Carg05261 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05261
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase RNF185
Location	Carg_Chr07: 3812415 .. 3813089 (-)
RNA-Seq Expression	Carg05261
Synteny	Carg05261

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGCGAGTTTGGAGAATCAACAAGCGTGCCAAATCAAAACCCCTCTTCGTCCAGTAACAATGATATTGGCGATTTCGAATGCAATATTTGTTTCGAGTTAGCTCAAGATCCAATTATCACTCTCTGTGGCCATCTCTTTTGCTGGCCTTGTTTGTATAGATGGCTGCATTATCACTCACAATGTCAAGAATGTCCTGTTTGTAAGGCTCTTATTGAGCAGGAGAAATTGATCCCTCTTTATGGTAGGGGAAAGGTACCATCTGACCCGAGATTGAAGAGATATTCTGGTATGGATGTTCCGAGTCGCCCTGCTGGTCAAAGGCCTCAAACAGCACCTCCCCCTGTTGCTAATGACTTCGCAAACTATGGGTTTGGATTCACTGGTGGCTTTATGCCAATGTCAACAGCAAGGATTGGCAACTTCACCTTGGCGACTGCTTTTGGTGGTCTGATCCCATCTTTATTCAATATCCAGTTTCATGGATTTCCTGATGCTACAGTTTATGGGACTACTTCTGGTTACCCCCCCTATGCATTCAACACATTCCATGGGGGTCATGGCCACCATTTTCAGCAACCGACGAGTCAAGGTCAGCATGCTGATAATGTACTGAAAAACCTCCTTTTACTCGTTGGTGTCTTTGTGATTCTTGCCTTGCTTTGGTGGTAG

mRNA sequence

ATGGCGAGCGAGTTTGGAGAATCAACAAGCGTGCCAAATCAAAACCCCTCTTCGTCCAGTAACAATGATATTGGCGATTTCGAATGCAATATTTGTTTCGAGTTAGCTCAAGATCCAATTATCACTCTCTGTGGCCATCTCTTTTGCTGGCCTTGTTTGTATAGATGGCTGCATTATCACTCACAATGTCAAGAATGTCCTGTTTGTAAGGCTCTTATTGAGCAGGAGAAATTGATCCCTCTTTATGGTAGGGGAAAGGTACCATCTGACCCGAGATTGAAGAGATATTCTGGTATGGATGTTCCGAGTCGCCCTGCTGGTCAAAGGCCTCAAACAGCACCTCCCCCTGTTGCTAATGACTTCGCAAACTATGGGTTTGGATTCACTGGTGGCTTTATGCCAATGTCAACAGCAAGGATTGGCAACTTCACCTTGGCGACTGCTTTTGGTGGTCTGATCCCATCTTTATTCAATATCCAGTTTCATGGATTTCCTGATGCTACAGTTTATGGGACTACTTCTGGTTACCCCCCCTATGCATTCAACACATTCCATGGGGGTCATGGCCACCATTTTCAGCAACCGACGAGTCAAGGTCAGCATGCTGATAATGTACTGAAAAACCTCCTTTTACTCGTTGGTGTCTTTGTGATTCTTGCCTTGCTTTGGTGGTAG

Coding sequence (CDS)

ATGGCGAGCGAGTTTGGAGAATCAACAAGCGTGCCAAATCAAAACCCCTCTTCGTCCAGTAACAATGATATTGGCGATTTCGAATGCAATATTTGTTTCGAGTTAGCTCAAGATCCAATTATCACTCTCTGTGGCCATCTCTTTTGCTGGCCTTGTTTGTATAGATGGCTGCATTATCACTCACAATGTCAAGAATGTCCTGTTTGTAAGGCTCTTATTGAGCAGGAGAAATTGATCCCTCTTTATGGTAGGGGAAAGGTACCATCTGACCCGAGATTGAAGAGATATTCTGGTATGGATGTTCCGAGTCGCCCTGCTGGTCAAAGGCCTCAAACAGCACCTCCCCCTGTTGCTAATGACTTCGCAAACTATGGGTTTGGATTCACTGGTGGCTTTATGCCAATGTCAACAGCAAGGATTGGCAACTTCACCTTGGCGACTGCTTTTGGTGGTCTGATCCCATCTTTATTCAATATCCAGTTTCATGGATTTCCTGATGCTACAGTTTATGGGACTACTTCTGGTTACCCCCCCTATGCATTCAACACATTCCATGGGGGTCATGGCCACCATTTTCAGCAACCGACGAGTCAAGGTCAGCATGCTGATAATGTACTGAAAAACCTCCTTTTACTCGTTGGTGTCTTTGTGATTCTTGCCTTGCTTTGGTGGTAG

Protein sequence

MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW

Homology

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BLAST of Carg05261 vs. NCBI nr
Match: XP_022962894.1 (E3 ubiquitin-protein ligase RNF185 [Cucurbita moschata] >XP_023517319.1 E3 ubiquitin-protein ligase RNF185 [Cucurbita pepo subsp. pepo] >KAG6595083.1 E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027098.1 RING finger protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 476.1 bits (1224), Expect = 1.7e-130
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60
           MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH
Sbjct: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60

Query: 61  SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120
           SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND
Sbjct: 61  SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120

Query: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180
           FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA
Sbjct: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180

Query: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 224

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BLAST of Carg05261 vs. NCBI nr
Match: XP_023004082.1 (E3 ubiquitin-protein ligase RNF185 [Cucurbita maxima])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 222/224 (99.11%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60
           MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH
Sbjct: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60

Query: 61  SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120
           SQCQECPVCKALI++EKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND
Sbjct: 61  SQCQECPVCKALIQREKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120

Query: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180
           FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA
Sbjct: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180

Query: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 224

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BLAST of Carg05261 vs. NCBI nr
Match: XP_022949606.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022978947.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_023543222.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >KAG7034387.1 RING finger protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 429.1 bits (1102), Expect = 2.3e-116
Identity = 201/229 (87.77%), Postives = 213/229 (93.01%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSS-----NNDIGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MA+EFGESTSVP+QNPS SS     NND GDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPP 120
           WLH+HSQCQECPVCKAL+++EKL+PLYGRGK PSDPRL RY G+D+P+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPM+ ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           Y PYAFNTFHGGHGHHF QP S+GQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 Y-PYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

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BLAST of Carg05261 vs. NCBI nr
Match: KAG6604228.1 (E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429.1 bits (1102), Expect = 2.3e-116
Identity = 201/229 (87.77%), Postives = 213/229 (93.01%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSS-----NNDIGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MA+EFGESTSVP+QNPS SS     NND GDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 59  MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 118

Query: 61  WLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPP 120
           WLH+HSQCQECPVCKAL+++EKL+PLYGRGK PSDPRL RY G+D+P+RPAGQRPQTAPP
Sbjct: 119 WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 178

Query: 121 PVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPM+ ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 179 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 238

Query: 181 YPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           Y PYAFNTFHGGHGHHF QP S+GQHADNVLKNL LLVGVFVILALLWW
Sbjct: 239 Y-PYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 286

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BLAST of Carg05261 vs. NCBI nr
Match: XP_008440158.1 (PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo] >XP_008440160.1 PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo])

HSP 1 Score: 428.7 bits (1101), Expect = 3.1e-116
Identity = 199/229 (86.90%), Postives = 217/229 (94.76%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSS-----SNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MA+EFGESTS+P++NPS S     +NND GDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPP 120
           WLH+HSQCQECPVCKALI++EKL+PLYGRGK+PSDPRL RY G+D+P+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PV NDFANYGFGFTGGFMPM++ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           Y PYAFNTFHGGHGHHF QP+++GQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 Y-PYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of Carg05261 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.9e-28
Identity = 90/249 (36.14%), Postives = 121/249 (48.59%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSSNND-IGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHY 60
           MASE    T  P++ P+SSSN D    FECNIC + A+D +++LCGHLFCWPCL +WL  
Sbjct: 1   MASE----TKAPSEEPTSSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDT 60

Query: 61  HSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAN 120
               Q CPVCK+ I+  K++P+YGRG   SDPR K      VP RP GQR +  PPP   
Sbjct: 61  RPNNQVCPVCKSAIDGNKVVPIYGRGGDSSDPRKK------VPPRPKGQRSE--PPP--Q 120

Query: 121 DFANYGFGFTGGFMPMSTARIG-NFTLATAFGGLIPSLFNIQF---------------HG 180
            FA + +G  GG M      +  +F + T   GL P +F + F               +G
Sbjct: 121 SFAGFNWGGDGGMMGGGGPNVHFSFGIGTV-NGLFPLMFMLPFIQGIFPLSFVASFFGNG 180

Query: 181 FPDATVYG--------TTSGYPPYAFNTFHGGHGH-HFQQPTSQGQHADNVLKNLLLLVG 224
              A   G           G   +     HG  GH     P S+    +  L N+   +G
Sbjct: 181 NQGAAAAGGGNGGGNDGNDGTHAHTHGHTHGPRGHGESAAPGSRMAQEEEYLSNIFKYIG 234

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BLAST of Carg05261 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.0e-26
Identity = 73/172 (42.44%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 2   ASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYHS 61
           +S  G ++   + N +  + N    FECNIC + A+D +I+LCGHLFCWPCL++WL    
Sbjct: 14  SSSTGGTSGSSSSNGAGDNTNQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRP 73

Query: 62  QCQECPVCKALIEQEKLIPLYGRGKV-PSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 121
             Q CPVCKA I ++K+IPLYGRG     DPR K       P RP GQRP+   P     
Sbjct: 74  NRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQRPE---PENRGG 133

Query: 122 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGT 173
           F  +GFG  GGF    +  IG F       G+  + FNI   G P   V GT
Sbjct: 134 FQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAVPGT 167

[Close]

BLAST of Carg05261 vs. ExPASy Swiss-Prot
Match: Q568Y3 (E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.8e-26
Identity = 81/184 (44.02%), Postives = 99/184 (53.80%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPS----SSSNNDIGD-------FECNICFELAQDPIITLCGHLFC 60
           MAS+ G S S   +N S    S S+N  G+       FECNIC + A+D +I+LCGHLFC
Sbjct: 1   MASK-GPSASASTENSSAGGPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFC 60

Query: 61  WPCLYRWLHYHSQCQECPVCKALIEQEKLIPLYGRGKV-PSDPRLKRYSGMDVPSRPAGQ 120
           WPCL++WL      Q CPVCKA I ++K+IPLYGRG     DPR K       P RP GQ
Sbjct: 61  WPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQ 120

Query: 121 RPQTAPPPVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDAT 173
           RP+   P     F  +GFG  GGF    +  IG F       G+  + FNI   G P   
Sbjct: 121 RPE---PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPA 165

[Close]

BLAST of Carg05261 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 81/184 (44.02%), Postives = 99/184 (53.80%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPS----SSSNNDIGD-------FECNICFELAQDPIITLCGHLFC 60
           MAS+ G S S   +N S    S S+N  G+       FECNIC + A+D +I+LCGHLFC
Sbjct: 1   MASK-GPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFC 60

Query: 61  WPCLYRWLHYHSQCQECPVCKALIEQEKLIPLYGRGKV-PSDPRLKRYSGMDVPSRPAGQ 120
           WPCL++WL      Q CPVCKA I ++K+IPLYGRG     DPR K       P RP GQ
Sbjct: 61  WPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQ 120

Query: 121 RPQTAPPPVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDAT 173
           RP+   P     F  +GFG  GGF    +  IG F       G+  + FNI   G P   
Sbjct: 121 RPE---PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPA 165

[Close]

BLAST of Carg05261 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-25
Identity = 81/184 (44.02%), Postives = 97/184 (52.72%), Query Frame = 0


Query: 1   MASEFGESTSVPNQN-----PSSSSN------NDIGDFECNICFELAQDPIITLCGHLFC 60
           MAS+ G S S   +N     PS SSN           FECNIC + A+D +I+LCGHLFC
Sbjct: 1   MASK-GPSASASTENSNAGGPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFC 60

Query: 61  WPCLYRWLHYHSQCQECPVCKALIEQEKLIPLYGRGKV-PSDPRLKRYSGMDVPSRPAGQ 120
           WPCL++WL      Q CPVCKA I ++K+IPLYGRG     DPR K       P RP GQ
Sbjct: 61  WPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQ 120

Query: 121 RPQTAPPPVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDAT 173
           RP+   P     F  +GFG  GGF    +  IG F       G+  + FNI   G P   
Sbjct: 121 RPE---PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPA 165

[Close]

BLAST of Carg05261 vs. ExPASy TrEMBL
Match: A0A6J1HID1 (E3 ubiquitin-protein ligase RNF185 OS=Cucurbita moschata OX=3662 GN=LOC111463260 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 8.1e-131
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60
           MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH
Sbjct: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60

Query: 61  SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120
           SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND
Sbjct: 61  SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120

Query: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180
           FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA
Sbjct: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180

Query: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 224

[Close]

BLAST of Carg05261 vs. ExPASy TrEMBL
Match: A0A6J1KPE8 (E3 ubiquitin-protein ligase RNF185 OS=Cucurbita maxima OX=3661 GN=LOC111497500 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 5.3e-130
Identity = 222/224 (99.11%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60
           MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH
Sbjct: 1   MASEFGESTSVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH 60

Query: 61  SQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120
           SQCQECPVCKALI++EKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND
Sbjct: 61  SQCQECPVCKALIQREKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAND 120

Query: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180
           FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA
Sbjct: 121 FANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPYA 180

Query: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 FNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 224

[Close]

BLAST of Carg05261 vs. ExPASy TrEMBL
Match: A0A6J1IMG5 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111478748 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.1e-116
Identity = 201/229 (87.77%), Postives = 213/229 (93.01%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSS-----NNDIGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MA+EFGESTSVP+QNPS SS     NND GDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPP 120
           WLH+HSQCQECPVCKAL+++EKL+PLYGRGK PSDPRL RY G+D+P+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPM+ ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           Y PYAFNTFHGGHGHHF QP S+GQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 Y-PYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

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BLAST of Carg05261 vs. ExPASy TrEMBL
Match: A0A6J1GCG3 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC111452948 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.1e-116
Identity = 201/229 (87.77%), Postives = 213/229 (93.01%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSSS-----NNDIGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MA+EFGESTSVP+QNPS SS     NND GDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPP 120
           WLH+HSQCQECPVCKAL+++EKL+PLYGRGK PSDPRL RY G+D+P+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P ANDFANYGFGFTGGFMPM+ ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           Y PYAFNTFHGGHGHHF QP S+GQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 Y-PYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

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BLAST of Carg05261 vs. ExPASy TrEMBL
Match: A0A1S3B021 (E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo OX=3656 GN=LOC103484707 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.5e-116
Identity = 199/229 (86.90%), Postives = 217/229 (94.76%), Query Frame = 0


Query: 1   MASEFGESTSVPNQNPSSS-----SNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MA+EFGESTS+P++NPS S     +NND GDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHYHSQCQECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPP 120
           WLH+HSQCQECPVCKALI++EKL+PLYGRGK+PSDPRL RY G+D+P+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVANDFANYGFGFTGGFMPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PV NDFANYGFGFTGGFMPM++ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPPYAFNTFHGGHGHHFQQPTSQGQHADNVLKNLLLLVGVFVILALLWW 225
           Y PYAFNTFHGGHGHHF QP+++GQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 Y-PYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of Carg05261 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.2e-70
Identity = 130/226 (57.52%), Postives = 167/226 (73.89%), Query Frame = 0


Query: 6   GESTSVPNQNPSSSSNNDI-GDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYHSQCQ 65
           GES++  + + S++  ND  GDFECNICFELAQDPI+TLCGHLFCWPCLYRWLH+HS  Q
Sbjct: 4   GESSTSTSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQ 63

Query: 66  ECPVCKALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPP----VAND 125
           ECPVCKA+++ +KL+PLYGRGK  +DPR KRY G+ +P+RP GQRP+TA PP     A++
Sbjct: 64  ECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAASN 123

Query: 126 FANYGFGFTGGFMP-MSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPPY 185
           F NYG G  GG MP M+T R GNF++   FGGL+PSLFN QFHGF DAT+YG+T GYP  
Sbjct: 124 FFNYGIGLMGGIMPMMATTRFGNFSM--GFGGLLPSLFNFQFHGFHDATLYGSTPGYPYG 183

Query: 186 AF-NTFHGGHGHHFQQPTSQ-GQHADNVLKNLLLLVGVFVILALLW 224
            + N F G      ++P ++ G  +D  LKN+L  VG+ V++ L+W
Sbjct: 184 GYHNGFRGVPPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLIW 227

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BLAST of Carg05261 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.1e-67
Identity = 125/213 (58.69%), Postives = 162/213 (76.06%), Query Frame = 0


Query: 17  SSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYHSQCQECPVCKALIEQE 76
           + ++NND  +FECNIC +LAQDPI+TLCGHLFCWPCLY+WLH HSQ ++CPVCKA+IE++
Sbjct: 11  NDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCKAVIEED 70

Query: 77  KLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVAN-DFA-NYGF-GFTGGF- 136
           +L+PLYGRGK  +DPR K   G++VP+RP+GQRP+TA PP  N  FA ++GF GF GGF 
Sbjct: 71  RLVPLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETAQPPDPNHGFAHHHGFGGFMGGFA 130

Query: 137 MPMSTARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYG--TTSGYPPYAFNTFHGGHG- 196
            PM++AR GN TL+ AFGGLIPSLFN+ FHGFPDA +YG   + G+P    N FHGGH  
Sbjct: 131 APMASARFGNVTLSAAFGGLIPSLFNLHFHGFPDAAMYGAAASGGFPHGFSNPFHGGHSH 190

Query: 197 -HHFQQPTSQGQHADNVLKNLLLLVGVFVILAL 222
            H +Q+ T +    D+ L+ LLL+V V V+ +L
Sbjct: 191 MHSYQRHTGRQGQQDHHLRILLLIVFVVVVFSL 223

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BLAST of Carg05261 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 9.7e-36
Identity = 73/140 (52.14%), Postives = 95/140 (67.86%), Query Frame = 0


Query: 10  SVPNQNPSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYHSQCQECPVC 69
           ++ N+   +S+N     F CNIC ELA++PI+TLCGHLFCWPCLY+WLHYHS+   CPVC
Sbjct: 5   TITNEEDDASNN-----FGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVC 64

Query: 70  KALIEQEKLIPLYGRGKVPSDPRLKRYSGMDVPSRPAGQRPQTAPPPVANDFANYGFGFT 129
           KAL++++ L+PLYG GK  SDPR K  SG+ VP+RPA  R +TA P +  +  ++G  F 
Sbjct: 65  KALVKEDTLVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRL--EQRHHGSSFF 124

Query: 130 GG---FMPMSTA-RIGNFTL 146
           GG   F  M T  R  NF L
Sbjct: 125 GGHSSFAAMPTGLRFSNFLL 137

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BLAST of Carg05261 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 98.2 bits (243), Expect = 8.8e-21
Identity = 46/102 (45.10%), Postives = 60/102 (58.82%), Query Frame = 0


Query: 16  PSSSSNNDIGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYH-------SQCQECPV 75
           P++   N+ G F+CNIC + A DP++TLCGHLFCWPC+Y+WLH              CPV
Sbjct: 31  PTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPV 90

Query: 76  CKALIEQEKLIPLYGRG-KVPSD---PRLKRYSGMDVPSRPA 107
           CK+ I    L+PLYGRG   PS     + +     D+P RPA
Sbjct: 91  CKSNITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPA 132

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BLAST of Carg05261 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 96.7 bits (239), Expect = 2.6e-20
Identity = 42/97 (43.30%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 25  GDFECNICFELAQDPIITLCGHLFCWPCLYRWLHYHSQCQE-------------CPVCKA 84
           GDF+CNIC +  +DP++TLCGHLFCWPC+++W +  +  ++             CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 85  LIEQEKLIPLYGRG-KVPSDPRLKRYSGMDVPSRPAG 108
            + +  L+P+YGRG K P        SG +VPSRP G
Sbjct: 77  DVSEATLVPIYGRGQKAPQ-------SGSNVPSRPTG 106

The following BLAST results are available for this feature:

BLAST of Carg05261 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022962894.1	1.7e-130	100.00	E3 ubiquitin-protein ligase RNF185 [Cucurbita moschata] >XP_023517319.1 E3 ubiqu...	[more]
XP_023004082.1	1.1e-129	99.11	E3 ubiquitin-protein ligase RNF185 [Cucurbita maxima]	[more]
XP_022949606.1	2.3e-116	87.77	E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022978947.1 E3 ...	[more]
KAG6604228.1	2.3e-116	87.77	E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_008440158.1	3.1e-116	86.90	PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo] >XP_008440160.1 PRE...	[more]

BLAST of Carg05261 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	1.9e-28	36.14	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q5ZIR9	4.0e-26	42.44	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]
Q568Y3	6.8e-26	44.02	E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 ...	[more]
Q96GF1	1.2e-25	44.02	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]
Q91YT2	1.5e-25	44.02	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1	[more]

BLAST of Carg05261 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HID1	8.1e-131	100.00	E3 ubiquitin-protein ligase RNF185 OS=Cucurbita moschata OX=3662 GN=LOC111463260...	[more]
A0A6J1KPE8	5.3e-130	99.11	E3 ubiquitin-protein ligase RNF185 OS=Cucurbita maxima OX=3661 GN=LOC111497500 P...	[more]
A0A6J1IMG5	1.1e-116	87.77	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111478...	[more]
A0A6J1GCG3	1.1e-116	87.77	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A1S3B021	1.5e-116	86.90	E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo OX=3656 GN=LOC103484707 PE=4 ...	[more]

BLAST of Carg05261 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23780.1	1.2e-70	57.52	RING/U-box superfamily protein	[more]
AT1G19310.1	2.1e-67	58.69	RING/U-box superfamily protein	[more]
AT1G74990.1	9.7e-36	52.14	RING/U-box superfamily protein	[more]
AT4G27470.1	8.8e-21	45.10	RING membrane-anchor 3	[more]
AT4G28270.1	2.6e-20	43.30	RING membrane-anchor 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 29..69 e-value: 4.7E-7 score: 39.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 29..70 score: 13.241681
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 6..99 e-value: 3.6E-17 score: 64.5
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 27..74 e-value: 2.3E-8 score: 33.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 93..114
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR12313:SF47	RING ZINC FINGER PROTEIN-RELATED	coord: 2..223
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 27..71 e-value: 1.55528E-30 score: 105.079
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 25..82
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..223
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 44..53

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05261-RA	Carg05261-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity