Carg13298 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13298
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mitoferrin-like
Location	Carg_Chr07: 6169886 .. 6170908 (-)
RNA-Seq Expression	Carg13298
Synteny	Carg13298

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAACAACCGTCTCGCACTCTCCCGATTATCGCCCCGAAATTTCAGTAATCCCGCCCACCCGCGACGGCCTGTCCTTCTGGCAGTTCATGATCGCCGGATCAATTGCAGGGTCTGTCGAGCACATGGCCATGTTTCCAGTGGACACACTCAAGACCCGAATCCAAGCCCTTGCCGGATCATCCACCCTCCGACAAGCACTCAGTTCAATTCTCAAATCGGAAGGTCCGGCCGGGCTCTACCGTGGAATCGGAGCAATGGGTCTCGGAGCAGGACCTGCACACGCCGTATACTTCTCTGTATACGAATTCTGCAAACAAAGATTTTCGATTGGGAATCCCAACAACCCATTAGCCCACGCCATTTCTGGAGTCTGTGCAACGGTGGCGAGCGACGCCGCGTTGACGCCGATGGATGTGGTGAAACAGAGGCTGCAATTGAAGAGCAGTCCTTATAAGGGAGTGAGGGACTGTGCGAGGAGGATTCTGGTCGAGGAAGGAATTGGGGCGTTCTATGCTTCTTATTGGACTACTGTTTTGATGAATGCCCCGTACACGGCTGCCCATTTTGCCATTTATGAAGCTGTGAAAAGGGGTTTGAAGGAGGGCTCGCCGGGAAGCGAAGGCGGGGAGGGGTTGGTTGTCCATGCTACGGCTGGTGCGGCTGCTGGGTCTCTTGCTGCTGGTCTTACAACGCCATTAGACGTAGTGAAAACGCGGCTGCAGTGCCAGGTAATGTGTGGTTGTGCTTCTTGTTGGCCTCGAGAGGGGTCCTCTGTTTTAGCATGGCTAAGGCAATGGTTTTAGTGTACAAAACAGGGAGTGTGTGGGTGCGACAGATTTTCAAGCAGTTCAATAATATCTGTGGTTAGGCATGTGGTGAAGAAGGATGGGAAGAGCGGGTTGATGAGAGGGTGGATTCCAAGGATGCTGTTCCATGCGCCTGCTGCTGCCATTGCCTGGTCCACTTATGAAGCCTCAAAGACATTGTTCCAACACCTCCACAATCACAACAACAATTAG

mRNA sequence

ATGACAACAACCGTCTCGCACTCTCCCGATTATCGCCCCGAAATTTCAGTAATCCCGCCCACCCGCGACGGCCTGTCCTTCTGGCAGTTCATGATCGCCGGATCAATTGCAGGGTCTGTCGAGCACATGGCCATGTTTCCAGTGGACACACTCAAGACCCGAATCCAAGCCCTTGCCGGATCATCCACCCTCCGACAAGCACTCAGTTCAATTCTCAAATCGGAAGGTCCGGCCGGGCTCTACCGTGGAATCGGAGCAATGGGTCTCGGAGCAGGACCTGCACACGCCGTATACTTCTCTGTATACGAATTCTGCAAACAAAGATTTTCGATTGGGAATCCCAACAACCCATTAGCCCACGCCATTTCTGGAGTCTGTGCAACGGTGGCGAGCGACGCCGCGTTGACGCCGATGGATGTGGTGAAACAGAGGCTGCAATTGAAGAGCAGTCCTTATAAGGGAGTGAGGGACTGTGCGAGGAGGATTCTGGTCGAGGAAGGAATTGGGGCGTTCTATGCTTCTTATTGGACTACTGTTTTGATGAATGCCCCGTACACGGCTGCCCATTTTGCCATTTATGAAGCTGTGAAAAGGGGTTTGAAGGAGGGCTCGCCGGGAAGCGAAGGCGGGGAGGGGTTGGTTGTCCATGCTACGGCTGGTGCGGCTGCTGGGTCTCTTGCTGCTGGTCTTACAACGCCATTAGACGTAGTGAAAACGCGGCTGCAGTGCCAGGGAGTGTGTGGGTGCGACAGATTTTCAAGCAGTTCAATAATATCTGTGGTTAGGCATGTGGTGAAGAAGGATGGGAAGAGCGGGTTGATGAGAGGGTGGATTCCAAGGATGCTGTTCCATGCGCCTGCTGCTGCCATTGCCTGGTCCACTTATGAAGCCTCAAAGACATTGTTCCAACACCTCCACAATCACAACAACAATTAG

Coding sequence (CDS)

ATGACAACAACCGTCTCGCACTCTCCCGATTATCGCCCCGAAATTTCAGTAATCCCGCCCACCCGCGACGGCCTGTCCTTCTGGCAGTTCATGATCGCCGGATCAATTGCAGGGTCTGTCGAGCACATGGCCATGTTTCCAGTGGACACACTCAAGACCCGAATCCAAGCCCTTGCCGGATCATCCACCCTCCGACAAGCACTCAGTTCAATTCTCAAATCGGAAGGTCCGGCCGGGCTCTACCGTGGAATCGGAGCAATGGGTCTCGGAGCAGGACCTGCACACGCCGTATACTTCTCTGTATACGAATTCTGCAAACAAAGATTTTCGATTGGGAATCCCAACAACCCATTAGCCCACGCCATTTCTGGAGTCTGTGCAACGGTGGCGAGCGACGCCGCGTTGACGCCGATGGATGTGGTGAAACAGAGGCTGCAATTGAAGAGCAGTCCTTATAAGGGAGTGAGGGACTGTGCGAGGAGGATTCTGGTCGAGGAAGGAATTGGGGCGTTCTATGCTTCTTATTGGACTACTGTTTTGATGAATGCCCCGTACACGGCTGCCCATTTTGCCATTTATGAAGCTGTGAAAAGGGGTTTGAAGGAGGGCTCGCCGGGAAGCGAAGGCGGGGAGGGGTTGGTTGTCCATGCTACGGCTGGTGCGGCTGCTGGGTCTCTTGCTGCTGGTCTTACAACGCCATTAGACGTAGTGAAAACGCGGCTGCAGTGCCAGGGAGTGTGTGGGTGCGACAGATTTTCAAGCAGTTCAATAATATCTGTGGTTAGGCATGTGGTGAAGAAGGATGGGAAGAGCGGGTTGATGAGAGGGTGGATTCCAAGGATGCTGTTCCATGCGCCTGCTGCTGCCATTGCCTGGTCCACTTATGAAGCCTCAAAGACATTGTTCCAACACCTCCACAATCACAACAACAATTAG

Protein sequence

MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAHAISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVLMNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTRLQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKTLFQHLHNHNNN

Homology

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BLAST of Carg13298 vs. NCBI nr
Match: KAG7027417.1 (Mitoferrin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNNN 312
           LFQHLHNHNNN
Sbjct: 301 LFQHLHNHNNN 311

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BLAST of Carg13298 vs. NCBI nr
Match: KAG6595407.1 (Mitoferrin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 621.3 bits (1601), Expect = 4.5e-174
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNN 311
           LFQHLHNHNN
Sbjct: 301 LFQHLHNHNN 310

[Close]

BLAST of Carg13298 vs. NCBI nr
Match: XP_022931904.1 (mitoferrin-like [Cucurbita moschata])

HSP 1 Score: 619.0 bits (1595), Expect = 2.2e-173
Identity = 309/310 (99.68%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNN 311
           LFQHLHN+NN
Sbjct: 301 LFQHLHNYNN 310

[Close]

BLAST of Carg13298 vs. NCBI nr
Match: XP_023517443.1 (mitoferrin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.3 bits (1575), Expect = 4.6e-171
Identity = 303/310 (97.74%), Postives = 309/310 (99.68%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTV+HSPDYRPEISVIPPTRDGLSFWQFMIAGSI+GSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVTHSPDYRPEISVIPPTRDGLSFWQFMIAGSISGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQAL+SILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALASILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHF+IYEAVKRGLKEGSPGS+GGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFSIYEAVKRGLKEGSPGSDGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDRFSSSSI SVVRHVV+KDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRFSSSSIRSVVRHVVEKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNN 311
           LFQHLHNHNN
Sbjct: 301 LFQHLHNHNN 310

[Close]

BLAST of Carg13298 vs. NCBI nr
Match: XP_022966611.1 (mitoferrin-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 600.9 bits (1548), Expect = 6.3e-168
Identity = 299/310 (96.45%), Postives = 303/310 (97.74%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTV+HSPD RPEISV PPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVTHSPDCRPEISVFPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQAL+SILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALASILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFY SYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYTSYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGS+GGEGLVVH TAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSDGGEGLVVHVTAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDR SSSSI +VVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRLSSSSITNVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNN 311
           LFQHLHN NN
Sbjct: 301 LFQHLHNDNN 310

[Close]

BLAST of Carg13298 vs. ExPASy Swiss-Prot
Match: Q55DY8 (Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 126/297 (42.42%), Postives = 181/297 (60.94%), Query Frame = 0


Query: 23  DGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL---AGSSTLRQALSSILKSEGPAG 82
           +G SF+  +IAG+ AG  EH  M+P+DT+KT IQA+   A  ++  Q    I++  G  G
Sbjct: 12  EGGSFYVHLIAGAAAGFAEHCGMYPIDTIKTHIQAIKPGAMQTSSLQITKHIIQQHGITG 71

Query: 83  LYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGN--PNNPLAHAISGVCATVASDAALTP 142
           L+RG+ A+  GA P+HAV+FS+YE  K +F IG+   ++P+   I+G  AT+ S+A  +P
Sbjct: 72  LFRGLTAVAAGAAPSHAVHFSIYELLKFKF-IGSDEDHHPIKVGIAGAIATMTSEAVASP 131

Query: 143 MDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVLMNAPYTAAHFAIYEAVK 202
           MDVVKQRLQL+ + YKG+ DC +RI V+EGI  FY+ Y TT++MN PY   +FA YE++K
Sbjct: 132 MDVVKQRLQLQITDYKGLTDCTKRIWVKEGIRGFYSGYTTTLVMNVPYNIVYFASYESLK 191

Query: 203 RGLK---EGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTRLQCQGVCGCDRFSS 262
           + ++         E    L+ H  AG  AG LAA  T P DVVKTRLQ Q     D  +S
Sbjct: 192 KIIQPWFNNKNPEERSYQLIDHLVAGGGAGMLAAAFTNPFDVVKTRLQTQS----DFIAS 251

Query: 263 SSI------------ISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASK 300
           S+I            +  ++ +  ++G  G +RG  PRM+FH+ ++AI WS YE  K
Sbjct: 252 STINSAKSIKRYGGMMDAMKTIWIEEGMDGYLRGMKPRMVFHSMSSAIVWSVYEYFK 303

[Close]

BLAST of Carg13298 vs. ExPASy Swiss-Prot
Match: O14281 (Uncharacterized mitochondrial carrier C8C9.12c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC8C9.12c PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.5e-51
Identity = 111/284 (39.08%), Postives = 160/284 (56.34%), Query Frame = 0


Query: 24  GLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG-----SSTLRQALSSILKSEGPA 83
           G   +  ++AG+ +G +EH  M+PVD +KTR+Q L G     S  +  ++  I  +EG  
Sbjct: 16  GSPMYAHLLAGAFSGILEHSVMYPVDAIKTRMQMLNGVSRSVSGNIVNSVIKISSTEGVY 75

Query: 84  GLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAHAISGVCATVASDAALTPM 143
            L+RGI ++ +GAGP+HA+YFSV EF K + +  +P+ PLA A++G CA   SDA +TP 
Sbjct: 76  SLWRGISSVIMGAGPSHAIYFSVLEFFKSKIN-ASPDRPLASALAGACAITISDAFMTPF 135

Query: 144 DVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVLMNAPYTAAHFAIYEAVKR 203
           DV+KQR+QL S  YK    CA  +   EG+GAFY SY T + M+ P+TA   A Y+    
Sbjct: 136 DVIKQRMQLPSRKYKSALHCATTVFRNEGLGAFYISYPTCIAMSIPFTAIQVATYDTCMS 195

Query: 204 GLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTRLQCQGVCGCDRF-SSSSI 263
            L   +           H  +G  +G++A+ LTTPLDVVKT LQ +G             
Sbjct: 196 FLNPNAVYDP-----TSHIISGGLSGAIASSLTTPLDVVKTLLQTRGSSSIPEVRKCKGS 255

Query: 264 ISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKTL 302
           + VVR +    G     +G  PRM+   PA A++W+ YEA K +
Sbjct: 256 LDVVRFIYNYGGIPSFFKGIRPRMVVAMPATAVSWAAYEAGKEI 293

[Close]

BLAST of Carg13298 vs. ExPASy Swiss-Prot
Match: P10566 (Mitochondrial RNA-splicing protein MRS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MRS3 PE=1 SV=4)

HSP 1 Score: 199.1 bits (505), Expect = 7.2e-50
Identity = 119/284 (41.90%), Postives = 166/284 (58.45%), Query Frame = 0


Query: 31  MIAGSIAGSVEHMAMFPVDTLKTRIQALAGSS----TLRQALSSILKSEGPAGLYRGIGA 90
           +IAG+ AG +EH  MFP+D LKTRIQ+    S     +   +S I  SEG   L++G+ +
Sbjct: 37  LIAGAFAGIMEHSVMFPIDALKTRIQSANAKSLSAKNMLSQISHISTSEGTLALWKGVQS 96

Query: 91  MGLGAGPAHAVYFSVYEFCKQRF---SIGNPNNPLAHAISGVCATVASDAALTPMDVVKQ 150
           + LGAGPAHAVYF  YEFCK+     S    ++P   AISG CAT ASDA + P D +KQ
Sbjct: 97  VILGAGPAHAVYFGTYEFCKKNLIDSSDTQTHHPFKTAISGACATTASDALMNPFDTIKQ 156

Query: 151 RLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVLMNAPYTAAHFAIYEAVKRGLKEG 210
           R+QL +S    V    ++I   EG+ AFY SY TT++MN P+ A +F IYE+  + L   
Sbjct: 157 RIQLNTS--ASVWQTTKQIYQSEGLAAFYYSYPTTLVMNIPFAAFNFVIYESSTKFL--- 216

Query: 211 SPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTRLQCQG--------VCGCDRFSSS 270
           +P +E     ++H   G+ +GS  A +TTPLD +KT LQ +G        +   D FS +
Sbjct: 217 NPSNEYNP--LIHCLCGSISGSTCAAITTPLDCIKTVLQIRGSQTVSLEIMRKADTFSKA 276

Query: 271 SIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASK 300
           +  S +  V    G  G  RGW PR++ + PA AI+W+ YE +K
Sbjct: 277 A--SAIYQVY---GWKGFWRGWKPRIVANMPATAISWTAYECAK 308

[Close]

BLAST of Carg13298 vs. ExPASy Swiss-Prot
Match: Q96A46 (Mitoferrin-2 OS=Homo sapiens OX=9606 GN=SLC25A28 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.2e-48
Identity = 119/311 (38.26%), Postives = 169/311 (54.34%), Query Frame = 0


Query: 5   VSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSSTL 64
           V   PD  P+   +P    G +    M+AG++AG +EH  M+P+D +KTR+Q+L      
Sbjct: 53  VRQDPDSGPDYEALPA---GATVTTHMVAGAVAGILEHCVMYPIDCVKTRMQSLQPDPAA 112

Query: 65  R-----QALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFS-IGNP--NN 124
           R     +AL  I+++EG     RG+     GAGPAHA+YF+ YE  K+  S + +P  N+
Sbjct: 113 RYRNVLEALWRIIRTEGLWRPMRGLNVTATGAGPAHALYFACYEKLKKTLSDVIHPGGNS 172

Query: 125 PLAHAISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYW 184
            +A+  +G  AT+  DAA+ P +VVKQR+Q+ +SPY  V DC R +   EG GAFY SY 
Sbjct: 173 HIANGAAGCVATLLHDAAMNPAEVVKQRMQMYNSPYHRVTDCVRAVWQNEGAGAFYRSYT 232

Query: 185 TTVLMNAPYTAAHFAIYEAVKRGL---KEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTP 244
           T + MN P+ A HF  YE ++      +  +P S        H  +GA AG++AA  TTP
Sbjct: 233 TQLTMNVPFQAIHFMTYEFLQEHFNPQRRYNPSS--------HVLSGACAGAVAAAATTP 292

Query: 245 LDVVKTRLQCQGVCGCDRFSSSSI---ISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAI 302
           LDV KT L  Q     +   +  I    S  R V +  G +   RG   R+++  P+ AI
Sbjct: 293 LDVCKTLLNTQESLALNSHITGHITGMASAFRTVYQVGGVTAYFRGVQARVIYQIPSTAI 352

[Close]

BLAST of Carg13298 vs. ExPASy Swiss-Prot
Match: Q8R0Z5 (Mitoferrin-2 OS=Mus musculus OX=10090 GN=Slc25a28 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.4e-47
Identity = 118/307 (38.44%), Postives = 167/307 (54.40%), Query Frame = 0


Query: 9   PDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSSTLR--- 68
           P+  PE   +P    G +    M+AG++AG +EH  M+P+D +KTR+Q+L      R   
Sbjct: 57  PESGPEYEALPA---GATVTTHMVAGAVAGILEHCVMYPIDCVKTRMQSLQPDPAARYRN 116

Query: 69  --QALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFS-IGNP--NNPLAH 128
             +AL  I+++EG     RG+     GAGPAHA+YF+ YE  K+  S + +P  N+ +A+
Sbjct: 117 VLEALWRIMRTEGLWRPMRGLNVTATGAGPAHALYFACYEKLKKTLSDVIHPGGNSHIAN 176

Query: 129 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 188
             +G  AT+  DAA+ P +VVKQR+Q+ +SPY  V DC R +   EG GAFY SY T + 
Sbjct: 177 GAAGCVATLLHDAAMNPAEVVKQRMQMYNSPYHRVTDCVRAVWQNEGAGAFYRSYTTQLT 236

Query: 189 MNAPYTAAHFAIYEAVKRGL---KEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVV 248
           MN P+ A HF  YE ++      +  +P S        H   GA AG++AA  TTPLDV 
Sbjct: 237 MNVPFQAIHFMTYEFLQEHFNPQRRYNPSS--------HVLCGACAGAVAAAATTPLDVC 296

Query: 249 KTRLQCQGVCGCDRFSSSSI---ISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWST 302
           KT L  Q     +   +  I    S  R V +  G +   RG   R+++  P+ AIAWS 
Sbjct: 297 KTLLNTQESLALNSNITGHITGMASAFRTVYQVGGVTAYFRGVQARVIYQIPSTAIAWSV 352

[Close]

BLAST of Carg13298 vs. ExPASy TrEMBL
Match: A0A6J1EVI8 (mitoferrin-like OS=Cucurbita moschata OX=3662 GN=LOC111438181 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.1e-173
Identity = 309/310 (99.68%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNN 311
           LFQHLHN+NN
Sbjct: 301 LFQHLHNYNN 310

[Close]

BLAST of Carg13298 vs. ExPASy TrEMBL
Match: A0A6J1HPT3 (mitoferrin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466240 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.0e-168
Identity = 299/310 (96.45%), Postives = 303/310 (97.74%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTV+HSPD RPEISV PPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVTHSPDCRPEISVFPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQAL+SILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALASILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFY SYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYTSYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGS+GGEGLVVH TAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSDGGEGLVVHVTAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDR SSSSI +VVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRLSSSSITNVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHNHNN 311
           LFQHLHN NN
Sbjct: 301 LFQHLHNDNN 310

[Close]

BLAST of Carg13298 vs. ExPASy TrEMBL
Match: A0A6J1HNG7 (mitoferrin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466240 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.3e-167
Identity = 297/307 (96.74%), Postives = 301/307 (98.05%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60
           MTTTV+HSPD RPEISV PPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG
Sbjct: 1   MTTTVTHSPDCRPEISVFPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAG 60

Query: 61  SSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120
           SSTLRQAL+SILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH
Sbjct: 61  SSTLRQALASILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 120

Query: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 180
           AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFY SYWTTVL
Sbjct: 121 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYTSYWTTVL 180

Query: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTR 240
           MNAPYTAAHFAIYEAVKRGLKEGSPGS+GGEGLVVH TAGAAAGSLAAGLTTPLDVVKTR
Sbjct: 181 MNAPYTAAHFAIYEAVKRGLKEGSPGSDGGEGLVVHVTAGAAAGSLAAGLTTPLDVVKTR 240

Query: 241 LQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300
           LQCQGVCGCDR SSSSI +VVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT
Sbjct: 241 LQCQGVCGCDRLSSSSITNVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKT 300

Query: 301 LFQHLHN 308
           LFQHLHN
Sbjct: 301 LFQHLHN 307

[Close]

BLAST of Carg13298 vs. ExPASy TrEMBL
Match: A0A0A0L006 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G312860 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-144
Identity = 261/311 (83.92%), Postives = 280/311 (90.03%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-A 60
           M T+VSHSPD+RPE+SV PPT DGL FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL  
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLA 120
           GSST+RQAL SILK EGPAGLYRGIGAMGLGAGPAHAVYFSVYEF K+ FS+GN NNPLA
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTV 180
           HAI+GVCATV SDA LTPMDVVKQRLQLKSSPYKGVRDC +RILVEEGIGA YASY TTV
Sbjct: 121 HAIAGVCATVTSDAVLTPMDVVKQRLQLKSSPYKGVRDCVKRILVEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKT 240
           +MNAPYTA +FA YEA KRGLKE SPGS+  E L+VHATAGAAAGSLAA LTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDEDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASK 300
           RLQCQGVCGCD+FSSSSI  V+  VVKKDG +GLM+GWIPRM+FHAPAAAI WSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TLFQHLHNHNN 311
           + FQHLHNHNN
Sbjct: 301 SFFQHLHNHNN 311

[Close]

BLAST of Carg13298 vs. ExPASy TrEMBL
Match: A0A5A7T6D3 (Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00190 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.4e-143
Identity = 258/312 (82.69%), Postives = 279/312 (89.42%), Query Frame = 0


Query: 1   MTTTVSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQAL-A 60
           M T+VSHSPD+RPE+SV PPT DGL FWQFMIAGSIAGSVEHMAM+PVDTLKTRIQAL  
Sbjct: 1   MATSVSHSPDFRPEVSVTPPTHDGLYFWQFMIAGSIAGSVEHMAMYPVDTLKTRIQALGG 60

Query: 61  GSSTLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLA 120
           GSST+RQAL SILK EGPAGLYRGIGAMGLGAGPAHAVYFSVYEF K+ FS+GN NNPLA
Sbjct: 61  GSSTVRQALGSILKVEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFAKEGFSMGNKNNPLA 120

Query: 121 HAISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTV 180
           HAI+GVCATV SDA +TPMDVVKQRLQL++SPYKGV DC RRIL EEGIGA YASY TTV
Sbjct: 121 HAIAGVCATVTSDAVITPMDVVKQRLQLENSPYKGVGDCVRRILAEEGIGALYASYRTTV 180

Query: 181 LMNAPYTAAHFAIYEAVKRGLKEGSPGSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKT 240
           +MNAPYTA +FA YEA KRGLKE SPGS+  E L+VHATAGAAAGSLAA LTTPLDVVKT
Sbjct: 181 VMNAPYTAVYFATYEAAKRGLKEVSPGSDDDERLIVHATAGAAAGSLAAALTTPLDVVKT 240

Query: 241 RLQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASK 300
           RLQCQGVCGCD+FSSSSI  V+  VVKKDG +GLM+GWIPRM+FHAPAAAI WSTYEASK
Sbjct: 241 RLQCQGVCGCDKFSSSSIGYVLGCVVKKDGYNGLMKGWIPRMMFHAPAAAICWSTYEASK 300

Query: 301 TLFQHLHNHNNN 312
           + FQHLHNHNNN
Sbjct: 301 SFFQHLHNHNNN 312

[Close]

BLAST of Carg13298 vs. TAIR 10
Match: AT1G07030.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 405.6 bits (1041), Expect = 3.6e-113
Identity = 208/311 (66.88%), Postives = 242/311 (77.81%), Query Frame = 0


Query: 5   VSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSST- 64
           VS +PD++PEI+      DGL FWQFMIAGSIAGSVEHMAMFPVDT+KT +QAL      
Sbjct: 19  VSQTPDFKPEIA-----HDGLKFWQFMIAGSIAGSVEHMAMFPVDTIKTHMQALRPCPLK 78

Query: 65  ---LRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 124
              +R+A  SI++ EGP+ LYRGI AMGLGAGPAHAVYFS YE  K+  S G+ NN +AH
Sbjct: 79  PVGIREAFRSIIQKEGPSALYRGIWAMGLGAGPAHAVYFSFYEVSKKYLSAGDQNNSVAH 138

Query: 125 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 184
           A+SGV AT++SDA  TPMD+VKQRLQ+    YKGV DC +R+L EEGIGAFYASY TTVL
Sbjct: 139 AMSGVFATISSDAVFTPMDMVKQRLQMGEGTYKGVWDCVKRVLREEGIGAFYASYRTTVL 198

Query: 185 MNAPYTAAHFAIYEAVKRGLKEGSPGS-EGGEGLVVHATAGAAAGSLAAGLTTPLDVVKT 244
           MNAP+TA HFA YEA K+GL E SP      EG +VHATAGAAAG LAA +TTPLDVVKT
Sbjct: 199 MNAPFTAVHFATYEAAKKGLMEFSPDRISDEEGWLVHATAGAAAGGLAAAVTTPLDVVKT 258

Query: 245 RLQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASK 304
           +LQCQGVCGCDRF+SSSI  V+R +VKKDG  GL+RGW+PRMLFHAPAAAI WSTYE  K
Sbjct: 259 QLQCQGVCGCDRFTSSSISHVLRTIVKKDGYRGLLRGWLPRMLFHAPAAAICWSTYEGVK 318

Query: 305 TLFQHLHNHNN 311
           + FQ  +  +N
Sbjct: 319 SFFQDFNVDSN 324

[Close]

BLAST of Carg13298 vs. TAIR 10
Match: AT2G30160.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 1.5e-111
Identity = 205/314 (65.29%), Postives = 237/314 (75.48%), Query Frame = 0


Query: 5   VSHSPDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSST- 64
           +   PD+ P I ++P     L FWQ M+AGSIAGSVEHMAMFPVDT+KT +QAL      
Sbjct: 17  IPQPPDFHPAI-IVPAQNTTLKFWQLMVAGSIAGSVEHMAMFPVDTVKTHMQALRSCPIK 76

Query: 65  ---LRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPNNPLAH 124
              +RQA  SI+K++GP+ LYRGI AMGLGAGPAHAVYFS YE  K+  S GNPNN  AH
Sbjct: 77  PIGIRQAFRSIIKTDGPSALYRGIWAMGLGAGPAHAVYFSFYEVSKKFLSGGNPNNSAAH 136

Query: 125 AISGVCATVASDAALTPMDVVKQRLQLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVL 184
           AISGV AT++SDA  TPMD+VKQRLQ+ +  YKGV DC +R+  EEG GAFYASY TTVL
Sbjct: 137 AISGVFATISSDAVFTPMDMVKQRLQIGNGTYKGVWDCIKRVTREEGFGAFYASYRTTVL 196

Query: 185 MNAPYTAAHFAIYEAVKRGLKEGSP----GSEGGEGLVVHATAGAAAGSLAAGLTTPLDV 244
           MNAP+TA HF  YEAVKRGL+E  P    G+E  EG +++ATAGAAAG LAA +TTPLDV
Sbjct: 197 MNAPFTAVHFTTYEAVKRGLREMLPEHAVGAEDEEGWLIYATAGAAAGGLAAAVTTPLDV 256

Query: 245 VKTRLQCQGVCGCDRFSSSSIISVVRHVVKKDGKSGLMRGWIPRMLFHAPAAAIAWSTYE 304
           VKT+LQCQGVCGCDRF SSSI  V R +VKKDG  GL RGW+PRMLFHAPAAAI WSTYE
Sbjct: 257 VKTQLQCQGVCGCDRFKSSSISDVFRTIVKKDGYRGLARGWLPRMLFHAPAAAICWSTYE 316

Query: 305 ASKTLFQHLHNHNN 311
             K+ FQ L+   N
Sbjct: 317 TVKSFFQDLNGEAN 329

[Close]

BLAST of Carg13298 vs. TAIR 10
Match: AT1G74240.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 139.0 bits (349), Expect = 6.3e-33
Identity = 102/340 (30.00%), Postives = 150/340 (44.12%), Query Frame = 0


Query: 9   PDYRPEISVIPPTRDGLSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQ------ALAGSS 68
           P +R  +  I  T D    W+  + G IAG+     M PVDTLKTR+Q      A     
Sbjct: 15  PSFRKSVE-IKATHDQFFVWREFLWGGIAGAFGEGMMHPVDTLKTRLQSQIIMNATQRQK 74

Query: 69  TLRQALSSILKSEGPAGLYRGIGAMGLGAGPAHAVYFSVYEFCKQRFSIGNPN--NPLAH 128
           ++ Q L ++   +G  G YRGI     G+    A YF   E  K+     +P+     AH
Sbjct: 75  SILQMLRTVWVGDGLKGFYRGIAPGVTGSLATGATYFGFIESTKKWIEESHPSLAGHWAH 134

Query: 129 AISGVCATVASDAALTPMDVVKQRLQLKSSP-----------------------YKGVRD 188
            I+G            P +V+KQR+Q++ +                        Y G+  
Sbjct: 135 FIAGAVGDTLGSFIYVPCEVIKQRMQIQGTSSSWSSYISRNSVPVQPRGDMYGYYTGMFQ 194

Query: 189 CARRILVEEGIGAFYASYWTTVLMNAPYTAAHFAIYEAVKRGLKEGSPG------SEGGE 248
               I  E+G    YA YW+T+  + P+       YE +K    +G         +   E
Sbjct: 195 AGCSIWKEQGPKGLYAGYWSTLARDVPFAGLMVVFYEGLKDLTDQGKKKFPQYGVNSSIE 254

Query: 249 GLVVHATAGAAAGSLAAGLTTPLDVVKTRLQCQGVCGCDRFSSSSIISVVRHVVKKDGKS 308
           GLV+    G  AG L+A LTTPLDVVKTRLQ QG            +  V  + +K+G  
Sbjct: 255 GLVL----GGLAGGLSAYLTTPLDVVKTRLQVQG----STIKYKGWLDAVGQIWRKEGPQ 314

Query: 309 GLMRGWIPRMLFHAPAAAIAWSTYEASKTLFQHLHNHNNN 312
           G  RG +PR++++ PA+A+ +   E  +  F+   N+NNN
Sbjct: 315 GFFRGSVPRVMWYLPASALTFMAVEFLRDNFREKSNNNNN 345

[Close]

BLAST of Carg13298 vs. TAIR 10
Match: AT1G34065.1 (S-adenosylmethionine carrier 2 )

HSP 1 Score: 136.7 bits (343), Expect = 3.1e-32
Identity = 91/286 (31.82%), Postives = 142/286 (49.65%), Query Frame = 0


Query: 28  WQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSSTLRQALSSILKSEGPAGLYRGIGAM 87
           ++ +I G +AG V   A++P+DT+KTRIQ        R     I K     GLY G+G  
Sbjct: 79  YESLITGGLAGVVVEAALYPIDTIKTRIQ------VARDGGKIIWK-----GLYSGLGGN 138

Query: 88  GLGAGPAHAVYFSVYEFCKQRFSIGNPNN--PLAHAISGVCATVASDAALTPMDVVKQRL 147
            +G  PA A++F VYE  KQ+     P+N   +AH  +G      S     P +VVKQR+
Sbjct: 139 LVGVLPASALFFGVYEPTKQKLLKVLPDNLSAVAHLAAGALGGAVSSIVRVPTEVVKQRM 198

Query: 148 QLKSSPYKGVRDCARRILVEEGIGAFYASYWTTVLMNAPYTAAHFAIYEAVKRGLKEGSP 207
           Q  +  +    D  R I+ +EG G  YA Y + +L + P+ A  F +YE ++ G K  + 
Sbjct: 199 Q--TGQFVSAPDAVRLIIAKEGFGGMYAGYGSFLLRDLPFDALQFCVYEQLRIGYKLAAR 258

Query: 208 GSEGGEGLVVHATAGAAAGSLAAGLTTPLDVVKTRLQCQGVCGCDRFSSSSIISVVRHVV 267
                     +A  GA AG++   LTTPLDV+KTRL  QG           +   ++ ++
Sbjct: 259 RDLNDP---ENAMIGAFAGAVTGVLTTPLDVIKTRLMVQG----SGTQYKGVSDCIKTII 318

Query: 268 KKDGKSGLMRGWIPRMLFHAPAAAIAWSTYEASKTLFQHLHNHNNN 312
           +++G S L +G  PR+L+     +I +   E +K +       ++N
Sbjct: 319 REEGSSALWKGMGPRVLWIGIGGSIFFGVLEKTKQILSERSQKSHN 344

[Close]

BLAST of Carg13298 vs. TAIR 10
Match: AT1G07025.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 134.0 bits (336), Expect = 2.0e-31
Identity = 76/151 (50.33%), Postives = 94/151 (62.25%), Query Frame = 0


Query: 25  LSFWQFMIAGSIAGSVEHMAMFPVDTLKTRIQALAGSST---LRQALSSILKSEGPAGLY 84
           L  WQ MIAGS+AGS ++M MFPV TL  R+   + S     +RQAL S++++EGP+ LY
Sbjct: 16  LQLWQLMIAGSVAGSFKNMTMFPVRTLDQRMLHRSYSQRHVGIRQALRSVIQTEGPSALY 75

Query: 85  RGIGAMGLGA-GPAHAVYFSVYEFCKQRFSIGNPNNPLAHAISGVCATVASDAALTPMDV 144
           RGI  M  GA GPA  V+FS Y+  K   S GNPNNP+ H IS     V S A  TP+D+
Sbjct: 76  RGIWYMRHGAMGPAQFVHFSFYDVSKNFLSTGNPNNPVVHVISWAFTAVWSYAVSTPVDM 135

Query: 145 VKQRLQLKSSPYKGVRDCARRILVEEGIGAF 172
            K R Q     YKGV DCA+R+  EEGI  F
Sbjct: 136 AKLRHQNGFGNYKGVWDCAKRVTHEEGISKF 166

The following BLAST results are available for this feature:

BLAST of Carg13298 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027417.1	1.2e-174	100.00	Mitoferrin, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6595407.1	4.5e-174	100.00	Mitoferrin, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022931904.1	2.2e-173	99.68	mitoferrin-like [Cucurbita moschata]	[more]
XP_023517443.1	4.6e-171	97.74	mitoferrin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022966611.1	6.3e-168	96.45	mitoferrin-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg13298 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55DY8	1.4e-56	42.42	Mitoferrin OS=Dictyostelium discoideum OX=44689 GN=mcfF PE=3 SV=1	[more]
O14281	8.5e-51	39.08	Uncharacterized mitochondrial carrier C8C9.12c OS=Schizosaccharomyces pombe (str...	[more]
P10566	7.2e-50	41.90	Mitochondrial RNA-splicing protein MRS3 OS=Saccharomyces cerevisiae (strain ATCC...	[more]
Q96A46	5.2e-48	38.26	Mitoferrin-2 OS=Homo sapiens OX=9606 GN=SLC25A28 PE=2 SV=1	[more]
Q8R0Z5	4.4e-47	38.44	Mitoferrin-2 OS=Mus musculus OX=10090 GN=Slc25a28 PE=2 SV=1	[more]

BLAST of Carg13298 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EVI8	1.1e-173	99.68	mitoferrin-like OS=Cucurbita moschata OX=3662 GN=LOC111438181 PE=3 SV=1	[more]
A0A6J1HPT3	3.0e-168	96.45	mitoferrin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466240 PE=3 SV=1	[more]
A0A6J1HNG7	3.3e-167	96.74	mitoferrin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466240 PE=3 SV=1	[more]
A0A0A0L006	2.3e-144	83.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G312860 PE=3 SV=1	[more]
A0A5A7T6D3	4.4e-143	82.69	Mitoferrin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G0019...	[more]

BLAST of Carg13298 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07030.1	3.6e-113	66.88	Mitochondrial substrate carrier family protein	[more]
AT2G30160.1	1.5e-111	65.29	Mitochondrial substrate carrier family protein	[more]
AT1G74240.1	6.3e-33	30.00	Mitochondrial substrate carrier family protein	[more]
AT1G34065.1	3.1e-32	31.82	S-adenosylmethionine carrier 2	[more]
AT1G07025.1	2.0e-31	50.33	Mitochondrial substrate carrier family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002067	Mitochondrial carrier protein	PRINTS	PR00926	MITOCARRIER	coord: 172..190 score: 25.19 coord: 130..148 score: 26.32 coord: 220..242 score: 27.3 coord: 30..43 score: 22.48 coord: 43..57 score: 28.42
IPR023395	Mitochondrial carrier domain superfamily	GENE3D	1.50.40.10	Mitochondrial carrier domain	coord: 7..177 e-value: 3.7E-44 score: 152.7
IPR023395	Mitochondrial carrier domain superfamily	GENE3D	1.50.40.10	Mitochondrial carrier domain	coord: 178..311 e-value: 6.1E-32 score: 112.5
IPR023395	Mitochondrial carrier domain superfamily	SUPERFAMILY	103506	Mitochondrial carrier	coord: 24..296
IPR018108	Mitochondrial substrate/solute carrier	PFAM	PF00153	Mito_carr	coord: 213..304 e-value: 2.1E-21 score: 75.7 coord: 25..109 e-value: 1.1E-20 score: 73.3 coord: 115..202 e-value: 2.2E-20 score: 72.3
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 211..301 score: 21.547621
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 25..108 score: 19.144875
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 115..199 score: 23.542759
None	No IPR available	PANTHER	PTHR45758:SF12	MITOFERRIN-LIKE	coord: 8..309
None	No IPR available	PANTHER	PTHR45758	MITOFERRIN-1-RELATED	coord: 8..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13298-RA	Carg13298-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048250	iron import into the mitochondrion
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005381	iron ion transmembrane transporter activity