Carg15752 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15752
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF-hand family protein
Location	Carg_Chr04: 18671949 .. 18672305 (+)
RNA-Seq Expression	Carg15752
Synteny	Carg15752

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGACTTGCACTCATTTCTAGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTATGAATCGATATCGAAGCAAAACAAAGGCGAGTGCAAGGGAGGTGTGAGCGATGTCAACGCAAGAGAGGAAGATGTCGACATTGTATATATGGCGTTTAAAGTGTTCGATATGAACGATGATGGCTTCATTTCTTGTGAGGAGCTACAACTTGTGTTGTCAAGACTAGAGCTTTGGGATGTAAACACAACTGATACTGATTGTAAAAGTATGATCCGCGCTTATGATACCAATTTGGATGGGATGCTTGATTTCGAGGAGTTTAAGAACATGATGTCGTTGACAAGTTCTTGA

mRNA sequence

ATGGAGGACTTGCACTCATTTCTAGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTATGAATCGATATCGAAGCAAAACAAAGGCGAGTGCAAGGGAGGTGTGAGCGATGTCAACGCAAGAGAGGAAGATGTCGACATTGTATATATGGCGTTTAAAGTGTTCGATATGAACGATGATGGCTTCATTTCTTGTGAGGAGCTACAACTTGTGTTGTCAAGACTAGAGCTTTGGGATGTAAACACAACTGATACTGATTGTAAAAGTATGATCCGCGCTTATGATACCAATTTGGATGGGATGCTTGATTTCGAGGAGTTTAAGAACATGATGTCGTTGACAAGTTCTTGA

Coding sequence (CDS)

ATGGAGGACTTGCACTCATTTCTAGAGAAACCAAGCCTCGACTTCAACGAGTTCTTGTTCTTTTATGAATCGATATCGAAGCAAAACAAAGGCGAGTGCAAGGGAGGTGTGAGCGATGTCAACGCAAGAGAGGAAGATGTCGACATTGTATATATGGCGTTTAAAGTGTTCGATATGAACGATGATGGCTTCATTTCTTGTGAGGAGCTACAACTTGTGTTGTCAAGACTAGAGCTTTGGGATGTAAACACAACTGATACTGATTGTAAAAGTATGATCCGCGCTTATGATACCAATTTGGATGGGATGCTTGATTTCGAGGAGTTTAAGAACATGATGTCGTTGACAAGTTCTTGA

Protein sequence

MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS

Homology

[Close]

BLAST of Carg15752 vs. NCBI nr
Match: KAG7032829.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.5 bits (615), Expect = 3.7e-60
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN
Sbjct: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 118

[Close]

BLAST of Carg15752 vs. NCBI nr
Match: KAG6602129.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 241.5 bits (615), Expect = 3.7e-60
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN
Sbjct: 43  MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 102

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 103 DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Carg15752 vs. NCBI nr
Match: XP_022959484.1 (probable calcium-binding protein CML44 [Cucurbita moschata])

HSP 1 Score: 238.8 bits (608), Expect = 2.4e-59
Identity = 116/118 (98.31%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVD+VYMAFKVFDMN
Sbjct: 43  MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDVVYMAFKVFDMN 102

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSL SS
Sbjct: 103 DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

[Close]

BLAST of Carg15752 vs. NCBI nr
Match: XP_022989807.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 228.0 bits (580), Expect = 4.2e-56
Identity = 111/118 (94.07%), Postives = 114/118 (96.61%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNARE+DVDIVYMAFKVFDMN
Sbjct: 43  MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREDDVDIVYMAFKVFDMN 102

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQ VLSRLE+WDVN +  DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 103 DDGFISCEELQHVLSRLEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Carg15752 vs. NCBI nr
Match: XP_023527806.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.9 bits (572), Expect = 3.5e-55
Identity = 112/118 (94.92%), Postives = 113/118 (95.76%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVS VNAREED+DIVYMAFKVFDMN
Sbjct: 43  MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSHVNAREEDIDIVYMAFKVFDMN 102

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQLVLSRLELWDVN    DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 103 DDGFISCEELQLVLSRLELWDVN---IDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 157

[Close]

BLAST of Carg15752 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 9.8e-16
Identity = 47/113 (41.59%), Postives = 71/113 (62.83%), Query Frame = 0


Query: 2   EDLHSFLEKPSLDFNEFL-FFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 61
           ++L   + K SLD +EFL F+Y+++      + KG   +++   ++ + +  AF VFD+N
Sbjct: 46  DELELIVGKQSLDLDEFLRFYYDAVL-----DSKGSKKNIDVVADNDEAIARAFNVFDVN 105

Query: 62  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMM 114
            DG+IS EEL+ VL RL  ++      DC  MIR +D NLDG +DFEEFKNM+
Sbjct: 106 GDGYISAEELRDVLERLG-FEEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]

BLAST of Carg15752 vs. ExPASy Swiss-Prot
Match: Q5SND2 (Probable calcium-binding protein CML30 OS=Oryza sativa subsp. japonica OX=39947 GN=CML30 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.1e-10
Identity = 45/122 (36.89%), Postives = 60/122 (49.18%), Query Frame = 0


Query: 13  LDFNEFLFFYESISKQNKG--------------ECKGGVSDVNAREEDVDIVYMAFKVFD 72
           +D +EF   Y+SI K+ K               E      +  A EE+ D+   AF VFD
Sbjct: 116 IDIHEFRELYDSIPKRRKSHQQHPLPSTAAADEEAAAADEEYEAEEEERDL-REAFDVFD 175

Query: 73  MNDDGFISCEELQLVLSRLEL--WDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLT 119
            N DG IS EEL  VL  L L          +C+ MIR  D++ DGM+ FEEFK MM++ 
Sbjct: 176 GNKDGLISAEELGTVLESLGLRQHGGRPAVAECRDMIRLVDSDGDGMVSFEEFKRMMTVV 235

[Close]

BLAST of Carg15752 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-09
Identity = 46/122 (37.70%), Postives = 66/122 (54.10%), Query Frame = 0


Query: 1   MEDLHSFLE------KPSLDFNEFLFFYESISKQ-NKGECKGGVS--DVNAREEDVDIVY 60
           + DL S +E         L+F++F   ++++      G C GG +  D ++  E+   + 
Sbjct: 63  LSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLA 122

Query: 61  MAFKVFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKN 114
            AFKVFD N DGFIS  ELQ VL +L L +    +   + MI + D N DG +DF EFKN
Sbjct: 123 EAFKVFDENGDGFISARELQTVLKKLGLPEGGEMER-VEKMIVSVDRNQDGRVDFFEFKN 182

[Close]

BLAST of Carg15752 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-09
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 0


Query: 40  VNAREEDVDIVYMAFKVFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTN 99
           V  +EE+ D +  AF+VFD N DGFI+ EEL+ VL+ + L    T + DCK MI   D +
Sbjct: 70  VEEKEEEED-MREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLE-DCKKMISKVDVD 129

Query: 100 LDGMLDFEEFKNMM 114
            DGM++F+EFK MM
Sbjct: 130 GDGMVNFKEFKQMM 141

[Close]

BLAST of Carg15752 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-09
Identity = 46/118 (38.98%), Postives = 63/118 (53.39%), Query Frame = 0


Query: 2   EDLHSFLEKPSL------DFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFK 61
           +DL   +EK  L      D  EF   Y++I ++              R+E+ D +  AF 
Sbjct: 40  KDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEE--------------RDEEED-MREAFN 99

Query: 62  VFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMM 114
           VFD N DGFI+ EEL+ VL+ L L    T + DCK MI   D + DGM++F+EFK MM
Sbjct: 100 VFDQNRDGFITVEELRSVLASLGLKQGRTLE-DCKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Carg15752 vs. ExPASy TrEMBL
Match: A0A6J1H868 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111460440 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.1e-59
Identity = 116/118 (98.31%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVD+VYMAFKVFDMN
Sbjct: 43  MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDVVYMAFKVFDMN 102

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSL SS
Sbjct: 103 DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

[Close]

BLAST of Carg15752 vs. ExPASy TrEMBL
Match: A0A6J1JGU4 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111486883 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.0e-56
Identity = 111/118 (94.07%), Postives = 114/118 (96.61%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 60
           MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNARE+DVDIVYMAFKVFDMN
Sbjct: 43  MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNAREDDVDIVYMAFKVFDMN 102

Query: 61  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 119
           DDGFISCEELQ VLSRLE+WDVN +  DCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS
Sbjct: 103 DDGFISCEELQHVLSRLEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]

BLAST of Carg15752 vs. ExPASy TrEMBL
Match: A0A6J1CJ36 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111011820 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.0e-44
Identity = 95/125 (76.00%), Postives = 105/125 (84.00%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVS-------DVNAREEDVDIVYMA 60
           ME+L S LEKPSLDFNEFLFFYESISK+NKGEC GGV        D +  EEDVDIVYMA
Sbjct: 42  MEELESSLEKPSLDFNEFLFFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMA 101

Query: 61  FKVFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMM 119
           FKVFDMNDDGFISCEEL+ VL RLELWD N +D++C++MIRAYDTNLDG LDFEEFKNMM
Sbjct: 102 FKVFDMNDDGFISCEELRSVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 161

[Close]

BLAST of Carg15752 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.8e-44
Identity = 92/118 (77.97%), Postives = 103/118 (87.29%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNA-REEDVDIVYMAFKVFDM 60
           ME+L SFLEKPSLDF+EFLFFYESISKQNKGECKGG+ D +   EEDV+IVYMAFKVFD+
Sbjct: 43  MEELESFLEKPSLDFDEFLFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDV 102

Query: 61  NDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTS 118
           N DGFISCEEL+ VLSRLELWD N ++ DC+SMIRAYDTNLDG LDFEEF NMM  T+
Sbjct: 103 NGDGFISCEELENVLSRLELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Carg15752 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 4.4e-43
Identity = 90/118 (76.27%), Postives = 102/118 (86.44%), Query Frame = 0


Query: 1   MEDLHSFLEKPSLDFNEFLFFYESISKQNKGECKGGVSDVNARE-EDVDIVYMAFKVFDM 60
           ME+L SFLEKPSLDF+EFL FYES+SKQNKGECKGG+ D +  E EDV+IVYMAFKVFD+
Sbjct: 43  MEELESFLEKPSLDFDEFLIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDV 102

Query: 61  NDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTS 118
           N DGFISCEEL+ VLSRLELWD N ++ DC+SMIRAYDTNLDG LDFEEF NMM  T+
Sbjct: 103 NGDGFISCEELENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of Carg15752 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 84.3 bits (207), Expect = 7.0e-17
Identity = 47/113 (41.59%), Postives = 71/113 (62.83%), Query Frame = 0


Query: 2   EDLHSFLEKPSLDFNEFL-FFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMN 61
           ++L   + K SLD +EFL F+Y+++      + KG   +++   ++ + +  AF VFD+N
Sbjct: 46  DELELIVGKQSLDLDEFLRFYYDAVL-----DSKGSKKNIDVVADNDEAIARAFNVFDVN 105

Query: 62  DDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMM 114
            DG+IS EEL+ VL RL  ++      DC  MIR +D NLDG +DFEEFKNM+
Sbjct: 106 GDGYISAEELRDVLERLG-FEEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]

BLAST of Carg15752 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 63.9 bits (154), Expect = 9.7e-11
Identity = 46/122 (37.70%), Postives = 66/122 (54.10%), Query Frame = 0


Query: 1   MEDLHSFLE------KPSLDFNEFLFFYESISKQ-NKGECKGGVS--DVNAREEDVDIVY 60
           + DL S +E         L+F++F   ++++      G C GG +  D ++  E+   + 
Sbjct: 63  LSDLKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLA 122

Query: 61  MAFKVFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKN 114
            AFKVFD N DGFIS  ELQ VL +L L +    +   + MI + D N DG +DF EFKN
Sbjct: 123 EAFKVFDENGDGFISARELQTVLKKLGLPEGGEMER-VEKMIVSVDRNQDGRVDFFEFKN 182

[Close]

BLAST of Carg15752 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-10
Identity = 46/118 (38.98%), Postives = 63/118 (53.39%), Query Frame = 0


Query: 2   EDLHSFLEKPSL------DFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFK 61
           +DL   +EK  L      D  EF   Y++I ++              R+E+ D +  AF 
Sbjct: 40  KDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEE--------------RDEEED-MREAFN 99

Query: 62  VFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMM 114
           VFD N DGFI+ EEL+ VL+ L L    T + DCK MI   D + DGM++F+EFK MM
Sbjct: 100 VFDQNRDGFITVEELRSVLASLGLKQGRTLE-DCKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Carg15752 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-10
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 0


Query: 40  VNAREEDVDIVYMAFKVFDMNDDGFISCEELQLVLSRLELWDVNTTDTDCKSMIRAYDTN 99
           V  +EE+ D +  AF+VFD N DGFI+ EEL+ VL+ + L    T + DCK MI   D +
Sbjct: 70  VEEKEEEED-MREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLE-DCKKMISKVDVD 129

Query: 100 LDGMLDFEEFKNMM 114
            DGM++F+EFK MM
Sbjct: 130 GDGMVNFKEFKQMM 141

[Close]

BLAST of Carg15752 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 60.8 bits (146), Expect = 8.2e-10
Identity = 40/101 (39.60%), Postives = 56/101 (55.45%), Query Frame = 0


Query: 13  LDFNEFLFFYESISKQNKGECKGGVSDVNAREEDVDIVYMAFKVFDMNDDGFISCEELQL 72
           +D +EF   Y++I  +   E           EED+     AF VFD N DGFI+ +EL+ 
Sbjct: 57  VDIDEFGELYKTIMDEEDEE-----------EEDMK---EAFNVFDQNGDGFITVDELKA 116

Query: 73  VLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMM 114
           VLS L L    T D DCK MI+  D + DG ++++EF+ MM
Sbjct: 117 VLSSLGLKQGKTLD-DCKKMIKKVDVDGDGRVNYKEFRQMM 142

The following BLAST results are available for this feature:

BLAST of Carg15752 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032829.1	3.7e-60	100.00	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. a...	[more]
KAG6602129.1	3.7e-60	100.00	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022959484.1	2.4e-59	98.31	probable calcium-binding protein CML44 [Cucurbita moschata]	[more]
XP_022989807.1	4.2e-56	94.07	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]
XP_023527806.1	3.5e-55	94.92	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg15752 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	9.8e-16	41.59	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q5SND2	6.1e-10	36.89	Probable calcium-binding protein CML30 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SVG9	1.4e-09	37.70	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9SU00	2.3e-09	48.65	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9SRR7	2.3e-09	38.98	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of Carg15752 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H868	1.1e-59	98.31	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1JGU4	2.0e-56	94.07	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114868...	[more]
A0A6J1CJ36	1.0e-44	76.00	probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FLU5	1.8e-44	77.97	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1K1Y7	4.4e-43	76.27	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]

BLAST of Carg15752 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	7.0e-17	41.59	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	9.7e-11	37.70	calmodulin like 42	[more]
AT3G07490.1	1.7e-10	38.98	ARF-GAP domain 11	[more]
AT4G12860.1	1.7e-10	48.65	EF hand calcium-binding protein family	[more]
AT1G05990.1	8.2e-10	39.60	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 49..77 e-value: 0.32 score: 20.1 coord: 88..116 e-value: 3.9E-4 score: 29.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 51..114 e-value: 3.6E-10 score: 40.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 84..118 score: 11.584389
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 45..80 score: 11.863342
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 53..114 e-value: 8.17465E-13 score: 56.7873
None	No IPR available	GENE3D	1.10.238.10		coord: 16..114 e-value: 3.1E-19 score: 71.0
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..118
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 2..118
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 58..70
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 11..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15752-RA	Carg15752-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding