Carg12461 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12461
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ammonium transporter
Location	Carg_Chr14: 203181 .. 205280 (+)
RNA-Seq Expression	Carg12461
Synteny	Carg12461

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGAAGATGGAAGTACTCCGCGGAGGCAGGAGGAGGAATATAGGGTCAAAACGGCACCCACTCGGCTCCAGTTGATAACATTTTTTATGCGACTCCGCTGAAAAAGAACCAACTACTTCCAATTTCTCACCCCATTCCTTCAACATCCTTCAAATCTCGCTATCAACACTTCAATTCCTAGAAACCCACTTGTCATACCCTCCAAATTTCCTTCCTCCATCAAATTTCAAACTTCATTCTCCAACCTCTCACTTTCTTCCAACCCTCAAACAATCACCAAATTCCCGATGAACAACACGGCCCAGCCGGGAAACATGGCCGCCGGATCCCCGCCATGGCTGAACACCGGCGACAACTCTTGGCAAATTACAGCCTCCACTCTCGTCGGACTTCAGAGCATGCCTGGCCTTGTGATTCTCTACGCCAGCATCGTCAAAAAGAAATGGGCTGTCAATTCTGCCTTTATGGTCCTCTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCCTCGTCGGTTACCGAATGGCCTTTGGCGACCAGCTGGTTCCTCTGTGGGGCAAAGGGATTCCAGCTCTGACCCAAAGTTACCTAATTGACCGAGCCAATATCCCCGAGAGTGGCAATACAAACAACGATGGAAGGGTAACGCTCAGAATTGAACCCAATATTCCGATGGCTTCGCTTGTGTATTTCCAGTTCACTTTTGCTGCGATTACGGTGATTTTACTGGCTGGCTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCGTTTGTTCCTCTTTGGGTAATTTTCGCATACACCGTCGGAGCATATTCTGTTTGGGGCGGAGGATTTCTCTTTCAATGGGGCGTAATTGATTACTCCGGCGGCTACGTGATCCATCTCTCTTCAGGGATTGCTGGCCTGACCGCCGCTTATTGGGTAAGAGCAAAAACCCGAGTGAATTTGATTTCGTTTTCGAACAGGGTTTTGAATTGATTGGTTTTCAACTGTTTGGTCAGGTTGGGCCGAGGATTAAGAGTGACAGGGAGAGGTTTCCGCCCAATAACGTACTGCTGATGCTGGCCGGAGCAGGGCTGCTGTGGATGGGATGGTCTGGATTTAACGGCGGGGCGCCGCACTCGGCGAACGTGGTCGCGTCGATAGCTGTGCTGAATACGAACGTGAGCGCCGCGACGAGCCTGCTGGTGTGGACCATTTTGGACGTATTGTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACTCCCGGCGCAGGTAATTTTTGTAATGTTTTTTTAGCTTACGTGTAATTATAATTAAATTAGGGGGTTTTAATAAATATAGAATTATTAATGTATATTACATTTGACTCAGATAATTATATTGTTTATAAAATTAAATTGTCAACATTTTTAACAAATAAATGGAAACAGGAATAGTGCAGACGTGGGCGGCTATGATAATGGGGATTCTTGGAGGGAGTATTCCATGGGTATCCATGATGGTCCTTCACAAAAAATTGAGTTTCTTGCAAAAGGTAAAAAACCAAATTTCGATCATTGTTGTTATGATTTACATAATTACATATTTAACTCTATTAATTAAAGTTCATTTATTTTATTTTAAATTTTGTCAAATCACGTGCAACAGGCTGACGACACGCTCGGCGTATTTCACACGCACGCGGTGGCGGGAATGATGGGCGGGCTTTTAACAGGGCTTTTAGCCGAGCCCACTCTATGCGATCTATACCTACCGATAACCGGCACACGCGGAGCCTTTTACGGCAAAAACGGCGGCGTTCAATTCCTGAAACAACTGGCCGGATCCGCGTTTATTATAGGATGGAACGTAGTGTCTACGACCATAATACTCCTCTTTATTCGGCTGTTTATGCCATTGAGGATGCCTGACGAGGAACTTATGATCGGCGATGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCCAGAAGGCACGGTACTGCGGGGAACAATGTGGAAGAAGGAAGGGCCTCGCCCTATATCCATGGTGCTAGAGGAGTAATCATCGAGTTG

mRNA sequence

TGGGAAGATGGAAGTACTCCGCGGAGGCAGGAGGAGGAATATAGGGTCAAAACGGCACCCACTCGGCTCCAAACCAACTACTTCCAATTTCTCACCCCATTCCTTCAACATCCTTCAAATCTCGCTATCAACACTTCAATTCCTAGAAACCCACTTGTCATACCCTCCAAATTTCCTTCCTCCATCAAATTTCAAACTTCATTCTCCAACCTCTCACTTTCTTCCAACCCTCAAACAATCACCAAATTCCCGATGAACAACACGGCCCAGCCGGGAAACATGGCCGCCGGATCCCCGCCATGGCTGAACACCGGCGACAACTCTTGGCAAATTACAGCCTCCACTCTCGTCGGACTTCAGAGCATGCCTGGCCTTGTGATTCTCTACGCCAGCATCGTCAAAAAGAAATGGGCTGTCAATTCTGCCTTTATGGTCCTCTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCCTCGTCGGTTACCGAATGGCCTTTGGCGACCAGCTGGTTCCTCTGTGGGGCAAAGGGATTCCAGCTCTGACCCAAAGTTACCTAATTGACCGAGCCAATATCCCCGAGAGTGGCAATACAAACAACGATGGAAGGGTAACGCTCAGAATTGAACCCAATATTCCGATGGCTTCGCTTGTGTATTTCCAGTTCACTTTTGCTGCGATTACGGTGATTTTACTGGCTGGCTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCGTTTGTTCCTCTTTGGGTAATTTTCGCATACACCGTCGGAGCATATTCTGTTTGGGGCGGAGGATTTCTCTTTCAATGGGGCGTAATTGATTACTCCGGCGGCTACGTGATCCATCTCTCTTCAGGGATTGCTGGCCTGACCGCCGCTTATTGGGTTGGGCCGAGGATTAAGAGTGACAGGGAGAGGTTTCCGCCCAATAACGTACTGCTGATGCTGGCCGGAGCAGGGCTGCTGTGGATGGGATGGTCTGGATTTAACGGCGGGGCGCCGCACTCGGCGAACGTGGTCGCGTCGATAGCTGTGCTGAATACGAACGTGAGCGCCGCGACGAGCCTGCTGGTGTGGACCATTTTGGACGTATTGTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACTCCCGGCGCAGGAATAGTGCAGACGTGGGCGGCTATGATAATGGGGATTCTTGGAGGGAGTATTCCATGGGTATCCATGATGGTCCTTCACAAAAAATTGAGTTTCTTGCAAAAGGCTGACGACACGCTCGGCGTATTTCACACGCACGCGGTGGCGGGAATGATGGGCGGGCTTTTAACAGGGCTTTTAGCCGAGCCCACTCTATGCGATCTATACCTACCGATAACCGGCACACGCGGAGCCTTTTACGGCAAAAACGGCGGCGTTCAATTCCTGAAACAACTGGCCGGATCCGCGTTTATTATAGGATGGAACGTAGTGTCTACGACCATAATACTCCTCTTTATTCGGCTGTTTATGCCATTGAGGATGCCTGACGAGGAACTTATGATCGGCGATGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCCAGAAGGCACGGTACTGCGGGGAACAATGTGGAAGAAGGAAGGGCCTCGCCCTATATCCATGGTGCTAGAGGAGTAATCATCGAGTTG

Coding sequence (CDS)

TGGGAAGATGGAAGTACTCCGCGGAGGCAGGAGGAGGAATATAGGGTCAAAACGGCACCCACTCGGCTCCAAACCAACTACTTCCAATTTCTCACCCCATTCCTTCAACATCCTTCAAATCTCGCTATCAACACTTCAATTCCTAGAAACCCACTTGTCATACCCTCCAAATTTCCTTCCTCCATCAAATTTCAAACTTCATTCTCCAACCTCTCACTTTCTTCCAACCCTCAAACAATCACCAAATTCCCGATGAACAACACGGCCCAGCCGGGAAACATGGCCGCCGGATCCCCGCCATGGCTGAACACCGGCGACAACTCTTGGCAAATTACAGCCTCCACTCTCGTCGGACTTCAGAGCATGCCTGGCCTTGTGATTCTCTACGCCAGCATCGTCAAAAAGAAATGGGCTGTCAATTCTGCCTTTATGGTCCTCTACGCCTTCGCCGCCGTTCTAATTTGTTGGGTCCTCGTCGGTTACCGAATGGCCTTTGGCGACCAGCTGGTTCCTCTGTGGGGCAAAGGGATTCCAGCTCTGACCCAAAGTTACCTAATTGACCGAGCCAATATCCCCGAGAGTGGCAATACAAACAACGATGGAAGGGTAACGCTCAGAATTGAACCCAATATTCCGATGGCTTCGCTTGTGTATTTCCAGTTCACTTTTGCTGCGATTACGGTGATTTTACTGGCTGGCTCTGTTCTTGCAAGAATGAACATCAAAGCTTGGATGGCGTTTGTTCCTCTTTGGGTAATTTTCGCATACACCGTCGGAGCATATTCTGTTTGGGGCGGAGGATTTCTCTTTCAATGGGGCGTAATTGATTACTCCGGCGGCTACGTGATCCATCTCTCTTCAGGGATTGCTGGCCTGACCGCCGCTTATTGGGTTGGGCCGAGGATTAAGAGTGACAGGGAGAGGTTTCCGCCCAATAACGTACTGCTGATGCTGGCCGGAGCAGGGCTGCTGTGGATGGGATGGTCTGGATTTAACGGCGGGGCGCCGCACTCGGCGAACGTGGTCGCGTCGATAGCTGTGCTGAATACGAACGTGAGCGCCGCGACGAGCCTGCTGGTGTGGACCATTTTGGACGTATTGTACTTTGGGAAGCCATCGGTGATTGGAGCCATTCAGGGTATGATGACTGGCTTGGCTTGCGTTACTCCCGGCGCAGGAATAGTGCAGACGTGGGCGGCTATGATAATGGGGATTCTTGGAGGGAGTATTCCATGGGTATCCATGATGGTCCTTCACAAAAAATTGAGTTTCTTGCAAAAGGCTGACGACACGCTCGGCGTATTTCACACGCACGCGGTGGCGGGAATGATGGGCGGGCTTTTAACAGGGCTTTTAGCCGAGCCCACTCTATGCGATCTATACCTACCGATAACCGGCACACGCGGAGCCTTTTACGGCAAAAACGGCGGCGTTCAATTCCTGAAACAACTGGCCGGATCCGCGTTTATTATAGGATGGAACGTAGTGTCTACGACCATAATACTCCTCTTTATTCGGCTGTTTATGCCATTGAGGATGCCTGACGAGGAACTTATGATCGGCGATGACGCCGTCCACGGCGAGGAGGCTTATGCTCTTTGGGGCGACGGCGAGAAATTCGATCCCAGAAGGCACGGTACTGCGGGGAACAATGTGGAAGAAGGAAGGGCCTCGCCCTATATCCATGGTGCTAGAGGAGTAATCATCGAGTTG

Protein sequence

WEDGSTPRRQEEEYRVKTAPTRLQTNYFQFLTPFLQHPSNLAINTSIPRNPLVIPSKFPSSIKFQTSFSNLSLSSNPQTITKFPMNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL

Homology

[Close]

BLAST of Carg12461 vs. NCBI nr
Match: KAG7017209.1 (Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0


Query: 1   WEDGSTPRRQEEEYRVKTAPTRLQTNYFQFLTPFLQHPSNLAINTSIPRNPLVIPSKFPS 60
           WEDGSTPRRQEEEYRVKTAPTRLQTNYFQFLTPFLQHPSNLAINTSIPRNPLVIPSKFPS
Sbjct: 1   WEDGSTPRRQEEEYRVKTAPTRLQTNYFQFLTPFLQHPSNLAINTSIPRNPLVIPSKFPS 60

Query: 61  SIKFQTSFSNLSLSSNPQTITKFPMNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQ 120
           SIKFQTSFSNLSLSSNPQTITKFPMNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQ
Sbjct: 61  SIKFQTSFSNLSLSSNPQTITKFPMNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQ 120

Query: 121 SMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPAL 180
           SMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPAL
Sbjct: 121 SMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPAL 180

Query: 181 TQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMN 240
           TQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMN
Sbjct: 181 TQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMN 240

Query: 241 IKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGP 300
           IKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGP
Sbjct: 241 IKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGP 300

Query: 301 RIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLV 360
           RIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLV
Sbjct: 301 RIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLV 360

Query: 361 WTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHK 420
           WTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHK
Sbjct: 361 WTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHK 420

Query: 421 KLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQF 480
           KLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQF
Sbjct: 421 KLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQF 480

Query: 481 LKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKF 540
           LKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKF
Sbjct: 481 LKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKF 540

Query: 541 DPRRHGTAGNNVEEGRASPYIHGARGVIIEL 572
           DPRRHGTAGNNVEEGRASPYIHGARGVIIEL
Sbjct: 541 DPRRHGTAGNNVEEGRASPYIHGARGVIIEL 571

[Close]

BLAST of Carg12461 vs. NCBI nr
Match: XP_022934329.1 (ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 974.5 bits (2518), Expect = 3.8e-280
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM
Sbjct: 301 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 360

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA
Sbjct: 421 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 480

Query: 565 RGVIIEL 572
           RGVIIEL
Sbjct: 481 RGVIIEL 487

[Close]

BLAST of Carg12461 vs. NCBI nr
Match: XP_023529182.1 (ammonium transporter 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 966.8 bits (2498), Expect = 8.0e-278
Identity = 482/487 (98.97%), Postives = 484/487 (99.38%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAG+VQTWAAMIMGILGGSIPWVSMMVLHKK SFLQKADDTLGVFHTHAVAGMM
Sbjct: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN+EEG ASPYIHGA
Sbjct: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480

Query: 565 RGVIIEL 572
           RGVIIEL
Sbjct: 481 RGVIIEL 487

[Close]

BLAST of Carg12461 vs. NCBI nr
Match: XP_022982616.1 (ammonium transporter 2-like [Cucurbita maxima])

HSP 1 Score: 965.7 bits (2495), Expect = 1.8e-277
Identity = 480/487 (98.56%), Postives = 485/487 (99.59%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRA+IPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRVT 120

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAG+VQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAG+M
Sbjct: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGLM 360

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWN+VSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNIVSTTIILLF 420

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN+EEG ASPYIHGA
Sbjct: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480

Query: 565 RGVIIEL 572
           RGVIIEL
Sbjct: 481 RGVIIEL 487

[Close]

BLAST of Carg12461 vs. NCBI nr
Match: KAG7027883.1 (Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907.1 bits (2343), Expect = 7.5e-260
Identity = 446/487 (91.58%), Postives = 469/487 (96.30%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTA P +MA G+PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 9   MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 68

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL QSYLIDRANIPESG+ ++DGRVT
Sbjct: 69  ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRVT 128

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
            RIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++W
Sbjct: 129 PRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTIW 188

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGG+VIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+L
Sbjct: 189 GGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGML 248

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTG
Sbjct: 249 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTG 308

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAG+VQTWAA+IMG++GGSIPWVSMM+LHKKLSFLQKADDTLGVFHTHAVAGMM
Sbjct: 309 LACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQKADDTLGVFHTHAVAGMM 368

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLC+LYLPI  +RGAFYGKNGGVQFLKQLAG+ FIIGWN+VSTTIILLF
Sbjct: 369 GGLLTGLLAEPTLCELYLPIPDSRGAFYGKNGGVQFLKQLAGAMFIIGWNIVSTTIILLF 428

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  NVEEG + PYI+GA
Sbjct: 429 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANVEEGTSPPYINGA 488

Query: 565 RGVIIEL 572
           RGV I L
Sbjct: 489 RGVTITL 495

[Close]

BLAST of Carg12461 vs. ExPASy Swiss-Prot
Match: Q9M6N7 (Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2)

HSP 1 Score: 709.1 bits (1829), Expect = 3.9e-203
Identity = 349/473 (73.78%), Postives = 397/473 (83.93%), Query Frame = 0


Query: 99  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 158
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL+CWVL
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 159 VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY 218
           + Y+MAFG++L+P WGKG PA  Q YL  +A IP S              P  PMA+LVY
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVA----------APYFPMATLVY 132

Query: 219 FQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYS 278
           FQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IF+YTVGAYS+WGGGFL+QWGVIDYS
Sbjct: 133 FQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYS 192

Query: 279 GGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHS 338
           GGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++
Sbjct: 193 GGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYA 252

Query: 339 ANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTW 398
           AN+ +SIAVLNTN+SAATSLLVWT LDV++FGKPSVIGAIQGM+TGLA VTPGAG++QTW
Sbjct: 253 ANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTW 312

Query: 399 AAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLC 458
           AA+I+G++ G+ PW SMM++HKK + LQK DDTL VF+THAVAG++GG++TGL A P LC
Sbjct: 313 AAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLC 372

Query: 459 DLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEEL 518
            L LP+  TRGAFYG NGG Q LKQLAG+AFI  WNVVSTTIILL IR+F+PLRM +EEL
Sbjct: 373 VLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEEL 432

Query: 519 MIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 572
            IGDDA HGEEAYALWGDGEKFD  RH       +E     Y+HGARGV I L
Sbjct: 433 GIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 475

[Close]

BLAST of Carg12461 vs. ExPASy Swiss-Prot
Match: Q84KJ7 (Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-1 PE=2 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 4.1e-192
Identity = 335/479 (69.94%), Postives = 393/479 (82.05%), Query Frame = 0


Query: 99  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 158
           P WLN GDN+WQ+TASTLVG+QSMPGLV+LY SIVKKKWAVNSAFM LYA+A+ L+ WVL
Sbjct: 15  PDWLNKGDNAWQLTASTLVGIQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASSLLVWVL 74

Query: 159 VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY 218
           VG+RMAFGDQL+P WGK   ALTQSYL+ RA +P + +    G +  R EP  P A+LV 
Sbjct: 75  VGFRMAFGDQLLPFWGKAGVALTQSYLVGRATLPATAH----GAIP-RTEPFYPEATLVL 134

Query: 219 FQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYS 278
           FQF FAAIT++LLAGSVL RMNIKAWMAF PLW++ +YTVGA+S+WGGGFL++WGVIDYS
Sbjct: 135 FQFEFAAITLVLLAGSVLGRMNIKAWMAFTPLWLLLSYTVGAFSLWGGGFLYRWGVIDYS 194

Query: 279 GGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHS 338
           GGYVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLWMGW+GFNGGAP++
Sbjct: 195 GGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWMGWAGFNGGAPYA 254

Query: 339 ANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTW 398
           AN+ AS+AVLNTNV AATSLL+WT LDV++F KPSVIGA+QGMMTGL C+TPGAG+VQTW
Sbjct: 255 ANIAASVAVLNTNVCAATSLLMWTCLDVIFFRKPSVIGAVQGMMTGLVCITPGAGLVQTW 314

Query: 399 AAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLC 458
           AA++MGI  GS+PW +MM+LHKK + L K DDTL VFHTHAVAG++GG+LTGLLA P L 
Sbjct: 315 AAVVMGIFAGSVPWFTMMILHKKSALLMKVDDTLAVFHTHAVAGLLGGILTGLLATPELF 374

Query: 459 DLYLPITGTRGAFYGKNGGV-QFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEE 518
            L   + G RGAFYG  GG+ Q  KQL G+AF+I WN+V TT ILL I LF+PLRMPDE+
Sbjct: 375 SLESTVPGLRGAFYG--GGIKQIGKQLGGAAFVIAWNLVVTTAILLGIGLFIPLRMPDEQ 434

Query: 519 LMIGDDAVHGEEAYALWGDGEKFDPRRHGTA---GNNVEEGRASPYIH--GARGVIIEL 572
           LMIGDDA HGEEAYALWGDGEKFD  RH  +   G    +G A   +   GARGV I+L
Sbjct: 435 LMIGDDAAHGEEAYALWGDGEKFDATRHDLSRGGGGGDRDGPAGERLSALGARGVTIQL 486

[Close]

BLAST of Carg12461 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.1e-188
Identity = 322/476 (67.65%), Postives = 388/476 (81.51%), Query Frame = 0


Query: 83  FPMNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSA 142
           F M+   QP  MA   P WLN GDN+WQ+ ++TLVG+QS+PGLVILY SIVKKKWAVNSA
Sbjct: 6   FNMSVAYQPSGMAV--PEWLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSA 65

Query: 143 FMVLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPES-----GNT 202
           FM LYAFAAV +CWV  GY M+FG +L+P WGK  PAL QS+L+ +A +P++     G  
Sbjct: 66  FMALYAFAAVWLCWVTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGG 125

Query: 203 NNDGRV-TLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAY 262
             D  V T  + P  PMA++VYFQ  FAAIT+ILLAGS+L RMNIKAWM FVPLW+ F+Y
Sbjct: 126 GADAVVETPWVNPLYPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSY 185

Query: 263 TVGAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLL 322
           TVGA+S+WGGGFLF WGV+DYSGGYVIHLSSG+AG TAAYWVGPR   DRERFPPNNVLL
Sbjct: 186 TVGAFSLWGGGFLFHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLL 245

Query: 323 MLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIG 382
           ML GAG+LWMGW+GFNGG P+SAN+ +S+AVLNTN+ AATSLLVWT LDV++F KPSVIG
Sbjct: 246 MLTGAGILWMGWAGFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIG 305

Query: 383 AIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFH 442
           A+QGM+TGL C+TPGAG+VQ WAA++MGIL GSIPW +MMV+HK+   LQ+ DDTLGVFH
Sbjct: 306 AVQGMITGLVCITPGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFH 365

Query: 443 THAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFY-GKNGGVQFLKQLAGSAFIIGWNV 502
           THAVAG +GG  TGL AEP LC L+LP+T +RGAFY G+ GG+QF++Q+AG+ FII WNV
Sbjct: 366 THAVAGFLGGATTGLFAEPVLCSLFLPVTNSRGAFYPGRGGGLQFVRQVAGALFIICWNV 425

Query: 503 VSTTIILLFIRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN 552
           V T+++ L +R  +PLRMP+EEL IGDDAVHGEEAYALWGDGEK+D  +HG   +N
Sbjct: 426 VVTSLVCLAVRAVVPLRMPEEELAIGDDAVHGEEAYALWGDGEKYDSTKHGWYSDN 479

[Close]

BLAST of Carg12461 vs. ExPASy Swiss-Prot
Match: Q8S233 (Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-3 PE=2 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 4.7e-188
Identity = 325/481 (67.57%), Postives = 388/481 (80.67%), Query Frame = 0


Query: 99  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 158
           P WLNTGDN WQ+ A+T VGLQSMPGLV+LY SIVKKKWAVNSAFM LYA+A+ LI WVL
Sbjct: 18  PEWLNTGDNGWQLAAATFVGLQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASTLIVWVL 77

Query: 159 VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRV-TLRIEPNIPMASLV 218
           VG+RMAFGD+L+P WGK   ALT+ +L+ RA++P + +   DG + + R EP  P AS+V
Sbjct: 78  VGFRMAFGDRLLPFWGKAGAALTEGFLVARASVPATAHYGKDGALESPRTEPFYPEASMV 137

Query: 219 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDY 278
            FQF  AAIT++LLAGS+L RMNIKAWMAF PLW++F+YTV A+S+WGGGFL+QWGVIDY
Sbjct: 138 LFQFELAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVCAFSLWGGGFLYQWGVIDY 197

Query: 279 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 338
           SGGYVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 198 SGGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 257

Query: 339 SANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQT 398
           + N+ ASIAVLNTNVSAA SLL WT LDV++FGKPSVIGA+QGMMTGL C+TPGAG+V T
Sbjct: 258 APNITASIAVLNTNVSAAASLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 317

Query: 399 WAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTL 458
           WAA++MGI GGS+PW SMM+LHK+ + LQK DDTL VFHTHAVAG++GG LTGL A P L
Sbjct: 318 WAAILMGICGGSLPWFSMMILHKRSALLQKVDDTLAVFHTHAVAGLLGGFLTGLFALPDL 377

Query: 459 CDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEE 518
             ++  I G RGAFYG  G  Q  KQ+AG+ F++ WNVV+TT+ILL + L +PLRMPDE+
Sbjct: 378 TAVHTHIPGARGAFYG-GGIAQVGKQIAGALFVVVWNVVATTVILLGVGLVVPLRMPDEQ 437

Query: 519 LMIGDDAVHGEEAYALWGDGEKFDPRRH-----GTAGNNVEEGRASPYI--HGARGVIIE 572
           L IGDDA HGEEAYALWGDGE+FD  RH     G  G  V +      +   GARGV I+
Sbjct: 438 LKIGDDAAHGEEAYALWGDGERFDVTRHEGARGGAWGAAVVDEAMDHRLAGMGARGVTIQ 497

[Close]

BLAST of Carg12461 vs. ExPASy Swiss-Prot
Match: Q8S230 (Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-2 PE=2 SV=2)

HSP 1 Score: 654.1 bits (1686), Expect = 1.5e-186
Identity = 321/481 (66.74%), Postives = 384/481 (79.83%), Query Frame = 0


Query: 99  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 158
           P WLN GD +WQ+ A+T VG+QSMPGLV++Y SIVKKKWAVNSAFM LYA+A+ LI WVL
Sbjct: 22  PAWLNKGDTAWQLVAATFVGIQSMPGLVVIYGSIVKKKWAVNSAFMALYAYASTLIVWVL 81

Query: 159 VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRV-TLRIEPNIPMASLV 218
           VG+RMAFGD+L+P W K  PALTQ +L+ RA  P + +  +DG + T R EP    A+LV
Sbjct: 82  VGFRMAFGDRLLPFWAKAGPALTQDFLVQRAVFPATAHYGSDGTLETPRTEPFYAEAALV 141

Query: 219 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDY 278
            F+F FAAIT++LLAGS+L RMNIKAWMAF PLW++F+YTVGA+S+WGGGFL+QWGVIDY
Sbjct: 142 LFEFEFAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVGAFSLWGGGFLYQWGVIDY 201

Query: 279 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 338
           SGGYVIHLSSG+AG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 202 SGGYVIHLSSGVAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 261

Query: 339 SANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQT 398
           + NV A++AVLNTNVSAATSLL WT LDV++FGKPSVIGA+QGMMTGL C+TPGAG+V T
Sbjct: 262 APNVTATVAVLNTNVSAATSLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 321

Query: 399 WAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTL 458
           W+AM+MG+  GS+PW +MM+LHKK +FL K DDTL VFHTHAVAG++GG+LTGLLA P L
Sbjct: 322 WSAMLMGMFAGSVPWFTMMILHKKSTFLMKVDDTLAVFHTHAVAGILGGVLTGLLATPEL 381

Query: 459 CDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEE 518
           C L  PI   RG FYG   G Q  KQL G+ F+  WN++ T+ ILL I LF+PLRM D++
Sbjct: 382 CALDCPIPNMRGVFYGSGIG-QLGKQLGGALFVTVWNLIVTSAILLCIGLFIPLRMSDDQ 441

Query: 519 LMIGDDAVHGEEAYALWGDGEKFD-----PRRHGTAGNNVEEGRASPYI--HGARGVIIE 572
           LMIGDDA HGEEAYALWGDGEKFD       R G AG    E      +   GARGV I+
Sbjct: 442 LMIGDDAAHGEEAYALWGDGEKFDVTRPETTRTGGAGGAGREDTMEQRLTNMGARGVTIQ 501

[Close]

BLAST of Carg12461 vs. ExPASy TrEMBL
Match: A0A6J1F297 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.9e-280
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM
Sbjct: 301 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 360

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA
Sbjct: 421 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 480

Query: 565 RGVIIEL 572
           RGVIIEL
Sbjct: 481 RGVIIEL 487

[Close]

BLAST of Carg12461 vs. ExPASy TrEMBL
Match: A0A6J1IZU0 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 8.6e-278
Identity = 480/487 (98.56%), Postives = 485/487 (99.59%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRA+IPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRVT 120

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAG+VQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAG+M
Sbjct: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGLM 360

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWN+VSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNIVSTTIILLF 420

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN+EEG ASPYIHGA
Sbjct: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480

Query: 565 RGVIIEL 572
           RGVIIEL
Sbjct: 481 RGVIIEL 487

[Close]

BLAST of Carg12461 vs. ExPASy TrEMBL
Match: A0A6J1GGC9 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111453662 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.4e-259
Identity = 444/487 (91.17%), Postives = 468/487 (96.10%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTA P +MA G+PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL QSYLIDRANIPESG+ ++DGRVT
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRVT 165

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
            RIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++W
Sbjct: 166 PRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTIW 225

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGG+VIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+L
Sbjct: 226 GGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGML 285

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTG
Sbjct: 286 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTG 345

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAG+VQTWAA+IMG++GGSIPWVSMM+LHK+LSFLQKADDTLGVFHTHAVAGMM
Sbjct: 346 LACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKRLSFLQKADDTLGVFHTHAVAGMM 405

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLC+LYLPI  +RGAFYGKNGGVQFLKQL G+ FIIGWN+VSTTIILLF
Sbjct: 406 GGLLTGLLAEPTLCELYLPIPDSRGAFYGKNGGVQFLKQLTGAMFIIGWNIVSTTIILLF 465

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  NVEEG + PYI+GA
Sbjct: 466 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANVEEGTSPPYINGA 525

Query: 565 RGVIIEL 572
           RGV I L
Sbjct: 526 RGVTITL 532

[Close]

BLAST of Carg12461 vs. ExPASy TrEMBL
Match: A0A6J1I2C9 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111470255 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.8e-259
Identity = 444/487 (91.17%), Postives = 467/487 (95.89%), Query Frame = 0


Query: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144
           MNNTA P +MA G+PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVHMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL QSYLIDRANIPESG+ ++DGRVT
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQSYLIDRANIPESGHVSHDGRVT 165

Query: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264
            RIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVG Y++W
Sbjct: 166 PRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGTYTIW 225

Query: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324
           GGGFLFQWGVIDYSGG+VIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+L
Sbjct: 226 GGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGML 285

Query: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTG
Sbjct: 286 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTG 345

Query: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444
           LACVTPGAG+VQTWAA+IMG++GGSIPWVSMM+LHKKLSFLQK DDTLGVFHTHAVAGMM
Sbjct: 346 LACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQKVDDTLGVFHTHAVAGMM 405

Query: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504
           GGLLTGLLAEPTLC+LYLPI  +RGAFYGKNGGVQFLKQLAG+ FIIGWN+VSTTIILLF
Sbjct: 406 GGLLTGLLAEPTLCELYLPIPDSRGAFYGKNGGVQFLKQLAGAMFIIGWNIVSTTIILLF 465

Query: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564
           IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  NVEEG + PYI+GA
Sbjct: 466 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANVEEGTSPPYINGA 525

Query: 565 RGVIIEL 572
           RGV I L
Sbjct: 526 RGVTIPL 532

[Close]

BLAST of Carg12461 vs. ExPASy TrEMBL
Match: A0A0A0LCQ1 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 442/478 (92.47%), Postives = 458/478 (95.82%), Query Frame = 0


Query: 94  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 153
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 154 ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 213
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESG--VNDGRGTPRIQPNVPM 120

Query: 214 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 273
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180

Query: 274 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 333
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240

Query: 334 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 393
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 300

Query: 394 IVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLA 453
           +VQTWAA+IMGIL GSIPW+SMMVLHKKLSFLQKADDTLGVFHTHAVAG+MGGLLTGLLA
Sbjct: 301 VVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 360

Query: 454 EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRM 513
           EPTLCDLYLPI GTRGAFYG +GGVQFLKQLAGS FIIGWN+VSTT+ILLFIRLFMPLRM
Sbjct: 361 EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLAGSTFIIGWNIVSTTVILLFIRLFMPLRM 420

Query: 514 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 572
           PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEG  SPYI+GARGVIIEL
Sbjct: 421 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 476

[Close]

BLAST of Carg12461 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 709.1 bits (1829), Expect = 2.8e-204
Identity = 349/473 (73.78%), Postives = 397/473 (83.93%), Query Frame = 0


Query: 99  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 158
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL+CWVL
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 159 VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY 218
           + Y+MAFG++L+P WGKG PA  Q YL  +A IP S              P  PMA+LVY
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVA----------APYFPMATLVY 132

Query: 219 FQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYS 278
           FQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IF+YTVGAYS+WGGGFL+QWGVIDYS
Sbjct: 133 FQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYS 192

Query: 279 GGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHS 338
           GGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++
Sbjct: 193 GGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYA 252

Query: 339 ANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGIVQTW 398
           AN+ +SIAVLNTN+SAATSLLVWT LDV++FGKPSVIGAIQGM+TGLA VTPGAG++QTW
Sbjct: 253 ANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTW 312

Query: 399 AAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLC 458
           AA+I+G++ G+ PW SMM++HKK + LQK DDTL VF+THAVAG++GG++TGL A P LC
Sbjct: 313 AAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLC 372

Query: 459 DLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEEL 518
            L LP+  TRGAFYG NGG Q LKQLAG+AFI  WNVVSTTIILL IR+F+PLRM +EEL
Sbjct: 373 VLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEEL 432

Query: 519 MIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 572
            IGDDA HGEEAYALWGDGEKFD  RH       +E     Y+HGARGV I L
Sbjct: 433 GIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 475

[Close]

BLAST of Carg12461 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 564.3 bits (1453), Expect = 1.1e-160
Identity = 274/359 (76.32%), Postives = 312/359 (86.91%), Query Frame = 0


Query: 213 MASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQW 272
           MA+LVYFQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IF+YTVGAYS+WGGGFL+QW
Sbjct: 1   MATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQW 60

Query: 273 GVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 332
           GVIDYSGGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFN
Sbjct: 61  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 120

Query: 333 GGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGA 392
           GGAP++AN+ +SIAVLNTN+SAATSLLVWT LDV++FGKPSVIGAIQGM+TGLA VTPGA
Sbjct: 121 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 180

Query: 393 GIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMMGGLLTGLL 452
           G++QTWAA+I+G++ G+ PW SMM++HKK + LQK DDTL VF+THAVAG++GG++TGL 
Sbjct: 181 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 240

Query: 453 AEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLR 512
           A P LC L LP+  TRGAFYG NGG Q LKQLAG+AFI  WNVVSTTIILL IR+F+PLR
Sbjct: 241 AHPDLCVLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLR 300

Query: 513 MPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGARGVIIEL 572
           M +EEL IGDDA HGEEAYALWGDGEKFD  RH       +E     Y+HGARGV I L
Sbjct: 301 MAEEELGIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 359

[Close]

BLAST of Carg12461 vs. TAIR 10
Match: AT1G64780.1 (ammonium transporter 1;2 )

HSP 1 Score: 105.9 bits (263), Expect = 1.1e-22
Identity = 126/479 (26.30%), Postives = 191/479 (39.87%), Query Frame = 0


Query: 106 DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAF 165
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  I + L G+  AF
Sbjct: 52  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAF 111

Query: 166 GDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYFQFTFAA 225
           G         G      S+    ++ PE   ++                S   +Q+ FA 
Sbjct: 112 GTP-----SNGFIGRHHSFFA-LSSYPERPGSD---------------FSFFLYQWAFAI 171

Query: 226 ITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWG-----------GGFLFQWGV 285
               + +GS+  R    A++ +      F Y   ++  W               LF  G 
Sbjct: 172 AAAGITSGSIAERTQFVAYLIYSTFLTGFVYPTVSHWFWSSDGWASASRSDNNLLFGSGA 231

Query: 286 IDYSGGYVIHLSSGIAGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLWMGWS 345
           ID++G  V+H+  GIAGL  A   GPRI + DR         ++  L++ G  LLW GW 
Sbjct: 232 IDFAGSGVVHMVGGIAGLCGALVEGPRIGRFDRSGRSVALRGHSASLVVLGTFLLWFGWY 291

Query: 346 GFNGGA------------PHSA--NVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVI 405
           GFN G+            P+    + V   AV  T +S  T+ L       L  G  +VI
Sbjct: 292 GFNPGSFLTILKGYDKSRPYYGQWSAVGRTAV-TTTLSGCTAALTTLFSKRLLAGHWNVI 351

Query: 406 GAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVF 465
               G++ G A +T G  +V+ WAA++ G +   +  +   +L KKL    K DD L   
Sbjct: 352 DVCNGLLGGFAAITSGCAVVEPWAAIVCGFVASWV-LIGFNLLAKKL----KYDDPLEAA 411

Query: 466 HTHAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNV 525
             H   G  G + TGL A     +         G F G  GG     Q+     I+GW  
Sbjct: 412 QLHGGCGAWGLIFTGLFARKEYVNEIYSGDRPYGLFMG-GGGKLLAAQIVQIIVIVGWVT 471

Query: 526 VSTTIILLFIRLFMPLRMPDEELMIGDDAV-HGEEAYALWGDGEKFDPRRHGTAGNNVE 554
           V+   +   +     LR+  E+ M G D   HG  AYA + D +    +  G     VE
Sbjct: 472 VTMGPLFYGLHKMNLLRISAEDEMAGMDMTRHGGFAYA-YNDEDDVSTKPWGHFAGRVE 500

[Close]

BLAST of Carg12461 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 105.1 bits (261), Expect = 1.8e-22
Identity = 121/466 (25.97%), Postives = 187/466 (40.13%), Query Frame = 0


Query: 106 DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAF 165
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  + + L GY  AF
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 166 GDQLVPLWGK------GIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYF 225
           G+      G+        P LT  Y                              S   +
Sbjct: 106 GESSDGFIGRHNFGLQNFPTLTSDY------------------------------SFFLY 165

Query: 226 QFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWG-GGF---------L 285
           Q+ FA     + +GS+  R    A++ +      F Y V ++  W   G+         L
Sbjct: 166 QWAFAIAAAGITSGSIAERTKFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRL 225

Query: 286 FQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPP---------NNVLLMLAG 345
           F  G ID++G  V+H+  GIAGL  A   GPRI     RFP          ++  L++ G
Sbjct: 226 FGTGAIDFAGSGVVHMVGGIAGLWGALIEGPRI----GRFPDGGHAIALRGHSASLVVLG 285

Query: 346 AGLLWMGWSGFNGGA------PHSA-------NVVASIAVLNTNVSAATSLLVWTILDVL 405
             LLW GW GFN G+      P+++       + +   AV  T +S  T+ L       L
Sbjct: 286 TFLLWFGWYGFNPGSFTKILIPYNSGSNYGQWSGIGRTAV-TTTLSGCTAALTTLFGKRL 345

Query: 406 YFGKPSVIGAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQK 465
             G  +V     G++ G A +T G  +V  WAA++ G +   +     ++   KL+ L K
Sbjct: 346 LSGHWNVTDVCNGLLGGFAAITAGCSVVDPWAAIVCGFVASLV-----LIGCNKLAELLK 405

Query: 466 ADDTLGVFHTHAVAGMMGGLLTGLLA-EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAG 525
            DD L     H   G  G +  GL A E  + ++Y    G     +   GG     QL  
Sbjct: 406 YDDPLEAAQLHGGCGAWGLIFVGLFAKEKYINEVYGASPGRHYGLFMGGGGKLLGAQLVQ 465

Query: 526 SAFIIGWNVVSTTIILLFIRLFMPLRMPDEELMIGDD-AVHGEEAY 532
              I+GW   +   +   ++    LR+ ++  M G D A HG  AY
Sbjct: 466 IIVIVGWVSATMGTLFFILKKLNLLRISEQHEMRGMDLAGHGGFAY 470

[Close]

BLAST of Carg12461 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 104.8 bits (260), Expect = 2.4e-22
Identity = 128/488 (26.23%), Postives = 189/488 (38.73%), Query Frame = 0


Query: 106 DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAF 165
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  + + L GY  AF
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 166 GDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY----FQF 225
           G       GK    L                             +IP AS  Y    +Q+
Sbjct: 102 GSPSNGFIGKHYFGL----------------------------KDIPTASADYSNFLYQW 161

Query: 226 TFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWG-----------GGFLF 285
            FA     + +GS+  R    A++ +      F Y V ++  W            G  LF
Sbjct: 162 AFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLF 221

Query: 286 QWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLW 345
             G ID++G  V+H+  GIAGL  A   GPR+ + D          ++  L++ G  LLW
Sbjct: 222 STGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLW 281

Query: 346 MGWSGFNGGAPHSANVVASIAVLNTNVSA------ATSLLVWTILDVLYFGKP------S 405
            GW GFN G+ +   V       N   SA       T+L   T      FGK       +
Sbjct: 282 FGWYGFNPGSFNKILVTYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWN 341

Query: 406 VIGAIQGMMTGLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLG 465
           V     G++ G A +T G  +V+ WAA+I G +   +     ++   KL+   K DD L 
Sbjct: 342 VTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALV-----LLGCNKLAEKLKYDDPLE 401

Query: 466 VFHTHAVAGMMGGLLTGLLA-EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIG 525
               H   G  G + T L A E  L  +Y    G     +   GG     QL     I G
Sbjct: 402 AAQLHGGCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLLGAQLIQIIVITG 461

Query: 526 WNVVSTTIILLFIRLFMPLRMPDEELMIGDDAV-HGEEAYALWGDGE--------KFDPR 552
           W   +   +   ++    LR+  E+ M G D   HG  AY  + D E        + +PR
Sbjct: 462 WVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDESHKAIQLRRVEPR 495

The following BLAST results are available for this feature:

BLAST of Carg12461 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017209.1	0.0e+00	100.00	Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022934329.1	3.8e-280	100.00	ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transpor...	[more]
XP_023529182.1	8.0e-278	98.97	ammonium transporter 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982616.1	1.8e-277	98.56	ammonium transporter 2-like [Cucurbita maxima]	[more]
KAG7027883.1	7.5e-260	91.58	Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Carg12461 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M6N7	3.9e-203	73.78	Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2	[more]
Q84KJ7	4.1e-192	69.94	Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]
Q84KJ6	2.1e-188	67.65	Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3...	[more]
Q8S233	4.7e-188	67.57	Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]
Q8S230	1.5e-186	66.74	Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]

BLAST of Carg12461 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F297	1.9e-280	100.00	Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1	[more]
A0A6J1IZU0	8.6e-278	98.56	Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1	[more]
A0A6J1GGC9	2.4e-259	91.17	Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111453662 PE=3 SV=1	[more]
A0A6J1I2C9	6.8e-259	91.17	Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111470255 PE=3 SV=1	[more]
A0A0A0LCQ1	1.7e-254	92.47	Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1	[more]

BLAST of Carg12461 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38290.1	2.8e-204	73.78	ammonium transporter 2	[more]
AT2G38290.2	1.1e-160	76.32	ammonium transporter 2	[more]
AT1G64780.1	1.1e-22	26.30	ammonium transporter 1;2	[more]
AT3G24290.1	1.8e-22	25.97	ammonium transporter 1;5	[more]
AT4G13510.1	2.4e-22	26.23	ammonium transporter 1;1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002229	Blood group Rhesus C/E/D polypeptide	PRINTS	PR00342	RHESUSRHD	coord: 346..368 score: 29.23 coord: 401..419 score: 24.27 coord: 220..236 score: 33.99 coord: 315..333 score: 41.52
IPR029020	Ammonium/urea transporter	GENE3D	1.10.3430.10	Ammonium transporter AmtB like domains	coord: 79..534 e-value: 3.1E-119 score: 400.5
IPR024041	Ammonium transporter AmtB-like domain	PFAM	PF00909	Ammonium_transp	coord: 109..531 e-value: 2.1E-80 score: 270.4
IPR001905	Ammonium transporter	TIGRFAM	TIGR00836	TIGR00836	coord: 106..531 e-value: 2.9E-113 score: 376.8
None	No IPR available	PANTHER	PTHR43029:SF34	AMMONIUM TRANSPORTER 2	coord: 94..568
None	No IPR available	PANTHER	PTHR43029	AMMONIUM TRANSPORTER MEP2	coord: 94..568
None	No IPR available	SUPERFAMILY	111352	Ammonium transporter	coord: 101..513
IPR018047	Ammonium transporter, conserved site	PROSITE	PS01219	AMMONIUM_TRANSP	coord: 276..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12461-RA	Carg12461-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0072488	ammonium transmembrane transport
biological_process	GO:0019740	nitrogen utilization
biological_process	GO:0015696	ammonium transport
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015398	high-affinity secondary active ammonium transmembrane transporter activity
molecular_function	GO:0008519	ammonium transmembrane transporter activity