Carg16955 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16955
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Lipoyl(octanoyl) transferase
Location	Carg_Chr10: 5095312 .. 5095959 (+)
RNA-Seq Expression	Carg16955
Synteny	Carg16955

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATTGTCAGCTACAGAGAAGCCCTCCATTTGCAAGAAAAACTTGTGTCTGCTAGAAAAGCTAGTGAAATCCCAGACACCCTCTTATCCCTGCAACATCCACCCACATACACACTTGGAAAAAGGCGCACGGATCACAATCTGTTAATCCCCGAGTCGGATCTAGAGAAGCTAGGAGCTGAACTTCACTACACTGAGAGAGGGGGAGACATTACATTTCATGGTCCAAATCAGGCCATTTTGTACCCAATAATTTCCCTTAGAGATATTGGAATTGGGGCTAGAAAGTATGTGGAGAAACTGGAACAGACCATGATTGAGATGGCTGGTCTGTATGGAGTGAGAGCTAGAGCTGGGGAGAAGGGGGAAACGGGAGTTTGGGTTGGAAATAGGAAGATTGGAGCCATTGGGGTTAGGATTTCAAGTGGGATTACTTCTCATGGTCTTGCTTTCAATGTGGATCCTGATCTGAGTTATTTTAAGCATATTGTGCCTTGTGGGATTGCTGATAAAGATGTGACATCTTTGAGAAGGGAGACTGGGGAAGTTCTTCCCAGTGATGAGGTTGTGGAAGAACAGCTAATTTCTTGTTTTGTTAAGTTATTTGGTTATAGTAGATCAGTCGAGGAGAATTCTTGTATGTAA

mRNA sequence

ATGGGGATTGTCAGCTACAGAGAAGCCCTCCATTTGCAAGAAAAACTTGTGTCTGCTAGAAAAGCTAGTGAAATCCCAGACACCCTCTTATCCCTGCAACATCCACCCACATACACACTTGGAAAAAGGCGCACGGATCACAATCTGTTAATCCCCGAGTCGGATCTAGAGAAGCTAGGAGCTGAACTTCACTACACTGAGAGAGGGGGAGACATTACATTTCATGGTCCAAATCAGGCCATTTTGTACCCAATAATTTCCCTTAGAGATATTGGAATTGGGGCTAGAAAGTATGTGGAGAAACTGGAACAGACCATGATTGAGATGGCTGGTCTGTATGGAGTGAGAGCTAGAGCTGGGGAGAAGGGGGAAACGGGAGTTTGGGTTGGAAATAGGAAGATTGGAGCCATTGGGGTTAGGATTTCAAGTGGGATTACTTCTCATGGTCTTGCTTTCAATGTGGATCCTGATCTGAGTTATTTTAAGCATATTGTGCCTTGTGGGATTGCTGATAAAGATGTGACATCTTTGAGAAGGGAGACTGGGGAAGTTCTTCCCAGTGATGAGGTTGTGGAAGAACAGCTAATTTCTTGTTTTGTTAAGTTATTTGGTTATAGTAGATCAGTCGAGGAGAATTCTTGTATGTAA

Coding sequence (CDS)

ATGGGGATTGTCAGCTACAGAGAAGCCCTCCATTTGCAAGAAAAACTTGTGTCTGCTAGAAAAGCTAGTGAAATCCCAGACACCCTCTTATCCCTGCAACATCCACCCACATACACACTTGGAAAAAGGCGCACGGATCACAATCTGTTAATCCCCGAGTCGGATCTAGAGAAGCTAGGAGCTGAACTTCACTACACTGAGAGAGGGGGAGACATTACATTTCATGGTCCAAATCAGGCCATTTTGTACCCAATAATTTCCCTTAGAGATATTGGAATTGGGGCTAGAAAGTATGTGGAGAAACTGGAACAGACCATGATTGAGATGGCTGGTCTGTATGGAGTGAGAGCTAGAGCTGGGGAGAAGGGGGAAACGGGAGTTTGGGTTGGAAATAGGAAGATTGGAGCCATTGGGGTTAGGATTTCAAGTGGGATTACTTCTCATGGTCTTGCTTTCAATGTGGATCCTGATCTGAGTTATTTTAAGCATATTGTGCCTTGTGGGATTGCTGATAAAGATGTGACATCTTTGAGAAGGGAGACTGGGGAAGTTCTTCCCAGTGATGAGGTTGTGGAAGAACAGCTAATTTCTTGTTTTGTTAAGTTATTTGGTTATAGTAGATCAGTCGAGGAGAATTCTTGTATGTAA

Protein sequence

MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAGEKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRETGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM

Homology

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BLAST of Carg16955 vs. NCBI nr
Match: XP_022960707.1 (octanoyltransferase [Cucurbita moschata] >KAG6590228.1 Octanoyltransferase LIP2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 435.3 bits (1118), Expect = 3.1e-118
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 12  MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG
Sbjct: 72  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE
Sbjct: 132 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 216
           TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM
Sbjct: 192 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 226

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BLAST of Carg16955 vs. NCBI nr
Match: KAG7023868.1 (Octanoyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 435.3 bits (1118), Expect = 3.1e-118
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG
Sbjct: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE
Sbjct: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 216
           TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM
Sbjct: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 215

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BLAST of Carg16955 vs. NCBI nr
Match: XP_022988203.1 (octanoyltransferase [Cucurbita maxima])

HSP 1 Score: 424.5 bits (1090), Expect = 5.5e-115
Identity = 210/215 (97.67%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIV YREALHLQEKLVSARKASEIPDTLLSLQHP TYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 12  MGIVCYREALHLQEKLVSARKASEIPDTLLSLQHPHTYTLGKRRTDHNLLIPESDLEKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGV+ARAG
Sbjct: 72  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 216
           TGEVLPS EVVEEQLISCFVKLFGYSRSVEENSCM
Sbjct: 192 TGEVLPSQEVVEEQLISCFVKLFGYSRSVEENSCM 226

[Close]

BLAST of Carg16955 vs. NCBI nr
Match: XP_023516852.1 (octanoyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 420.2 bits (1079), Expect = 1.0e-113
Identity = 209/215 (97.21%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 12  MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTM+EMAGLYGVRARAG
Sbjct: 72  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMMEMAGLYGVRARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 216
           T EVLPS EVVEEQLISCFVKLFGY RSVEENS M
Sbjct: 192 TVEVLPSQEVVEEQLISCFVKLFGYGRSVEENSHM 226

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BLAST of Carg16955 vs. NCBI nr
Match: XP_038879976.1 (octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octanoyltransferase LIP2, mitochondrial [Benincasa hispida])

HSP 1 Score: 388.7 bits (997), Expect = 3.4e-104
Identity = 190/213 (89.20%), Postives = 206/213 (96.71%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIV+Y++ALHLQEKLVSARKAS+IPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 12  MGIVNYKDALHLQEKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQ+ILYPIISLRD+G GARKYVEKLE TMIEMA LYGV+ARAG
Sbjct: 72  AELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLELTMIEMACLYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGIADKDVTSLR+E
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISNGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRKE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRS-VEEN 213
           + EVLPS+EV+EEQLISCFVKLFGY ++ VE+N
Sbjct: 192 SVEVLPSEEVMEEQLISCFVKLFGYCKTQVEKN 224

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BLAST of Carg16955 vs. ExPASy Swiss-Prot
Match: Q9SXP7 (Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 6.9e-92
Identity = 160/205 (78.05%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           +G V+Y ++L LQEKLVS RKA +IPDTLLSLQHPPTYTLGKRRTDHNLLIPES+L K+G
Sbjct: 12  LGTVNYLKSLKLQEKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYT+RGGDITFHGP+QAILYPIISLR IG GAR YVE LE++MIE A +YGV+ARAG
Sbjct: 72  AELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
            K ETGVWVG+RKIGAIGVRISSGITSHGLA N+DPD+ YF+HIVPCGIADK+VTSLRRE
Sbjct: 132 NKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGY 206
           T  +LPS+EV+ EQL+SC  K F Y
Sbjct: 192 TDTLLPSEEVIHEQLVSCLAKAFSY 216

[Close]

BLAST of Carg16955 vs. ExPASy Swiss-Prot
Match: A4J246 (Octanoyltransferase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=lipB PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.1e-44
Identity = 88/210 (41.90%), Postives = 134/210 (63.81%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           +G + Y++AL +QEKL+  R+ +++ D +L LQHPPT TLG R   +N+L+PE +L++ G
Sbjct: 8   LGEIDYQDALTMQEKLLLLRQQNKVEDIMLLLQHPPTLTLGTRENRYNILVPEVELKRQG 67

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
             +  + RGGD+T+HGP Q + YPI+ L   G   R+YV K+E+T I++         + 
Sbjct: 68  VNIFKSNRGGDVTYHGPGQIVGYPIVDLNGHGKSIREYVHKIEETFIQLLKEEYDLTASR 127

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           E    GVW+GN KI AIG  +   +T HG AFNV+ +LS+F  I PCGI D+ VTSL++ 
Sbjct: 128 ESKYHGVWLGNEKITAIGCAVKRWVTMHGFAFNVNTNLSHFNLINPCGITDRGVTSLQKI 187

Query: 181 TGEVLPSD-EVVEEQLISCFVKLFGYSRSV 210
            G+  P D E V +Q+I+ F ++F +   +
Sbjct: 188 FGQ--PQDMEKVYKQVITYFSRVFDFEPEI 215

[Close]

BLAST of Carg16955 vs. ExPASy Swiss-Prot
Match: A4XHV1 (Octanoyltransferase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) OX=351627 GN=lipB PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.2e-43
Identity = 90/203 (44.33%), Postives = 127/203 (62.56%), Query Frame = 0


Query: 3   IVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAE 62
           +V Y +ALHLQE+L   R A+E+ DTLL LQHPP  T+G+R    N+LI +  L ++G +
Sbjct: 10  MVEYEDALHLQERLHKLRVANELEDTLLLLQHPPVITIGRRGKWENILISKEKLLQMGVK 69

Query: 63  LHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIE-MAGLYGVRARAGE 122
           +    RGGD+T+HGP Q + YPI  L  +G   +++V  LE+  I  +   YG+ A   E
Sbjct: 70  VFEVTRGGDVTYHGPGQIVGYPIFDLGTVGKDIKRFVWLLEEVFINLLKDEYGIEAYRDE 129

Query: 123 KGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRET 182
           K  TGVWVG  KI AIG+ +   IT HG AFNV+ +L +F  I+PCG+ D+ VTSL +  
Sbjct: 130 KQYTGVWVGGEKIVAIGIAVKKWITMHGFAFNVNTNLEHFSWIIPCGLKDRGVTSLEKLL 189

Query: 183 GEVLPSDEVVEEQLISCFVKLFG 205
           G  +  D+VV  ++   F K+FG
Sbjct: 190 GHKVEFDDVV-YKVAKYFGKVFG 211

[Close]

BLAST of Carg16955 vs. ExPASy Swiss-Prot
Match: Q1AT14 (Octanoyltransferase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=lipB PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.9e-42
Identity = 99/207 (47.83%), Postives = 127/207 (61.35%), Query Frame = 0


Query: 2   GIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTD-HNLLIPESDLEKLG 61
           G+  Y EA  LQ +LV  R++ EIPDTLL L+HPP YT+G+   D  NL   E  L  LG
Sbjct: 17  GLTPYAEAWELQRELVRKRRSGEIPDTLLLLEHPPVYTVGRAARDASNLGAGEEYLRSLG 76

Query: 62  AELHYTERGGDITFHGPNQAILYPIISL--RDIGIGARKYVEKLEQTMIEMAGLYGVRAR 121
           AE+ +++RGGD TFHGP Q + YPII L  RD      +Y+  LE+ +I     YG+  R
Sbjct: 77  AEVFWSDRGGDATFHGPGQLVGYPIIRLKVRD----THRYLRDLEEVVIRALAGYGLEGR 136

Query: 122 AGEKGETGVWVGNRKIGAIGVRISSG-ITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSL 181
              +  TGVWV   KI AIGV+ SSG I SHG A NV+ DLS+F  I PCGI +  VTSL
Sbjct: 137 RHPR-YTGVWVNGSKICAIGVKFSSGWIASHGFALNVNTDLSWFDRITPCGIREYGVTSL 196

Query: 182 RRETGEVLPSDEVVEEQLISCFVKLFG 205
            RE G  +P    VE +++S F ++ G
Sbjct: 197 ERELGREIPL-AGVEREIVSGFREVLG 217

[Close]

BLAST of Carg16955 vs. ExPASy Swiss-Prot
Match: B9MQ23 (Octanoyltransferase OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) OX=521460 GN=lipB PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.9e-41
Identity = 86/210 (40.95%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           +G V Y+EAL +QE++ + R   +I DTLL L+HPP  T+G+R +  N+L+ +  L+K+ 
Sbjct: 8   LGEVEYQEALFIQERIWALRVEKKIGDTLLLLEHPPVITIGRRGSKKNILVSDEFLKKMD 67

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIE-MAGLYGVRARA 120
            ++    RGGD+T+HGP Q + YPI  L       +K+V  LE+  I  +   +G  A  
Sbjct: 68  VKVFEVSRGGDVTYHGPGQLVGYPIFDLALTDRDIKKFVYLLEEVFIRLLKDQFGKEAHR 127

Query: 121 GEKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRR 180
            E   TGVWVGN KI AIG+ +   +T HG AFNV+ +L +F  IVPCG+ D+ VTSL R
Sbjct: 128 DENKYTGVWVGNDKIVAIGIAVKKWVTMHGFAFNVNTNLEHFSWIVPCGLKDRGVTSLER 187

Query: 181 ETGEVLPSDEVVEEQLISCFVKLFGYSRSV 210
             G  +  ++VV +++ + F K+FG S ++
Sbjct: 188 LIGSTIRFEDVV-DKVQTYFGKVFGKSLNI 216

[Close]

BLAST of Carg16955 vs. ExPASy TrEMBL
Match: A0A6J1H866 (Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111461425 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.5e-118
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 12  MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG
Sbjct: 72  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE
Sbjct: 132 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 216
           TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM
Sbjct: 192 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 226

[Close]

BLAST of Carg16955 vs. ExPASy TrEMBL
Match: A0A6J1JLK8 (Lipoyl(octanoyl) transferase OS=Cucurbita maxima OX=3661 GN=LOC111485511 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.7e-115
Identity = 210/215 (97.67%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIV YREALHLQEKLVSARKASEIPDTLLSLQHP TYTLGKRRTDHNLLIPESDLEKLG
Sbjct: 12  MGIVCYREALHLQEKLVSARKASEIPDTLLSLQHPHTYTLGKRRTDHNLLIPESDLEKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGV+ARAG
Sbjct: 72  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEENSCM 216
           TGEVLPS EVVEEQLISCFVKLFGYSRSVEENSCM
Sbjct: 192 TGEVLPSQEVVEEQLISCFVKLFGYSRSVEENSCM 226

[Close]

BLAST of Carg16955 vs. ExPASy TrEMBL
Match: A0A5A7TLG4 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00760 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.2e-104
Identity = 187/211 (88.63%), Postives = 201/211 (95.26%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIV+Y+EALHLQEKLVSARK+S+IPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLG
Sbjct: 12  MGIVNYKEALHLQEKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQ+ILYPIISLRD+G GARKYVEKLEQTMIEMA LYGV+ARAG
Sbjct: 72  AELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+E
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEE 212
           T EVLP +EV+EEQLISCFVKLFGY ++  E
Sbjct: 192 TKEVLPGEEVLEEQLISCFVKLFGYCKTQVE 222

[Close]

BLAST of Carg16955 vs. ExPASy TrEMBL
Match: A0A5D3DTA6 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold699G00090 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.2e-104
Identity = 187/211 (88.63%), Postives = 201/211 (95.26%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIV+Y+EALHLQEKLVSARK+S+IPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLG
Sbjct: 12  MGIVNYKEALHLQEKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQ+ILYPIISLRD+G GARKYVEKLEQTMIEMA LYGV+ARAG
Sbjct: 72  AELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+E
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEE 212
           T EVLP +EV+EEQLISCFVKLFGY ++  E
Sbjct: 192 TKEVLPGEEVLEEQLISCFVKLFGYCKTQME 222

[Close]

BLAST of Carg16955 vs. ExPASy TrEMBL
Match: A0A1S3BQW0 (Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103492510 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 6.2e-104
Identity = 187/211 (88.63%), Postives = 201/211 (95.26%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           MGIV+Y+EALHLQEKLVSARK+S+IPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLG
Sbjct: 12  MGIVNYKEALHLQEKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYTERGGDITFHGPNQ+ILYPIISLRD+G GARKYVEKLEQTMIEMA LYGV+ARAG
Sbjct: 72  AELHYTERGGDITFHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
           EKGETGVWVG+RKIGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+E
Sbjct: 132 EKGETGVWVGDRKIGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGYSRSVEE 212
           T EVLP +EV+EEQLISCFVKLFGY ++  E
Sbjct: 192 TKEVLPGEEVLEEQLISCFVKLFGYCKTQVE 222

[Close]

BLAST of Carg16955 vs. TAIR 10
Match: AT1G04640.1 (lipoyltransferase 2 )

HSP 1 Score: 338.2 bits (866), Expect = 4.9e-93
Identity = 160/205 (78.05%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           +G V+Y ++L LQEKLVS RKA +IPDTLLSLQHPPTYTLGKRRTDHNLLIPES+L K+G
Sbjct: 12  LGTVNYLKSLKLQEKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYT+RGGDITFHGP+QAILYPIISLR IG GAR YVE LE++MIE A +YGV+ARAG
Sbjct: 72  AELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
            K ETGVWVG+RKIGAIGVRISSGITSHGLA N+DPD+ YF+HIVPCGIADK+VTSLRRE
Sbjct: 132 NKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGY 206
           T  +LPS+EV+ EQL+SC  K F Y
Sbjct: 192 TDTLLPSEEVIHEQLVSCLAKAFSY 216

[Close]

BLAST of Carg16955 vs. TAIR 10
Match: AT1G04640.2 (lipoyltransferase 2 )

HSP 1 Score: 338.2 bits (866), Expect = 4.9e-93
Identity = 160/205 (78.05%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MGIVSYREALHLQEKLVSARKASEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLG 60
           +G V+Y ++L LQEKLVS RKA +IPDTLLSLQHPPTYTLGKRRTDHNLLIPES+L K+G
Sbjct: 12  LGTVNYLKSLKLQEKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIG 71

Query: 61  AELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIEMAGLYGVRARAG 120
           AELHYT+RGGDITFHGP+QAILYPIISLR IG GAR YVE LE++MIE A +YGV+ARAG
Sbjct: 72  AELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAG 131

Query: 121 EKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTSLRRE 180
            K ETGVWVG+RKIGAIGVRISSGITSHGLA N+DPD+ YF+HIVPCGIADK+VTSLRRE
Sbjct: 132 NKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRE 191

Query: 181 TGEVLPSDEVVEEQLISCFVKLFGY 206
           T  +LPS+EV+ EQL+SC  K F Y
Sbjct: 192 TDTLLPSEEVIHEQLVSCLAKAFSY 216

[Close]

BLAST of Carg16955 vs. TAIR 10
Match: AT4G31050.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 141.7 bits (356), Expect = 6.7e-34
Identity = 81/214 (37.85%), Postives = 126/214 (58.88%), Query Frame = 0


Query: 3   IVSYREALHLQEKLVSARKA-----SEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLE 62
           +V Y++A   Q+ +V  +K       +  DT++ LQH P YT+G   T+  L     D++
Sbjct: 54  LVPYKKAWSWQKSIVEEKKTLIDRNQDCADTVILLQHSPVYTMGTASTEDYL---NFDIK 113

Query: 63  KLGAELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIE-MAGLYGVR 122
                ++ TERGG++T+HGP Q ++YPII+LR+  +    Y+  LE+ +I  ++  + ++
Sbjct: 114 DAPFNVYRTERGGEVTYHGPGQLVMYPIINLRNHEMDLHWYLRMLEEIVIRVLSSTFSIK 173

Query: 123 ARAGEKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTS 182
           A   + G TGVWVGN+K+ AIG+R+S  IT HGLA NV  DL+ F  IVPCGI D+ V +
Sbjct: 174 ASRLD-GLTGVWVGNQKVAAIGIRVSKWITYHGLALNVTTDLTPFNWIVPCGIRDRKVGN 233

Query: 183 LR--RETGEVLPSD-----EVVEEQLISCFVKLF 204
           ++   E GE    D     ++V E L+  F + F
Sbjct: 234 IKGLLEDGEHGMVDDLRLIDIVHESLLKEFSEAF 263

[Close]

BLAST of Carg16955 vs. TAIR 10
Match: AT1G47578.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 141.0 bits (354), Expect = 1.1e-33
Identity = 79/215 (36.74%), Postives = 130/215 (60.47%), Query Frame = 0


Query: 3   IVSYREALHLQEKLVSARKA-----SEIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLE 62
           ++ Y++A   Q+ +++ +KA      E  D+L+ LQHP  YT+G   +++ L     D++
Sbjct: 56  LIPYKKAWSWQKSILNEKKALIDKNQECSDSLIILQHPSVYTMGTGSSENYL---NFDIK 115

Query: 63  KLGAELHYTERGGDITFHGPNQAILYPIISLRDIGIGARKYVEKLEQTMIE-MAGLYGVR 122
               +++ TERGG++T+HGP Q ++YPII+LR+  +    Y+ KLE+ +I  ++  + + 
Sbjct: 116 NAPFDVYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRKLEEVVIRVLSSAFAIN 175

Query: 123 ARAGEKGETGVWVGNRKIGAIGVRISSGITSHGLAFNVDPDLSYFKHIVPCGIADKDVTS 182
           A   + G TGVWVGN+K+ AIG+R+S  +T HGLA NV  DL+ F  IVPCGI ++ V S
Sbjct: 176 ASRLD-GFTGVWVGNKKMAAIGIRVSKWMTYHGLALNVTTDLTPFNSIVPCGIRNRGVGS 235

Query: 183 LRR--ETG------EVLPSDEVVEEQLISCFVKLF 204
           ++   E G      E L   ++  E L+  F ++F
Sbjct: 236 VKGLIEDGEHYNKLEDLQLLDIAHESLLKEFSEVF 266

The following BLAST results are available for this feature:

BLAST of Carg16955 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960707.1	3.1e-118	100.00	octanoyltransferase [Cucurbita moschata] >KAG6590228.1 Octanoyltransferase LIP2,...	[more]
KAG7023868.1	3.1e-118	100.00	Octanoyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022988203.1	5.5e-115	97.67	octanoyltransferase [Cucurbita maxima]	[more]
XP_023516852.1	1.0e-113	97.21	octanoyltransferase [Cucurbita pepo subsp. pepo]	[more]
XP_038879976.1	3.4e-104	89.20	octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octa...	[more]

BLAST of Carg16955 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXP7	6.9e-92	78.05	Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 ...	[more]
A4J246	4.1e-44	41.90	Octanoyltransferase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=lipB...	[more]
A4XHV1	1.2e-43	44.33	Octanoyltransferase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 /...	[more]
Q1AT14	2.9e-42	47.83	Octanoyltransferase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / ...	[more]
B9MQ23	1.9e-41	40.95	Octanoyltransferase OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6...	[more]

BLAST of Carg16955 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H866	1.5e-118	100.00	Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111461425 PE=3 ...	[more]
A0A6J1JLK8	2.7e-115	97.67	Lipoyl(octanoyl) transferase OS=Cucurbita maxima OX=3661 GN=LOC111485511 PE=3 SV...	[more]
A0A5A7TLG4	6.2e-104	88.63	Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A5D3DTA6	6.2e-104	88.63	Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BQW0	6.2e-104	88.63	Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103492510 PE=3 SV=1	[more]

BLAST of Carg16955 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04640.1	4.9e-93	78.05	lipoyltransferase 2	[more]
AT1G04640.2	4.9e-93	78.05	lipoyltransferase 2	[more]
AT4G31050.1	6.7e-34	37.85	Biotin/lipoate A/B protein ligase family	[more]
AT1G47578.1	1.1e-33	36.74	Biotin/lipoate A/B protein ligase family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 1..204 e-value: 7.2E-74 score: 249.6
None	No IPR available	PANTHER	PTHR10993	OCTANOYLTRANSFERASE	coord: 3..208
None	No IPR available	PANTHER	PTHR10993:SF7	LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED	coord: 3..208
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 2..205
IPR000544	Octanoyltransferase	PIRSF	PIRSF016262	LPLase	coord: 1..213 e-value: 2.3E-65 score: 217.9
IPR000544	Octanoyltransferase	TIGRFAM	TIGR00214	TIGR00214	coord: 21..204 e-value: 6.5E-45 score: 150.9
IPR000544	Octanoyltransferase	HAMAP	MF_00013	LipB	coord: 1..206 score: 30.611549
IPR000544	Octanoyltransferase	CDD	cd16444	LipB	coord: 1..203 e-value: 8.41523E-97 score: 278.22
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PFAM	PF03099	BPL_LplA_LipB	coord: 48..157 e-value: 2.7E-9 score: 37.0
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PROSITE	PS51733	BPL_LPL_CATALYTIC	coord: 23..207 score: 61.206017

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16955-RA	Carg16955-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009249	protein lipoylation
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0033819	lipoyl(octanoyl) transferase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0102555	octanoyl transferase activity (acting on glycine-cleavage complex H protein)