Carg08897 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08897
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAvrRpt-cleavage domain-containing protein
LocationCarg_Chr02: 9360738 .. 9363411 (-)
RNA-Seq ExpressionCarg08897
SyntenyCarg08897
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCTGCTTTATTATTAACAATGGAGGTTCCTTCATCACGAAGCCAATTCTCTCTCTCTTTCTCTCTCTAGATTCATCGCTTCCGTATTCTCCTCTTCTTCCTCGGATTCCGGCGATCCTCTTCGCCGTGATTTCTCGTCGGAACTCGAGTCTGCTCGCAGTCCGGCAGTTTTTTCCCATTTCGAAGTAGTTCCCGCCGCCGCGCACGTCTGACTCCGGTGGATAAACCACCATGGCTGTAAGCTTCCATTTCTCTTCTTTTGTTTATTTGAATGTTTCAGTGTGCGTTTTCTTTGTTATGTTCTGTTTGGCTGCTTGGAAAATATCCTGGTTAGTGTGAAAACAGAGAATGAGAATTCAAAATTTGCTTTCCGACTGGAACTAAACGAGTTGCGTTTTCTCATTTCATTTGCCCTAAAATGATGTGAGAGATCTAAATCAAATTTCAGGCTCTGTTGAATGGGGATGGAAATCGAGAAACGGTTATTGGAACCGAATAGTTTTTTAGGATTGAATCAATTTGGAGAACTGTTCAAAAGCTTTCTAGATGTTATTTAGTCTGAGCGATTTAAAAATGGTTTTCACTTTCATCGTTTTCATACGGACGAAATTATTGCTAGTTTTCAATGCTTTTTGCTTCTGTTTGTTCATATGCTAGTGGTGGGTGCCACTTTTTCTTGTAGAAAGGTTCAATCACGAGCATCTGATTCACACTCAGAATTAAATTAATACTACTTTTGCCATTCTACTTAATCATTATAAGAAAGATGCATACATCCTTCCATATAAAACAATATCCTTGTACAGATGATAAACAAGTTGAGCGAGCTTATTTAGAATACATGTGGGGCTTTATTTTATTTTATTGAGCTCCCTTTTTGAGTATAGGGACATTCAAGTATTCTCTCAAACAATCATCTGTTCATTAATTCTTTCTAATTCTGCTTTATATTTTGAACTTTATGGAAAAGGTCTAGTCTCTGAATATGTGATTTTTGTCGAATTCTTAACAAATTCCTATAAACCAAGCTCAATTCTCCATCTTCTCAATGGCGTTTAAGTTTCTACATTGCCCATAACTTAGGGAAACACTAAAAAATCTCTGTTTCCTTTTTTGTTTTCATTTCACATTTGTTGTTTTATGATTGGACTCTTTATAATAGCTGTTTTGACATTTTGGTTCAAATTGCTAGATAGCGTAGATCCCATGAAGGCAAAGGAAAATCTGTTTGGATTAACATTATGCATTTCATCTCTAGCCTTAGGCACTAATTATGGATGGAATTCTTTTGAGTGCCCCCCACTTTTTCAAAGAAATGAGCAATTTTCCAAACTTGTGAAGGAATGGAAACCTTCTTGTTTTTGCTGAAATTGTCAAAGTTGCCGTCTGTTCTTTTGGGATCAGTTGGTAAATATGGATATGGATGATATTGATTCTTATTCAAAGACTTATATTTGTGGTGCATCTTTTAGGTCAATTGTAGGTAATGATCATATATGGTTCATACATTAGAGCTATCTCAAGCACGTAGAAGTGAGGATTTAAAATCTTGAACATTTTGGTTGATAGTGTAGGTGAGATCCCACGTTGGTTGGTTGGTTGGTTGGGGAGGGTGAAACATTTTTTTATAAGAGTGTGGAAACCTCTCCCTACTAGATGCGTTCTAGAAACCTTGAGGAAAAATGACAATATGTTCTAGCGGTGGACTTGGGCTATTAGAGCATATGTCTGAACTAGAACTAGAACTAGATGAATTAATGTTCAAATTGACATAGCTATCTTTCTATTTGTTCATAATCGATACGGAACTGAAATGGTTGCAAACTAACCAAAGGACAAAGAAGTTGCGGTTCCAAAATTTGGTGAATGGAACGACAGGGACGCCACACCGCCAGACAACTACACAGCTATTTTCACCAAGGTGCGGGAAGAGAGACAAGACCAGGGAGCAAGGACGCCGACGCTGCCACAGAACAGTAACATCCCAGATAACCAAAATGATGAACAAAATCAGAAGGTAATTGAAGAACTCTCTTCCATTTCTCAGCCAACCACGTGAGATTACTGAGATTTGATAATGCAAATGTCTTACATTCTTCTGTTCTTCCTCAAAATTTTCAGAAATGCTGTTGTTTTCCATGGCCAAGCAAATGATATTGTTCTTTGCATTTTCCACGTGAGATTACTTAGATTGCATGAACATGTGAGAATAATGTAGATTATTTCATGGATCTGGCTGCAGCTCACCTCCTTCAATGCCATCTGATTGAAAGTGTTTGTCTGTGGCCAAAGTTGTAAGCAAAGACAAAATTTGATTGTGTTGTTTGGGGTTTACAATTTTTTCTTATTTCGAGATTCATCTGTTGGATATCTATTCTTCATTTTAATAACTGTACTGTGATTTTGGTGTTATGTGTTCTTATTTAATGTTGTAATGTGTTCTTATTTAATGTTGTAATGTGTTCTTATTTAGTTACTATAATTTTAGAAAATTACAATTGTAAGTGATATTATTTTGTTAAGTATTTCAAAACATTTTTATTTTAAATTGAAGGTTTGTAGTTCTATGACGAAGAAATGCCTATTTGCTCAACATAATAGGACTCGTGTGGATTCGTCCCCAATAGGGCAACAAATAGGGGAAGGAGTAGGAGAAAATCGTCTTTGTTAG

mRNA sequence

ATTTTCTGCTTTATTATTAACAATGGAGGTTCCTTCATCACGAAGCCAATTCTCTCTCTCTTTCTCTCTCTAGATTCATCGCTTCCGTATTCTCCTCTTCTTCCTCGGATTCCGGCGATCCTCTTCGCCGTGATTTCTCGTCGGAACTCGAGTCTGCTCGCAGTCCGGCAGTTTTTTCCCATTTCGAAGTAGTTCCCGCCGCCGCGCACGTCTGACTCCGGTGGATAAACCACCATGGCTGACAAAGAAGTTGCGGTTCCAAAATTTGGTGAATGGAACGACAGGGACGCCACACCGCCAGACAACTACACAGCTATTTTCACCAAGGTGCGGGAAGAGAGACAAGACCAGGGAGCAAGGACGCCGACGCTGCCACAGAACAGTAACATCCCAGATAACCAAAATGATGAACAAAATCAGAAGGTTTGTAGTTCTATGACGAAGAAATGCCTATTTGCTCAACATAATAGGACTCGTGTGGATTCGTCCCCAATAGGGCAACAAATAGGGGAAGGAGTAGGAGAAAATCGTCTTTGTTAG

Coding sequence (CDS)

ATGGCTGACAAAGAAGTTGCGGTTCCAAAATTTGGTGAATGGAACGACAGGGACGCCACACCGCCAGACAACTACACAGCTATTTTCACCAAGGTGCGGGAAGAGAGACAAGACCAGGGAGCAAGGACGCCGACGCTGCCACAGAACAGTAACATCCCAGATAACCAAAATGATGAACAAAATCAGAAGGTTTGTAGTTCTATGACGAAGAAATGCCTATTTGCTCAACATAATAGGACTCGTGTGGATTCGTCCCCAATAGGGCAACAAATAGGGGAAGGAGTAGGAGAAAATCGTCTTTGTTAG

Protein sequence

MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQNQKVCSSMTKKCLFAQHNRTRVDSSPIGQQIGEGVGENRLC
Homology
BLAST of Carg08897 vs. NCBI nr
Match: KAG7036250.1 (hypothetical protein SDJN02_03052 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 211.8 bits (538), Expect = 2.7e-51
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 0

Query: 1   MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60
           MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ
Sbjct: 1   MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60

Query: 61  NQKVCSSMTKKCLFAQHNRTRVDSSPIGQQIGEGVGENRLC 102
           NQKVCSSMTKKCLFAQHNRTRVDSSPIGQQIGEGVGENRLC
Sbjct: 61  NQKVCSSMTKKCLFAQHNRTRVDSSPIGQQIGEGVGENRLC 101

BLAST of Carg08897 vs. NCBI nr
Match: KAG6606311.1 (RPM1-interacting protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 135.6 bits (340), Expect = 2.4e-28
Identity = 64/65 (98.46%), Postives = 64/65 (98.46%), Query Frame = 0

Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60
          MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ
Sbjct: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60

Query: 61 NQKVC 66
          NQK C
Sbjct: 61 NQKKC 65

BLAST of Carg08897 vs. NCBI nr
Match: XP_038888923.1 (RPM1-interacting protein 4-like [Benincasa hispida])

HSP 1 Score: 81.3 bits (199), Expect = 5.4e-12
Identity = 41/66 (62.12%), Postives = 48/66 (72.73%), Query Frame = 0

Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQN-SNIPDNQNDE 60
          MAD E  VPKFGEW+DRDA  P+NYTAIF KVRE RQ+ G R  T P++ +N  +NQN E
Sbjct: 1  MADNEATVPKFGEWDDRDAKAPENYTAIFKKVREGRQEPGIRALTPPRSINNSTNNQNHE 60

Query: 61 QNQKVC 66
           NQK C
Sbjct: 61 HNQKKC 66

BLAST of Carg08897 vs. NCBI nr
Match: XP_022887205.1 (RPM1-interacting protein 4-like [Olea europaea var. sylvestris] >CAA2935327.1 Hypothetical predicted protein [Olea europaea subsp. europaea])

HSP 1 Score: 60.5 bits (145), Expect = 9.9e-06
Identity = 31/65 (47.69%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQN--DEQ 62
           D   AVPKFGEW+D D      Y+ IFTKVREERQ  G R P +P  ++  + Q+    +
Sbjct: 156 DDSPAVPKFGEWDDTDPAAAAGYSGIFTKVREERQSGGGRVPAMPTETSFSNVQSRYGNE 215

Query: 63  NQKVC 66
           NQK C
Sbjct: 216 NQKGC 220

BLAST of Carg08897 vs. NCBI nr
Match: XP_015086553.1 (RPM1-interacting protein 4 isoform X1 [Solanum pennellii])

HSP 1 Score: 58.5 bits (140), Expect = 3.8e-05
Identity = 27/64 (42.19%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 2   ADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQN 61
           +D+  AVP+FGEW++ D    DNYT IF KVREE+Q   + TP+   N+    N  ++Q 
Sbjct: 189 SDRGAAVPRFGEWDENDPQSADNYTHIFNKVREEKQGNPSGTPSRTSNNTQKHNSEEKQR 248

Query: 62  QKVC 66
           +  C
Sbjct: 249 KWCC 252

BLAST of Carg08897 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.5e-04
Identity = 26/58 (44.83%), Postives = 35/58 (60.34%), Query Frame = 0

Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQ-----DQGARTPTLPQNSNIPDN 56
           +K   VPKFG+W++ + +  D YT IF KVREER         +RTPT  Q+S  P+N
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPT-HQSSRNPNN 199

BLAST of Carg08897 vs. ExPASy TrEMBL
Match: A0A0A0L950 (AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G630240 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.9e-11
Identity = 43/65 (66.15%), Postives = 49/65 (75.38%), Query Frame = 0

Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGAR-TPTLPQNSNIPDNQNDE 60
          MAD E AVPKFGEW+D DA  P+NYTAIF KVREERQDQ  R TPT   +S   ++QN E
Sbjct: 1  MADNETAVPKFGEWDDGDAKAPENYTAIFNKVREERQDQTVRGTPTRLIDST--NSQNHE 60

Query: 61 QNQKV 65
          QNQK+
Sbjct: 61 QNQKL 63

BLAST of Carg08897 vs. ExPASy TrEMBL
Match: A0A6N2AQE8 (AvrRpt-cleavage domain-containing protein OS=Solanum chilense OX=4083 GN=EJD97_006924 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.8e-05
Identity = 27/64 (42.19%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 2   ADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQN 61
           +D+  AVP+FGEW++ D    DNYT IF KVREE+Q   + TP+   N+    N  ++Q 
Sbjct: 188 SDRGAAVPRFGEWDENDPQSADNYTHIFNKVREEKQGNPSGTPSRTSNNTQKHNSEEKQR 247

Query: 62  QKVC 66
           +  C
Sbjct: 248 KWCC 251

BLAST of Carg08897 vs. ExPASy TrEMBL
Match: A0A6A6K948 (AvrRpt-cleavage domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_026145 PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.4e-05
Identity = 26/58 (44.83%), Postives = 37/58 (63.79%), Query Frame = 0

Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQND 59
          M DK  AVPKFGEW++ +    D YT IF KVREERQ +  + P +P  S++ + + +
Sbjct: 34 MPDKGAAVPKFGEWDENNPASADGYTHIFNKVREERQIEAGKVPGMPTESSMSNTRKE 91

BLAST of Carg08897 vs. ExPASy TrEMBL
Match: M1CPC9 (RIN4c protein OS=Solanum tuberosum OX=4113 GN=102596147 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.1e-05
Identity = 27/64 (42.19%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 2   ADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQN 61
           +D+  AVP+FGEW++ D    DNYT IF KVREE+Q   + TP+   N+    N  ++Q 
Sbjct: 190 SDRGAAVPRFGEWDENDPQSADNYTHIFNKVREEKQGNPSGTPSRASNNTQKHNSEEKQM 249

Query: 62  QKVC 66
           +  C
Sbjct: 250 KWCC 253

BLAST of Carg08897 vs. ExPASy TrEMBL
Match: M1CPD0 (RIN4c protein OS=Solanum tuberosum OX=4113 GN=102596147 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.1e-05
Identity = 27/64 (42.19%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 2   ADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQN 61
           +D+  AVP+FGEW++ D    DNYT IF KVREE+Q   + TP+   N+    N  ++Q 
Sbjct: 189 SDRGAAVPRFGEWDENDPQSADNYTHIFNKVREEKQGNPSGTPSRASNNTQKHNSEEKQM 248

Query: 62  QKVC 66
           +  C
Sbjct: 249 KWCC 252

BLAST of Carg08897 vs. TAIR 10
Match: AT3G25070.1 (RPM1 interacting protein 4 )

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-05
Identity = 26/58 (44.83%), Postives = 35/58 (60.34%), Query Frame = 0

Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQ-----DQGARTPTLPQNSNIPDN 56
           +K   VPKFG+W++ + +  D YT IF KVREER         +RTPT  Q+S  P+N
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPT-HQSSRNPNN 199

BLAST of Carg08897 vs. TAIR 10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.6e-04
Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = 0

Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQG 41
          MADK   +PKFGEW+  D +  + +T IF K R E++  G
Sbjct: 1  MADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGG 40

BLAST of Carg08897 vs. TAIR 10
Match: AT5G55850.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 41.6 bits (96), Expect = 4.4e-04
Identity = 17/40 (42.50%), Postives = 25/40 (62.50%), Query Frame = 0

Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQG 41
          M+DK   +PKFGEW+  D    + +T IF K R+E++  G
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGG 40

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036250.12.7e-51100.00hypothetical protein SDJN02_03052 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6606311.12.4e-2898.46RPM1-interacting protein 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038888923.15.4e-1262.12RPM1-interacting protein 4-like [Benincasa hispida][more]
XP_022887205.19.9e-0647.69RPM1-interacting protein 4-like [Olea europaea var. sylvestris] >CAA2935327.1 Hy... [more]
XP_015086553.13.8e-0542.19RPM1-interacting protein 4 isoform X1 [Solanum pennellii][more]
Match NameE-valueIdentityDescription
Q8GYN51.5e-0444.83RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9502.9e-1166.15AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G63... [more]
A0A6N2AQE81.8e-0542.19AvrRpt-cleavage domain-containing protein OS=Solanum chilense OX=4083 GN=EJD97_0... [more]
A0A6A6K9482.4e-0544.83AvrRpt-cleavage domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714... [more]
M1CPC93.1e-0542.19RIN4c protein OS=Solanum tuberosum OX=4113 GN=102596147 PE=4 SV=1[more]
M1CPD03.1e-0542.19RIN4c protein OS=Solanum tuberosum OX=4113 GN=102596147 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G25070.11.1e-0544.83RPM1 interacting protein 4 [more]
AT2G04410.12.6e-0445.00RPM1-interacting protein 4 (RIN4) family protein [more]
AT5G55850.14.4e-0442.50RPM1-interacting protein 4 (RIN4) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008700RIN4, pathogenic type III effector avirulence factor Avr cleavage sitePFAMPF05627AvrRpt-cleavagecoord: 3..37
e-value: 3.8E-13
score: 48.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..64
NoneNo IPR availablePANTHERPTHR33159:SF26RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEINcoord: 3..65
IPR040387RPM1-interacting protein 4/NOI4PANTHERPTHR33159RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEINcoord: 3..65

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08897-RACarg08897-RAmRNA