Carg05628 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05628
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNADH-ubiquinone oxidoreductase chain
LocationCarg_Chr16: 3445114 .. 3445398 (+)
RNA-Seq ExpressionCarg05628
SyntenyCarg05628
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAACTGTCTGCGGCGGGACGGCGCGGCGGCGCGTTGGGCAGGCGAGGAGTGGGACTTTCTCGCGGCGGATGTGGAGGACGGGGGGAAAAGACAGGGATCCACGGCGGCGTCCACGGAGGTGAAAATTAAGATCACGAAGAGGCAGTTGGAGGAGCTGTTGGGGAAGGTGGATGTTGAGGAGATGTCCGTACAGCAGGTTTTGGCTCAATTGATCAGTGCCGGCGAGTCTCGCCACCGTTCGTGGCGCCCGGTGTTGCAGAGCATACGGGAAGTTGACTGA

mRNA sequence

ATGGGCAACTGTCTGCGGCGGGACGGCGCGGCGGCGCGTTGGGCAGGCGAGGAGTGGGACTTTCTCGCGGCGGATGTGGAGGACGGGGGGAAAAGACAGGGATCCACGGCGGCGTCCACGGAGGTGAAAATTAAGATCACGAAGAGGCAGTTGGAGGAGCTGTTGGGGAAGGTGGATGTTGAGGAGATGTCCGTACAGCAGGTTTTGGCTCAATTGATCAGTGCCGGCGAGTCTCGCCACCGTTCGTGGCGCCCGGTGTTGCAGAGCATACGGGAAGTTGACTGA

Coding sequence (CDS)

ATGGGCAACTGTCTGCGGCGGGACGGCGCGGCGGCGCGTTGGGCAGGCGAGGAGTGGGACTTTCTCGCGGCGGATGTGGAGGACGGGGGGAAAAGACAGGGATCCACGGCGGCGTCCACGGAGGTGAAAATTAAGATCACGAAGAGGCAGTTGGAGGAGCTGTTGGGGAAGGTGGATGTTGAGGAGATGTCCGTACAGCAGGTTTTGGCTCAATTGATCAGTGCCGGCGAGTCTCGCCACCGTTCGTGGCGCCCGGTGTTGCAGAGCATACGGGAAGTTGACTGA

Protein sequence

MGNCLRRDGAAARWAGEEWDFLAADVEDGGKRQGSTAASTEVKIKITKRQLEELLGKVDVEEMSVQQVLAQLISAGESRHRSWRPVLQSIREVD
Homology
BLAST of Carg05628 vs. NCBI nr
Match: KAG6607802.1 (hypothetical protein SDJN03_01144, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015198.1 hypothetical protein SDJN02_22831, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 184.9 bits (468), Expect = 3.2e-43
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 0

Query: 1  MGNCLRRDGAAARWAGEEWDFLAADVEDGGKRQGSTAASTEVKIKITKRQLEELLGKVDV 60
          MGNCLRRDGAAARWAGEEWDFLAADVEDGGKRQGSTAASTEVKIKITKRQLEELLGKVDV
Sbjct: 1  MGNCLRRDGAAARWAGEEWDFLAADVEDGGKRQGSTAASTEVKIKITKRQLEELLGKVDV 60

Query: 61 EEMSVQQVLAQLISAGESRHRSWRPVLQSIREVD 95
          EEMSVQQVLAQLISAGESRHRSWRPVLQSIREVD
Sbjct: 61 EEMSVQQVLAQLISAGESRHRSWRPVLQSIREVD 94

BLAST of Carg05628 vs. NCBI nr
Match: XP_022936225.1 (uncharacterized protein LOC111442898 [Cucurbita moschata] >KAG6592042.1 hypothetical protein SDJN03_14388, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024918.1 hypothetical protein SDJN02_13738, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 137.9 bits (346), Expect = 4.5e-29
Identity = 77/106 (72.64%), Postives = 85/106 (80.19%), Query Frame = 0

Query: 1   MGNCLRRDGAAARWAGEEWDFLAADVEDGGK--------RQGSTAASTEVKIKITKRQLE 60
           MGNCLRRDGAAA+WAGEEWDFLAA  ED G+        ++ S+ A+TEVKIKITKRQLE
Sbjct: 1   MGNCLRRDGAAAQWAGEEWDFLAA--EDDGREGLLGEIEKKKSSTAATEVKIKITKRQLE 60

Query: 61  ELLGKVDVEEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
           ELLGKVD++EMSVQQVLAQLI  G    ESRHR WRPVLQSI EVD
Sbjct: 61  ELLGKVDIKEMSVQQVLAQLIGVGDQFHESRHRHWRPVLQSIPEVD 104

BLAST of Carg05628 vs. NCBI nr
Match: XP_022975730.1 (uncharacterized protein LOC111475920 [Cucurbita maxima])

HSP 1 Score: 134.4 bits (337), Expect = 5.0e-28
Identity = 74/105 (70.48%), Postives = 84/105 (80.00%), Query Frame = 0

Query: 1   MGNCLRRDGAAARWAGEEWDFLAADVEDG-------GKRQGSTAASTEVKIKITKRQLEE 60
           MGNCLRRDGAAA+WAGEEWD LAA+ +DG        +++ S+  +TEVKIKITKRQLEE
Sbjct: 1   MGNCLRRDGAAAQWAGEEWDLLAAE-DDGREGLLGENEKKKSSTTATEVKIKITKRQLEE 60

Query: 61  LLGKVDVEEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
           LLGKVD++EMSVQQVLAQLI  G    ESRHR WRPVLQSI EVD
Sbjct: 61  LLGKVDIKEMSVQQVLAQLIGVGDQFHESRHRHWRPVLQSIPEVD 104

BLAST of Carg05628 vs. NCBI nr
Match: XP_023536165.1 (uncharacterized protein LOC111797410 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 134.4 bits (337), Expect = 5.0e-28
Identity = 75/106 (70.75%), Postives = 84/106 (79.25%), Query Frame = 0

Query: 1   MGNCLRRDGAAARWAGEEWDFLAADVEDGGK--------RQGSTAASTEVKIKITKRQLE 60
           MGNC+RRDGAAA+WAGEEWDFLAA  ED G+        ++ S+ A+TEVKIKITKRQLE
Sbjct: 1   MGNCIRRDGAAAQWAGEEWDFLAA--EDDGREGLLGEIEKKKSSTAATEVKIKITKRQLE 60

Query: 61  ELLGKVDVEEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
           ELLGKVD++EMSVQQVLAQLI       ESRHR WRPVLQSI EVD
Sbjct: 61  ELLGKVDIKEMSVQQVLAQLIGVDDQFHESRHRHWRPVLQSIPEVD 104

BLAST of Carg05628 vs. NCBI nr
Match: XP_022139678.1 (uncharacterized protein LOC111010526 [Momordica charantia])

HSP 1 Score: 129.4 bits (324), Expect = 1.6e-26
Identity = 71/98 (72.45%), Postives = 77/98 (78.57%), Query Frame = 0

Query: 1  MGNCLRRDGAAARWAGEEWDFLAADVEDGGKRQGSTAASTEVKIKITKRQLEELLGKVDV 60
          MGNCLRRDGAA RWAGEEW FLA   E  G     TAA+TEVKIKITK+QLEELLGK DV
Sbjct: 1  MGNCLRRDGAAERWAGEEWGFLAE--EKQGSAAAPTAAATEVKIKITKKQLEELLGKADV 60

Query: 61 EEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
          + +SVQQVLAQLI+ G    ESRHR WRPVLQSI E+D
Sbjct: 61 KGLSVQQVLAQLIAVGDQFHESRHRPWRPVLQSIPEMD 96

BLAST of Carg05628 vs. ExPASy TrEMBL
Match: A0A6J1FD17 (uncharacterized protein LOC111442898 OS=Cucurbita moschata OX=3662 GN=LOC111442898 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.2e-29
Identity = 77/106 (72.64%), Postives = 85/106 (80.19%), Query Frame = 0

Query: 1   MGNCLRRDGAAARWAGEEWDFLAADVEDGGK--------RQGSTAASTEVKIKITKRQLE 60
           MGNCLRRDGAAA+WAGEEWDFLAA  ED G+        ++ S+ A+TEVKIKITKRQLE
Sbjct: 1   MGNCLRRDGAAAQWAGEEWDFLAA--EDDGREGLLGEIEKKKSSTAATEVKIKITKRQLE 60

Query: 61  ELLGKVDVEEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
           ELLGKVD++EMSVQQVLAQLI  G    ESRHR WRPVLQSI EVD
Sbjct: 61  ELLGKVDIKEMSVQQVLAQLIGVGDQFHESRHRHWRPVLQSIPEVD 104

BLAST of Carg05628 vs. ExPASy TrEMBL
Match: A0A6J1IHJ3 (uncharacterized protein LOC111475920 OS=Cucurbita maxima OX=3661 GN=LOC111475920 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.4e-28
Identity = 74/105 (70.48%), Postives = 84/105 (80.00%), Query Frame = 0

Query: 1   MGNCLRRDGAAARWAGEEWDFLAADVEDG-------GKRQGSTAASTEVKIKITKRQLEE 60
           MGNCLRRDGAAA+WAGEEWD LAA+ +DG        +++ S+  +TEVKIKITKRQLEE
Sbjct: 1   MGNCLRRDGAAAQWAGEEWDLLAAE-DDGREGLLGENEKKKSSTTATEVKIKITKRQLEE 60

Query: 61  LLGKVDVEEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
           LLGKVD++EMSVQQVLAQLI  G    ESRHR WRPVLQSI EVD
Sbjct: 61  LLGKVDIKEMSVQQVLAQLIGVGDQFHESRHRHWRPVLQSIPEVD 104

BLAST of Carg05628 vs. ExPASy TrEMBL
Match: A0A6J1CG88 (uncharacterized protein LOC111010526 OS=Momordica charantia OX=3673 GN=LOC111010526 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.8e-27
Identity = 71/98 (72.45%), Postives = 77/98 (78.57%), Query Frame = 0

Query: 1  MGNCLRRDGAAARWAGEEWDFLAADVEDGGKRQGSTAASTEVKIKITKRQLEELLGKVDV 60
          MGNCLRRDGAA RWAGEEW FLA   E  G     TAA+TEVKIKITK+QLEELLGK DV
Sbjct: 1  MGNCLRRDGAAERWAGEEWGFLAE--EKQGSAAAPTAAATEVKIKITKKQLEELLGKADV 60

Query: 61 EEMSVQQVLAQLISAG----ESRHRSWRPVLQSIREVD 95
          + +SVQQVLAQLI+ G    ESRHR WRPVLQSI E+D
Sbjct: 61 KGLSVQQVLAQLIAVGDQFHESRHRPWRPVLQSIPEMD 96

BLAST of Carg05628 vs. ExPASy TrEMBL
Match: A0A5D2DN47 (Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_D01G091100v1 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.2e-16
Identity = 56/109 (51.38%), Postives = 72/109 (66.06%), Query Frame = 0

Query: 1   MGNCLR--RDGAAARWAGEEWDFLAADVEDGGKRQG------------STAASTEVKIKI 60
           MGNCLR  +  ++ RWAG+EW   A+   DG + +G            S +A  EVK+KI
Sbjct: 1   MGNCLRHQQHQSSTRWAGDEWGSSASAANDGDEEKGLLVNNTEIGLNASPSAIHEVKVKI 60

Query: 61  TKRQLEELLGKVDVEEMSVQQVLAQLISAG---ESRHRSWRPVLQSIRE 93
           +K+QLEELLG+VD +E+SVQQVL QLI+AG   E+  RSWRP LQSI E
Sbjct: 61  SKKQLEELLGRVDAKELSVQQVLEQLINAGNQYEANQRSWRPALQSIPE 109

BLAST of Carg05628 vs. ExPASy TrEMBL
Match: A0A0D2NK27 (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_002G088300 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.6e-16
Identity = 55/109 (50.46%), Postives = 71/109 (65.14%), Query Frame = 0

Query: 1   MGNCLR--RDGAAARWAGEEWDFLAADVEDGGKRQG------------STAASTEVKIKI 60
           MGNCLR  +  ++ RWAG+EW   A+   DG + +G            S +A  EVK+KI
Sbjct: 1   MGNCLRHQQHQSSTRWAGDEWGSSASAANDGDEEKGLLVNNPEIGLNASPSAIHEVKVKI 60

Query: 61  TKRQLEELLGKVDVEEMSVQQVLAQLISAG---ESRHRSWRPVLQSIRE 93
           +K+QLEELLG+VD +E+SVQQVL QLI+ G   E+  RSWRP LQSI E
Sbjct: 61  SKKQLEELLGRVDAKELSVQQVLEQLINVGNQYEANQRSWRPALQSIPE 109

BLAST of Carg05628 vs. TAIR 10
Match: AT3G20340.1 (Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress. )

HSP 1 Score: 66.2 bits (160), Expect = 1.6e-11
Identity = 46/116 (39.66%), Postives = 64/116 (55.17%), Query Frame = 0

Query: 1   MGNCLRRDGAAARWAGEEWD-FLAADVED---------GGK------RQGSTAASTEVKI 60
           MGNCLR + +   WAGE+WD F+  D ED          GK         S+  S E+KI
Sbjct: 1   MGNCLRHE-SEMHWAGEDWDEFITEDEEDHHYSSKTTRDGKPVIVTRDSKSSVPSHEIKI 60

Query: 61  KITKRQLEELLGKVDVEEMSVQQ------VLAQLISAGESRHRSWRPVLQSIREVD 95
           ++TK+QL +LL KV+V +++ QQ      +L        ++ R WRPVLQSI EV+
Sbjct: 61  RLTKKQLHDLLSKVNVHDLTFQQQTFSCPILNNRGYEEANQQRLWRPVLQSIPEVN 115

BLAST of Carg05628 vs. TAIR 10
Match: AT4G21920.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G20340.1); Has 40 Blast hits to 40 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 40; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 5.2e-07
Identity = 36/129 (27.91%), Postives = 64/129 (49.61%), Query Frame = 0

Query: 1   MGNCL-RRDGAAARWAGEE------------WDFLAADVEDGGKRQGSTAAST------- 60
           MGNC+   +     W+G++               +  D +DG K  G T+  T       
Sbjct: 1   MGNCICVTEKTTTSWSGDDNGSYNKRRRRRRSTVVHDDNDDGEKLLGETSNVTSTSSSSS 60

Query: 61  ----EVKIKITKRQLEELLGKVDVEEMSVQQVLAQLISAG-----------ESRHRSWRP 95
               E+KI+ITK++LE+L+  + ++ ++ +++L++LI  G            + H+ W+P
Sbjct: 61  SERREIKIRITKKELEDLMRNIGLKSLTAEEILSKLIFEGGDQIGFSAVDVTNHHQPWKP 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6607802.13.2e-43100.00hypothetical protein SDJN03_01144, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022936225.14.5e-2972.64uncharacterized protein LOC111442898 [Cucurbita moschata] >KAG6592042.1 hypothet... [more]
XP_022975730.15.0e-2870.48uncharacterized protein LOC111475920 [Cucurbita maxima][more]
XP_023536165.15.0e-2870.75uncharacterized protein LOC111797410 [Cucurbita pepo subsp. pepo][more]
XP_022139678.11.6e-2672.45uncharacterized protein LOC111010526 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FD172.2e-2972.64uncharacterized protein LOC111442898 OS=Cucurbita moschata OX=3662 GN=LOC1114428... [more]
A0A6J1IHJ32.4e-2870.48uncharacterized protein LOC111475920 OS=Cucurbita maxima OX=3661 GN=LOC111475920... [more]
A0A6J1CG887.8e-2772.45uncharacterized protein LOC111010526 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A5D2DN471.2e-1651.38Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_D01G091100v1 PE=... [more]
A0A0D2NK273.6e-1650.46Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_002G088300 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G20340.11.6e-1139.66Expression of the gene is downregulated in the presence of paraquat, an inducer ... [more]
AT4G21920.15.2e-0727.91unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33647OS01G0793900 PROTEINcoord: 1..93
NoneNo IPR availablePANTHERPTHR33647:SF5OS01G0793900 PROTEINcoord: 1..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05628-RACarg05628-RAmRNA