Carg02246 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02246
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	Carg_Chr15: 1452823 .. 1453509 (-)
RNA-Seq Expression	Carg02246
Synteny	Carg02246

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGATTCCGACTCTTCTTCCTCTCCTCCGCCGTCGCTGCCCCCGCCTCGCAGCCTCTCTATGCTTCTCCCCTTTCTGCTTGGCCTTGCCCGAACCACCCCTGGATCGCACCTCAACCAGATCGTTGTTCTCGATCATGCTGCCCGAACGGTCGTCGTTTTTGAAGGTCGTGTTCTTGAGTCTTTTCTCCGCGACTTGTCGGAGAAATCCGGGCCGTTGCCTGCATCCAAGGCTTCGATCGATGCGATGCCGAGAGTCGGGATTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGACGGATTTGAAGTCGGTGCGGAGGCGAGAGAGATGCCTTGCAAACATCTGTATCATTCGGATTGTATTGAGAAATGGTTGAGGGTTCGTGGAACGTGTCCGATTTGTCGGTTCACGATGCCGGTGGACGATCAGGAAGGGAAGAAGGACGATGATGAAGATATGGAAGGAGACGAAGGCCGAGTGGTTGTAGTGAATCTATGGGTTAACGACGGTGAACCAATGGATGTAGAATCGGTGGAATCGGGCTTGGGGCATGGATCGGGGGACTCCGATTCGGAATCTCACGATGAAATTACGGTCGAAGTTGACGCCTCATCAACGCAAGAACAAGATCCCAGTTCTGAAGATTCTTCGTCATTTTCATTTTCGTCCTTCTTCAACTAG

mRNA sequence

ATGTCCGATTCCGACTCTTCTTCCTCTCCTCCGCCGTCGCTGCCCCCGCCTCGCAGCCTCTCTATGCTTCTCCCCTTTCTGCTTGGCCTTGCCCGAACCACCCCTGGATCGCACCTCAACCAGATCGTTGTTCTCGATCATGCTGCCCGAACGGTCGTCGTTTTTGAAGGTCGTGTTCTTGAGTCTTTTCTCCGCGACTTGTCGGAGAAATCCGGGCCGTTGCCTGCATCCAAGGCTTCGATCGATGCGATGCCGAGAGTCGGGATTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGACGGATTTGAAGTCGGTGCGGAGGCGAGAGAGATGCCTTGCAAACATCTGTATCATTCGGATTGTATTGAGAAATGGTTGAGGGTTCGTGGAACGTGTCCGATTTGTCGGTTCACGATGCCGGTGGACGATCAGGAAGGGAAGAAGGACGATGATGAAGATATGGAAGGAGACGAAGGCCGAGTGGTTGTAGTGAATCTATGGGTTAACGACGGTGAACCAATGGATGTAGAATCGGTGGAATCGGGCTTGGGGCATGGATCGGGGGACTCCGATTCGGAATCTCACGATGAAATTACGGTCGAAGTTGACGCCTCATCAACGCAAGAACAAGATCCCAGTTCTGAAGATTCTTCGTCATTTTCATTTTCGTCCTTCTTCAACTAG

Coding sequence (CDS)

ATGTCCGATTCCGACTCTTCTTCCTCTCCTCCGCCGTCGCTGCCCCCGCCTCGCAGCCTCTCTATGCTTCTCCCCTTTCTGCTTGGCCTTGCCCGAACCACCCCTGGATCGCACCTCAACCAGATCGTTGTTCTCGATCATGCTGCCCGAACGGTCGTCGTTTTTGAAGGTCGTGTTCTTGAGTCTTTTCTCCGCGACTTGTCGGAGAAATCCGGGCCGTTGCCTGCATCCAAGGCTTCGATCGATGCGATGCCGAGAGTCGGGATTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGACGGATTTGAAGTCGGTGCGGAGGCGAGAGAGATGCCTTGCAAACATCTGTATCATTCGGATTGTATTGAGAAATGGTTGAGGGTTCGTGGAACGTGTCCGATTTGTCGGTTCACGATGCCGGTGGACGATCAGGAAGGGAAGAAGGACGATGATGAAGATATGGAAGGAGACGAAGGCCGAGTGGTTGTAGTGAATCTATGGGTTAACGACGGTGAACCAATGGATGTAGAATCGGTGGAATCGGGCTTGGGGCATGGATCGGGGGACTCCGATTCGGAATCTCACGATGAAATTACGGTCGAAGTTGACGCCTCATCAACGCAAGAACAAGATCCCAGTTCTGAAGATTCTTCGTCATTTTCATTTTCGTCCTTCTTCAACTAG

Protein sequence

MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVLESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN

Homology

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BLAST of Carg02246 vs. NCBI nr
Match: KAG6578580.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016134.1 E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 448.4 bits (1152), Expect = 3.8e-122
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

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BLAST of Carg02246 vs. NCBI nr
Match: XP_023551536.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 446.4 bits (1147), Expect = 1.4e-121
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

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BLAST of Carg02246 vs. NCBI nr
Match: XP_022939130.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata])

HSP 1 Score: 445.7 bits (1145), Expect = 2.5e-121
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDE RVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

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BLAST of Carg02246 vs. NCBI nr
Match: XP_022993365.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima])

HSP 1 Score: 445.7 bits (1145), Expect = 2.5e-121
Identity = 226/228 (99.12%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKH+YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

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BLAST of Carg02246 vs. NCBI nr
Match: KAA0039492.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 360.9 bits (925), Expect = 8.0e-96
Identity = 195/238 (81.93%), Postives = 204/238 (85.71%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELG DCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE-------GKKDDDEDMEG---DEGRVVVVNLWVN 180
           DCIEKWL VRGTCP+CRF MPVDD+        G  ++DEDMEG    EGRVVVVNLWVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVN 180

Query: 181 DGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           DGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 DGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 234

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BLAST of Carg02246 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.4e-23
Identity = 72/187 (38.50%), Postives = 99/187 (52.94%), Query Frame = 0


Query: 18  RSLSMLLPFLLGLARTTP--------GSHLNQIVVLDHAARTVVVFEGRV-LESFLRDL- 77
           R L + LPFLLG +             S   +I++++   + ++V EG   +   LR L 
Sbjct: 24  RDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRSLL 83

Query: 78  -SEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEKW 137
            S + G  PASKASIDAMP V I     +C ICL+ ++     +EMPCKH +H  CIEKW
Sbjct: 84  ESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIEKW 143

Query: 138 LRVRGTCPICRFTMPVD-DQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESVESGLG 193
           L   G+CP+CR+ MPVD D+ GKK +D       G  + V    NDG  +   S + G  
Sbjct: 144 LGFHGSCPVCRYEMPVDGDEIGKKRND-------GNEIWVRFSFNDGRRIRDFSAQDG-- 198

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BLAST of Carg02246 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.9e-21
Identity = 45/101 (44.55%), Postives = 66/101 (65.35%), Query Frame = 0


Query: 55  FEGRVLESFLRDLSE----KSGPLPASKASIDAMPRVGITELGMD-----CAICLDGFEV 114
           F G  LE  ++ L+E    + G  PASK++I+A+P V IT+  ++     CA+C+D FE 
Sbjct: 174 FIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEE 233

Query: 115 GAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQE 147
           G EA++MPCKHLYH DC+  WL +  +CP+CR  +P DD +
Sbjct: 234 GTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPD 274

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BLAST of Carg02246 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.2e-19
Identity = 52/147 (35.37%), Postives = 75/147 (51.02%), Query Frame = 0


Query: 54  VFEGRVLESFLRDLSE---------KSGPLPASKASIDAMPRVGI----TELGMDCAICL 113
           +  G   E  L  LS+         +SG  PASK++I+++PRV I    T+   +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 114 DGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEG 173
           + FE G E REMPCKH++H DCI  WL +R +CP+CRF +P         D      +E 
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP--------SDPIQRSNEEE 279

Query: 174 RVVVVNLWVNDGEPMDVESVESGLGHG 188
             V + +W   G    V    +G+  G
Sbjct: 280 HAVGMTIWRLPGGGFAVGRFNAGVREG 298

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BLAST of Carg02246 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.7e-19
Identity = 46/123 (37.40%), Postives = 72/123 (58.54%), Query Frame = 0


Query: 55  FEGRVLESFLRDLSE----KSGPLPASKASIDAMPRVGITELGM-----DCAICLDGFEV 114
           F G  LE  ++ L+E    + G  PASK++IDA+P V +T+  +      CA+C+D FE 
Sbjct: 166 FFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED 225

Query: 115 GAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDD---------QEGKKDDDEDME 160
           G++ ++MPCKH++H DC+  WL +  +CP+CRF +P DD          +G  D    +E
Sbjct: 226 GSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVE 285

[Close]

BLAST of Carg02246 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.9e-17
Identity = 63/194 (32.47%), Postives = 96/194 (49.48%), Query Frame = 0


Query: 54  VFEGRVLESFLRDLSE---------KSGPLPASKASIDAMPRVGITELGM----DCAICL 113
           +  G   E  L  LS+         +SG  PASK++I+++PRV I++  +    +CA+C 
Sbjct: 144 ILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCT 203

Query: 114 DGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDED------ 173
           + FE   EAREMPCKHL+H DCI  WL +R +CP+CRF +P +      +++ED      
Sbjct: 204 EIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNAVGMT 263

Query: 174 ---MEGDEGRVVVVNLWVNDGE---PMDVESVESGLGHGSGDSDSESHDEITVEVDASST 223
              + G    V   N  + DGE   P+ +  ++     G G  +S+        V +  T
Sbjct: 264 IWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMD-----GGGIGNSQDGPRRISWVRSHGT 323

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BLAST of Carg02246 vs. ExPASy TrEMBL
Match: A0A6J1JYB9 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489408 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.2e-121
Identity = 226/228 (99.12%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKH+YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

[Close]

BLAST of Carg02246 vs. ExPASy TrEMBL
Match: A0A6J1FLT0 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC111445129 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.2e-121
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDE RVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

[Close]

BLAST of Carg02246 vs. ExPASy TrEMBL
Match: A0A5A7T7P7 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00210 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.9e-96
Identity = 195/238 (81.93%), Postives = 204/238 (85.71%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELG DCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE-------GKKDDDEDMEG---DEGRVVVVNLWVN 180
           DCIEKWL VRGTCP+CRF MPVDD+        G  ++DEDMEG    EGRVVVVNLWVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVN 180

Query: 181 DGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           DGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 DGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 234

[Close]

BLAST of Carg02246 vs. ExPASy TrEMBL
Match: A0A0A0KS87 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 195/244 (79.92%), Postives = 206/244 (84.43%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELGMDCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE--------GKKDDDEDM------EGDEGRVVVVN 180
           DCIEKWL VRGTCP+CRF MPVDD+         G ++++EDM      EG EGRVVVVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFAMPVDDRRKDGGGGGGGDEEEEEDMEGGEGGEGGEGRVVVVN 180

Query: 181 LWVNDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASST--QEQDPSSEDSSSFSFS 229
           LWVNDGEPMD   VESGL HGS DSDSES DEI VEVD SST  QEQDPSSEDSSSFSFS
Sbjct: 181 LWVNDGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQEQDPSSEDSSSFSFS 240

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BLAST of Carg02246 vs. ExPASy TrEMBL
Match: A0A1S3CC03 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.2e-89
Identity = 181/224 (80.80%), Postives = 190/224 (84.82%), Query Frame = 0


Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELG DCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE-------GKKDDDEDMEG---DEGRVVVVNLWVN 180
           DCIEKWL VRGTCP+CRF MPVDD+        G  ++DEDMEG    EGRVVVVNLWVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVN 180

Query: 181 DGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPS 215
           DGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPS
Sbjct: 181 DGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS 220

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BLAST of Carg02246 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-24
Identity = 72/187 (38.50%), Postives = 99/187 (52.94%), Query Frame = 0


Query: 18  RSLSMLLPFLLGLARTTP--------GSHLNQIVVLDHAARTVVVFEGRV-LESFLRDL- 77
           R L + LPFLLG +             S   +I++++   + ++V EG   +   LR L 
Sbjct: 24  RDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRSLL 83

Query: 78  -SEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEKW 137
            S + G  PASKASIDAMP V I     +C ICL+ ++     +EMPCKH +H  CIEKW
Sbjct: 84  ESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIEKW 143

Query: 138 LRVRGTCPICRFTMPVD-DQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESVESGLG 193
           L   G+CP+CR+ MPVD D+ GKK +D       G  + V    NDG  +   S + G  
Sbjct: 144 LGFHGSCPVCRYEMPVDGDEIGKKRND-------GNEIWVRFSFNDGRRIRDFSAQDG-- 198

[Close]

BLAST of Carg02246 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.8e-22
Identity = 45/120 (37.50%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 66  DLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEK 125
           DL+ + G  PA+K +++A+  V I +  + C++CLD FE+G EA+EMPCKH +HSDC+  
Sbjct: 195 DLNNRYGTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLP 254

Query: 126 WLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNL-WVNDGEPMDVESVESGL 185
           WL +  +CP+CR+ +P  D +  K D E    D+    + N    ++G   D  S  S +
Sbjct: 255 WLELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSSV 314

[Close]

BLAST of Carg02246 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.8e-22
Identity = 45/120 (37.50%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 66  DLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEK 125
           DL+ + G  PA+K +++A+  V I +  + C++CLD FE+G EA+EMPCKH +HSDC+  
Sbjct: 195 DLNNRYGTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLP 254

Query: 126 WLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNL-WVNDGEPMDVESVESGL 185
           WL +  +CP+CR+ +P  D +  K D E    D+    + N    ++G   D  S  S +
Sbjct: 255 WLELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSSV 314

[Close]

BLAST of Carg02246 vs. TAIR 10
Match: AT3G13430.3 (RING/U-box superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.8e-22
Identity = 45/120 (37.50%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 66  DLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEK 125
           DL+ + G  PA+K +++A+  V I +  + C++CLD FE+G EA+EMPCKH +HSDC+  
Sbjct: 195 DLNNRYGTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLP 254

Query: 126 WLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNL-WVNDGEPMDVESVESGL 185
           WL +  +CP+CR+ +P  D +  K D E    D+    + N    ++G   D  S  S +
Sbjct: 255 WLELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSSV 314

[Close]

BLAST of Carg02246 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.5e-20
Identity = 52/147 (35.37%), Postives = 75/147 (51.02%), Query Frame = 0


Query: 54  VFEGRVLESFLRDLSE---------KSGPLPASKASIDAMPRVGI----TELGMDCAICL 113
           +  G   E  L  LS+         +SG  PASK++I+++PRV I    T+   +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 114 DGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEG 173
           + FE G E REMPCKH++H DCI  WL +R +CP+CRF +P         D      +E 
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP--------SDPIQRSNEEE 279

Query: 174 RVVVVNLWVNDGEPMDVESVESGLGHG 188
             V + +W   G    V    +G+  G
Sbjct: 280 HAVGMTIWRLPGGGFAVGRFNAGVREG 298

The following BLAST results are available for this feature:

BLAST of Carg02246 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578580.1	3.8e-122	100.00	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023551536.1	1.4e-121	99.56	E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo]	[more]
XP_022939130.1	2.5e-121	99.56	E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata]	[more]
XP_022993365.1	2.5e-121	99.12	E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima]	[more]
KAA0039492.1	8.0e-96	81.93	E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3...	[more]

BLAST of Carg02246 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	1.4e-23	38.50	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
P0CH30	3.9e-21	44.55	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9SNB6	2.2e-19	35.37	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q8LPN7	3.7e-19	37.40	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q940T5	5.9e-17	32.47	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of Carg02246 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JYB9	1.2e-121	99.12	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A6J1FLT0	1.2e-121	99.56	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A5A7T7P7	3.9e-96	81.93	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KS87	1.2e-94	79.92	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 P...	[more]
A0A1S3CC03	1.2e-89	80.80	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 P...	[more]

BLAST of Carg02246 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	1.0e-24	38.50	RING/U-box superfamily protein	[more]
AT3G13430.1	2.8e-22	37.50	RING/U-box superfamily protein	[more]
AT3G13430.2	2.8e-22	37.50	RING/U-box superfamily protein	[more]
AT3G13430.3	2.8e-22	37.50	RING/U-box superfamily protein	[more]
AT3G46620.1	1.5e-20	35.37	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 95..137 e-value: 0.0021 score: 13.0
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 96..136 e-value: 1.2E-8 score: 44.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 95..137 e-value: 3.3E-14 score: 53.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 96..137 score: 12.415513
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 63..143 e-value: 9.6E-21 score: 75.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 204..228
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..228
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 42..152
None	No IPR available	PANTHER	PTHR15710:SF132	E3 UBIQUITIN-PROTEIN LIGASE MPSR1	coord: 42..152
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 95..137 e-value: 6.22691E-21 score: 80.4237
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 95..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02246-RA	Carg02246-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding