Carg10069 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10069
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerase V subunit 7
Location	Carg_Chr17: 990610 .. 991137 (+)
RNA-Seq Expression	Carg10069
Synteny	Carg10069

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCTCAAAGTGCAGCTGCCATGGAATGTCATAATCCCTGCTGAAAACTTAGATGCAAAGGGCTTGATGCTCCAAAGGTCTATTATAATCCGTCTGCTAGATGAATTTGCCACAAAAAAGGCCACAAAGGATCTCGGTTATTTCCTCGCAGTTACGACTCTAGAAAATATAGGAGAAGGGAAAGTTCGACAGACGGGCGACGTCTTGTTTCCAGTTGTTTTTAGTGGTATCACCTTCAAGCTTTATAGGGGAGAGATCTTGGAGGGGGTTGTTCACAAGGTTCTCAAGCATGGTGTTTTCTTGAGATGTGGCCCTGTTGAGAACATATATCTCTCTTATCTGAAAATGCCAGATTACCGCTATGTGCCGGGCGAGAATCCCCTGTTCATGAACGACAAGCTTTCAAAAATTGAGAAAGATGTAGTCGTACGTTTTATCGTCATTGGAACGAAGTGGCTCGAGGCAGAAAGGGAGTTTCAGGCACTGGTGAGTTTGGAGGGTGATTATCTAGGGCCAGTTTCTTAA

mRNA sequence

ATGTTTCTCAAAGTGCAGCTGCCATGGAATGTCATAATCCCTGCTGAAAACTTAGATGCAAAGGGCTTGATGCTCCAAAGGTCTATTATAATCCGTCTGCTAGATGAATTTGCCACAAAAAAGGCCACAAAGGATCTCGGTTATTTCCTCGCAGTTACGACTCTAGAAAATATAGGAGAAGGGAAAGTTCGACAGACGGGCGACGTCTTGTTTCCAGTTGTTTTTAGTGGTATCACCTTCAAGCTTTATAGGGGAGAGATCTTGGAGGGGGTTGTTCACAAGGTTCTCAAGCATGGTGTTTTCTTGAGATGTGGCCCTGTTGAGAACATATATCTCTCTTATCTGAAAATGCCAGATTACCGCTATGTGCCGGGCGAGAATCCCCTGTTCATGAACGACAAGCTTTCAAAAATTGAGAAAGATGTAGTCGTACGTTTTATCGTCATTGGAACGAAGTGGCTCGAGGCAGAAAGGGAGTTTCAGGCACTGGTGAGTTTGGAGGGTGATTATCTAGGGCCAGTTTCTTAA

Coding sequence (CDS)

ATGTTTCTCAAAGTGCAGCTGCCATGGAATGTCATAATCCCTGCTGAAAACTTAGATGCAAAGGGCTTGATGCTCCAAAGGTCTATTATAATCCGTCTGCTAGATGAATTTGCCACAAAAAAGGCCACAAAGGATCTCGGTTATTTCCTCGCAGTTACGACTCTAGAAAATATAGGAGAAGGGAAAGTTCGACAGACGGGCGACGTCTTGTTTCCAGTTGTTTTTAGTGGTATCACCTTCAAGCTTTATAGGGGAGAGATCTTGGAGGGGGTTGTTCACAAGGTTCTCAAGCATGGTGTTTTCTTGAGATGTGGCCCTGTTGAGAACATATATCTCTCTTATCTGAAAATGCCAGATTACCGCTATGTGCCGGGCGAGAATCCCCTGTTCATGAACGACAAGCTTTCAAAAATTGAGAAAGATGTAGTCGTACGTTTTATCGTCATTGGAACGAAGTGGCTCGAGGCAGAAAGGGAGTTTCAGGCACTGGTGAGTTTGGAGGGTGATTATCTAGGGCCAGTTTCTTAA

Protein sequence

MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGEGKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDYRYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS

Homology

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BLAST of Carg10069 vs. NCBI nr
Match: XP_022958784.1 (DNA-directed RNA polymerase V subunit 7 [Cucurbita moschata] >XP_022958785.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita moschata] >XP_022958786.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita moschata] >XP_022958787.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita moschata] >KAG6574936.1 DNA-directed RNA polymerase V subunit 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013504.1 DNA-directed RNA polymerase V subunit 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 354.4 bits (908), Expect = 5.7e-94
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. NCBI nr
Match: XP_023006755.1 (DNA-directed RNA polymerase V subunit 7 [Cucurbita maxima] >XP_023006756.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita maxima] >XP_023006757.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita maxima] >XP_023548587.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita pepo subsp. pepo] >XP_023548589.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita pepo subsp. pepo] >XP_023548590.1 DNA-directed RNA polymerase V subunit 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.0 bits (907), Expect = 7.5e-94
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPV+FSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVIFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

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BLAST of Carg10069 vs. NCBI nr
Match: XP_022138329.1 (DNA-directed RNA polymerase V subunit 7 [Momordica charantia])

HSP 1 Score: 352.1 bits (902), Expect = 2.8e-93
Identity = 173/175 (98.86%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIP+ENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPSENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENP+FMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPVFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. NCBI nr
Match: XP_004150402.1 (DNA-directed RNA polymerase V subunit 7 [Cucumis sativus] >XP_038875656.1 DNA-directed RNA polymerase V subunit 7 [Benincasa hispida] >XP_038875657.1 DNA-directed RNA polymerase V subunit 7 [Benincasa hispida] >KGN61629.1 hypothetical protein Csa_005986 [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 3.7e-93
Identity = 172/175 (98.29%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPV+FSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVIFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENP+F+NDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPVFLNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. NCBI nr
Match: XP_008465191.1 (PREDICTED: DNA-directed RNA polymerase V subunit 7 [Cucumis melo] >KAA0057517.1 DNA-directed RNA polymerase V subunit 7 [Cucumis melo var. makuwa] >TYK08198.1 DNA-directed RNA polymerase V subunit 7 [Cucumis melo var. makuwa])

HSP 1 Score: 350.5 bits (898), Expect = 8.3e-93
Identity = 172/175 (98.29%), Postives = 174/175 (99.43%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPV FSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVTFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENP+F+NDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPVFLNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. ExPASy Swiss-Prot
Match: A6QRA1 (DNA-directed RNA polymerase V subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPE7 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 5.1e-77
Identity = 128/176 (72.73%), Postives = 162/176 (92.05%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNV+IPAEN+DAKGLML+R+I++ LL+ FA+KKATK+LGY++AVTTL+ IGE
Sbjct: 1   MFLKVQLPWNVMIPAENMDAKGLMLKRAILVELLEAFASKKATKELGYYVAVTTLDKIGE 60

Query: 61  GKVRQ-TGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPD 120
           GK+R+ TG+VLFPV+FSG+TFK+++GEI+ GVVHKVLKHGVF+RCGP+EN+YLSY KMPD
Sbjct: 61  GKIREHTGEVLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPD 120

Query: 121 YRYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           Y+Y+PGENP+FMN+K S+I+ +  VR +VIG KW+E EREFQAL SLEGDYLGP+S
Sbjct: 121 YKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWMEVEREFQALASLEGDYLGPLS 176

[Close]

BLAST of Carg10069 vs. ExPASy Swiss-Prot
Match: Q8LE42 (DNA-directed RNA polymerase IV subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPD7 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 3.7e-59
Identity = 107/174 (61.49%), Postives = 142/174 (81.61%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MF+KV+LPW+V IPAE++D  GLMLQR+I+IRLL+ F+ +KATKDLGY +  T LENIGE
Sbjct: 1   MFIKVKLPWDVTIPAEDMDT-GLMLQRAIVIRLLEAFSKEKATKDLGYLITPTILENIGE 60

Query: 61  GKVR-QTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPD 120
           GK++ QTG++ FPVVF+GI FK+++GEI+ GVVHKV K GVFL+ GP E IYLS++KMP 
Sbjct: 61  GKIKEQTGEIQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMKMPG 120

Query: 121 YRYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGP 174
           Y ++PGENP FMN  +S+I+    VRF+V+ T+W EAE++F AL S++GD LGP
Sbjct: 121 YEFIPGENPFFMNQYMSRIQIGARVRFVVLDTEWREAEKDFMALASIDGDNLGP 173

[Close]

BLAST of Carg10069 vs. ExPASy Swiss-Prot
Match: Q6NML5 (DNA-directed RNA polymerase subunit 7-like protein OS=Arabidopsis thaliana OX=3702 GN=NRPB7L PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.9e-24
Identity = 74/193 (38.34%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MF +V++  +V I A++L+  G    + I+ RLL +   +KA ++ G++L +T L++IG 
Sbjct: 1   MFSEVEMARDVAICAKHLN--GQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIGN 60

Query: 61  GK--------VRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYL 120
            K          Q   + FPV F+  TF   RG+IL+G V KVL +G F+R GP+   YL
Sbjct: 61  NKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYL 120

Query: 121 SYLKMPDYRYV-----PGENPLFMNDKLSKIEKDVVVRFIVIGTKWLE----AEREFQAL 176
           S LKMP Y YV       E P F  D LSKI   VVVRF V+  ++ E       ++  L
Sbjct: 121 SLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVL 180

[Close]

BLAST of Carg10069 vs. ExPASy Swiss-Prot
Match: P46279 (DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 50/131 (38.17%), Postives = 79/131 (60.31%), Query Frame = 0


Query: 47  GYFLAVTTLENIGEGKVRQ-TGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCG 106
           G+ +AVT +ENIG+G +R  TG V FPV +  + F+ ++GEILE VV  V K G F   G
Sbjct: 43  GFVVAVTGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAG 102

Query: 107 PVENIYLSYLKMPDYRYVPGENPLF-MNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALV 166
           PV+    ++L   D  +  G+ P +  +D   KI+KD  VR  +IGT+ ++A   F  + 
Sbjct: 103 PVQIFVSNHLIPDDMEFQSGDMPNYTTSDGSVKIQKDSEVRLKIIGTR-VDATEIF-CIG 162

Query: 167 SLEGDYLGPVS 176
           +++ D+LG ++
Sbjct: 163 TIKDDFLGVIN 171

[Close]

BLAST of Carg10069 vs. ExPASy Swiss-Prot
Match: P38421 (DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB7 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.0e-14
Identity = 53/165 (32.12%), Postives = 87/165 (52.73%), Query Frame = 0


Query: 21  KGLMLQRSIIIRLLDEFATKKATKDL--------GYFLAVTTLENIGEGKVRQ-TGDVLF 80
           + + L      R L E    K  KD+        G+ +A+T ++ IG+G +R  TG V F
Sbjct: 9   RNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIRDGTGFVTF 68

Query: 81  PVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDYRYVPGENPLF- 140
           PV +  + F+ ++GEILE VV  V K G F   GPV+     +L   D  +  G+ P + 
Sbjct: 69  PVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQAGDMPNYT 128

Query: 141 MNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
            +D   KI+K+  VR  +IGT+ ++A   F  + +++ D+LG ++
Sbjct: 129 TSDGSVKIQKECEVRLKIIGTR-VDATAIF-CVGTIKDDFLGVIN 171

[Close]

BLAST of Carg10069 vs. ExPASy TrEMBL
Match: A0A6J1H315 (DNA-directed RNA polymerase V subunit 7 OS=Cucurbita moschata OX=3662 GN=LOC111459953 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.8e-94
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. ExPASy TrEMBL
Match: A0A6J1KYN2 (DNA-directed RNA polymerase V subunit 7 OS=Cucurbita maxima OX=3661 GN=LOC111499394 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 3.6e-94
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPV+FSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVIFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. ExPASy TrEMBL
Match: A0A6J1C9U9 (DNA-directed RNA polymerase V subunit 7 OS=Momordica charantia OX=3673 GN=LOC111009541 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.4e-93
Identity = 173/175 (98.86%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIP+ENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPSENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENP+FMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPVFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. ExPASy TrEMBL
Match: A0A0A0LKQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G193330 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.8e-93
Identity = 172/175 (98.29%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPV+FSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVIFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENP+F+NDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPVFLNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. ExPASy TrEMBL
Match: A0A5D3CAJ0 (DNA-directed RNA polymerase V subunit 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00140 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 4.0e-93
Identity = 172/175 (98.29%), Postives = 174/175 (99.43%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE
Sbjct: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60

Query: 61  GKVRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120
           GKVRQTGDVLFPV FSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY
Sbjct: 61  GKVRQTGDVLFPVTFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPDY 120

Query: 121 RYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           RYVPGENP+F+NDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS
Sbjct: 121 RYVPGENPVFLNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 175

[Close]

BLAST of Carg10069 vs. TAIR 10
Match: AT4G14660.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 288.5 bits (737), Expect = 3.6e-78
Identity = 128/176 (72.73%), Postives = 162/176 (92.05%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MFLKVQLPWNV+IPAEN+DAKGLML+R+I++ LL+ FA+KKATK+LGY++AVTTL+ IGE
Sbjct: 1   MFLKVQLPWNVMIPAENMDAKGLMLKRAILVELLEAFASKKATKELGYYVAVTTLDKIGE 60

Query: 61  GKVRQ-TGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPD 120
           GK+R+ TG+VLFPV+FSG+TFK+++GEI+ GVVHKVLKHGVF+RCGP+EN+YLSY KMPD
Sbjct: 61  GKIREHTGEVLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPD 120

Query: 121 YRYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176
           Y+Y+PGENP+FMN+K S+I+ +  VR +VIG KW+E EREFQAL SLEGDYLGP+S
Sbjct: 121 YKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWMEVEREFQALASLEGDYLGPLS 176

[Close]

BLAST of Carg10069 vs. TAIR 10
Match: AT3G22900.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 229.2 bits (583), Expect = 2.6e-60
Identity = 107/174 (61.49%), Postives = 142/174 (81.61%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MF+KV+LPW+V IPAE++D  GLMLQR+I+IRLL+ F+ +KATKDLGY +  T LENIGE
Sbjct: 1   MFIKVKLPWDVTIPAEDMDT-GLMLQRAIVIRLLEAFSKEKATKDLGYLITPTILENIGE 60

Query: 61  GKVR-QTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYLSYLKMPD 120
           GK++ QTG++ FPVVF+GI FK+++GEI+ GVVHKV K GVFL+ GP E IYLS++KMP 
Sbjct: 61  GKIKEQTGEIQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMKMPG 120

Query: 121 YRYVPGENPLFMNDKLSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGP 174
           Y ++PGENP FMN  +S+I+    VRF+V+ T+W EAE++F AL S++GD LGP
Sbjct: 121 YEFIPGENPFFMNQYMSRIQIGARVRFVVLDTEWREAEKDFMALASIDGDNLGP 173

[Close]

BLAST of Carg10069 vs. TAIR 10
Match: AT4G14520.1 (DNA-directed RNA polymerase II-related )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 74/193 (38.34%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MF +V++  +V I A++L+  G    + I+ RLL +   +KA ++ G++L +T L++IG 
Sbjct: 1   MFSEVEMARDVAICAKHLN--GQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIGN 60

Query: 61  GK--------VRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYL 120
            K          Q   + FPV F+  TF   RG+IL+G V KVL +G F+R GP+   YL
Sbjct: 61  NKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYL 120

Query: 121 SYLKMPDYRYV-----PGENPLFMNDKLSKIEKDVVVRFIVIGTKWLE----AEREFQAL 176
           S LKMP Y YV       E P F  D LSKI   VVVRF V+  ++ E       ++  L
Sbjct: 121 SLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVL 180

[Close]

BLAST of Carg10069 vs. TAIR 10
Match: AT4G14520.2 (DNA-directed RNA polymerase II-related )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 74/193 (38.34%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MF +V++  +V I A++L+  G    + I+ RLL +   +KA ++ G++L +T L++IG 
Sbjct: 1   MFSEVEMARDVAICAKHLN--GQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIGN 60

Query: 61  GK--------VRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYL 120
            K          Q   + FPV F+  TF   RG+IL+G V KVL +G F+R GP+   YL
Sbjct: 61  NKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYL 120

Query: 121 SYLKMPDYRYV-----PGENPLFMNDKLSKIEKDVVVRFIVIGTKWLE----AEREFQAL 176
           S LKMP Y YV       E P F  D LSKI   VVVRF V+  ++ E       ++  L
Sbjct: 121 SLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVL 180

[Close]

BLAST of Carg10069 vs. TAIR 10
Match: AT4G14520.3 (DNA-directed RNA polymerase II-related )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 74/193 (38.34%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 1   MFLKVQLPWNVIIPAENLDAKGLMLQRSIIIRLLDEFATKKATKDLGYFLAVTTLENIGE 60
           MF +V++  +V I A++L+  G    + I+ RLL +   +KA ++ G++L +T L++IG 
Sbjct: 1   MFSEVEMARDVAICAKHLN--GQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIGN 60

Query: 61  GK--------VRQTGDVLFPVVFSGITFKLYRGEILEGVVHKVLKHGVFLRCGPVENIYL 120
            K          Q   + FPV F+  TF   RG+IL+G V KVL +G F+R GP+   YL
Sbjct: 61  NKNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYL 120

Query: 121 SYLKMPDYRYV-----PGENPLFMNDKLSKIEKDVVVRFIVIGTKWLE----AEREFQAL 176
           S LKMP Y YV       E P F  D LSKI   VVVRF V+  ++ E       ++  L
Sbjct: 121 SLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVL 180

The following BLAST results are available for this feature:

BLAST of Carg10069 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958784.1	5.7e-94	100.00	DNA-directed RNA polymerase V subunit 7 [Cucurbita moschata] >XP_022958785.1 DNA...	[more]
XP_023006755.1	7.5e-94	99.43	DNA-directed RNA polymerase V subunit 7 [Cucurbita maxima] >XP_023006756.1 DNA-d...	[more]
XP_022138329.1	2.8e-93	98.86	DNA-directed RNA polymerase V subunit 7 [Momordica charantia]	[more]
XP_004150402.1	3.7e-93	98.29	DNA-directed RNA polymerase V subunit 7 [Cucumis sativus] >XP_038875656.1 DNA-di...	[more]
XP_008465191.1	8.3e-93	98.29	PREDICTED: DNA-directed RNA polymerase V subunit 7 [Cucumis melo] >KAA0057517.1 ...	[more]

BLAST of Carg10069 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A6QRA1	5.1e-77	72.73	DNA-directed RNA polymerase V subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPE7...	[more]
Q8LE42	3.7e-59	61.49	DNA-directed RNA polymerase IV subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]
Q6NML5	2.9e-24	38.34	DNA-directed RNA polymerase subunit 7-like protein OS=Arabidopsis thaliana OX=37...	[more]
P46279	1.5e-15	38.17	DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1	[more]
P38421	8.0e-14	32.12	DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]

BLAST of Carg10069 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H315	2.8e-94	100.00	DNA-directed RNA polymerase V subunit 7 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KYN2	3.6e-94	99.43	DNA-directed RNA polymerase V subunit 7 OS=Cucurbita maxima OX=3661 GN=LOC111499...	[more]
A0A6J1C9U9	1.4e-93	98.86	DNA-directed RNA polymerase V subunit 7 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0LKQ1	1.8e-93	98.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G193330 PE=4 SV=1	[more]
A0A5D3CAJ0	4.0e-93	98.29	DNA-directed RNA polymerase V subunit 7 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of Carg10069 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14660.1	3.6e-78	72.73	RNA polymerase Rpb7-like, N-terminal domain	[more]
AT3G22900.1	2.6e-60	61.49	RNA polymerase Rpb7-like, N-terminal domain	[more]
AT4G14520.1	2.1e-25	38.34	DNA-directed RNA polymerase II-related	[more]
AT4G14520.2	2.1e-25	38.34	DNA-directed RNA polymerase II-related	[more]
AT4G14520.3	2.1e-25	38.34	DNA-directed RNA polymerase II-related	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	GENE3D	3.30.1490.120		coord: 1..83 e-value: 3.4E-15 score: 58.0
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	SUPERFAMILY	88798	N-terminal, heterodimerisation domain of RBP7 (RpoE)	coord: 1..80
None	No IPR available	GENE3D	2.40.50.140		coord: 84..174 e-value: 3.1E-24 score: 86.6
None	No IPR available	PANTHER	PTHR12709:SF3	DNA-DIRECTED RNA POLYMERASE V SUBUNIT 7	coord: 1..175
IPR045113	RNA polymerase Rpb7-like	PANTHER	PTHR12709	DNA-DIRECTED RNA POLYMERASE II, III	coord: 1..175
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 84..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10069-RA	Carg10069-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006352	DNA-templated transcription, initiation
biological_process	GO:0006412	translation
cellular_component	GO:0022627	cytosolic small ribosomal subunit
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003727	single-stranded RNA binding
molecular_function	GO:0003735	structural constituent of ribosome