Carg18784 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18784
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmalonate--CoA ligase
LocationCarg_Chr08: 3895571 .. 3900181 (+)
RNA-Seq ExpressionCarg18784
SyntenyCarg18784
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGCTCGATTATTACAAGGTTACGAAGCAATGGAACCGGACCTACAGGTTGCTTCTGCCAATGCTGCAAGACAGTTGCGCCTTATGGTGAGTTTGTGCATCATTATTGGGGGTTTTAAAATATTATTTGACCTTTTTAACCTCTTTTAAACTGTATCCAACTTCCAAATGTCCCTACTTCTGCCTTAAAATTTACTCCCATCTTCTTCAGATGTGTGGCTCTTCTGCACTCCCTCCTCCTATCATGAAACAATGGAAGGCGGTAACTGGACACCAACTTTTGGAACGGTACGGTATGACCGAAGTAAGTGAATTTGGCTTAAGATTTTGTCAAGGTTGTATTCTAATGATATGATGCCATTTTATTTCTTTTCATCTTTTGGTAGAAAAAGCACTAAAATTACTGTTCTCTTGGTTATATTTATGTTCTAGTTTGTCATGGCAATTTCAAATCCTTTAAATGGTGAGAGAAAGATTGGAACTGTTGGCAAACCCTTGCCTGGTGTGGAGGTTAGTACTGATACTATGGAAAAGACAAATCTACAGATTTTGGTAACTTTGTTGGTTTGTCAAGATTTTTGACAGTGCAAGTAACATTCAGATTACATTCAAATCTACAGATTTTTATTTCTAGCTGTCCTAATGAATTAGACTGTTGCATTTATAGAGAGAGCTTACAAAAACTGAACAAGGGGATAAGATCTTACCCTAAATAATATATCTCAAGAAAGTTACAAAAACATTTTCTTGTTTGAATTAATAAAAAAAGATTGGCATTTACAAGAAGCCCTTGTCAAGAATTCTCCGTCGAGGGATAGGAAATGCCAAATTCGAGGAAGTTGTTCATTTTCCATTCAAAGATTTTGAGAGTAATGCATTGAGCACTTGTTTATCTTTTCACATGATCGTTAGCCATGTAAGTGGTGATGAAGACGTGCATGTAATCTCGATTGCCATATTAAAAAAGGCTAAATGTGAGATCTCACATTGGTTGGAGAGGGGTACGAAATATTCCTTTTTAGGGTGTCGAAACCTCTCCCTAGTAGATGCGTTTCAAAACCTTGAGGGGAAGCCCAAAAAGGACAATATCTGCTAGTAGTGAGCTTGGGCTGTTACAAATGATCTCAAAGTCAAAACTCTAGGCAGTGTGCGTTTGTGAGATCCCACATTGGTTGGAGAGGGAACGAAACGTTCCTTATTAGGGTGTGGAAACCTCTCCCTAGTAGACACGTTTTAAAACCTTGAGGGAAAACCCAAAAGGGAAAGCCCAAAGAAGACTATCTGCTAGCGGTGGGCTTGGACTGTTACACTAAAGGTCTTTTAGTGACACCAATCTAAAGGTCAAAATAAATATATTTATTCAATTCAAATTGTTATTTCCATGTGGTTTTTATTCATTTATAATTCTTTTGTTTTCTCCATTGTAGTCGGTTTTCACTATTCACATTCATATTGACAACAGTGTATCAAACAAATATAATCCGATCGTGTATAAATGAAGCTAGTTATCTCCGTTTCAGGACCTTTGCTTTTCACGCACATGATGGTCTCATTGTATTTTCTGTGATACAAAAAGTTACTTTTGTGTGATATAAGAAGTTTTTTTTATTTGGAATATTTTGATTACGTGGTGCAATTCTGGGCCTTGGGGCCTTGGGGTGACACGGACCTTTAGAAGAAAAATAAAAGCCAATGGTGTTGCATCTTGGTATTAATTGATTTCTCCTATCTTGGACAAAACATTGTTGCAGTCCTATAGATAATAAAAGCGTGCATAGTCATAGACATTAAATAGTATTAATGGGTATCATAAGCTTACTTTTGTTAATTTGACTCCAAAGATGTAACAAGGCAGTAAAACCAGTGTTGGTTAACCTAACCTGTGCATCTGACATAATGAGTGTCTGACCATTGAATCATATATACTAATCTGTTTCTACCTTTTAACTCTCTTTTACATGCTTTACTCTTCGTGTGTTAGGCCAAGATTGTTTCAGATGATGGGAGCAATAATGACACAAACTTGGAGGGCGAGCTATGTATTAGAAGTCCTTCAATGTTTAAAGAATATTGGAAGCTTCCCAAGGTAGGTAGAACCATACGAATACGTAATTCGAACATTTCATGTTGATTCACCTTCGTATTGGGTATCATTCTTGTATGTTTGGGATGCACTGAGCTCAACTAGTTTGAAACTTACATTCTAGCTTAAAATCTTCTCACCTGCACCTGTAACTTACAAATGGTGGGATCATATTCTAAGTACAGGTTCTTTCTGTAGGTTACCAAGGAATCATTCATTGATGGTGGATTCTTCAAAACAGGAGATGCTGCTAGACTTGATGAAGATGGTTACTACACCATACTAGGACGTAAGTTACCATATTATTTACCTCGTGCCAATACGCTACGATTATAGTTCATAGTTCATAAACTGGCTCTCCTTTTACTCTTCACTTTTATGGCCTGCTTATCAGATGACATGTTTTCTGGTCTGAGCATGTGATTAAGTAATGAATTGATACCAAGTAGACAACTAGCATTGTCTTCTACCCTGTTGAATAAAACATGGTGTCTTATTCCTTGTGGAGCATTAGAGTGTCGTGGGATCCTTTGGTGTCCCCATCTTTGCTAGTCCTGTAGGAGTACGGTGGTACATGCATCTGACAGCTAGGTGTTGTTAACAAAGCATTTCAAAGATGTTTTTGTGTGTTCATATCATGTTTGAAAACATTAGCTTGCTGAGCTTCATGCTCTTGTCAGCTAGGCTAGTTTGGTGCTATGCTCCCACACTCGCAAATTACATTAGGCAGTAGAATTGACATGTAAATTTTGTATATTTTCAGTGGCCCTGCATCTCTCTCCTATAGTGAGTTTAGCCAATAACAAGATAACTTTGAAGTACCCCACCATTAACTTATCAGTGTCCCCCTATTCTTTTTCTTGAACTCTGTAACTGTATAACTAATACCTGCCGCATCTTTCCCTCCCGCTTCTTTATTCTTTGTAATTTCATATCATGGATGAAGTTGTTTCTACCAAAAAGGAAAAGTTTAGCCATTGTCAGCCTTCTAATACCTGCATGTGAGCTGTGGGGGTCCGAGTCTCAACATCATCTATTAAAGTTCAGTCATTTCATGTGAGATCCCACATCAGTCAGAGAGGAAAACGAAACATTCTTTATAAGAGTGTGGAAACCTCTCCCCAGTAGACGCATTTTAAAAACCTTGAGGGGAAGCCTGAAACGGAAAGGGCTGTTACAAATGGCGTTAGAGCTAGACACCGGGCGATGTGCCACCGAGGAGACTGAACCCCAAAAGGGGCGGACATGAGGCAATGTGCCAGCAATGGTCTCACATTGATTGGTGTAGGGAACGAGTGCCAGCGAGGACGCTGGCCCCCCCAAAGGGAGGTGGATTGTGAGATCCCACGTCGGTTGGGAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCGTAGCCCAAAGAGCACAATATTTGTTAGCGGTGAGGCATTTCATATCAATCTTATTTCTATGTAATTTGACATTCACCTTTGTCTAACGATTCAGGTATGAGTGCCGATATCATGAAAGTCGGGGGATATAAGTTATCGGCATTAGAGATCGAATCAGTGATTTTGCAGGTAACCCCATTGCTCCTTCTATTCTCTTTTTTCTCATTCTCTTTTATCTGAAGGAAAGTGAAAGACAAACCCCCACACCCACACCCACCACAAAGAAAGGAAGAATAAAAAACATGAAATGACCACCGCCCAAATCACTTTGTGCAGCATCCGTCTGTCATAGAATGTTGTGTGTTGGGATTACCAGACAAAGACTATGGAGAACGTGTGTGTGCAATTATTGTGCTTCACCCTAACTCTAAAGCAACGCCGCCTGATCAGTCCAAGCCTAATATGAGCTTAGATGATCTCCGCACATGGGCTAAAGACAAGCTTGCTCCATACAAGGTAATGTAATCAATCCATAATGGAGTTTTTAGCTGTTAAAACTGCCATTTCAGTGTGAAAGGCAGCCTGGTTTAACAATATAACTACACCTTTTTTCCAGTTACCGACCGTGTTATTGTTGAAGGACTCGCTGCCTCGGAACGCCATGGGAAAGGTCGGTTGCAATCTACAACAGAAATTGCTCGTCTCTTTAGTTCATTCTGTCTGGTTGAGACTGAAACTTCTCTTGAACTCTGCAGGTGAACAAGAAAGAGTTGAAGAAAAAGCTGGCTGAAGAACACCAACCATTCTGTTAGTATTTCATTATTTGAGAACTTGTAATCTCTACTGTTCATAATTTATTAGCTTCGTTTATGTTATACCGAAAGATCATACGAAAAATGTTATTGAAACGGGTCGAACTCGGAGAAAATGAGGAATTTCGTGGCAGTAGGATCAATCTAAATCTCAAATGCAAGCTTAGGATTATATATCAAATATGATTATGCCCCTTTTTGGCTGTTTGATCAAACAAATATTACACTGAGACCACAAAAAGAACATATATACACACACACACACACACACACCCACACACAACTCCATATTCAAGCCTGCAATTCCTTGGGCTTCTGCAACTCTCCTTCTTC

mRNA sequence

ATGTATGCTCGATTATTACAAGGTTACGAAGCAATGGAACCGGACCTACAGGTTGCTTCTGCCAATGCTGCAAGACAGTTGCGCCTTATGATGTGTGGCTCTTCTGCACTCCCTCCTCCTATCATGAAACAATGGAAGGCGGTAACTGGACACCAACTTTTGGAACGGTACGGTATGACCGAATTTGTCATGGCAATTTCAAATCCTTTAAATGGTGAGAGAAAGATTGGAACTGTTGGCAAACCCTTGCCTGGTGTGGAGGCCAAGATTGTTTCAGATGATGGGAGCAATAATGACACAAACTTGGAGGGCGAGCTATGTATTAGAAGTCCTTCAATGTTTAAAGAATATTGGAAGCTTCCCAAGGTTACCAAGGAATCATTCATTGATGGTGGATTCTTCAAAACAGGAGATGCTGCTAGACTTGATGAAGATGGTTACTACACCATACTAGGACGTATGAGTGCCGATATCATGAAAGTCGGGGGATATAAGTTATCGGCATTAGAGATCGAATCAGTGATTTTGCAGCATCCGTCTGTCATAGAATGTTGTGTGTTGGGATTACCAGACAAAGACTATGGAGAACGTGTGTGTGCAATTATTGTGCTTCACCCTAACTCTAAAGCAACGCCGCCTGATCAGTCCAAGCCTAATATGAGCTTAGATGATCTCCGCACATGGGCTAAAGACAAGCTTGCTCCATACAAGTTACCGACCGTGTTATTGTTGAAGGACTCGCTGCCTCGGAACGCCATGGGAAAGGTGAACAAGAAAGAGTTGAAGAAAAAGCTGGCTGAAGAACACCAACCATTCTGTTAGTATTTCATTATTTGAGAACTTGTAATCTCTACTGTTCATAATTTATTAGCTTCGTTTATGTTATACCGAAAGATCATACGAAAAATGTTATTGAAACGGGTCGAACTCGGAGAAAATGAGGAATTTCGTGGCAGTAGGATCAATCTAAATCTCAAATGCAAGCTTAGGATTATATATCAAATATGATTATGCCCCTTTTTGGCTGTTTGATCAAACAAATATTACACTGAGACCACAAAAAGAACATATATACACACACACACACACACACACCCACACACAACTCCATATTCAAGCCTGCAATTCCTTGGGCTTCTGCAACTCTCCTTCTTC

Coding sequence (CDS)

ATGTATGCTCGATTATTACAAGGTTACGAAGCAATGGAACCGGACCTACAGGTTGCTTCTGCCAATGCTGCAAGACAGTTGCGCCTTATGATGTGTGGCTCTTCTGCACTCCCTCCTCCTATCATGAAACAATGGAAGGCGGTAACTGGACACCAACTTTTGGAACGGTACGGTATGACCGAATTTGTCATGGCAATTTCAAATCCTTTAAATGGTGAGAGAAAGATTGGAACTGTTGGCAAACCCTTGCCTGGTGTGGAGGCCAAGATTGTTTCAGATGATGGGAGCAATAATGACACAAACTTGGAGGGCGAGCTATGTATTAGAAGTCCTTCAATGTTTAAAGAATATTGGAAGCTTCCCAAGGTTACCAAGGAATCATTCATTGATGGTGGATTCTTCAAAACAGGAGATGCTGCTAGACTTGATGAAGATGGTTACTACACCATACTAGGACGTATGAGTGCCGATATCATGAAAGTCGGGGGATATAAGTTATCGGCATTAGAGATCGAATCAGTGATTTTGCAGCATCCGTCTGTCATAGAATGTTGTGTGTTGGGATTACCAGACAAAGACTATGGAGAACGTGTGTGTGCAATTATTGTGCTTCACCCTAACTCTAAAGCAACGCCGCCTGATCAGTCCAAGCCTAATATGAGCTTAGATGATCTCCGCACATGGGCTAAAGACAAGCTTGCTCCATACAAGTTACCGACCGTGTTATTGTTGAAGGACTCGCTGCCTCGGAACGCCATGGGAAAGGTGAACAAGAAAGAGTTGAAGAAAAAGCTGGCTGAAGAACACCAACCATTCTGTTAG

Protein sequence

MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Homology
BLAST of Carg18784 vs. NCBI nr
Match: KAG6593469.1 (Malonate--CoA ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558.1 bits (1437), Expect = 4.1e-155
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT
Sbjct: 434 MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 493

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 494 EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 553

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS
Sbjct: 554 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 613

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT
Sbjct: 614 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 673

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
           VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 674 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 706

BLAST of Carg18784 vs. NCBI nr
Match: KAG7025812.1 (Malonate--CoA ligase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558.1 bits (1437), Expect = 4.1e-155
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT
Sbjct: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS
Sbjct: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT
Sbjct: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
           VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 273

BLAST of Carg18784 vs. NCBI nr
Match: XP_022964295.1 (malonate--CoA ligase [Cucurbita moschata])

HSP 1 Score: 557.4 bits (1435), Expect = 7.0e-155
Identity = 272/273 (99.63%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT
Sbjct: 338 MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 397

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 398 EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 457

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS
Sbjct: 458 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 517

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYK+PT
Sbjct: 518 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKIPT 577

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
           VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 578 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 610

BLAST of Carg18784 vs. NCBI nr
Match: XP_023515293.1 (malonate--CoA ligase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 554.7 bits (1428), Expect = 4.5e-154
Identity = 271/273 (99.27%), Postives = 272/273 (99.63%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT
Sbjct: 338 MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 397

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 398 EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 457

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRM+ADIMKVGGYKLSALEIESVILQHPS
Sbjct: 458 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPS 517

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT
Sbjct: 518 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 577

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
            LLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 578 ELLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 610

BLAST of Carg18784 vs. NCBI nr
Match: XP_023000346.1 (malonate--CoA ligase [Cucurbita maxima])

HSP 1 Score: 548.9 bits (1413), Expect = 2.5e-152
Identity = 268/273 (98.17%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQV SA+AARQLRLMMCGSSALP PIMKQWKAVTGHQLLERYGMT
Sbjct: 338 MYARLLQGYEAMEPDLQVDSASAARQLRLMMCGSSALPTPIMKQWKAVTGHQLLERYGMT 397

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 398 EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 457

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRM+ADIMKVGGYKLSALEIESVILQHPS
Sbjct: 458 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPS 517

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYK+PT
Sbjct: 518 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKIPT 577

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
           VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 578 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 610

BLAST of Carg18784 vs. ExPASy Swiss-Prot
Match: M4IS90 (Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 6.9e-113
Identity = 197/268 (73.51%), Postives = 230/268 (85.82%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MY RL+QGYEAM+P+L+ ASA+AA+QLRLMMCGSSALP P+M+QW+ +TGH+LLERYGMT
Sbjct: 337 MYTRLIQGYEAMDPELKEASASAAKQLRLMMCGSSALPIPVMQQWQTITGHRLLERYGMT 396

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPL GERK GTVGKP PGVE +IV++D + +DT   GELC+RSPS+FKEYW+L
Sbjct: 397 EFVMAISNPLKGERKAGTVGKPFPGVEVRIVAEDENGSDTTGVGELCVRSPSLFKEYWRL 456

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           P+VTK SF D GFFKTGDA ++DEDGYY ILGR SADIMKVGGYKLSALEIESV+L+HP+
Sbjct: 457 PEVTKSSFTDDGFFKTGDAGKVDEDGYYVILGRTSADIMKVGGYKLSALEIESVLLEHPT 516

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           V ECCVLGLPDKDYGE V AIIV    +K    ++SKP +SL++L +WA+ KLAPYKLPT
Sbjct: 517 VEECCVLGLPDKDYGEAVSAIIVPAAEAKKKGEEESKPAISLEELFSWAQHKLAPYKLPT 576

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEE 269
            L L DSLPRNAMGKVNKKELKKKL  E
Sbjct: 577 RLFLWDSLPRNAMGKVNKKELKKKLTVE 604

BLAST of Carg18784 vs. ExPASy Swiss-Prot
Match: Q8H151 (Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2)

HSP 1 Score: 383.6 bits (984), Expect = 1.8e-105
Identity = 187/268 (69.78%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MY RL+QGYEAM+ ++Q +SA AAR+LRLMM GSSALP P+M QW+++TGH+LLERYGMT
Sbjct: 343 MYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYGMT 402

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMA+SNPL G R  GTVGKPLPGVEAKI  D+   ND N  GE+C++SPS+FKEYW L
Sbjct: 403 EFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDE---NDANGVGEICVKSPSLFKEYWNL 462

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           P+VTKESF + G+FKTGDA R+DEDGYY ILGR SADIMKVGGYKLSALEIES +L+HP+
Sbjct: 463 PEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPT 522

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           V ECCVLGL D DYGE V AII+    +K    D+SKP ++L++L  WAKDKLAPYKLPT
Sbjct: 523 VAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPT 582

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEE 269
            LL+ +SLPRNAMGKVNKKELKK L  +
Sbjct: 583 RLLIWESLPRNAMGKVNKKELKKSLENQ 607

BLAST of Carg18784 vs. ExPASy Swiss-Prot
Match: Q58DN7 (Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.9e-62
Identity = 128/288 (44.44%), Postives = 185/288 (64.24%), Query Frame = 0

Query: 1   MYARLLQGYEA--MEPDLQ-VASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERY 60
           +Y++L+  Y+    +P +Q    A    ++RLM+ GS+ALP P++++WK +TGH LLERY
Sbjct: 297 IYSKLMDYYDKHFTQPHVQDFVRAVCEEKIRLMVSGSAALPLPVLEKWKGITGHTLLERY 356

Query: 61  GMTEFVMAISNPLNGERKIGTVGKPLPGVEAKIVSD-------------DGSNNDTNL-- 120
           GMTE  MA+SNPL   R  G+VG PLPGVE +IVS+             +GS  +T +  
Sbjct: 357 GMTEIGMALSNPLTAARLPGSVGTPLPGVEVRIVSENPQKDSSPYLIHAEGSEENTKVTP 416

Query: 121 -----EGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSAD 180
                EGEL +R PS+F+EYW  P+ TK +F   G+FKTGD   + +DG Y I GR S D
Sbjct: 417 GFEEKEGELLVRGPSVFREYWDKPEETKAAFTSDGWFKTGDTV-VFKDGCYWIRGRTSVD 476

Query: 181 IMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSK 240
           I+K GGYK+SALE+E ++L HPS+ +  V+G+PD  +G+RV A++ L          Q  
Sbjct: 477 IIKSGGYKVSALEVERLLLAHPSITDVAVIGVPDMTWGQRVTAVVTL----------QEG 536

Query: 241 PNMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKELKKKL 266
            ++S  +L+ WA+  LAPY +P+ LLL + +PRN MGKVNK++L ++L
Sbjct: 537 HSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKRDLVRQL 573

BLAST of Carg18784 vs. ExPASy Swiss-Prot
Match: Q3URE1 (Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 2.5e-62
Identity = 130/287 (45.30%), Postives = 183/287 (63.76%), Query Frame = 0

Query: 1   MYARLLQGYEA--MEPDLQ-VASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERY 60
           +Y++LL  Y+    +P +Q    A    ++RLM+ GS+ALP P++++W++ TGH LLERY
Sbjct: 296 VYSKLLDYYDKHFTQPHVQDFVRAVCKERIRLMVSGSAALPVPLLEKWRSATGHTLLERY 355

Query: 61  GMTEFVMAISNPLNGERKIGTVGKPLPGVEAKIVSD------------DGSNNDTNL--- 120
           GMTE  MA+SNPL   R  G+VG PLPGVE +I+S+            +G+   T +   
Sbjct: 356 GMTEIGMALSNPLTEARVPGSVGTPLPGVEVRIISENPQKGSPYIIHAEGNERGTKVTPG 415

Query: 121 ----EGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADI 180
               EGEL +R PS+F+EYW  P+ TK +F   G+F+TGD A + +D  Y I GR S DI
Sbjct: 416 FEEKEGELLVRGPSVFREYWDKPEETKSAFTSDGWFRTGDTA-VFKDARYWIRGRTSVDI 475

Query: 181 MKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKP 240
           +K GGYK+SALEIE  +L HPS+ +  V+G+PD  +G+RV A++ L          Q   
Sbjct: 476 IKTGGYKVSALEIERHLLAHPSITDVAVIGVPDMTWGQRVTAVVAL----------QEGH 535

Query: 241 NMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKELKKKL 266
           ++S  DL+ WA+  LAPY +P+ LLL + +PRN MGKVNKKEL K+L
Sbjct: 536 SLSHGDLKEWARGVLAPYAVPSELLLVEEIPRNQMGKVNKKELLKQL 571

BLAST of Carg18784 vs. ExPASy Swiss-Prot
Match: Q6GLK6 (Malonate--CoA ligase ACSF3, mitochondrial OS=Xenopus laevis OX=8355 GN=acsf3 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 7.4e-59
Identity = 123/284 (43.31%), Postives = 171/284 (60.21%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQV---ASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERY 60
           +Y++L+  YE       V     A    ++RLM+ GSSALP P++++W+ +TGH LLERY
Sbjct: 299 IYSKLIAYYEQHFTHSNVREFVRAACQERIRLMVSGSSALPVPVLERWQEITGHTLLERY 358

Query: 61  GMTEFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDT----------------- 120
           GMTE  MA++NPL+G R  G VG PLPGVE + V+ +     T                 
Sbjct: 359 GMTEIGMALTNPLHGPRVPGAVGAPLPGVEVRTVTQNPRKEGTSYTTHAQGDSTGTMVSV 418

Query: 121 ---NLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSAD 180
              N EGEL +R P++FKEYW     T+E+F   G+FKTGD A + +DG Y ILGR S D
Sbjct: 419 GLENREGELQVRGPAVFKEYWNKRLDTQEAFTSDGWFKTGDTA-MYKDGTYWILGRTSVD 478

Query: 181 IMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSK 240
           I+K GGYK+SALE+E  +L HPS+ +  V+G PD  +G+RV AI+ L             
Sbjct: 479 IIKSGGYKVSALEVERHLLGHPSITDVAVIGAPDVTWGQRVAAIVKLRDGHA-------- 538

Query: 241 PNMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKEL 262
             +SL +L+ WA+  +APY +P  L+  + +PRN MGK+NKK+L
Sbjct: 539 --LSLQELKEWARAVMAPYCIPAELIRVEEIPRNQMGKINKKQL 571

BLAST of Carg18784 vs. ExPASy TrEMBL
Match: A0A6J1HKE3 (malonate--CoA ligase OS=Cucurbita moschata OX=3662 GN=LOC111464352 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.4e-155
Identity = 272/273 (99.63%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT
Sbjct: 338 MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 397

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 398 EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 457

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS
Sbjct: 458 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 517

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYK+PT
Sbjct: 518 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKIPT 577

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
           VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 578 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 610

BLAST of Carg18784 vs. ExPASy TrEMBL
Match: A0A6J1KFM3 (malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.2e-152
Identity = 268/273 (98.17%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAMEPDLQV SA+AARQLRLMMCGSSALP PIMKQWKAVTGHQLLERYGMT
Sbjct: 338 MYARLLQGYEAMEPDLQVDSASAARQLRLMMCGSSALPTPIMKQWKAVTGHQLLERYGMT 397

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL
Sbjct: 398 EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 457

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRM+ADIMKVGGYKLSALEIESVILQHPS
Sbjct: 458 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPS 517

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYK+PT
Sbjct: 518 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKIPT 577

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 274
           VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC
Sbjct: 578 VLLLKDSLPRNAMGKVNKKELKKKLAEEHQPFC 610

BLAST of Carg18784 vs. ExPASy TrEMBL
Match: A0A0A0L7U7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 239/269 (88.85%), Postives = 254/269 (94.42%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAM+ +LQ+ASA+AARQLRLMMCGSSALPPPIMKQWK VTGH+LLERYGMT
Sbjct: 341 MYARLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMT 400

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNG+RKIGTVGKP PGVEAKIV+DDGS ND++LEGELCIRSPSMFKEYWKL
Sbjct: 401 EFVMAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKL 460

Query: 121 PKVTKESFIDGGFFKTGDAARLDED-GYYTILGRMSADIMKVGGYKLSALEIESVILQHP 180
           P+VTKESFIDGGFFKTGDAARLDED GYY ILGR+SADIMKVGGYKLSALEIESVILQHP
Sbjct: 461 PEVTKESFIDGGFFKTGDAARLDEDGGYYIILGRLSADIMKVGGYKLSALEIESVILQHP 520

Query: 181 SVIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLP 240
           SVIECCVLGLPDKDYGERVCAI+VL  N K T PD S P+MSLD+LRTWAKDKLAPYKLP
Sbjct: 521 SVIECCVLGLPDKDYGERVCAIVVLQANMKTTIPDGSNPSMSLDELRTWAKDKLAPYKLP 580

Query: 241 TVLLLKDSLPRNAMGKVNKKELKKKLAEE 269
           T+LLLKDSLPRNAMGKVNKKELKKKL  E
Sbjct: 581 TMLLLKDSLPRNAMGKVNKKELKKKLIVE 609

BLAST of Carg18784 vs. ExPASy TrEMBL
Match: A0A5D3BWC7 (Malonate--CoA ligase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G00780 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.8e-133
Identity = 236/268 (88.06%), Postives = 252/268 (94.03%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAM+ +LQ+ASA+AARQLRLMMCGSSALPPPIMKQW+ VTGH+LLERYGMT
Sbjct: 239 MYARLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMT 298

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNG+RKIGTVGKP PGVEAKIV+DDGS   ++LEGELCIRSPSMFKEYWKL
Sbjct: 299 EFVMAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKL 358

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYY ILGR+SADIMKVGGYKLSALEIESVILQHPS
Sbjct: 359 PKVTKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPS 418

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           V ECCVLGLPDKDYGERVCAI+VL  N+K T PD S P+MSLD+LRTWAKDKLAPYKLPT
Sbjct: 419 VTECCVLGLPDKDYGERVCAIVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPT 478

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEE 269
           +LLLKDSLPRNAMGKVNKKELKKKL  E
Sbjct: 479 MLLLKDSLPRNAMGKVNKKELKKKLIAE 506

BLAST of Carg18784 vs. ExPASy TrEMBL
Match: A0A1S3BUN8 (malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.8e-133
Identity = 236/268 (88.06%), Postives = 252/268 (94.03%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MYARLLQGYEAM+ +LQ+ASA+AARQLRLMMCGSSALPPPIMKQW+ VTGH+LLERYGMT
Sbjct: 341 MYARLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMT 400

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMAISNPLNG+RKIGTVGKP PGVEAKIV+DDGS   ++LEGELCIRSPSMFKEYWKL
Sbjct: 401 EFVMAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKL 460

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           PKVTKESFIDGGFFKTGDAARLDEDGYY ILGR+SADIMKVGGYKLSALEIESVILQHPS
Sbjct: 461 PKVTKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPS 520

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           V ECCVLGLPDKDYGERVCAI+VL  N+K T PD S P+MSLD+LRTWAKDKLAPYKLPT
Sbjct: 521 VTECCVLGLPDKDYGERVCAIVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPT 580

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEE 269
           +LLLKDSLPRNAMGKVNKKELKKKL  E
Sbjct: 581 MLLLKDSLPRNAMGKVNKKELKKKLIAE 608

BLAST of Carg18784 vs. TAIR 10
Match: AT3G16170.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 383.6 bits (984), Expect = 1.3e-106
Identity = 187/268 (69.78%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 1   MYARLLQGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMT 60
           MY RL+QGYEAM+ ++Q +SA AAR+LRLMM GSSALP P+M QW+++TGH+LLERYGMT
Sbjct: 279 MYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYGMT 338

Query: 61  EFVMAISNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKL 120
           EFVMA+SNPL G R  GTVGKPLPGVEAKI  D+   ND N  GE+C++SPS+FKEYW L
Sbjct: 339 EFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDE---NDANGVGEICVKSPSLFKEYWNL 398

Query: 121 PKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPS 180
           P+VTKESF + G+FKTGDA R+DEDGYY ILGR SADIMKVGGYKLSALEIES +L+HP+
Sbjct: 399 PEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPT 458

Query: 181 VIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPT 240
           V ECCVLGL D DYGE V AII+    +K    D+SKP ++L++L  WAKDKLAPYKLPT
Sbjct: 459 VAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPT 518

Query: 241 VLLLKDSLPRNAMGKVNKKELKKKLAEE 269
            LL+ +SLPRNAMGKVNKKELKK L  +
Sbjct: 519 RLLIWESLPRNAMGKVNKKELKKSLENQ 543

BLAST of Carg18784 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.3e-34
Identity = 85/247 (34.41%), Postives = 142/247 (57.49%), Query Frame = 0

Query: 26  QLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFV-MAISNPL--NGERKIGTVGKP 85
           +LR +   S++L P I+ + +   G  +LE Y MTE   +  SNPL   G  K G+VGKP
Sbjct: 281 KLRFIRSCSASLAPVILSRLEEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKP 340

Query: 86  LPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAARL 145
           + G E  I+++ G   + N +GE+CIR P++ K Y   P+  K  F + G+F TGD    
Sbjct: 341 V-GQEMAILNEKGEIQEPNNKGEVCIRGPNVTKGYKNNPEANKAGF-EFGWFHTGDIGYF 400

Query: 146 DEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERV-CAI 205
           D DGY  ++GR+  +++  GG K+S +E+++V+L HP V +    G+PD+ YGE + CA+
Sbjct: 401 DTDGYLHLVGRIK-ELINRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAV 460

Query: 206 IVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKEL 265
           I   P    T        ++ +D++ + K  LA +K+P  + + D+LP+ A GK+ ++ +
Sbjct: 461 I---PREGTT--------VTEEDIKAFCKKNLAAFKVPKRVFITDNLPKTASGKIQRRIV 513

Query: 266 KKKLAEE 269
            +   E+
Sbjct: 521 AQHFLEK 513

BLAST of Carg18784 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 71/243 (29.22%), Postives = 136/243 (55.97%), Query Frame = 0

Query: 27  LRLMMCGSSALPPPIMKQ-WKAVTGHQLLERYGMTEF--VMAISNPLNGERKIGTVGKPL 86
           L+ +  G++ L   +M++  + +    L++ YGMTE   ++++ +P  G+R  G+ G   
Sbjct: 309 LKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNSGSAGMLA 368

Query: 87  PGVEAKIVS-DDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAARL 146
           PGVEA+IVS + G +   N +GE+ +R P+M K Y   P+ TKE+     +  TGD    
Sbjct: 369 PGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYF 428

Query: 147 DEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAII 206
           +EDG   ++ R+  +++K  G++++  E+E +++ HP +++  V+  PD++ GE   A +
Sbjct: 429 NEDGNLYVVDRIK-ELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFV 488

Query: 207 VLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKELK 266
           V  PNS  T            D++ +   ++APYK    +     +P++A GK+ ++EL 
Sbjct: 489 VRSPNSSITE----------QDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

BLAST of Carg18784 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 3.5e-27
Identity = 70/245 (28.57%), Postives = 133/245 (54.29%), Query Frame = 0

Query: 25  RQLRLMMCGSSALPPPIMKQWKAVTGH-QLLERYGMTEFVMAISNPLNGER--KIGTVGK 84
           + L+ +  G++ L    ++ +     H  L++ YGMTE     +   N E+  +  +VG 
Sbjct: 325 KSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGL 384

Query: 85  PLPGVEAKIVS-DDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKESFIDGGFFKTGDAA 144
             P ++AK+V    GS       GEL I+ P + K Y   PK T+ S ++  + +TGD A
Sbjct: 385 LAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIA 444

Query: 145 RLDEDGYYTILGRMSADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCA 204
             DEDGY  I+ R+  +I+K  G++++  ++E+V++ HP +I+  V   P+++ GE   A
Sbjct: 445 YFDEDGYLFIVDRIK-EIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVA 504

Query: 205 IIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTVLLLKDSLPRNAMGKVNKKE 264
            +V           + +  +S +D+ ++   ++APY+    +++ +S+P++  GK+ +KE
Sbjct: 505 FVV----------RRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKE 558

Query: 265 LKKKL 266
           LK+ L
Sbjct: 565 LKRIL 558

BLAST of Carg18784 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 116.3 bits (290), Expect = 3.8e-26
Identity = 76/268 (28.36%), Postives = 135/268 (50.37%), Query Frame = 0

Query: 9   YEAMEPDLQVASANAARQ---------LRLMMCGSSALPPPIMKQW-KAVTGHQLLERYG 68
           Y ++ P + VA  N A +         L  ++ G + L   + +++ +     ++L+ YG
Sbjct: 303 YLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYG 362

Query: 69  MTEFVMAISNPLNGE--RKIGTVGKPLPGVEAKIVS-DDGSNNDTNLEGELCIRSPSMFK 128
           +TE     ++  N E  ++ G  G   P VE KIV  D G     N  GEL IRSP++ K
Sbjct: 363 LTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMK 422

Query: 129 EYWKLPKVTKESFIDGGFFKTGDAARLDEDGYYTILGRMSADIMKVGGYKLSALEIESVI 188
            Y+K  + T  +    G+ KTGD   +D DG+  ++ R+  +++K  GY+++  E+E+++
Sbjct: 423 GYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLK-ELIKCNGYQVAPAELEALL 482

Query: 189 LQHPSVIECCVLGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAP 248
           L HP + +  V+ +PD   G+   A IV    S          N+S  ++  +   +++P
Sbjct: 483 LAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGS----------NLSESEIMGFVAKQVSP 542

Query: 249 YKLPTVLLLKDSLPRNAMGKVNKKELKK 264
           YK    +    S+P+N  GK+ ++EL K
Sbjct: 543 YKKIRKVTFLASIPKNPSGKILRRELTK 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6593469.14.1e-155100.00Malonate--CoA ligase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7025812.14.1e-155100.00Malonate--CoA ligase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022964295.17.0e-15599.63malonate--CoA ligase [Cucurbita moschata][more]
XP_023515293.14.5e-15499.27malonate--CoA ligase [Cucurbita pepo subsp. pepo][more]
XP_023000346.12.5e-15298.17malonate--CoA ligase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
M4IS906.9e-11373.51Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1[more]
Q8H1511.8e-10569.78Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2[more]
Q58DN71.9e-6244.44Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV... [more]
Q3URE12.5e-6245.30Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1... [more]
Q6GLK67.4e-5943.31Malonate--CoA ligase ACSF3, mitochondrial OS=Xenopus laevis OX=8355 GN=acsf3 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1HKE33.4e-15599.63malonate--CoA ligase OS=Cucurbita moschata OX=3662 GN=LOC111464352 PE=4 SV=1[more]
A0A6J1KFM31.2e-15298.17malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1[more]
A0A0A0L7U71.6e-13388.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1[more]
A0A5D3BWC72.8e-13388.06Malonate--CoA ligase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BUN82.8e-13388.06malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G16170.11.3e-10669.78AMP-dependent synthetase and ligase family protein [more]
AT3G48990.11.3e-3434.41AMP-dependent synthetase and ligase family protein [more]
AT4G05160.11.1e-2829.22AMP-dependent synthetase and ligase family protein [more]
AT4G19010.13.5e-2728.57AMP-dependent synthetase and ligase family protein [more]
AT1G20480.13.8e-2628.36AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 17..160
e-value: 2.0E-39
score: 135.4
NoneNo IPR availableGENE3D3.30.300.30coord: 157..272
e-value: 2.5E-28
score: 100.8
NoneNo IPR availablePANTHERPTHR43201:SF20MALONATE--COA LIGASE-LIKEcoord: 1..268
NoneNo IPR availablePANTHERPTHR43201ACYL-COA SYNTHETASEcoord: 1..268
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 20..267
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 170..255
e-value: 9.9E-17
score: 61.7
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 1..153
e-value: 1.2E-45
score: 157.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18784-RACarg18784-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0090410 malonate catabolic process
biological_process GO:0009698 phenylpropanoid metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0090409 malonyl-CoA synthetase activity
molecular_function GO:0031956 medium-chain fatty acid-CoA ligase activity