Carg09431 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09431
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description5'-phosphoribosylglycinamide transformylase
LocationCarg_Chr17: 6067380 .. 6069338 (-)
RNA-Seq ExpressionCarg09431
SyntenyCarg09431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTCAATGTTCTCTCTCTCGGTTTTGCCCAAATCTGTCCATTCCGTTGGTTCGATCTCCTAAACATCCGATCCTTTTGAAGTTTCATTCCTATTCTTCTCGTTCGTCCTTTGTCCGAACCAATCATTCCATTTCGAATAGTGTTTGGTTCAAGGAGAATATTGCATGTAGAAAGAACTGTGAAATCGTTGCGAGTGCCAGTCTCGAAGGGGATGTTTCAAGAACTGTGATTAGGAAGAAGAAACTCGCTGTTTTTGTCTCCGGTGGAGGTTCCAACTTTCGTTCAATACACGAGGCGTCTGTAGAAGGATCAATTCATGGAGAAATTGGTGTTGTGGTTACGAACAAAAGTGGTTGTGGTGGTGCAGAATATGCAAGAAGTAAGAACATTCCAGTAATTTTGTTTCCCAAAACAAAAGATGAACCCCATGGCTTATCTGCTTCTGATCTTGTAGCTACCCTTAGAAGTTTTGAGATAGATTTCATTCTTCTTGCTGGATACTTAAAACTTATACCCCTGGAATTGATCCAAGCCTATTCCAAATATATAATAAACATACACCCATCACTTCTTCCTGCTTTTGGAGGCAAAGGGTATTATGGAATGAAGGTGCACAAGGCAGTGATTGCCTCCGGAGCTCGATTTTCCGGTCCGACGATTCATTTAGTCGATGAGCACTTTGACACTGGAAAGATTCTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATGCAGCAGAGGATCTGGCAGCTAGAGTTCTGAAGGAGGAGCATCGTCTTTATGTCGAGGTAGTAGCAGCGTTATGCGACGATCGGATCGTTTGGAGAGAAGATGGTGTCCCTCTCATTCAAAGCAAAGACAAACCTGGTGAGTTTTACTAGTGAACTGAAAAATGCTTGAAATTTGAGGCTCGGGTGTTTAGTAGTTCAAATGAGATCCATGTTTCTCAGATTCTCTGTAATAATAATATCTGCAAACCAAGAAGTTTGAGCTTCTTCTGCATAAAATAAAGTAGCAGCCTGCCCTGTCTTGGAAGGGTTCATTGAAACAGTTGAGGAATCATTTGACTATGTATAACACAGTTCTTAGTTTTGTGTCATCTGTAAGCTGTACCCAGGTCTGAGCTGATTTTTGAACTCTGTGGGCTTTCATAAGAATGCTTACTAATTGTTCCCCACATTTGTCTTCAAAGGCATCATCTTGTCATTTAAGAGGTGGGCTTGTTGTCTTCATTTGTTTTCAAATGCATTATCTTCTCTTCTTTTCTCTTCTTAGTTCTGAGAACCTTACAATCTATAGGTTGTACAATCGTGTTCTTGTGTTCGTAGTTCGTTCTGTAGATTGTATAATCGTGTTCTTATGTTCTCAATTCGTTCTATAGATTGTACAATCGTGTTCTTGTGTTCCTAGTTCTTATAATTGTGTTCTTGTGTTCTTAGATAGGATCTGTTTTATAGATTGTACAATCGTGTTCTTGTGTTCTTAGTTCTTATAATCTTGTTCTTGTGTTTTTAGTTCTTATGATCGTGTTCTTTGTATTCTTAGTTCTTATAATCGTGTTCTTGTGTTCTTAGTTTTGAGGAGATCGACTCTAGTTTCCATTTGTTTTTTAAACCGTTAACTTCTTTGTTCGATCCAGATTCTTATCTTTCAACTTCTTATATTGTATTCAAAAGCATAGAATATACAACTTTTTAATGCTTTTGGGATGGAGTAGCAACTCCCCTTGAAAAGGCAATCGAGGGCTCGTGAGTTTTACTCGTTAAAGACTAATGTTTTAAACGTGTTATTACTCGTACATCAAATTACAAATACTAGTTATCATTTTTGTCGTTGAAAGAACAGAAAACTCATCAAATATCGACCATAAGAGCGCAGAGAGTCATGAAATGAATAAAGTTTCCTTTGCTAGTGTTAGAGTTTCCTTTGCTAGTGTTAGAGTCATGAAATGA

mRNA sequence

ATGGAAGCTCAATGTTCTCTCTCTCGGTTTTGCCCAAATCTGTCCATTCCGTTGGTTCGATCTCCTAAACATCCGATCCTTTTGAAGTTTCATTCCTATTCTTCTCGTTCGTCCTTTGTCCGAACCAATCATTCCATTTCGAATAGTGTTTGGTTCAAGGAGAATATTGCATGTAGAAAGAACTGTGAAATCGTTGCGAGTGCCAGTCTCGAAGGGGATGTTTCAAGAACTGTGATTAGGAAGAAGAAACTCGCTGTTTTTGTCTCCGGTGGAGGTTCCAACTTTCGTTCAATACACGAGGCGTCTGTAGAAGGATCAATTCATGGAGAAATTGGTGTTGTGGTTACGAACAAAAGTGGTTGTGGTGGTGCAGAATATGCAAGAAGTAAGAACATTCCAGTAATTTTGTTTCCCAAAACAAAAGATGAACCCCATGGCTTATCTGCTTCTGATCTTGTAGCTACCCTTAGAAGTTTTGAGATAGATTTCATTCTTCTTGCTGGATACTTAAAACTTATACCCCTGGAATTGATCCAAGCCTATTCCAAATATATAATAAACATACACCCATCACTTCTTCCTGCTTTTGGAGGCAAAGGGTATTATGGAATGAAGGTGCACAAGGCAGTGATTGCCTCCGGAGCTCGATTTTCCGGTCCGACGATTCATTTAGTCGATGAGCACTTTGACACTGGAAAGATTCTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATGCAGCAGAGGATCTGGCAGCTAGAGTTCTGAAGGAGGAGCATCGTCTTTATGTCGAGGTAGTAGCAGCGTTATGCGACGATCGGATCGTTTGGAGAGAAGATGGTGTCCCTCTCATTCAAAGCAAAGACAAACCTGAAAACTCATCAAATATCGACCATAAGAGCGCAGAGAGTCATGAAATGAATAAAGTTTCCTTTGCTAGTGTTAGAGTTTCCTTTGCTAGTGTTAGAGTCATGAAATGA

Coding sequence (CDS)

ATGGAAGCTCAATGTTCTCTCTCTCGGTTTTGCCCAAATCTGTCCATTCCGTTGGTTCGATCTCCTAAACATCCGATCCTTTTGAAGTTTCATTCCTATTCTTCTCGTTCGTCCTTTGTCCGAACCAATCATTCCATTTCGAATAGTGTTTGGTTCAAGGAGAATATTGCATGTAGAAAGAACTGTGAAATCGTTGCGAGTGCCAGTCTCGAAGGGGATGTTTCAAGAACTGTGATTAGGAAGAAGAAACTCGCTGTTTTTGTCTCCGGTGGAGGTTCCAACTTTCGTTCAATACACGAGGCGTCTGTAGAAGGATCAATTCATGGAGAAATTGGTGTTGTGGTTACGAACAAAAGTGGTTGTGGTGGTGCAGAATATGCAAGAAGTAAGAACATTCCAGTAATTTTGTTTCCCAAAACAAAAGATGAACCCCATGGCTTATCTGCTTCTGATCTTGTAGCTACCCTTAGAAGTTTTGAGATAGATTTCATTCTTCTTGCTGGATACTTAAAACTTATACCCCTGGAATTGATCCAAGCCTATTCCAAATATATAATAAACATACACCCATCACTTCTTCCTGCTTTTGGAGGCAAAGGGTATTATGGAATGAAGGTGCACAAGGCAGTGATTGCCTCCGGAGCTCGATTTTCCGGTCCGACGATTCATTTAGTCGATGAGCACTTTGACACTGGAAAGATTCTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATGCAGCAGAGGATCTGGCAGCTAGAGTTCTGAAGGAGGAGCATCGTCTTTATGTCGAGGTAGTAGCAGCGTTATGCGACGATCGGATCGTTTGGAGAGAAGATGGTGTCCCTCTCATTCAAAGCAAAGACAAACCTGAAAACTCATCAAATATCGACCATAAGAGCGCAGAGAGTCATGAAATGAATAAAGTTTCCTTTGCTAGTGTTAGAGTTTCCTTTGCTAGTGTTAGAGTCATGAAATGA

Protein sequence

MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRKNCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSGCGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPENSSNIDHKSAESHEMNKVSFASVRVSFASVRVMK
Homology
BLAST of Carg09431 vs. NCBI nr
Match: KAG7013988.1 (Phosphoribosylglycinamide formyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638.6 bits (1646), Expect = 2.8e-179
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV
Sbjct: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPENSSNIDHK 300
           PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPENSSNIDHK
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPENSSNIDHK 300

Query: 301 SAESHEMNKVSFASVRVSFASVRVMK 327
           SAESHEMNKVSFASVRVSFASVRVMK
Sbjct: 301 SAESHEMNKVSFASVRVSFASVRVMK 326

BLAST of Carg09431 vs. NCBI nr
Match: XP_022954337.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 560.8 bits (1444), Expect = 7.5e-156
Identity = 285/291 (97.94%), Postives = 287/291 (98.63%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSLSRFCPNLSIPLVRSPKHPILLKF SYSSRSSFVRTNHS S+SVWFKENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFPSYSSRSSFVRTNHSTSSSVWFKENIACRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKS 
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSS 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK IINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDE+FDTGKILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDENFDTGKILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 291

BLAST of Carg09431 vs. NCBI nr
Match: XP_023547957.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.5 bits (1443), Expect = 9.8e-156
Identity = 285/291 (97.94%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSLSRFCPNLSIPLVRSPKHPILLK  SYSSRSSFVRTNHS S+SVWFKE IACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKCPSYSSRSSFVRTNHSTSSSVWFKETIACRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK IINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 291

BLAST of Carg09431 vs. NCBI nr
Match: XP_022992348.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 553.5 bits (1425), Expect = 1.2e-153
Identity = 279/292 (95.55%), Postives = 284/292 (97.26%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSLSRFCPNLSIPLV SPKHPILLKF SYSSR SFVRTNHS SNSVWFKENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVLSPKHPILLKFPSYSSRPSFVRTNHSTSNSVWFKENIACRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           NC++VAS+SLEGDVSR VIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG
Sbjct: 61  NCQVVASSSLEGDVSRIVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK IINIHPSLLP+FGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV
Sbjct: 181 YSKSIINIHPSLLPSFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPE 293
           PVLANDAAEDLAARVLKEEH LYVEVVAALCDDRIVWREDGVPLIQSKD P+
Sbjct: 241 PVLANDAAEDLAARVLKEEHHLYVEVVAALCDDRIVWREDGVPLIQSKDNPD 292

BLAST of Carg09431 vs. NCBI nr
Match: XP_004141701.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis sativus])

HSP 1 Score: 479.9 bits (1234), Expect = 1.7e-131
Identity = 243/291 (83.51%), Postives = 265/291 (91.07%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSL RFCP LSI L+RSP +  LLK    SS SSFV+TNHS   SVWFKENIA RK
Sbjct: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLP--SSSSSFVQTNHSTLRSVWFKENIARRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           N ++V SASL+G+VS+TVI+KKKLAVFVSGGGSNFRSIHEAS+EGS+HGEI VVVTNKSG
Sbjct: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPV+LFPKTKDEP+GLSAS+LVATL+ FEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLKHFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK I+NIHPSLLPAFGGKGYYGMKVHKAV+ SGARFSG TIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           PVLAND+AEDLAARVLKEEH LY EVVAALCD+RI+WR DGVPLIQSK+ P
Sbjct: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKEHP 289

BLAST of Carg09431 vs. ExPASy Swiss-Prot
Match: P52423 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Vigna unguiculata OX=3917 GN=PUR3 PE=2 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 1.1e-101
Identity = 200/311 (64.31%), Postives = 234/311 (75.24%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSI-PLVRSPKHPILLKF---HSYSSRSSFVRTN--------HSIS- 60
           MEAQ  +SRFCP  S+ P +   K P  L F   HS SS       N        H+IS 
Sbjct: 1   MEAQQIISRFCPKSSLAPSIPMVKQPFSLNFPPLHSLSSYPFLQSQNLGFPTGALHAISF 60

Query: 61  ------NSVWFKENIACRKNCEIVASASLEGDV-SRTVIRKKKLAVFVSGGGSNFRSIHE 120
                 +S W    I C K+    A    + +V ++  +R+KKLAVFVSGGGSNFRSIHE
Sbjct: 61  VHKEVCSSSW---RIWCSKSSSSTAEPEEDHEVRAQVTVRRKKLAVFVSGGGSNFRSIHE 120

Query: 121 ASVEGSIHGEIGVVVTNKSGCGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFE 180
           AS +GS+HG++ V+VTNKS CGGA+YAR+  IPVILFPK KDEP GLS  DLV TLR FE
Sbjct: 121 ASKKGSLHGDVTVLVTNKSECGGAQYARNNGIPVILFPKAKDEPKGLSPCDLVDTLRKFE 180

Query: 181 IDFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGP 240
           +DF+LLAGYLKLIP+ELI+A+ + I NIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGP
Sbjct: 181 VDFVLLAGYLKLIPVELIRAFERSIFNIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGP 240

Query: 241 TIHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWRED 292
           TIH VDEH+DTG+ILAQRVVPVLAND AE+LAARVL EEH+LYVEVV ALC++RIVWR+D
Sbjct: 241 TIHFVDEHYDTGRILAQRVVPVLANDTAEELAARVLNEEHQLYVEVVEALCEERIVWRKD 300

BLAST of Carg09431 vs. ExPASy Swiss-Prot
Match: P52422 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PUR3 PE=2 SV=2)

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-81
Identity = 149/223 (66.82%), Postives = 182/223 (81.61%), Query Frame = 0

Query: 71  EGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSGCGGAEYARSK 130
           E D S    R+KKLAVFVSGGGSNFR IHE   +GS++G++ ++VTNK  CGGAEYARS 
Sbjct: 67  EVDGSSHEPRRKKLAVFVSGGGSNFRKIHEGCSDGSVNGDVVLLVTNKKDCGGAEYARSN 126

Query: 131 NIPVILFPKTKDEP-HGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQAYSKYIINIH 190
            IPV++FPK K EP  GLS S+LV  LR + +DF+LLAGYLKLIP+EL+QA+ K I+NIH
Sbjct: 127 GIPVLVFPKAKREPSDGLSPSELVDVLRKYGVDFVLLAGYLKLIPVELVQAFPKRILNIH 186

Query: 191 PSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVVPVLANDAAE 250
           P+LLPAFGGKG YG+KVHKAV+ SGAR+SGPTIH V+E +DTG+ILAQ  V V+AND  E
Sbjct: 187 PALLPAFGGKGLYGIKVHKAVLESGARYSGPTIHFVNEEYDTGRILAQSAVRVIANDTPE 246

Query: 251 DLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPE 293
           +LA RVL EEH+LYVEVV A+C++RI WREDGVPLIQ+K  P+
Sbjct: 247 ELAKRVLHEEHKLYVEVVGAICEERIKWREDGVPLIQNKQNPD 289

BLAST of Carg09431 vs. ExPASy Swiss-Prot
Match: Q42805 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Glycine max OX=3847 GN=PUR3 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 160/292 (54.79%), Postives = 199/292 (68.15%), Query Frame = 0

Query: 15  SIPLVR---SPKHPILLKFHSY-SSRSSFVRTNHSISNSVWFKENIACRKNCEIVASASL 74
           SIP+V+   SPK P L     Y SS+S  +       + +       C  +C+ +  +S 
Sbjct: 5   SIPIVKQQFSPKFPRLPSSSLYPSSQSQNLNVPSGAFHPISIVHKEVCSSSCKRIWCSSS 64

Query: 75  EGDV----------SRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 134
                         ++  +R+KKL VFVSGGG+NFR+IHEA+  GS+HG++ V+VTNKS 
Sbjct: 65  SSSTAEPKEGHEVRAQVTVRRKKLGVFVSGGGTNFRAIHEATKRGSLHGDVLVLVTNKSD 124

Query: 135 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 194
           CGGAEYAR+  IPVIL+  +KDE +G   SDLV TLR FE+DFILLAGYL L        
Sbjct: 125 CGGAEYARNNGIPVILYHISKDESNG---SDLVDTLRKFEVDFILLAGYLNLYQWNDPSL 184

Query: 195 YSKYIINIHPSLL-PAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRV 254
              YI   H S+   +F  +G +GMKVHKAVIASGARFSGPTIH VDEH+DTG+ILAQRV
Sbjct: 185 QKIYI--QHSSITSSSFWRQGIHGMKVHKAVIASGARFSGPTIHFVDEHYDTGRILAQRV 244

Query: 255 VPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           VPV AND  E+LAARVLKEEH+LYVEVV ALC++R+VWR+DGVPLIQSK+ P
Sbjct: 245 VPVQANDTVEELAARVLKEEHQLYVEVVEALCEERVVWRQDGVPLIQSKENP 291

BLAST of Carg09431 vs. ExPASy Swiss-Prot
Match: P00967 (Trifunctional purine biosynthetic protein adenosine-3 OS=Drosophila melanogaster OX=7227 GN=Gart PE=1 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 9.9e-26
Identity = 63/193 (32.64%), Postives = 110/193 (56.99%), Query Frame = 0

Query: 80   RKKKLAVFVSGGGSNFRSIHEASVEGS--IHGEIGVVVTNKSGCGGAEYARSKNIPVILF 139
            R+K++AV +SG GSN +++ +A+ + +  IH ++ +V++NK G  G + A    IP ++ 
Sbjct: 1152 RRKRVAVLISGTGSNLQALIDATRDSAQGIHADVVLVISNKPGVLGLQRATQAGIPSLVI 1211

Query: 140  PKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFG 199
                     +  ++L   L++  +D I LAG+++++    ++ +   ++NIHPSLLP   
Sbjct: 1212 SHKDFASREVYDAELTRNLKAARVDLICLAGFMRVLSAPFVREWRGRLVNIHPSLLPK-- 1271

Query: 200  GKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLK 259
               Y G+ V K  + +G + SG T+H VDE  DTG I+ Q  VP+L +D  + L  R+ K
Sbjct: 1272 ---YPGLHVQKQALEAGEKESGCTVHFVDEGVDTGAIIVQAAVPILPDDDEDSLTQRIHK 1331

Query: 260  EEHRLYVEVVAAL 271
             EH  +   +A L
Sbjct: 1332 AEHWAFPRALAML 1339

BLAST of Carg09431 vs. ExPASy Swiss-Prot
Match: P43846 (Phosphoribosylglycinamide formyltransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=purN PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.2e-25
Identity = 69/213 (32.39%), Postives = 119/213 (55.87%), Query Frame = 0

Query: 82  KKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSGCGGAEYARSKNIPVILFPKTK 141
           KK+AV +SG G+N ++I +A   G I  +I  V++NK+   G   A+   IP  +F + K
Sbjct: 2   KKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLR-K 61

Query: 142 DEPHGLSASDLVAT-LRSFEIDFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFGGKG 201
           +  + L   D +   L+S  +D I+LAGY+K++  +  Q ++  I+NIHPSLLP      
Sbjct: 62  NFSNNLEMDDAIGDYLQSLAVDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPK----- 121

Query: 202 YYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLKEEH 261
           Y G+  ++  I +G    G T+H V+E  D G I+ Q  VP+   D+ E++ AR  ++E+
Sbjct: 122 YAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPEDSIEEVEARTREQEY 181

Query: 262 RLYVEVVAALCDDRIVWREDGVPLIQSKDKPEN 294
           ++Y  V+    + R+  + D +  +  K  P++
Sbjct: 182 QIYPLVIKWFTEGRLRLK-DNLAYLDGKALPKS 207

BLAST of Carg09431 vs. ExPASy TrEMBL
Match: A0A6J1GQR1 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456613 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.6e-156
Identity = 285/291 (97.94%), Postives = 287/291 (98.63%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSLSRFCPNLSIPLVRSPKHPILLKF SYSSRSSFVRTNHS S+SVWFKENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFPSYSSRSSFVRTNHSTSSSVWFKENIACRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKS 
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSS 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK IINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDE+FDTGKILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDENFDTGKILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 291

BLAST of Carg09431 vs. ExPASy TrEMBL
Match: A0A6J1JYY4 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488685 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.8e-154
Identity = 279/292 (95.55%), Postives = 284/292 (97.26%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSLSRFCPNLSIPLV SPKHPILLKF SYSSR SFVRTNHS SNSVWFKENIACRK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVLSPKHPILLKFPSYSSRPSFVRTNHSTSNSVWFKENIACRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           NC++VAS+SLEGDVSR VIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG
Sbjct: 61  NCQVVASSSLEGDVSRIVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK IINIHPSLLP+FGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV
Sbjct: 181 YSKSIINIHPSLLPSFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPE 293
           PVLANDAAEDLAARVLKEEH LYVEVVAALCDDRIVWREDGVPLIQSKD P+
Sbjct: 241 PVLANDAAEDLAARVLKEEHHLYVEVVAALCDDRIVWREDGVPLIQSKDNPD 292

BLAST of Carg09431 vs. ExPASy TrEMBL
Match: A0A0A0K7R9 (Formyl_trans_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450470 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 8.2e-132
Identity = 243/291 (83.51%), Postives = 265/291 (91.07%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCSL RFCP LSI L+RSP +  LLK    SS SSFV+TNHS   SVWFKENIA RK
Sbjct: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLP--SSSSSFVQTNHSTLRSVWFKENIARRK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           N ++V SASL+G+VS+TVI+KKKLAVFVSGGGSNFRSIHEAS+EGS+HGEI VVVTNKSG
Sbjct: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPV+LFPKTKDEP+GLSAS+LVATL+ FEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLKHFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK I+NIHPSLLPAFGGKGYYGMKVHKAV+ SGARFSG TIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           PVLAND+AEDLAARVLKEEH LY EVVAALCD+RI+WR DGVPLIQSK+ P
Sbjct: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKEHP 289

BLAST of Carg09431 vs. ExPASy TrEMBL
Match: A0A1S3CGP8 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500692 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.4e-131
Identity = 244/294 (82.99%), Postives = 263/294 (89.46%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHS---YSSRSSFVRTNHSISNSVWFKENIA 60
           MEAQCSL R CPN S PL+RSP +  LLK  S   +SS SSFV+TNHS   SVWFKENIA
Sbjct: 1   MEAQCSLCRSCPNFSFPLIRSPNYSFLLKLPSSSFFSSSSSFVQTNHSTLRSVWFKENIA 60

Query: 61  CRKNCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTN 120
           CRK    V SASLEG+VS+TVI+KKKLAVFVSGGGSNFRSIHEAS+EG +HGEI VVVTN
Sbjct: 61  CRK----VVSASLEGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGLVHGEIVVVVTN 120

Query: 121 KSGCGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLEL 180
           KS CGGAEYARSKNIPV+LFPKTKDEPHGLSAS+LVATLR FEIDFILLAGYLKLIPLEL
Sbjct: 121 KSSCGGAEYARSKNIPVLLFPKTKDEPHGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180

Query: 181 IQAYSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQ 240
           IQAYSK I+NIHPSLLPAFGGKGYYGMKVHKAV+ SGARFSG TIHLVDEHFDTG+ILAQ
Sbjct: 181 IQAYSKCILNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240

Query: 241 RVVPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           RVVPVL+ND+AEDLAARVLKEEHRLY EVVAALCD+RI+WR DGVPLIQSK+ P
Sbjct: 241 RVVPVLSNDSAEDLAARVLKEEHRLYAEVVAALCDERIIWRSDGVPLIQSKENP 290

BLAST of Carg09431 vs. ExPASy TrEMBL
Match: A0A6J1DNE2 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022206 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 3.1e-123
Identity = 232/291 (79.73%), Postives = 254/291 (87.29%), Query Frame = 0

Query: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFHSYSSRSSFVRTNHSISNSVWFKENIACRK 60
           MEAQCS SRFCPNLS+   RSPK   LLK  S SS  SF R++   S SVW K+N+  +K
Sbjct: 1   MEAQCSFSRFCPNLSVSFFRSPKLSFLLKLPSASS-CSFARSSVPTSPSVWLKKNVGVKK 60

Query: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120
           N ++V   SLEG+VSRTVIR+KKLAVFVSGGGSNFRSIHEAS+EGSI+G+I V+VTNK  
Sbjct: 61  NSQMVVRVSLEGEVSRTVIRRKKLAVFVSGGGSNFRSIHEASLEGSINGDIVVLVTNKID 120

Query: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPVILFPKTKDEP  LS SDLVA LR  EIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPDRLSPSDLVANLRRLEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240
           YSK I+NIHPSLLPAFGGKGYYGMKVHK+VIASGAR+SGPTIH VDEHFDTG+ILAQRVV
Sbjct: 181 YSKSILNIHPSLLPAFGGKGYYGMKVHKSVIASGARYSGPTIHFVDEHFDTGRILAQRVV 240

Query: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKP 292
           PVLANDAA+DLAARVLKEEH LYVEVVAALC++RIVWREDGVPLIQSK+ P
Sbjct: 241 PVLANDAAQDLAARVLKEEHCLYVEVVAALCEERIVWREDGVPLIQSKENP 290

BLAST of Carg09431 vs. TAIR 10
Match: AT1G31220.1 (Formyl transferase )

HSP 1 Score: 304.7 bits (779), Expect = 9.0e-83
Identity = 149/223 (66.82%), Postives = 182/223 (81.61%), Query Frame = 0

Query: 71  EGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSGCGGAEYARSK 130
           E D S    R+KKLAVFVSGGGSNFR IHE   +GS++G++ ++VTNK  CGGAEYARS 
Sbjct: 67  EVDGSSHEPRRKKLAVFVSGGGSNFRKIHEGCSDGSVNGDVVLLVTNKKDCGGAEYARSN 126

Query: 131 NIPVILFPKTKDEP-HGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQAYSKYIINIH 190
            IPV++FPK K EP  GLS S+LV  LR + +DF+LLAGYLKLIP+EL+QA+ K I+NIH
Sbjct: 127 GIPVLVFPKAKREPSDGLSPSELVDVLRKYGVDFVLLAGYLKLIPVELVQAFPKRILNIH 186

Query: 191 PSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVVPVLANDAAE 250
           P+LLPAFGGKG YG+KVHKAV+ SGAR+SGPTIH V+E +DTG+ILAQ  V V+AND  E
Sbjct: 187 PALLPAFGGKGLYGIKVHKAVLESGARYSGPTIHFVNEEYDTGRILAQSAVRVIANDTPE 246

Query: 251 DLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPE 293
           +LA RVL EEH+LYVEVV A+C++RI WREDGVPLIQ+K  P+
Sbjct: 247 ELAKRVLHEEHKLYVEVVGAICEERIKWREDGVPLIQNKQNPD 289

BLAST of Carg09431 vs. TAIR 10
Match: AT5G47435.2 (formyltetrahydrofolate deformylase, putative )

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-09
Identity = 46/176 (26.14%), Postives = 75/176 (42.61%), Query Frame = 0

Query: 104 EGSIHGEIGVVVTNKSGCGGAEYAR---SKNIPVILFPKTKDEPHGLSASDLVATLRSFE 163
           +G +  +I  V++N          R      IP      TK+        +LV      +
Sbjct: 148 DGKLPVDITCVISNHERASNTHVMRFLERHGIPYHYVSTTKENKREDDILELVK-----D 207

Query: 164 IDFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGP 223
            DF++LA Y++++    ++ Y K +INIH  LLP+F G GY      K    +G +  G 
Sbjct: 208 TDFLVLARYMQILSGNFLKGYGKDVINIHHGLLPSFKG-GYPA----KQAFDAGVKLIGA 267

Query: 224 TIHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIV 277
           T H V E  D+G I+ Q V  V   D       +    E +     + + C+ R++
Sbjct: 268 TSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQKSEDLEKKCLTRAIKSYCELRVL 313

BLAST of Carg09431 vs. TAIR 10
Match: AT5G47435.1 (formyltetrahydrofolate deformylase, putative )

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-09
Identity = 46/176 (26.14%), Postives = 75/176 (42.61%), Query Frame = 0

Query: 104 EGSIHGEIGVVVTNKSGCGGAEYAR---SKNIPVILFPKTKDEPHGLSASDLVATLRSFE 163
           +G +  +I  V++N          R      IP      TK+        +LV      +
Sbjct: 148 DGKLPVDITCVISNHERASNTHVMRFLERHGIPYHYVSTTKENKREDDILELVK-----D 207

Query: 164 IDFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGP 223
            DF++LA Y++++    ++ Y K +INIH  LLP+F G GY      K    +G +  G 
Sbjct: 208 TDFLVLARYMQILSGNFLKGYGKDVINIHHGLLPSFKG-GYPA----KQAFDAGVKLIGA 267

Query: 224 TIHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIV 277
           T H V E  D+G I+ Q V  V   D       +    E +     + + C+ R++
Sbjct: 268 TSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQKSEDLEKKCLTRAIKSYCELRVL 313

BLAST of Carg09431 vs. TAIR 10
Match: AT4G17360.1 (Formyl transferase )

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-09
Identity = 34/115 (29.57%), Postives = 57/115 (49.57%), Query Frame = 0

Query: 162 DFILLAGYLKLIPLELIQAYSKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPT 221
           DF++LA Y++L+    ++ Y K +INIH  LLP+F G+        K    +G +  G T
Sbjct: 209 DFLVLARYMQLLSGNFLKGYGKDVINIHHGLLPSFKGRNPV-----KQAFDAGVKLIGAT 268

Query: 222 IHLVDEHFDTGKILAQRVVPVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIV 277
            H V E  D+G I+ Q V  V   D       +    E +  ++ + + C+ R++
Sbjct: 269 THFVTEELDSGPIIEQMVERVSHRDNLRSFVQKSEDLEKKCLMKAIKSYCELRVL 318

BLAST of Carg09431 vs. TAIR 10
Match: AT1G66520.1 (formyltransferase, putative )

HSP 1 Score: 43.9 bits (102), Expect = 2.9e-04
Identity = 36/151 (23.84%), Postives = 65/151 (43.05%), Query Frame = 0

Query: 124 AEYARSKNIP--VILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQAY 183
           A+YA  K +P  +I  P+   +   LSA      LR  + +  + A Y  ++P + ++  
Sbjct: 79  AQYALDKGLPSDLIFSPEKAGDEAFLSA------LRELQPELCITAAYGNILPTKFLKIP 138

Query: 184 SKYIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVVP 243
               +NIHPSLLP      Y G    +  +  G   +G ++       D G ++A +   
Sbjct: 139 VHGTVNIHPSLLPL-----YRGAAPVQRALQDGVPETGVSLAFTVRKLDAGPVIASKRFQ 198

Query: 244 VLANDAAEDLAARVLKEEHRLYVEVVAALCD 273
           V     A +L + +  E   L +  + ++ D
Sbjct: 199 VDDLIKAPELLSFLFSEGSNLLIRELPSIFD 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013988.12.8e-179100.00Phosphoribosylglycinamide formyltransferase, chloroplastic, partial [Cucurbita a... [more]
XP_022954337.17.5e-15697.94phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita moschata][more]
XP_023547957.19.8e-15697.94phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita pepo subsp... [more]
XP_022992348.11.2e-15395.55phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita maxima][more]
XP_004141701.11.7e-13183.51phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P524231.1e-10164.31Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Vigna unguiculata ... [more]
P524221.3e-8166.82Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Arabidopsis thalia... [more]
Q428051.2e-7454.79Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Glycine max OX=384... [more]
P009679.9e-2632.64Trifunctional purine biosynthetic protein adenosine-3 OS=Drosophila melanogaster... [more]
P438462.2e-2532.39Phosphoribosylglycinamide formyltransferase OS=Haemophilus influenzae (strain AT... [more]
Match NameE-valueIdentityDescription
A0A6J1GQR13.6e-15697.94phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1JYY45.8e-15495.55phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita maxima O... [more]
A0A0A0K7R98.2e-13283.51Formyl_trans_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450... [more]
A0A1S3CGP81.4e-13182.99phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucumis melo OX=36... [more]
A0A6J1DNE23.1e-12379.73phosphoribosylglycinamide formyltransferase, chloroplastic OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
AT1G31220.19.0e-8366.82Formyl transferase [more]
AT5G47435.21.0e-0926.14formyltetrahydrofolate deformylase, putative [more]
AT5G47435.11.0e-0926.14formyltetrahydrofolate deformylase, putative [more]
AT4G17360.12.3e-0929.57Formyl transferase [more]
AT1G66520.12.9e-0423.84formyltransferase, putative [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002376Formyl transferase, N-terminalPFAMPF00551Formyl_trans_Ncoord: 82..267
e-value: 1.3E-53
score: 181.4
IPR004607Phosphoribosylglycinamide formyltransferaseTIGRFAMTIGR00639TIGR00639coord: 82..275
e-value: 2.0E-48
score: 162.4
IPR004607Phosphoribosylglycinamide formyltransferaseHAMAPMF_01930PurNcoord: 83..273
score: 26.694416
IPR004607Phosphoribosylglycinamide formyltransferaseCDDcd08645FMT_core_GARTcoord: 83..270
e-value: 2.24567E-83
score: 248.071
NoneNo IPR availableGENE3D3.40.50.170coord: 68..293
e-value: 1.9E-64
score: 218.8
NoneNo IPR availablePANTHERPTHR43369PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASEcoord: 66..288
IPR036477Formyl transferase, N-terminal domain superfamilySUPERFAMILY53328Formyltransferasecoord: 82..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09431-RACarg09431-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006189 'de novo' IMP biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004644 phosphoribosylglycinamide formyltransferase activity
molecular_function GO:0016742 hydroxymethyl-, formyl- and related transferase activity