Carg10061 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10061
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionacylamino-acid-releasing enzyme isoform X1
LocationCarg_Chr17: 1054367 .. 1063037 (+)
RNA-Seq ExpressionCarg10061
SyntenyCarg10061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATCTGATGGCGTTGGCTAGCACTTACTCCATTGGTCGGATCAACTTCCTTCGTCTTCCCCTCTTCGCCACTGCTGCTACTGCTCCTCATTCTCAGAGCTCTCAATTTCTCAGAGCCACCTGCCTCTTCTCGTAAGCTCCCTCTCTCTCATTTCTTATCGCTATGTTGCCTTTTGCCGTCAAATTCTGTAGAGTTTCTTCTTTGTTTCCTTTGTGTAATGCTTTTGTTCTATTTACAAGCATTTTTTAATCCGAAAATCCACGGCAAGAAACTAACATAAGTTTCGAATTACTGCTCTCTGGTGGTTAAAACTCGATCCATTGTTGAGTTATTTGTTGTATTTGGTGTAGTTTCGATGAATTTGCTTTGCATGTCCACTTTGATTCATCGGTTTTCATTACTAATGTAGTTATGTAAAAAAAATTCAATTATCATATATGGATGGGAAAATAATGGTTGAAAACGGATGTTCTGCTCTAAAAAATCTGATATTCTAGCAGACCGATAATTTAATCGAGCTTGTGTTTGAAGCTATTGATCAATCTGGTATAAGTTGAGAAGGTGATGAAATGTAACCCTCAGAATTGTTATAGATGCTAATTACAAATTACAATCTGAAATGATGATGTAATTCAGTTTGTTTGAGCATTGGTTGAATGGTTACGAATGTGAGCTGTGGGTCTTTTGAGGTTTGATTAGAAGGAAGTTCACATGGTTTTCTTTTTGTGAGTAAGGGGTTGATTGGGCTTGTAGATAAAAGCTTTGATACCATGCCAAGTTTCAACGAATTTACTAAGTGATTCTACTTCATTCGGTCAGTTTTGTAAAATGCACATGTAATTCATTGATTGATGAACTTGTAGGGCAGAGAAAGAAAGAAGTCGGGTGTGTCAATTTTATAGAATCTGAAAATTTGGTAAATCAGTGATTCGTGTGCCTATCTTGGCTGCTTACAACTTCTTTATTATTTTCTTTAACATTGTCATCATTGTTTGCGGAATTAATGTTGCAGTAGAAAACTGTCAACTTCCTTGGCCATGGACGGTTCAAAGATAAGTGCAGTTGTTGAGGAGTTTCCATCTGGGATTGATCCAGCAACTGAGGAAGAATATGTAGAACAGTCCAAATTACTTGAAGAATTCACAAAAATTCCTAACTTTGATAAGGCATGGACGTTTAAGTCTGATAGTGGTATGATTTCTATTGTTTTTGCTAGTGCATTTTCCACATGTGTGCGGTTGTGCCTTTTGTCACAAAGTTATGTGCCTGGCCATGTTAGTTCTATTTCAACACGACATTTTTAACAGGAGACTCCGTGGTAACGTTTTCAATTAGCCAAGCAAATCTTCTAGCAAATAAGAAGAGAAAATATACTTTGTCAGCTCACATTTCCGAGGGAAATGACGACAGTGAAGTAAACTTTAAATGGACGCCATTCCCTGTTGAAATGGTTGGAATCTCTACAATAGTGCCATCGCCAGCAGGCTCAAAATTTCTTGCAGTTCGTAATCCTGAAAATGATTCTCCAGTTCAATTGGAAATTTGGAGTGCAGGTCAACTTGAAAAGGAGTTTCACATCCCCCAATCTGTTCATGGCTCAATATATACTGATGGATGGTAATGACTCGGTTGTACATCTTTTTAATTGTGAATTTTATGTTAAATCAACGTAGACTTTTATGTTAAATCAACGTAGACTTTGTAATTATCATTTTCACTCTCTTGTGTCTCTTAAAATAAAGGAGAGAAAAAAAAAAGCGATATTCTTTCTTTACACTTCCTTGGATAAAAGTTATTCATCAACATGTACTAATTTTGCTGGTGAGTGGCTCTATAGCATCTGTAATATCAGAAGTAGCAAGAACTGAGTCATAATGGTATATGGCTGGTTGTTTTTGGTAAATAGTTTATATCTCAGGATCATCGACCACTAGATGTGAATTTCTATTTTCTTTTTATATTATAGGTTCGAGGGAATCTCTTGGAACTCAAATGAAACTTACATTGCTTATATTGCGGAGGAACCTTCTCCCTCCAAACCAACGTTTACTCTTTCTGGATATCAAAAAGGTTGCTCAACAGACAAGGATTCTACCAATTGGAAAGGTCAAGGGGATTTTAAGGAAGAATGGGGGGAAGCTTATGCAGGTAAAAGGCAGCCTGCTCTCTTTGTCGTCAATATTGACAGGTGTGGTTCAACTGTTTCCTGTATTCATATTGACAAAATAAGGTACATTTTAAAATTTTGAAAACTTCGTTGAGTATTCTTTTTTTTTTTGTTCTTTAACAGTGGAGAGGTAAAGCATGTGAAAATAAGCGAGCATTCTTTGAGTTTGGGTCAAGTTGTATGGGCTCCATCTATTGGCGAGCATCAATATTTGGTTTTTGTTGGATGGTCACCAGAGCCTCGAAAACTTGGTATGATCTACTGTTATAATCGGCCTTGTGCATTATATGCAGTTAAGGCCCCGGAGTTTGGATCAGAAGTCAATGAACATAAACTCAAGTGCGTTATACATTTTCTCTTCTCCTGATGATGGATTTTTTCTAATATCATTACAGTCGGAACATTCTAAATCTTTCAATGTTGTCCAGAGATGAATCAAAAGAGGATTTTCCCATCTATAATCTAACTCAGAACATAAGCAGTGCCTTTTCTCCTCGGTTCAGGTATATTTTTATCTCATTGCCTTCTGTTGTTTGTTTGCTTAAATTATTCTTCTTTTTCCTCTTTCTTACTAGCCTCCACATCTAATACAGTACCTTATAACTTAACTTCTGTAAATTCTTCTTCAAATTTGAATGAAAAATATAAGTGGCTACAGGCCACTTTGATATATATAATGAGCGGAGGCTAAAATCGTACTCTATTTCTGTTCAACTGTTTGCATGCGTTTCTAGTTCCAGAACTCTTTACAACCCTAGTGAGCATGACGCAACTTCTTTTGTGTTGTGATTTTAAAAATTTTATTTATTACATTAGGGAATATCAAGCGTGTTTTAATAGATAAATAACAGGTTAAGGCTGTTGAGTTAAATAACAGGTTAAGGCTGTATGTCATTTAATCATATTCCTCTTTTTAATTACATCTATATTGTTGCTTTAGAAGGTATTTCATGCTTATGGCATCAATTTAGACCATTTTATTTGATGTGTACATACTTCCCATCGATCAATTTTTCTAACTACTCTTATTGTTCATCGATTATCATGGAACCTTTATTTTATTGGCATGTCTATTCACTAACGCAAAAGATGAGCAGTAGCATCGTAACTGATTTATGAATGTTAATGTCTTCCAGCCCAGATGGAAAGTCACTTGTATTTTTATCTGCACACTCTTCTGTGAACACTGGCGCCCATTCCGCAACAAATTCTCTTCACAGAATCGACTGGCCTAGAGATGGGAAACTTAGTTTTAGTGAAACTATTGTTGATGTGGTGAGGTCTTGCATGCCCTTTTCTTTTCTAAATATAAAATTAATGATTAACGTGAAAATCCGTTGGCCAATTAATTTTCTTTGAACTAGTTTCTTCTGTGTGATGGTGTAACTGTACATGTTACAAATATTAGGGCATGTGTCAAATAGGTGTCGCACATGTGCGGGGGTGCTTTGAGAATGAAATACATATAAAATATTGTCTTTAAATTTCCAATTTTTTGTTAAGATGTTCAACCATTAAGAAAATTGATATGCTAGCTTGGCTATCAAGAATACTTTGACATAAAATATTGTATCTTAAAAGCTTAAGCATCATATCAACCTAAGCATAACTCAACTAATTAAGACGTATACTCTTGACCAAAGAAGTTAGTGGTTGAAATTCCTACGTGTTGGACTCAAATGCTCAAGTATTATGAACTAAAACAAATTCAATTTTTTTGCATGAGAATCACTAAAATGTATAAGAGTTCATTTTTGTGTGAGGCTGTTTTTTTTTTCTTTGTTTGTCCTTGTACATTCTGTCACTCTCAATGAAAGTTTGCAATGCTCAATTAGATGACGTTTTAATTGCTAAGTAAGACGTGCTTTGAAGTTTTCTTGTTTATTTACAAGTTATTTTTCTTAACAAATTTTTAAAATAAAATTTTGAAGGTTCCTGTGGTGCACTGGGCCAAGGACGACCTCTTTCCTGGCCTCTATTCACATGGTTTGCTGGCTAATCCATGGTTTTCTGATGGACACACTATTATTATAACAACCATATGGCGTAGCAGGAGTGCAATTGTCTCTATAGATATCTTAAGGCAATCTGCATAATTCTGAAGGAAATTATCGTTAATTCTGCGCTAAATTTTTGTTCTCTTATCTGATGACATTTTGTTTATTGAAAATAACAGCGGTGAAGTCTCATGCGTCAGTCCTGACCACACAGACTATTCTTGGAATGTTCTTGCTCTAGATGGAGACAACGTTATTGCTGGTAATGTGGAAATGAGATCTTTACGATAAGCTTGCTTGATGGAACTGATTTTTAACATTTGTAAGTACCCGTCTGCAGTTCGTAGCGGCCCAGTGACTATTCCTGGACTCAAGTATGGTTTTCTTATTGAGACAGAAACTAAGGATGTTGTATGGAATTGGGTCGATGTATTAAATCCTATGTTTAATTGCTCTACGAAGGTTATTGTTTATTTTTTATTTTTTTTAATAAATAAAGATACCCTGAAGGCCATTCCAAAACTTCCGTGATTAAGTAAACCAACACTTCATACAGGCTAGAACTCTACTCTCAAATATCTTGTTCAGTATATTGAAGATTCCAGTCAAGGATGTTTCCAACTGCGTGACAAAAGGTACTGTACAAACGATTTATTTACATTTTTATTAATAATGTCCGTTGATTATCATAATAATTCTTTGTTAATATTGCAACATAACAAAGACATCAATGGCCTTTTGAGAGGGCATCCGCTATCCCAAAGTCTCTCACTGAACAATCTCTAAAATAATTCTCCCCCTCTAAAATCTCCAACTCCCTCTATTTATATCAAAGTCACTGCTAGCTAATTATCTCTAGTCCTTACTATTATCCTATTGATGTTTCCACTATATATCCCCATTAGTTCCTGCATTTTGGTGACCTTCCCGGAGGTAATGTTGGTTTCCTTTTGTCTATTTTTATTATCAAACATAGGGCATTTTTTAGTTTATAAAACACAAATGAAGACGTGAATTGTGTTTCTTCTAAAGTTTTTTCACCTCAAAACTGATGAAACCCATTTTTGCTAGTTCAGTTCACTTAGTAAACTTTCTTTCCTTATAGTACTGAATAATCAAATCATTTCAGGTGCTAGTGAACCTTTTGAAGCTATCTTTGTAGCTCCGTTACCCAAAGGTAATGAGTTGAAACCACTTATTGTTAACCTTCATGGCGGTCCACATTCTACATCAACATCAAGCTTCTCGAAGTCCTTGGCATTTCTCGCTGCCGTAGGATTCAATCTTTTGATTGTGAACTATAGGTAGGTTTATTTTCTTCCCTCGGATCTTGATTTGGGTGGAATTGCACCTGGAATTTTTTTCAATGAAGGTCGGGTCCAGTCAAATTAATCAACTTTCAGTTGGTGGTGTATATAGATATATGAGAAGCTTATATCTCAACTCCCTTTCTCTCAAGTTTATTTGCTACTTGAGTTTTATTATTCAAAACCCCAGTTAAGCATTACCGTTATCTTTTGGACAAAAAGGATTGATTAGGTTTAATTATTGCACATTCAATGTTTAATTGGCTTATTTGAGAGCTACTTTGGAAGAAGTCATTTTCCCGTAAAGTGAGATTGAGGTGAATGGAAATTATGTTTTATTAATCAGCAGATATTGCATCACTGCGTGACGAGCATTTTTTCATTTCTCTGGCTTCTTTGATTTGGTATCTCAGAGGTTCATTGGGGTTCGGTGAGGATGCATTGCAATCTCTTCCTGGAAAAATTGGGTCTCAGGTACTAGTTTTAATGATGAATTTAGTTTTTTTTTTTTAATATTTATTTATCTATTCTTAGTCTTTTTGTGTGAATTTTCGTTTTACTTCTATCGTGATGACCGTTTTTCTCAGAATGCCACTGGATAGAATGTTTTATTTATCTTTTATATATTTTTGCTCAGGATGTCAATGATGTACTTGCCGCATTAGATCACATTATCGACCAGGGACTTGCCAATCCAGCTAAAGTTGCAGTTCTTGGCGGTTCTCATGGAGGTTTTTTGACAACTCATTTGATTGGCCAGGTTTTGTACTTAACGCCACTTTTATAGCTCACATTTTCTGAAATTAAATCCCGTGTTTGATTGATGGTTTTATGTGGAATTTAGATTCTGCGTGATTTATGGAGGATTTTGCATCTGTTGATGCTTTTCACGATATTTTCTATATCTAATTTTTCTCCAGCGCTACCCTTGAAAACATGATGGTGCACACTACGCATCATTCCACTAATCTGGTGATATTTTGACTTGCTTTTTTTTCTCTATCACAATAGTTTTCACTACCTAAAAATTAATTTTCATAAAGTCAAGTTAGATTTGATTTTCAAAATCTTAGAAGTACTTAAAAAGAACATTTTAAAATACTTTTTCGATGAAGCATTCATTTACTTAGTGCTTTTGAAAGGAACACTTGATTGGCTCTACTTCTTAAACTGTTTGTAGAAGAGAACTTCACTTAAAATCACCTTTGTGAAAAGCATCCTGAACTCCTATTAATATCTTGGGATTTATGCCATATGGATTTTTTTTTCTTTGATATTCACAAGTATTTGGGCCAGCTTAACTATTCTTATTAACTATCCAAGCCTATTACATTTGGTTGCCAAGAAGGCAGATTGACTTTTGAAAAGGAAATAATTTCCTTATAAATTGAATCCCTTATGTTTTCTAAATGCTGCAGGCACCAGATAGATTTGTGGCAGCTGCTGCAAGGAATCCTGTGTGTAACCTTGCTTTAATGGTCGGCACATCAGATATTCCTGATTGGTGTTATGTGGAATGTTATGGAAGGGAAGGAAAAAATTACTTCACGGAAGCACCATCTGCTGAGCACCTAATCCACTTATACCACATGTCTCCTATTCGACATGTCTCAAATGTACGTATGATCAAAATCTAGCCATCTATAACTTTATTGATACAGCGATGTTTTAAAGTACTCGCTAAGGTTGGAAGAAAATAGTACTCATAAAAGCTATGGTCAACAGTTTTATAATCACATTAAGTCGAGATAATCAAATGGTACATATGAATAAATATTAAAATTCTTACTAAAGTTGAACAAAATAAAACTCATGTGGCTAGATTTTGCCCAACAGTTCAAATTGTATGTATCGCTAGATTTTGTTTATATATTATTGAAAAAGGAGAAAATGTAAGCTCTGAGGGCTTCATTGAAGAAGCCTCCTTCGTTGAAGGGCTTCACCCTGCATCAATATCAATTAAGGCTCTTCTAAGGTGAGGAGGATCCCTGTCTTGTGAGTGACTATTTTTTTTCTCTTTTTTTCTAAATGTGGCGAGATTTGTTATAAACTAAACTTGGAAAAATCCTAATCGGAAGATAACTTTTCTTTAGTTAAAATATCTTTGTCTGGAGAGTTAAATATTTTTATCTGGAGAATTATTTTACCAACTACTCTGTTTTGGAATTTTCTTTTTCCTTTTTAAAACAATAGTTTGAAAGTTATCCTGTATATTTATACTCTTAGTTTCACATATTTTCCGAGGATGTGTTAAGACTTTCTTTTGAGTAGATTTTCTCCGTTGAAATCTCATTTTGATTTTGCAGGTTAAAACACCTACCATTTTTCTTTTGGGTGCTAAAGATCTTCGTGTTCCATTTTCAAATGGATTGCAAGTAAGTTATAACACATTCTACTAAATTCTACATCCTTTTGATTTATTTGTGTGGCTATAAAATGGATACTGCGTTGCTCTTGTTTATAACCAAGAATGATTGCACAATGACATGAAATTTGCGTCCAATTTCTGGCTCTTTGAATTTCTGATAGTTACGTAAAAAAAAAAAAAAAAAAAAAAAAACTATATGATGGGCTATATATAATATGCAGTTTGCGCGAGCGTTGAAGGAGAAAGGAGTTGATACGAAGGTGATCGTGTTTCCAGATGATAAGCATCCAATTGACAGGTCCTACATTTGCCTGCTTTCACATTCACATATTCCGAACTCATTTTATGAATAGTTTCTGATTCCACATTTGTTTGGATCATTTGTTATAAGTTTAAGATTATAGTAACACTTCGACTGGTTTATGATTGTGAGGAAAGAGCACTTTCAATTACAAGATTTGAAAAATAGGAAACTATTTTAGGCTTATTCATTTTCTCTCTGAATCAGGCCGCAATCGGATTTTGAAAGCTTCCTCAACATTTGTGTGTGGTTCAAGAAGTACCTCAAGTGAGTGGCATTCCCTTTCTCAGCATTTTAGCATCATTGATCCCATGCTTTGTACTTATTTTGTCACTCTCAACTTGGTACAACTGGTTCTGTGCACTCTTGTTCATGTTATTAAGCCACTCATTCTCAATTAAATGACGCTTATTATTCCAACCTTTGAGCCTTACTTGCTCCTCACAAATTGATTGTTTGATTTTTGCAAGCAAAAATGTACAATGAGAAATGATAAATACCAAATAGTACTTGCAAGAGAAGAACTGAGC

mRNA sequence

TAATCTGATGGCGTTGGCTAGCACTTACTCCATTGGTCGGATCAACTTCCTTCGTCTTCCCCTCTTCGCCACTGCTGCTACTGCTCCTCATTCTCAGAGCTCTCAATTTCTCAGAGCCACCTGCCTCTTCTCTAGAAAACTGTCAACTTCCTTGGCCATGGACGGTTCAAAGATAAGTGCAGTTGTTGAGGAGTTTCCATCTGGGATTGATCCAGCAACTGAGGAAGAATATGTAGAACAGTCCAAATTACTTGAAGAATTCACAAAAATTCCTAACTTTGATAAGGCATGGACGTTTAAGTCTGATAGTGGAGACTCCGTGGTAACGTTTTCAATTAGCCAAGCAAATCTTCTAGCAAATAAGAAGAGAAAATATACTTTGTCAGCTCACATTTCCGAGGGAAATGACGACAGTGAAGTAAACTTTAAATGGACGCCATTCCCTGTTGAAATGGTTGGAATCTCTACAATAGTGCCATCGCCAGCAGGCTCAAAATTTCTTGCAGTTCGTAATCCTGAAAATGATTCTCCAGTTCAATTGGAAATTTGGAGTGCAGGTCAACTTGAAAAGGAGTTTCACATCCCCCAATCTGTTCATGGCTCAATATATACTGATGGATGGTTCGAGGGAATCTCTTGGAACTCAAATGAAACTTACATTGCTTATATTGCGGAGGAACCTTCTCCCTCCAAACCAACGTTTACTCTTTCTGGATATCAAAAAGGTTGCTCAACAGACAAGGATTCTACCAATTGGAAAGGTCAAGGGGATTTTAAGGAAGAATGGGGGGAAGCTTATGCAGGTAAAAGGCAGCCTGCTCTCTTTGTCGTCAATATTGACAGTGGAGAGGTAAAGCATGTGAAAATAAGCGAGCATTCTTTGAGTTTGGGTCAAGTTGTATGGGCTCCATCTATTGGCGAGCATCAATATTTGGTTTTTGTTGGATGGTCACCAGAGCCTCGAAAACTTGGTATGATCTACTGTTATAATCGGCCTTGTGCATTATATGCAGTTAAGGCCCCGGAGTTTGGATCAGAAGTCAATGAACATAAACTCAAAGATGAATCAAAAGAGGATTTTCCCATCTATAATCTAACTCAGAACATAAGCAGTGCCTTTTCTCCTCGGTTCAGCCCAGATGGAAAGTCACTTGTATTTTTATCTGCACACTCTTCTGTGAACACTGGCGCCCATTCCGCAACAAATTCTCTTCACAGAATCGACTGGCCTAGAGATGGGAAACTTAGTTTTAGTGAAACTATTGTTGATGTGGTTCCTGTGGTGCACTGGGCCAAGGACGACCTCTTTCCTGGCCTCTATTCACATGGTTTGCTGGCTAATCCATGCGGTGAAGTCTCATGCGTCAGTCCTGACCACACAGACTATTCTTGGAATGTTCTTGCTCTAGATGGAGACAACGTTATTGCTGTTCGTAGCGGCCCAGTGACTATTCCTGGACTCAAGTATGGTTTTCTTATTGAGACAGAAACTAAGGATGTTGTATGGAATTGGGTCGATGTATTAAATCCTATGTTTAATTGCTCTACGAAGGCTAGAACTCTACTCTCAAATATCTTGTTCAGTATATTGAAGATTCCAGTCAAGGATGTTTCCAACTGCGTGACAAAAGGTGCTAGTGAACCTTTTGAAGCTATCTTTGTAGCTCCGTTACCCAAAGGTAATGAGTTGAAACCACTTATTGTTAACCTTCATGGCGGTCCACATTCTACATCAACATCAAGCTTCTCGAAGTCCTTGGCATTTCTCGCTGCCGTAGGATTCAATCTTTTGATTGTGAACTATAGAGGTTCATTGGGGTTCGGTGAGGATGCATTGCAATCTCTTCCTGGAAAAATTGGGTCTCAGGATGTCAATGATGTACTTGCCGCATTAGATCACATTATCGACCAGGGACTTGCCAATCCAGCTAAAGTTGCAGTTCTTGGCGGTTCTCATGGAGGTTTTTTGACAACTCATTTGATTGGCCAGGCACCAGATAGATTTGTGGCAGCTGCTGCAAGGAATCCTGTGTGTAACCTTGCTTTAATGGTCGGCACATCAGATATTCCTGATTGGTGTTATGTGGAATGTTATGGAAGGGAAGGAAAAAATTACTTCACGGAAGCACCATCTGCTGAGCACCTAATCCACTTATACCACATGTCTCCTATTCGACATGTCTCAAATGTTAAAACACCTACCATTTTTCTTTTGGGTGCTAAAGATCTTCGTGTTCCATTTTCAAATGGATTGCAATTTGCGCGAGCGTTGAAGGAGAAAGGAGTTGATACGAAGGTGATCGTGTTTCCAGATGATAAGCATCCAATTGACAGGCCGCAATCGGATTTTGAAAGCTTCCTCAACATTTGTGTGTGGTTCAAGAAGTACCTCAAGTGAGTGGCATTCCCTTTCTCAGCATTTTAGCATCATTGATCCCATGCTTTGTACTTATTTTGTCACTCTCAACTTGGTACAACTGGTTCTGTGCACTCTTGTTCATGTTATTAAGCCACTCATTCTCAATTAAATGACGCTTATTATTCCAACCTTTGAGCCTTACTTGCTCCTCACAAATTGATTGTTTGATTTTTGCAAGCAAAAATGTACAATGAGAAATGATAAATACCAAATAGTACTTGCAAGAGAAGAACTGAGC

Coding sequence (CDS)

ATGGCGTTGGCTAGCACTTACTCCATTGGTCGGATCAACTTCCTTCGTCTTCCCCTCTTCGCCACTGCTGCTACTGCTCCTCATTCTCAGAGCTCTCAATTTCTCAGAGCCACCTGCCTCTTCTCTAGAAAACTGTCAACTTCCTTGGCCATGGACGGTTCAAAGATAAGTGCAGTTGTTGAGGAGTTTCCATCTGGGATTGATCCAGCAACTGAGGAAGAATATGTAGAACAGTCCAAATTACTTGAAGAATTCACAAAAATTCCTAACTTTGATAAGGCATGGACGTTTAAGTCTGATAGTGGAGACTCCGTGGTAACGTTTTCAATTAGCCAAGCAAATCTTCTAGCAAATAAGAAGAGAAAATATACTTTGTCAGCTCACATTTCCGAGGGAAATGACGACAGTGAAGTAAACTTTAAATGGACGCCATTCCCTGTTGAAATGGTTGGAATCTCTACAATAGTGCCATCGCCAGCAGGCTCAAAATTTCTTGCAGTTCGTAATCCTGAAAATGATTCTCCAGTTCAATTGGAAATTTGGAGTGCAGGTCAACTTGAAAAGGAGTTTCACATCCCCCAATCTGTTCATGGCTCAATATATACTGATGGATGGTTCGAGGGAATCTCTTGGAACTCAAATGAAACTTACATTGCTTATATTGCGGAGGAACCTTCTCCCTCCAAACCAACGTTTACTCTTTCTGGATATCAAAAAGGTTGCTCAACAGACAAGGATTCTACCAATTGGAAAGGTCAAGGGGATTTTAAGGAAGAATGGGGGGAAGCTTATGCAGGTAAAAGGCAGCCTGCTCTCTTTGTCGTCAATATTGACAGTGGAGAGGTAAAGCATGTGAAAATAAGCGAGCATTCTTTGAGTTTGGGTCAAGTTGTATGGGCTCCATCTATTGGCGAGCATCAATATTTGGTTTTTGTTGGATGGTCACCAGAGCCTCGAAAACTTGGTATGATCTACTGTTATAATCGGCCTTGTGCATTATATGCAGTTAAGGCCCCGGAGTTTGGATCAGAAGTCAATGAACATAAACTCAAAGATGAATCAAAAGAGGATTTTCCCATCTATAATCTAACTCAGAACATAAGCAGTGCCTTTTCTCCTCGGTTCAGCCCAGATGGAAAGTCACTTGTATTTTTATCTGCACACTCTTCTGTGAACACTGGCGCCCATTCCGCAACAAATTCTCTTCACAGAATCGACTGGCCTAGAGATGGGAAACTTAGTTTTAGTGAAACTATTGTTGATGTGGTTCCTGTGGTGCACTGGGCCAAGGACGACCTCTTTCCTGGCCTCTATTCACATGGTTTGCTGGCTAATCCATGCGGTGAAGTCTCATGCGTCAGTCCTGACCACACAGACTATTCTTGGAATGTTCTTGCTCTAGATGGAGACAACGTTATTGCTGTTCGTAGCGGCCCAGTGACTATTCCTGGACTCAAGTATGGTTTTCTTATTGAGACAGAAACTAAGGATGTTGTATGGAATTGGGTCGATGTATTAAATCCTATGTTTAATTGCTCTACGAAGGCTAGAACTCTACTCTCAAATATCTTGTTCAGTATATTGAAGATTCCAGTCAAGGATGTTTCCAACTGCGTGACAAAAGGTGCTAGTGAACCTTTTGAAGCTATCTTTGTAGCTCCGTTACCCAAAGGTAATGAGTTGAAACCACTTATTGTTAACCTTCATGGCGGTCCACATTCTACATCAACATCAAGCTTCTCGAAGTCCTTGGCATTTCTCGCTGCCGTAGGATTCAATCTTTTGATTGTGAACTATAGAGGTTCATTGGGGTTCGGTGAGGATGCATTGCAATCTCTTCCTGGAAAAATTGGGTCTCAGGATGTCAATGATGTACTTGCCGCATTAGATCACATTATCGACCAGGGACTTGCCAATCCAGCTAAAGTTGCAGTTCTTGGCGGTTCTCATGGAGGTTTTTTGACAACTCATTTGATTGGCCAGGCACCAGATAGATTTGTGGCAGCTGCTGCAAGGAATCCTGTGTGTAACCTTGCTTTAATGGTCGGCACATCAGATATTCCTGATTGGTGTTATGTGGAATGTTATGGAAGGGAAGGAAAAAATTACTTCACGGAAGCACCATCTGCTGAGCACCTAATCCACTTATACCACATGTCTCCTATTCGACATGTCTCAAATGTTAAAACACCTACCATTTTTCTTTTGGGTGCTAAAGATCTTCGTGTTCCATTTTCAAATGGATTGCAATTTGCGCGAGCGTTGAAGGAGAAAGGAGTTGATACGAAGGTGATCGTGTTTCCAGATGATAAGCATCCAATTGACAGGCCGCAATCGGATTTTGAAAGCTTCCTCAACATTTGTGTGTGGTTCAAGAAGTACCTCAAGTGA

Protein sequence

MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Homology
BLAST of Carg10061 vs. NCBI nr
Match: KAG7013512.1 (Acylamino-acid-releasing enzyme [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1635.5 bits (4234), Expect = 0.0e+00
Identity = 796/796 (100.00%), Postives = 796/796 (100.00%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
           MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60

Query: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
           EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120

Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
           RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180

Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
           WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240

Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
           CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300

Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
           PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360

Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
           YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420

Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV 480
           DVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV
Sbjct: 421 DVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV 480

Query: 481 TIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVT 540
           TIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVT
Sbjct: 481 TIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVT 540

Query: 541 KGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYR 600
           KGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYR
Sbjct: 541 KGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYR 600

Query: 601 GSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIG 660
           GSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIG
Sbjct: 601 GSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIG 660

Query: 661 QAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMS 720
           QAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMS
Sbjct: 661 QAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMS 720

Query: 721 PIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSD 780
           PIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSD
Sbjct: 721 PIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSD 780

Query: 781 FESFLNICVWFKKYLK 797
           FESFLNICVWFKKYLK
Sbjct: 781 FESFLNICVWFKKYLK 796

BLAST of Carg10061 vs. NCBI nr
Match: XP_022959045.1 (acylamino-acid-releasing enzyme isoform X1 [Cucurbita moschata])

HSP 1 Score: 1615.9 bits (4183), Expect = 0.0e+00
Identity = 794/822 (96.59%), Postives = 795/822 (96.72%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
           MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60

Query: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
           EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120

Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
           RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180

Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
           WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240

Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
           CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300

Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
           PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360

Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
           YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420

Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
           DVVPVVHWA+DDLFPGLYSHGLLANP                           GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSCVS 480

Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
           PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540

Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
           KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600

Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
           TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660

Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
           QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720

Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
           CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780

Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822

BLAST of Carg10061 vs. NCBI nr
Match: KAG6574947.1 (Acylamino-acid-releasing enzyme, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1614.0 bits (4178), Expect = 0.0e+00
Identity = 793/822 (96.47%), Postives = 794/822 (96.59%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
           MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60

Query: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
           EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120

Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
           RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180

Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
           WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240

Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
           CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300

Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
           PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360

Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
           YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSE IV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSENIV 420

Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
           DVVPVVHWA+DDLFPGLYSHGLLANP                           GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSCVS 480

Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
           PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540

Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
           KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600

Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
           TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660

Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
           QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720

Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
           CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780

Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822

BLAST of Carg10061 vs. NCBI nr
Match: XP_023548261.1 (acylamino-acid-releasing enzyme isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 785/822 (95.50%), Postives = 792/822 (96.35%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
           MALA TYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLST+LAMDGSKISAVV
Sbjct: 1   MALAGTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTALAMDGSKISAVV 60

Query: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
           EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120

Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
           RKYTLSAHISEGNDDSEVNFKWTPFPVEM+GISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMIGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180

Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
           WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240

Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
           CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEHSLSLGQVVWA 300

Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
           PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360

Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
           YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSE IV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSENIV 420

Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
           DVVPVVHWA+DDLFPGLYSHGLLANP                           GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIITTTIWRSRSAIVSIDILSGEVSCVS 480

Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
           PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540

Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
           KARTLLSNILFSILKIPVKDVSNCVTKGA+EPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGATEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600

Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
           TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660

Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
           QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720

Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
           CYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780

Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           LKEKGV+TKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVETKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822

BLAST of Carg10061 vs. NCBI nr
Match: XP_023006699.1 (acylamino-acid-releasing enzyme isoform X1 [Cucurbita maxima])

HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 775/824 (94.05%), Postives = 785/824 (95.27%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAAT--APHSQSSQFLRATCLFSRKLSTSLAMDGSKISA 60
           MALA TYSIGRINFLRLPLF TA T  APHSQSSQFLRATCLFSRKLST+LAMDGSKISA
Sbjct: 1   MALAGTYSIGRINFLRLPLFVTAVTAPAPHSQSSQFLRATCLFSRKLSTALAMDGSKISA 60

Query: 61  VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120
           VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN
Sbjct: 61  VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120

Query: 121 KKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQL 180
           KKRKYTLSAHIS GNDDSEVNFKWTPFPVEM+G+STIVPSPAGSKFLAVRNPENDSPVQL
Sbjct: 121 KKRKYTLSAHISNGNDDSEVNFKWTPFPVEMIGVSTIVPSPAGSKFLAVRNPENDSPVQL 180

Query: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240
           EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ
Sbjct: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240

Query: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVV 300
           KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EHSLSLGQVV
Sbjct: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEHSLSLGQVV 300

Query: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDF 360
           WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDE+KEDF
Sbjct: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDEAKEDF 360

Query: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSET 420
           PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAH ATNSLHRIDWPRD KLSFSE 
Sbjct: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHCATNSLHRIDWPRDEKLSFSEN 420

Query: 421 IVDVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSC 480
           IVDVVPVVHWA+DDLFPGLYSHGLLANP                           GEVSC
Sbjct: 421 IVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSC 480

Query: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540
           VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC
Sbjct: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540

Query: 541 STKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGP 600
           STKARTLLS+ILFSILKIPVKDVSNCVTKGASEPFEAIFV+P  KGNELKPLIVNLHGGP
Sbjct: 541 STKARTLLSHILFSILKIPVKDVSNCVTKGASEPFEAIFVSPFSKGNELKPLIVNLHGGP 600

Query: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660
           HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI
Sbjct: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660

Query: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720
           IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY
Sbjct: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720

Query: 721 VECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780
           VECYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA
Sbjct: 721 VECYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780

Query: 781 RALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           RALKEKGVDTKVI+FPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 RALKEKGVDTKVIMFPDDKHPIDRPQSDFESFLNICVWFKKYLK 824

BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match: Q84LM4 (Acylamino-acid-releasing enzyme OS=Arabidopsis thaliana OX=3702 GN=AARE PE=1 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 5.9e-266
Identity = 453/776 (58.38%), Postives = 567/776 (73.07%), Query Frame = 0

Query: 51  MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
           MD S   +  +E   G+DP TEEEY  QSKLL+EF  IP+ DKAW F SDSG S   F++
Sbjct: 1   MDSSGTDS-AKELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSG-SQAMFAL 60

Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
           SQANLLANKK+K+ LS HIS  ++ S VNF W PFP+EM G S  VPSP+G K L +RNP
Sbjct: 61  SQANLLANKKKKFMLSGHISNESNQS-VNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNP 120

Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
           EN+SP + EIW++ QLEKEFHIPQ VHGS+Y DGWFEGISW+S+ET++AY+AEEPS  KP
Sbjct: 121 ENESPTKFEIWNSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKP 180

Query: 231 TFT-LSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISE 290
           TF  L  Y+K  S DK   +WKG+GD++EEWGEAYAGKRQPALFV+N+DSGEV+ +K   
Sbjct: 181 TFDHLGYYKKENSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIP 240

Query: 291 HSLSLGQVVWAP-SIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEH 350
            S+S+GQVVW+P S G  QYLVF GW  + RK G+ YCYNRPCA+YA+K   F S+    
Sbjct: 241 RSISVGQVVWSPNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIK---FTSD---- 300

Query: 351 KLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWP 410
           + KD+   +FPI+NLT++ISS F PRFS DGK LVF+SA ++V++GAH AT SLHRIDWP
Sbjct: 301 EPKDDDANEFPIHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWP 360

Query: 411 RDGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP---------------------- 470
            DGKL  S  IVDV+ VV+  KD  FPGLY  GLL++P                      
Sbjct: 361 SDGKLPESTNIVDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILS 420

Query: 471 ----CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNW 530
                GEVS  SP  +DYSWN LALDGD+++AV S PV++P +KYG           W W
Sbjct: 421 VNLLSGEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLW 480

Query: 531 VDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFV--APLPKGNE 590
            ++ +P+   S K    LS++ F ILK+P+ DVS  + +GA  P EAI+V  +   +  +
Sbjct: 481 SNIQSPI-RYSEKVMAGLSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGK 540

Query: 591 LKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQ 650
             PLI  LHGGPHS S  SFS+++A+L+++G++ LI+NYRGSLG+GEDALQSLPGK+GSQ
Sbjct: 541 CDPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQ 600

Query: 651 DVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLAL 710
           DV D L A+DH I+ G+A+P+++ VLGGSHGGFLTTHLIGQAPD+FVAAAARNPVCN+A 
Sbjct: 601 DVKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMAS 660

Query: 711 MVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKD 770
           MVG +DIPDWC+ E YG   ++++TEAPSAE L   + MSPI H+S VKTPT+FLLG KD
Sbjct: 661 MVGITDIPDWCFFEAYG--DQSHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKD 720

Query: 771 LRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           LRVP SNG Q+ RALKEKGV+ KV+VFP+D HP+DRPQ+D+ESFLNI VWF KY K
Sbjct: 721 LRVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDRPQTDYESFLNIAVWFNKYCK 763

BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match: Q0IXP9 (Acylamino-acid-releasing enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0415600 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 1.3e-249
Identity = 433/772 (56.09%), Postives = 542/772 (70.21%), Query Frame = 0

Query: 57  SAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDS-GDSVVTFSISQANL 116
           +A  +  P G+D +  +EY  QSKLL+EF KIP    AW F S +   S    S+ Q +L
Sbjct: 9   AATEKGLPLGMDVSMVDEYASQSKLLQEFVKIPTIGNAWIFNSKTENTSRAIVSVGQTDL 68

Query: 117 LANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSP 176
           LANKKR + L++HIS+ N  + V+F+W+PFP+EM G+S ++PSP+G K L +RN E+DSP
Sbjct: 69  LANKKRSFLLNSHISK-NSSNSVDFQWSPFPIEMSGVSAVIPSPSGRKLLLIRNSEDDSP 128

Query: 177 VQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLS 236
            +LE+W   QLE E HI QSVHGS+Y D WFEGISWN  ET +AY+AEEP   KP F  S
Sbjct: 129 TKLEVWGPCQLENEIHIAQSVHGSLYVDEWFEGISWNQEETLVAYVAEEPPQPKPEFNDS 188

Query: 237 GYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLG 296
           GY+K  S++KD  +WKG+GD++E WGE Y+ KR PALFVVNI SGEV+ VK    +LS+G
Sbjct: 189 GYKKAGSSEKDCKSWKGKGDWEETWGETYSKKRIPALFVVNISSGEVRAVKGIPRTLSVG 248

Query: 297 QVVWAPSIGEHQYLVFVGWSPE------PRKLGMIYCYNRPCALYAVKAPEFGSEVNEHK 356
           QV+WAPS      LVFV WS +      PRKLG+ YC+NRPCALYAV  P F  E ++  
Sbjct: 249 QVIWAPS--SSHSLVFVAWSSDNGYQKTPRKLGIKYCFNRPCALYAVPDP-FMEEADKPS 308

Query: 357 LKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPR 416
           L     E  P   LT  +SSAF PRFSPDGK LVF+SA S++++G H+ATNS+H+IDWP 
Sbjct: 309 LNVSKGETAPTTKLTSELSSAFFPRFSPDGKYLVFISAKSAIDSGTHNATNSMHKIDWPA 368

Query: 417 DGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP----------------------- 476
           DGKL    ++ DVVP+V   +D  FPGLY  G+L NP                       
Sbjct: 369 DGKLE-GLSVADVVPIVMCPQDGCFPGLYCSGILRNPWLTDGQTMILSSIWGSKEVILSV 428

Query: 477 ---CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWV 536
                EVS VSP  +DYSWNVLALD DN++AV S  +T+P + YG  +    K   W W 
Sbjct: 429 NVVSREVSRVSPQDSDYSWNVLALDKDNILAVSSSLITVPQIYYGSEVCQTGKPNQWEWQ 488

Query: 537 DVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKP 596
           ++  P  + S K   +L++  FSILKIP+ + SN +  GA  PFEAIFV+   K +  +P
Sbjct: 489 EIATPFPSPSDKISAILADHKFSILKIPISNSSNKLADGAKLPFEAIFVS--WKDSATRP 548

Query: 597 LIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVN 656
            IV LHGGPH+   SS+SKSLAFL + G+NLL+VNYRGSLGFGE+ALQSLPG IGSQDVN
Sbjct: 549 TIVVLHGGPHTVYPSSYSKSLAFLYSQGYNLLVVNYRGSLGFGEEALQSLPGNIGSQDVN 608

Query: 657 DVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVG 716
           DVL ALD +I +GL + +KVAV+GGSHGGFLTTHLIGQAP  FVAAAARNPVCNL+LMVG
Sbjct: 609 DVLTALDFVIKKGLIDASKVAVVGGSHGGFLTTHLIGQAPGTFVAAAARNPVCNLSLMVG 668

Query: 717 TSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRV 776
           T+DIP+WC+VE YG+EGKN F+E PS + L   +  SPI H+S V TPT+FLLGA+DLRV
Sbjct: 669 TTDIPEWCFVEIYGKEGKNCFSEYPSFDDLCQFHQKSPISHISKVSTPTLFLLGAQDLRV 728

Query: 777 PFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYL 796
           P SNGLQ+AR LKE GV+TK+IVFP+D H +D+PQSDFESFLNI VWFKK++
Sbjct: 729 PVSNGLQYARTLKEMGVETKIIVFPEDMHGLDKPQSDFESFLNIGVWFKKHM 773

BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match: Q338C0 (Acylamino-acid-releasing enzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0415800 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.9e-236
Identity = 410/766 (53.52%), Postives = 534/766 (69.71%), Query Frame = 0

Query: 67  IDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGD-SVVTFSISQANLLANKKRKYTL 126
           +D    EEY  QSKLL+EFT  P+ D AW F++++ D S   +SISQ NLLAN KRKY L
Sbjct: 1   MDALASEEYASQSKLLQEFTNAPSIDGAWVFQTNNEDRSTAMYSISQTNLLANNKRKYIL 60

Query: 127 SAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEIWSAGQ 186
            +HI   N  + ++F+W+PFP++M G+S +VPSP+GSK L VRN E  SP +LEI     
Sbjct: 61  FSHIMR-NGTNLLDFQWSPFPIQMDGVSAVVPSPSGSKLLVVRNGEKGSPTKLEIVDQSH 120

Query: 187 LEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKGCSTDK 246
           +EKE H+ QSVHG +YTD WF GISWN  ET IAYIAE+    KP F  +GY+K  S++K
Sbjct: 121 VEKEIHVAQSVHGPLYTDEWFHGISWNQEETLIAYIAEDSPEPKPVFDDTGYRKEGSSEK 180

Query: 247 DSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWAP--SI 306
           D  NWKGQGD++E+WGE Y+ K +P+LFV++I+SGEV+  K    SLS+GQVVWAP  S 
Sbjct: 181 DCNNWKGQGDWEEDWGETYSKKGRPSLFVLDINSGEVRAAKGISRSLSVGQVVWAPPSSC 240

Query: 307 GEHQYLVFVGW------SPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKED 366
           G  +YL+FVGW         PRKLG+ YC NRPC+LY+   P   S+V+     D   E 
Sbjct: 241 GRQKYLIFVGWLEHNGFQNTPRKLGIKYCSNRPCSLYSTLCPFEESDVDNAPASDSKLEP 300

Query: 367 FPI-YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFS 426
             +  NLT +ISSAF PRFS DGK LVFLSA+ +V++GAH+AT+SLH+I+WP D K+   
Sbjct: 301 ASVAINLTPSISSAFFPRFSKDGKLLVFLSANRAVDSGAHNATDSLHKINWPSDWKMDQY 360

Query: 427 ETIVDVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEV 486
             I DV+P+V   +D  FPGLY   +L+NP                           G+ 
Sbjct: 361 LEITDVIPIVMCPQDGCFPGLYCSSMLSNPWLSDRCTMILTSAWRSTEVILSIDVLSGKA 420

Query: 487 SCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDV-LNPM 546
           + +SP++++YSW+ LA+DG NV+AV S P+  P +KYG  +  + +   W W +V  NP+
Sbjct: 421 TRISPENSEYSWSALAVDGHNVLAVSSSPIDPPQIKYGHQVSLKDQTCTWVWDEVNNNPL 480

Query: 547 FNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLH 606
              + K + LLS+  FSILKIPV + S+ ++ G+  PFEAIFV+   K +  KP I+ LH
Sbjct: 481 MAANNKVKALLSHHQFSILKIPVTNPSDDLSDGSKLPFEAIFVS--CKDSSHKPTILVLH 540

Query: 607 GGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAAL 666
           GGPHS S SS+SK+ AFLA++GFNLLIVNYRG+ GFGE+ALQSLPGK+GSQDV D L AL
Sbjct: 541 GGPHSVSVSSYSKTSAFLASLGFNLLIVNYRGTPGFGEEALQSLPGKVGSQDVQDCLTAL 600

Query: 667 DHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPD 726
           D++I+ GL + +KVAV+G SHGGFLTTHLIGQAPDRF+ AAARNPVCNL+LM+GT+DIPD
Sbjct: 601 DYVIEGGLIDASKVAVIGISHGGFLTTHLIGQAPDRFMVAAARNPVCNLSLMIGTTDIPD 660

Query: 727 WCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGL 786
           WCY    G EG+ + +E+PS +HL   Y  SPI H+S VK P + LLG  DLRVP SNGL
Sbjct: 661 WCYAVACGSEGRQHASESPSPDHLRLFYQKSPIAHISKVKAPLLMLLGGADLRVPISNGL 720

Query: 787 QFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYL 796
           Q+ARAL+E+G + ++++FPDD H I+ PQSDFESFLNI VWFKK+L
Sbjct: 721 QYARALRERGGEIRIMMFPDDIHEINIPQSDFESFLNIGVWFKKHL 763

BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match: Q8R146 (Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3)

HSP 1 Score: 335.9 bits (860), Expect = 1.3e-90
Identity = 215/648 (33.18%), Postives = 326/648 (50.31%), Query Frame = 0

Query: 178 LEIWSAGQLEKEFHIPQ-SVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSG 237
           LE+W   +  K F++     HG +Y D  F  +SW+ +ET++ Y+AE+  P   +F  + 
Sbjct: 121 LEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTK 180

Query: 238 YQKGCSTDKDSTNWKG-----QGD---FKEEWGEAYAGKRQPALFVVNIDSGEVKHVKIS 297
                ++D++    K      +GD   F E+WGE    K  P L V++I+SG +  ++  
Sbjct: 181 ALDVSASDEEMARPKKPDQAIKGDQFVFYEDWGETMVSKSIPVLCVLDIESGNISVLEGV 240

Query: 298 EHSLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEH 357
             ++S GQ  WAP       +VFVGW  EP +LG+ YC NR  ALY V       E+   
Sbjct: 241 PENVSPGQAFWAPG---DTGVVFVGWWHEPFRLGIRYCTNRRSALYYVDLSGGKCEL--- 300

Query: 358 KLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDW- 417
            L DES              +  SPR SPD   +V+L   S      H   + L   DW 
Sbjct: 301 -LSDES-------------LAVCSPRLSPDQCRVVYLQYPS---LAPHHQCSQLFLYDWY 360

Query: 418 -----------PRDGKLSFSETIVDVVPVVHWAKDD---LFPGLY--SHGLLA--NPCGE 477
                      PR    SFS     ++P+  W+ D    +F  +      L A     G 
Sbjct: 361 TKVTSLVVDIVPRQLGESFSGIYCSLLPLGCWSADSQRVVFDSVQRSRQDLFAVDTQTGS 420

Query: 478 VSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIET-ETKDVVWNWVDVLNP 537
           V+ ++   +  SW +L +D D ++A  S P   P LK GFL    + + V W  ++   P
Sbjct: 421 VTSLTAGGSAGSWKLLTIDRDLMVAQFSTPNLPPSLKVGFLPPAGKEQSVSWVSLEEAEP 480

Query: 538 MFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELK-PLIVN 597
           + +     R L           P  D  N   + A   FEAI + P    ++ + P++V 
Sbjct: 481 IPDIHWGIRVL----------HPPPDQEN--VQYADLDFEAILLQPSNSPDKSQVPMVVM 540

Query: 598 LHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLA 657
            HGGPHS+  +++    A L  +GF +L+VNYRGS GFG+D++ SLPG +G QDV DV  
Sbjct: 541 PHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQF 600

Query: 658 ALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDI 717
           A+  ++ +   +  +VA++GGSHGGFL+ HLIGQ P+ + A  ARNPV N+  M+GT+DI
Sbjct: 601 AVQQVLQEEHFDARRVALMGGSHGGFLSCHLIGQYPETYSACIARNPVINIVSMMGTTDI 660

Query: 718 PDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSN 777
           PDWC VE       +Y    P    L  +   SPI+++  VKTP + +LG +D RVPF  
Sbjct: 661 PDWCMVETGFPYSNDYL---PDLNVLEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQ 720

Query: 778 GLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYL 796
           GL++  ALK + V  +++++P   H +   + + +SF+N  +W   +L
Sbjct: 721 GLEYYHALKARNVPVRLLLYPKSTHALSEVEVESDSFMNTVLWLHTHL 730

BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match: P80227 (Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2)

HSP 1 Score: 335.1 bits (858), Expect = 2.1e-90
Identity = 221/678 (32.60%), Postives = 332/678 (48.97%), Query Frame = 0

Query: 158 SPAGSKFLAVRNPENDSPVQ--LEIWSAGQLEKEFHIPQ-SVHGSIYTDGWFEGISWNSN 217
           SP+G+    +R   +    +  LE+W   +  K F++     HG +Y D  F  +SW+ +
Sbjct: 97  SPSGTMKAVLRKAGSTGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHS 156

Query: 218 ETYIAYIAEEPSPSKPTF------TLSGYQKGCSTDK--DSTNWKGQGDFKEEWGEAYAG 277
           ET++ Y+AE+  P   +F       +SG     +  K  D      Q  F E+WGE    
Sbjct: 157 ETHLLYVAEKKRPKAESFFQTKALDISGSDDEMARPKKPDQAIKGDQFLFYEDWGENMVS 216

Query: 278 KRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYC 337
           K  P L V++I+SG +  ++    S+S GQ  WAP       +VF GW  EP +LG+ +C
Sbjct: 217 KGSPVLCVLDIESGNISVLEGVPESVSPGQAFWAPG---DTGVVFAGWWHEPFRLGIRFC 276

Query: 338 YNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLS 397
            NR  ALY V       E+    L D+S              +  SPR SPD   +V+L 
Sbjct: 277 TNRRSALYYVDLTGGNCEL----LSDDS-------------LAVTSPRLSPDQCRIVYLQ 336

Query: 398 AHSSVNTGAHSATNSLHRIDW------------PRDGKLSFSETIVDVVPVVHWAKD--- 457
             S V    H     L   DW            PR    +FS     ++P+  W+ D   
Sbjct: 337 FPSLV---PHQQCGQLCLYDWYTRVTVVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQR 396

Query: 458 -----------DLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV 517
                      DLF        +    G V+ ++   +  SW +L +D D ++A  S P 
Sbjct: 397 VVFDTAQRSRQDLF-------AVDTQMGTVTPLTAGGSGGSWKLLTIDRDLMVAQFSTPN 456

Query: 518 TIPGLKYGFLIET-ETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCV 577
             P LK GFL      ++VVW  ++   P+ + S   R L           P  +  +  
Sbjct: 457 LPPCLKVGFLPPAGMEQEVVWVSLEEAEPIPDISWSIRVLQ----------PPPEQEHAQ 516

Query: 578 TKGASEPFEAIFVAPL-PKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVN 637
             G    FEAI + P  P      P++V  HGGPHS+  +S+    A L  +GF  L+VN
Sbjct: 517 YVGLD--FEAILIQPSNPPDKTQVPMVVMPHGGPHSSFVTSWMLLPAMLCKMGFAALLVN 576

Query: 638 YRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHL 697
           YRGS GFG+D++ SLPG +GSQDV DV  A++ ++ +   +  +VA+LGGSHGGFL+ HL
Sbjct: 577 YRGSTGFGQDSILSLPGNVGSQDVKDVQFAVEQVLQEEHFDAGRVALLGGSHGGFLSCHL 636

Query: 698 IGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEA-PSAEHLIHLY 757
           IGQ P+ + A   RNPV N+A M+G++DIPDWC VE     G  Y ++  P       + 
Sbjct: 637 IGQYPETYGACVVRNPVINIASMMGSTDIPDWCVVEA----GYLYSSDCLPDPNVWSEML 696

Query: 758 HMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRP 796
           + SPI++   VKTP + +LG +D RVPF  G+++ RALK + V  +++++P   H +   
Sbjct: 697 NKSPIKYTPQVKTPVLLMLGQEDRRVPFKQGMEYYRALKARNVPVRLLLYPKSTHSLSEV 728

BLAST of Carg10061 vs. ExPASy TrEMBL
Match: A0A6J1H567 (acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460152 PE=4 SV=1)

HSP 1 Score: 1615.9 bits (4183), Expect = 0.0e+00
Identity = 794/822 (96.59%), Postives = 795/822 (96.72%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
           MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60

Query: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
           EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120

Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
           RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180

Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
           WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240

Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
           CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300

Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
           PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360

Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
           YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420

Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
           DVVPVVHWA+DDLFPGLYSHGLLANP                           GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSCVS 480

Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
           PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540

Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
           KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600

Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
           TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660

Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
           QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720

Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
           CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780

Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822

BLAST of Carg10061 vs. ExPASy TrEMBL
Match: A0A6J1L2W5 (acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499357 PE=4 SV=1)

HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 775/824 (94.05%), Postives = 785/824 (95.27%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAAT--APHSQSSQFLRATCLFSRKLSTSLAMDGSKISA 60
           MALA TYSIGRINFLRLPLF TA T  APHSQSSQFLRATCLFSRKLST+LAMDGSKISA
Sbjct: 1   MALAGTYSIGRINFLRLPLFVTAVTAPAPHSQSSQFLRATCLFSRKLSTALAMDGSKISA 60

Query: 61  VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120
           VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN
Sbjct: 61  VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120

Query: 121 KKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQL 180
           KKRKYTLSAHIS GNDDSEVNFKWTPFPVEM+G+STIVPSPAGSKFLAVRNPENDSPVQL
Sbjct: 121 KKRKYTLSAHISNGNDDSEVNFKWTPFPVEMIGVSTIVPSPAGSKFLAVRNPENDSPVQL 180

Query: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240
           EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ
Sbjct: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240

Query: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVV 300
           KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EHSLSLGQVV
Sbjct: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEHSLSLGQVV 300

Query: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDF 360
           WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDE+KEDF
Sbjct: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDEAKEDF 360

Query: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSET 420
           PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAH ATNSLHRIDWPRD KLSFSE 
Sbjct: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHCATNSLHRIDWPRDEKLSFSEN 420

Query: 421 IVDVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSC 480
           IVDVVPVVHWA+DDLFPGLYSHGLLANP                           GEVSC
Sbjct: 421 IVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSC 480

Query: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540
           VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC
Sbjct: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540

Query: 541 STKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGP 600
           STKARTLLS+ILFSILKIPVKDVSNCVTKGASEPFEAIFV+P  KGNELKPLIVNLHGGP
Sbjct: 541 STKARTLLSHILFSILKIPVKDVSNCVTKGASEPFEAIFVSPFSKGNELKPLIVNLHGGP 600

Query: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660
           HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI
Sbjct: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660

Query: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720
           IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY
Sbjct: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720

Query: 721 VECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780
           VECYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA
Sbjct: 721 VECYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780

Query: 781 RALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           RALKEKGVDTKVI+FPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 RALKEKGVDTKVIMFPDDKHPIDRPQSDFESFLNICVWFKKYLK 824

BLAST of Carg10061 vs. ExPASy TrEMBL
Match: A0A6J1H3S3 (acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460152 PE=4 SV=1)

HSP 1 Score: 1525.4 bits (3948), Expect = 0.0e+00
Identity = 744/772 (96.37%), Postives = 745/772 (96.50%), Query Frame = 0

Query: 51  MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
           MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI
Sbjct: 1   MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 60

Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
           SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP
Sbjct: 61  SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 120

Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
           ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP
Sbjct: 121 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 180

Query: 231 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH 290
           TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH
Sbjct: 181 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH 240

Query: 291 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 350
           SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL
Sbjct: 241 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 300

Query: 351 KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD 410
           KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD
Sbjct: 301 KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD 360

Query: 411 GKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP------------------------ 470
           GKLSFSETIVDVVPVVHWA+DDLFPGLYSHGLLANP                        
Sbjct: 361 GKLSFSETIVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSID 420

Query: 471 --CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 530
              GEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD
Sbjct: 421 ILSGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 480

Query: 531 VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL 590
           VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL
Sbjct: 481 VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL 540

Query: 591 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 650
           IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND
Sbjct: 541 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 600

Query: 651 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 710
           VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT
Sbjct: 601 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 660

Query: 711 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP 770
           SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP
Sbjct: 661 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP 720

Query: 771 FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 721 FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 772

BLAST of Carg10061 vs. ExPASy TrEMBL
Match: A0A6J1L0V8 (acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499357 PE=4 SV=1)

HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 729/772 (94.43%), Postives = 738/772 (95.60%), Query Frame = 0

Query: 51  MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
           MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI
Sbjct: 1   MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 60

Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
           SQANLLANKKRKYTLSAHIS GNDDSEVNFKWTPFPVEM+G+STIVPSPAGSKFLAVRNP
Sbjct: 61  SQANLLANKKRKYTLSAHISNGNDDSEVNFKWTPFPVEMIGVSTIVPSPAGSKFLAVRNP 120

Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
           ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP
Sbjct: 121 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 180

Query: 231 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH 290
           TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EH
Sbjct: 181 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEH 240

Query: 291 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 350
           SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL
Sbjct: 241 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 300

Query: 351 KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD 410
           KDE+KEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAH ATNSLHRIDWPRD
Sbjct: 301 KDEAKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHCATNSLHRIDWPRD 360

Query: 411 GKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP------------------------ 470
            KLSFSE IVDVVPVVHWA+DDLFPGLYSHGLLANP                        
Sbjct: 361 EKLSFSENIVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSID 420

Query: 471 --CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 530
              GEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD
Sbjct: 421 ILSGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 480

Query: 531 VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL 590
           VLNPMFNCSTKARTLLS+ILFSILKIPVKDVSNCVTKGASEPFEAIFV+P  KGNELKPL
Sbjct: 481 VLNPMFNCSTKARTLLSHILFSILKIPVKDVSNCVTKGASEPFEAIFVSPFSKGNELKPL 540

Query: 591 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 650
           IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND
Sbjct: 541 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 600

Query: 651 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 710
           VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT
Sbjct: 601 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 660

Query: 711 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP 770
           SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVP
Sbjct: 661 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVP 720

Query: 771 FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           FSNGLQFARALKEKGVDTKVI+FPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 721 FSNGLQFARALKEKGVDTKVIMFPDDKHPIDRPQSDFESFLNICVWFKKYLK 772

BLAST of Carg10061 vs. ExPASy TrEMBL
Match: A0A6J1CDB8 (acylamino-acid-releasing enzyme isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009690 PE=4 SV=1)

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 658/823 (79.95%), Postives = 720/823 (87.48%), Query Frame = 0

Query: 1   MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
           MAL     IGR++ LRLPLFA AATA  S+SS F RAT LF+RKLSTS AMDGSKISAVV
Sbjct: 1   MALTGIAPIGRVSLLRLPLFAIAATATPSRSSPFFRATSLFTRKLSTSFAMDGSKISAVV 60

Query: 61  EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
           +E P+G+DP TE EY EQSKLL+EFT IPN DKAW FKSDSGDSV TFSISQA+LL+NK+
Sbjct: 61  DELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSISQASLLSNKR 120

Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
           RKYTLSAHIS+GN DS VNFKW PFPVEM+G+STIVPSP+GSK L VRN ENDSPV+LEI
Sbjct: 121 RKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEI 180

Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
           WSAGQ+EKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFT+SGYQKG
Sbjct: 181 WSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSGYQKG 240

Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
           CST+KDSTNWK QGDF+EEWGEAYAGKRQPALFV+NIDSGEV+HVK+ E  LS+GQVVWA
Sbjct: 241 CSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWA 300

Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
           PSIG HQYLVF GWS EPRKLGM YCYNRPCALYAV+AP++GSEV+EHKLKD SKEDFP+
Sbjct: 301 PSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFPL 360

Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
           YNLTQNISSAF PRFSPDGK L+FLSA+SSVNTGAHSATNSLHRI+WP D KL F+E IV
Sbjct: 361 YNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCFNENIV 420

Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
           DVVPVVHWA+DDLFPGLYSHG L NP                           GEVS +S
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILSGEVSRIS 480

Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
           P ++++SWNVLALDGDNV+AV S PV IP LKYG LIE ETK+ VW+W+DV +P+F CS 
Sbjct: 481 PSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVWSWLDVSSPVFKCSE 540

Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLP-KGNELKPLIVNLHGGPH 600
           K +TLLS + FSI+KIPVKDVSNC+TKGAS+PFEAIFV+    KGNEL P+IVNLHGGPH
Sbjct: 541 KVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPH 600

Query: 601 STSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHII 660
           STS SSFSKS AFL+++GFNLL+VNYRGSLGFGEDALQSLPGK+GSQDVNDVLAALDHII
Sbjct: 601 STSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQDVNDVLAALDHII 660

Query: 661 DQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYV 720
           D+GLANP+KVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYV
Sbjct: 661 DKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYV 720

Query: 721 ECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFAR 780
           ECYGREGKNYFTEAPSAEH I LY+ SPI HVS VK PTIFLLGAKDLRVPFSNGLQFAR
Sbjct: 721 ECYGREGKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFAR 780

Query: 781 ALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           ALKEKGV+ KVI+FPDD HPIDRPQSDFESFLNI VWFKKY K
Sbjct: 781 ALKEKGVEVKVIMFPDDIHPIDRPQSDFESFLNIGVWFKKYCK 823

BLAST of Carg10061 vs. TAIR 10
Match: AT4G14570.1 (acylaminoacyl-peptidase-related )

HSP 1 Score: 918.3 bits (2372), Expect = 4.2e-267
Identity = 453/776 (58.38%), Postives = 567/776 (73.07%), Query Frame = 0

Query: 51  MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
           MD S   +  +E   G+DP TEEEY  QSKLL+EF  IP+ DKAW F SDSG S   F++
Sbjct: 1   MDSSGTDS-AKELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSG-SQAMFAL 60

Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
           SQANLLANKK+K+ LS HIS  ++ S VNF W PFP+EM G S  VPSP+G K L +RNP
Sbjct: 61  SQANLLANKKKKFMLSGHISNESNQS-VNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNP 120

Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
           EN+SP + EIW++ QLEKEFHIPQ VHGS+Y DGWFEGISW+S+ET++AY+AEEPS  KP
Sbjct: 121 ENESPTKFEIWNSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKP 180

Query: 231 TFT-LSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISE 290
           TF  L  Y+K  S DK   +WKG+GD++EEWGEAYAGKRQPALFV+N+DSGEV+ +K   
Sbjct: 181 TFDHLGYYKKENSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIP 240

Query: 291 HSLSLGQVVWAP-SIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEH 350
            S+S+GQVVW+P S G  QYLVF GW  + RK G+ YCYNRPCA+YA+K   F S+    
Sbjct: 241 RSISVGQVVWSPNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIK---FTSD---- 300

Query: 351 KLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWP 410
           + KD+   +FPI+NLT++ISS F PRFS DGK LVF+SA ++V++GAH AT SLHRIDWP
Sbjct: 301 EPKDDDANEFPIHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWP 360

Query: 411 RDGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP---------------------- 470
            DGKL  S  IVDV+ VV+  KD  FPGLY  GLL++P                      
Sbjct: 361 SDGKLPESTNIVDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILS 420

Query: 471 ----CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNW 530
                GEVS  SP  +DYSWN LALDGD+++AV S PV++P +KYG           W W
Sbjct: 421 VNLLSGEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLW 480

Query: 531 VDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFV--APLPKGNE 590
            ++ +P+   S K    LS++ F ILK+P+ DVS  + +GA  P EAI+V  +   +  +
Sbjct: 481 SNIQSPI-RYSEKVMAGLSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGK 540

Query: 591 LKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQ 650
             PLI  LHGGPHS S  SFS+++A+L+++G++ LI+NYRGSLG+GEDALQSLPGK+GSQ
Sbjct: 541 CDPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQ 600

Query: 651 DVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLAL 710
           DV D L A+DH I+ G+A+P+++ VLGGSHGGFLTTHLIGQAPD+FVAAAARNPVCN+A 
Sbjct: 601 DVKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMAS 660

Query: 711 MVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKD 770
           MVG +DIPDWC+ E YG   ++++TEAPSAE L   + MSPI H+S VKTPT+FLLG KD
Sbjct: 661 MVGITDIPDWCFFEAYG--DQSHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKD 720

Query: 771 LRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
           LRVP SNG Q+ RALKEKGV+ KV+VFP+D HP+DRPQ+D+ESFLNI VWF KY K
Sbjct: 721 LRVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDRPQTDYESFLNIAVWFNKYCK 763

BLAST of Carg10061 vs. TAIR 10
Match: AT5G36210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 85.1 bits (209), Expect = 2.8e-16
Identity = 55/213 (25.82%), Postives = 97/213 (45.54%), Query Frame = 0

Query: 560 ELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGS 619
           E  PL+V  HGGP + S  S + ++ +  + G+  + VNY GS G+G +  + L  + G 
Sbjct: 490 EKPPLLVKSHGGPTAESRGSLNLNIQYWTSRGWAFVDVNYGGSTGYGREYRERLLRQWGI 549

Query: 620 QDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLA 679
            DV+D      +++  G A+  ++ + GGS GG+ T   +    D F A A+   V +L 
Sbjct: 550 VDVDDCCGCAKYLVSSGKADVKRLCISGGSAGGYTTLASLA-FRDVFKAGASLYGVADLK 609

Query: 680 LMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAK 739
           ++       +  Y++    + K++             Y  SPI  V     P I   G +
Sbjct: 610 MLKEEGHKFESRYIDNLVGDEKDF-------------YERSPINFVDKFSCPIILFQGLE 669

Query: 740 DLRVPFSNGLQFARALKEKGVDTKVIVFPDDKH 773
           D  V      +   ALK+KG+   ++ +  ++H
Sbjct: 670 DKVVTPDQSRKIYEALKKKGLPVALVEYEGEQH 688

BLAST of Carg10061 vs. TAIR 10
Match: AT5G24260.1 (prolyl oligopeptidase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 2.3e-10
Identity = 54/215 (25.12%), Postives = 94/215 (43.72%), Query Frame = 0

Query: 565 IVNLHGGP-----HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGS 624
           ++N++GGP     + +  ++      +L + G  +  ++ RG+   G      +    G 
Sbjct: 526 MINVYGGPSVQLVYDSWINTVDMRTQYLRSRGILVWKLDNRGTARRGLKFESWMKHNCGY 585

Query: 625 QDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLA 684
            D  D +     +I+QGLA P  + V G S+GG+L+  L+ + P+ F  A +  PV +  
Sbjct: 586 VDAEDQVTGAKWLIEQGLAKPDHIGVYGWSYGGYLSATLLTRYPEIFNCAVSGAPVTSW- 645

Query: 685 LMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNV--KTPTIFLLG 744
                 D  D  Y E Y           P+ E        S + HV N+  K   + + G
Sbjct: 646 ------DGYDSFYTEKY--------MGLPTEEE--RYLKSSVMHHVGNLTDKQKLMLVHG 705

Query: 745 AKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKH 773
             D  V F +  +   AL E G   ++++FPD++H
Sbjct: 706 MIDENVHFRHTARLVNALVEAGKRYELLIFPDERH 723

BLAST of Carg10061 vs. TAIR 10
Match: AT2G47390.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 5.0e-10
Identity = 46/174 (26.44%), Postives = 74/174 (42.53%), Query Frame = 0

Query: 627 AALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALM---VG 686
           AA++ ++ +G+A+ +K+AV G S+G F+T +L+  AP  F    AR+   N  L      
Sbjct: 759 AAVEEVVRRGVADRSKIAVGGHSYGAFMTANLLAHAPHLFACGIARSGAYNRTLTPFGFQ 818

Query: 687 TSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRV 746
             D   W        E  N + E            MSP    + +K P + + G +D   
Sbjct: 819 NEDRTLW--------EATNVYVE------------MSPFMSANKIKKPILLIHGEED-NN 878

Query: 747 PFSNGLQ---FARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKY 795
           P +  +Q   F  ALK  G   +++V P + H     +S          W +KY
Sbjct: 879 PGTLTMQSDRFFNALKGHGALCRLVVLPHESHGYSARESIMHVLWETDRWLQKY 911

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013512.10.0e+00100.00Acylamino-acid-releasing enzyme [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022959045.10.0e+0096.59acylamino-acid-releasing enzyme isoform X1 [Cucurbita moschata][more]
KAG6574947.10.0e+0096.47Acylamino-acid-releasing enzyme, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023548261.10.0e+0095.50acylamino-acid-releasing enzyme isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023006699.10.0e+0094.05acylamino-acid-releasing enzyme isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q84LM45.9e-26658.38Acylamino-acid-releasing enzyme OS=Arabidopsis thaliana OX=3702 GN=AARE PE=1 SV=... [more]
Q0IXP91.3e-24956.09Acylamino-acid-releasing enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q338C02.9e-23653.52Acylamino-acid-releasing enzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q8R1461.3e-9033.18Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3[more]
P802272.1e-9032.60Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H5670.0e+0096.59acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1L2W50.0e+0094.05acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1H3S30.0e+0096.37acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1L0V80.0e+0094.43acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1CDB80.0e+0079.95acylamino-acid-releasing enzyme isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G14570.14.2e-26758.38acylaminoacyl-peptidase-related [more]
AT5G36210.12.8e-1625.82alpha/beta-Hydrolases superfamily protein [more]
AT5G24260.12.3e-1025.12prolyl oligopeptidase family protein [more]
AT2G47390.15.0e-1026.44Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 542..794
e-value: 3.3E-59
score: 202.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 555..796
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 579..796
e-value: 3.1E-39
score: 134.7
NoneNo IPR availablePANTHERPTHR42776:SF24ACYLAMINO-ACID-RELEASING ENZYME-LIKEcoord: 50..446
NoneNo IPR availablePANTHERPTHR42776SERINE PEPTIDASE S9 FAMILY MEMBERcoord: 448..795
NoneNo IPR availablePANTHERPTHR42776:SF24ACYLAMINO-ACID-RELEASING ENZYME-LIKEcoord: 448..795
NoneNo IPR availablePANTHERPTHR42776SERINE PEPTIDASE S9 FAMILY MEMBERcoord: 50..446
NoneNo IPR availableSUPERFAMILY82171DPP6 N-terminal domain-likecoord: 140..410
IPR002471Peptidase S9, serine active sitePROSITEPS00708PRO_ENDOPEP_SERcoord: 624..654

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10061-RACarg10061-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity