Carg10221 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10221
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein-S-isoprenylcysteine O-methyltransferase
Location	Carg_Chr17: 53686 .. 54234 (+)
RNA-Seq Expression	Carg10221
Synteny	Carg10221

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATATTTGCAATCATCTTCTTTCACAGTTCTGAATATATGTTAGCAGTTGGAATTCATGGGAAATCCAACGTAACTCTAAAGTCCCTTCTGATAAGCAAAAACTATCTGTTGGCAATGATGTTATGTTTCTTAGAGTACTTCGTTGAAATTGTGTTGTGTCCCGGTCTTAAGGAATACTGGTGGGTTAGTCAGACAGGCCTTGCAATGGTTGTAGTTGGAGAAACAGTACGTAAACTGGCGATCGTAACTGCAGGTCGGTCATTTACACATCTGATTAAGATCTATCATGAGGACCATCACAAGCTGATTACTTATGGAGTCTATAGATTTATTCGTCATCCTGGATACAGCGGTTTCCTTGTGTGGGCAGTTGGCACTCAAATCATGCTCTGTAATCCCATTTCAACAATTGGATTTGCTGTTGTGGTTTGGCAATTCTTTGCTGAACGAATTCCATATGAAGAGTACTTCCTGAGACAGTTTTTTGGTCGCGAGTATGAGGAGTACGCGAATCGGGTACCTTCTGGAGTGCCATTCGTGAAATGA

mRNA sequence

ATGATATTTGCAATCATCTTCTTTCACAGTTCTGAATATATGTTAGCAGTTGGAATTCATGGGAAATCCAACGTAACTCTAAAGTCCCTTCTGATAAGCAAAAACTATCTGTTGGCAATGATGTTATGTTTCTTAGAGTACTTCGTTGAAATTGTGTTGTGTCCCGGTCTTAAGGAATACTGGTGGGTTAGTCAGACAGGCCTTGCAATGGTTGTAGTTGGAGAAACAGTACGTAAACTGGCGATCGTAACTGCAGGTCGGTCATTTACACATCTGATTAAGATCTATCATGAGGACCATCACAAGCTGATTACTTATGGAGTCTATAGATTTATTCGTCATCCTGGATACAGCGGTTTCCTTGTGTGGGCAGTTGGCACTCAAATCATGCTCTGTAATCCCATTTCAACAATTGGATTTGCTGTTGTGGTTTGGCAATTCTTTGCTGAACGAATTCCATATGAAGAGTACTTCCTGAGACAGTTTTTTGGTCGCGAGTATGAGGAGTACGCGAATCGGGTACCTTCTGGAGTGCCATTCGTGAAATGA

Coding sequence (CDS)

ATGATATTTGCAATCATCTTCTTTCACAGTTCTGAATATATGTTAGCAGTTGGAATTCATGGGAAATCCAACGTAACTCTAAAGTCCCTTCTGATAAGCAAAAACTATCTGTTGGCAATGATGTTATGTTTCTTAGAGTACTTCGTTGAAATTGTGTTGTGTCCCGGTCTTAAGGAATACTGGTGGGTTAGTCAGACAGGCCTTGCAATGGTTGTAGTTGGAGAAACAGTACGTAAACTGGCGATCGTAACTGCAGGTCGGTCATTTACACATCTGATTAAGATCTATCATGAGGACCATCACAAGCTGATTACTTATGGAGTCTATAGATTTATTCGTCATCCTGGATACAGCGGTTTCCTTGTGTGGGCAGTTGGCACTCAAATCATGCTCTGTAATCCCATTTCAACAATTGGATTTGCTGTTGTGGTTTGGCAATTCTTTGCTGAACGAATTCCATATGAAGAGTACTTCCTGAGACAGTTTTTTGGTCGCGAGTATGAGGAGTACGCGAATCGGGTACCTTCTGGAGTGCCATTCGTGAAATGA

Protein sequence

MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEYWWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGFLVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPFVK

Homology

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BLAST of Carg10221 vs. NCBI nr
Match: KAG6574775.1 (Protein-S-isoprenylcysteine O-methyltransferase B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.4 bits (947), Expect = 1.8e-98
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY
Sbjct: 16  MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 76  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF
Sbjct: 136 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. NCBI nr
Match: KAG7013355.1 (Protein-S-isoprenylcysteine O-methyltransferase B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369.4 bits (947), Expect = 1.8e-98
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY
Sbjct: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF
Sbjct: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180

Query: 181 VK 183
           VK
Sbjct: 181 VK 182

[Close]

BLAST of Carg10221 vs. NCBI nr
Match: XP_023006693.1 (protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita maxima] >XP_023006694.1 protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita maxima])

HSP 1 Score: 367.9 bits (943), Expect = 5.2e-98
Identity = 181/182 (99.45%), Postives = 181/182 (99.45%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY
Sbjct: 16  MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 76  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGF VVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF
Sbjct: 136 LVWAVGTQIMLCNPISTIGFVVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. NCBI nr
Match: XP_022958895.1 (protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita moschata])

HSP 1 Score: 367.9 bits (943), Expect = 5.2e-98
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY
Sbjct: 16  MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 76  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYAN+VPSGVPF
Sbjct: 136 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANQVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. NCBI nr
Match: XP_023548051.1 (protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita pepo subsp. pepo] >XP_023548052.1 protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 364.0 bits (933), Expect = 7.5e-97
Identity = 179/182 (98.35%), Postives = 180/182 (98.90%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFV IVLCPGLKEY
Sbjct: 16  MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVGIVLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 76  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYE+Y NRVPSGVPF
Sbjct: 136 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEQYTNRVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. ExPASy Swiss-Prot
Match: Q93W54 (Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana OX=3702 GN=ICMTB PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 6.0e-73
Identity = 128/181 (70.72%), Postives = 154/181 (85.08%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           M  AIIFFH+SEY+LA+ IHG S VTL SLLISK+Y LAM++  LEY  EIV  PGLK++
Sbjct: 16  MFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLEYIAEIVFFPGLKQH 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WW+S  GL M+++GE +RK AI+TAGRSFTHLIKI  E+HHKL+T GVY+ +RHP YSGF
Sbjct: 76  WWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTEGVYQIMRHPSYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           L+W+VGTQ+MLCNPIS I FAVVVW+FFAERIPYEE++L+QFFGR+Y EYA RVPSGVPF
Sbjct: 136 LIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGRQYVEYAQRVPSGVPF 195

Query: 181 V 182
           V
Sbjct: 196 V 196

[Close]

BLAST of Carg10221 vs. ExPASy Swiss-Prot
Match: Q9FMW9 (Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana OX=3702 GN=ICMTA PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 7.4e-71
Identity = 124/181 (68.51%), Postives = 152/181 (83.98%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           M+ ++IFFH SEY+LA+ IHG SNVTL SLLI+K+Y LAM+L  LEY  EI+L PGLK++
Sbjct: 16  MLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLEYLTEIILFPGLKQH 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVS  GL M++VGE +RK AI+TAGRSFTHLIKI +E+HH L+T+GVYR +RHP Y GF
Sbjct: 76  WWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTHGVYRLMRHPSYCGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           L+W+VGTQ+MLCNP+S + FAVVVW+FFA+RIPYEEYFL QFFG +Y EYA  V SGVPF
Sbjct: 136 LIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGVQYLEYAESVASGVPF 195

Query: 181 V 182
           V
Sbjct: 196 V 196

[Close]

BLAST of Carg10221 vs. ExPASy Swiss-Prot
Match: A2XX73 (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=ICMT PE=3 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-69
Identity = 119/179 (66.48%), Postives = 151/179 (84.36%), Query Frame = 0


Query: 4   AIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEYWWV 63
           A++ FH SEY+LA   HG+ NVT  SLLISK Y+LAM    LE+  E +L P LKEYW+V
Sbjct: 13  ALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWFV 72

Query: 64  SQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGFLVW 123
           S  GL MV++GE +RKLA+VTAGRSFTH+I+I++ED HKLIT+GVYR +RHPGYSGFL+W
Sbjct: 73  SYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRHPGYSGFLIW 132

Query: 124 AVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPFVK 183
           AVGTQ+MLCNP+ST+ F +V+W+FF++RIPYEE+FLRQFFGREYEEYA +V SG+PF++
Sbjct: 133 AVGTQVMLCNPLSTVAFTLVLWRFFSKRIPYEEFFLRQFFGREYEEYAQKVHSGLPFIE 191

[Close]

BLAST of Carg10221 vs. ExPASy Swiss-Prot
Match: Q7XSR9 (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=ICMT PE=3 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-69
Identity = 119/179 (66.48%), Postives = 151/179 (84.36%), Query Frame = 0


Query: 4   AIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEYWWV 63
           A++ FH SEY+LA   HG+ NVT  SLLISK Y+LAM    LE+  E +L P LKEYW+V
Sbjct: 13  ALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWFV 72

Query: 64  SQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGFLVW 123
           S  GL MV++GE +RKLA+VTAGRSFTH+I+I++ED HKLIT+GVYR +RHPGYSGFL+W
Sbjct: 73  SYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRHPGYSGFLIW 132

Query: 124 AVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPFVK 183
           AVGTQ+MLCNP+ST+ F +V+W+FF++RIPYEE+FLRQFFGREYEEYA +V SG+PF++
Sbjct: 133 AVGTQVMLCNPLSTVAFTLVLWRFFSKRIPYEEFFLRQFFGREYEEYAQKVHSGLPFIE 191

[Close]

BLAST of Carg10221 vs. ExPASy Swiss-Prot
Match: O60725 (Protein-S-isoprenylcysteine O-methyltransferase OS=Homo sapiens OX=9606 GN=ICMT PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 4.4e-39
Identity = 81/181 (44.75%), Postives = 117/181 (64.64%), Query Frame = 0


Query: 4   AIIFFHSSEYMLAVGIHGKSNVTLKSLLI--SKNYLLAMMLCFLEYFVEIVLCPGLKEYW 63
           ++  FH SEY L   ++   +++L S L+  S  Y +A +  +LE+ +E +  P LK+  
Sbjct: 99  SLSLFHYSEY-LVTAVNNPKSLSLDSFLLNHSLEYTVAALSSWLEFTLENIFWPELKQIT 158

Query: 64  WVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGFL 123
           W+S TGL MVV GE +RK A+ TAG +F H+++    D H L+T GVY + RHP Y G+ 
Sbjct: 159 WLSVTGLLMVVFGECLRKAAMFTAGSNFNHVVQNEKSDTHTLVTSGVYAWFRHPSYVGWF 218

Query: 124 VWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPFV 183
            W++GTQ+MLCNPI  + +A+ VW+FF +R   EE  L  FFG EY EY  RVP+G+PF+
Sbjct: 219 YWSIGTQVMLCNPICGVSYALTVWRFFRDRTEEEEISLIHFFGEEYLEYKKRVPTGLPFI 278

[Close]

BLAST of Carg10221 vs. ExPASy TrEMBL
Match: A0A6J1L5N1 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499352 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.5e-98
Identity = 181/182 (99.45%), Postives = 181/182 (99.45%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY
Sbjct: 16  MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 76  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGF VVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF
Sbjct: 136 LVWAVGTQIMLCNPISTIGFVVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. ExPASy TrEMBL
Match: A0A6J1H4S0 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460045 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.5e-98
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY
Sbjct: 16  MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF
Sbjct: 76  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYAN+VPSGVPF
Sbjct: 136 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANQVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. ExPASy TrEMBL
Match: A0A6J1DSC7 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111023074 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 159/182 (87.36%), Postives = 173/182 (95.05%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFH SEY+LAVGIHG+SNVTL SLLISKNY+LAM+L FLEYF+EI+LCPGLKEY
Sbjct: 16  MIFAIIFFHISEYILAVGIHGRSNVTLNSLLISKNYILAMVLSFLEYFLEIMLCPGLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVV+GE VRKLAI+TAGRSFTHLIKIYHEDHHKL+TYGVYRF+RHPGYSGF
Sbjct: 76  WWVSQTGLAMVVIGEIVRKLAIITAGRSFTHLIKIYHEDHHKLVTYGVYRFVRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNP+ST+ FAVVVW FFAERIPYEEY+LR+FFG EY EYANRV SGVPF
Sbjct: 136 LVWAVGTQIMLCNPVSTVAFAVVVWHFFAERIPYEEYYLRRFFGHEYVEYANRVSSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. ExPASy TrEMBL
Match: A0A6J1DPQ1 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111023074 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 159/182 (87.36%), Postives = 173/182 (95.05%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAIIFFH SEY+LAVGIHG+SNVTL SLLISKNY+LAM+L FLEYF+EI+LCPGLKEY
Sbjct: 41  MIFAIIFFHISEYILAVGIHGRSNVTLNSLLISKNYILAMVLSFLEYFLEIMLCPGLKEY 100

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVSQTGLAMVV+GE VRKLAI+TAGRSFTHLIKIYHEDHHKL+TYGVYRF+RHPGYSGF
Sbjct: 101 WWVSQTGLAMVVIGEIVRKLAIITAGRSFTHLIKIYHEDHHKLVTYGVYRFVRHPGYSGF 160

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWAVGTQIMLCNP+ST+ FAVVVW FFAERIPYEEY+LR+FFG EY EYANRV SGVPF
Sbjct: 161 LVWAVGTQIMLCNPVSTVAFAVVVWHFFAERIPYEEYYLRRFFGHEYVEYANRVSSGVPF 220

Query: 181 VK 183
           VK
Sbjct: 221 VK 222

[Close]

BLAST of Carg10221 vs. ExPASy TrEMBL
Match: A0A6J1D2G4 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111016968 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.5e-87
Identity = 158/182 (86.81%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           MIFAII FH SEY+LAVGIHG+SNVTL SLLISKNYLLAM+L FLEYF+E +LCP LKEY
Sbjct: 16  MIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEYFLETLLCPSLKEY 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WW+SQ GLAMVV+GETVRKLAI+TAGRSFTHLIKIYHEDHHKL+T+GVYRF+RHPGYSGF
Sbjct: 76  WWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHGVYRFVRHPGYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           LVWA+GTQIMLCNPISTI FAVVVW FFA+RIPYEEYFLRQFFG EY+EYANRVPSGVPF
Sbjct: 136 LVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEYKEYANRVPSGVPF 195

Query: 181 VK 183
           VK
Sbjct: 196 VK 197

[Close]

BLAST of Carg10221 vs. TAIR 10
Match: AT5G08335.1 (Isoprenylcysteine carboxyl methyltransferase (ICMT) family )

HSP 1 Score: 275.0 bits (702), Expect = 4.3e-74
Identity = 128/181 (70.72%), Postives = 154/181 (85.08%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           M  AIIFFH+SEY+LA+ IHG S VTL SLLISK+Y LAM++  LEY  EIV  PGLK++
Sbjct: 16  MFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLEYIAEIVFFPGLKQH 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WW+S  GL M+++GE +RK AI+TAGRSFTHLIKI  E+HHKL+T GVY+ +RHP YSGF
Sbjct: 76  WWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTEGVYQIMRHPSYSGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           L+W+VGTQ+MLCNPIS I FAVVVW+FFAERIPYEE++L+QFFGR+Y EYA RVPSGVPF
Sbjct: 136 LIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGRQYVEYAQRVPSGVPF 195

Query: 181 V 182
           V
Sbjct: 196 V 196

[Close]

BLAST of Carg10221 vs. TAIR 10
Match: AT5G23320.1 (homolog of yeast STE14 A )

HSP 1 Score: 268.1 bits (684), Expect = 5.2e-72
Identity = 124/181 (68.51%), Postives = 152/181 (83.98%), Query Frame = 0


Query: 1   MIFAIIFFHSSEYMLAVGIHGKSNVTLKSLLISKNYLLAMMLCFLEYFVEIVLCPGLKEY 60
           M+ ++IFFH SEY+LA+ IHG SNVTL SLLI+K+Y LAM+L  LEY  EI+L PGLK++
Sbjct: 16  MLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLEYLTEIILFPGLKQH 75

Query: 61  WWVSQTGLAMVVVGETVRKLAIVTAGRSFTHLIKIYHEDHHKLITYGVYRFIRHPGYSGF 120
           WWVS  GL M++VGE +RK AI+TAGRSFTHLIKI +E+HH L+T+GVYR +RHP Y GF
Sbjct: 76  WWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTHGVYRLMRHPSYCGF 135

Query: 121 LVWAVGTQIMLCNPISTIGFAVVVWQFFAERIPYEEYFLRQFFGREYEEYANRVPSGVPF 180
           L+W+VGTQ+MLCNP+S + FAVVVW+FFA+RIPYEEYFL QFFG +Y EYA  V SGVPF
Sbjct: 136 LIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGVQYLEYAESVASGVPF 195

Query: 181 V 182
           V
Sbjct: 196 V 196

The following BLAST results are available for this feature:

BLAST of Carg10221 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6574775.1	1.8e-98	100.00	Protein-S-isoprenylcysteine O-methyltransferase B, partial [Cucurbita argyrosper...	[more]
KAG7013355.1	1.8e-98	100.00	Protein-S-isoprenylcysteine O-methyltransferase B, partial [Cucurbita argyrosper...	[more]
XP_023006693.1	5.2e-98	99.45	protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita maxima] >XP_02...	[more]
XP_022958895.1	5.2e-98	99.45	protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita moschata]	[more]
XP_023548051.1	7.5e-97	98.35	protein-S-isoprenylcysteine O-methyltransferase B-like [Cucurbita pepo subsp. pe...	[more]

BLAST of Carg10221 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W54	6.0e-73	70.72	Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana OX=370...	[more]
Q9FMW9	7.4e-71	68.51	Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana OX=370...	[more]
A2XX73	1.1e-69	66.48	Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. ...	[more]
Q7XSR9	1.1e-69	66.48	Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. ...	[more]
O60725	4.4e-39	44.75	Protein-S-isoprenylcysteine O-methyltransferase OS=Homo sapiens OX=9606 GN=ICMT ...	[more]

BLAST of Carg10221 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L5N1	2.5e-98	99.45	Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1H4S0	2.5e-98	99.45	Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1DSC7	2.4e-88	87.36	Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DPQ1	2.4e-88	87.36	Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G...	[more]
A0A6J1D2G4	1.5e-87	86.81	Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G...	[more]

BLAST of Carg10221 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08335.1	4.3e-74	70.72	Isoprenylcysteine carboxyl methyltransferase (ICMT) family	[more]
AT5G23320.1	5.2e-72	68.51	homolog of yeast STE14 A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007269	Isoprenylcysteine carboxyl methyltransferase	PFAM	PF04140	ICMT	coord: 68..159 e-value: 2.9E-27 score: 94.9
None	No IPR available	GENE3D	1.20.120.1630		coord: 1..181 e-value: 2.8E-35 score: 123.8
None	No IPR available	PANTHER	PTHR12714:SF19	PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE	coord: 2..182
None	No IPR available	PANTHER	PTHR12714	PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE	coord: 2..182
IPR025770	Protein-S-isoprenylcysteine O-methyltransferase	PROSITE	PS51564	SAM_ICMT	coord: 2..182 score: 24.778721

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10221-RA	Carg10221-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006481	C-terminal protein methylation
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0004671	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity