Carg03498 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg03498 vs. NCBI nr
Match: KAG7017628.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526.9 bits (1356), Expect = 1.0e-145
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA
Sbjct: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT
Sbjct: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 270

BLAST of Carg03498 vs. NCBI nr
Match: XP_023527312.1 (WAT1-related protein At5g07050-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.5 bits (1334), Expect = 3.5e-143
Identity = 267/270 (98.89%), Postives = 268/270 (99.26%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA
Sbjct: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLA LGPLIDQNFYYLGIKMTSPTFACAISNT+PSMT
Sbjct: 61  PFALVFERKMRPKITLKIFAQLFVLAFLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 181 AD-AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 269

BLAST of Carg03498 vs. NCBI nr
Match: XP_023528520.1 (WAT1-related protein At5g07050-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.5 bits (1334), Expect = 3.5e-143
Identity = 267/270 (98.89%), Postives = 268/270 (99.26%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA
Sbjct: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLA LGPLIDQNFYYLGIKMTSPTFACAISNT+PSMT
Sbjct: 61  PFALVFERKMRPKITLKIFAQLFVLAFLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 181 AD-AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 269

BLAST of Carg03498 vs. NCBI nr
Match: KAG6580872.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 518.1 bits (1333), Expect = 4.6e-143
Identity = 266/270 (98.52%), Postives = 268/270 (99.26%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHY+LVVYRHAFATVVMA
Sbjct: 22  MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYILVVYRHAFATVVMA 81

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNT+PSMT
Sbjct: 82  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 141

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 142 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 201

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 202 AD-AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 261

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVT AMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 262 QAIVVTFAMEHRPHAWAIGWDMNLLAAAYA 290

BLAST of Carg03498 vs. NCBI nr
Match: XP_022934813.1 (WAT1-related protein At5g07050-like [Cucurbita moschata])

HSP 1 Score: 516.9 bits (1330), Expect = 1.0e-142
Identity = 266/270 (98.52%), Postives = 267/270 (98.89%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGY GMNIISAISLNHGMSHYVLVVYRHAFATVVMA
Sbjct: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNT+PSMT
Sbjct: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 181 AD-AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVT AMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTFAMEHRPHAWAIGWDMNLLAAAYA 269

BLAST of Carg03498 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 3.7e-87
Identity = 166/276 (60.14%), Postives = 205/276 (74.28%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           M  + S    L ++KPY  +ISLQFGYAGMNII+ ISLN GMSHYVLVVYRHA AT V+A
Sbjct: 3   MEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIA 62

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFA  FERK +PKIT  IF QLF+L LLGP+IDQNFYY+G+K TSPTF+CA+SN +P+MT
Sbjct: 63  PFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMT 122

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGH-----GRHP 180
           FI+A+L RME L+LK + CQAK+ GT+VT+ GAMLMT YKG ++  FWT +       H 
Sbjct: 123 FILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHA 182

Query: 181 NTPPTADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAH-LSLTTMV 240
           NT        ++ N  +D + +KGSI LI ATLAWA+ F+LQ   ++ Y  H LSLTT++
Sbjct: 183 NT-------TSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLI 242

Query: 241 CFLGTLQAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           CF+GTLQA+ VT  MEH P AW IGWDMNLLAAAY+
Sbjct: 243 CFIGTLQAVAVTFVMEHNPSAWRIGWDMNLLAAAYS 271

BLAST of Carg03498 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.7e-77
Identity = 144/259 (55.60%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGYAGMN+++   L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRMEK 131
            K+T  IF ++F+LALLGP+IDQN YY+G+K+TSPTF+ A+SN +P++T I+A L RMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASNLH 191
           +E++ VRC  KV GT+VT+VG++LM FYKG  INFF   H    ++PPTAD         
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTAD--------- 186

Query: 192 NDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAMEH 251
                +K ++FL++A+L+WA+FF+LQ  T++KY+AHLS++TMVCF+GTLQ++ +   MEH
Sbjct: 187 ----YLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEH 246

Query: 252 RPHAWAIGWDMNLLAAAYA 271
            P A  IG+DMNLLA+AYA
Sbjct: 247 NPSALNIGFDMNLLASAYA 251

BLAST of Carg03498 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.6e-74
Identity = 141/259 (54.44%), Postives = 195/259 (75.29%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGYAGMN+++ + L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRMEK 131
           PK+T  IF Q+FVLALLGPLIDQN YY G+K+TSPTFA A++N +P++TFI++++CRMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASNLH 191
           +E++ VR QAKV GT+V +VGAMLM  +K  +I F  +    H  +P   D         
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAGED--------- 186

Query: 192 NDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAMEH 251
                +K ++FL+IA+ +WA+FF+LQ  T+++Y++HLSL+TMVCF+GTLQ+  +T  ME 
Sbjct: 187 ----YLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEP 246

Query: 252 RPHAWAIGWDMNLLAAAYA 271
              AW IG+DMNLLA+AYA
Sbjct: 247 NLSAWNIGFDMNLLASAYA 252

BLAST of Carg03498 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 6.9e-65
Identity = 126/261 (48.28%), Postives = 177/261 (67.82%), Query Frame = 0

Query: 10  LLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERK 69
           L+ + KPY+ +IS+QFGYAGM II+ +SL HGM+HYVL VYRHA AT V+APFAL  ERK
Sbjct: 5   LMNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERK 64

Query: 70  MRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRM 129
           +RPK+T +IF Q+ +L  + P++DQN YY+G+  TS TFA A +N +P++TF++A++ R+
Sbjct: 65  IRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRL 124

Query: 130 EKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASN 189
           E +  K VR  AKV GT++T+ GA+LMT YKG +++F   G G    +     +   A  
Sbjct: 125 ESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGA 184

Query: 190 LHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAM 249
              D   I G++ L+  T  WA FFILQ  T+++Y A LSLTT++C +GTL+   V+L  
Sbjct: 185 AAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVT 244

Query: 250 EHRPHAWAIGWDMNLLAAAYA 271
                AW IG+D NL AAAY+
Sbjct: 245 VRDLSAWKIGFDSNLFAAAYS 265

BLAST of Carg03498 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.0e-61
Identity = 125/261 (47.89%), Postives = 178/261 (68.20%), Query Frame = 0

Query: 11  LQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKM 70
           ++ A+P+I ++ LQ G AGM+I+S   LN GMS+YVLVVYRHA AT+VMAPFA  F++K+
Sbjct: 10  MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKV 69

Query: 71  RPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRME 130
           RPK+TL IF ++ +L LL P+IDQN YYLG+K T+ TFA A+ N +P++TF++A +  +E
Sbjct: 70  RPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLE 129

Query: 131 KLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASNL 190
           +++L+ +R   KV GT+ T+ GAM+MT  KG V++ FWT      NT        A +++
Sbjct: 130 RVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNT--------AGTDI 189

Query: 191 HNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAME 250
           H+    IKG++ + I   ++A F ILQ IT+R Y A LSLT  +C +GT++   V L ME
Sbjct: 190 HS---AIKGAVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVME 249

Query: 251 H-RPHAWAIGWDMNLLAAAYA 271
              P AWAIGWD  LL A Y+
Sbjct: 250 KGNPSAWAIGWDTKLLTATYS 259

BLAST of Carg03498 vs. ExPASy TrEMBL
Match: A0A6J1F8T0 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111441872 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.0e-143
Identity = 266/270 (98.52%), Postives = 267/270 (98.89%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGY GMNIISAISLNHGMSHYVLVVYRHAFATVVMA
Sbjct: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNT+PSMT
Sbjct: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 181 AD-AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVT AMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTFAMEHRPHAWAIGWDMNLLAAAYA 269

BLAST of Carg03498 vs. ExPASy TrEMBL
Match: A0A6J1J8I7 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482309 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.5e-139
Identity = 261/270 (96.67%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQ+AKPYIVVISLQFGYAGMNIISAISL+ GMSHYVLVVYRHAFATV+MA
Sbjct: 1   MGYLQSLGCLLQSAKPYIVVISLQFGYAGMNIISAISLDRGMSHYVLVVYRHAFATVIMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALVFERKM PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNT+PSMT
Sbjct: 61  PFALVFERKMIPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTP T
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPLT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL
Sbjct: 181 AD-AAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAI+VTLAMEHRPHAWAIGWDMNLLAAAYA
Sbjct: 241 QAIIVTLAMEHRPHAWAIGWDMNLLAAAYA 269

BLAST of Carg03498 vs. ExPASy TrEMBL
Match: A0A6J1F9X7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111442149 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.9e-124
Identity = 231/269 (85.87%), Postives = 245/269 (91.08%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGY QS GC  QNA PYIVVISL FGYAGMNIIS ISL+ GMSHYVLVVYRHAFAT VMA
Sbjct: 1   MGYSQSFGCFFQNAMPYIVVISLTFGYAGMNIISTISLDRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFAL FERK+RPKIT KIF QLFVLALLGP+IDQN YYLG+KMTSPTF+CAI N +PSMT
Sbjct: 61  PFALFFERKVRPKITFKIFVQLFVLALLGPVIDQNLYYLGLKMTSPTFSCAIGNMLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMA+LCRMEKL+LK+VRCQAKV GTIVT+VGAMLMTFYKGSVINFFWTGH RHPNTPPT
Sbjct: 121 FIMAVLCRMEKLDLKNVRCQAKVLGTIVTVVGAMLMTFYKGSVINFFWTGHVRHPNTPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AAAASN HNDGK IKGSI LIIATLAWAAFFILQ IT+RKYTAHLSLTTMVCFLGTL
Sbjct: 181 AD-AAAASNPHNDGKFIKGSILLIIATLAWAAFFILQAITLRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAY 270
           QAIVVTLAM+H  H+W+IGWDMNLLAAAY
Sbjct: 241 QAIVVTLAMDHPRHSWSIGWDMNLLAAAY 268

BLAST of Carg03498 vs. ExPASy TrEMBL
Match: A0A6J1EZ96 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111440914 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.0e-119
Identity = 222/270 (82.22%), Postives = 244/270 (90.37%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MG+++ LG   QNA+PYI VISLQFGYAGMNIIS +SLN GMSHYVLVVYRHAFAT VMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALV ERK+RPKIT KIF Q+FVLALLGPLIDQNFYY+G+KMTSPT++CAISN +PSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMA+LCRMEKL+L+ VRCQAK+FGTIVT++GAMLMTFYKGSVINFFWT    HPN+PPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           AD AA   N H+DG+ IKGSI LIIATLAWA FFILQVIT+RKYTAHLSLTTMVCFLGTL
Sbjct: 181 ADTAAL--NHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVTLAMEHRP AWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg03498 vs. ExPASy TrEMBL
Match: A0A6J1I534 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469993 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.3e-119
Identity = 222/270 (82.22%), Postives = 244/270 (90.37%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MG+++ LG   QNA+PYI VISLQFGYAGMNIIS +SLN GMSHYVLVVYRHAFAT VMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFALV ERK+RPKIT KIF Q+FVLALLGPLIDQNFYY+G+KMTSPT++CAISN +PSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMA+LCRMEKL+L+ VRCQAK+FGTIVT++GAMLMTFYKGSVINFFWT   RHPN+PPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQARHPNSPPT 180

Query: 181 ADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTL 240
           ADA A   N H+D + IKGSI LIIATLAWA FFILQVIT+RKYTAHLSLTTMVCFLGTL
Sbjct: 181 ADAGAL--NHHHDREFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTL 240

Query: 241 QAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           QAIVVTLAMEHRP AWAIGWDMNLLAAAYA
Sbjct: 241 QAIVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg03498 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 322.8 bits (826), Expect = 2.7e-88
Identity = 166/276 (60.14%), Postives = 205/276 (74.28%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           M  + S    L ++KPY  +ISLQFGYAGMNII+ ISLN GMSHYVLVVYRHA AT V+A
Sbjct: 3   MEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIA 62

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMT 120
           PFA  FERK +PKIT  IF QLF+L LLGP+IDQNFYY+G+K TSPTF+CA+SN +P+MT
Sbjct: 63  PFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMT 122

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGH-----GRHP 180
           FI+A+L RME L+LK + CQAK+ GT+VT+ GAMLMT YKG ++  FWT +       H 
Sbjct: 123 FILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHA 182

Query: 181 NTPPTADAAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAH-LSLTTMV 240
           NT        ++ N  +D + +KGSI LI ATLAWA+ F+LQ   ++ Y  H LSLTT++
Sbjct: 183 NT-------TSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLI 242

Query: 241 CFLGTLQAIVVTLAMEHRPHAWAIGWDMNLLAAAYA 271
           CF+GTLQA+ VT  MEH P AW IGWDMNLLAAAY+
Sbjct: 243 CFIGTLQAVAVTFVMEHNPSAWRIGWDMNLLAAAYS 271

BLAST of Carg03498 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 290.0 bits (741), Expect = 1.9e-78
Identity = 144/259 (55.60%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGYAGMN+++   L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRMEK 131
            K+T  IF ++F+LALLGP+IDQN YY+G+K+TSPTF+ A+SN +P++T I+A L RMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASNLH 191
           +E++ VRC  KV GT+VT+VG++LM FYKG  INFF   H    ++PPTAD         
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTAD--------- 186

Query: 192 NDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAMEH 251
                +K ++FL++A+L+WA+FF+LQ  T++KY+AHLS++TMVCF+GTLQ++ +   MEH
Sbjct: 187 ----YLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEH 246

Query: 252 RPHAWAIGWDMNLLAAAYA 271
            P A  IG+DMNLLA+AYA
Sbjct: 247 NPSALNIGFDMNLLASAYA 251

BLAST of Carg03498 vs. TAIR 10
Match: AT3G56620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.2e-75
Identity = 141/259 (54.44%), Postives = 195/259 (75.29%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGYAGMN+++ + L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRMEK 131
           PK+T  IF Q+FVLALLGPLIDQN YY G+K+TSPTFA A++N +P++TFI++++CRMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASNLH 191
           +E++ VR QAKV GT+V +VGAMLM  +K  +I F  +    H  +P   D         
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAGED--------- 186

Query: 192 NDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAMEH 251
                +K ++FL+IA+ +WA+FF+LQ  T+++Y++HLSL+TMVCF+GTLQ+  +T  ME 
Sbjct: 187 ----YLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEP 246

Query: 252 RPHAWAIGWDMNLLAAAYA 271
              AW IG+DMNLLA+AYA
Sbjct: 247 NLSAWNIGFDMNLLASAYA 252

BLAST of Carg03498 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 248.8 bits (634), Expect = 4.9e-66
Identity = 126/261 (48.28%), Postives = 177/261 (67.82%), Query Frame = 0

Query: 10  LLQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERK 69
           L+ + KPY+ +IS+QFGYAGM II+ +SL HGM+HYVL VYRHA AT V+APFAL  ERK
Sbjct: 5   LMNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERK 64

Query: 70  MRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRM 129
           +RPK+T +IF Q+ +L  + P++DQN YY+G+  TS TFA A +N +P++TF++A++ R+
Sbjct: 65  IRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRL 124

Query: 130 EKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASN 189
           E +  K VR  AKV GT++T+ GA+LMT YKG +++F   G G    +     +   A  
Sbjct: 125 ESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGA 184

Query: 190 LHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAM 249
              D   I G++ L+  T  WA FFILQ  T+++Y A LSLTT++C +GTL+   V+L  
Sbjct: 185 AAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVT 244

Query: 250 EHRPHAWAIGWDMNLLAAAYA 271
                AW IG+D NL AAAY+
Sbjct: 245 VRDLSAWKIGFDSNLFAAAYS 265

BLAST of Carg03498 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 235.7 bits (600), Expect = 4.3e-62
Identity = 125/261 (47.89%), Postives = 178/261 (68.20%), Query Frame = 0

Query: 11  LQNAKPYIVVISLQFGYAGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKM 70
           ++ A+P+I ++ LQ G AGM+I+S   LN GMS+YVLVVYRHA AT+VMAPFA  F++K+
Sbjct: 10  MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKV 69

Query: 71  RPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTIPSMTFIMALLCRME 130
           RPK+TL IF ++ +L LL P+IDQN YYLG+K T+ TFA A+ N +P++TF++A +  +E
Sbjct: 70  RPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLE 129

Query: 131 KLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAAASNL 190
           +++L+ +R   KV GT+ T+ GAM+MT  KG V++ FWT      NT        A +++
Sbjct: 130 RVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNT--------AGTDI 189

Query: 191 HNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTLAME 250
           H+    IKG++ + I   ++A F ILQ IT+R Y A LSLT  +C +GT++   V L ME
Sbjct: 190 HS---AIKGAVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVME 249

Query: 251 H-RPHAWAIGWDMNLLAAAYA 271
              P AWAIGWD  LL A Y+
Sbjct: 250 KGNPSAWAIGWDTKLLTATYS 259