Carg10152 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10152
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Chaperone protein dnaJ 20
Location	Carg_Chr17: 456268 .. 457952 (+)
RNA-Seq Expression	Carg10152
Synteny	Carg10152

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTAGCTGCGGTTTGGCTTTATCAGGTACCGATTCACGGTGCTATTTACCGGCGATGCCTCCGATATTTTCTCCGATGAGAGCCTGTCCACCGGAAGGAAATTCGCAGGTTTTGTTCCCTAAATTGACACGGCCTCCTTCTGCTTGCGTTAGAGCAAAGGCCACGGTTGTCAACGAGGCGCTAGTCTCCGACGCCGTCGAGATAAGCTTCTACGAGCTTTTAGGAGTGCCGGAGTCGGGGTCGATGGTGGAGATTAAGCAGGCCTACAAGCAGATGGCTCGGAAATACCATCCGGACGTCTCGCCACCGGACCGGATCGAGGAGTATACAAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTATCGGATCCTGTTATGAGAGATTTGTATGATAGAGACATGGCGAGAGGTCTTCACCTTGCTTTCTCTGCTCGAAGACGGGGCTACAACCATGAGGTATGTTTTTTGGCCATTTTTGAGGTCAAAATCGGTTGCTGAATCAATTAGGATGTCTTCTCTACCTTAATTTATTGCAATTTAAATCATTATATTGAATTGATTTTTACTCGTCTTGAACAATTTCGCCTCCTCATCCTGAATGCTTGTTATTTTCCCTGACCTCTTCGTTTTTGCAACCTAGATTACTCCATGCACTCAGTCTGGTGTTTCATCTGTTTTTTTAATTTTCTGTCTTCGATTTAACAGTAGATGATGATTATAATCTGGACCACAGGTATATTCTCGATATGCAAATACTTTTTGAGTTTTTTTTACTAAAACAGTAGATTAACCATTAGCAACAGCTCTTCTAATTCTTCGACTATCTTCTAATTAAGCTAATCCTAATTCAAAAGACTTGTGTGCATCGAAACATGTATAATCTGGACCAAATCATGACTCCTACCTATATAACTTACATGACAGATATCGATTCTTTTAGTTTCATTATCAAAATTTCCTATATTAAATCTGTACTTATAATTCTCTGAATTGCTCAGGAAATGGATGAGAAAGGTGGGTGGAAGAGCCGTTGGCAGGATCAGCTATCAGAATTAAAGAGAAGAAGCATGAATAAAAATTCAAGAGCAAACATGTCATGGGGAGCTCGAATGCGCAGTCAAAGGGACGAGCTTCACAACGACGAAAACCCGTAATTTGGTTTTTCCCCTGCTCTGAACTTTCAACCTTCCTGCATATAGCTAATATTAATACTCTAGCTAGATACAGGACCTTAGCCTACTGATTACTAGAACTTCTACTTGTTGCTCTGTGAAACTCAAATTGCTTAGAGTCTGCATATTGGGAACTTGTTTCACAACGCATGTAAATCACTAGAAAACCAGCATCGAATGTGTGTGTGTATATATATATATATATATGGAAATTATAGTATCTCAGAGTAATCTATCAATGTTGAATTCATCGTTCCTGATTAGTCCTGGTAGATACCAATTTTGTTTCAGAATGTCTACTGTGCTTTTCTAAAATCACAGTCTTTGTTACCAATATTTTCTCTGTTTCCGTTCCACTAAAACCACTGTTAGAACATGATTCAGCTTTTTGTGTTTAGGTTTTAAAAAAGTTGCTAGGAGCCAAAAAACAAGTTGAAGAAAAAAACAAACTCATCTTTTGGAGTGCCACCATTAATGCAGATTACCATCCTTGTGATCAATAAT

mRNA sequence

ATGCGTAGCTGCGGTTTGGCTTTATCAGGTACCGATTCACGGTGCTATTTACCGGCGATGCCTCCGATATTTTCTCCGATGAGAGCCTGTCCACCGGAAGGAAATTCGCAGGTTTTGTTCCCTAAATTGACACGGCCTCCTTCTGCTTGCGTTAGAGCAAAGGCCACGGTTGTCAACGAGGCGCTAGTCTCCGACGCCGTCGAGATAAGCTTCTACGAGCTTTTAGGAGTGCCGGAGTCGGGGTCGATGGTGGAGATTAAGCAGGCCTACAAGCAGATGGCTCGGAAATACCATCCGGACGTCTCGCCACCGGACCGGATCGAGGAGTATACAAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTATCGGATCCTGTTATGAGAGATTTGTATGATAGAGACATGGCGAGAGGTCTTCACCTTGCTTTCTCTGCTCGAAGACGGGGCTACAACCATGAGGAAATGGATGAGAAAGGTGGGTGGAAGAGCCGTTGGCAGGATCAGCTATCAGAATTAAAGAGAAGAAGCATGAATAAAAATTCAAGAGCAAACATGTCATGGGGAGCTCGAATGCGCAGTCAAAGGGACGAGCTTCACAACGACGAAAACCCGTAATTTGGTTTTTCCCCTGCTCTGAACTTTCAACCTTCCTGCATATAGCTAATATTAATACTCTAGCTAGATACAGGACCTTAGCCTACTGATTACTAGAACTTCTACTTGTTGCTCTGTGAAACTCAAATTGCTTAGAGTCTGCATATTGGGAACTTGTTTCACAACGCATGTAAATCACTAGAAAACCAGCATCGAATGTGTGTGTGTATATATATATATATATATGGAAATTATAGTATCTCAGAGTAATCTATCAATGTTGAATTCATCGTTCCTGATTAGTCCTGGTAGATACCAATTTTGTTTCAGAATGTCTACTGTGCTTTTCTAAAATCACAGTCTTTGTTACCAATATTTTCTCTGTTTCCGTTCCACTAAAACCACTGTTAGAACATGATTCAGCTTTTTGTGTTTAGGTTTTAAAAAAGTTGCTAGGAGCCAAAAAACAAGTTGAAGAAAAAAACAAACTCATCTTTTGGAGTGCCACCATTAATGCAGATTACCATCCTTGTGATCAATAAT

Coding sequence (CDS)

ATGCGTAGCTGCGGTTTGGCTTTATCAGGTACCGATTCACGGTGCTATTTACCGGCGATGCCTCCGATATTTTCTCCGATGAGAGCCTGTCCACCGGAAGGAAATTCGCAGGTTTTGTTCCCTAAATTGACACGGCCTCCTTCTGCTTGCGTTAGAGCAAAGGCCACGGTTGTCAACGAGGCGCTAGTCTCCGACGCCGTCGAGATAAGCTTCTACGAGCTTTTAGGAGTGCCGGAGTCGGGGTCGATGGTGGAGATTAAGCAGGCCTACAAGCAGATGGCTCGGAAATACCATCCGGACGTCTCGCCACCGGACCGGATCGAGGAGTATACAAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTATCGGATCCTGTTATGAGAGATTTGTATGATAGAGACATGGCGAGAGGTCTTCACCTTGCTTTCTCTGCTCGAAGACGGGGCTACAACCATGAGGAAATGGATGAGAAAGGTGGGTGGAAGAGCCGTTGGCAGGATCAGCTATCAGAATTAAAGAGAAGAAGCATGAATAAAAATTCAAGAGCAAACATGTCATGGGGAGCTCGAATGCGCAGTCAAAGGGACGAGCTTCACAACGACGAAAACCCGTAA

Protein sequence

MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNEALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMNKNSRANMSWGARMRSQRDELHNDENP

Homology

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BLAST of Carg10152 vs. NCBI nr
Match: KAG6574846.1 (Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013422.1 Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415.6 bits (1067), Expect = 2.5e-112
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDENP 207
           KNSRANMSWGARMRSQRDELHNDENP
Sbjct: 181 KNSRANMSWGARMRSQRDELHNDENP 206

[Close]

BLAST of Carg10152 vs. NCBI nr
Match: XP_022959157.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 412.5 bits (1059), Expect = 2.1e-111
Identity = 204/206 (99.03%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDENP 207
           KNSRANMSWGARMR QRDELHND+NP
Sbjct: 181 KNSRANMSWGARMRRQRDELHNDDNP 206

[Close]

BLAST of Carg10152 vs. NCBI nr
Match: XP_023549024.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 403.3 bits (1035), Expect = 1.3e-108
Identity = 199/205 (97.07%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEM+EKGGWK+RWQDQLSELKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKNRWQDQLSELKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDEN 206
           KNSRANMSWGARMR QRDELH+D++
Sbjct: 181 KNSRANMSWGARMRRQRDELHDDDD 205

[Close]

BLAST of Carg10152 vs. NCBI nr
Match: XP_023006292.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 397.1 bits (1019), Expect = 9.1e-107
Identity = 196/206 (95.15%), Postives = 201/206 (97.57%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPPIFSPMRACP +GNSQVLFPKLTRPPSACVRAKATVV E
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVSDA+EISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEM+EKGGWKSRWQDQLSELKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDENP 207
           KNSRANMSWGARMR Q DELH+D+NP
Sbjct: 181 KNSRANMSWGARMRRQGDELHDDDNP 206

[Close]

BLAST of Carg10152 vs. NCBI nr
Match: XP_038875872.1 (chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 173/204 (84.80%), Postives = 185/204 (90.69%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPP FSP RA PP+G SQVLFPK  R PSACVR KATVVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPTFSPRRASPPDGTSQVLFPKSARHPSACVRVKATVVNE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VSD VE+SFYE+LGVPESGSMVEIKQAYKQMA KYHPDVSP DRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDDVEMSFYEVLGVPESGSMVEIKQAYKQMALKYHPDVSPRDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDP MR LYD DMA GLHLAFSARRRGYNHEEM+EK GWK+RW+DQLS LK+RSMN
Sbjct: 121 YETLSDPSMRALYDSDMAIGLHLAFSARRRGYNHEEMEEKSGWKNRWKDQLSGLKKRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDE 205
           K+SRA+MSWGARMR QRD+LH D+
Sbjct: 181 KDSRASMSWGARMRRQRDKLHEDD 204

[Close]

BLAST of Carg10152 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 2.6e-40
Identity = 89/163 (54.60%), Postives = 119/163 (73.01%), Query Frame = 0


Query: 38  VLFPKLTRPPSACVRAKATVVNEALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKY 97
           + +P  TR  S  ++++ T  ++  V  + ++SFY+LLGV ES ++ EIKQAYKQ+ARKY
Sbjct: 36  ISYPTRTRFSSTRIQSRLT--HDDPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKY 95

Query: 98  HPDVSPPDRIEEYTRKFIKVQEAYETLSDPVMRDLYDRDMARGLHLAFSARRRG-YNHEE 157
           HPDVSPPDR+EEYT +FI+VQEAYETLSDP  R LYDRD++ G   +FS RR+  Y+ E 
Sbjct: 96  HPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEV 155

Query: 158 MDEKGGWKSRWQDQLSELKRRSMNKNSRANMSWGARMRSQRDE 200
           ++EK  WK++WQ QLS L+RRS N+     MSW ARMR Q+ E
Sbjct: 156 VEEKSEWKAKWQTQLSGLRRRS-NQKDNNTMSWAARMRRQQQE 195

[Close]

BLAST of Carg10152 vs. ExPASy Swiss-Prot
Match: Q0III6 (DnaJ homolog subfamily B member 6 OS=Bos taurus OX=9913 GN=DNAJB6 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.8e-12
Identity = 36/74 (48.65%), Postives = 51/74 (68.92%), Query Frame = 0


Query: 69  ISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPV 128
           + +YE+LGV    S  +IK+AY+++A K+HPD +P ++ EE  RKF +V EAYE LSD  
Sbjct: 2   VDYYEVLGVQRHASAEDIKKAYRKLALKWHPDKNPENK-EEAERKFKQVAEAYEVLSDAK 61

Query: 129 MRDLYDRDMARGLH 143
            RD+YDR    GL+
Sbjct: 62  KRDIYDRYGKEGLN 74

[Close]

BLAST of Carg10152 vs. ExPASy Swiss-Prot
Match: O66921 (Chaperone protein DnaJ 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=dnaJ2 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-11
Identity = 33/72 (45.83%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 63  VSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYE 122
           ++ + +  +YE+LGVP + S  EIK+AY+++ RKYHPD+      EE   KF ++ EAY+
Sbjct: 1   MASSTKKDYYEILGVPRNASQEEIKKAYRRLVRKYHPDICKKPECEE---KFKEINEAYQ 60

Query: 123 TLSDPVMRDLYD 135
            LSDP  R LYD
Sbjct: 61  VLSDPEKRKLYD 69

[Close]

BLAST of Carg10152 vs. ExPASy Swiss-Prot
Match: P25491 (Mitochondrial protein import protein MAS5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDJ1 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-11
Identity = 37/74 (50.00%), Postives = 48/74 (64.86%), Query Frame = 0


Query: 68  EISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDP 127
           E  FY++LGVP + + VEIK+AY++ A KYHPD +P    EE   KF +   AYE LSDP
Sbjct: 4   ETKFYDILGVPVTATDVEIKKAYRKCALKYHPDKNPS---EEAAEKFKEASAAYEILSDP 63

Query: 128 VMRDLYDRDMARGL 142
             RD+YD+    GL
Sbjct: 64  EKRDIYDQFGEDGL 74

[Close]

BLAST of Carg10152 vs. ExPASy Swiss-Prot
Match: B9MJZ0 (Chaperone protein DnaJ OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) OX=521460 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 32/65 (49.23%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 71  FYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPVMR 130
           +YE+LGVP + +  EIK+AY+++A++YHPD +P ++  E   KF ++ EAYE LSDP  R
Sbjct: 7   YYEILGVPRNATEEEIKRAYRRLAKQYHPDANPGNK--EAEEKFKEINEAYEVLSDPEKR 66

Query: 131 DLYDR 136
            LYD+
Sbjct: 67  KLYDQ 69

[Close]

BLAST of Carg10152 vs. ExPASy TrEMBL
Match: A0A6J1H3S2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460232 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.0e-111
Identity = 204/206 (99.03%), Postives = 205/206 (99.51%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDENP 207
           KNSRANMSWGARMR QRDELHND+NP
Sbjct: 181 KNSRANMSWGARMRRQRDELHNDDNP 206

[Close]

BLAST of Carg10152 vs. ExPASy TrEMBL
Match: A0A6J1KVG4 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499073 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.4e-107
Identity = 196/206 (95.15%), Postives = 201/206 (97.57%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCYLPAMPPIFSPMRACP +GNSQVLFPKLTRPPSACVRAKATVV E
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVSDA+EISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEM+EKGGWKSRWQDQLSELKRRSMN
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDENP 207
           KNSRANMSWGARMR Q DELH+D+NP
Sbjct: 181 KNSRANMSWGARMRRQGDELHDDDNP 206

[Close]

BLAST of Carg10152 vs. ExPASy TrEMBL
Match: A0A6J1KFW2 (chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111494708 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.9e-86
Identity = 170/205 (82.93%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MR  GLALSGTDSRC++PAMPPIFSP RA P  GNSQVLFPK TR PS CVRAKA VVNE
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASPSVGNSQVLFPKSTRNPSLCVRAKAAVVNE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           ALVSDAVEISFY+LLG+PESGSM EIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYDLLGIPESGSMAEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDP MR LYDRDM  GLHLAFSARRRG N+EEM+ K GWKS WQDQLS L+RRSMN
Sbjct: 121 YETLSDPRMRALYDRDMLGGLHLAFSARRRG-NNEEMEVKSGWKSLWQDQLSGLRRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDEN 206
           K+S A+MSWGARMR QRDEL++DE+
Sbjct: 181 KDSGASMSWGARMRRQRDELNDDED 204

[Close]

BLAST of Carg10152 vs. ExPASy TrEMBL
Match: A0A6J1HHM6 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111464407 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.4e-84
Identity = 165/205 (80.49%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MR  GLALSGTDSRC++PAMP IFSP RA P  GNSQVLFPK TR PS CVRAKA VV+E
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VSDAVEISFY+LLG+PESGSM EIKQAYKQ+A KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDP MR LYDRDM  GLHLAFS+RRRG N+EEM+ K GWKSRWQDQLS L+RRSMN
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRG-NNEEMEVKSGWKSRWQDQLSGLRRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDEN 206
           K+S A+MSWGARMR QRDEL++D++
Sbjct: 181 KDSGASMSWGARMRRQRDELNDDDD 204

[Close]

BLAST of Carg10152 vs. ExPASy TrEMBL
Match: A0A5A7SVL1 (Chaperone protein dnaJ 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G00560 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.2e-83
Identity = 159/204 (77.94%), Postives = 178/204 (87.25%), Query Frame = 0


Query: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60
           MRSCGLALSGTDSRCY PA PPIFSP RA     NSQV F K  RPPSACVR KA  +N+
Sbjct: 1   MRSCGLALSGTDSRCYFPATPPIFSPRRA----SNSQVSFLKSPRPPSACVRVKAAFIND 60

Query: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
           A+VSDAVE+SFYELLGVPESGSM+EIKQAYKQMA KYHPDVSPP RIEEYTRKFIK+QEA
Sbjct: 61  AVVSDAVEVSFYELLGVPESGSMLEIKQAYKQMALKYHPDVSPPGRIEEYTRKFIKLQEA 120

Query: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180
           YETLSDP MR LYDRD+ARGL  AFS+R+R Y+HEEM+EK GW++RWQDQLS LKRRSMN
Sbjct: 121 YETLSDPNMRALYDRDLARGLQFAFSSRKRCYDHEEMEEKSGWRNRWQDQLSGLKRRSMN 180

Query: 181 KNSRANMSWGARMRSQRDELHNDE 205
           ++S+ANMSWGARMR +RDELH ++
Sbjct: 181 RDSKANMSWGARMRRERDELHKED 200

[Close]

BLAST of Carg10152 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 166.8 bits (421), Expect = 1.9e-41
Identity = 89/163 (54.60%), Postives = 119/163 (73.01%), Query Frame = 0


Query: 38  VLFPKLTRPPSACVRAKATVVNEALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKY 97
           + +P  TR  S  ++++ T  ++  V  + ++SFY+LLGV ES ++ EIKQAYKQ+ARKY
Sbjct: 36  ISYPTRTRFSSTRIQSRLT--HDDPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKY 95

Query: 98  HPDVSPPDRIEEYTRKFIKVQEAYETLSDPVMRDLYDRDMARGLHLAFSARRRG-YNHEE 157
           HPDVSPPDR+EEYT +FI+VQEAYETLSDP  R LYDRD++ G   +FS RR+  Y+ E 
Sbjct: 96  HPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEV 155

Query: 158 MDEKGGWKSRWQDQLSELKRRSMNKNSRANMSWGARMRSQRDE 200
           ++EK  WK++WQ QLS L+RRS N+     MSW ARMR Q+ E
Sbjct: 156 VEEKSEWKAKWQTQLSGLRRRS-NQKDNNTMSWAARMRRQQQE 195

[Close]

BLAST of Carg10152 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 128.3 bits (321), Expect = 7.3e-30
Identity = 64/113 (56.64%), Postives = 86/113 (76.11%), Query Frame = 0


Query: 38  VLFPKLTRPPSACVRAKATVVNEALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKY 97
           + +P  TR  S  ++++ T  ++  V  + ++SFY+LLGV ES ++ EIKQAYKQ+ARKY
Sbjct: 36  ISYPTRTRFSSTRIQSRLT--HDDPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKY 95

Query: 98  HPDVSPPDRIEEYTRKFIKVQEAYETLSDPVMRDLYDRDMARGLHLAFSARRR 151
           HPDVSPPDR+EEYT +FI+VQEAYETLSDP  R LYDRD++ G   +FS RR+
Sbjct: 96  HPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQ 146

[Close]

BLAST of Carg10152 vs. TAIR 10
Match: AT3G13310.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.4e-11
Identity = 43/117 (36.75%), Postives = 62/117 (52.99%), Query Frame = 0


Query: 24  FSPMRACPPEGNSQVLFPKLTRPPSACVRAKA---TVVNEALVSDAVE---ISFYELLGV 83
           FSP     P+  +   +    R P+     KA   T+  E  V+++V     S YELL V
Sbjct: 12  FSPGNCILPQQRTARFYSGTARFPTGAPSFKASAQTLNAEPAVTESVRRRVSSLYELLKV 71

Query: 84  PESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPVMRDLYD 135
            E+ S+ EIK AY+ +A+ YHPD S  D      R F+++ +AY TL+DP  R +YD
Sbjct: 72  NETASLTEIKTAYRSLAKVYHPDASESD-----GRDFMEIHKAYATLADPTTRAIYD 123

[Close]

BLAST of Carg10152 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 65.1 bits (157), Expect = 7.6e-11
Identity = 32/72 (44.44%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 70  SFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPVM 129
           S+Y++L VP+  S  +IK+AY+++A KYHPD +  +  EE TRKF ++  AYE LSD   
Sbjct: 26  SYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQGN--EEATRKFAEINNAYEVLSDEEK 85

Query: 130 RDLYDRDMARGL 142
           R++Y++    GL
Sbjct: 86  REIYNKYGEEGL 95

[Close]

BLAST of Carg10152 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.2e-10
Identity = 32/74 (43.24%), Postives = 45/74 (60.81%), Query Frame = 0


Query: 68  EISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDP 127
           +  +Y +LGV ++ +  EIK AY+++AR YHPDV+     EE   KF ++  AYE LSD 
Sbjct: 84  DADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSDD 143

Query: 128 VMRDLYDRDMARGL 142
             + LYDR    GL
Sbjct: 144 EKKSLYDRYGEAGL 154

The following BLAST results are available for this feature:

BLAST of Carg10152 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6574846.1	2.5e-112	100.00	Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022959157.1	2.1e-111	99.03	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata]	[more]
XP_023549024.1	1.3e-108	97.07	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006292.1	9.1e-107	95.15	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima]	[more]
XP_038875872.1	1.8e-91	84.80	chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Carg10152 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	2.6e-40	54.60	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
Q0III6	8.8e-12	48.65	DnaJ homolog subfamily B member 6 OS=Bos taurus OX=9913 GN=DNAJB6 PE=2 SV=1	[more]
O66921	1.5e-11	45.83	Chaperone protein DnaJ 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=dnaJ2 PE=...	[more]
P25491	1.5e-11	50.00	Mitochondrial protein import protein MAS5 OS=Saccharomyces cerevisiae (strain AT...	[more]
B9MJZ0	2.0e-11	49.23	Chaperone protein DnaJ OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DS...	[more]

BLAST of Carg10152 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H3S2	1.0e-111	99.03	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1KVG4	4.4e-107	95.15	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1KFW2	1.9e-86	82.93	chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1HHM6	1.4e-84	80.49	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A5A7SVL1	1.2e-83	77.94	Chaperone protein dnaJ 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Carg10152 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	1.9e-41	54.60	DNAJ-like 20	[more]
AT4G13830.1	7.3e-30	56.64	DNAJ-like 20	[more]
AT3G13310.1	3.4e-11	36.75	Chaperone DnaJ-domain superfamily protein	[more]
AT3G62600.1	7.6e-11	44.44	DNAJ heat shock family protein	[more]
AT2G22360.1	2.2e-10	43.24	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 109..129 score: 41.33 coord: 90..105 score: 46.22 coord: 72..90 score: 47.51
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 69..129 e-value: 1.5E-20 score: 84.3
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 71..134 e-value: 4.4E-20 score: 71.6
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 70..137 score: 19.018465
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 71..126 e-value: 5.95904E-20 score: 77.5857
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 68..197 e-value: 7.0E-22 score: 80.1
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 69..144
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..206
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 47..200
None	No IPR available	PANTHER	PTHR45090:SF4	CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTIC	coord: 47..200
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 114..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10152-RA	Carg10152-RA	mRNA