Homology
BLAST of Carg10061 vs. NCBI nr
Match:
KAG7013512.1 (Acylamino-acid-releasing enzyme [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1635.5 bits (4234), Expect = 0.0e+00
Identity = 796/796 (100.00%), Postives = 796/796 (100.00%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
Query: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV 480
DVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV
Sbjct: 421 DVVPVVHWAKDDLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV 480
Query: 481 TIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVT 540
TIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVT
Sbjct: 481 TIPGLKYGFLIETETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVT 540
Query: 541 KGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYR 600
KGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYR
Sbjct: 541 KGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYR 600
Query: 601 GSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIG 660
GSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIG
Sbjct: 601 GSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIG 660
Query: 661 QAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMS 720
QAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMS
Sbjct: 661 QAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMS 720
Query: 721 PIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSD 780
PIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSD
Sbjct: 721 PIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSD 780
Query: 781 FESFLNICVWFKKYLK 797
FESFLNICVWFKKYLK
Sbjct: 781 FESFLNICVWFKKYLK 796
BLAST of Carg10061 vs. NCBI nr
Match:
XP_022959045.1 (acylamino-acid-releasing enzyme isoform X1 [Cucurbita moschata])
HSP 1 Score: 1615.9 bits (4183), Expect = 0.0e+00
Identity = 794/822 (96.59%), Postives = 795/822 (96.72%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
Query: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
DVVPVVHWA+DDLFPGLYSHGLLANP GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSCVS 480
Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822
BLAST of Carg10061 vs. NCBI nr
Match:
KAG6574947.1 (Acylamino-acid-releasing enzyme, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1614.0 bits (4178), Expect = 0.0e+00
Identity = 793/822 (96.47%), Postives = 794/822 (96.59%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
Query: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSE IV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSENIV 420
Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
DVVPVVHWA+DDLFPGLYSHGLLANP GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSCVS 480
Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822
BLAST of Carg10061 vs. NCBI nr
Match:
XP_023548261.1 (acylamino-acid-releasing enzyme isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 785/822 (95.50%), Postives = 792/822 (96.35%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
MALA TYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLST+LAMDGSKISAVV
Sbjct: 1 MALAGTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTALAMDGSKISAVV 60
Query: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
RKYTLSAHISEGNDDSEVNFKWTPFPVEM+GISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMIGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEHSLSLGQVVWA 300
Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSE IV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSENIV 420
Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
DVVPVVHWA+DDLFPGLYSHGLLANP GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIITTTIWRSRSAIVSIDILSGEVSCVS 480
Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
KARTLLSNILFSILKIPVKDVSNCVTKGA+EPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGATEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
CYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
LKEKGV+TKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVETKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822
BLAST of Carg10061 vs. NCBI nr
Match:
XP_023006699.1 (acylamino-acid-releasing enzyme isoform X1 [Cucurbita maxima])
HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 775/824 (94.05%), Postives = 785/824 (95.27%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAAT--APHSQSSQFLRATCLFSRKLSTSLAMDGSKISA 60
MALA TYSIGRINFLRLPLF TA T APHSQSSQFLRATCLFSRKLST+LAMDGSKISA
Sbjct: 1 MALAGTYSIGRINFLRLPLFVTAVTAPAPHSQSSQFLRATCLFSRKLSTALAMDGSKISA 60
Query: 61 VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120
VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN
Sbjct: 61 VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120
Query: 121 KKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQL 180
KKRKYTLSAHIS GNDDSEVNFKWTPFPVEM+G+STIVPSPAGSKFLAVRNPENDSPVQL
Sbjct: 121 KKRKYTLSAHISNGNDDSEVNFKWTPFPVEMIGVSTIVPSPAGSKFLAVRNPENDSPVQL 180
Query: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240
EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ
Sbjct: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240
Query: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVV 300
KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EHSLSLGQVV
Sbjct: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEHSLSLGQVV 300
Query: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDF 360
WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDE+KEDF
Sbjct: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDEAKEDF 360
Query: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSET 420
PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAH ATNSLHRIDWPRD KLSFSE
Sbjct: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHCATNSLHRIDWPRDEKLSFSEN 420
Query: 421 IVDVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSC 480
IVDVVPVVHWA+DDLFPGLYSHGLLANP GEVSC
Sbjct: 421 IVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSC 480
Query: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540
VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC
Sbjct: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540
Query: 541 STKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGP 600
STKARTLLS+ILFSILKIPVKDVSNCVTKGASEPFEAIFV+P KGNELKPLIVNLHGGP
Sbjct: 541 STKARTLLSHILFSILKIPVKDVSNCVTKGASEPFEAIFVSPFSKGNELKPLIVNLHGGP 600
Query: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660
HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI
Sbjct: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660
Query: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720
IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY
Sbjct: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720
Query: 721 VECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780
VECYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA
Sbjct: 721 VECYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780
Query: 781 RALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
RALKEKGVDTKVI+FPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 RALKEKGVDTKVIMFPDDKHPIDRPQSDFESFLNICVWFKKYLK 824
BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match:
Q84LM4 (Acylamino-acid-releasing enzyme OS=Arabidopsis thaliana OX=3702 GN=AARE PE=1 SV=1)
HSP 1 Score: 918.3 bits (2372), Expect = 5.9e-266
Identity = 453/776 (58.38%), Postives = 567/776 (73.07%), Query Frame = 0
Query: 51 MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
MD S + +E G+DP TEEEY QSKLL+EF IP+ DKAW F SDSG S F++
Sbjct: 1 MDSSGTDS-AKELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSG-SQAMFAL 60
Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
SQANLLANKK+K+ LS HIS ++ S VNF W PFP+EM G S VPSP+G K L +RNP
Sbjct: 61 SQANLLANKKKKFMLSGHISNESNQS-VNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNP 120
Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
EN+SP + EIW++ QLEKEFHIPQ VHGS+Y DGWFEGISW+S+ET++AY+AEEPS KP
Sbjct: 121 ENESPTKFEIWNSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKP 180
Query: 231 TFT-LSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISE 290
TF L Y+K S DK +WKG+GD++EEWGEAYAGKRQPALFV+N+DSGEV+ +K
Sbjct: 181 TFDHLGYYKKENSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIP 240
Query: 291 HSLSLGQVVWAP-SIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEH 350
S+S+GQVVW+P S G QYLVF GW + RK G+ YCYNRPCA+YA+K F S+
Sbjct: 241 RSISVGQVVWSPNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIK---FTSD---- 300
Query: 351 KLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWP 410
+ KD+ +FPI+NLT++ISS F PRFS DGK LVF+SA ++V++GAH AT SLHRIDWP
Sbjct: 301 EPKDDDANEFPIHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWP 360
Query: 411 RDGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP---------------------- 470
DGKL S IVDV+ VV+ KD FPGLY GLL++P
Sbjct: 361 SDGKLPESTNIVDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILS 420
Query: 471 ----CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNW 530
GEVS SP +DYSWN LALDGD+++AV S PV++P +KYG W W
Sbjct: 421 VNLLSGEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLW 480
Query: 531 VDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFV--APLPKGNE 590
++ +P+ S K LS++ F ILK+P+ DVS + +GA P EAI+V + + +
Sbjct: 481 SNIQSPI-RYSEKVMAGLSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGK 540
Query: 591 LKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQ 650
PLI LHGGPHS S SFS+++A+L+++G++ LI+NYRGSLG+GEDALQSLPGK+GSQ
Sbjct: 541 CDPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQ 600
Query: 651 DVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLAL 710
DV D L A+DH I+ G+A+P+++ VLGGSHGGFLTTHLIGQAPD+FVAAAARNPVCN+A
Sbjct: 601 DVKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMAS 660
Query: 711 MVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKD 770
MVG +DIPDWC+ E YG ++++TEAPSAE L + MSPI H+S VKTPT+FLLG KD
Sbjct: 661 MVGITDIPDWCFFEAYG--DQSHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKD 720
Query: 771 LRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
LRVP SNG Q+ RALKEKGV+ KV+VFP+D HP+DRPQ+D+ESFLNI VWF KY K
Sbjct: 721 LRVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDRPQTDYESFLNIAVWFNKYCK 763
BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match:
Q0IXP9 (Acylamino-acid-releasing enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0415600 PE=3 SV=1)
HSP 1 Score: 864.0 bits (2231), Expect = 1.3e-249
Identity = 433/772 (56.09%), Postives = 542/772 (70.21%), Query Frame = 0
Query: 57 SAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDS-GDSVVTFSISQANL 116
+A + P G+D + +EY QSKLL+EF KIP AW F S + S S+ Q +L
Sbjct: 9 AATEKGLPLGMDVSMVDEYASQSKLLQEFVKIPTIGNAWIFNSKTENTSRAIVSVGQTDL 68
Query: 117 LANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSP 176
LANKKR + L++HIS+ N + V+F+W+PFP+EM G+S ++PSP+G K L +RN E+DSP
Sbjct: 69 LANKKRSFLLNSHISK-NSSNSVDFQWSPFPIEMSGVSAVIPSPSGRKLLLIRNSEDDSP 128
Query: 177 VQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLS 236
+LE+W QLE E HI QSVHGS+Y D WFEGISWN ET +AY+AEEP KP F S
Sbjct: 129 TKLEVWGPCQLENEIHIAQSVHGSLYVDEWFEGISWNQEETLVAYVAEEPPQPKPEFNDS 188
Query: 237 GYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLG 296
GY+K S++KD +WKG+GD++E WGE Y+ KR PALFVVNI SGEV+ VK +LS+G
Sbjct: 189 GYKKAGSSEKDCKSWKGKGDWEETWGETYSKKRIPALFVVNISSGEVRAVKGIPRTLSVG 248
Query: 297 QVVWAPSIGEHQYLVFVGWSPE------PRKLGMIYCYNRPCALYAVKAPEFGSEVNEHK 356
QV+WAPS LVFV WS + PRKLG+ YC+NRPCALYAV P F E ++
Sbjct: 249 QVIWAPS--SSHSLVFVAWSSDNGYQKTPRKLGIKYCFNRPCALYAVPDP-FMEEADKPS 308
Query: 357 LKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPR 416
L E P LT +SSAF PRFSPDGK LVF+SA S++++G H+ATNS+H+IDWP
Sbjct: 309 LNVSKGETAPTTKLTSELSSAFFPRFSPDGKYLVFISAKSAIDSGTHNATNSMHKIDWPA 368
Query: 417 DGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP----------------------- 476
DGKL ++ DVVP+V +D FPGLY G+L NP
Sbjct: 369 DGKLE-GLSVADVVPIVMCPQDGCFPGLYCSGILRNPWLTDGQTMILSSIWGSKEVILSV 428
Query: 477 ---CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWV 536
EVS VSP +DYSWNVLALD DN++AV S +T+P + YG + K W W
Sbjct: 429 NVVSREVSRVSPQDSDYSWNVLALDKDNILAVSSSLITVPQIYYGSEVCQTGKPNQWEWQ 488
Query: 537 DVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKP 596
++ P + S K +L++ FSILKIP+ + SN + GA PFEAIFV+ K + +P
Sbjct: 489 EIATPFPSPSDKISAILADHKFSILKIPISNSSNKLADGAKLPFEAIFVS--WKDSATRP 548
Query: 597 LIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVN 656
IV LHGGPH+ SS+SKSLAFL + G+NLL+VNYRGSLGFGE+ALQSLPG IGSQDVN
Sbjct: 549 TIVVLHGGPHTVYPSSYSKSLAFLYSQGYNLLVVNYRGSLGFGEEALQSLPGNIGSQDVN 608
Query: 657 DVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVG 716
DVL ALD +I +GL + +KVAV+GGSHGGFLTTHLIGQAP FVAAAARNPVCNL+LMVG
Sbjct: 609 DVLTALDFVIKKGLIDASKVAVVGGSHGGFLTTHLIGQAPGTFVAAAARNPVCNLSLMVG 668
Query: 717 TSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRV 776
T+DIP+WC+VE YG+EGKN F+E PS + L + SPI H+S V TPT+FLLGA+DLRV
Sbjct: 669 TTDIPEWCFVEIYGKEGKNCFSEYPSFDDLCQFHQKSPISHISKVSTPTLFLLGAQDLRV 728
Query: 777 PFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYL 796
P SNGLQ+AR LKE GV+TK+IVFP+D H +D+PQSDFESFLNI VWFKK++
Sbjct: 729 PVSNGLQYARTLKEMGVETKIIVFPEDMHGLDKPQSDFESFLNIGVWFKKHM 773
BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match:
Q338C0 (Acylamino-acid-releasing enzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0415800 PE=3 SV=1)
HSP 1 Score: 819.7 bits (2116), Expect = 2.9e-236
Identity = 410/766 (53.52%), Postives = 534/766 (69.71%), Query Frame = 0
Query: 67 IDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGD-SVVTFSISQANLLANKKRKYTL 126
+D EEY QSKLL+EFT P+ D AW F++++ D S +SISQ NLLAN KRKY L
Sbjct: 1 MDALASEEYASQSKLLQEFTNAPSIDGAWVFQTNNEDRSTAMYSISQTNLLANNKRKYIL 60
Query: 127 SAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEIWSAGQ 186
+HI N + ++F+W+PFP++M G+S +VPSP+GSK L VRN E SP +LEI
Sbjct: 61 FSHIMR-NGTNLLDFQWSPFPIQMDGVSAVVPSPSGSKLLVVRNGEKGSPTKLEIVDQSH 120
Query: 187 LEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKGCSTDK 246
+EKE H+ QSVHG +YTD WF GISWN ET IAYIAE+ KP F +GY+K S++K
Sbjct: 121 VEKEIHVAQSVHGPLYTDEWFHGISWNQEETLIAYIAEDSPEPKPVFDDTGYRKEGSSEK 180
Query: 247 DSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWAP--SI 306
D NWKGQGD++E+WGE Y+ K +P+LFV++I+SGEV+ K SLS+GQVVWAP S
Sbjct: 181 DCNNWKGQGDWEEDWGETYSKKGRPSLFVLDINSGEVRAAKGISRSLSVGQVVWAPPSSC 240
Query: 307 GEHQYLVFVGW------SPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKED 366
G +YL+FVGW PRKLG+ YC NRPC+LY+ P S+V+ D E
Sbjct: 241 GRQKYLIFVGWLEHNGFQNTPRKLGIKYCSNRPCSLYSTLCPFEESDVDNAPASDSKLEP 300
Query: 367 FPI-YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFS 426
+ NLT +ISSAF PRFS DGK LVFLSA+ +V++GAH+AT+SLH+I+WP D K+
Sbjct: 301 ASVAINLTPSISSAFFPRFSKDGKLLVFLSANRAVDSGAHNATDSLHKINWPSDWKMDQY 360
Query: 427 ETIVDVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEV 486
I DV+P+V +D FPGLY +L+NP G+
Sbjct: 361 LEITDVIPIVMCPQDGCFPGLYCSSMLSNPWLSDRCTMILTSAWRSTEVILSIDVLSGKA 420
Query: 487 SCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDV-LNPM 546
+ +SP++++YSW+ LA+DG NV+AV S P+ P +KYG + + + W W +V NP+
Sbjct: 421 TRISPENSEYSWSALAVDGHNVLAVSSSPIDPPQIKYGHQVSLKDQTCTWVWDEVNNNPL 480
Query: 547 FNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLH 606
+ K + LLS+ FSILKIPV + S+ ++ G+ PFEAIFV+ K + KP I+ LH
Sbjct: 481 MAANNKVKALLSHHQFSILKIPVTNPSDDLSDGSKLPFEAIFVS--CKDSSHKPTILVLH 540
Query: 607 GGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAAL 666
GGPHS S SS+SK+ AFLA++GFNLLIVNYRG+ GFGE+ALQSLPGK+GSQDV D L AL
Sbjct: 541 GGPHSVSVSSYSKTSAFLASLGFNLLIVNYRGTPGFGEEALQSLPGKVGSQDVQDCLTAL 600
Query: 667 DHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPD 726
D++I+ GL + +KVAV+G SHGGFLTTHLIGQAPDRF+ AAARNPVCNL+LM+GT+DIPD
Sbjct: 601 DYVIEGGLIDASKVAVIGISHGGFLTTHLIGQAPDRFMVAAARNPVCNLSLMIGTTDIPD 660
Query: 727 WCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGL 786
WCY G EG+ + +E+PS +HL Y SPI H+S VK P + LLG DLRVP SNGL
Sbjct: 661 WCYAVACGSEGRQHASESPSPDHLRLFYQKSPIAHISKVKAPLLMLLGGADLRVPISNGL 720
Query: 787 QFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYL 796
Q+ARAL+E+G + ++++FPDD H I+ PQSDFESFLNI VWFKK+L
Sbjct: 721 QYARALRERGGEIRIMMFPDDIHEINIPQSDFESFLNIGVWFKKHL 763
BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match:
Q8R146 (Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3)
HSP 1 Score: 335.9 bits (860), Expect = 1.3e-90
Identity = 215/648 (33.18%), Postives = 326/648 (50.31%), Query Frame = 0
Query: 178 LEIWSAGQLEKEFHIPQ-SVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSG 237
LE+W + K F++ HG +Y D F +SW+ +ET++ Y+AE+ P +F +
Sbjct: 121 LEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTK 180
Query: 238 YQKGCSTDKDSTNWKG-----QGD---FKEEWGEAYAGKRQPALFVVNIDSGEVKHVKIS 297
++D++ K +GD F E+WGE K P L V++I+SG + ++
Sbjct: 181 ALDVSASDEEMARPKKPDQAIKGDQFVFYEDWGETMVSKSIPVLCVLDIESGNISVLEGV 240
Query: 298 EHSLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEH 357
++S GQ WAP +VFVGW EP +LG+ YC NR ALY V E+
Sbjct: 241 PENVSPGQAFWAPG---DTGVVFVGWWHEPFRLGIRYCTNRRSALYYVDLSGGKCEL--- 300
Query: 358 KLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDW- 417
L DES + SPR SPD +V+L S H + L DW
Sbjct: 301 -LSDES-------------LAVCSPRLSPDQCRVVYLQYPS---LAPHHQCSQLFLYDWY 360
Query: 418 -----------PRDGKLSFSETIVDVVPVVHWAKDD---LFPGLY--SHGLLA--NPCGE 477
PR SFS ++P+ W+ D +F + L A G
Sbjct: 361 TKVTSLVVDIVPRQLGESFSGIYCSLLPLGCWSADSQRVVFDSVQRSRQDLFAVDTQTGS 420
Query: 478 VSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIET-ETKDVVWNWVDVLNP 537
V+ ++ + SW +L +D D ++A S P P LK GFL + + V W ++ P
Sbjct: 421 VTSLTAGGSAGSWKLLTIDRDLMVAQFSTPNLPPSLKVGFLPPAGKEQSVSWVSLEEAEP 480
Query: 538 MFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELK-PLIVN 597
+ + R L P D N + A FEAI + P ++ + P++V
Sbjct: 481 IPDIHWGIRVL----------HPPPDQEN--VQYADLDFEAILLQPSNSPDKSQVPMVVM 540
Query: 598 LHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLA 657
HGGPHS+ +++ A L +GF +L+VNYRGS GFG+D++ SLPG +G QDV DV
Sbjct: 541 PHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQF 600
Query: 658 ALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDI 717
A+ ++ + + +VA++GGSHGGFL+ HLIGQ P+ + A ARNPV N+ M+GT+DI
Sbjct: 601 AVQQVLQEEHFDARRVALMGGSHGGFLSCHLIGQYPETYSACIARNPVINIVSMMGTTDI 660
Query: 718 PDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSN 777
PDWC VE +Y P L + SPI+++ VKTP + +LG +D RVPF
Sbjct: 661 PDWCMVETGFPYSNDYL---PDLNVLEEMLDKSPIKYIPQVKTPVLLMLGQEDRRVPFKQ 720
Query: 778 GLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYL 796
GL++ ALK + V +++++P H + + + +SF+N +W +L
Sbjct: 721 GLEYYHALKARNVPVRLLLYPKSTHALSEVEVESDSFMNTVLWLHTHL 730
BLAST of Carg10061 vs. ExPASy Swiss-Prot
Match:
P80227 (Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2)
HSP 1 Score: 335.1 bits (858), Expect = 2.1e-90
Identity = 221/678 (32.60%), Postives = 332/678 (48.97%), Query Frame = 0
Query: 158 SPAGSKFLAVRNPENDSPVQ--LEIWSAGQLEKEFHIPQ-SVHGSIYTDGWFEGISWNSN 217
SP+G+ +R + + LE+W + K F++ HG +Y D F +SW+ +
Sbjct: 97 SPSGTMKAVLRKAGSTGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHS 156
Query: 218 ETYIAYIAEEPSPSKPTF------TLSGYQKGCSTDK--DSTNWKGQGDFKEEWGEAYAG 277
ET++ Y+AE+ P +F +SG + K D Q F E+WGE
Sbjct: 157 ETHLLYVAEKKRPKAESFFQTKALDISGSDDEMARPKKPDQAIKGDQFLFYEDWGENMVS 216
Query: 278 KRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYC 337
K P L V++I+SG + ++ S+S GQ WAP +VF GW EP +LG+ +C
Sbjct: 217 KGSPVLCVLDIESGNISVLEGVPESVSPGQAFWAPG---DTGVVFAGWWHEPFRLGIRFC 276
Query: 338 YNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLS 397
NR ALY V E+ L D+S + SPR SPD +V+L
Sbjct: 277 TNRRSALYYVDLTGGNCEL----LSDDS-------------LAVTSPRLSPDQCRIVYLQ 336
Query: 398 AHSSVNTGAHSATNSLHRIDW------------PRDGKLSFSETIVDVVPVVHWAKD--- 457
S V H L DW PR +FS ++P+ W+ D
Sbjct: 337 FPSLV---PHQQCGQLCLYDWYTRVTVVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQR 396
Query: 458 -----------DLFPGLYSHGLLANPCGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPV 517
DLF + G V+ ++ + SW +L +D D ++A S P
Sbjct: 397 VVFDTAQRSRQDLF-------AVDTQMGTVTPLTAGGSGGSWKLLTIDRDLMVAQFSTPN 456
Query: 518 TIPGLKYGFLIET-ETKDVVWNWVDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCV 577
P LK GFL ++VVW ++ P+ + S R L P + +
Sbjct: 457 LPPCLKVGFLPPAGMEQEVVWVSLEEAEPIPDISWSIRVLQ----------PPPEQEHAQ 516
Query: 578 TKGASEPFEAIFVAPL-PKGNELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVN 637
G FEAI + P P P++V HGGPHS+ +S+ A L +GF L+VN
Sbjct: 517 YVGLD--FEAILIQPSNPPDKTQVPMVVMPHGGPHSSFVTSWMLLPAMLCKMGFAALLVN 576
Query: 638 YRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHL 697
YRGS GFG+D++ SLPG +GSQDV DV A++ ++ + + +VA+LGGSHGGFL+ HL
Sbjct: 577 YRGSTGFGQDSILSLPGNVGSQDVKDVQFAVEQVLQEEHFDAGRVALLGGSHGGFLSCHL 636
Query: 698 IGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVECYGREGKNYFTEA-PSAEHLIHLY 757
IGQ P+ + A RNPV N+A M+G++DIPDWC VE G Y ++ P +
Sbjct: 637 IGQYPETYGACVVRNPVINIASMMGSTDIPDWCVVEA----GYLYSSDCLPDPNVWSEML 696
Query: 758 HMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRP 796
+ SPI++ VKTP + +LG +D RVPF G+++ RALK + V +++++P H +
Sbjct: 697 NKSPIKYTPQVKTPVLLMLGQEDRRVPFKQGMEYYRALKARNVPVRLLLYPKSTHSLSEV 728
BLAST of Carg10061 vs. ExPASy TrEMBL
Match:
A0A6J1H567 (acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460152 PE=4 SV=1)
HSP 1 Score: 1615.9 bits (4183), Expect = 0.0e+00
Identity = 794/822 (96.59%), Postives = 795/822 (96.72%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV
Sbjct: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
Query: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK
Sbjct: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI
Sbjct: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG
Sbjct: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA
Sbjct: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI
Sbjct: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV
Sbjct: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
DVVPVVHWA+DDLFPGLYSHGLLANP GEVSCVS
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSCVS 480
Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST
Sbjct: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS
Sbjct: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGPHS 600
Query: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID
Sbjct: 601 TSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHIID 660
Query: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE
Sbjct: 661 QGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYVE 720
Query: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA
Sbjct: 721 CYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFARA 780
Query: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 LKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 822
BLAST of Carg10061 vs. ExPASy TrEMBL
Match:
A0A6J1L2W5 (acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499357 PE=4 SV=1)
HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 775/824 (94.05%), Postives = 785/824 (95.27%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAAT--APHSQSSQFLRATCLFSRKLSTSLAMDGSKISA 60
MALA TYSIGRINFLRLPLF TA T APHSQSSQFLRATCLFSRKLST+LAMDGSKISA
Sbjct: 1 MALAGTYSIGRINFLRLPLFVTAVTAPAPHSQSSQFLRATCLFSRKLSTALAMDGSKISA 60
Query: 61 VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120
VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN
Sbjct: 61 VVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLAN 120
Query: 121 KKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQL 180
KKRKYTLSAHIS GNDDSEVNFKWTPFPVEM+G+STIVPSPAGSKFLAVRNPENDSPVQL
Sbjct: 121 KKRKYTLSAHISNGNDDSEVNFKWTPFPVEMIGVSTIVPSPAGSKFLAVRNPENDSPVQL 180
Query: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240
EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ
Sbjct: 181 EIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQ 240
Query: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVV 300
KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EHSLSLGQVV
Sbjct: 241 KGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEHSLSLGQVV 300
Query: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDF 360
WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDE+KEDF
Sbjct: 301 WAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDEAKEDF 360
Query: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSET 420
PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAH ATNSLHRIDWPRD KLSFSE
Sbjct: 361 PIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHCATNSLHRIDWPRDEKLSFSEN 420
Query: 421 IVDVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSC 480
IVDVVPVVHWA+DDLFPGLYSHGLLANP GEVSC
Sbjct: 421 IVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSIDILSGEVSC 480
Query: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540
VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC
Sbjct: 481 VSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNC 540
Query: 541 STKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPLIVNLHGGP 600
STKARTLLS+ILFSILKIPVKDVSNCVTKGASEPFEAIFV+P KGNELKPLIVNLHGGP
Sbjct: 541 STKARTLLSHILFSILKIPVKDVSNCVTKGASEPFEAIFVSPFSKGNELKPLIVNLHGGP 600
Query: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660
HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI
Sbjct: 601 HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHI 660
Query: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720
IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY
Sbjct: 661 IDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCY 720
Query: 721 VECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780
VECYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA
Sbjct: 721 VECYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFA 780
Query: 781 RALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
RALKEKGVDTKVI+FPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 781 RALKEKGVDTKVIMFPDDKHPIDRPQSDFESFLNICVWFKKYLK 824
BLAST of Carg10061 vs. ExPASy TrEMBL
Match:
A0A6J1H3S3 (acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460152 PE=4 SV=1)
HSP 1 Score: 1525.4 bits (3948), Expect = 0.0e+00
Identity = 744/772 (96.37%), Postives = 745/772 (96.50%), Query Frame = 0
Query: 51 MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI
Sbjct: 1 MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 60
Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP
Sbjct: 61 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 120
Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP
Sbjct: 121 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 180
Query: 231 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH 290
TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH
Sbjct: 181 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH 240
Query: 291 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 350
SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL
Sbjct: 241 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 300
Query: 351 KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD 410
KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD
Sbjct: 301 KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD 360
Query: 411 GKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP------------------------ 470
GKLSFSETIVDVVPVVHWA+DDLFPGLYSHGLLANP
Sbjct: 361 GKLSFSETIVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSID 420
Query: 471 --CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 530
GEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD
Sbjct: 421 ILSGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 480
Query: 531 VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL 590
VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL
Sbjct: 481 VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL 540
Query: 591 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 650
IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND
Sbjct: 541 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 600
Query: 651 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 710
VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT
Sbjct: 601 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 660
Query: 711 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP 770
SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP
Sbjct: 661 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP 720
Query: 771 FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 721 FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 772
BLAST of Carg10061 vs. ExPASy TrEMBL
Match:
A0A6J1L0V8 (acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499357 PE=4 SV=1)
HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 729/772 (94.43%), Postives = 738/772 (95.60%), Query Frame = 0
Query: 51 MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI
Sbjct: 1 MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 60
Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
SQANLLANKKRKYTLSAHIS GNDDSEVNFKWTPFPVEM+G+STIVPSPAGSKFLAVRNP
Sbjct: 61 SQANLLANKKRKYTLSAHISNGNDDSEVNFKWTPFPVEMIGVSTIVPSPAGSKFLAVRNP 120
Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP
Sbjct: 121 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 180
Query: 231 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEH 290
TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFV+NIDSGEVKHVKI EH
Sbjct: 181 TFTLSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVINIDSGEVKHVKIIEH 240
Query: 291 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 350
SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL
Sbjct: 241 SLSLGQVVWAPSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKL 300
Query: 351 KDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRD 410
KDE+KEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAH ATNSLHRIDWPRD
Sbjct: 301 KDEAKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHCATNSLHRIDWPRD 360
Query: 411 GKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP------------------------ 470
KLSFSE IVDVVPVVHWA+DDLFPGLYSHGLLANP
Sbjct: 361 EKLSFSENIVDVVPVVHWAEDDLFPGLYSHGLLANPWFSDGHTIIITTIWRSRSAIVSID 420
Query: 471 --CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 530
GEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD
Sbjct: 421 ILSGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVD 480
Query: 531 VLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLPKGNELKPL 590
VLNPMFNCSTKARTLLS+ILFSILKIPVKDVSNCVTKGASEPFEAIFV+P KGNELKPL
Sbjct: 481 VLNPMFNCSTKARTLLSHILFSILKIPVKDVSNCVTKGASEPFEAIFVSPFSKGNELKPL 540
Query: 591 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 650
IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND
Sbjct: 541 IVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVND 600
Query: 651 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 710
VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT
Sbjct: 601 VLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGT 660
Query: 711 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVP 770
SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLY+MSPIRHVSNVKTPTIFLLGAKDLRVP
Sbjct: 661 SDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYNMSPIRHVSNVKTPTIFLLGAKDLRVP 720
Query: 771 FSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
FSNGLQFARALKEKGVDTKVI+FPDDKHPIDRPQSDFESFLNICVWFKKYLK
Sbjct: 721 FSNGLQFARALKEKGVDTKVIMFPDDKHPIDRPQSDFESFLNICVWFKKYLK 772
BLAST of Carg10061 vs. ExPASy TrEMBL
Match:
A0A6J1CDB8 (acylamino-acid-releasing enzyme isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009690 PE=4 SV=1)
HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 658/823 (79.95%), Postives = 720/823 (87.48%), Query Frame = 0
Query: 1 MALASTYSIGRINFLRLPLFATAATAPHSQSSQFLRATCLFSRKLSTSLAMDGSKISAVV 60
MAL IGR++ LRLPLFA AATA S+SS F RAT LF+RKLSTS AMDGSKISAVV
Sbjct: 1 MALTGIAPIGRVSLLRLPLFAIAATATPSRSSPFFRATSLFTRKLSTSFAMDGSKISAVV 60
Query: 61 EEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSISQANLLANKK 120
+E P+G+DP TE EY EQSKLL+EFT IPN DKAW FKSDSGDSV TFSISQA+LL+NK+
Sbjct: 61 DELPAGVDPETEGEYAEQSKLLQEFTTIPNIDKAWMFKSDSGDSVATFSISQASLLSNKR 120
Query: 121 RKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNPENDSPVQLEI 180
RKYTLSAHIS+GN DS VNFKW PFPVEM+G+STIVPSP+GSK L VRN ENDSPV+LEI
Sbjct: 121 RKYTLSAHISKGNGDSSVNFKWAPFPVEMIGVSTIVPSPSGSKLLTVRNSENDSPVKLEI 180
Query: 181 WSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTLSGYQKG 240
WSAGQ+EKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFT+SGYQKG
Sbjct: 181 WSAGQIEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKPTFTVSGYQKG 240
Query: 241 CSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISEHSLSLGQVVWA 300
CST+KDSTNWK QGDF+EEWGEAYAGKRQPALFV+NIDSGEV+HVK+ E LS+GQVVWA
Sbjct: 241 CSTNKDSTNWKAQGDFEEEWGEAYAGKRQPALFVINIDSGEVRHVKLIEKYLSVGQVVWA 300
Query: 301 PSIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEHKLKDESKEDFPI 360
PSIG HQYLVF GWS EPRKLGM YCYNRPCALYAV+AP++GSEV+EHKLKD SKEDFP+
Sbjct: 301 PSIGVHQYLVFAGWSSEPRKLGMKYCYNRPCALYAVRAPDYGSEVDEHKLKDGSKEDFPL 360
Query: 361 YNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWPRDGKLSFSETIV 420
YNLTQNISSAF PRFSPDGK L+FLSA+SSVNTGAHSATNSLHRI+WP D KL F+E IV
Sbjct: 361 YNLTQNISSAFLPRFSPDGKFLIFLSANSSVNTGAHSATNSLHRINWPTDEKLCFNENIV 420
Query: 421 DVVPVVHWAKDDLFPGLYSHGLLANP--------------------------CGEVSCVS 480
DVVPVVHWA+DDLFPGLYSHG L NP GEVS +S
Sbjct: 421 DVVPVVHWAEDDLFPGLYSHGFLINPWLSDGRTVIISSIWHSSEAILSIDILSGEVSRIS 480
Query: 481 PDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNWVDVLNPMFNCST 540
P ++++SWNVLALDGDNV+AV S PV IP LKYG LIE ETK+ VW+W+DV +P+F CS
Sbjct: 481 PSNSNFSWNVLALDGDNVVAVSSSPVDIPQLKYGRLIEKETKNAVWSWLDVSSPVFKCSE 540
Query: 541 KARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFVAPLP-KGNELKPLIVNLHGGPH 600
K +TLLS + FSI+KIPVKDVSNC+TKGAS+PFEAIFV+ KGNEL P+IVNLHGGPH
Sbjct: 541 KVKTLLSALQFSIMKIPVKDVSNCLTKGASKPFEAIFVSSKSVKGNELNPVIVNLHGGPH 600
Query: 601 STSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQDVNDVLAALDHII 660
STS SSFSKS AFL+++GFNLL+VNYRGSLGFGEDALQSLPGK+GSQDVNDVLAALDHII
Sbjct: 601 STSISSFSKSSAFLSSLGFNLLVVNYRGSLGFGEDALQSLPGKVGSQDVNDVLAALDHII 660
Query: 661 DQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYV 720
D+GLANP+KVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYV
Sbjct: 661 DKGLANPSKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALMVGTSDIPDWCYV 720
Query: 721 ECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRVPFSNGLQFAR 780
ECYGREGKNYFTEAPSAEH I LY+ SPI HVS VK PTIFLLGAKDLRVPFSNGLQFAR
Sbjct: 721 ECYGREGKNYFTEAPSAEHQIELYNKSPILHVSKVKAPTIFLLGAKDLRVPFSNGLQFAR 780
Query: 781 ALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
ALKEKGV+ KVI+FPDD HPIDRPQSDFESFLNI VWFKKY K
Sbjct: 781 ALKEKGVEVKVIMFPDDIHPIDRPQSDFESFLNIGVWFKKYCK 823
BLAST of Carg10061 vs. TAIR 10
Match:
AT4G14570.1 (acylaminoacyl-peptidase-related )
HSP 1 Score: 918.3 bits (2372), Expect = 4.2e-267
Identity = 453/776 (58.38%), Postives = 567/776 (73.07%), Query Frame = 0
Query: 51 MDGSKISAVVEEFPSGIDPATEEEYVEQSKLLEEFTKIPNFDKAWTFKSDSGDSVVTFSI 110
MD S + +E G+DP TEEEY QSKLL+EF IP+ DKAW F SDSG S F++
Sbjct: 1 MDSSGTDS-AKELHVGLDPTTEEEYATQSKLLQEFINIPSIDKAWIFNSDSG-SQAMFAL 60
Query: 111 SQANLLANKKRKYTLSAHISEGNDDSEVNFKWTPFPVEMVGISTIVPSPAGSKFLAVRNP 170
SQANLLANKK+K+ LS HIS ++ S VNF W PFP+EM G S VPSP+G K L +RNP
Sbjct: 61 SQANLLANKKKKFMLSGHISNESNQS-VNFHWAPFPIEMTGASAFVPSPSGLKLLVIRNP 120
Query: 171 ENDSPVQLEIWSAGQLEKEFHIPQSVHGSIYTDGWFEGISWNSNETYIAYIAEEPSPSKP 230
EN+SP + EIW++ QLEKEFHIPQ VHGS+Y DGWFEGISW+S+ET++AY+AEEPS KP
Sbjct: 121 ENESPTKFEIWNSSQLEKEFHIPQKVHGSVYVDGWFEGISWDSDETHVAYVAEEPSRPKP 180
Query: 231 TFT-LSGYQKGCSTDKDSTNWKGQGDFKEEWGEAYAGKRQPALFVVNIDSGEVKHVKISE 290
TF L Y+K S DK +WKG+GD++EEWGEAYAGKRQPALFV+N+DSGEV+ +K
Sbjct: 181 TFDHLGYYKKENSLDKGIGSWKGEGDWEEEWGEAYAGKRQPALFVINVDSGEVEPIKGIP 240
Query: 291 HSLSLGQVVWAP-SIGEHQYLVFVGWSPEPRKLGMIYCYNRPCALYAVKAPEFGSEVNEH 350
S+S+GQVVW+P S G QYLVF GW + RK G+ YCYNRPCA+YA+K F S+
Sbjct: 241 RSISVGQVVWSPNSNGSAQYLVFAGWLGDKRKFGIKYCYNRPCAIYAIK---FTSD---- 300
Query: 351 KLKDESKEDFPIYNLTQNISSAFSPRFSPDGKSLVFLSAHSSVNTGAHSATNSLHRIDWP 410
+ KD+ +FPI+NLT++ISS F PRFS DGK LVF+SA ++V++GAH AT SLHRIDWP
Sbjct: 301 EPKDDDANEFPIHNLTKSISSGFCPRFSKDGKFLVFVSAKTAVDSGAHWATESLHRIDWP 360
Query: 411 RDGKLSFSETIVDVVPVVHWAKDDLFPGLYSHGLLANP---------------------- 470
DGKL S IVDV+ VV+ KD FPGLY GLL++P
Sbjct: 361 SDGKLPESTNIVDVIQVVNCPKDGCFPGLYVTGLLSDPWLSDGHSLMLSTYWRSCRVILS 420
Query: 471 ----CGEVSCVSPDHTDYSWNVLALDGDNVIAVRSGPVTIPGLKYGFLIETETKDVVWNW 530
GEVS SP +DYSWN LALDGD+++AV S PV++P +KYG W W
Sbjct: 421 VNLLSGEVSRASPSDSDYSWNALALDGDSIVAVSSSPVSVPEIKYGKKGLDSAGKPSWLW 480
Query: 531 VDVLNPMFNCSTKARTLLSNILFSILKIPVKDVSNCVTKGASEPFEAIFV--APLPKGNE 590
++ +P+ S K LS++ F ILK+P+ DVS + +GA P EAI+V + + +
Sbjct: 481 SNIQSPI-RYSEKVMAGLSSLQFKILKVPISDVSEGLAEGAKNPIEAIYVSSSKSKENGK 540
Query: 591 LKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGSQ 650
PLI LHGGPHS S SFS+++A+L+++G++ LI+NYRGSLG+GEDALQSLPGK+GSQ
Sbjct: 541 CDPLIAVLHGGPHSVSPCSFSRTMAYLSSIGYSQLIINYRGSLGYGEDALQSLPGKVGSQ 600
Query: 651 DVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLAL 710
DV D L A+DH I+ G+A+P+++ VLGGSHGGFLTTHLIGQAPD+FVAAAARNPVCN+A
Sbjct: 601 DVKDCLLAVDHAIEMGIADPSRITVLGGSHGGFLTTHLIGQAPDKFVAAAARNPVCNMAS 660
Query: 711 MVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKD 770
MVG +DIPDWC+ E YG ++++TEAPSAE L + MSPI H+S VKTPT+FLLG KD
Sbjct: 661 MVGITDIPDWCFFEAYG--DQSHYTEAPSAEDLSRFHQMSPISHISKVKTPTLFLLGTKD 720
Query: 771 LRVPFSNGLQFARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKYLK 797
LRVP SNG Q+ RALKEKGV+ KV+VFP+D HP+DRPQ+D+ESFLNI VWF KY K
Sbjct: 721 LRVPISNGFQYVRALKEKGVEVKVLVFPNDNHPLDRPQTDYESFLNIAVWFNKYCK 763
BLAST of Carg10061 vs. TAIR 10
Match:
AT5G36210.1 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 85.1 bits (209), Expect = 2.8e-16
Identity = 55/213 (25.82%), Postives = 97/213 (45.54%), Query Frame = 0
Query: 560 ELKPLIVNLHGGPHSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGS 619
E PL+V HGGP + S S + ++ + + G+ + VNY GS G+G + + L + G
Sbjct: 490 EKPPLLVKSHGGPTAESRGSLNLNIQYWTSRGWAFVDVNYGGSTGYGREYRERLLRQWGI 549
Query: 620 QDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLA 679
DV+D +++ G A+ ++ + GGS GG+ T + D F A A+ V +L
Sbjct: 550 VDVDDCCGCAKYLVSSGKADVKRLCISGGSAGGYTTLASLA-FRDVFKAGASLYGVADLK 609
Query: 680 LMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAK 739
++ + Y++ + K++ Y SPI V P I G +
Sbjct: 610 MLKEEGHKFESRYIDNLVGDEKDF-------------YERSPINFVDKFSCPIILFQGLE 669
Query: 740 DLRVPFSNGLQFARALKEKGVDTKVIVFPDDKH 773
D V + ALK+KG+ ++ + ++H
Sbjct: 670 DKVVTPDQSRKIYEALKKKGLPVALVEYEGEQH 688
BLAST of Carg10061 vs. TAIR 10
Match:
AT5G24260.1 (prolyl oligopeptidase family protein )
HSP 1 Score: 65.5 bits (158), Expect = 2.3e-10
Identity = 54/215 (25.12%), Postives = 94/215 (43.72%), Query Frame = 0
Query: 565 IVNLHGGP-----HSTSTSSFSKSLAFLAAVGFNLLIVNYRGSLGFGEDALQSLPGKIGS 624
++N++GGP + + ++ +L + G + ++ RG+ G + G
Sbjct: 526 MINVYGGPSVQLVYDSWINTVDMRTQYLRSRGILVWKLDNRGTARRGLKFESWMKHNCGY 585
Query: 625 QDVNDVLAALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLA 684
D D + +I+QGLA P + V G S+GG+L+ L+ + P+ F A + PV +
Sbjct: 586 VDAEDQVTGAKWLIEQGLAKPDHIGVYGWSYGGYLSATLLTRYPEIFNCAVSGAPVTSW- 645
Query: 685 LMVGTSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNV--KTPTIFLLG 744
D D Y E Y P+ E S + HV N+ K + + G
Sbjct: 646 ------DGYDSFYTEKY--------MGLPTEEE--RYLKSSVMHHVGNLTDKQKLMLVHG 705
Query: 745 AKDLRVPFSNGLQFARALKEKGVDTKVIVFPDDKH 773
D V F + + AL E G ++++FPD++H
Sbjct: 706 MIDENVHFRHTARLVNALVEAGKRYELLIFPDERH 723
BLAST of Carg10061 vs. TAIR 10
Match:
AT2G47390.1 (Prolyl oligopeptidase family protein )
HSP 1 Score: 64.3 bits (155), Expect = 5.0e-10
Identity = 46/174 (26.44%), Postives = 74/174 (42.53%), Query Frame = 0
Query: 627 AALDHIIDQGLANPAKVAVLGGSHGGFLTTHLIGQAPDRFVAAAARNPVCNLALM---VG 686
AA++ ++ +G+A+ +K+AV G S+G F+T +L+ AP F AR+ N L
Sbjct: 759 AAVEEVVRRGVADRSKIAVGGHSYGAFMTANLLAHAPHLFACGIARSGAYNRTLTPFGFQ 818
Query: 687 TSDIPDWCYVECYGREGKNYFTEAPSAEHLIHLYHMSPIRHVSNVKTPTIFLLGAKDLRV 746
D W E N + E MSP + +K P + + G +D
Sbjct: 819 NEDRTLW--------EATNVYVE------------MSPFMSANKIKKPILLIHGEED-NN 878
Query: 747 PFSNGLQ---FARALKEKGVDTKVIVFPDDKHPIDRPQSDFESFLNICVWFKKY 795
P + +Q F ALK G +++V P + H +S W +KY
Sbjct: 879 PGTLTMQSDRFFNALKGHGALCRLVVLPHESHGYSARESIMHVLWETDRWLQKY 911
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7013512.1 | 0.0e+00 | 100.00 | Acylamino-acid-releasing enzyme [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022959045.1 | 0.0e+00 | 96.59 | acylamino-acid-releasing enzyme isoform X1 [Cucurbita moschata] | [more] |
KAG6574947.1 | 0.0e+00 | 96.47 | Acylamino-acid-releasing enzyme, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_023548261.1 | 0.0e+00 | 95.50 | acylamino-acid-releasing enzyme isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_023006699.1 | 0.0e+00 | 94.05 | acylamino-acid-releasing enzyme isoform X1 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q84LM4 | 5.9e-266 | 58.38 | Acylamino-acid-releasing enzyme OS=Arabidopsis thaliana OX=3702 GN=AARE PE=1 SV=... | [more] |
Q0IXP9 | 1.3e-249 | 56.09 | Acylamino-acid-releasing enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... | [more] |
Q338C0 | 2.9e-236 | 53.52 | Acylamino-acid-releasing enzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... | [more] |
Q8R146 | 1.3e-90 | 33.18 | Acylamino-acid-releasing enzyme OS=Mus musculus OX=10090 GN=Apeh PE=1 SV=3 | [more] |
P80227 | 2.1e-90 | 32.60 | Acylamino-acid-releasing enzyme OS=Bos taurus OX=9913 GN=APEH PE=1 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1H567 | 0.0e+00 | 96.59 | acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
A0A6J1L2W5 | 0.0e+00 | 94.05 | acylamino-acid-releasing enzyme isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
A0A6J1H3S3 | 0.0e+00 | 96.37 | acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
A0A6J1L0V8 | 0.0e+00 | 94.43 | acylamino-acid-releasing enzyme isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
A0A6J1CDB8 | 0.0e+00 | 79.95 | acylamino-acid-releasing enzyme isoform X2 OS=Momordica charantia OX=3673 GN=LOC... | [more] |