTTCATTAATCTTGGATCAAAAGCAGCATGCCAATTTACAACCACAATCTTCATTGAGGTATCATGACGACCGAGGTCTAGTTTCACAATCAAGTTCTGTAAGGATTGAGAATAAGTTCTCAAATCTGTCTATCAATGTACCGCTTCCCAAACCCGAAACTTTGTCTAGACATTCTATTTTGGGTCCCAATGGTCTTCAGGGCCAATGGAAGGGACCGAGTACAGACAAGGTTAATCTTTTTGTTCTGCCAGATTCTCTATCCAAGAAATCAGGATTATACTTCCGAAGATTCAAGTTTTTCTCTGATAATGTTTTCTTGAAAATTTATCTGGATCCTTCAAATTCTTGCAGATTGAATCAAATTGAACAATGTGATCTAGTGGCACAAAAAGATGGTGGTCCAGGAGGTGTTACGTCGATGATGGAATCGGTACTCTCGCTAGACGATGGCCTTTGGCCTCATCCTGCAGACATGTCTACTCCTCCATTGATAACAGAAGTAAGGGAAGATCCTTTCACATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAATATGCGACGATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAGTGGCAAAACAATCACAGGATGGACCTGAGTTCCAACATTTACACATTCCAGTAAAAATGCTGGATGATTTCTTGAGATTGGCCCGAGAAAATACAAATAAGAATCTGGAGACTTGTGGTGTTTTAGCTGGCTCACTGAAAAACAGGGTTTTTTACATATCTACGCTCATAGTCCCGAAGCAAGAATCAACATCAGATTCCTGCCAAACGTTGAATGAGGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATACACACCCATCACAAACGTGTTTCATGTCATCTGTGGATTTGCACACACATTACTCGTACCAGATTATGCTACCAGAAGCTATTGCAATCGTCATGGCTCCTACGGATGAATCAAGTCCATATGGAATATTTCATCTGTCCGATCCAGGTGGTGTGTCGGTCATCCGCAACTGCCAACAGCGCGGGTTTCATCCCCATGAGGAGCCTGAAGATGGGAGTCCACTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACTCGAAGTTTGACGTTATCGATCTAAGGTAAGATCTTGATTGCTGACACTCTTTATAAGGGAACTTGTATAGCACCCCGAGAATGAGTACCTTAAACTTCGCCCCGACGAAAGCTCTGTAAATTTTGTAATTTGCAA

Coding sequence (CDS)

ATGAAGATCAACATCGATAGGGAGGCGCGGAAAGTTGGGGTTGATAATCGAATTCCCCTCCGGAATTACTATCGGATCGCCGATAATCTCCTAAAACAGGCTAATATTTATCGGGAGGAGAATAATATTGTGGATTTGTTCATTATACTTCTTAGGTTTTCAAGCTTGGTTTCTGAAACTATACCACGGCATCGTGATTACCTAGCTTTTTTCCCAAAGGAAAAGATATTTTATAAGAAGAAACTGTTGAGTGTAGTAGATGAACTTGAGTCCTTGAAGCCCGAATTTGATCGCCGAGTAAATGAACTGGAGAAAGTGTATGGACGGTCTCGTCTTCCTCCACCTGACGAGCAGGAAATAGTTTCGAACAGTCGACGGCCATCTCCCTATATGAACGGAAAATTTTCATTAATCTTGGATCAAAAGCAGCATGCCAATTTACAACCACAATCTTCATTGAGGTATCATGACGACCGAGGTCTAGTTTCACAATCAAGTTCTGTAAGGATTGAGAATAAGTTCTCAAATCTGTCTATCAATGTACCGCTTCCCAAACCCGAAACTTTGTCTAGACATTCTATTTTGGGTCCCAATGGTCTTCAGGGCCAATGGAAGGGACCGAGTACAGACAAGGTTAATCTTTTTGTTCTGCCAGATTCTCTATCCAAGAAATCAGGATTATACTTCCGAAGATTCAAGTTTTTCTCTGATAATGTTTTCTTGAAAATTTATCTGGATCCTTCAAATTCTTGCAGATTGAATCAAATTGAACAATGTGATCTAGTGGCACAAAAAGATGGTGGTCCAGGAGGTGTTACGTCGATGATGGAATCGGTACTCTCGCTAGACGATGGCCTTTGGCCTCATCCTGCAGACATGTCTACTCCTCCATTGATAACAGAAGTAAGGGAAGATCCTTTCACATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAATATGCGACGATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAGTGGCAAAACAATCACAGGATGGACCTGAGTTCCAACATTTACACATTCCAGTAAAAATGCTGGATGATTTCTTGAGATTGGCCCGAGAAAATACAAATAAGAATCTGGAGACTTGTGGTGTTTTAGCTGGCTCACTGAAAAACAGGGTTTTTTACATATCTACGCTCATAGTCCCGAAGCAAGAATCAACATCAGATTCCTGCCAAACGTTGAATGAGGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATACACACCCATCACAAACGTGTTTCATGTCATCTGTGGATTTGCACACACATTACTCGTACCAGATTATGCTACCAGAAGCTATTGCAATCGTCATGGCTCCTACGGATGAATCAAGTCCATATGGAATATTTCATCTGTCCGATCCAGGTGGTGTGTCGGTCATCCGCAACTGCCAACAGCGCGGGTTTCATCCCCATGAGGAGCCTGAAGATGGGAGTCCACTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACTCGAAGTTTGACGTTATCGATCTAAGGTAA

Protein sequence

MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSETIPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEIVSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSINVPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLITEVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR

Homology

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BLAST of Carg18874 vs. NCBI nr
Match: KAG7026341.1 (AMSH-like ubiquitin thioesterase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.7e-309
Identity = 528/528 (100.00%), Postives = 528/528 (100.00%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF
Sbjct: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
           LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT
Sbjct: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 528

[Close]

BLAST of Carg18874 vs. NCBI nr
Match: KAG6594001.1 (AMSH-like ubiquitin thioesterase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 977.2 bits (2525), Expect = 5.5e-281
Identity = 492/528 (93.18%), Postives = 496/528 (93.94%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           VPLPKPETLSRHSILGPNGLQGQWKGPSTDK+              LY R   + S++  
Sbjct: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKI--------------LYPRNQDYTSED-- 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
                      RLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT
Sbjct: 241 ----------SRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 502

[Close]

BLAST of Carg18874 vs. NCBI nr
Match: XP_022930498.1 (AMSH-like ubiquitin thioesterase 3 [Cucurbita moschata])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 489/528 (92.61%), Postives = 495/528 (93.75%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPY+NGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYVNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           VPLPKPETLSRHSILGPNGLQGQWKGPSTDK+              LY R   + S++  
Sbjct: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKI--------------LYPRNQDYTSED-- 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
                      RLNQIEQCDL AQKDGGPGGVTSMM+SVLSLDDGLWPHPADMSTPPLIT
Sbjct: 241 ----------SRLNQIEQCDLAAQKDGGPGGVTSMMKSVLSLDDGLWPHPADMSTPPLIT 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 502

[Close]

BLAST of Carg18874 vs. NCBI nr
Match: XP_023514277.1 (AMSH-like ubiquitin thioesterase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.1 bits (2509), Expect = 3.9e-279
Identity = 489/528 (92.61%), Postives = 495/528 (93.75%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPY+NGKFSLILDQKQHANLQPQSSLRY+DDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYVNGKFSLILDQKQHANLQPQSSLRYNDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           VPLPKPETLSRHSILGPNGLQGQWKGPSTDK+              LY R   + S++  
Sbjct: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKI--------------LYPRNQDYTSED-- 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
                      RLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLI 
Sbjct: 241 ----------SRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIA 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 502

[Close]

BLAST of Carg18874 vs. NCBI nr
Match: XP_023000049.1 (AMSH-like ubiquitin thioesterase 3 [Cucurbita maxima])

HSP 1 Score: 965.3 bits (2494), Expect = 2.1e-277
Identity = 487/528 (92.23%), Postives = 493/528 (93.37%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLP PDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPSPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPY+NGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYVNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           V LPKPETLSRHSILGPNGLQGQWKGPSTDK+              LY R   + S++  
Sbjct: 181 VSLPKPETLSRHSILGPNGLQGQWKGPSTDKI--------------LYPRNQDYTSED-- 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
                      RLNQIEQCDLVAQKDGGPGGVTSMM+SVLSLDDGLWPHPADMSTPPLIT
Sbjct: 241 ----------SRLNQIEQCDLVAQKDGGPGGVTSMMDSVLSLDDGLWPHPADMSTPPLIT 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDG EFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGSEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 502

[Close]

BLAST of Carg18874 vs. ExPASy Swiss-Prot
Match: Q5PNU3 (AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1 SV=2)

HSP 1 Score: 563.1 bits (1450), Expect = 3.2e-159
Identity = 309/537 (57.54%), Postives = 379/537 (70.58%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKI++++ AR++ VDNRIPLRNYYRIADNLL+QA+IYREE N+VDL+I+LLR+SSL+SET
Sbjct: 1   MKIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IP HRDY A  P+E++  +K+L +V++ELESLKPEF++ V++L +V   SR    D   +
Sbjct: 61  IPFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLNRVEDESRQDGSDLPVV 120

Query: 121 --VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLS 180
              S++    P     +S     K     QP  S  Y+++  L S S+  +I+ +F  LS
Sbjct: 121 SYSSDAVEWPPAHKASYSRPDINKPLPTSQP--SWTYNNN--LTSSSNRTQIDQQFQKLS 180

Query: 181 INVPLPKPETLSRHSILGPNGLQGQWKGPSTD-KVNLFVLPDSLSKKSGLYFRRFKFFSD 240
            +   P   TLSRHS LGPNGL+ Q   P ++ KV     P +    S          +D
Sbjct: 181 FDFLPPNQATLSRHSFLGPNGLKRQMVAPKSEIKVQ---YPSNTDWGS----------AD 240

Query: 241 NVFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPP 300
           N  L      S+S  LN             G     S + SVLSLDDG W   ++     
Sbjct: 241 NSGLIEAGPSSSSASLN-------------GDSQEVSTLNSVLSLDDGRWQRHSEAVNSQ 300

Query: 301 LITEVREDPFTLV--KQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPE----F 360
            I++  EDPF  V  KQPSPPPVLA+V QE A I PSKVADPRPG A  S +G E    +
Sbjct: 301 FISDATEDPFQFVGMKQPSPPPVLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSY 360

Query: 361 QHLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTL 420
           QHLH+PV+++DDFLRLAR NT +NLETCGVLAGSLKNRVF+I+TLI+PKQESTSDSCQTL
Sbjct: 361 QHLHVPVRIMDDFLRLARSNTERNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTL 420

Query: 421 NEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESS 480
           NEEEIFEVQDRLSLFPLGWIHTHP+QTCFMSSVDLHTHYSYQIMLPEA+AIVMAPTDES+
Sbjct: 421 NEEEIFEVQDRLSLFPLGWIHTHPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDEST 480

Query: 481 PYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           P+GIFHLSDP GVSVIRNCQQRGFHPHEE EDG+P+YEHCSHV +N   K++V+DLR
Sbjct: 481 PHGIFHLSDPSGVSVIRNCQQRGFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

[Close]

BLAST of Carg18874 vs. ExPASy Swiss-Prot
Match: Q8VYB5 (AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 2.0e-116
Identity = 249/536 (46.46%), Postives = 324/536 (60.45%), Query Frame = 0


Query: 3   INIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSETIP 62
           I+I   AR++GVDNRI L+ Y+RIADN+LKQANI+R E N++DL+++LLRFSSL  ETIP
Sbjct: 8   IDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIP 67

Query: 63  RHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNEL-EKVYGRSRLPPPDEQEIV 122
            HRDY       K + + +LL V+ ELE LKP   +R++EL  K+  R  +        +
Sbjct: 68  SHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDELYPKLKPRYNVQAHPANGSL 127

Query: 123 SNSRRPSPYMNGKFSLILDQKQHANLQ--PQSSLRYHDDRGLVSQSSSVRIENKFSNLSI 182
             S    P  N           HA ++  P  +  Y   RG     ++  +E +F  +S+
Sbjct: 128 GWSSAVKPSFN--------SYDHAKVRNPPGHNSGYMGSRG-QQFLNAAPLEERFRKMSV 187

Query: 183 NVPLPKPETLSRHSILGPNGLQGQWKGPSTD-KVNLFVLPDSLSKKSGLYFRRFKFFSDN 242
           N   P  ETLS+HSILGP GL  QW+ P  D KV     P ++   S +    F+   D+
Sbjct: 188 NF-RPNEETLSKHSILGPGGLSAQWQPPKYDTKVQ---YPSNID-FSPVVIPSFQQLVDS 247

Query: 243 VFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPL 302
               +  + SN      I +   VA  +      T  + S++S ++           P  
Sbjct: 248 --KPMITNGSNDEPEKPIVE-PSVASNEKIQKNYTEELSSMISFEE-----------PES 307

Query: 303 ITEVREDPFTLVKQPSPPPVLAKVQQEYATI------PPSKVADPRPGVAKQSQDGPEFQ 362
           + E       L++QPSPPPVLA+VQ     +      P   + +  P  + +S+   E  
Sbjct: 308 VNE-----NNLIRQPSPPPVLAEVQDLVPALCPEVREPECMIENSLPDESLRSESPLE-- 367

Query: 363 HLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLN 422
            LHI   M+D F+RLA+ NT KNLETCG+LAGSLKNR FYI+ LI+PKQESTSDSCQ  N
Sbjct: 368 -LHIATSMMDTFMRLAKSNTKKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATN 427

Query: 423 EEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSP 482
           EEEIFEVQD+ SLFPLGWIHTHP+Q+CFMSS+D+HTHYSYQIMLPEA+AIVMAP D S  
Sbjct: 428 EEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRN 487

Query: 483 YGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           +GIF L+ PGG++VIRNC +RGFH H  PEDG P+Y  C  V MNPN KFDVIDLR
Sbjct: 488 HGIFRLTTPGGMTVIRNCDRRGFHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

[Close]

BLAST of Carg18874 vs. ExPASy Swiss-Prot
Match: Q6NKP9 (AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 6.2e-70
Identity = 114/185 (61.62%), Postives = 148/185 (80.00%), Query Frame = 0


Query: 344 QSQDGPEFQHLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQES 403
           +S +    + +HI  ++L+DF  LARENT K+LETCG LA  L+  +FY++TLI+PKQES
Sbjct: 39  ESSEAKILRDVHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQES 98

Query: 404 TSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIV 463
           TS+SCQ +NE E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV
Sbjct: 99  TSNSCQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIV 158

Query: 464 MAPTDESSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFD 523
           +APTD S  YGIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N N +F+
Sbjct: 159 VAPTDSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFE 218

Query: 524 VIDLR 529
           + DLR
Sbjct: 219 IFDLR 223

[Close]

BLAST of Carg18874 vs. ExPASy Swiss-Prot
Match: Q9P371 (AMSH-like protease sst2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sst2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.7e-46
Identity = 98/204 (48.04%), Postives = 132/204 (64.71%), Query Frame = 0


Query: 332 SKVADPRPGVA------KQSQDGPEFQHLHIPVKMLDDFLRLARENTNKNLETCGVLAGS 391
           ++ ++PRP  A        ++ G   + +++P  +   FL + + NT KNLETCG+L G 
Sbjct: 235 TRTSEPRPKPAGTFKIHAYTEGGKPLRTIYLPKLLKKVFLDVVKPNTKKNLETCGILCGK 294

Query: 392 LKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVD 451
           L+   F+I+ L++P QE+TSD+C T +E  +FE QD+ +L  LGWIHTHP+QTCFMSSVD
Sbjct: 295 LRQNAFFITHLVIPLQEATSDTCGTTDEASLFEFQDKHNLLTLGWIHTHPTQTCFMSSVD 354

Query: 452 LHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSDPGGVSVIRNCQQRG-FHPHEEPEDG 511
           LHTH SYQ+MLPEAIAIVMAP+  +S  GIF L DP G+  I  C++ G FHPHE     
Sbjct: 355 LHTHCSYQLMLPEAIAIVMAPSKNTS--GIFRLLDPEGLQTIVKCRKPGLFHPHEGKV-- 414

Query: 512 SPLYEHCSHVLMNPNSKFDVIDLR 529
             +     HV    NSK  V+DLR
Sbjct: 415 YTMVAQPGHV-REINSKLQVVDLR 433

[Close]

BLAST of Carg18874 vs. ExPASy Swiss-Prot
Match: Q54Q40 (Probable ubiquitin thioesterase DG1039 OS=Dictyostelium discoideum OX=44689 GN=DG1039 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 5.3e-45
Identity = 84/172 (48.84%), Postives = 120/172 (69.77%), Query Frame = 0


Query: 359 KMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFE 418
           ++  +F+RLA  NT +++ETCG+L+G+L N VF I+T+I+PKQE T+D+C T+ E EIFE
Sbjct: 542 EVFQEFMRLAENNTKRSIETCGILSGTLSNDVFRITTIIIPKQEGTTDTCNTIEEHEIFE 601

Query: 419 VQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHL 478
            Q    L  LGWIHTHP+Q CF+S+VD+HTH SYQ +L EAIA+V++P    + +GIF L
Sbjct: 602 YQLENDLLTLGWIHTHPTQDCFLSAVDVHTHCSYQYLLQEAIAVVISPMANPN-FGIFRL 661

Query: 479 SDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHV--LMNPNSKFDVIDLR 529
           +DP G+  ++ C+ + FHPH  P +G P+Y    HV  +    S   V+DLR
Sbjct: 662 TDPPGLETVQKCKLKSFHPH-PPVNGIPIYTKVDHVDLIWGKKSDSKVVDLR 711

HSP 2 Score: 50.8 bits (120), Expect = 5.4e-05
Identity = 45/173 (26.01%), Postives = 79/173 (45.66%), Query Frame = 0


Query: 5   IDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSETIPRH 64
           + +    V VD    + +Y    +NL+KQA+IY+ E +I   +I  LRF  L+ E + +H
Sbjct: 17  VKKHVEGVEVDKNYSIFHYLSTCNNLVKQADIYKSEGDIERTYIYSLRFCILIFEKLQKH 76

Query: 65  RDY-LAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEIVSN 124
            D+    F K +   K+K    + ELE LK         L+K Y R +    +E++ +  
Sbjct: 77  PDFNKESFTKSRNEIKRKAELKLKELEGLK-------ETLKKGYERIQHKKEEERKRIER 136

Query: 125 SRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSN 177
            +        +  L L+++Q   L+ +   R  +D  L S+   ++    F N
Sbjct: 137 EKEKERIFKQE-KLKLEREQQL-LREEEEQRKREDLELESEIQRLKEVEDFEN 180

[Close]

BLAST of Carg18874 vs. ExPASy TrEMBL
Match: A0A6J1EVD2 (AMSH-like ubiquitin thioesterase 3 OS=Cucurbita moschata OX=3662 GN=LOC111436935 PE=3 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 6.5e-280
Identity = 489/528 (92.61%), Postives = 495/528 (93.75%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPY+NGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYVNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           VPLPKPETLSRHSILGPNGLQGQWKGPSTDK+              LY R   + S++  
Sbjct: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKI--------------LYPRNQDYTSED-- 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
                      RLNQIEQCDL AQKDGGPGGVTSMM+SVLSLDDGLWPHPADMSTPPLIT
Sbjct: 241 ----------SRLNQIEQCDLAAQKDGGPGGVTSMMKSVLSLDDGLWPHPADMSTPPLIT 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 502

[Close]

BLAST of Carg18874 vs. ExPASy TrEMBL
Match: A0A6J1KIT9 (AMSH-like ubiquitin thioesterase 3 OS=Cucurbita maxima OX=3661 GN=LOC111494356 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.0e-277
Identity = 487/528 (92.23%), Postives = 493/528 (93.37%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLP PDEQEI
Sbjct: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPSPDEQEI 120

Query: 121 VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180
           VSNSRRPSPY+NGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN
Sbjct: 121 VSNSRRPSPYVNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLSIN 180

Query: 181 VPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFSDNVF 240
           V LPKPETLSRHSILGPNGLQGQWKGPSTDK+              LY R   + S++  
Sbjct: 181 VSLPKPETLSRHSILGPNGLQGQWKGPSTDKI--------------LYPRNQDYTSED-- 240

Query: 241 LKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPLIT 300
                      RLNQIEQCDLVAQKDGGPGGVTSMM+SVLSLDDGLWPHPADMSTPPLIT
Sbjct: 241 ----------SRLNQIEQCDLVAQKDGGPGGVTSMMDSVLSLDDGLWPHPADMSTPPLIT 300

Query: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPEFQHLHIPVKM 360
           EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDG EFQHLHIPVKM
Sbjct: 301 EVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGSEFQHLHIPVKM 360

Query: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420
           LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ
Sbjct: 361 LDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEEIFEVQ 420

Query: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480
           DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD
Sbjct: 421 DRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGIFHLSD 480

Query: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR
Sbjct: 481 PGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 502

[Close]

BLAST of Carg18874 vs. ExPASy TrEMBL
Match: A0A0A0KG71 (MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G311020 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.5e-252
Identity = 443/533 (83.11%), Postives = 467/533 (87.62%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKV VDNRIPLRNY+RIADNLLKQANIYREENN+VDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDY AFFP EKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGR RLPPPDEQ  
Sbjct: 61  IPRHRDYPAFFPNEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQGS 120

Query: 121 VSNSRRPS----PYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSN 180
           VSNS RPS    PY+NG +SL+LDQKQH + +PQSS RY DDRG VS+SSS+R+ENKFS 
Sbjct: 121 VSNSFRPSPKEQPYVNGNYSLMLDQKQHVHSRPQSSRRYSDDRGQVSESSSLRLENKFST 180

Query: 181 LSINVPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFS 240
           LS+NVPLPKPETLSRHSILGP+GLQGQWKGPSTDK+   +  D   + S           
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIQYPINQDFTLEDS----------- 240

Query: 241 DNVFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTP 300
                          RLNQ E CDLVA KD GPGGV S+MESVLSLDDG WPHPADMS P
Sbjct: 241 ---------------RLNQNEHCDLVAHKDSGPGGVMSVMESVLSLDDGRWPHPADMSIP 300

Query: 301 PLITEVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPE-FQHLH 360
           PLITEVREDPF LVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVA+QSQD  E FQ LH
Sbjct: 301 PLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVARQSQDESESFQRLH 360

Query: 361 IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEE 420
           IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLI+PKQESTSDSCQTLNEEE
Sbjct: 361 IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEE 420

Query: 421 IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGI 480
           IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGI
Sbjct: 421 IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGI 480

Query: 481 FHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           FHLSDPGGVSVIRNCQQRGFHPHEEP+DGSPLYEHCSHV+MNPN+KFDV+DLR
Sbjct: 481 FHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVMMNPNAKFDVMDLR 507

[Close]

BLAST of Carg18874 vs. ExPASy TrEMBL
Match: A0A6J1L013 (AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499142 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.7e-251
Identity = 452/533 (84.80%), Postives = 466/533 (87.43%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKV VDNRIPLRNY+RIADNLLKQA+IYREENN+VDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDY AFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKV GR RLPP DEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPPSDEQGG 120

Query: 121 VSNSRRPS----PYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSN 180
           VSNS RPS    PY NG +SL+LDQKQHAN QPQSS RYHD RG VSQSS  RIEN+ SN
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLVLDQKQHANSQPQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSINVPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFS 240
           LS+NVPLPKPETLSRHSILGP+G QGQWKGPSTDK++              Y R   F  
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIH--------------YPRNQDF-- 240

Query: 241 DNVFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTP 300
                     P +S RLNQIEQCDLVA KD GPGGV SMMESVLSLDDG WPHPAD+S  
Sbjct: 241 ---------TPLDS-RLNQIEQCDLVAHKDSGPGGVMSMMESVLSLDDGRWPHPADISIT 300

Query: 301 PLITEVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPE-FQHLH 360
           PLITEVREDPF LVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQS D  E FQ LH
Sbjct: 301 PLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSLDESESFQRLH 360

Query: 361 IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEE 420
           IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLI+PKQESTSDSCQTLNEEE
Sbjct: 361 IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEE 420

Query: 421 IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGI 480
           IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGI
Sbjct: 421 IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGI 480

Query: 481 FHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           FHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPN+KFDVIDLR
Sbjct: 481 FHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNAKFDVIDLR 507

[Close]

BLAST of Carg18874 vs. ExPASy TrEMBL
Match: A0A5D3BT78 (AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00210 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.2e-249
Identity = 445/533 (83.49%), Postives = 466/533 (87.43%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKINIDREARKV VDNRIPLRNY+RIADNLLKQANIYREENN+VDLFIILLRFSSLVSET
Sbjct: 54  MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 113

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IPRHRDY AFFPKEKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGR RLP PDEQ  
Sbjct: 114 IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 173

Query: 121 VSNSRRPS----PYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSN 180
           VSNS RPS    PY NG +SL+LDQKQH N +PQSS RY DDRG VSQSSS+R+ENKFS 
Sbjct: 174 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 233

Query: 181 LSINVPLPKPETLSRHSILGPNGLQGQWKGPSTDKVNLFVLPDSLSKKSGLYFRRFKFFS 240
           +S+NVPLPKPETLSRHSILGP+GLQ QW GPSTDK+               Y R   F  
Sbjct: 234 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQ--------------YPRNQDF-- 293

Query: 241 DNVFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTP 300
                     P +S RLNQ E CDLVA KD G GGV SMMESVLSLDDG WP+PADMSTP
Sbjct: 294 ---------TPEDS-RLNQSEHCDLVAHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTP 353

Query: 301 PLITEVREDPFTLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPE-FQHLH 360
           PLITEVREDPF LVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVA+QSQD  E FQ LH
Sbjct: 354 PLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVARQSQDESESFQRLH 413

Query: 361 IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLNEEE 420
           IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNR+FYISTLI+PKQESTSDSCQTLNEEE
Sbjct: 414 IPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEE 473

Query: 421 IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSPYGI 480
           IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGI
Sbjct: 474 IFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGI 533

Query: 481 FHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           FHLSDPGGVSVIRNCQQRGFHPHEEP+DGSPLYEHCSHV+MNPN+KFDVIDLR
Sbjct: 534 FHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVMMNPNAKFDVIDLR 560

[Close]

BLAST of Carg18874 vs. TAIR 10
Match: AT4G16144.1 (associated molecule with the SH3 domain of STAM 3 )

HSP 1 Score: 563.1 bits (1450), Expect = 2.3e-160
Identity = 309/537 (57.54%), Postives = 379/537 (70.58%), Query Frame = 0


Query: 1   MKINIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSET 60
           MKI++++ AR++ VDNRIPLRNYYRIADNLL+QA+IYREE N+VDL+I+LLR+SSL+SET
Sbjct: 1   MKIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISET 60

Query: 61  IPRHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRSRLPPPDEQEI 120
           IP HRDY A  P+E++  +K+L +V++ELESLKPEF++ V++L +V   SR    D   +
Sbjct: 61  IPFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLNRVEDESRQDGSDLPVV 120

Query: 121 --VSNSRRPSPYMNGKFSLILDQKQHANLQPQSSLRYHDDRGLVSQSSSVRIENKFSNLS 180
              S++    P     +S     K     QP  S  Y+++  L S S+  +I+ +F  LS
Sbjct: 121 SYSSDAVEWPPAHKASYSRPDINKPLPTSQP--SWTYNNN--LTSSSNRTQIDQQFQKLS 180

Query: 181 INVPLPKPETLSRHSILGPNGLQGQWKGPSTD-KVNLFVLPDSLSKKSGLYFRRFKFFSD 240
            +   P   TLSRHS LGPNGL+ Q   P ++ KV     P +    S          +D
Sbjct: 181 FDFLPPNQATLSRHSFLGPNGLKRQMVAPKSEIKVQ---YPSNTDWGS----------AD 240

Query: 241 NVFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPP 300
           N  L      S+S  LN             G     S + SVLSLDDG W   ++     
Sbjct: 241 NSGLIEAGPSSSSASLN-------------GDSQEVSTLNSVLSLDDGRWQRHSEAVNSQ 300

Query: 301 LITEVREDPFTLV--KQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPE----F 360
            I++  EDPF  V  KQPSPPPVLA+V QE A I PSKVADPRPG A  S +G E    +
Sbjct: 301 FISDATEDPFQFVGMKQPSPPPVLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSY 360

Query: 361 QHLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTL 420
           QHLH+PV+++DDFLRLAR NT +NLETCGVLAGSLKNRVF+I+TLI+PKQESTSDSCQTL
Sbjct: 361 QHLHVPVRIMDDFLRLARSNTERNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTL 420

Query: 421 NEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESS 480
           NEEEIFEVQDRLSLFPLGWIHTHP+QTCFMSSVDLHTHYSYQIMLPEA+AIVMAPTDES+
Sbjct: 421 NEEEIFEVQDRLSLFPLGWIHTHPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDEST 480

Query: 481 PYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           P+GIFHLSDP GVSVIRNCQQRGFHPHEE EDG+P+YEHCSHV +N   K++V+DLR
Sbjct: 481 PHGIFHLSDPSGVSVIRNCQQRGFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

[Close]

BLAST of Carg18874 vs. TAIR 10
Match: AT1G48790.1 (associated molecule with the SH3 domain of STAM 1 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.4e-117
Identity = 249/536 (46.46%), Postives = 324/536 (60.45%), Query Frame = 0


Query: 3   INIDREARKVGVDNRIPLRNYYRIADNLLKQANIYREENNIVDLFIILLRFSSLVSETIP 62
           I+I   AR++GVDNRI L+ Y+RIADN+LKQANI+R E N++DL+++LLRFSSL  ETIP
Sbjct: 8   IDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIP 67

Query: 63  RHRDYLAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNEL-EKVYGRSRLPPPDEQEIV 122
            HRDY       K + + +LL V+ ELE LKP   +R++EL  K+  R  +        +
Sbjct: 68  SHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDELYPKLKPRYNVQAHPANGSL 127

Query: 123 SNSRRPSPYMNGKFSLILDQKQHANLQ--PQSSLRYHDDRGLVSQSSSVRIENKFSNLSI 182
             S    P  N           HA ++  P  +  Y   RG     ++  +E +F  +S+
Sbjct: 128 GWSSAVKPSFN--------SYDHAKVRNPPGHNSGYMGSRG-QQFLNAAPLEERFRKMSV 187

Query: 183 NVPLPKPETLSRHSILGPNGLQGQWKGPSTD-KVNLFVLPDSLSKKSGLYFRRFKFFSDN 242
           N   P  ETLS+HSILGP GL  QW+ P  D KV     P ++   S +    F+   D+
Sbjct: 188 NF-RPNEETLSKHSILGPGGLSAQWQPPKYDTKVQ---YPSNID-FSPVVIPSFQQLVDS 247

Query: 243 VFLKIYLDPSNSCRLNQIEQCDLVAQKDGGPGGVTSMMESVLSLDDGLWPHPADMSTPPL 302
               +  + SN      I +   VA  +      T  + S++S ++           P  
Sbjct: 248 --KPMITNGSNDEPEKPIVE-PSVASNEKIQKNYTEELSSMISFEE-----------PES 307

Query: 303 ITEVREDPFTLVKQPSPPPVLAKVQQEYATI------PPSKVADPRPGVAKQSQDGPEFQ 362
           + E       L++QPSPPPVLA+VQ     +      P   + +  P  + +S+   E  
Sbjct: 308 VNE-----NNLIRQPSPPPVLAEVQDLVPALCPEVREPECMIENSLPDESLRSESPLE-- 367

Query: 363 HLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQESTSDSCQTLN 422
            LHI   M+D F+RLA+ NT KNLETCG+LAGSLKNR FYI+ LI+PKQESTSDSCQ  N
Sbjct: 368 -LHIATSMMDTFMRLAKSNTKKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATN 427

Query: 423 EEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDESSP 482
           EEEIFEVQD+ SLFPLGWIHTHP+Q+CFMSS+D+HTHYSYQIMLPEA+AIVMAP D S  
Sbjct: 428 EEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRN 487

Query: 483 YGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDLR 529
           +GIF L+ PGG++VIRNC +RGFH H  PEDG P+Y  C  V MNPN KFDVIDLR
Sbjct: 488 HGIFRLTTPGGMTVIRNCDRRGFHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

[Close]

BLAST of Carg18874 vs. TAIR 10
Match: AT1G10600.1 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 266.5 bits (680), Expect = 4.4e-71
Identity = 114/185 (61.62%), Postives = 148/185 (80.00%), Query Frame = 0


Query: 344 QSQDGPEFQHLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQES 403
           +S +    + +HI  ++L+DF  LARENT K+LETCG LA  L+  +FY++TLI+PKQES
Sbjct: 39  ESSEAKILRDVHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQES 98

Query: 404 TSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIV 463
           TS+SCQ +NE E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV
Sbjct: 99  TSNSCQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIV 158

Query: 464 MAPTDESSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFD 523
           +APTD S  YGIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N N +F+
Sbjct: 159 VAPTDSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFE 218

Query: 524 VIDLR 529
           + DLR
Sbjct: 219 IFDLR 223

[Close]

BLAST of Carg18874 vs. TAIR 10
Match: AT1G10600.3 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 263.1 bits (671), Expect = 4.9e-70
Identity = 115/185 (62.16%), Postives = 149/185 (80.54%), Query Frame = 0


Query: 344 QSQDGPEFQHLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIVPKQES 403
           +S +    + +HI  ++L+DF  LARENT K+LETCG LA  L+  +FY++TLI+PKQES
Sbjct: 39  ESSEAKILRDVHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQES 98

Query: 404 TSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIV 463
           TS+SCQ +NE E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV
Sbjct: 99  TSNSCQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIV 158

Query: 464 MAPTDESSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFD 523
           +APTD SS YGIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N N +F+
Sbjct: 159 VAPTD-SSNYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFE 218

Query: 524 VIDLR 529
           + DLR
Sbjct: 219 IFDLR 222

[Close]

BLAST of Carg18874 vs. TAIR 10
Match: AT1G10600.2 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 194.9 bits (494), Expect = 1.6e-49
Identity = 80/121 (66.12%), Postives = 100/121 (82.64%), Query Frame = 0


Query: 408 CQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPT 467
           CQ +NE E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APT
Sbjct: 46  CQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPT 105

Query: 468 DESSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPEDGSPLYEHCSHVLMNPNSKFDVIDL 527
           D S  YGIF L+DPGG+ V+R C + GFHPH+EPEDG+P+YEHCS+V  N N +F++ DL
Sbjct: 106 DSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDL 165

Query: 528 R 529
           R
Sbjct: 166 R 166

The following BLAST results are available for this feature:

BLAST of Carg18874 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026341.1	3.7e-309	100.00	AMSH-like ubiquitin thioesterase 3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6594001.1	5.5e-281	93.18	AMSH-like ubiquitin thioesterase 3, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022930498.1	1.3e-279	92.61	AMSH-like ubiquitin thioesterase 3 [Cucurbita moschata]	[more]
XP_023514277.1	3.9e-279	92.61	AMSH-like ubiquitin thioesterase 3 [Cucurbita pepo subsp. pepo]	[more]
XP_023000049.1	2.1e-277	92.23	AMSH-like ubiquitin thioesterase 3 [Cucurbita maxima]	[more]

BLAST of Carg18874 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5PNU3	3.2e-159	57.54	AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1...	[more]
Q8VYB5	2.0e-116	46.46	AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2...	[more]
Q6NKP9	6.2e-70	61.62	AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2...	[more]
Q9P371	3.7e-46	48.04	AMSH-like protease sst2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
Q54Q40	5.3e-45	48.84	Probable ubiquitin thioesterase DG1039 OS=Dictyostelium discoideum OX=44689 GN=D...	[more]

BLAST of Carg18874 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EVD2	6.5e-280	92.61	AMSH-like ubiquitin thioesterase 3 OS=Cucurbita moschata OX=3662 GN=LOC111436935...	[more]
A0A6J1KIT9	1.0e-277	92.23	AMSH-like ubiquitin thioesterase 3 OS=Cucurbita maxima OX=3661 GN=LOC111494356 P...	[more]
A0A0A0KG71	7.5e-252	83.11	MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G311020 PE=3 SV...	[more]
A0A6J1L013	1.7e-251	84.80	AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5D3BT78	1.2e-249	83.49	AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Carg18874 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16144.1	2.3e-160	57.54	associated molecule with the SH3 domain of STAM 3	[more]
AT1G48790.1	1.4e-117	46.46	associated molecule with the SH3 domain of STAM 1	[more]
AT1G10600.1	4.4e-71	61.62	associated molecule with the SH3 domain of STAM 2	[more]
AT1G10600.3	4.9e-70	62.16	associated molecule with the SH3 domain of STAM 2	[more]
AT1G10600.2	1.6e-49	66.12	associated molecule with the SH3 domain of STAM 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 86..106
None	No IPR available	GENE3D	3.40.140.10	Cytidine Deaminase, domain 2	coord: 337..528 e-value: 1.4E-71 score: 241.8
None	No IPR available	GENE3D	1.20.58.80		coord: 3..119 e-value: 2.7E-32 score: 113.2
None	No IPR available	PANTHER	PTHR12947:SF18	AMSH-LIKE UBIQUITIN THIOESTERASE 3	coord: 245..528
None	No IPR available	PANTHER	PTHR12947	AMSH-LIKE PROTEASE	coord: 245..528
None	No IPR available	PANTHER	PTHR12947	AMSH-LIKE PROTEASE	coord: 2..210
None	No IPR available	PANTHER	PTHR12947:SF18	AMSH-LIKE UBIQUITIN THIOESTERASE 3	coord: 2..210
None	No IPR available	SUPERFAMILY	102712	JAB1/MPN domain	coord: 353..484
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	SMART	SM00232	pad1_6	coord: 353..481 e-value: 3.6E-13 score: 59.8
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	PFAM	PF01398	JAB	coord: 351..457 e-value: 4.3E-16 score: 59.0
IPR015063	USP8 dimerisation domain	PFAM	PF08969	USP8_dimer	coord: 9..99 e-value: 4.4E-11 score: 42.9
IPR037518	MPN domain	PROSITE	PS50249	MPN	coord: 354..484 score: 30.748177
IPR044098	STAMBP/STALP-like, MPN domain	CDD	cd08066	MPN_AMSH_like	coord: 352..528 e-value: 2.99501E-114 score: 333.793

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18874-RA	Carg18874-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006897	endocytosis
biological_process	GO:0090316	positive regulation of intracellular protein transport
biological_process	GO:0044090	positive regulation of vacuole organization
biological_process	GO:0071108	protein K48-linked deubiquitination
biological_process	GO:0070536	protein K63-linked deubiquitination
biological_process	GO:0016579	protein deubiquitination
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005770	late endosome
molecular_function	GO:0070122	isopeptidase activity
molecular_function	GO:0061578	Lys63-specific deubiquitinase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004843	thiol-dependent deubiquitinase