Carg09853 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09853
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMFS domain-containing protein
LocationCarg_Chr10: 2888676 .. 2890800 (-)
RNA-Seq ExpressionCarg09853
SyntenyCarg09853
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAACAATCTCTAAATAATGGAATCTTTCTACATAAGGGGATAAAAGGAGTGGGGAGTAAGTTCCAGCTCATACGAAACTAGGAGATTCAAAAGATGACGTGTAATTCATATTCCTCCTAATATCATGCCATTCATGCAGATTTGGCAATCACTAAACCCCTCGAAGAATTAAATTTCCACTCACCTAACCGAACGCACCACAATTACAGCTTCTCCTCTGCTCCATTTTCGCCCATTCAAATAACGGCCCACTGATTCTCGTAATCCCGTTCAAGAAGAAAAAGCAACAATCCCTCCGACATGGAAGCCGCTGCTGCAACTCCGATGGAACCCATGGATGTGGAAGAAGCCTCCAAACTGCAACCCAAGGGATTTGGGATCAACAAGTACACCTTAATTTGCTCCATTTTGGCCTCCACCAACTCCATTTTGTTGGGCTACGGTCAGTTCCTTGTCTCTCTTTTGCTTTTTTGCGTTTTGGGTTTCTGAGTTTTGGCTCAATTCGTGAAGATATTGGAGTGATGAGTGGGGCTGTGCTCTACATCGAAGAGAATCTCAAAATCTCATCCACCCAAGTGGAAATCCTTGTGGGATCGCTAAACGTACTCTCTCTAGTCGGATCTCTCGCTTCCGGGCGGACCTCTGATTCAATCGGCCGGCGATACACGACATTCCTCGCATCCACCACGTTTCTAATCGGCGCGTTGTTAATGGGTCTCGCCCCATCTTTTCCTCTTCTCCTAGTCGGTCGTATGATCGCTGGCGTCGGCGTCGGTTACGCCCTCATGATCGCCCCTGTCTACACGGCGGAGCTCTCTCCGGCTACATCCCGCGGCCTTCTCAGTTCATTCCCGGAAATCTTCATCACGTTCGGTATTTTACTGGGCTACATCATCAACTACGCTCTGTCCGGCCTACCACCGCACATCAACTGGAGAATCATGCTCGGAATCGCCGGAATACCAGCGCTGGGCGTCGGATTGGGCGTTCTAACAATGCCCGAATCCCCGCGTTGGTTAGTCATGAAGGGCAAATCGGAAGAAGCCAAAAAGGTCTTAATCAAAATCTCCACCAACGAAATCGAAGCAGAGGAGAGACTGAAGTCAATAACCGAAGCCGTAGCCGCAGCCGCGCCCGGCGGCAAATGGAACGGACAAGGGGTTTGGAAGGAACTACTGATAAAACCCACAAAGCCAATCCGCCGAATACTAATAGCCGCCATCGGAATCAACTTCTTCATGCAAGCATCAGGAAACGACGCCGTAATGTACTACTCACCGGAAGTATTCCGCGCAGCCGGAATCCAGCGAAAACGGCATCTGTTCAGCGTGAACGTGGTAATGGGCATTACAAAGACCTGCTTCGTTCTATTCTCAGCACTGTACCTAGACCGATTAGGAAGGCGGCCGCTTTTACTAGTGGGGTCAGCCGGGATGACAGTGGCGCTAGGCTTACTAGCCCTGGGCTCAAAAGCAATGGAAATGGGAAAAGGCAGGCCAACATGGGCCGTAGTATTGAGCATAGTAGCATTATGTGGGGACGTGGCATTATTTTCAATTGGGCTTGGGCCAATAGCGTGGGTGTACTCATCGGAGATATTCCCGAACAGGATGAGGGCGCAGGGGTCGAGCCTGGCGGTATCAGTGAACAGATTGGTGAGCGGAGTGGTATCGATGACATTTCTGAGCATATCAAAAGAGATCACGTTTGGAGGCATGTTCATGGTGCTATCGGGAATAATGGCACTGGGTACCCTCTTCTTTTACTTCTTTTTGCCAGAGACTAAAGGAAAAAGCTTGGAGGAGATGGAGGTCCTGTTCCAGGACAAGGACACAAACAGTACTACGCCACTGCAGATTGAAGAGACATTGTAGTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTACCTACTACCTTTTCCCTTATTTCTTCATCACTTTATGTTCATGTTTTGCGTAGCATTGTGGATCATCATGGATGAGCTGATGAACTCTCATAGGATCAAAGTTTATTATTTATTATTAAGATGAACATAGCTTAGTTGGGATTAAACTTTTGGGTTGAAAATTTGAAATACTTTGTGGGGGGATGTATGATTGGTCAAGTCAAGGGTGGGAATGAAA

mRNA sequence

GGAAAACAATCTCTAAATAATGGAATCTTTCTACATAAGGGGATAAAAGGAGTGGGGAGTAAGTTCCAGCTCATACGAAACTAGGAGATTCAAAAGATGACGTGTAATTCATATTCCTCCTAATATCATGCCATTCATGCAGATTTGGCAATCACTAAACCCCTCGAAGAATTAAATTTCCACTCACCTAACCGAACGCACCACAATTACAGCTTCTCCTCTGCTCCATTTTCGCCCATTCAAATAACGGCCCACTGATTCTCGTAATCCCGTTCAAGAAGAAAAAGCAACAATCCCTCCGACATGGAAGCCGCTGCTGCAACTCCGATGGAACCCATGGATGTGGAAGAAGCCTCCAAACTGCAACCCAAGGGATTTGGGATCAACAAGTACACCTTAATTTGCTCCATTTTGGCCTCCACCAACTCCATTTTGTTGGGCTACGATATTGGAGTGATGAGTGGGGCTGTGCTCTACATCGAAGAGAATCTCAAAATCTCATCCACCCAAGTGGAAATCCTTGTGGGATCGCTAAACGTACTCTCTCTAGTCGGATCTCTCGCTTCCGGGCGGACCTCTGATTCAATCGGCCGGCGATACACGACATTCCTCGCATCCACCACGTTTCTAATCGGCGCGTTGTTAATGGGTCTCGCCCCATCTTTTCCTCTTCTCCTAGTCGGTCGTATGATCGCTGGCGTCGGCGTCGGTTACGCCCTCATGATCGCCCCTGTCTACACGGCGGAGCTCTCTCCGGCTACATCCCGCGGCCTTCTCAGTTCATTCCCGGAAATCTTCATCACGTTCGGTATTTTACTGGGCTACATCATCAACTACGCTCTGTCCGGCCTACCACCGCACATCAACTGGAGAATCATGCTCGGAATCGCCGGAATACCAGCGCTGGGCGTCGGATTGGGCGTTCTAACAATGCCCGAATCCCCGCGTTGGTTAGTCATGAAGGGCAAATCGGAAGAAGCCAAAAAGGTCTTAATCAAAATCTCCACCAACGAAATCGAAGCAGAGGAGAGACTGAAGTCAATAACCGAAGCCGTAGCCGCAGCCGCGCCCGGCGGCAAATGGAACGGACAAGGGGTTTGGAAGGAACTACTGATAAAACCCACAAAGCCAATCCGCCGAATACTAATAGCCGCCATCGGAATCAACTTCTTCATGCAAGCATCAGGAAACGACGCCGTAATGTACTACTCACCGGAAGTATTCCGCGCAGCCGGAATCCAGCGAAAACGGCATCTGTTCAGCGTGAACGTGGTAATGGGCATTACAAAGACCTGCTTCGTTCTATTCTCAGCACTGTACCTAGACCGATTAGGAAGGCGGCCGCTTTTACTAGTGGGGTCAGCCGGGATGACAGTGGCGCTAGGCTTACTAGCCCTGGGCTCAAAAGCAATGGAAATGGGAAAAGGCAGGCCAACATGGGCCGTAGTATTGAGCATAGTAGCATTATGTGGGGACGTGGCATTATTTTCAATTGGGCTTGGGCCAATAGCGTGGGTGTACTCATCGGAGATATTCCCGAACAGGATGAGGGCGCAGGGGTCGAGCCTGGCGGTATCAGTGAACAGATTGGTGAGCGGAGTGGTATCGATGACATTTCTGAGCATATCAAAAGAGATCACGTTTGGAGGCATGTTCATGGTGCTATCGGGAATAATGGCACTGGGTACCCTCTTCTTTTACTTCTTTTTGCCAGAGACTAAAGGAAAAAGCTTGGAGGAGATGGAGGTCCTGTTCCAGGACAAGGACACAAACAGTACTACGCCACTGCAGATTGAAGAGACATTGTAGTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTACCTACTACCTTTTCCCTTATTTCTTCATCACTTTATGTTCATGTTTTGCGTAGCATTGTGGATCATCATGGATGAGCTGATGAACTCTCATAGGATCAAAGTTTATTATTTATTATTAAGATGAACATAGCTTAGTTGGGATTAAACTTTTGGGTTGAAAATTTGAAATACTTTGTGGGGGGATGTATGATTGGTCAAGTCAAGGGTGGGAATGAAA

Coding sequence (CDS)

ATGGAAGCCGCTGCTGCAACTCCGATGGAACCCATGGATGTGGAAGAAGCCTCCAAACTGCAACCCAAGGGATTTGGGATCAACAAGTACACCTTAATTTGCTCCATTTTGGCCTCCACCAACTCCATTTTGTTGGGCTACGATATTGGAGTGATGAGTGGGGCTGTGCTCTACATCGAAGAGAATCTCAAAATCTCATCCACCCAAGTGGAAATCCTTGTGGGATCGCTAAACGTACTCTCTCTAGTCGGATCTCTCGCTTCCGGGCGGACCTCTGATTCAATCGGCCGGCGATACACGACATTCCTCGCATCCACCACGTTTCTAATCGGCGCGTTGTTAATGGGTCTCGCCCCATCTTTTCCTCTTCTCCTAGTCGGTCGTATGATCGCTGGCGTCGGCGTCGGTTACGCCCTCATGATCGCCCCTGTCTACACGGCGGAGCTCTCTCCGGCTACATCCCGCGGCCTTCTCAGTTCATTCCCGGAAATCTTCATCACGTTCGGTATTTTACTGGGCTACATCATCAACTACGCTCTGTCCGGCCTACCACCGCACATCAACTGGAGAATCATGCTCGGAATCGCCGGAATACCAGCGCTGGGCGTCGGATTGGGCGTTCTAACAATGCCCGAATCCCCGCGTTGGTTAGTCATGAAGGGCAAATCGGAAGAAGCCAAAAAGGTCTTAATCAAAATCTCCACCAACGAAATCGAAGCAGAGGAGAGACTGAAGTCAATAACCGAAGCCGTAGCCGCAGCCGCGCCCGGCGGCAAATGGAACGGACAAGGGGTTTGGAAGGAACTACTGATAAAACCCACAAAGCCAATCCGCCGAATACTAATAGCCGCCATCGGAATCAACTTCTTCATGCAAGCATCAGGAAACGACGCCGTAATGTACTACTCACCGGAAGTATTCCGCGCAGCCGGAATCCAGCGAAAACGGCATCTGTTCAGCGTGAACGTGGTAATGGGCATTACAAAGACCTGCTTCGTTCTATTCTCAGCACTGTACCTAGACCGATTAGGAAGGCGGCCGCTTTTACTAGTGGGGTCAGCCGGGATGACAGTGGCGCTAGGCTTACTAGCCCTGGGCTCAAAAGCAATGGAAATGGGAAAAGGCAGGCCAACATGGGCCGTAGTATTGAGCATAGTAGCATTATGTGGGGACGTGGCATTATTTTCAATTGGGCTTGGGCCAATAGCGTGGGTGTACTCATCGGAGATATTCCCGAACAGGATGAGGGCGCAGGGGTCGAGCCTGGCGGTATCAGTGAACAGATTGGTGAGCGGAGTGGTATCGATGACATTTCTGAGCATATCAAAAGAGATCACGTTTGGAGGCATGTTCATGGTGCTATCGGGAATAATGGCACTGGGTACCCTCTTCTTTTACTTCTTTTTGCCAGAGACTAAAGGAAAAAGCTTGGAGGAGATGGAGGTCCTGTTCCAGGACAAGGACACAAACAGTACTACGCCACTGCAGATTGAAGAGACATTGTAG

Protein sequence

MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLFQDKDTNSTTPLQIEETL
Homology
BLAST of Carg09853 vs. NCBI nr
Match: KAG6589914.1 (putative polyol transporter 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023583.1 putative polyol transporter 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 946.8 bits (2446), Expect = 7.5e-272
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL
Sbjct: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA
Sbjct: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240

Query: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300
           EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM
Sbjct: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300

Query: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360
           YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL
Sbjct: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360

Query: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420
           GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS
Sbjct: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420

Query: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480
           SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM
Sbjct: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480

Query: 481 EVLFQDKDTNSTTPLQIEETL 502
           EVLFQDKDTNSTTPLQIEETL
Sbjct: 481 EVLFQDKDTNSTTPLQIEETL 501

BLAST of Carg09853 vs. NCBI nr
Match: XP_022987698.1 (probable polyol transporter 6 [Cucurbita maxima])

HSP 1 Score: 942.2 bits (2434), Expect = 1.8e-270
Identity = 497/501 (99.20%), Postives = 500/501 (99.80%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLL GRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL
Sbjct: 121 FPLLLAGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA
Sbjct: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240

Query: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300
           EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM
Sbjct: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300

Query: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360
           YYSPEVFRAAGIQRKRHLFSVNVVMG+TKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL
Sbjct: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGVTKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360

Query: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420
           GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS
Sbjct: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420

Query: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480
           SLAVSVNRLVSGVVSMTFLSIS+EITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM
Sbjct: 421 SLAVSVNRLVSGVVSMTFLSISEEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480

Query: 481 EVLFQDKDTNSTTPLQIEETL 502
           EVLFQDKDTNSTTP+QIEETL
Sbjct: 481 EVLFQDKDTNSTTPVQIEETL 501

BLAST of Carg09853 vs. NCBI nr
Match: XP_022960720.1 (probable polyol transporter 6 [Cucurbita moschata])

HSP 1 Score: 939.9 bits (2428), Expect = 9.2e-270
Identity = 497/501 (99.20%), Postives = 500/501 (99.80%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL
Sbjct: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           SGLP HINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA
Sbjct: 181 SGLPLHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240

Query: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300
           EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM
Sbjct: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300

Query: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360
           YYSPEVFRAAGIQ+KRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL
Sbjct: 301 YYSPEVFRAAGIQQKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360

Query: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420
           GLLALGSKAMEMGKGRPTWAVVLSI+ALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS
Sbjct: 361 GLLALGSKAMEMGKGRPTWAVVLSIIALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420

Query: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480
           SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM
Sbjct: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480

Query: 481 EVLFQDKDTNSTTPLQIEETL 502
           EVLFQDKDTNSTTP+QIEETL
Sbjct: 481 EVLFQDKDTNSTTPVQIEETL 501

BLAST of Carg09853 vs. NCBI nr
Match: XP_023515881.1 (probable polyol transporter 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939.5 bits (2427), Expect = 1.2e-269
Identity = 496/501 (99.00%), Postives = 499/501 (99.60%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           MEAAAATPMEPMDVE ASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 1   MEAAAATPMEPMDVEGASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL
Sbjct: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA
Sbjct: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240

Query: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300
           EERLKSITEA AAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM
Sbjct: 241 EERLKSITEAAAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300

Query: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360
           YYSPEVFRAAGIQ+KRHLFSVNVVMG+TKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL
Sbjct: 301 YYSPEVFRAAGIQQKRHLFSVNVVMGVTKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360

Query: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420
           GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS
Sbjct: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420

Query: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480
           SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM
Sbjct: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480

Query: 481 EVLFQDKDTNSTTPLQIEETL 502
           EVLFQDKDTNSTTP+QIEETL
Sbjct: 481 EVLFQDKDTNSTTPVQIEETL 501

BLAST of Carg09853 vs. NCBI nr
Match: XP_038878586.1 (probable polyol transporter 6 [Benincasa hispida] >XP_038878590.1 probable polyol transporter 6 [Benincasa hispida])

HSP 1 Score: 814.3 bits (2102), Expect = 5.8e-232
Identity = 429/491 (87.37%), Postives = 457/491 (93.08%), Query Frame = 0

Query: 3   AAAATPMEPMDVEE-ASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIEE 62
           A AAT +E ++ EE +SK+   GFGINKYT ICSILASTNSILLGYDIGVMSGAVLYIEE
Sbjct: 15  ATAATAVEAIEEEEGSSKVASMGFGINKYTFICSILASTNSILLGYDIGVMSGAVLYIEE 74

Query: 63  NLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSF 122
           NL ISSTQVEILVGSLNVLSL+GSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS+
Sbjct: 75  NLNISSTQVEILVGSLNVLSLIGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSY 134

Query: 123 PLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYALS 182
           PLLL GRMIAG+GVGYALMIAPVYTAELSPATSRGLLSS PEIFITFGILLGYIINYALS
Sbjct: 135 PLLLAGRMIAGIGVGYALMIAPVYTAELSPATSRGLLSSLPEIFITFGILLGYIINYALS 194

Query: 183 GLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAE 242
            LPPHINWRIMLG+AGIPALGVGLGVL+MPESPRWLVMKGKSEEA+KVL+KISTNEIEAE
Sbjct: 195 DLPPHINWRIMLGLAGIPALGVGLGVLSMPESPRWLVMKGKSEEARKVLLKISTNEIEAE 254

Query: 243 ERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMY 302
           ERL++IT+A AAAA G  W+GQGVWKELLI PTKPIRR+LIAA+GINFFMQASGNDAVMY
Sbjct: 255 ERLRAITDAAAAAAAG--WHGQGVWKELLINPTKPIRRMLIAAVGINFFMQASGNDAVMY 314

Query: 303 YSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALG 362
           YSPEVFRAAGI+ KRHLF VNVVMG+TK  FVLFSALYLDR GRR LLLVGS GM VAL 
Sbjct: 315 YSPEVFRAAGIREKRHLFGVNVVMGVTKISFVLFSALYLDRFGRRRLLLVGSTGMAVALA 374

Query: 363 LLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSS 422
           LL LGSK ME GKGRPTWAVVLSI+ALCGDVALFSIGLGPI WVYSSEIFPNRMRAQGSS
Sbjct: 375 LLGLGSKVMERGKGRPTWAVVLSIIALCGDVALFSIGLGPITWVYSSEIFPNRMRAQGSS 434

Query: 423 LAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEME 482
           LAVSVNRLVSGVVSMTFLSISKEITFGGMF+VLSGIMA+ T+FFYFFLPETKGKSLEEME
Sbjct: 435 LAVSVNRLVSGVVSMTFLSISKEITFGGMFLVLSGIMAVATVFFYFFLPETKGKSLEEME 494

Query: 483 VLFQDKDTNST 493
           +LFQDK++  +
Sbjct: 495 ILFQDKNSKQS 503

BLAST of Carg09853 vs. ExPASy Swiss-Prot
Match: Q8GXR2 (Probable polyol transporter 6 OS=Arabidopsis thaliana OX=3702 GN=PLT6 PE=2 SV=2)

HSP 1 Score: 485.3 bits (1248), Expect = 8.1e-136
Identity = 251/473 (53.07%), Postives = 342/473 (72.30%), Query Frame = 0

Query: 23  KGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSL 82
           K  G+N++ L C+I+AS  SI+ GYD GVMSGA+++IEE+LK +  Q+E+L G LN+ +L
Sbjct: 9   KPAGVNRFALQCAIVASIVSIIFGYDTGVMSGAMVFIEEDLKTNDVQIEVLTGILNLCAL 68

Query: 83  VGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIA 142
           VGSL +GRTSD IGRRYT  LAS  F++G++LMG  P++P+LL GR  AG+GVG+ALM+A
Sbjct: 69  VGSLLAGRTSDIIGRRYTIVLASILFMLGSILMGWGPNYPVLLSGRCTAGLGVGFALMVA 128

Query: 143 PVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALG 202
           PVY+AE++ A+ RGLL+S P + I+ GILLGYI+NY  S LP HI WR+MLGIA +P+L 
Sbjct: 129 PVYSAEIATASHRGLLASLPHLCISIGILLGYIVNYFFSKLPMHIGWRLMLGIAAVPSLV 188

Query: 203 VGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAAAAP------ 262
           +  G+L MPESPRWL+M+G+ +E K++L  +S +  EAE R + I +A A   P      
Sbjct: 189 LAFGILKMPESPRWLIMQGRLKEGKEILELVSNSPEEAELRFQDI-KAAAGIDPKCVDDV 248

Query: 263 ----GGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGI 322
               G K +G+GVWKEL+++PT  +RR+L+ A+GI+FF  ASG +AV+ Y P +F+ AGI
Sbjct: 249 VKMEGKKTHGEGVWKELILRPTPAVRRVLLTALGIHFFQHASGIEAVLLYGPRIFKKAGI 308

Query: 323 QRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEM 382
             K  LF V + +GI KT F+  + L LD++GRR LLL    GM +AL +L  G    + 
Sbjct: 309 TTKDKLFLVTIGVGIMKTTFIFTATLLLDKVGRRKLLLTSVGGMVIALTMLGFGLTMAQN 368

Query: 383 GKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSG 442
             G+  WA+VLSIVA    VA FSIGLGPI WVYSSE+FP ++RAQG+SL V+VNR+++ 
Sbjct: 369 AGGKLAWALVLSIVAAYSFVAFFSIGLGPITWVYSSEVFPLKLRAQGASLGVAVNRVMNA 428

Query: 443 VVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLFQ 486
            VSM+FLS++  IT GG F + +G+ A+   FF+F LPETKGKSLEE+E LFQ
Sbjct: 429 TVSMSFLSLTSAITTGGAFFMFAGVAAVAWNFFFFLLPETKGKSLEEIEALFQ 480

BLAST of Carg09853 vs. ExPASy Swiss-Prot
Match: Q8VZ80 (Polyol transporter 5 OS=Arabidopsis thaliana OX=3702 GN=PLT5 PE=1 SV=2)

HSP 1 Score: 479.9 bits (1234), Expect = 3.4e-134
Identity = 250/466 (53.65%), Postives = 329/466 (70.60%), Query Frame = 0

Query: 28  NKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSLA 87
           N Y   C+ILAS  SILLGYDIGVMSGA++YI+ +LKI+  Q+ IL GSLN+ SL+GS A
Sbjct: 33  NNYAFACAILASMTSILLGYDIGVMSGAMIYIKRDLKINDLQIGILAGSLNIYSLIGSCA 92

Query: 88  SGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYTA 147
           +GRTSD IGRRYT  LA   F  GA+LMGL+P++  L+ GR IAG+GVGYALMIAPVYTA
Sbjct: 93  AGRTSDWIGRRYTIVLAGAIFFAGAILMGLSPNYAFLMFGRFIAGIGVGYALMIAPVYTA 152

Query: 148 ELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLGV 207
           E+SPA+SRG L+SFPE+FI  GI+LGY+ N A S LP  + WR+MLGI  +P++ + +GV
Sbjct: 153 EVSPASSRGFLNSFPEVFINAGIMLGYVSNLAFSNLPLKVGWRLMLGIGAVPSVILAIGV 212

Query: 208 LTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAAAA---------PGG 267
           L MPESPRWLVM+G+  +AK+VL K S +  EA  RL+ I  A    A            
Sbjct: 213 LAMPESPRWLVMQGRLGDAKRVLDKTSDSPTEATLRLEDIKHAAGIPADCHDDVVQVSRR 272

Query: 268 KWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRHL 327
             +G+GVW+ELLI+PT  +RR++IAAIGI+FF QASG DAV+ +SP +F+ AG++     
Sbjct: 273 NSHGEGVWRELLIRPTPAVRRVMIAAIGIHFFQQASGIDAVVLFSPRIFKTAGLKTDHQQ 332

Query: 328 FSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRPT 387
               V +G+ KT F+L +   LDR+GRRPLLL    GM ++L  L      ++  + +  
Sbjct: 333 LLATVAVGVVKTSFILVATFLLDRIGRRPLLLTSVGGMVLSLAALGTSLTIIDQSEKKVM 392

Query: 388 WAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMTF 447
           WAVV++I  +   VA FSIG GPI WVYSSEIFP R+R+QGSS+ V VNR+ SGV+S++F
Sbjct: 393 WAVVVAIATVMTYVATFSIGAGPITWVYSSEIFPLRLRSQGSSMGVVVNRVTSGVISISF 452

Query: 448 LSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLF 485
           L +SK +T GG F +  GI  +  +FFY FLPET+G+ LE+M+ LF
Sbjct: 453 LPMSKAMTTGGAFYLFGGIATVAWVFFYTFLPETQGRMLEDMDELF 498

BLAST of Carg09853 vs. ExPASy Swiss-Prot
Match: Q9XIH7 (Putative polyol transporter 1 OS=Arabidopsis thaliana OX=3702 GN=PLT1 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 3.0e-130
Identity = 245/473 (51.80%), Postives = 324/473 (68.50%), Query Frame = 0

Query: 28  NKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSLA 87
           ++Y   C+ILAS  SI+LGYDIGVMSGA ++I+++LK+S  Q+EIL+G LN+ SLVGS A
Sbjct: 23  SRYAFACAILASMTSIILGYDIGVMSGASIFIKDDLKLSDVQLEILMGILNIYSLVGSGA 82

Query: 88  SGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYTA 147
           +GRTSD +GRRYT  LA   F  GALLMG A ++P ++VGR +AG+GVGYA+MIAPVYTA
Sbjct: 83  AGRTSDWLGRRYTIVLAGAFFFCGALLMGFATNYPFIMVGRFVAGIGVGYAMMIAPVYTA 142

Query: 148 ELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLGV 207
           E++PA+SRG L+SFPEIFI  GILLGY+ NY  S LP H+ WR MLG+  +P++ + +GV
Sbjct: 143 EVAPASSRGFLTSFPEIFINIGILLGYVSNYFFSKLPEHLGWRFMLGVGAVPSVFLAIGV 202

Query: 208 LTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAV---------AAAAPGG 267
           L MPESPRWLV++G+  +A KVL K S  + EA  RL  I  AV             P  
Sbjct: 203 LAMPESPRWLVLQGRLGDAFKVLDKTSNTKEEAISRLDDIKRAVGIPDDMTDDVIVVPNK 262

Query: 268 KWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRHL 327
           K  G+GVWK+LL++PT  +R ILIA +GI+F  QASG DAV+ YSP +F  AG++ K   
Sbjct: 263 KSAGKGVWKDLLVRPTPSVRHILIACLGIHFAQQASGIDAVVLYSPTIFSKAGLKSKNDQ 322

Query: 328 FSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRP- 387
               V +G+ KT F++     +DR GRR LLL    GM ++L  L      +    G+  
Sbjct: 323 LLATVAVGVVKTLFIVVGTCVVDRFGRRALLLTSMGGMFLSLTALGTSLTVINRNPGQTL 382

Query: 388 TWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMT 447
            WA+ L++  +   VA FSIG GP+ WVY SEIFP R+RAQG+SL V +NRL+SG++ MT
Sbjct: 383 KWAIGLAVTTVMTFVATFSIGAGPVTWVYCSEIFPVRLRAQGASLGVMLNRLMSGIIGMT 442

Query: 448 FLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLFQDKDTN 491
           FLS+SK +T GG F++ +G+ A   +FF+ FLPET+G  LEEME LF     N
Sbjct: 443 FLSLSKGLTIGGAFLLFAGVAAAAWVFFFTFLPETRGIPLEEMETLFGSYTAN 495

BLAST of Carg09853 vs. ExPASy Swiss-Prot
Match: Q9XIH6 (Putative polyol transporter 2 OS=Arabidopsis thaliana OX=3702 GN=PLT2 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.5e-129
Identity = 248/491 (50.51%), Postives = 329/491 (67.01%), Query Frame = 0

Query: 10  EPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQ 69
           E   V  A    P+G   +++   C+ILAS  SI+LGYDIGVMSGA ++I+++LK+S  Q
Sbjct: 6   EERGVVVAESEPPRG-NRSRFAFACAILASMTSIILGYDIGVMSGAAIFIKDDLKLSDVQ 65

Query: 70  VEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRM 129
           +EIL+G LN+ SL+GS A+GRTSD IGRRYT  LA   F  GALLMG A ++P ++VGR 
Sbjct: 66  LEILMGILNIYSLIGSGAAGRTSDWIGRRYTIVLAGFFFFCGALLMGFATNYPFIMVGRF 125

Query: 130 IAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINW 189
           +AG+GVGYA+MIAPVYT E++PA+SRG LSSFPEIFI  GILLGY+ NY  + LP HI W
Sbjct: 126 VAGIGVGYAMMIAPVYTTEVAPASSRGFLSSFPEIFINIGILLGYVSNYFFAKLPEHIGW 185

Query: 190 RIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITE 249
           R MLGI  +P++ + +GVL MPESPRWLVM+G+  +A KVL K S  + EA  RL  I  
Sbjct: 186 RFMLGIGAVPSVFLAIGVLAMPESPRWLVMQGRLGDAFKVLDKTSNTKEEAISRLNDIKR 245

Query: 250 AV---------AAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 309
           AV             P  K  G+GVWK+LL++PT  +R ILIA +GI+F  QASG DAV+
Sbjct: 246 AVGIPDDMTDDVIVVPNKKSAGKGVWKDLLVRPTPSVRHILIACLGIHFSQQASGIDAVV 305

Query: 310 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 369
            YSP +F  AG++ K       V +G+ KT F++     +DR GRR LLL    GM  +L
Sbjct: 306 LYSPTIFSRAGLKSKNDQLLATVAVGVVKTLFIVVGTCLVDRFGRRALLLTSMGGMFFSL 365

Query: 370 GLLALGSKAMEMGKGRP-TWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQG 429
             L      ++   G+   WA+ L++  +   VA FS+G GP+ WVY+SEIFP R+RAQG
Sbjct: 366 TALGTSLTVIDRNPGQTLKWAIGLAVTTVMTFVATFSLGAGPVTWVYASEIFPVRLRAQG 425

Query: 430 SSLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEE 489
           +SL V +NRL+SG++ MTFLS+SK +T GG F++ +G+     +FF+ FLPET+G  LEE
Sbjct: 426 ASLGVMLNRLMSGIIGMTFLSLSKGLTIGGAFLLFAGVAVAAWVFFFTFLPETRGVPLEE 485

Query: 490 MEVLFQDKDTN 491
           +E LF     N
Sbjct: 486 IESLFGSYSAN 495

BLAST of Carg09853 vs. ExPASy Swiss-Prot
Match: Q9ZNS0 (Probable polyol transporter 3 OS=Arabidopsis thaliana OX=3702 GN=PLT3 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.3e-128
Identity = 248/467 (53.10%), Postives = 330/467 (70.66%), Query Frame = 0

Query: 27  INKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSL 86
           +NK+   C+I+AS  SI+ GYD GVMSGA ++I ++LKI+ TQ+E+L G LN+ +LVGSL
Sbjct: 18  MNKFAFGCAIVASIISIIFGYDTGVMSGAQIFIRDDLKINDTQIEVLAGILNLCALVGSL 77

Query: 87  ASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYT 146
            +G+TSD IGRRYT  L++  FL+G++LMG  P++P+L+VGR IAGVGVG+ALMIAPVY+
Sbjct: 78  TAGKTSDVIGRRYTIALSAVIFLVGSVLMGYGPNYPVLMVGRCIAGVGVGFALMIAPVYS 137

Query: 147 AELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLG 206
           AE+S A+ RG L+S PE+ I+ GILLGY+ NY    L   + WR+MLGIA  P+L +  G
Sbjct: 138 AEISSASHRGFLTSLPELCISLGILLGYVSNYCFGKLTLKLGWRLMLGIAAFPSLILAFG 197

Query: 207 VLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAA------AAPGG-- 266
           +  MPESPRWLVM+G+ EEAKK+++ +S  E EAEER + I  A            GG  
Sbjct: 198 ITRMPESPRWLVMQGRLEEAKKIMVLVSNTEEEAEERFRDILTAAEVDVTEIKEVGGGVK 257

Query: 267 -KWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRH 326
            K +G+ VW+EL+IKP   +R ILIAA+GI+FF  A+G +AV+ YSP +F+ AG+  K  
Sbjct: 258 KKNHGKSVWRELVIKPRPAVRLILIAAVGIHFFEHATGIEAVVLYSPRIFKKAGVVSKDK 317

Query: 327 LFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRP 386
           L    V +G+TK  F++ +   LD++GRR LLL  + GM  AL  LA+ S  M    GR 
Sbjct: 318 LLLATVGVGLTKAFFIIIATFLLDKVGRRKLLLTSTGGMVFALTSLAV-SLTMVQRFGRL 377

Query: 387 TWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMT 446
            WA+ LSIV+    VA FSIGLGPI WVYSSEIFP R+RAQG+S+ V+VNR+++  VSM+
Sbjct: 378 AWALSLSIVSTYAFVAFFSIGLGPITWVYSSEIFPLRLRAQGASIGVAVNRIMNATVSMS 437

Query: 447 FLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLF 485
           FLS++K IT GG+F V +GI      FF+F LPETKG  LEEME LF
Sbjct: 438 FLSMTKAITTGGVFFVFAGIAVAAWWFFFFMLPETKGLPLEEMEKLF 483

BLAST of Carg09853 vs. ExPASy TrEMBL
Match: A0A6J1JK73 (probable polyol transporter 6 OS=Cucurbita maxima OX=3661 GN=LOC111485176 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 8.9e-271
Identity = 497/501 (99.20%), Postives = 500/501 (99.80%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLL GRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL
Sbjct: 121 FPLLLAGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA
Sbjct: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240

Query: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300
           EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM
Sbjct: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300

Query: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360
           YYSPEVFRAAGIQRKRHLFSVNVVMG+TKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL
Sbjct: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGVTKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360

Query: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420
           GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS
Sbjct: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420

Query: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480
           SLAVSVNRLVSGVVSMTFLSIS+EITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM
Sbjct: 421 SLAVSVNRLVSGVVSMTFLSISEEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480

Query: 481 EVLFQDKDTNSTTPLQIEETL 502
           EVLFQDKDTNSTTP+QIEETL
Sbjct: 481 EVLFQDKDTNSTTPVQIEETL 501

BLAST of Carg09853 vs. ExPASy TrEMBL
Match: A0A6J1H9W5 (probable polyol transporter 6 OS=Cucurbita moschata OX=3662 GN=LOC111461437 PE=3 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.4e-270
Identity = 497/501 (99.20%), Postives = 500/501 (99.80%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL
Sbjct: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           SGLP HINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA
Sbjct: 181 SGLPLHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240

Query: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300
           EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM
Sbjct: 241 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 300

Query: 301 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360
           YYSPEVFRAAGIQ+KRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL
Sbjct: 301 YYSPEVFRAAGIQQKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 360

Query: 361 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420
           GLLALGSKAMEMGKGRPTWAVVLSI+ALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS
Sbjct: 361 GLLALGSKAMEMGKGRPTWAVVLSIIALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 420

Query: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480
           SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM
Sbjct: 421 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 480

Query: 481 EVLFQDKDTNSTTPLQIEETL 502
           EVLFQDKDTNSTTP+QIEETL
Sbjct: 481 EVLFQDKDTNSTTPVQIEETL 501

BLAST of Carg09853 vs. ExPASy TrEMBL
Match: A0A6J1CTF7 (probable polyol transporter 6 OS=Momordica charantia OX=3673 GN=LOC111014424 PE=3 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.4e-231
Identity = 424/497 (85.31%), Postives = 457/497 (91.95%), Query Frame = 0

Query: 1   MEAAAATPMEPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 60
           ME ++AT ME +++E+ SK Q KGFGINKYTLICS+LASTNSILLGYDIGVMSGAVLYIE
Sbjct: 16  METSSATAMEAIELEKDSKSQSKGFGINKYTLICSVLASTNSILLGYDIGVMSGAVLYIE 75

Query: 61  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 120
           +NLKISSTQVEILVGSLNVLSL+GSLASGRTSDSIGRRYTTFLASTTFL+GALLMGLAPS
Sbjct: 76  DNLKISSTQVEILVGSLNVLSLIGSLASGRTSDSIGRRYTTFLASTTFLVGALLMGLAPS 135

Query: 121 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 180
           FPLLL GRMIAGVGVGYALM+APVYTAELSPATSRGLLSS PEIFIT GILLGYIINYAL
Sbjct: 136 FPLLLAGRMIAGVGVGYALMVAPVYTAELSPATSRGLLSSLPEIFITLGILLGYIINYAL 195

Query: 181 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 240
           S LPPH+NWRIMLG+AG+PAL VG+GVL MPESPRWLVMKGK EEAKKVLIKIS NE EA
Sbjct: 196 SDLPPHMNWRIMLGLAGVPALVVGVGVLAMPESPRWLVMKGKLEEAKKVLIKISINETEA 255

Query: 241 EERLKSITEA----VAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGN 300
           EERL++I+ A     AAAA  G W+GQGVWKELLI PTKPIRR+LIAAIGINFFMQASGN
Sbjct: 256 EERLEAISTAADADAAAAAASGNWHGQGVWKELLINPTKPIRRMLIAAIGINFFMQASGN 315

Query: 301 DAVMYYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGM 360
           DAVMYYSPEVFRAAGI RKRHLF VNVVMG+TKTCFVLFSALYLDR GRRPLLLVGS+GM
Sbjct: 316 DAVMYYSPEVFRAAGIHRKRHLFGVNVVMGVTKTCFVLFSALYLDRFGRRPLLLVGSSGM 375

Query: 361 TVALGLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMR 420
           TVALGLL LGSK ME    RPTWAVV+SIVALCGDVALFSIGLGPI WVY++EIFPNR+R
Sbjct: 376 TVALGLLGLGSKCMEKANSRPTWAVVVSIVALCGDVALFSIGLGPITWVYTTEIFPNRIR 435

Query: 421 AQGSSLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKS 480
           AQGSSLA+S+NRLVSGVVSMTFLSISKEITFGGMF VLSGIMA+ TLFFYFFLPETKGKS
Sbjct: 436 AQGSSLAISLNRLVSGVVSMTFLSISKEITFGGMFFVLSGIMAMATLFFYFFLPETKGKS 495

Query: 481 LEEMEVLFQDKDTNSTT 494
           LEE+E+LF D  T++TT
Sbjct: 496 LEEIELLFHDNHTHTTT 512

BLAST of Carg09853 vs. ExPASy TrEMBL
Match: A0A5A7TX61 (Putative polyol transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00060 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 423/502 (84.26%), Postives = 454/502 (90.44%), Query Frame = 0

Query: 2   EAAAATPMEPMDVEEAS-KLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 61
           EA A   +E ++ EE S  +Q  GFGINKYTL CSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 12  EATAVPAVEAIEEEEQSLGMQSMGFGINKYTLFCSILASTNSILLGYDIGVMSGAVLYIE 71

Query: 62  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 121
           +NLKISSTQVEILVGSLN+LSL+GSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 72  DNLKISSTQVEILVGSLNILSLIGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 131

Query: 122 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 181
           +PLLL GRMIAG+GVGYALM+APVYTAELSP+TSRGLLSS PEIFITFGILLGYIINYAL
Sbjct: 132 YPLLLAGRMIAGIGVGYALMVAPVYTAELSPSTSRGLLSSLPEIFITFGILLGYIINYAL 191

Query: 182 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 241
           SGLPPHINWRIMLG+AGIPA+ VGLGVLTMPESPRWL+MKGKSEEAKKVL+KIS+NEIEA
Sbjct: 192 SGLPPHINWRIMLGLAGIPAIAVGLGVLTMPESPRWLIMKGKSEEAKKVLLKISSNEIEA 251

Query: 242 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 301
           EERL+SI+    AAA G  W+GQGVWKELLI PTKPIRR+LIAAIGINFFMQASGNDAVM
Sbjct: 252 EERLRSIS---GAAAAGSSWHGQGVWKELLINPTKPIRRMLIAAIGINFFMQASGNDAVM 311

Query: 302 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 361
           YYSPEVFRAAGI  KRHLF VNVVMGITKTCFV+ SA+YLDR GRRPLLLVGS GMTVAL
Sbjct: 312 YYSPEVFRAAGIHEKRHLFGVNVVMGITKTCFVMLSAVYLDRFGRRPLLLVGSIGMTVAL 371

Query: 362 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 421
            LL LGSK  + GKGRP W V LS++ALCGDVALFSIGLGPI WVYSSEIFPNRMRAQGS
Sbjct: 372 ALLGLGSKVTKEGKGRPRWGVALSVIALCGDVALFSIGLGPITWVYSSEIFPNRMRAQGS 431

Query: 422 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 481
           SLA+SVNRLVSG+VSMTFLSISKEITFGGMF+VLSGIM + +LFFYFFLPETKGKSLEEM
Sbjct: 432 SLAISVNRLVSGIVSMTFLSISKEITFGGMFLVLSGIMGVASLFFYFFLPETKGKSLEEM 491

Query: 482 EVLFQDKDTNSTT----PLQIE 499
           EVLFQDK  NS T     LQ+E
Sbjct: 492 EVLFQDKVNNSKTDSNNSLQVE 510

BLAST of Carg09853 vs. ExPASy TrEMBL
Match: A0A1S3CKQ3 (probable polyol transporter 6 OS=Cucumis melo OX=3656 GN=LOC103501559 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 423/502 (84.26%), Postives = 454/502 (90.44%), Query Frame = 0

Query: 2   EAAAATPMEPMDVEEAS-KLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIE 61
           EA A   +E ++ EE S  +Q  GFGINKYTL CSILASTNSILLGYDIGVMSGAVLYIE
Sbjct: 12  EATAVPAVEAIEEEEQSLGMQSMGFGINKYTLFCSILASTNSILLGYDIGVMSGAVLYIE 71

Query: 62  ENLKISSTQVEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 121
           +NLKISSTQVEILVGSLN+LSL+GSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS
Sbjct: 72  DNLKISSTQVEILVGSLNILSLIGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPS 131

Query: 122 FPLLLVGRMIAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYAL 181
           +PLLL GRMIAG+GVGYALM+APVYTAELSP+TSRGLLSS PEIFITFGILLGYIINYAL
Sbjct: 132 YPLLLAGRMIAGIGVGYALMVAPVYTAELSPSTSRGLLSSLPEIFITFGILLGYIINYAL 191

Query: 182 SGLPPHINWRIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEA 241
           SGLPPHINWRIMLG+AGIPA+ VGLGVLTMPESPRWL+MKGKSEEAKKVL+KIS+NEIEA
Sbjct: 192 SGLPPHINWRIMLGLAGIPAIAVGLGVLTMPESPRWLIMKGKSEEAKKVLLKISSNEIEA 251

Query: 242 EERLKSITEAVAAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 301
           EERL+SI+    AAA G  W+GQGVWKELLI PTKPIRR+LIAAIGINFFMQASGNDAVM
Sbjct: 252 EERLRSIS---GAAAAGSSWHGQGVWKELLINPTKPIRRMLIAAIGINFFMQASGNDAVM 311

Query: 302 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 361
           YYSPEVFRAAGI  KRHLF VNVVMGITKTCFV+ SA+YLDR GRRPLLLVGS GMTVAL
Sbjct: 312 YYSPEVFRAAGIHEKRHLFGVNVVMGITKTCFVMLSAVYLDRFGRRPLLLVGSIGMTVAL 371

Query: 362 GLLALGSKAMEMGKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGS 421
            LL LGSK  + GKGRP W V LS++ALCGDVALFSIGLGPI WVYSSEIFPNRMRAQGS
Sbjct: 372 ALLGLGSKVTKEGKGRPRWGVALSVIALCGDVALFSIGLGPITWVYSSEIFPNRMRAQGS 431

Query: 422 SLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEM 481
           SLA+SVNRLVSG+VSMTFLSISKEITFGGMF+VLSGIM + +LFFYFFLPETKGKSLEEM
Sbjct: 432 SLAISVNRLVSGIVSMTFLSISKEITFGGMFLVLSGIMGVASLFFYFFLPETKGKSLEEM 491

Query: 482 EVLFQDKDTNSTT----PLQIE 499
           EVLFQDK  NS T     LQ+E
Sbjct: 492 EVLFQDKVNNSKTDSNNSLQVE 510

BLAST of Carg09853 vs. TAIR 10
Match: AT4G36670.1 (Major facilitator superfamily protein )

HSP 1 Score: 485.3 bits (1248), Expect = 5.7e-137
Identity = 251/473 (53.07%), Postives = 342/473 (72.30%), Query Frame = 0

Query: 23  KGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSL 82
           K  G+N++ L C+I+AS  SI+ GYD GVMSGA+++IEE+LK +  Q+E+L G LN+ +L
Sbjct: 9   KPAGVNRFALQCAIVASIVSIIFGYDTGVMSGAMVFIEEDLKTNDVQIEVLTGILNLCAL 68

Query: 83  VGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIA 142
           VGSL +GRTSD IGRRYT  LAS  F++G++LMG  P++P+LL GR  AG+GVG+ALM+A
Sbjct: 69  VGSLLAGRTSDIIGRRYTIVLASILFMLGSILMGWGPNYPVLLSGRCTAGLGVGFALMVA 128

Query: 143 PVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALG 202
           PVY+AE++ A+ RGLL+S P + I+ GILLGYI+NY  S LP HI WR+MLGIA +P+L 
Sbjct: 129 PVYSAEIATASHRGLLASLPHLCISIGILLGYIVNYFFSKLPMHIGWRLMLGIAAVPSLV 188

Query: 203 VGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAAAAP------ 262
           +  G+L MPESPRWL+M+G+ +E K++L  +S +  EAE R + I +A A   P      
Sbjct: 189 LAFGILKMPESPRWLIMQGRLKEGKEILELVSNSPEEAELRFQDI-KAAAGIDPKCVDDV 248

Query: 263 ----GGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGI 322
               G K +G+GVWKEL+++PT  +RR+L+ A+GI+FF  ASG +AV+ Y P +F+ AGI
Sbjct: 249 VKMEGKKTHGEGVWKELILRPTPAVRRVLLTALGIHFFQHASGIEAVLLYGPRIFKKAGI 308

Query: 323 QRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEM 382
             K  LF V + +GI KT F+  + L LD++GRR LLL    GM +AL +L  G    + 
Sbjct: 309 TTKDKLFLVTIGVGIMKTTFIFTATLLLDKVGRRKLLLTSVGGMVIALTMLGFGLTMAQN 368

Query: 383 GKGRPTWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSG 442
             G+  WA+VLSIVA    VA FSIGLGPI WVYSSE+FP ++RAQG+SL V+VNR+++ 
Sbjct: 369 AGGKLAWALVLSIVAAYSFVAFFSIGLGPITWVYSSEVFPLKLRAQGASLGVAVNRVMNA 428

Query: 443 VVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLFQ 486
            VSM+FLS++  IT GG F + +G+ A+   FF+F LPETKGKSLEE+E LFQ
Sbjct: 429 TVSMSFLSLTSAITTGGAFFMFAGVAAVAWNFFFFLLPETKGKSLEEIEALFQ 480

BLAST of Carg09853 vs. TAIR 10
Match: AT3G18830.1 (polyol/monosaccharide transporter 5 )

HSP 1 Score: 479.9 bits (1234), Expect = 2.4e-135
Identity = 250/466 (53.65%), Postives = 329/466 (70.60%), Query Frame = 0

Query: 28  NKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSLA 87
           N Y   C+ILAS  SILLGYDIGVMSGA++YI+ +LKI+  Q+ IL GSLN+ SL+GS A
Sbjct: 33  NNYAFACAILASMTSILLGYDIGVMSGAMIYIKRDLKINDLQIGILAGSLNIYSLIGSCA 92

Query: 88  SGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYTA 147
           +GRTSD IGRRYT  LA   F  GA+LMGL+P++  L+ GR IAG+GVGYALMIAPVYTA
Sbjct: 93  AGRTSDWIGRRYTIVLAGAIFFAGAILMGLSPNYAFLMFGRFIAGIGVGYALMIAPVYTA 152

Query: 148 ELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLGV 207
           E+SPA+SRG L+SFPE+FI  GI+LGY+ N A S LP  + WR+MLGI  +P++ + +GV
Sbjct: 153 EVSPASSRGFLNSFPEVFINAGIMLGYVSNLAFSNLPLKVGWRLMLGIGAVPSVILAIGV 212

Query: 208 LTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAAAA---------PGG 267
           L MPESPRWLVM+G+  +AK+VL K S +  EA  RL+ I  A    A            
Sbjct: 213 LAMPESPRWLVMQGRLGDAKRVLDKTSDSPTEATLRLEDIKHAAGIPADCHDDVVQVSRR 272

Query: 268 KWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRHL 327
             +G+GVW+ELLI+PT  +RR++IAAIGI+FF QASG DAV+ +SP +F+ AG++     
Sbjct: 273 NSHGEGVWRELLIRPTPAVRRVMIAAIGIHFFQQASGIDAVVLFSPRIFKTAGLKTDHQQ 332

Query: 328 FSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRPT 387
               V +G+ KT F+L +   LDR+GRRPLLL    GM ++L  L      ++  + +  
Sbjct: 333 LLATVAVGVVKTSFILVATFLLDRIGRRPLLLTSVGGMVLSLAALGTSLTIIDQSEKKVM 392

Query: 388 WAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMTF 447
           WAVV++I  +   VA FSIG GPI WVYSSEIFP R+R+QGSS+ V VNR+ SGV+S++F
Sbjct: 393 WAVVVAIATVMTYVATFSIGAGPITWVYSSEIFPLRLRSQGSSMGVVVNRVTSGVISISF 452

Query: 448 LSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLF 485
           L +SK +T GG F +  GI  +  +FFY FLPET+G+ LE+M+ LF
Sbjct: 453 LPMSKAMTTGGAFYLFGGIATVAWVFFYTFLPETQGRMLEDMDELF 498

BLAST of Carg09853 vs. TAIR 10
Match: AT2G16120.1 (polyol/monosaccharide transporter 1 )

HSP 1 Score: 466.8 bits (1200), Expect = 2.1e-131
Identity = 245/473 (51.80%), Postives = 324/473 (68.50%), Query Frame = 0

Query: 28  NKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSLA 87
           ++Y   C+ILAS  SI+LGYDIGVMSGA ++I+++LK+S  Q+EIL+G LN+ SLVGS A
Sbjct: 23  SRYAFACAILASMTSIILGYDIGVMSGASIFIKDDLKLSDVQLEILMGILNIYSLVGSGA 82

Query: 88  SGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYTA 147
           +GRTSD +GRRYT  LA   F  GALLMG A ++P ++VGR +AG+GVGYA+MIAPVYTA
Sbjct: 83  AGRTSDWLGRRYTIVLAGAFFFCGALLMGFATNYPFIMVGRFVAGIGVGYAMMIAPVYTA 142

Query: 148 ELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLGV 207
           E++PA+SRG L+SFPEIFI  GILLGY+ NY  S LP H+ WR MLG+  +P++ + +GV
Sbjct: 143 EVAPASSRGFLTSFPEIFINIGILLGYVSNYFFSKLPEHLGWRFMLGVGAVPSVFLAIGV 202

Query: 208 LTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAV---------AAAAPGG 267
           L MPESPRWLV++G+  +A KVL K S  + EA  RL  I  AV             P  
Sbjct: 203 LAMPESPRWLVLQGRLGDAFKVLDKTSNTKEEAISRLDDIKRAVGIPDDMTDDVIVVPNK 262

Query: 268 KWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRHL 327
           K  G+GVWK+LL++PT  +R ILIA +GI+F  QASG DAV+ YSP +F  AG++ K   
Sbjct: 263 KSAGKGVWKDLLVRPTPSVRHILIACLGIHFAQQASGIDAVVLYSPTIFSKAGLKSKNDQ 322

Query: 328 FSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRP- 387
               V +G+ KT F++     +DR GRR LLL    GM ++L  L      +    G+  
Sbjct: 323 LLATVAVGVVKTLFIVVGTCVVDRFGRRALLLTSMGGMFLSLTALGTSLTVINRNPGQTL 382

Query: 388 TWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMT 447
            WA+ L++  +   VA FSIG GP+ WVY SEIFP R+RAQG+SL V +NRL+SG++ MT
Sbjct: 383 KWAIGLAVTTVMTFVATFSIGAGPVTWVYCSEIFPVRLRAQGASLGVMLNRLMSGIIGMT 442

Query: 448 FLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLFQDKDTN 491
           FLS+SK +T GG F++ +G+ A   +FF+ FLPET+G  LEEME LF     N
Sbjct: 443 FLSLSKGLTIGGAFLLFAGVAAAAWVFFFTFLPETRGIPLEEMETLFGSYTAN 495

BLAST of Carg09853 vs. TAIR 10
Match: AT2G16130.1 (polyol/monosaccharide transporter 2 )

HSP 1 Score: 464.5 bits (1194), Expect = 1.1e-130
Identity = 248/491 (50.51%), Postives = 329/491 (67.01%), Query Frame = 0

Query: 10  EPMDVEEASKLQPKGFGINKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQ 69
           E   V  A    P+G   +++   C+ILAS  SI+LGYDIGVMSGA ++I+++LK+S  Q
Sbjct: 6   EERGVVVAESEPPRG-NRSRFAFACAILASMTSIILGYDIGVMSGAAIFIKDDLKLSDVQ 65

Query: 70  VEILVGSLNVLSLVGSLASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRM 129
           +EIL+G LN+ SL+GS A+GRTSD IGRRYT  LA   F  GALLMG A ++P ++VGR 
Sbjct: 66  LEILMGILNIYSLIGSGAAGRTSDWIGRRYTIVLAGFFFFCGALLMGFATNYPFIMVGRF 125

Query: 130 IAGVGVGYALMIAPVYTAELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINW 189
           +AG+GVGYA+MIAPVYT E++PA+SRG LSSFPEIFI  GILLGY+ NY  + LP HI W
Sbjct: 126 VAGIGVGYAMMIAPVYTTEVAPASSRGFLSSFPEIFINIGILLGYVSNYFFAKLPEHIGW 185

Query: 190 RIMLGIAGIPALGVGLGVLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITE 249
           R MLGI  +P++ + +GVL MPESPRWLVM+G+  +A KVL K S  + EA  RL  I  
Sbjct: 186 RFMLGIGAVPSVFLAIGVLAMPESPRWLVMQGRLGDAFKVLDKTSNTKEEAISRLNDIKR 245

Query: 250 AV---------AAAAPGGKWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVM 309
           AV             P  K  G+GVWK+LL++PT  +R ILIA +GI+F  QASG DAV+
Sbjct: 246 AVGIPDDMTDDVIVVPNKKSAGKGVWKDLLVRPTPSVRHILIACLGIHFSQQASGIDAVV 305

Query: 310 YYSPEVFRAAGIQRKRHLFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVAL 369
            YSP +F  AG++ K       V +G+ KT F++     +DR GRR LLL    GM  +L
Sbjct: 306 LYSPTIFSRAGLKSKNDQLLATVAVGVVKTLFIVVGTCLVDRFGRRALLLTSMGGMFFSL 365

Query: 370 GLLALGSKAMEMGKGRP-TWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQG 429
             L      ++   G+   WA+ L++  +   VA FS+G GP+ WVY+SEIFP R+RAQG
Sbjct: 366 TALGTSLTVIDRNPGQTLKWAIGLAVTTVMTFVATFSLGAGPVTWVYASEIFPVRLRAQG 425

Query: 430 SSLAVSVNRLVSGVVSMTFLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEE 489
           +SL V +NRL+SG++ MTFLS+SK +T GG F++ +G+     +FF+ FLPET+G  LEE
Sbjct: 426 ASLGVMLNRLMSGIIGMTFLSLSKGLTIGGAFLLFAGVAVAAWVFFFTFLPETRGVPLEE 485

Query: 490 MEVLFQDKDTN 491
           +E LF     N
Sbjct: 486 IESLFGSYSAN 495

BLAST of Carg09853 vs. TAIR 10
Match: AT2G18480.1 (Major facilitator superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 8.9e-130
Identity = 248/467 (53.10%), Postives = 330/467 (70.66%), Query Frame = 0

Query: 27  INKYTLICSILASTNSILLGYDIGVMSGAVLYIEENLKISSTQVEILVGSLNVLSLVGSL 86
           +NK+   C+I+AS  SI+ GYD GVMSGA ++I ++LKI+ TQ+E+L G LN+ +LVGSL
Sbjct: 18  MNKFAFGCAIVASIISIIFGYDTGVMSGAQIFIRDDLKINDTQIEVLAGILNLCALVGSL 77

Query: 87  ASGRTSDSIGRRYTTFLASTTFLIGALLMGLAPSFPLLLVGRMIAGVGVGYALMIAPVYT 146
            +G+TSD IGRRYT  L++  FL+G++LMG  P++P+L+VGR IAGVGVG+ALMIAPVY+
Sbjct: 78  TAGKTSDVIGRRYTIALSAVIFLVGSVLMGYGPNYPVLMVGRCIAGVGVGFALMIAPVYS 137

Query: 147 AELSPATSRGLLSSFPEIFITFGILLGYIINYALSGLPPHINWRIMLGIAGIPALGVGLG 206
           AE+S A+ RG L+S PE+ I+ GILLGY+ NY    L   + WR+MLGIA  P+L +  G
Sbjct: 138 AEISSASHRGFLTSLPELCISLGILLGYVSNYCFGKLTLKLGWRLMLGIAAFPSLILAFG 197

Query: 207 VLTMPESPRWLVMKGKSEEAKKVLIKISTNEIEAEERLKSITEAVAA------AAPGG-- 266
           +  MPESPRWLVM+G+ EEAKK+++ +S  E EAEER + I  A            GG  
Sbjct: 198 ITRMPESPRWLVMQGRLEEAKKIMVLVSNTEEEAEERFRDILTAAEVDVTEIKEVGGGVK 257

Query: 267 -KWNGQGVWKELLIKPTKPIRRILIAAIGINFFMQASGNDAVMYYSPEVFRAAGIQRKRH 326
            K +G+ VW+EL+IKP   +R ILIAA+GI+FF  A+G +AV+ YSP +F+ AG+  K  
Sbjct: 258 KKNHGKSVWRELVIKPRPAVRLILIAAVGIHFFEHATGIEAVVLYSPRIFKKAGVVSKDK 317

Query: 327 LFSVNVVMGITKTCFVLFSALYLDRLGRRPLLLVGSAGMTVALGLLALGSKAMEMGKGRP 386
           L    V +G+TK  F++ +   LD++GRR LLL  + GM  AL  LA+ S  M    GR 
Sbjct: 318 LLLATVGVGLTKAFFIIIATFLLDKVGRRKLLLTSTGGMVFALTSLAV-SLTMVQRFGRL 377

Query: 387 TWAVVLSIVALCGDVALFSIGLGPIAWVYSSEIFPNRMRAQGSSLAVSVNRLVSGVVSMT 446
            WA+ LSIV+    VA FSIGLGPI WVYSSEIFP R+RAQG+S+ V+VNR+++  VSM+
Sbjct: 378 AWALSLSIVSTYAFVAFFSIGLGPITWVYSSEIFPLRLRAQGASIGVAVNRIMNATVSMS 437

Query: 447 FLSISKEITFGGMFMVLSGIMALGTLFFYFFLPETKGKSLEEMEVLF 485
           FLS++K IT GG+F V +GI      FF+F LPETKG  LEEME LF
Sbjct: 438 FLSMTKAITTGGVFFVFAGIAVAAWWFFFFMLPETKGLPLEEMEKLF 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6589914.17.5e-272100.00putative polyol transporter 6, partial [Cucurbita argyrosperma subsp. sororia] >... [more]
XP_022987698.11.8e-27099.20probable polyol transporter 6 [Cucurbita maxima][more]
XP_022960720.19.2e-27099.20probable polyol transporter 6 [Cucurbita moschata][more]
XP_023515881.11.2e-26999.00probable polyol transporter 6 [Cucurbita pepo subsp. pepo][more]
XP_038878586.15.8e-23287.37probable polyol transporter 6 [Benincasa hispida] >XP_038878590.1 probable polyo... [more]
Match NameE-valueIdentityDescription
Q8GXR28.1e-13653.07Probable polyol transporter 6 OS=Arabidopsis thaliana OX=3702 GN=PLT6 PE=2 SV=2[more]
Q8VZ803.4e-13453.65Polyol transporter 5 OS=Arabidopsis thaliana OX=3702 GN=PLT5 PE=1 SV=2[more]
Q9XIH73.0e-13051.80Putative polyol transporter 1 OS=Arabidopsis thaliana OX=3702 GN=PLT1 PE=3 SV=1[more]
Q9XIH61.5e-12950.51Putative polyol transporter 2 OS=Arabidopsis thaliana OX=3702 GN=PLT2 PE=3 SV=1[more]
Q9ZNS01.3e-12853.10Probable polyol transporter 3 OS=Arabidopsis thaliana OX=3702 GN=PLT3 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JK738.9e-27199.20probable polyol transporter 6 OS=Cucurbita maxima OX=3661 GN=LOC111485176 PE=3 S... [more]
A0A6J1H9W54.4e-27099.20probable polyol transporter 6 OS=Cucurbita moschata OX=3662 GN=LOC111461437 PE=3... [more]
A0A6J1CTF71.4e-23185.31probable polyol transporter 6 OS=Momordica charantia OX=3673 GN=LOC111014424 PE=... [more]
A0A5A7TX612.9e-22984.26Putative polyol transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CKQ32.9e-22984.26probable polyol transporter 6 OS=Cucumis melo OX=3656 GN=LOC103501559 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G36670.15.7e-13753.07Major facilitator superfamily protein [more]
AT3G18830.12.4e-13553.65polyol/monosaccharide transporter 5 [more]
AT2G16120.12.1e-13151.80polyol/monosaccharide transporter 1 [more]
AT2G16130.11.1e-13050.51polyol/monosaccharide transporter 2 [more]
AT2G18480.18.9e-13053.10Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003663Sugar/inositol transporterPRINTSPR00171SUGRTRNSPORTcoord: 125..144
score: 37.67
coord: 385..406
score: 34.32
coord: 408..420
score: 38.14
coord: 42..52
score: 44.77
coord: 291..301
score: 37.65
IPR003663Sugar/inositol transporterTIGRFAMTIGR00879TIGR00879coord: 6..480
e-value: 1.2E-106
score: 355.3
IPR005828Major facilitator, sugar transporter-likePFAMPF00083Sugar_trcoord: 35..484
e-value: 7.0E-110
score: 368.0
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 26..488
e-value: 2.7E-129
score: 434.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 29..481
NoneNo IPR availablePANTHERPTHR23500SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTERcoord: 20..492
NoneNo IPR availablePANTHERPTHR23500:SF429POLYOL/MONOSACCHARIDE TRANSPORTER 1coord: 20..492
IPR005829Sugar transporter, conserved sitePROSITEPS00217SUGAR_TRANSPORT_2coord: 130..155
IPR005829Sugar transporter, conserved sitePROSITEPS00216SUGAR_TRANSPORT_1coord: 336..353
IPR005829Sugar transporter, conserved sitePROSITEPS00216SUGAR_TRANSPORT_1coord: 88..105
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 34..473
score: 52.935356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09853-RACarg09853-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity