Carg18624 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18624
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionO-acyltransferase
LocationCarg_Chr12: 11867773 .. 11869321 (+)
RNA-Seq ExpressionCarg18624
SyntenyCarg18624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATCTGTGCGCGGTTGATTCGGTTGTTAATTTATTTTCGTCGTTCCCTCCGGTTTTGTCTCGGCTTATCTTTTTCGATTTTCTCTCTCATTCCGTCTATTGTTGATGTTTTCGCTTGTAGGATGGGGATCACCGATGCACTCGAGAGTACAACCGCCTTGGATAGCTCGTCAACCGCCGTGGATGCCAGCCGGTTCCTTCGTAGGAGACCGAGTGCTACAACTGGTGGAGACGAGTACGATAAAAATTTGAGGCCGAACATTCGTGGAAGTGATAGTTCGCCTCCGGATCATGCAAGTTTTAATGGTTTATTGGCTGCAAAGAGCTCTGAGGAGGTTAAGGATCGAAATGACGCTATTGAAAATCCAAATCTAATGGAACCGGAGAAGGATCCGGTGACGAGGCCGAGAGTCGAGGTAACGCTTTTGATGTTTACGCTGTGAAATTTACGTATCGGCCGACGAGCCTCTGAGCTCTGTTGCGATACTCAGAGAAGTATGTTGAAATTCTTGGTTTATTTTAGCTGTCATATTATTGAACTCGCCATTACATTCGTATATACATCCGTTTTTCCTATTAGATATCATAAGTAGTCACAAACTGGATAGCTGGAAATATTAAACGACTATCTGAGGTCGATTTTCTGTTTCTTAAGTGAAATTATGCTTTGGCCGACTGCTTATCGAAGGAATTTTTCTGTGATGGAATATTTGTTCAAACTATCTTGAAATGTCATCAAGTATACTATACACTCGTAAAACTATGGCTAACACGCCTTGTTATTTCTGGCTTAAGTTTTAGATCAGATCTGATGGTTAATGCATATATAATGCTCACCATTTTATTCACTTTAAATGACAGAGCCACGCAGATCTGTTTAACCTCTGTATAGTAGTGCTTGTTGCTGTAAACAGCAGACTATCTGTATATTTCGTTTGCTACTAAAATAAGATTTATAAGCCGTTATTAATTTATTAGCATATGTATTCAAAATTGTTAACTGACATAGGAAATGCCTTTCTTGCTCGTGCAGCCTCTCCCTGTATTCTCCCTTTCTTCTTTTCTTGTTGAGAAGATGGCCATGCAGAAACATATGTTTGAGCCTGTAAGTTTTCTTAGTTAATTCTCTGGGAAATTTTCTCAAGATGGATTCCAACTGTGTATATGATTGTTTTCTTTACTTTTTTCAGGGTAATAATTGTTCCTTCGGATGGATTTCAACTGTGTATATAATAACTAGACGCTTACTGACTGAAATTGTAATTATAACCACTGTTGCAGTTCTCCATCCAGTTTTCATGATTCTCAGGTCAGTTAAGGTACTTCGCACACAATATTTTAGGAGGATTATCAGCTGAGTGATGTTAGACAATTTCTAGCTTTATTTATAGATTGCTACTCATGTTTTTAACATGTTGAAATTGCTTACATAATGTTATTGTATATTTTTAATGTCAACATAAGCCTTATGGAGAGGTAGAATGAACCATGCTGACTTTAAGCAGACAGTTCATTATTGATATTTTATGCATAGTTCTCTTGCAATTTC

mRNA sequence

CCATCTGTGCGCGGTTGATTCGGTTGTTAATTTATTTTCGTCGTTCCCTCCGGTTTTGTCTCGGCTTATCTTTTTCGATTTTCTCTCTCATTCCGTCTATTGTTGATGTTTTCGCTTGTAGGATGGGGATCACCGATGCACTCGAGAGTACAACCGCCTTGGATAGCTCGTCAACCGCCGTGGATGCCAGCCGGTTCCTTCGTAGGAGACCGAGTGCTACAACTGGTGGAGACGAGTACGATAAAAATTTGAGGCCGAACATTCGTGGAAGTGATAGTTCGCCTCCGGATCATGCAAGTTTTAATGGTTTATTGGCTGCAAAGAGCTCTGAGGAGGTTAAGGATCGAAATGACGCTATTGAAAATCCAAATCTAATGGAACCGGAGAAGGATCCGGTGACGAGGCCGAGAGTCGAGCCTCTCCCTGTATTCTCCCTTTCTTCTTTTCTTGTTGAGAAGATGGCCATGCAGAAACATATGTTTGAGCCTGGTAATAATTGTTCCTTCGGATGGATTTCAACTGTGTATATAATAACTAGACGCTTACTGACTGAAATTGTAATTATAACCACTGTTGCAGTTCTCCATCCAGTTTTCATGATTCTCAGGTCAGTTAAGGTACTTCGCACACAATATTTTAGGAGGATTATCAGCTGAGTGATGTTAGACAATTTCTAGCTTTATTTATAGATTGCTACTCATGTTTTTAACATGTTGAAATTGCTTACATAATGTTATTGTATATTTTTAATGTCAACATAAGCCTTATGGAGAGGTAGAATGAACCATGCTGACTTTAAGCAGACAGTTCATTATTGATATTTTATGCATAGTTCTCTTGCAATTTC

Coding sequence (CDS)

ATGGGGATCACCGATGCACTCGAGAGTACAACCGCCTTGGATAGCTCGTCAACCGCCGTGGATGCCAGCCGGTTCCTTCGTAGGAGACCGAGTGCTACAACTGGTGGAGACGAGTACGATAAAAATTTGAGGCCGAACATTCGTGGAAGTGATAGTTCGCCTCCGGATCATGCAAGTTTTAATGGTTTATTGGCTGCAAAGAGCTCTGAGGAGGTTAAGGATCGAAATGACGCTATTGAAAATCCAAATCTAATGGAACCGGAGAAGGATCCGGTGACGAGGCCGAGAGTCGAGCCTCTCCCTGTATTCTCCCTTTCTTCTTTTCTTGTTGAGAAGATGGCCATGCAGAAACATATGTTTGAGCCTGGTAATAATTGTTCCTTCGGATGGATTTCAACTGTGTATATAATAACTAGACGCTTACTGACTGAAATTGTAATTATAACCACTGTTGCAGTTCTCCATCCAGTTTTCATGATTCTCAGGTCAGTTAAGGTACTTCGCACACAATATTTTAGGAGGATTATCAGCTGA

Protein sequence

MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASFNGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMFEPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKVLRTQYFRRIIS
Homology
BLAST of Carg18624 vs. NCBI nr
Match: KAG7021331.1 (hypothetical protein SDJN02_18020 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336.7 bits (862), Expect = 1.3e-88
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF
Sbjct: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF 120
           NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF
Sbjct: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF 120

Query: 121 EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKVLRTQYFRRIIS 178
           EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKVLRTQYFRRIIS
Sbjct: 121 EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKVLRTQYFRRIIS 177

BLAST of Carg18624 vs. NCBI nr
Match: KAG6586474.1 (hypothetical protein SDJN03_19207, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.8 bits (787), Expect = 6.2e-80
Identity = 165/174 (94.83%), Postives = 165/174 (94.83%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           MGITD LESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF
Sbjct: 47  MGITDVLESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 106

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVE--------PLPVFSLSSFLVEK 120
           NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVE        PLPVFSLSSFLVEK
Sbjct: 107 NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEEMPFLLVQPLPVFSLSSFLVEK 166

Query: 121 MAMQKHMFEPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKV 167
           MAMQKHMFEPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKV
Sbjct: 167 MAMQKHMFEPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILRSVKV 220

BLAST of Carg18624 vs. NCBI nr
Match: XP_022965954.1 (uncharacterized protein LOC111465677 isoform X2 [Cucurbita maxima])

HSP 1 Score: 231.1 bits (588), Expect = 7.4e-57
Identity = 130/162 (80.25%), Postives = 131/162 (80.86%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M ITDALESTTALDSS T +DASRFLRRRPSATTGGDEYDKNLR NIRGSDSSPPDHASF
Sbjct: 23  MAITDALESTTALDSSLTVLDASRFLRRRPSATTGGDEYDKNLRTNIRGSDSSPPDHASF 82

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF 120
           NGLLAAKSSEEVKDRNDAIENPNLMEPEKD VTRPRVE                      
Sbjct: 83  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDRVTRPRVE---------------------- 142

Query: 121 EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILR 163
             GNNCSFGWIS VYIITRRLLTEIVIITTVAV HPVFMILR
Sbjct: 143 --GNNCSFGWISIVYIITRRLLTEIVIITTVAVFHPVFMILR 160

BLAST of Carg18624 vs. NCBI nr
Match: XP_022965953.1 (uncharacterized protein LOC111465677 isoform X1 [Cucurbita maxima])

HSP 1 Score: 231.1 bits (588), Expect = 7.4e-57
Identity = 130/162 (80.25%), Postives = 131/162 (80.86%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M ITDALESTTALDSS T +DASRFLRRRPSATTGGDEYDKNLR NIRGSDSSPPDHASF
Sbjct: 56  MAITDALESTTALDSSLTVLDASRFLRRRPSATTGGDEYDKNLRTNIRGSDSSPPDHASF 115

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF 120
           NGLLAAKSSEEVKDRNDAIENPNLMEPEKD VTRPRVE                      
Sbjct: 116 NGLLAAKSSEEVKDRNDAIENPNLMEPEKDRVTRPRVE---------------------- 175

Query: 121 EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILR 163
             GNNCSFGWIS VYIITRRLLTEIVIITTVAV HPVFMILR
Sbjct: 176 --GNNCSFGWISIVYIITRRLLTEIVIITTVAVFHPVFMILR 193

BLAST of Carg18624 vs. NCBI nr
Match: XP_022999466.1 (diacylglycerol O-acyltransferase 1A [Cucurbita maxima] >XP_022999467.1 diacylglycerol O-acyltransferase 1A [Cucurbita maxima] >XP_022999468.1 diacylglycerol O-acyltransferase 1A [Cucurbita maxima])

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-20
Identity = 62/109 (56.88%), Postives = 74/109 (67.89%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M I D LESTT  +SS + +DA+RFLRRRP ATT G E + NLRPN+RG+DSSPPDHA  
Sbjct: 1   MAIADMLESTTPSESSLSDLDATRFLRRRPGATTAGVESETNLRPNVRGTDSSPPDHAGV 60

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFL 110
           NGL AAK  EE  DRN A++NPNL E  KDP  +        F +SS +
Sbjct: 61  NGLSAAKRCEEDIDRNCAVQNPNLTETAKDPGDKAESRGNAKFDVSSVI 109

BLAST of Carg18624 vs. ExPASy TrEMBL
Match: A0A6J1HSC3 (uncharacterized protein LOC111465677 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465677 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.6e-57
Identity = 130/162 (80.25%), Postives = 131/162 (80.86%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M ITDALESTTALDSS T +DASRFLRRRPSATTGGDEYDKNLR NIRGSDSSPPDHASF
Sbjct: 23  MAITDALESTTALDSSLTVLDASRFLRRRPSATTGGDEYDKNLRTNIRGSDSSPPDHASF 82

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF 120
           NGLLAAKSSEEVKDRNDAIENPNLMEPEKD VTRPRVE                      
Sbjct: 83  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDRVTRPRVE---------------------- 142

Query: 121 EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILR 163
             GNNCSFGWIS VYIITRRLLTEIVIITTVAV HPVFMILR
Sbjct: 143 --GNNCSFGWISIVYIITRRLLTEIVIITTVAVFHPVFMILR 160

BLAST of Carg18624 vs. ExPASy TrEMBL
Match: A0A6J1HQG6 (uncharacterized protein LOC111465677 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465677 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.6e-57
Identity = 130/162 (80.25%), Postives = 131/162 (80.86%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M ITDALESTTALDSS T +DASRFLRRRPSATTGGDEYDKNLR NIRGSDSSPPDHASF
Sbjct: 56  MAITDALESTTALDSSLTVLDASRFLRRRPSATTGGDEYDKNLRTNIRGSDSSPPDHASF 115

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFLVEKMAMQKHMF 120
           NGLLAAKSSEEVKDRNDAIENPNLMEPEKD VTRPRVE                      
Sbjct: 116 NGLLAAKSSEEVKDRNDAIENPNLMEPEKDRVTRPRVE---------------------- 175

Query: 121 EPGNNCSFGWISTVYIITRRLLTEIVIITTVAVLHPVFMILR 163
             GNNCSFGWIS VYIITRRLLTEIVIITTVAV HPVFMILR
Sbjct: 176 --GNNCSFGWISIVYIITRRLLTEIVIITTVAVFHPVFMILR 193

BLAST of Carg18624 vs. ExPASy TrEMBL
Match: A0A6J1KJS9 (O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111493820 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 5.4e-21
Identity = 62/109 (56.88%), Postives = 74/109 (67.89%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M I D LESTT  +SS + +DA+RFLRRRP ATT G E + NLRPN+RG+DSSPPDHA  
Sbjct: 1   MAIADMLESTTPSESSLSDLDATRFLRRRPGATTAGVESETNLRPNVRGTDSSPPDHAGV 60

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFL 110
           NGL AAK  EE  DRN A++NPNL E  KDP  +        F +SS +
Sbjct: 61  NGLSAAKRCEEDIDRNCAVQNPNLTETAKDPGDKAESRGNAKFDVSSVI 109

BLAST of Carg18624 vs. ExPASy TrEMBL
Match: A0A6J1G356 (O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111450373 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 9.2e-21
Identity = 63/109 (57.80%), Postives = 73/109 (66.97%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRPSATTGGDEYDKNLRPNIRGSDSSPPDHASF 60
           M I D LESTT  +SS + +DASRFLRRRP ATT G E + NLRPN+RG+DSSPPDHA  
Sbjct: 1   MAIADMLESTTPSESSLSDLDASRFLRRRPGATTAGVESETNLRPNVRGTDSSPPDHAGA 60

Query: 61  NGLLAAKSSEEVKDRNDAIENPNLMEPEKDPVTRPRVEPLPVFSLSSFL 110
           NGL AAK  EE  DRN A +NPNL E  KDP  +        F +SS +
Sbjct: 61  NGLSAAKRCEEDIDRNYAAQNPNLTETAKDPGDKAESRGNAKFDVSSVI 109

BLAST of Carg18624 vs. ExPASy TrEMBL
Match: A0A5D3CKN1 (O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001040 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-18
Identity = 94/248 (37.90%), Postives = 109/248 (43.95%), Query Frame = 0

Query: 1   MGITDALESTTALDSSSTAVDASRFLRRRP-SATTGGDEYDKNLRPNIRGSDSSPPDHAS 60
           M I D LESTT  DSS + +DA+RFLRRRP SATT G E D   RP+IRG+ +SPPDHA+
Sbjct: 44  MAIADTLESTTPSDSSLSDLDATRFLRRRPTSATTAGTESDTIFRPDIRGTHTSPPDHAA 103

Query: 61  FNGLLAAKSSEEVKDRNDAIENPNLMEPEK------------------------------ 120
            NGL  AK  +E  DRNDA+ENPNL+E EK                              
Sbjct: 104 VNGLSPAKRFQEDNDRNDAVENPNLIETEKVSGEMAESRGEANSDVSAVKFAYRPSAPAH 163

Query: 121 ----------DPVTR-------------------------------------PRV----- 163
                     D + R                                     P V     
Sbjct: 164 RRVKESPLSSDAIFRQSHAGLFNLCIVVLVAVNSRLIIENLMKFKILARLATPNVLAMPR 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021331.11.3e-88100.00hypothetical protein SDJN02_18020 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6586474.16.2e-8094.83hypothetical protein SDJN03_19207, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022965954.17.4e-5780.25uncharacterized protein LOC111465677 isoform X2 [Cucurbita maxima][more]
XP_022965953.17.4e-5780.25uncharacterized protein LOC111465677 isoform X1 [Cucurbita maxima][more]
XP_022999466.11.1e-2056.88diacylglycerol O-acyltransferase 1A [Cucurbita maxima] >XP_022999467.1 diacylgly... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1HSC33.6e-5780.25uncharacterized protein LOC111465677 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HQG63.6e-5780.25uncharacterized protein LOC111465677 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KJS95.4e-2156.88O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111493820 PE=3 SV=1[more]
A0A6J1G3569.2e-2157.80O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111450373 PE=3 SV=1[more]
A0A5D3CKN11.1e-1837.90O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..95

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18624-RACarg18624-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016746 acyltransferase activity