Carg04205 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04205
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Defective in cullin neddylation protein
Location	Carg_Chr01: 772552 .. 776559 (+)
RNA-Seq Expression	Carg04205
Synteny	Carg04205

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAAAGCCGAAGTTTTGTTCTTGCTCTTATATTTCCTCTATTCTCCGATTTGGTGCGATAAAAATCAATCTTCTTCTTTGCTTTCTCATAGTATCTCCTCCATTGCAATCGAATTGCGGAAGAAGAAGAAGATGCGGCGTACTACAAGGAAAACCGGTCAACCAAATTCAACTTCCGTTAATTCTTCCGCCGTGGATCTCTTTCGTTCCGGTATTTGATTTTTTGTTTTCTTTTCCAGAATTCGAATTCTTCTACCTGTGATTATCGCCATGGAAATCGGTCGTCGTGTTGTGTATATGTTGTGGTTAATCGCGTGCTACAAGCATGATTAATTACTTTCTTGATGGTTCTTTTTAAGGAGATTTGTGTCTTTTTGATACTGCGTGTAGAACTATATTAAGATTTTAGTTAGTCTTGAAATAGAGTGAAGGTTCAGTGGGACATGATTTATGATCGTCGTTTTGTGTTTATGTTGTGTATAGAAGTCCCTCCTTTCATTCTTGCTGGTATAAACTACATGATTTATGATCGTTTGTTATTCACAACAATCTTTTAATTCAACTCTCATGAACTTAAGATTCTCAATTTATTCAGCCTCAAGTAAAGCAAGCACGAAAGAGTTGGAACGGATCAATAGCCTATTTTACTCATATGCCAATGCCTCTTCTGGTTTGATCGAGTAAGTCAAATTTTGGCCCATGGAAATATCTCAATTTTCAGCATTGGTAGTTAGGTTTGCGTGGTACAAATATATATTTTTATTCAGTATTATACTGTTTAAATTCCCAGCAATATTCAAATAATGAAAATTTTTCCTTTTGTGGTTGATATCTCACGCTTACTCTTGTATAATAATACTATCCTTCTACATCAAAACTTCCCATCATGTTCTTGTAATGATCAAGAAGGTTGTTTTCCTTATCTTTAAGGAGACTTCTGAATACATGTATAGATAAGATCCATATGGTATGTAGTACTGACTATTTCTTTAACAGTCCAGAAGGAATTGAAACTCTTTGTTCAGACATGGAAGTGGATCACACTGATGTAAGGATCTTGATGCTTGCTTGGTAAGTTGTAGTTAGATTGATACGATAACTCAATATTTTTGTGTCTATGATCCCATTGCCTAGAAACTAATGGATTGCACATACCAGGAAAATGAAAGCTGAAAAACAGGGATACTTTAACCTGGTAAGAATGTTTACTCTCTTCTGGGCCTCCGAATATTATATAAGTTATACAGCAGATCTTCTTTCTCGTATAGTTCCCCTTTATTTTCTGCAAAATGCATCCACATCATTGAATCCTTTCTATTCATTTTCTAATTAGTATGGTAATCTGTAAAGTGTATTTTGTGTGTTGAGCACCAGCTTGTAAGGAAAATTCTTTAGTTTATGACCTAACTTGAACATACACTTCAGTTCTATGGAAAATTTTGTCATTTATATTCATAGCTTCAAATTATTTGGAAAGTTTTGTTTATTGTTTATATATCTTATTTTCCTCTTGTGTTTACTGTTCTCTTCCTTTTCTTTCATCATGCCCATGTTCCATCTTATTATATATTCTAATTTCAGTTTGAGACATGATGAAGCATTTAGTCCCTCGTGCCCCCTCCTTAGTAAATTCATTTTATGGAACCTTAACCTATTGCTTATTCTATAGGAGGAATGGCGTAGAGGCCTCAAATCCCTGAAGGCTGACACTGTTAGTAAACTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTACATTCTTGTCTTTTTAAAAGTTCAAAAATAAACAAGTACATATAGTTGGTGGATTGCTGAATTTTTGCACATAAAGTTCTTAATTATCAATACCTCACATTTTGAGAAGGTAAGGAGGCCATCAAATTTTGTGGACTTCTATTCATATGCATTTCGGTATTGTTTAACAGGTAAACAGATTATTTTAGTGTATTTTTTATTTTGAAGATCGGTTCATTTTCTTCTACTTTATATAATTTGATATTTACGTTTTCTTTAATCCATTAACTTTTTTTTTTTTCTTAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGTATATGCGAGCTGCTGAATCTTGTTTTAGGGTCTCAGTTTCATGCTCAAGTCAATTCATTTGTTGAATACTTGAAGGTGAGTGCTGCACTACGTTTGTGTCATCTAAATTGCCACATTTATCCATGGGCATGGTTCATTTGCCACTGCAAGCGACTCCACCTCTGCCTCAAAGTACTTCTACATATTTAATAGTAGCACTTAGTCTGAGAGAGTTACATTCTTGAGTTTTGGCCTCTCTTTAGCATACATAGTGGAGCACACTGCCCCGTGTCGATAAAATTGACTTTACCATATTTCTTCTGTTAGCTTAAGCCATAGCTTTCTAGGCAATTCGTTTTATGTTAATCTGCTTCTCACAAGAACTTAAGCACATGGGTAACCTTGAAAGATATAGTGTAGAGTAACAAAATTTTCTTGCTTTGATTATTATTTTTTTTTTTTCGGGTTATTGGTTGTTTAATCCCCTGTTTGTTGTCTTCCCCCTCATCTAGGCAGAATAAAATTTATTTCTTATTTGCTCGTATTATGTATGCAGATACAAAGTGACTATAAGGTTATTAATATGGATCAATGGATGGGATTCTTTCGGTTCTGCAATGAGGTATTTTGTCTCTCCCTTTCCCCTCAAAGCACATTAACACAATTCATTATATTTTTGCTTTCCATCTTCCCCACCCAGTAATCACCATGGACCATGGGCTCAATAAGAGCCATGAAAACAATAAAGAGTTTAGAGGGAATAGTTTTAAGACATGATGACCACCTGCCTATGAGTTTTCTTGACAACCAAATGTAGTAGGGTTAGGTAGTTATTTTGTGATAATAATCGCAGTGCACCTACTAGAAACATGCTCTGAATATAAGAAGAGAGTCATTGTCAATCCAATGATCCTATCCCTTTTATTCAATGAGATTGGTATTAAATTTATATATCTATATATACACTATATATATCTAAATGTACAACCTTAACTGAGGTAGGAGAAAAGCCAGTGAAGGACAACACAAATAATATATTTACAAAACTATAATTATACTAAGGACAACACAAATAATATATTTACAAAACTATAATTATACTAATCTAAGAACTATAATATTTATATTTTGCTTGCCTTATGAACCTAAAAATACAGAAACTATTAAATTGCTAATTGGGCAATCTAGGAGGGGATCGACTCATCCTATTCGATTAGGGCCACGTGATAATAAACATAGAAGGAATGTGTTCAAATCATGGTGGCCACCTACTTGGAATTTAATGTTACTATGAATTATCTTAGCAATCAATATGTAGTTGGCAGTTATCTCGTGAGAATAACTTTATCTATCCACCCCCAATTGATTTGTTCGTTATTGATATCCTCAATTCAAGTTGTGACCCATGAATCATGATGATCATTGTGCAGATAAATTTTCCGGACCTGAGCAATTACGATTCGGAACTTGCTTGGCCGTTGATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAACAAAGTTAAAAAACCGAATGTCTTCATCATACATTGTATACTCCCACAGTCGTTGAAGGCAAGTATCCTATTCTCTTACGCACTTCATACTCAATGTACGTTGTTGTTTCCAGCTGACCACATTTATTGAACTTGTTCTTTCTTTCTGCAGGCTCGCTTGGTTGGATTACACATTGCATAGCAGCAGAATTTTCACTTATTATTCCTTCTTTCACTGTTCTAACTTCTTTGAAATCGAGGGTGATTCCCATTCTTATGCTAAAGTTATAATTTCAAACATTCATGGATGATTCTGCTAAAGTTTCCTCCCAAGTCCCCCACCCTTTTTTTTTTTGGTATCCATTCTTCATCATCTTAGCCCTCCAATTGACTTGACTATTTTACTTTTACTTGTAAGAACCAGCTCAAGCTGAAAATTTTCTAACAGGATAAGTGTTGG

mRNA sequence

AGGAAAGCCGAAGTTTTGTTCTTGCTCTTATATTTCCTCTATTCTCCGATTTGGTGCGATAAAAATCAATCTTCTTCTTTGCTTTCTCATAGTATCTCCTCCATTGCAATCGAATTGCGGAAGAAGAAGAAGATGCGGCGTACTACAAGGAAAACCGGTCAACCAAATTCAACTTCCGTTAATTCTTCCGCCGTGGATCTCTTTCGTTCCGCCTCAAGTAAAGCAAGCACGAAAGAGTTGGAACGGATCAATAGCCTATTTTACTCATATGCCAATGCCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAACTCTTTGTTCAGACATGGAAGTGGATCACACTGATGTAAGGATCTTGATGCTTGCTTGGAAAATGAAAGCTGAAAAACAGGGATACTTTAACCTGGAGGAATGGCGTAGAGGCCTCAAATCCCTGAAGGCTGACACTGTTAGTAAACTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTAAGGAGGCCATCAAATTTTGTGGACTTCTATTCATATGCATTTCGGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGTATATGCGAGCTGCTGAATCTTGTTTTAGGGTCTCAGTTTCATGCTCAAGTCAATTCATTTGTTGAATACTTGAAGATACAAAGTGACTATAAGGTTATTAATATGGATCAATGGATGGGATTCTTTCGGTTCTGCAATGAGATAAATTTTCCGGACCTGAGCAATTACGATTCGGAACTTGCTTGGCCGTTGATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAACAAAGCTCGCTTGGTTGGATTACACATTGCATAGCAGCAGAATTTTCACTTATTATTCCTTCTTTCACTGTTCTAACTTCTTTGAAATCGAGGGTGATTCCCATTCTTATGCTAAAGTTATAATTTCAAACATTCATGGATGATTCTGCTAAAGTTTCCTCCCAAGTCCCCCACCCTTTTTTTTTTTGGTATCCATTCTTCATCATCTTAGCCCTCCAATTGACTTGACTATTTTACTTTTACTTGTAAGAACCAGCTCAAGCTGAAAATTTTCTAACAGGATAAGTGTTGG

Coding sequence (CDS)

AGGAAAGCCGAAGTTTTGTTCTTGCTCTTATATTTCCTCTATTCTCCGATTTGGTGCGATAAAAATCAATCTTCTTCTTTGCTTTCTCATAGTATCTCCTCCATTGCAATCGAATTGCGGAAGAAGAAGAAGATGCGGCGTACTACAAGGAAAACCGGTCAACCAAATTCAACTTCCGTTAATTCTTCCGCCGTGGATCTCTTTCGTTCCGCCTCAAGTAAAGCAAGCACGAAAGAGTTGGAACGGATCAATAGCCTATTTTACTCATATGCCAATGCCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAACTCTTTGTTCAGACATGGAAGTGGATCACACTGATGTAAGGATCTTGATGCTTGCTTGGAAAATGAAAGCTGAAAAACAGGGATACTTTAACCTGGAGGAATGGCGTAGAGGCCTCAAATCCCTGAAGGCTGACACTGTTAGTAAACTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTAAGGAGGCCATCAAATTTTGTGGACTTCTATTCATATGCATTTCGGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGTATATGCGAGCTGCTGAATCTTGTTTTAGGGTCTCAGTTTCATGCTCAAGTCAATTCATTTGTTGAATACTTGAAGATACAAAGTGACTATAAGGTTATTAATATGGATCAATGGATGGGATTCTTTCGGTTCTGCAATGAGATAAATTTTCCGGACCTGAGCAATTACGATTCGGAACTTGCTTGGCCGTTGATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAACAAAGCTCGCTTGGTTGGATTACACATTGCATAGCAGCAGAATTTTCACTTATTATTCCTTCTTTCACTGTTCTAACTTCTTTGAAATCGAGGGTGATTCCCATTCTTATGCTAAAGTTATAA

Protein sequence

RKAEVLFLLLYFLYSPIWCDKNQSSSLLSHSISSIAIELRKKKKMRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQSSLGWITHCIAAEFSLIIPSFTVLTSLKSRVIPILMLKL

Homology

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BLAST of Carg04205 vs. NCBI nr
Match: KAG7036533.1 (DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 610.9 bits (1574), Expect = 6.0e-171
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   RKAEVLFLLLYFLYSPIWCDKNQSSSLLSHSISSIAIELRKKKKMRRTTRKTGQPNSTSV 60
           RKAEVLFLLLYFLYSPIWCDKNQSSSLLSHSISSIAIELRKKKKMRRTTRKTGQPNSTSV
Sbjct: 1   RKAEVLFLLLYFLYSPIWCDKNQSSSLLSHSISSIAIELRKKKKMRRTTRKTGQPNSTSV 60

Query: 61  NSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRIL 120
           NSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRIL
Sbjct: 61  NSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRIL 120

Query: 121 MLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFR 180
           MLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFR
Sbjct: 121 MLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFR 180

Query: 181 YCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFC 240
           YCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFC
Sbjct: 181 YCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFC 240

Query: 241 NEINFPDLSNYDSELAWPLILDNFVEWLQAKQSSLGWITHCIAAEFSLIIPSFTVLTSLK 300
           NEINFPDLSNYDSELAWPLILDNFVEWLQAKQSSLGWITHCIAAEFSLIIPSFTVLTSLK
Sbjct: 241 NEINFPDLSNYDSELAWPLILDNFVEWLQAKQSSLGWITHCIAAEFSLIIPSFTVLTSLK 300

Query: 301 SRVIPILMLKL 312
           SRVIPILMLKL
Sbjct: 301 SRVIPILMLKL 311

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BLAST of Carg04205 vs. NCBI nr
Match: XP_022948455.1 (DCN1-like protein 4 [Cucurbita moschata])

HSP 1 Score: 459.9 bits (1182), Expect = 1.7e-125
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE
Sbjct: 1   MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
           TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE
Sbjct: 61  TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD
Sbjct: 121 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

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BLAST of Carg04205 vs. NCBI nr
Match: XP_023523784.1 (DCN1-like protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 457.6 bits (1176), Expect = 8.6e-125
Identity = 227/229 (99.13%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE
Sbjct: 1   MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
           TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE
Sbjct: 61  TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD
Sbjct: 121 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKV+NMDQWMGFFRFCNEI+FPDLSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 YKVVNMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

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BLAST of Carg04205 vs. NCBI nr
Match: XP_022997694.1 (DCN1-like protein 4 [Cucurbita maxima])

HSP 1 Score: 455.7 bits (1171), Expect = 3.3e-124
Identity = 227/229 (99.13%), Postives = 228/229 (99.56%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERIN LFYSYANASSGLIDPEGIE
Sbjct: 1   MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINILFYSYANASSGLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
           TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE
Sbjct: 61  TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD
Sbjct: 121 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKVINMDQWMGFFRFCNEI+FPDLSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 YKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

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BLAST of Carg04205 vs. NCBI nr
Match: KAG6606827.1 (DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.4 bits (1134), Expect = 6.3e-120
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE
Sbjct: 1   MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
           TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE
Sbjct: 61  TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD
Sbjct: 121 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNF 265
           YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNF
Sbjct: 181 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNF 220

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BLAST of Carg04205 vs. ExPASy Swiss-Prot
Match: Q92564 (DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 8.5e-35
Identity = 96/257 (37.35%), Postives = 138/257 (53.70%), Query Frame = 0


Query: 20  DKNQSSSLLSHSISSIAIELRKKKKMRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKE 79
           D +Q+ SL S S S    ++   +K RR           S + S    + S   K   + 
Sbjct: 41  DDHQTGSLRSCSSSDCFNKVMPPRKKRRPASGDDLSAKKSRHDSMYRKYDSTRIKTEEEA 100

Query: 80  L--ERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLE 139
              +R    FY YA  +  ++ PEG+E  C D+ V+  +V +L+LAWK+ A+  GYF L+
Sbjct: 101 FSSKRCLEWFYEYA-GTDDVVGPEGMEKFCEDIGVEPENVVMLVLAWKLDAQNMGYFTLQ 160

Query: 140 EWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESIC 199
           EW +G+ SL+ DT  KL+  L  L   +   +NF   Y YAF +   E+ Q+S+DI +  
Sbjct: 161 EWLKGMTSLQCDTTEKLRNTLDYLRSFLNDSTNFKLIYRYAFDFA-REKDQRSLDINTAK 220

Query: 200 ELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAW 259
            +L L+LG +       F ++L+ QS YKVIN DQW     F   IN  DLSNYD + AW
Sbjct: 221 CMLGLLLG-KIWPLFPVFHQFLE-QSKYKVINKDQWCNVLEFSRTINL-DLSNYDEDGAW 280

Query: 260 PLILDNFVEWLQAKQSS 275
           P++LD FVEW + KQ S
Sbjct: 281 PVLLDEFVEWYKDKQMS 292

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BLAST of Carg04205 vs. ExPASy Swiss-Prot
Match: Q8CCA0 (DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.5e-34
Identity = 95/257 (36.96%), Postives = 136/257 (52.92%), Query Frame = 0


Query: 20  DKNQSSSLLSHSISSIAIELRKKKKMRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKE 79
           D +Q+ SL S S S    ++   +K RR           S + S    + S   K   + 
Sbjct: 41  DDHQTGSLRSCSSSDCFSKVMPPRKKRRPASGDDLSAKKSRHDSMYRKYESTRIKTEEEA 100

Query: 80  L--ERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLE 139
              +R    FY YA      + PEG+E  C D+ V+  +V +L+LAWK+ A+  GYF L+
Sbjct: 101 FSSKRCLEWFYEYAGTEDA-VGPEGMEKFCEDIGVEPENVVMLVLAWKLDAQNMGYFTLQ 160

Query: 140 EWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESIC 199
           EW +G+ SL+ DT  KL+  L  L   +   +NF   Y YAF +   E+ Q+S+DI +  
Sbjct: 161 EWLKGMTSLQCDTTEKLRTTLDYLRSLLNDTTNFKLIYRYAFDFA-REKDQRSLDINTAK 220

Query: 200 ELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAW 259
            +L L+LG +       F ++L+ QS YKVIN DQW     F   I+  DLSNYD + AW
Sbjct: 221 CMLGLLLG-KIWPLFPVFHQFLE-QSKYKVINKDQWCNVLEFSRTISL-DLSNYDEDGAW 280

Query: 260 PLILDNFVEWLQAKQSS 275
           P++LD FVEW + KQ S
Sbjct: 281 PVLLDEFVEWYKDKQMS 292

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BLAST of Carg04205 vs. ExPASy Swiss-Prot
Match: Q9BTE7 (DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 73/188 (38.83%), Postives = 116/188 (61.70%), Query Frame = 0


Query: 87  FYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSL 146
           FY YA     ++ PEG+E  C D+ V+  ++ +L+LAWK++AE  G+F  EEW +G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 147 KADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGS 206
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 207 QFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVE 266
            +    + F +YL+ QS Y+V+N DQW     F   ++  DLSNYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTVH-ADLSNYDEDGAWPVLLDEFVE 234

Query: 267 WLQAKQSS 275
           W + +Q+S
Sbjct: 235 WQKVRQTS 237

[Close]

BLAST of Carg04205 vs. ExPASy Swiss-Prot
Match: Q1RMX9 (DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-33
Identity = 73/188 (38.83%), Postives = 116/188 (61.70%), Query Frame = 0


Query: 87  FYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSL 146
           FY YA     ++ PEG+E  C D+ V+  ++ +L+LAWK++AE  G+F  EEW +G+ SL
Sbjct: 54  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 113

Query: 147 KADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGS 206
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 114 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 173

Query: 207 QFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVE 266
            +    + F +YL+ QS Y+V+N DQW     F   ++  DLSNYD + AWP++LD FVE
Sbjct: 174 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTVH-ADLSNYDEDGAWPVLLDEFVE 233

Query: 267 WLQAKQSS 275
           W + +Q+S
Sbjct: 234 WHKVRQAS 236

[Close]

BLAST of Carg04205 vs. ExPASy Swiss-Prot
Match: Q9CXV9 (DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.7e-33
Identity = 73/188 (38.83%), Postives = 116/188 (61.70%), Query Frame = 0


Query: 87  FYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSL 146
           FY YA     ++ PEG+E  C D+ V+  ++ +L+LAWK++AE  G+F  EEW +G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 147 KADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGS 206
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQSRFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 207 QFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVE 266
            +    + F +YL+ QS Y+V+N DQW     F   ++  DLSNYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTVH-ADLSNYDEDGAWPVLLDEFVE 234

Query: 267 WLQAKQSS 275
           W + +Q+S
Sbjct: 235 WQKIRQTS 237

[Close]

BLAST of Carg04205 vs. ExPASy TrEMBL
Match: A0A6J1G9W7 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111452134 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 8.3e-126
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE
Sbjct: 1   MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
           TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE
Sbjct: 61  TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD
Sbjct: 121 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

[Close]

BLAST of Carg04205 vs. ExPASy TrEMBL
Match: A0A6J1KCA5 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111492580 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.6e-124
Identity = 227/229 (99.13%), Postives = 228/229 (99.56%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERIN LFYSYANASSGLIDPEGIE
Sbjct: 1   MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINILFYSYANASSGLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
           TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE
Sbjct: 61  TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD
Sbjct: 121 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKVINMDQWMGFFRFCNEI+FPDLSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 YKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

[Close]

BLAST of Carg04205 vs. ExPASy TrEMBL
Match: A0A0A0KYL5 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G006480 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 8.9e-120
Identity = 223/255 (87.45%), Postives = 241/255 (94.51%), Query Frame = 0


Query: 22  NQSSSLLSHSISSIAIEL--RKKKKMRRT-TRKTGQPNSTSVNSSAVDLFRSASSKASTK 81
           + SSSLLS  IS IAI+    K KKMRR+ TRKTGQPNSTSVNSSAVDLFRSASS+AS+K
Sbjct: 67  SSSSSLLSLLISFIAIQFFKNKNKKMRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSK 126

Query: 82  ELERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEE 141
           ELERI+SLFYSYAN SSGLIDPEGIE LCSD+EVDHTDVRILMLAWKMK+EKQGYFNL+E
Sbjct: 127 ELERIDSLFYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDE 186

Query: 142 WRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICE 201
           WR GLKSL+ADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICE
Sbjct: 187 WRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICE 246

Query: 202 LLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAWP 261
           LL+LVLGSQFH+QVN+FV+YLKIQ+DYKVINMDQWMGFFRFCNEI++PDL NYDSELAWP
Sbjct: 247 LLDLVLGSQFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWP 306

Query: 262 LILDNFVEWLQAKQS 274
           LILDNFVEWLQAKQ+
Sbjct: 307 LILDNFVEWLQAKQN 321

[Close]

BLAST of Carg04205 vs. ExPASy TrEMBL
Match: A0A6J1DKL7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111020935 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 7.1e-117
Identity = 210/228 (92.11%), Postives = 224/228 (98.25%), Query Frame = 0


Query: 46  RRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIET 105
           R  TRKTGQPNSTSVNSSAVDLFRSASS+ASTKELERI+SL+YSYAN+SSGLIDPEGIET
Sbjct: 3   RSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGIET 62

Query: 106 LCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEV 165
           LCSDMEV+HTDVRILMLAWKMK+EKQGYFNLEEWRRGLKSL+ADTVSKLKKALPDLEKEV
Sbjct: 63  LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEV 122

Query: 166 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDY 225
           RRPSNF DFYSYAF+YCLTEEKQKSIDIESICELL+LVLGSQFH+QVN+FVEYLK+QSDY
Sbjct: 123 RRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDY 182

Query: 226 KVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           KVINMDQWMGFFRFCNEI++PDLSNYDSELAWPLILDNFVEWL AKQS
Sbjct: 183 KVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

[Close]

BLAST of Carg04205 vs. ExPASy TrEMBL
Match: A0A1S4DZM9 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.6e-116
Identity = 209/228 (91.67%), Postives = 223/228 (97.81%), Query Frame = 0


Query: 46  RRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIET 105
           R  TRKTGQPNSTSVNSSAVDLFRSASS+ASTKELERI+SLFYSYAN +SGLIDPEGIE 
Sbjct: 3   RSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGIEN 62

Query: 106 LCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEV 165
           LCSD+EVDHTDVRILMLAWKMK+EKQGYFNL+EWR GLKSL+ADTVSKLKKALPDLEKEV
Sbjct: 63  LCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEKEV 122

Query: 166 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDY 225
           RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELL+LVLGSQFH+QVN+FVEYLKIQ+DY
Sbjct: 123 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQTDY 182

Query: 226 KVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           KVINMDQWMGFFRFCNEI++PDLSNYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 183 KVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

[Close]

BLAST of Carg04205 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 350.5 bits (898), Expect = 1.4e-96
Identity = 169/229 (73.80%), Postives = 198/229 (86.46%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRR++ K  + +  S  S   DLFRSASSKAS KE++RI+ LF  YAN SS LIDPEGIE
Sbjct: 1   MRRSSSK--KKSGQSTESVTTDLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
            LCS++EV HTD+RILMLAWKMKAEKQGYF  EEWRRGLK+L+ADT++KLKKALP+LEKE
Sbjct: 61  ELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL +V+GS F AQV+ FVEYLKIQ+D
Sbjct: 121 VRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQND 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKVINMDQWMG +RFCNEI+FPD+ +Y+ ELAWPLILDNFVEW+Q KQ+
Sbjct: 181 YKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 227

[Close]

BLAST of Carg04205 vs. TAIR 10
Match: AT1G15860.2 (Domain of unknown function (DUF298) )

HSP 1 Score: 350.5 bits (898), Expect = 1.4e-96
Identity = 169/229 (73.80%), Postives = 198/229 (86.46%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRR++ K  + +  S  S   DLFRSASSKAS KE++RI+ LF  YAN SS LIDPEGIE
Sbjct: 1   MRRSSSK--KKSGQSTESVTTDLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
            LCS++EV HTD+RILMLAWKMKAEKQGYF  EEWRRGLK+L+ADT++KLKKALP+LEKE
Sbjct: 61  ELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSD 224
           VRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL +V+GS F AQV+ FVEYLKIQ+D
Sbjct: 121 VRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQND 180

Query: 225 YKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
           YKVINMDQWMG +RFCNEI+FPD+ +Y+ ELAWPLILDNFVEW+Q KQ+
Sbjct: 181 YKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 227

[Close]

BLAST of Carg04205 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 342.8 bits (878), Expect = 2.9e-94
Identity = 169/238 (71.01%), Postives = 198/238 (83.19%), Query Frame = 0


Query: 45  MRRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIE 104
           MRR++ K  + +  S  S   DLFRSASSKAS KE++RI+ LF  YAN SS LIDPEGIE
Sbjct: 1   MRRSSSK--KKSGQSTESVTTDLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGIE 60

Query: 105 TLCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKE 164
            LCS++EV HTD+RILMLAWKMKAEKQGYF  EEWRRGLK+L+ADT++KLKKALP+LEKE
Sbjct: 61  ELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEKE 120

Query: 165 VRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLK---- 224
           VRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL +V+GS F AQV+ FVEYLK    
Sbjct: 121 VRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWIT 180

Query: 225 -----IQSDYKVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 274
                IQ+DYKVINMDQWMG +RFCNEI+FPD+ +Y+ ELAWPLILDNFVEW+Q KQ+
Sbjct: 181 QKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 236

[Close]

BLAST of Carg04205 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 118.6 bits (296), Expect = 8.8e-27
Identity = 67/210 (31.90%), Postives = 120/210 (57.14%), Query Frame = 0


Query: 63  SAVDLFRS-ASSKASTKELERINSLFYSYANASSGLIDPEGIETLCSDMEVDHTDVRILM 122
           +A D+F S    +++  E+ R+  L+  Y +  S +I  EGI  LC+D+EV+  D+  L+
Sbjct: 39  AAFDVFYSQPQPRSNAAEVRRLEELYNRYKDPYSDMILAEGISVLCNDLEVEPQDIVTLV 98

Query: 123 LAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRY 182
           L+W M A     F+ +E+  GL++L  D++ KL++ LP +  E++    F + Y++AF +
Sbjct: 99  LSWHMNAATACEFSRQEFISGLQALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGW 158

Query: 183 CLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDYKVINMDQWMGFFRFCN 242
              E+ QKS+ +++   +  L+   +    V  + ++L+ + + K I+ D W     F  
Sbjct: 159 A-KEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDRHN-KAISKDTWAQLLEFSR 218

Query: 243 EINFPDLSNYDSELAWPLILDNFVEWLQAK 272
            ++ P LSNYD+E AWP ++D FVE+L  K
Sbjct: 219 MVD-PVLSNYDAEGAWPYLIDEFVEYLYDK 245

[Close]

BLAST of Carg04205 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 51.2 bits (121), Expect = 1.7e-06
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = 0


Query: 169 SNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDYKVI 228
           + F  FY + F  C  E  QK+I I        LVL  +F   +N + ++++    +  I
Sbjct: 84  TEFTCFYDFVFFMC-RENGQKNITISRAITAWKLVLAGRFRL-LNRWCDFIEKNQRHN-I 143

Query: 229 NMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVE 267
           + D W     F   ++  +L  YDSE AWP+++D+FVE
Sbjct: 144 SEDTWQQVLAFSRCVH-ENLEGYDSEGAWPVLIDDFVE 177

The following BLAST results are available for this feature:

BLAST of Carg04205 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036533.1	6.0e-171	100.00	DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022948455.1	1.7e-125	100.00	DCN1-like protein 4 [Cucurbita moschata]	[more]
XP_023523784.1	8.6e-125	99.13	DCN1-like protein 4 [Cucurbita pepo subsp. pepo]	[more]
XP_022997694.1	3.3e-124	99.13	DCN1-like protein 4 [Cucurbita maxima]	[more]
KAG6606827.1	6.3e-120	100.00	DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg04205 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q92564	8.5e-35	37.35	DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2	[more]
Q8CCA0	2.5e-34	36.96	DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1	[more]
Q9BTE7	2.1e-33	38.83	DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1	[more]
Q1RMX9	2.7e-33	38.83	DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1	[more]
Q9CXV9	2.7e-33	38.83	DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1	[more]

BLAST of Carg04205 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G9W7	8.3e-126	100.00	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KCA5	1.6e-124	99.13	Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A0A0KYL5	8.9e-120	87.45	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G0064...	[more]
A0A6J1DKL7	7.1e-117	92.11	Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A1S4DZM9	1.6e-116	91.67	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 ...	[more]

BLAST of Carg04205 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15860.1	1.4e-96	73.80	Domain of unknown function (DUF298)	[more]
AT1G15860.2	1.4e-96	73.80	Domain of unknown function (DUF298)	[more]
AT1G15860.3	2.9e-94	71.01	Domain of unknown function (DUF298)	[more]
AT3G12760.1	8.8e-27	31.90	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]
AT3G28970.1	1.7e-06	31.63	Domain of unknown function (DUF298)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 153..267 e-value: 4.4E-27 score: 94.9
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 78..269 score: 42.659851
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 168..274 e-value: 2.2E-28 score: 100.6
None	No IPR available	GENE3D	1.10.238.10		coord: 76..166 e-value: 4.0E-29 score: 102.4
None	No IPR available	PANTHER	PTHR12281:SF12	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 48..272
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 48..272
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 71..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04205-RA	Carg04205-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding